Biosafety and Health最新文献

{"title":"Exploring potential biosafety implications in DNA information storage","authors":"Shangzhe Li ,&nbsp;Yue Shi ,&nbsp;Jing Yang ,&nbsp;Haizhou Liu ,&nbsp;Lijia Jia ,&nbsp;Di Liu","doi":"10.1016/j.bsheal.2025.03.006","DOIUrl":"10.1016/j.bsheal.2025.03.006","url":null,"abstract":"<div><div>Deoxyribonucleic acid (DNA) information storage has emerged as a promising solution to address the challenges of traditional silicon-based data storage systems. However, the biosafety implications of artificially synthesized DNA sequences in this technology remain understudied. This research evaluates the biosafety risks associated with five representative DNA storage encoding methods [Church, Goldman, DNA Fountain, Grass, and movable-type (MT) encoding] by analyzing their sequence similarities to natural biological DNA. Through Kraken2 taxonomic classification and Basic Local Alignment Search Tool for nucleotides (BLASTn) alignment analysis, we found that while most artificially designed DNA sequences showed significant differences from known biological sequences, specific encoding methods produced sequences similar to natural genomes. The MT encoding method showed the highest annotation rate (4.59 %) in Kraken2 analysis, while Goldman and Fountain methods demonstrated significant local sequence alignments in BLASTn analysis. Sequence length positively correlated with annotation rates, suggesting longer sequences pose potentially higher biosafety risks. Furthermore, aligned sequences often exhibited characteristics of tandem repeats, particularly in non-coding regions. These findings highlight the importance of incorporating biosafety considerations in DNA storage encoding method development and suggest that randomization strategies may help mitigate potential risks. Our study provides valuable insights into the safe advancement of DNA storage technology and emphasizes the need for comprehensive biosafety evaluation in synthetic biology applications.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 2","pages":"Pages 132-139"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an in-house dual RT-qPCR assay for detecting SFTSV and Hantaan virus simultaneously","authors":"Xiaoyu Xue ,&nbsp;Youde Liu ,&nbsp;Chuan Song ,&nbsp;Tingting Liu ,&nbsp;Zishuai Liu ,&nbsp;Wenjing Niu ,&nbsp;Zhouling Jiang ,&nbsp;Yanli Xu ,&nbsp;Yuanyuan Zhang ,&nbsp;Ling Lin ,&nbsp;Zhihai Chen","doi":"10.1016/j.bsheal.2025.03.007","DOIUrl":"10.1016/j.bsheal.2025.03.007","url":null,"abstract":"<div><div>Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome (SFTS) and hemorrhagic fever with renal syndrome (HFRS), we developed a dual real‐time fluorescence‐based reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Recombinant plasmids and synthetic ribonucleic acid (RNA) were constructed to evaluate the specificity, sensitivity and reproducibility of the assay. Additionally, we assessed the specificity of the assay using samples from three distinct groups: individuals with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (n = 10), influenza A-positive individuals (n = 10), and healthy controls. Receiver operating characteristic (ROC) curves were used to assess diagnostic accuracy, while the Kappa coefficient and linear regression analysis were employed to evaluate clinical applicability. Our method exhibited specificity for both SFTSV and Hantaan virus detection, with detection limits of 333 and 1,022 copies/mL using plasmids, and 1,247 and 898 copies/mL using synthetic RNA, respectively. We evaluated 100 clinical samples from each of SFTS and HFRS. The Kappa coefficients for both diseases were 0.96. The areas under the ROC curves were 0.991 (<em>P</em> &lt; 0.001) and 0.989 (<em>P</em> &lt; 0.001), respectively. The linear regression equations were as follows: log (<em>y</em>) = 0.19 + 0.99 log (<em>x</em>) (<em>R²</em> = 0.95) for SFTS virus, and log (<em>y</em>) = 0.01 + 0.65 log (<em>x</em>) (<em>R²</em> = 0.92) for Hantaan virus. We established an in-house RT-qPCR method for the rapid quantification of both pathogens, making it an ideal tool for early clinical differentiation.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 2","pages":"Pages 110-116"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal analysis of cytokine dynamics in severe fever with thrombocytopenia syndrome patients — High-incidence regions of China (2010–2023)","authors":"Yanhan Wen ,&nbsp;Yeqing Tong ,&nbsp;Lei Gong ,&nbsp;Aqian Li ,&nbsp;Xiaoxia Huang ,&nbsp;Tingting Tian ,&nbsp;Tiezhu Liu ,&nbsp;Lina Sun ,&nbsp;Jiandong Li ,&nbsp;Dexin Li ,&nbsp;Mifang Liang ,&nbsp;Wei Wu ,&nbsp;Jiabing Wu ,&nbsp;Shiwen Wang","doi":"10.1016/j.bsheal.2025.03.005","DOIUrl":"10.1016/j.bsheal.2025.03.005","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia syndrome (SFTS) is a life-threatening tick-borne disease characterized by cytokine dysregulation and immune-mediated hyperinflammation. This multicenter retrospective study analyzed the dynamics of 17 cytokines across acute and recovery phases using 287 serum samples collected between 2010 and 2023 from high-incidence regions of China, evaluating their associations with disease severity, sex, age, and antibody responses. The results demonstrated that elevations of interleukin (IL)-6, interferon (IFN)-α, IL-8, and IFN-γ-induced protein 10 (IP-10) during the acute phase were associated with hyperinflammation, while IL-10 balanced inflammatory control and may have contributed to viral persistence. During recovery, most cytokines declined; however, IL-8 and IP-10 remained elevated longer in some patients, reflecting heterogeneity in recovery trajectories. Severe cases exhibited significantly higher levels of IL-10, IFN-γ, IL-6, IFN-α, tumor necrosis factor (TNF)-α, IL-8, and IP-10, underscoring their potential as biomarkers for disease severity prediction. Sex-based differences revealed higher IFN-γ and IL-8 levels in females, potentially due to hormonal and genetic factors, while older patients exhibited elevated IL-10, IFN-γ, and IFN-α, reflecting immune dysregulation and age-related shifts in adaptive immunity. Correlation analysis revealed distinct immune response patterns, with IL-10 strongly correlating with IFN-γ and minimal antibody-cytokine associations observed during the acute phase. In contrast, in the recovery phase, immunoglobulin G (IgG) negatively correlated with IL-10, IFN-γ, and IP-10, and immunoglobulin M (IgM) positively correlated with IL-10, IFN-γ, IL-6, IFN-α, TNF-α, IL-8, and IP-10, reflecting dynamic immune regulation and the interplay between humoral and cellular immunity. These findings provide critical insights into the immunopathogenesis of SFTS, supporting the development of cytokine-targeted therapies and advanced diagnostic tools to improve clinical outcomes.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 2","pages":"Pages 83-93"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of the mpox virus using a robust recombinase-aided amplification-based approach","authors":"Meihui Luo ,&nbsp;Yuanchun Shan ,&nbsp;Xin Zhang ,&nbsp;Hua Ling ,&nbsp;Li Zhao ,&nbsp;Baoying Huang ,&nbsp;Changcheng Wu ,&nbsp;Ruhan A ,&nbsp;Yao Deng ,&nbsp;Hua Zhao ,&nbsp;Wen Wang ,&nbsp;Jiao Ren ,&nbsp;Fei Ye ,&nbsp;Baisheng Li ,&nbsp;Xianda Yang ,&nbsp;Huijuan Wang ,&nbsp;Weibang Huo ,&nbsp;Yuqian Zhai ,&nbsp;Yize Han ,&nbsp;Houwen Tian ,&nbsp;Wenjie Tan","doi":"10.1016/j.bsheal.2025.03.001","DOIUrl":"10.1016/j.bsheal.2025.03.001","url":null,"abstract":"<div><div>In 2022, a global outbreak of mpox was anticipated, with several cases reported in non-endemic countries in early May. Given the challenge of distinguishing the mpox virus (MPXV) from other pathogens based solely on symptoms, there is an urgent need for prompt and reliable MPXV detection methods. In this study, we developed assays using recombinase-aided amplification (RAA) to identify MPXV and evaluated their applicability with clinical samples. The assays were designed to detect the <em>N4R</em> gene of MPXV. All assays demonstrated detection limits of 1 copy/µL within the reaction system and exhibited no cross-reactivity with ectromelia or the TianTan strain of vaccinia virus, confirming their high specificity. Our established assay provides results in less than 50 min. Furthermore, we evaluated our assay using clinical samples from laboratory-confirmed mpox patients and demonstrated that the RAA-based assay is valuable for diagnosing MPXV infections in field and clinic settings, especially in areas with limited laboratory resources. Overall, three RAA-based nucleic acid assays for MPXV were established, providing a powerful tool for efficient, rapid, and specific detection of MPXV infection.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 2","pages":"Pages 103-109"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the development of infectious clones of human coronaviruses and related applications","authors":"Guanya Liu ,&nbsp;Ruixiao Tan ,&nbsp;Yiyi Wu ,&nbsp;Mengwei Wang ,&nbsp;Baoying Huang ,&nbsp;Wenjie Tan","doi":"10.1016/j.bsheal.2025.01.003","DOIUrl":"10.1016/j.bsheal.2025.01.003","url":null,"abstract":"<div><div>Coronaviruses can infect humans, mammals, and birds, leading to respiratory, gastrointestinal, and neurological diseases. These viruses are significant zoonotic pathogens with nine known types capable of infecting humans. The coronavirus genome, approximately 30 kb in size, is the largest known ribonucleic acid (RNA) virus genome, and its complexity makes assembly and manipulation time-consuming and labor-intensive. Reverse genetic systems are widely used to engineer recombinant viruses that can be adapted at Biosafety Level 2 (BSL-2) for studying viral gene function, replication, pathogenesis, vaccines, and therapeutics. The infectious clones, which enabled the recovery of various viruses after DNA recombinant technology, were indispensable tools for the reverse genetics of viruses. Various techniques for constructing infectious clones of human coronaviruses (HCoV) have been developed, encompassing methods such as vaccinia virus vectors method, <em>in vitro</em> ligation, bacterial artificial chromosome systems, yeast artificial chromosome systems, circular polymerase extension reaction, and the recently reported infectious sub-genomic amplicons technology. This review summarizes the status of various techniques for constructing infectious clones of human coronaviruses and related applications.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 59-73"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-assisted identification of a novel Orthopoxvirus inhibitor targeting F13","authors":"Junwen Luan ,&nbsp;Annan Ming ,&nbsp;Wenbo Zhao ,&nbsp;Liyuan Zhang ,&nbsp;Leiliang Zhang","doi":"10.1016/j.bsheal.2024.12.002","DOIUrl":"10.1016/j.bsheal.2024.12.002","url":null,"abstract":"<div><div>Treatment of mpox virus (MPXV) is crucial for public health. However, research into drugs for MPXV has fallen behind, particularly in anticipation of sudden outbreaks. This study aimed to identify new inhibitors of <em>Orthopoxvirus</em> using artificial intelligence (AI)-assisted methods. We explored AlphaFold v2.0 to simulate the F13 protein structures of MPXV, vaccinia virus (VACV), and variola virus (VARV). Utilizing MOE2019 software, we identified amino acid binding sites suitable for small molecule docking, focusing on a phosphodiesterase active site pocket in F13. Our efforts led to the identification of JCS-2022, a promising new inhibitor that exhibited docking similarities with the known anti-poxvirus drug tecovirimat. <em>In vitro</em> experiments demonstrated that JCS-2022 had a half maximal effective concentration (EC₅₀) of 0.05430 μmol/L (μM), comparable to tecovirimat’s EC₅₀ of 0.04794 μM. At a dosage of 1.6 μM, JCS-2022 significantly reduced VACV plaque size, indicating effective inhibition of extracellular enveloped virus (EEV) formation. Immunofluorescence analysis confirmed a reduction in VACV-induced actin tail formation. Our findings suggest that JCS-2022 is a strong candidate for development as a small molecule inhibitor against <em>Orthopoxvirus</em>, highlighting the potential of AI-assisted methods in accelerating drug discovery for infectious diseases.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 33-37"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational optimization of a pan-coronavirus fusion inhibitory peptide targeting spike’s heptapeptide repeat region","authors":"Peixiang Gao ,&nbsp;Shuo Liu ,&nbsp;Xiaojing Chi ,&nbsp;Xinhui Zhang ,&nbsp;Xiuying Liu ,&nbsp;Xuehua Yang ,&nbsp;Huarui Duan ,&nbsp;Jingya Zhou ,&nbsp;Weijin Huang ,&nbsp;Wei Yang","doi":"10.1016/j.bsheal.2025.01.001","DOIUrl":"10.1016/j.bsheal.2025.01.001","url":null,"abstract":"<div><div>In the past two decades, highly pathogenic coronaviruses (CoVs), such as severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have constituted a grave threat to human health. Broad-spectrum anti-CoV fusion inhibitors that target the heptapeptide repeat (HR) region within the S2 subunit of SARS-CoV-2 spike (S) protein exhibit inhibitory activity against various CoVs. In this study, we employed EK1, a fusion inhibitor previously characterized for its broad spectrum and potent antiviral activity, as a scaffold for computational design to enhance its inhibitory potential using the Rosetta software suite. We designed EK1 variants and synthesized two N-terminally extended EK1 elongation peptides, and evaluated their inhibitory activity. The results revealed that the designed peptides enhanced inhibitory activity against diverse CoVs. Structural analysis and molecular dynamics simulations demonstrated that EK1 variants formed more robust interactions with HR1 of SARS-CoV-2, and these interactions were conserved across different CoVs. These findings underscore the utility of computational approaches in optimizing therapeutic peptides.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 44-58"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring enterovirus pathogenesis and cancer therapy potential through reverse genetics","authors":"Shijin Wang ,&nbsp;Qing Yu ,&nbsp;Junfeng Zhou ,&nbsp;Wanshan Yang ,&nbsp;Yonggang Wang ,&nbsp;Haoran Guo ,&nbsp;Wei Wei","doi":"10.1016/j.bsheal.2025.01.004","DOIUrl":"10.1016/j.bsheal.2025.01.004","url":null,"abstract":"<div><div>Over the past decades, oncolytic viruses have emerged as prominent therapeutic agents with significant potential for anticancer treatment. Enteroviruses (EVs) have garnered particular attention due to their specific tropism for various types of tumor cells. The rapid advancement of reverse genetics has enabled its application in the genetic modification of enteroviruses and the investigation of viral infection mechanisms. The utilization of reverse genetics has significantly enhanced our understanding of the infection mechanisms and pathogenesis of enteroviruses, while concurrently facilitating the development of translational therapies related to these viruses. In this review, we summarize the progress in the application of reverse genetics to oncolytic enteroviruses and their potential clinical applications. Specifically, we discuss the characteristics of EVs and the applications of reverse genetics in EV research. We review the utilization of reverse genetics in mechanistic investigations of EVs and in exploring the oncolytic potential of EVs. Further, we discuss the oncolytic roles of specific EVs including EV-A71, coxsackievirus B3 (CV-B3), echovirus 7 (Echo-7), CV-A21, and poliovirus. Our review highlights the advances in oncolytic therapy utilizing EVs with specific tumor tropism, which holds significant potential for immunotherapy.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 74-82"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characteristics of 12 HBV-I strains in the 2020 national HBV serosurvey in China","authors":"Qiudong Su,&nbsp;Liping Shen,&nbsp;Xiaoqi Guo,&nbsp;Shuang Zhang,&nbsp;Feng Qiu,&nbsp;Shengli Bi,&nbsp;Feng Wang","doi":"10.1016/j.bsheal.2025.01.007","DOIUrl":"10.1016/j.bsheal.2025.01.007","url":null,"abstract":"<div><div>Hepatitis B virus (HBV) is categorized into ten distinct genotypes (A − J), with over 40 subgenotypes identified to date. HBV genotype I (HBV-I), an inter-genotypic recombinant, has emerged during the evolution history of HBV. In this study, we conducted a comprehensive analysis of the genomic characteristics of HBV-I in China, employing a range of methodologies including phylogenetic analysis, nucleotide homology assessment, examination of amino acid substitutions within the PreS/S region, recombination detection, and evolutionary analysis. The 12 HBV-I strains, classified into subgenotype I1 and predominantly serotype <em>adw2</em> (with one exception being <em>ayw1</em>) were preliminarily divided into two clusters based on homology analysis. A higher substitution rate was observed in the antigenic loop of the hepatitis B surface antigen (HBsAg), and the potential immune-escape mutations were found. Molecular clock analysis estimated an average evolutionary rate for HBV-I between 1.17 exp(−4) and 1.61 exp(−4) substitutions/site/year, with the most recent common ancestor traced back to between year 1740 and 1774. The epidemiological surveillance and genomic characterization of HBV genotype I are significant for informing future strategies in the prevention and control of hepatitis B.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 17-25"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological characteristics of human adenovirus infections in China, 2020–2023","authors":"Chongyang Li ,&nbsp;Yitong Lin ,&nbsp;Naiying Mao ,&nbsp;Yixuan Gao ,&nbsp;Ying Liu ,&nbsp;Liwei Sun ,&nbsp;Hui Zhang ,&nbsp;Jin Xu ,&nbsp;Maozhong Li ,&nbsp;Feng Zhang ,&nbsp;Xiaoqing Liu ,&nbsp;Linqing Zhao ,&nbsp;Bing Zhu ,&nbsp;Ye Chen ,&nbsp;Min Mu ,&nbsp;Xiaoling Tian ,&nbsp;Hongmei Xu ,&nbsp;Chaoyang Huang ,&nbsp;Zhong Li ,&nbsp;Jing Xu ,&nbsp;Zhen Zhu","doi":"10.1016/j.bsheal.2025.01.002","DOIUrl":"10.1016/j.bsheal.2025.01.002","url":null,"abstract":"<div><div>To better understand the epidemiological characteristics of human adenovirus (HAdV) infections in China during and after the coronavirus disease 2019 (COVID-19) pandemic, respiratory specimens were collected from 17,562 enrolled patients with acute respiratory infections (ARIs) in 14 sentinel surveillance provinces during 2020–2023. Eight common respiratory viruses were detected using commercially available nucleic acid detection kits. HAdV-positive cases were statistically analyzed for detection rates, geographic distribution, seasonal patterns, demographic characteristics, and co-infection status. The results of this study showed that the overall HAdV detection rate was 5.09 % (894/17,562) during 2020–2023, with a gradual decrease in the annual detection rate from 6.66 % in 2020 to 3.89 % in 2022 and a rebound in 2023 (5.19 %). The overall HAdV detection rate was significantly higher in the southern region (6.15 %) than in the northern region (4.76 %) (<em>P</em> &lt; 0.001). The median age of patients with HAdV infection was 3 years, with infants aged 0–2 years accounting for the majority (41.39 %). HAdV-positive cases were detected throughout the year, with no clear seasonal pattern, and the HAdV epidemic in China during 2020–2023 may have been driven primarily by the virus infection in the southern region. Co-infections were frequent in HAdV-positive cases (overall rate: 36.01 %), primarily consisting of dual infections (79.28 %), with human rhinovirus and human respiratory syncytial virus being the most common coinfecting pathogens. In conclusion, this study suggested the significant regional and temporal variation in HAdV detection rate in China during 2020–2023, and thus ongoing surveillance should be conducted to elucidate the epidemiological dynamics of HAdV infections.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 1","pages":"Pages 26-32"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}