Biosafety and Health最新文献

{"title":"Vaccinia virus Tiantan strain blocks host antiviral innate immunity and programmed cell death by disrupting gene expression","authors":"Changcheng Wu ,&nbsp;Zhongxian Zhang ,&nbsp;Zhaoqing Li ,&nbsp;Ruorui Li ,&nbsp;Shuting Huo ,&nbsp;Han Li ,&nbsp;Roujian Lu ,&nbsp;Houwen Tian ,&nbsp;Wenling Wang ,&nbsp;Li Zhao ,&nbsp;Baoying Huang ,&nbsp;Yao Deng ,&nbsp;Wenjie Tan","doi":"10.1016/j.bsheal.2024.08.001","DOIUrl":"10.1016/j.bsheal.2024.08.001","url":null,"abstract":"<div><div>The vaccinia virus Tiantan (VTT) is widely utilized as a smallpox vaccine in China and holds significant importance in the prevention of diseases stemming from poxvirus infections. Nevertheless, few studies have investigated the influence of VTT infection on host gene expression. In this study, we constructed time series transcriptomic profiles of HeLa cells infected with both VTT and western reserve (WR) strains. We observed similar patterns of viral gene expression, while the expression levels of host genes varied between the two strains. There was an immediate and significant repression of host gene expression, particularly in genes associated with oxidative phosphorylation. Conversely, genes involved in nerve growth factor (NGF)-stimulated transcription were significantly activated. The upregulation of genes linked to the ribonucleic acid (RNA)-induced silencing complex (RISC) suggested a potential role for posttranscriptional regulation in the interaction between the vaccinia virus and the host. In the later stages of infection, pathways such as extracellular matrix organization, neutrophil degranulation, complement and interferon responses, translation, and programmed cell death are largely inhibited. A significant number of host genes exhibit correlations with changes in the expression levels of viral genes. The host genes that are negatively correlated with viral genes are mainly enriched in pathways associated with translation and the response to viral infection. This study significantly contributes to advancing our understanding of the dynamics between the vaccinia virus and the host, improving the application of VTTs and facilitating the development of effective vaccines against diseases such as smallpox and monkeypox.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 5","pages":"Pages 286-297"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and challenges of mpox detection technology","authors":"Wenjing Liu ,&nbsp;Erxin Zhang ,&nbsp;Wei Li ,&nbsp;Ruichen Lv ,&nbsp;Yanfeng Lin ,&nbsp;Yingjia Xu ,&nbsp;Jiameng Li ,&nbsp;Yuzhen Lai ,&nbsp;Yuxin Jiang ,&nbsp;Sijia Lin ,&nbsp;Xueqin Wang ,&nbsp;Peize Zhou ,&nbsp;Yue Song ,&nbsp;Wanpeng Shen ,&nbsp;Yiqian Sun ,&nbsp;Yuexi Li","doi":"10.1016/j.bsheal.2024.09.005","DOIUrl":"10.1016/j.bsheal.2024.09.005","url":null,"abstract":"<div><div>Mpox is a zoonotic disease caused by the monkeypox virus (MPXV). Diagnosing and treating the disease has become a global health concern requiring close attention to its spread to non-endemic regions. Clinical diagnosis is based on laboratory test results. Conventional detection techniques include real-time quantitative polymerase chain reaction (qPCR), genome sequencing, antigen and antibody identification, and virus isolation. Nevertheless, these methods fall short of rapidly and efficiently identifying MPXV, as they require specialized training, specific laboratory environments, and professional-grade equipment. Emerging technologies offer complementary advantages to meet diverse diagnostic needs, including various point-of-care testing (POCT) approaches and integrating biosensors with rapid detection techniques. This review discusses prospective future research avenues for MPXV detection, examining the advances and challenges of various detection techniques which may<!--> <!-->contribute to the ongoing elimination of mpox human-to-human<!--> <!-->transmission and serves as a reference<!--> <!-->for developing effective prevention and control strategies.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 5","pages":"Pages 260-269"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full genomic sequence characterization of the chikungunya virus from an imported case with serum viral concentration below culturable level","authors":"Mengling Jiang ,&nbsp;Muchun Wan ,&nbsp;Qinghong Fan ,&nbsp;Yuyi Min ,&nbsp;Guofang Tang ,&nbsp;Yingfen Wen ,&nbsp;Yaqing Lin ,&nbsp;Ruiying He ,&nbsp;Jiaojiao Li ,&nbsp;Yue Tang ,&nbsp;Yun Lan ,&nbsp;Feng Li","doi":"10.1016/j.bsheal.2024.09.003","DOIUrl":"10.1016/j.bsheal.2024.09.003","url":null,"abstract":"<div><div>Chikungunya virus (CHIKV) infection, responsible for chikungunya fever and occasionally severe symptoms, has emerged as an increasing global health concern following several large-scale outbreaks from Africa, Asia, Europe, and America. Over the past two decades, South and Southeast Asia regions have gradually become hot spots for outbreaks involving multiple CHIKV lineages. In China, most CHIKV infections are imported, making it crucial to trace the origins and transmission routes for effective prevention and control. In January 2024, a case of imported chikungunya fever was confirmed in Guangzhou City, Guangdong Province, China. However, the serum CHIKV viral concentration was too low for cultivation [reverse transcription-polymerase chain reaction (RT-PCR) detection, cycle threshold = 32.62]. Despite this, we successfully obtained the viral genome sequence directly from the whole blood sample using an optimized metatranscriptomic sequencing strategy, achieving a full-length viral genome with an average depth of 54.3X. Further analysis confirmed that the CHIKV virus belonged to the Asian lineage, traced to Timor-Leste, where an endemic CHIKV outbreak had been reported in January 2024, consistent with the patient’s travel history. Finally, we analyzed genetic evolutionary trends and amino acid site variations. This study highlights the identification of a CHIKV infection origin using direct whole-blood metatranscriptomic sequencing, a valuable method for rapidly sequencing low viral-load samples.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 5","pages":"Pages 304-309"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of pseudotyped human coronaviruses and detection of pre-existing antibodies in the human population","authors":"Qi Jiang ,&nbsp;Xi Wu ,&nbsp;Fangyu Dong ,&nbsp;Shan Qiao ,&nbsp;Qiaoyun Shi ,&nbsp;Changyong Jian ,&nbsp;Chen Chen ,&nbsp;Jiuyue Zhou ,&nbsp;Youchun Wang ,&nbsp;Weijin Huang","doi":"10.1016/j.bsheal.2024.09.002","DOIUrl":"10.1016/j.bsheal.2024.09.002","url":null,"abstract":"<div><div>In order to clarify the pre-exist immunity background of different human coronaviruses (HCoV), this study investigated the positive rate of spike (S) protein antibodies of HCoV, including HCoV- severe acute respiratory syndrome (SARS) −associated coronavirus (SARS-CoV-1), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Middle East respiratory syndrome coronavirus (MERS-CoV), HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43, before and after the Coronavirus Disease 2019 (COVID-19) outbreak. We utilized pseudotyped virus-based neutralization assays (PBNA) or enzyme-linked immunosorbent assays (ELISA) to detect antibody levels against HCoV in serum samples collected in 2009–2010 and 2023. The PBNA results showed that neutralizing antibodies against SARS-CoV-1 and the MERS-CoV were negative. In the serum samples from 2009 to 2010, neutralizing antibodies against SARS-CoV-2 (D614G) were negative, whereas in the serum samples from 2023, 73 samples (73 %) showed neutralizing reactions with the SARS-CoV-2 D614G strain, 96 samples (96 %) with the BA.5 strain, and 91 samples (91 %) with the BF.7 strain. Among pre-COVID-19 samples, 33 % (33/100) showed neutralizing reactions with HCoV-229E and 63 % (63/100) with HCoV-NL63. Among post-COVID-19 samples, 50 % (50/100) showed neutralizing reactions with HCoV-229E and 49 % (49/100) with HCoV-NL63. Due to the different receptors of alpha coronavirus genus compared to other beta coronavirus genus, neutralizing antibodies against HCoV-OC43 and HCoV-HKU1 virus cannot be detected by constructing corresponding pseudotyped virus. Binding antibodies against HCoV-OC43 and HCoV-HKU1 virus were detected using ELISA. The results revealed that among pre-COVID-19 samples, 83 % (83/100) and 45 % (45/100) had binding activity with HCoV-OC43 and HCoV-HKU1, respectively. Among post-COVID-19 samples, 100 % (100/100) and 81 % (81/100) had binding activity with HCoV-OC43 and HCoV-HKU1, respectively.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 5","pages":"Pages 279-285"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing an advanced course of vaccinology in middle-income countries: The case of China","authors":"Likui Wang ,&nbsp;Lei Guo ,&nbsp;Yunguo Liu ,&nbsp;Anna Du ,&nbsp;Shuo Zhao ,&nbsp;George F. Gao","doi":"10.1016/j.bsheal.2024.09.006","DOIUrl":"10.1016/j.bsheal.2024.09.006","url":null,"abstract":"","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 5","pages":"Pages 257-259"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between climatic factors and the flea index of two plague hosts in Xilingol League, Inner Mongolia Autonomous Region","authors":"Lu Zhang ,&nbsp;Zihao Wang ,&nbsp;Nan Chang ,&nbsp;Meng Shang ,&nbsp;Xiaohui Wei ,&nbsp;Ke Li ,&nbsp;Jinyu Li ,&nbsp;Xinchang Lun ,&nbsp;Haoqiang Ji ,&nbsp;Qiyong Liu","doi":"10.1016/j.bsheal.2024.07.004","DOIUrl":"10.1016/j.bsheal.2024.07.004","url":null,"abstract":"<div><p>Climatic factors are closely associated with the occurrence of vector-borne diseases, and they also influence the distribution of vectors. The occurrence of plague is closely related to the population dynamics of fleas and their host animals, as well as climatic conditions. This study focused on Xilingol League, utilizing climatic and flea index data from 2012 to 2021. Spearman correlation and \"Boruta\" importance analysis were conducted to screen for climatic variables. A generalized additive model (GAM) was employed to investigate the influence of climatic factors and rodent density on the flea index. GAM analysis revealed distinct trends in flea index among different rodent hosts. For <em>Meriones unguiculatus</em>, the flea index declined with increased density and with higher humidity, yet rose with greater lagged sunshine duration. For <em>Spermophilus dauricus</em>, an initial increase in flea index with density was observed, followed by a decrease, and a rise in the index was noted when ground temperatures were low. This study reveals the nonlinear interactions and lag effects among climatic factors, density, and flea index. Climatic factors and density variably influence the flea index of two <em>Yersinia pestis</em> hosts. This research advances the prediction and early warning efforts for plague control, providing a theoretical basis for rodent and flea eradication strategies.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 4","pages":"Pages 244-250"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000867/pdfft?md5=e87ef9b2923994883b1a0340cc1d0f5b&pid=1-s2.0-S2590053624000867-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141688706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of the benchmarking tool for evaluating the operation of biorepositories for pathogenic resource using a modified Delphi method","authors":"","doi":"10.1016/j.bsheal.2024.05.001","DOIUrl":"10.1016/j.bsheal.2024.05.001","url":null,"abstract":"<div><p>In recent years, as the infectious diseases caused by pathogens such as novel coronavirus and mpox (formerly called monkeypox) spread globally, the significance of identification, preservation, and sharing of pathogenic resources become prominent. Along with the rapid development of biorepositories, it is imperative to evaluate their operation in a scientific manner. By using the literature review and modified Delphi method, this study develops a benchmarking tool for the comprehensive evaluation of the operation of biorepositories for pathogenic resources. The effective response rates of both rounds of expert surveys were 100 %. The authority coefficients (Cr) were 0.82 and 0.85, respectively, manifesting the reliability of consultation results. In the second-round survey, the Kendall’s coefficient of concordance (Kendall’s W) of all indicators ranged from 0.09 to 0.31 (<em>P</em> &lt; 0.001), the comprehensive score ranged from 4.02 to 4.94, the standard deviation ranged from 0.21 to 0.77, and the coefficient of variation (CV) ranged from 0.04 to 0.22, indicating that the expert opinions reached consensus. The final benchmarking tool was composed of 4 primary indicators, 12 secondary indicators, and 65 tertiary indicators. The weights of the four primary indicators allocated through the rank-sum ratio method, namely organizational structure, management requirements, biobanking capacity, and sharing capacity, were 30.50 %, 30.08 %, 25.45 %, and 13.97 %, respectively. The benchmarking tool established in this study provides references for the comprehensive evaluation of the operation and puts forward advice for the sustainable development of biorepositories for pathogenic resources.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 4","pages":"Pages 199-205"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000806/pdfft?md5=63302128e2e0a2dcc03c37569419faf5&pid=1-s2.0-S2590053624000806-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141390892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated robot and artificial intelligence-powered wastewater surveillance for proactive mpox outbreak prediction","authors":"Guanyong Ou ,&nbsp;Yuxuan Tang ,&nbsp;Jiexiang Liu ,&nbsp;Yabin Hao ,&nbsp;Zhi Chen ,&nbsp;Ting Huang ,&nbsp;Shaxi Li ,&nbsp;Shiyu Niu ,&nbsp;Yun Peng ,&nbsp;Jiaqi Feng ,&nbsp;Hongwei Tu ,&nbsp;Yang Yang ,&nbsp;Han Zhang ,&nbsp;Yingxia Liu","doi":"10.1016/j.bsheal.2024.07.002","DOIUrl":"10.1016/j.bsheal.2024.07.002","url":null,"abstract":"<div><p>In the wake of the largest-ever recorded outbreak of mpox in terms of magnitude and geographical spread in human history since May 2022, we innovatively developed an automated online sewage virus enrichment and concentration robot for disease tracking. Coupled with an artificial intelligence (AI) model, our research aims to estimate mpox cases based on the concentration of the monkeypox virus (MPXV) in wastewater. Our research has revealed a compelling link between the levels of MPXV in wastewater and the number of clinically confirmed mpox infections, a finding that is reinforced by the ability of our AI prediction model to forecast cases with remarkable precision, capturing 87 % of the data’s variability. However, it is worth noting that this high precision in predictions may be related to the relatively high frequency of data acquisition and the relatively non-mobile isolated environment of the hospital itself. In conclusion, this study represents a significant step forward in our ability to track and respond to mpox outbreaks. It has the potential to revolutionize public health surveillance by utilizing innovative technologies for disease surveillance and prediction.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 4","pages":"Pages 225-234"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000855/pdfft?md5=2592a8d6cb43ddbe057733e65a5ea24f&pid=1-s2.0-S2590053624000855-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141709959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyberbiosecurity: Advancements in DNA-based information security","authors":"Tuoyu Liu ,&nbsp;Sijie Zhou ,&nbsp;Tao Wang ,&nbsp;Yue Teng","doi":"10.1016/j.bsheal.2024.06.002","DOIUrl":"10.1016/j.bsheal.2024.06.002","url":null,"abstract":"<div><p>Synthetic biology is a crucial component of the “cyber-biological revolution” in this new industrial revolution. Owing to breakthroughs in synthetic biology, deoxyribonucleic acid (DNA), the storehouse of hereditary material in biological systems, can now be used as a medium for storage (synthesis) and reading (sequencing) of information. However, integrating synthetic biology with computerization has also caused cyberbiosecurity concerns, encompassing biosecurity and information security issues. Malicious codes intended to attack computer systems can be stored as artificially synthesized DNA fragments, which can be released during DNA sequencing and decoding and attack computer and network systems. As these cyberbiosecurity threats become increasingly realistic, spreading awareness and information about how they can be prevented and controlled is crucial. This review aims to address this need by offering crucial theoretical backing for cyberbiosecurity research and raising awareness of risk mitigation and control measures in information security, biosecurity, and national security.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 4","pages":"Pages 251-256"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259005362400082X/pdfft?md5=824450e2816f527075568668e6a86411&pid=1-s2.0-S259005362400082X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142058248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An online survey among convalescents 5 months post SARS-CoV-2 infection in China","authors":"","doi":"10.1016/j.bsheal.2024.06.001","DOIUrl":"10.1016/j.bsheal.2024.06.001","url":null,"abstract":"<div><p>The effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection persist months and years after recovery. We conducted an online survey to assess the health condition of convalescents approximately 5 months following the primary infection of SARS-CoV-2. The study recruited 5,510 individuals who were primary infected, 626 participants who had experienced reinfection, and 521 participants who were without infective history. The most common disorders after the primary infection group were fatigue (15.18 %), memory issue (13.13 %), post-exertional malaise (PEM, 11.68 %), and brain fog (11.29 %) at the time of survey. In addition, SARS-CoV-2 infection had an impact on the reproductive systems. In stepwise logistic regression analysis, smoking currently, with background diseases, and outpatient visits in the acute phase could be associated with moderate / severe disorders. Further analysis of different background diseases showed that allergic rhinitis, hyperlipidemia, cardiovascular disease, autoimmune diseases, neurological diseases, and asthma likely increased the risk of moderate/severe disorders. The probability of developing disorders of individuals with SARS-CoV-2 reinfection was higher before the secondary infection than uninfected people. Fatigue, PEM, muscle pain/spasms, chills, joint pain, excessive sweating at rest, headache / dizziness, sore throat or foreign body sensation in the throat, cough, expectoration, dry / painful / watery eyes, loss of appetite and constipation were associated with an increased risk of reinfection. It was essential to undertake further research with enhanced randomization in a larger sample in the community, and to strengthen the validation of the research conclusions. The findings of this study contribute to a deeper understanding of the health recovery process among coronavirus disease 2019 (COVID-19) convalescents. Moreover, the findings help identify characteristic health risk factors associated with convalescents and highlight the risk of moderate / severe disorders and reinfection. Furthermore, the findings also provide valuable guidance and reference for SARS-CoV-2 rehabilitation strategies and the prevention of reinfection, offering insights for scientific recommendations.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 4","pages":"Pages 206-215"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000818/pdfft?md5=222f8c7f60b40b45e90486a1714fe850&pid=1-s2.0-S2590053624000818-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141400553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}