Biosafety and Health最新文献

{"title":"Epidemiology and genetic characterization of tet(X4)-positive Klebsiella pneumoniae and Klebsiella quasipneumoniae isolated from raw meat in Chengdu City, China","authors":"Weishuai Zhai ,&nbsp;Yiqing Wang ,&nbsp;Honghu Sun ,&nbsp;Bo Fu ,&nbsp;Qidi Zhang ,&nbsp;Congming Wu ,&nbsp;Jianzhong Shen ,&nbsp;Dejun Liu ,&nbsp;Yang Wang","doi":"10.1016/j.bsheal.2024.02.004","DOIUrl":"10.1016/j.bsheal.2024.02.004","url":null,"abstract":"<div><p>The rapid spread of mobile tigecycline resistance presents a significant public health threat, particularly with the increasing prevalence of <em>tet</em>(X4)-positive Enterobacterales across various species. This study aimed to investigate the epidemic features and transmission dynamics of <em>tet</em>(X4)-positive <em>Klebsiella pneumoniae</em> (<em>K</em>. <em>pneumoniae</em>) through the analysis of 206 raw meats, including pork (<em>n</em> = 182), beef (<em>n</em> = 16), duck (<em>n</em> = 5), and chicken (<em>n</em> = 3). These samples were collected from schools, markets, and restaurants in Chengdu City, China. A total of 25 isolates were obtained from 13 administrative regions. All isolates exhibited resistance to tetracycline, tigecycline, ampicillin, chloramphenicol, and florfenicol. Over half of the isolates also demonstrated resistance to streptomycin (80 %), sulfamethoxazole/trimethoprim (72 %), ciprofloxacin (64 %), and ampicillin/sulbactam (56 %). Among these strains, 14 distinct sequence types (STs) were identified, revealing evidence of inter-regional clonal spread, notably among 9 <em>K. pneumoniae</em> ST3393. Phylogenetic analysis revealed the presence of two <em>K. pneumoniae</em> ST5 closely resembling hypervirulent <em>K. pneumoniae</em> from Jiangsu. Importantly, 12 isolates were capable of transferring tigecycline resistance to <em>Escherichia coli</em> J53. Further plasmid analysis showed that the <em>tet</em>(X4)-harboring plasmids in <em>K. pneumoniae</em> could be classified into four types, primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid (<em>n</em> = 16) and IncFII plasmid (<em>n</em> = 7), which significantly contributed to the cross-species dissemination of <em>tet</em>(X4). In summary, this study highlights the prevalence of MDR <em>tet</em>(X4)-positive <em>K. pneumoniae</em> in Chengdu, driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer. These findings emphasize the importance of continuous surveillance of <em>tet</em>(X4)-positive <em>K. pneumoniae</em> in raw meat and the implementation of effective measures to control their spread.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 2","pages":"Pages 116-124"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000235/pdfft?md5=20ec39c1f018122af2c5db376c794a33&pid=1-s2.0-S2590053624000235-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140465189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating human exposure to antibiotic resistance genes","authors":"Zhenchao Zhou ,&nbsp;Hong Chen","doi":"10.1016/j.bsheal.2024.02.005","DOIUrl":"10.1016/j.bsheal.2024.02.005","url":null,"abstract":"<div><p>Antibiotic resistance is an escalating global concern, leading to millions of annual fatalities. Antibiotic resistance genes (ARGs) present in bacteria equip them to withstand the effects of antibiotics. Intra- and interspecific ARGs transmission through horizontal gene transfer further exacerbates resistance dissemination. The presence of ARGs in the environment heightens the probability of human exposure via direct inhalation, ingestion, or contact with polluted air, food, or water, posing substantial biosafety and health hazards. Consequently, ARGs represent a critical focal point in public health and environmental safety and are classified as emerging contaminants. This perspective underscores the necessity to assess ARG exposure within the One Health framework and to accord greater attention to the mitigation strategies and tactics associated with ARGs.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 2","pages":"Pages 98-100"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000247/pdfft?md5=ed1bf4fde458d7dea9a3cfcd51d4a15c&pid=1-s2.0-S2590053624000247-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140465198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current epidemiologic features and health dynamics of ESBL-producing Escherichia coli in China","authors":"Sayyed Salman ,&nbsp;Zeeshan Umar ,&nbsp;Yonghong Xiao","doi":"10.1016/j.bsheal.2024.01.002","DOIUrl":"10.1016/j.bsheal.2024.01.002","url":null,"abstract":"<div><p>Extended-spectrum β-lactamase (ESBL)-producing <em>Escherichia coli</em> (<em>E. coli</em>) are widespread in China, with occurrences documented in humans, animals, and the environment. The dissemination of ESBL-producing <em>E. coli</em> is likely facilitated by the widespread use of antibiotics in human and animal agriculture, the presence of antibiotic-resistant bacteria (ARBs) in animal feces, and close human-animal interactions. Plasmids, particularly those belonging to incompatibility (Inc) group, such as IncF, IncI, and IncH families, play a vital role in facilitating the horizontal gene transfer of ESBL genes across various sectors, from humans to animals and the environment. IS<em>26</em> and IS<em>1</em> elements also significantly influences the mobilization and evolution of antibiotic-resistance genes (ARGs), contributing to the spread of ESBL-producing <em>E. coli. bla</em>_CTX-M−14, <em>bla</em>_CTX-15, and <em>bla</em>_CTX-M−55 are prevalent in ESBL-producing <em>E. coli</em> across the three domains and are often found in conjunction with other ARGs. Considering these challenges, it is imperative to take proactive measures to prevent the further spread of ARBs. This includes the judicious and responsible use of antibiotics and efforts to minimize contact with animal feces. Sector-specific strategies should be developed to effectively educate and engage relevant personnel in tackling this multifaceted problem. These efforts are vital to combat the dissemination of ESBL-producing <em>E. coli</em> and preserve public health.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 40-49"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000028/pdfft?md5=bd6904af2835aae7532084858395b44e&pid=1-s2.0-S2590053624000028-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccinia virus viability under different environmental conditions and different disinfectants treatment","authors":"Shan Mei ,&nbsp;Liang Wei ,&nbsp;Yu Xie ,&nbsp;Fei Zhao ,&nbsp;Yu Huang ,&nbsp;Zhangling Fan ,&nbsp;Yamei Hu ,&nbsp;Liming Wang ,&nbsp;Lingwa Wang ,&nbsp;Ying Wang ,&nbsp;Fengwen Xu ,&nbsp;Fei Guo","doi":"10.1016/j.bsheal.2023.12.005","DOIUrl":"10.1016/j.bsheal.2023.12.005","url":null,"abstract":"<div><p>Monkeypox (mpox) outbreak in 2022 has caused more than 91,000 cases, has spread to 115 countries, regions, and territories, and has thus attracted much attention. The stability of poxvirus particles in the environment is recognized as an important factor in determining their transmission. However, few studies have investigated the persistence of poxviruses on material surfaces under various environmental conditions, and their sensitivity to biocides. Here, we systematically measured the stability of vaccinia virus (VACV) under different environmental conditions and sensitivity to inactivation methods via plaque assay, quantitative real-time polymerase chain reaction (qPCR), and Gaussia luciferase (G-luciferase) reporter system. The results show that VACV is stable on the surface of stainless steel, glass, clothing, plastic, towel, A4 paper, and tissue and persists much longer at 4 °C and −20 °C, but is effectively inactivated by ultraviolet (UV) irradiation, heat treatment, and chemical reagents. Our study raises the awareness of long persistence of poxviruses in the environment and provides a simple solution to inactivate poxviruses using common disinfectants, which is expected to help the control and prevention of mpox virus and future poxvirus outbreaks.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 21-27"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001556/pdfft?md5=54dd3bfe0705a2c035a9fdae099789cc&pid=1-s2.0-S2590053623001556-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139194018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and immunoprotective effects of a virus-like particle candidate vaccine of the dominant epidemic D3 genotype coxsackievirus A6 in China","authors":"Xiaoliang Li ,&nbsp;Xizhu Xu ,&nbsp;Jichen Li ,&nbsp;Huanhuan Lu ,&nbsp;Congcong Wang ,&nbsp;Rui Wang ,&nbsp;Jinbo Xiao ,&nbsp;Ying Liu ,&nbsp;Yang Song ,&nbsp;Jingdong Song ,&nbsp;Qiang Sun ,&nbsp;Yong Zhang","doi":"10.1016/j.bsheal.2023.11.001","DOIUrl":"10.1016/j.bsheal.2023.11.001","url":null,"abstract":"<div><p>Coxsackievirus A6 of the D3a genotype (CVA6 D3a) is a primary pathogen causingmainland of China's hand, foot, and mouth disease (HFMD). Viral-like particle (VLP) vaccines represent a potential candidate vaccine to prevent HFMD. This study collected Anti-CVA6 D3a VLPs serum from BALB/c female mice immunized using CVA6 D3a VLPs. The neutralizing antibody levels were compared against the representative 14-JX2018 (D3a) and N4-YN2015 (D3b) strains between the antisera of different immune pathways. The immunoprotective effect of anti-CVA6 D3a VLPs against these strains was monitored using pathological sections and immunohistochemical results of lung and skeletal muscle tissues in seven-day-old Institute of Cancer Research (ICR) mice. Immunological protection against different branches of viruses was evaluated in 7-day-old (serum passive immune protection) and 14-day-old (VLPs active immune protection) neonatal ICR mice models. Serum-neutralizing antibody levels were positively correlated with the number of immunizations and higher against 14-JX2018 than against N4-YN2015. Furthermore, these levels were significantly higher with abdominal injection than intramuscular injection. The immunized serum of 7-day-old ICR mice inoculated three times was 100 % protected against CVA6 D3a 14-JX2018 (lethal titer: 10^6.25 TCID₅₀) and CVA6 D3b N4-YN2015 (lethal titer: 10^5.25TCID₅₀) fatal attacks, respectively. For ICR mice that have completed two active immunizations for 14 days, both CVA6 D3a 14-JX2015 (challenge titer: 10^8.25 TCID₅₀) and CVA6 D3b N4-YN2015 (challenge titer: 10^7.25 TCID₅₀) were used for the challenge, and the mice were able to survive. Overall, the CVA6 D3a VLPs prepared in this study are a potential vaccine candidate for CVA6, as it has the optimal protective effect against both CVA6 D3a and D3b evolutionary branches viruses.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 28-34"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001301/pdfft?md5=d73fb1c5fc096cd13631fe46e9e53c53&pid=1-s2.0-S2590053623001301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progressing our knowledge of enterovirus: Epidemiology, diagnosis, prevention, control, and beyond","authors":"Jing Lu ,&nbsp;Zexin Tao ,&nbsp;Yong Zhang","doi":"10.1016/j.bsheal.2024.01.001","DOIUrl":"10.1016/j.bsheal.2024.01.001","url":null,"abstract":"","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 3-4"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053624000016/pdfft?md5=a5b83533dfac6870ac78217f61094e71&pid=1-s2.0-S2590053624000016-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139454406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and identifying natural products with SARS-CoV-2 infection inhibitory activity from medicinal fungi","authors":"Shuang Zhao ,&nbsp;Amelia Siqi Huang ,&nbsp;Weibo Zhang ,&nbsp;Lili Ren ,&nbsp;Hexiang Wang ,&nbsp;Jianbin Wang ,&nbsp;Xinyang Shao ,&nbsp;Guanbo Wang","doi":"10.1016/j.bsheal.2023.12.006","DOIUrl":"10.1016/j.bsheal.2023.12.006","url":null,"abstract":"<div><p>The coronavirus disease of 2019 (COVID-19), a global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), can result in severe health complications. In addition to physical preventative measures, pharmaceutical intervention is also crucial. Numerous natural products from medicinal fungi have shown promise as potential antiviral drugs and may serve as a source of effective components with antiviral activity against SARS-CoV-2 and other coronaviruses. In this study, we developed a workflow that integrates viral infection inhibition assays at both cellular and molecular levels, as well as molecular separation and characterization, to screen and identify natural products with antiviral activity. Using this workflow, we screened 167 extracts extracted from 36 medicinal fungi using optimized extraction methods. We assessed the antiviral effects of these extracts by measuring their ability to inhibit SARS-CoV-2 infection and receptor binding domain - human angiotensin-converting enzyme 2 (RBD-hACE2) binding <em>in vitro</em>. Following charge- and size-based characterization of the active compounds through filtration and chromatographic fractionation, mass spectrometry characterization of the fractionated compounds revealed that the active components are polysaccharides and determined their monosaccharide residue composition. Our findings provide new insights into the antiviral potential of natural products and their screening strategies and may contribute to the development of effective antiviral therapeutics against COVID-19 and other diseases.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 12-20"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001568/pdfft?md5=877e3eb206dcad46e6c0cde9dc7a3818&pid=1-s2.0-S2590053623001568-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “HLA-E-restricted Hantaan virus-specific CD8+ T cell responses enhance the control of infection in hemorrhagic fever with renal syndrome” [Biosaf. Health 5 (2023) 289–299]","authors":"Kang Tang ,&nbsp;Yusi Zhang ,&nbsp;Xinyu Li ,&nbsp;Chunmei Zhang ,&nbsp;Xiaozhou Jia ,&nbsp;Haifeng Hu ,&nbsp;Lihua Chen ,&nbsp;Ran Zhuang ,&nbsp;Yun Zhang ,&nbsp;Boquan Jin ,&nbsp;Ying Ma","doi":"10.1016/j.bsheal.2023.11.002","DOIUrl":"10.1016/j.bsheal.2023.11.002","url":null,"abstract":"","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Page 59"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001507/pdfft?md5=c0dcd5db4375dcbbe8ade92ad85ccf5a&pid=1-s2.0-S2590053623001507-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Launch of the transdisciplinary health science journal hLife","authors":"Qun Yan ,&nbsp;Kieran Gary Thow","doi":"10.1016/j.bsheal.2023.12.001","DOIUrl":"10.1016/j.bsheal.2023.12.001","url":null,"abstract":"","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 1-2"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001519/pdfft?md5=2077617f0fbca8ece0bd02d93c6205df&pid=1-s2.0-S2590053623001519-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139014145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance containment in Africa: Moving beyond surveillance","authors":"Zikora Kizito Glory Anyaegbunam ,&nbsp;Ifeanyi Elibe Mba ,&nbsp;Yandev Doowuese ,&nbsp;Ngozi J. Anyaegbunam ,&nbsp;Toluwalase Mba ,&nbsp;Fetuata Aminat Aina ,&nbsp;Vincent Nnamdigadi Chigor ,&nbsp;Emeka Innocent Nweze ,&nbsp;Emmanuel A. Eze","doi":"10.1016/j.bsheal.2023.12.003","DOIUrl":"10.1016/j.bsheal.2023.12.003","url":null,"abstract":"<div><p>Worldwide, infections caused by drug-resistant pathogens constitute a significant challenge threatening therapeutic efforts. According to the World Health Organization (WHO), antimicrobial resistance (AMR) ranks among the top 10 global public health threats. Organisms with a high rate of multiple host adaptivity, significant genetic diversity (multiple lineages), high virulence factors, and genetic exchange have been isolated from various sources (humans, animals, and the environment) even without exposure to prior antibiotics. Till now, the source of AMR and how resistant clones are selected in the environment remain largely elusive, and potential anthropogenic transmission has been reported in different studies. Various drug-resistant pathogens, lineages, resistant clones, outbreak clusters, plasmid replicates, and genes that play a critical role in resistance dissemination have been identified. Maintenance of certain multidrug-resistant (MDR) determinants has also been shown to enhance or support the propagation of MDR. So far, significant advances have been made in understanding the burden of AMR. However, overcoming AMR requires a holistic approach, as there is no single approach with sufficient precision to curb the threat. While strengthening AMR surveillance efforts is essential, as we have shown, there is also a need to intensify efforts to strengthen therapeutic interventions, especially in priority regions such as Africa. Herein, we discussed the burden of AMR and the dissemination of AMR in humans, animals, and the environment (non-medical drivers). We further delved into the big questions on Africa and discussed how therapeutic interventions involving vaccines and other viable biomaterials could be pivotal in reducing the burden of AMR to the barest minimum.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 50-58"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001532/pdfft?md5=6c97c2651afb1db4e3276a7909e28a99&pid=1-s2.0-S2590053623001532-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}