Aquaculture and Fisheries最新文献_第8页

Black scabbardfish (Aphanopus carbo) longline fishery off the Portuguese continental coast: Spatial information in support of deep-sea fisheries regulations 葡萄牙大陆海岸外延绳钓黑鞘鱼：支持深海渔业法规的空间信息

Aquaculture and Fisheries Pub Date : 2025-11-01 Epub Date: 2025-04-24 DOI: 10.1016/j.aaf.2025.02.002

Aida Campos , Pedro Leitão , Luís Sousa , Victor Henriques

{"title":"Black scabbardfish (Aphanopus carbo) longline fishery off the Portuguese continental coast: Spatial information in support of deep-sea fisheries regulations","authors":"Aida Campos , Pedro Leitão , Luís Sousa , Victor Henriques","doi":"10.1016/j.aaf.2025.02.002","DOIUrl":"10.1016/j.aaf.2025.02.002","url":null,"abstract":"<div><div>The main objective of this study is to provide, for the first time, fisheries-dependent, spatially explicit information on the activity for the black scabbard fish (<em>Aphanopus carbo</em>) longline fleet operating off the Portuguese coast. Fisheries monitoring data were analysed, covering the period 2014–2016, aiming at identifying deep-sea fishing grounds, gaining a better understanding of the fleet spatial dynamics, quantifying fishing effort and providing information on the species distribution and abundance. E-logbooks were used to define general patterns of fishing activity, while the combined analysis of Vessel Monitoring System (VMS) and Automatic Identification System (AIS) data allowed to spatially identify fishing trips and define haul-back trajectories corresponding to fishing events. A total of 19 vessels were found to be involved in this fishery, mainly operating at depths from 1000 to 1500 m, landing the black scabbard fish in two main ports located in the centre and three secondary ports in the northern region. Fishing effort and catch per unit of effort (CPUE) were estimated and mapped for the period in study and the geographic extension of the fishery in the Portuguese continental slope was highlighted. Vessels keep their activity spatially restricted, in a clear strategy to avoid competition; their areas of activity are contiguous and generally related to the closest port. Some recommendations are drawn in support of a continuous monitoring programme for this fishery.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 6","pages":"Pages 1069-1078"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential methylation reveals pathways associated with sex differentiation in Chinese tongue sole (Cynoglossus semilaevis) 中国舌底（Cynoglossus semiaevis）性别分化的差异甲基化途径

Aquaculture and Fisheries Pub Date : 2025-11-01 Epub Date: 2024-12-25 DOI: 10.1016/j.aaf.2024.12.002

Yaqun Zhang , Feng Xu , Hengde Li , Songlin Chen

{"title":"Differential methylation reveals pathways associated with sex differentiation in Chinese tongue sole (Cynoglossus semilaevis)","authors":"Yaqun Zhang , Feng Xu , Hengde Li , Songlin Chen","doi":"10.1016/j.aaf.2024.12.002","DOIUrl":"10.1016/j.aaf.2024.12.002","url":null,"abstract":"<div><div>Sex reversal is a complex biological phenomenon that occurs in <em>Cynoglossus semilaevis</em>. Some genetic females may irreversibly convert to pseudomales, thus increasing aquaculture costs because males grow much more slowly than females. In recent years, our studies have shown that genetic background is the primary factor contributing to sex reversal in tongue sole, and two linked loci on the Z chromosome were found to be associated with sex reversal. However, the role of epigenetics in sex reversal and temperature-independent sex determination has yet to be determined. In this study, we used reduced representation bisulfite sequencing (RRBS) to investigate the differences in methylation among female, male and pseudomale tongue sole and explored the possible pattern of DNA methylation associated with sex differentiation and sex reversal. A total of 32200, 341115 and 2691 DMRs with average lengths of 163.45, 170.15 and 128.77 bp, respectively, were identified between pseudomales and females, males and females, and pseudomales and males. Notably, the sex chromosome (Chr. Z) contained the greatest number of DMRs (303) between pseudomales and males. All the DMGs in each pairwise comparison were enriched in ubiquitin-mediated proteolysis, biosynthesis of secondary metabolites, thermogenesis and metabolic pathways, etc. In addition, differentially methylated genes possibly related to sex differentiation, such as <em>dmrt1</em>, <em>nobox</em>, <em>foxl2</em>, <em>pdgfrb</em>, <em>rspo1</em>, <em>lhx9</em>, <em>emx2</em>, <em>arx</em>, and <em>piwil2,</em> were screened. Methylation differential genes and metabolic pathways associated with sex differentiation have been identified, suggesting the vital role of methylation in gender reversal and sex determination. This study provides valuable insight into epigenetic mechanisms involved in sex differentiation and sex reversal in <em>C. semilaevis</em>.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 6","pages":"Pages 945-952"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145289993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in Acipenser ruthenus liver structure during domestication: Preliminary data 粗斑鲟在驯化过程中肝脏结构的变化：初步数据

Aquaculture and Fisheries Pub Date : 2025-11-01 Epub Date: 2025-04-23 DOI: 10.1016/j.aaf.2025.03.002

Nataliia Matviienko , Danguole Montvydienė , Nijole Kazlauskienė , Živilė Jurgelėnė , Alexander Didenko , Mykhailo Koziy

引用次数: 0

Establishment a cell line of splenic fibroblasts from golden pompano and its application in heavy metal toxicology and pathogen infection 金鲳鱼脾成纤维细胞细胞系的建立及其在重金属毒理学和病原体感染研究中的应用

Aquaculture and Fisheries Pub Date : 2025-11-01 Epub Date: 2024-10-02 DOI: 10.1016/j.aaf.2024.09.001

Lin Huang , Mingzhu Liu , Xiaohua Lu , Jihui Kuang , Libo Zhu , Yunyi Wei , Ke Ke , Hao Wang , Tianyi Lv , Jun Xiao , Qing Yu , Pengfei Li

{"title":"Establishment a cell line of splenic fibroblasts from golden pompano and its application in heavy metal toxicology and pathogen infection","authors":"Lin Huang , Mingzhu Liu , Xiaohua Lu , Jihui Kuang , Libo Zhu , Yunyi Wei , Ke Ke , Hao Wang , Tianyi Lv , Jun Xiao , Qing Yu , Pengfei Li","doi":"10.1016/j.aaf.2024.09.001","DOIUrl":"10.1016/j.aaf.2024.09.001","url":null,"abstract":"<div><div>A cell line derived from golden pompano <em>Trachinotus ovatus</em> splenic fibroblasts (TOSF) was established, its application in toxicology and virus susceptibility was demonstrated in this study. The TOSF cells grew fastest with the L-15 medium containing 10% fetal bovine serum concentration at 28 °C. The chromosomal number of normal TOSF cells was 2N = 48. TOSF cells showed high sensitivity to grouper iridovirus (SGIV) and nervous necrosis virus (NNV). Cadmium and copper had toxic effects on TOSF cells, which could cause an oxidative stress response in TOSF cells and activate the antioxidant system to regulate the reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) level in the cells. Cadmium and copper had an impact on the replication of SGIV and NNV in the TOSF cells, and copper could limit the proliferation of <em>Aeromonas hydrophila</em> in TOSF cells. Generally, the TOSF cell line can apply for heavy metal toxicity mechanisms study and environment monitoring, as well as the pathogen infection mechanism research.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 6","pages":"Pages 1050-1061"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of dietary chitin on Atlantic salmon (Salmo salar) chitinase activity, gene expression, and microbial composition 饲料中几丁质对大西洋鲑鱼几丁质酶活性、基因表达和微生物组成的影响

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2024-11-18 DOI: 10.1016/j.aaf.2024.10.005

Matilde Mengkrog Holen , Simen Rød Sandve , Thomas Nelson Harvey , Yang Jin , Inga Leena Angell , Knut Rudi , Matthew Peter Kent

引用次数: 0

Genome-wide identification of salinity stress-associated lncRNAs in Nile tilapia 尼罗罗非鱼盐度胁迫相关 lncRNA 的全基因组鉴定

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2024-05-09 DOI: 10.1016/j.aaf.2024.04.005

Dandan Huang , Xueying Liang , Chunhui Ai , Zhimou Liu , Juping Wen , Zhenbin Wang , Junhong Xia

{"title":"Genome-wide identification of salinity stress-associated lncRNAs in Nile tilapia","authors":"Dandan Huang , Xueying Liang , Chunhui Ai , Zhimou Liu , Juping Wen , Zhenbin Wang , Junhong Xia","doi":"10.1016/j.aaf.2024.04.005","DOIUrl":"10.1016/j.aaf.2024.04.005","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) are increasingly recognized as key players in various biological processes, yet their role in fish's response to salinity changes remains largely unexplored. This study undertakes a genome-wide analysis of lncRNAs in Nile tilapia (<em>Oreochromis niloticus</em>), examining their response to salinity stress across a substantial dataset of 98 RNA-seq samples from nine different tissues. We identified 28,819 high-confidence lncRNA candidates, which are characterized by a shorter average length of 788 nucleotides and fewer exons (average 1.84) compared to mRNAs. Specifically, in the gills exposed to salinity 27 for three and a half months, we found 846 differentially expressed lncRNAs (DElncRNAs), including 342 upregulated and 504 downregulated transcripts. The study also highlighted 220 significant DElncRNA-mRNA pairs, indicating potential regulatory interactions. Notably, in response to acute salinity stress, we identified 989 DElncRNAs, with 414 upregulated and 575 downregulated in gills. An analysis of these DElncRNAs revealed 348 high-confidence pairs, with Gene Ontology (GO) and KEGG pathway analyses indicating a prominent role in immune-inflammatory responses for most target genes, such as <em>trim7, trim35, trim47, cd225</em>. Crucially, two DElncRNAs (ENSONIT00000076723 and MSTRG.18195.2) were found to co-localize with QTL intervals associated with salinity tolerance, situated near three significant SNPs. Additionally, a notable <em>cis</em>-acting pair, consisting of mRNA ENSONIT00000087370 (<em>apold1a</em>) and lncRNA ENSONIT00000076723, demonstrated a strong correlation under both acute and chronic hypo-osmotic stress. Our findings significantly broaden the understanding of lncRNAs in salinity stress adaptation and suggest their novel roles as key regulators in the osmoregulatory mechanisms of Nile tilapia.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 771-782"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141056979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in cathepsin b is associated with growth in Asian seabass 组织蛋白酶b的多态性与亚洲鲈鱼的生长有关

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2025-06-18 DOI: 10.1016/j.aaf.2025.05.003

Shadame Yeo , Joey Wong , Y.X. Tay , Zituo Yang , Le Wang , Fei Sun , May Lee , Yanfei Wen , Gen Hua Yue

{"title":"Polymorphisms in cathepsin b is associated with growth in Asian seabass","authors":"Shadame Yeo , Joey Wong , Y.X. Tay , Zituo Yang , Le Wang , Fei Sun , May Lee , Yanfei Wen , Gen Hua Yue","doi":"10.1016/j.aaf.2025.05.003","DOIUrl":"10.1016/j.aaf.2025.05.003","url":null,"abstract":"<div><div>This study investigates the relationship between polymorphisms in the cathepsin B (<em>ctsb</em>) gene and body weight in Asian seabass (<em>Lates calcarifer</em>). Despite the economic significance of this species, the genetic factors that influence growth performance in fish remain poorly understood. CTSB, a lysosomal cysteine protease involved in protein degradation and tissue remodelling, is a crucial regulator of growth. We analysed the full-length cDNA of <em>ctsb</em> and mapped it to the major quantitative trait locus (QTL) for growth on linkage group 2 in <em>L. calcarifer</em>. Single nucleotide polymorphisms (SNPs) within ctsb were identified and their associations with body weight were analysed in 298 individuals. Results revealed that SNP1 (C > T) in intron 2 of <em>ctsb</em> was significantly correlated with body weight. Quantitative real-time PCR (qRT-PCR) showed that <em>ctsb</em> was expressed in all tissues, with the highest expression in the gills and kidney of developing seabass. Knockdown/overexpression of <em>ctsb</em> in cell lines respectively promoted/inhibited cell proliferation without altering cell size. These findings suggest that <em>ctsb</em> plays a significant role in growth regulation in Asian seabass. This study provides a potential molecular marker for selecting fast-growing Asian seabass. Future research should focus on identifying causal variants in ctsb associated with accelerated growth, conducting in vivo studies, and exploring related signalling pathways.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 764-770"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology-based selective breeding strategy analysis for abdominal meat yield in Procambarus clarkii 基于形态学的克氏原鲤腹肉产量选育策略分析

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2024-05-10 DOI: 10.1016/j.aaf.2024.04.009

Qishuai Wang , Qian Hu , Siqi Yang , Ruixue Shi , Feifei Zheng , Xiaolong Liu , Jiangfeng Huang , Yanhe Li

{"title":"Morphology-based selective breeding strategy analysis for abdominal meat yield in Procambarus clarkii","authors":"Qishuai Wang , Qian Hu , Siqi Yang , Ruixue Shi , Feifei Zheng , Xiaolong Liu , Jiangfeng Huang , Yanhe Li","doi":"10.1016/j.aaf.2024.04.009","DOIUrl":"10.1016/j.aaf.2024.04.009","url":null,"abstract":"<div><div>The edible portion of <em>Procambarus clarkii</em> mainly consists of the abdominal muscles, highlighting the genetic enhancement of abdominal meat yield in breeding. To explore a selective breeding strategy for abdominal meat yield, the correlation of morphological characteristics with abdominal meat yield based on five <em>P. clarkii</em> populations collected from its major production areas in China was analyzed and an optimal prediction model was constructed for predicting abdominal meat yield. With the analyses of <em>P. clarkii</em> morphological characteristics, Fulton's condition factor (K), which had a strong negative correlation (-0.800 in males and - 0.705 in females) with abdominal meat yield and the advantage of not requiring the sacrifice of breeding candidates, was eventually selected as the morphological predictor. And the optimal prediction model constructed based on K value was a quadratic curve, with <em>R</em><sup>2</sup> values of 0.684 for males and 0.590 for females, and correlation coefficients of 0.827 and 0.768 between observed and predicted values for males and females, respectively. The results of the abdominal meat yield selective breeding experiment, utilizing the optimal prediction model, demonstrated that the breeding population exhibited favorable morphological variation as expected. These findings provide a morphology-based selection strategy for breeding the abdominal meat yield of <em>P. clarkii</em>.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 878-886"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141038292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological strategies in aquaculture disease management: Towards a sustainable blue revolution 水产养殖疾病管理的生物学策略：走向可持续的蓝色革命

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2025-07-03 DOI: 10.1016/j.aaf.2025.04.002

Keng Chin Lim , Fatimah Md Yusoff , Fatin M.I. Natrah , Mahanama De Zoysa , Ina Salwany Md Yasin , Jasmin Yaminudin , Murni Karim

{"title":"Biological strategies in aquaculture disease management: Towards a sustainable blue revolution","authors":"Keng Chin Lim , Fatimah Md Yusoff , Fatin M.I. Natrah , Mahanama De Zoysa , Ina Salwany Md Yasin , Jasmin Yaminudin , Murni Karim","doi":"10.1016/j.aaf.2025.04.002","DOIUrl":"10.1016/j.aaf.2025.04.002","url":null,"abstract":"<div><div>Although the aquaculture industry has undergone monumental development worldwide, the ever-present threats of infectious diseases have become a constraining factor, imperiling its sustainability. Antimicrobial resistance (AMR) remains a real menace to industrial aquaculture due to the careless adoption of preventive therapies (antimicrobial therapeutic drugs) to forestall disease outbreaks in aquatic food production. Suitable strategies, or at least supplementary measures, should therefore be developed to curb the emergence and widespread transmission of AMR. Vaccination represents one of the primary options to substantially mitigate the economic damages imposed by emerging infectious diseases on global aquaculture; nevertheless, the availability of commercial aquatic vaccines is usually limited, and many vaccines only confer minimal or poor protection against infections (during the early stages of animal development). Accordingly, a large body of research has been enthusiastically exploring alternate approaches for managing animal health challenges. These efforts have led to the establishment of various biocontrol strategies, such as the versatile use of high-value functional ingredients (e.g., probiotics, prebiotics, synbiotics, paraprobiotics, postbiotics, and phytogenics), phage therapy, and quorum-sensing interference (QSI), to promote the health and welfare of farmed aquatic species in a responsive or preventative manner. This review article addresses the state-of-the-art pertinent to biological control as an eco-friendly green approach for aquatic disease management, paving the route to a sustainable blue revolution. The potential biological mechanisms of these strategies are also described, along with the impediments to scientific progress and topics that merit further investigation.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 743-763"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting hybrid vigor to enhance survival and growth for whiteleg shrimp (Litopenaeus vannamei): A complete diallel cross experiment 利用杂交活力提高南美白对虾（Litopenaeus vannamei）的存活率和生长：一个完整的杂交实验

Aquaculture and Fisheries Pub Date : 2025-09-01 Epub Date: 2024-05-08 DOI: 10.1016/j.aaf.2024.04.004

Zhangwu Yang , Zhe Zhang , Guodong Wang , Zhongchi Huang , Weiwei You

{"title":"Exploiting hybrid vigor to enhance survival and growth for whiteleg shrimp (Litopenaeus vannamei): A complete diallel cross experiment","authors":"Zhangwu Yang , Zhe Zhang , Guodong Wang , Zhongchi Huang , Weiwei You","doi":"10.1016/j.aaf.2024.04.004","DOIUrl":"10.1016/j.aaf.2024.04.004","url":null,"abstract":"<div><div>Whiteleg shrimp (<em>Litopenaeus vannamei</em>) is an economically important cultured species that is extensively cultured in China. However, there is the potential for further optimization of its survival and growth traits that are key determinants of productivity in aquaculture. This study investigated the efficacy of hybridization, a genetic improvement technology leveraging hybrid vigor, as a strategy for enhancing these traits in <em>L</em>. <em>vannamei</em>. The study was conducted using a 4 × 4 complete diallel cross experiment involving four selected strains (5, 8, 9, and 10), with the aim of identifying potential crosses exhibiting hybrid vigor. The most suitable crosses were those between strains 8 and 9, where the mid-parent heterosis (<em>H</em><sub><em>89</em></sub>) was 16.52% for survival and 18.93% for growth. Our findings revealed that most reciprocal hybrids displayed higher rates of survival than their inbred parents. Additionally, an age-dependent increase in the heterosis effect on weight was observed over mid-parents and single-parents in most hybrid offspring. These findings suggest that hybridization could be a promising approach to improving the survival and growth of whiteleg shrimp. Our crossing experiment has yielded novel genetic resources for shrimp breeding, ultimately enhancing the economic importance of whiteleg shrimp.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 860-866"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0