Biosafety and Health最新文献_第5页

Efficacy of SARS-CoV-2 detection from used surgical masks compared with standard detection method 从使用过的外科口罩中检测 SARS-COV-2 的效果与标准检测方法的比较

Biosafety and Health Pub Date : 2024-02-01 DOI: 10.1016/j.bsheal.2023.12.004

Uraporn Phumisantiphong , Anan Manomaipiboon , Yuttana Apichatbutr , Kittisak Pholtawornkulchai , Chunlanee Sangketchon , Busaba Supawattanabodee , Thananda Trakarnvanich

{"title":"Efficacy of SARS-CoV-2 detection from used surgical masks compared with standard detection method","authors":"Uraporn Phumisantiphong , Anan Manomaipiboon , Yuttana Apichatbutr , Kittisak Pholtawornkulchai , Chunlanee Sangketchon , Busaba Supawattanabodee , Thananda Trakarnvanich","doi":"10.1016/j.bsheal.2023.12.004","DOIUrl":"10.1016/j.bsheal.2023.12.004","url":null,"abstract":"<div><p>The real-time reverse transcription-polymerase chain reaction (RT-PCR) test is the gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. Proper specimen collection and obtaining a sufficient specimen are the most essential steps for laboratory diagnosis. The nasopharyngeal (NP) swab is recommended as the reference collection method. However, NP swab collection is invasive and uncomfortable for patients and poses some risk to healthcare workers. This study aimed to compare the efficacy of SARS-CoV-2 RNA detection from surgical masks with the NP swab method using RT-PCR testing. Of 269 patients, RT-PCR RNA from NP swabs was detected among 82 patients (30.5%) and was undetected among 187 patients (69.5%). All patients were tested for SARS-CoV-2 RNA from surgical masks. SARS-CoV-2 RNA was detected in 25/82 (30.5%) surgical mask filters, while undetected among 57 (69.5%). For the surgical mask with an average use time of 7.05 h, the sensitivity was 30.5%, the specificity was 100.0%, with positive predictive value of 100.0% and negative predictive value of 76.2%. Therefore, surgical masks could be an alternative non-invasive specimen source for SARS-CoV-2 RT-PCR testing. The results of our study suggest that the test could be employed after wearing surgical masks for at least 8-12 h, with increased sensitivity when used for more than 12 h.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 35-39"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001544/pdfft?md5=4f3ea2cfc201bb2d805f6d26766e1f60&pid=1-s2.0-S2590053623001544-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More common RNAemia in the early stage of severe SARS-CoV-2 BF.7.14 infections in pediatric patients 在儿科严重 SARS-CoV-2 BF.7.14 感染的早期阶段，RNA 血症更为常见

Biosafety and Health Pub Date : 2024-02-01 DOI: 10.1016/j.bsheal.2023.12.002

Yu Sun , Runan Zhu , Yang Pan , Ri De , Shuang Liu , Liping Jia , Bing Lv , Xiaoyun Li , Dongmei Chen , Yao Yao , Dong Qu , Daitao Zhang , Linqing Zhao

{"title":"More common RNAemia in the early stage of severe SARS-CoV-2 BF.7.14 infections in pediatric patients","authors":"Yu Sun , Runan Zhu , Yang Pan , Ri De , Shuang Liu , Liping Jia , Bing Lv , Xiaoyun Li , Dongmei Chen , Yao Yao , Dong Qu , Daitao Zhang , Linqing Zhao","doi":"10.1016/j.bsheal.2023.12.002","DOIUrl":"10.1016/j.bsheal.2023.12.002","url":null,"abstract":"<div><p>The risk factors of severe infections in children during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak in Beijing remain elusive. SARS-CoV-2-positive children admitted to the intensive care unit (ICU) with collected plasma specimens were enrolled and screened for common pathogens using capillary electrophoresis-based multiplex PCR from December 12, 2022, to January 24, 2023. The SARS-CoV-2 sub-variants were identified using next-generation sequencing. Plasma was positive for two (positive; P), one (suspicious; S), or no (negative; N) SARS-CoV-2 genes were classified as plasmatic RNA-positive (RNAemia; P + S) or without RNAemia (N). Clinical and laboratory data of the enrolled cases were then collected and analyzed. The 34 enrolled children included 26 males and 24 younger than three years. All were negative for other respiratory pathogens. BF.7.14 (18/29) was the predominant subvariant. Viral loads in respiratory specimens, hours from symptom onset to the first respiratory specimen collection (time-variable), with comorbidities and BF.7.14 and BA.5.2 distributions were significantly different in P vs. N and RNAemia vs. without RNAemia group. Among most cases, the T lymphocyte ratios decreased, while the cytokine level and the B lymphocyte ratio increased. The time variables were 2.22 ± 2.05 and 4.00 ± 2.49 days in BF.7.14 and BA.5.2 infections, respectively. In conclusion, SARS-CoV-2 was more likely to cause severe infections among males aged ≤ 3 years old with comorbidities during the SARS-CoV-2 outbreak in Beijing, while RNAemia is more common in children at the early stage of severe BF.7.14 infections, and most had high cytokine levels and B-cell activation.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"6 1","pages":"Pages 5-11"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001520/pdfft?md5=ba81f2f260178184e2a58320e62be649&pid=1-s2.0-S2590053623001520-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaceutical effluent evokes superbugs in the environment: A call to action 制药废水唤起环境中的超级细菌行动呼吁

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.10.005

Rehab A. Rayan

{"title":"Pharmaceutical effluent evokes superbugs in the environment: A call to action","authors":"Rehab A. Rayan","doi":"10.1016/j.bsheal.2023.10.005","DOIUrl":"10.1016/j.bsheal.2023.10.005","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is a growing global threat, especially in low- and middle-income countries (LMICs), causing prolonged illnesses, heightened antimicrobial use, increased healthcare expenses, and avoidable deaths. If not tackled, AMR could force 24 million people into severe poverty by 2030 and hinder progress on Sustainable Development Goals (SDGs). AMR spreads through interconnected ecosystems, with humans, animals, and the environment serving as reservoirs. Pharmaceutical wastewater, loaded with antibiotics and resistance genes, poses a significant environmental risk, mainly due to inadequate treatment and irresponsible disposal. The pharmaceutical industry is a notable contributor to environmental antibiotic pollution, with varying effluent management practices. Contaminated pharmaceutical wastewater discharge harms water sources and ecosystems. Urgent collaborative efforts are needed across policymakers, regulators, manufacturers, researchers, civil society, and communities, adopting a One Health approach to curb AMR's spread. Developing global standards for pharmaceutical effluent antibiotic residues, effective treatment methods, and improved diagnostics are vital in addressing AMR's environmental impact while safeguarding public health and the environment. National action plans should encompass comprehensive strategies to combat AMR. Preserving antibiotic efficacy and ensuring sustainable production require a united front from all stakeholders.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 363-371"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001295/pdfft?md5=a6e45a4472199af2e4256dcedb217c5a&pid=1-s2.0-S2590053623001295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare peptide anchors of HLA class I alleles contribute to the COVID-19 disease severity and T cell memory HLA I类等位基因的稀有肽锚有助于COVID-19疾病的严重程度和T细胞记忆

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.09.002

Xin Wang , Jie Zhang , Peipei Guo , Yuanyuan Guo , Xiaonan Yang , Maoshun Liu , Danni Zhang , Yaxin Guo , Jianbo Zhan , Kun Cai , Jikun Zhou , Shaobo Dong , Jun Liu

{"title":"Rare peptide anchors of HLA class I alleles contribute to the COVID-19 disease severity and T cell memory","authors":"Xin Wang , Jie Zhang , Peipei Guo , Yuanyuan Guo , Xiaonan Yang , Maoshun Liu , Danni Zhang , Yaxin Guo , Jianbo Zhan , Kun Cai , Jikun Zhou , Shaobo Dong , Jun Liu","doi":"10.1016/j.bsheal.2023.09.002","DOIUrl":"10.1016/j.bsheal.2023.09.002","url":null,"abstract":"<div><p>Understanding how human leukocyte antigen (HLA) polymorphism affects both the susceptibility and severity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection will help to identify individuals at higher risk to better manage and prioritize vaccination at the clinical level and explain the differences in epidemic trends in different regions at the epidemiological level. This study compared the frequencies of HLA class I alleles (HLA-A, B) in 214 coronavirus disease 2019 (COVID-19) patients with different disease severity and 35 healthy controls and analyzed the correlations between specific HLA alleles and disease severity and T cell memory. The results showed no significant difference in HLA allele frequencies between COVID-19 patients and healthy controls (<em>P</em> > 0.05). The allele HLA-B*13:02 was significantly correlated with the disease severity of COVID-19 patients (<em>P</em> = 0.006). After adjustment for age and disease severity, the T cell responses of COVID-19 convalescents with the allele HLA-B*40:01 may be lower at six months (<em>P</em> = 0.044) and 12 months (<em>P</em> = 0.069). Moreover, these results may be due to their rare peptide anchors by analyzing the binding peptide motifs of these HLA alleles. The study may be valuable for investigating the potential association of specific HLA alleles with SARS-CoV-2 infection.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 355-362"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259005362300109X/pdfft?md5=772a854789b56337fe2f308588d54946&pid=1-s2.0-S259005362300109X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135304776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on enteroviral protease 2A: Structure, function, and host factor interaction 肠道病毒蛋白酶 2A 的最新进展：结构、功能和宿主因子相互作用

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.09.001

Ying Liu, Jichen Li, Yong Zhang

{"title":"Update on enteroviral protease 2A: Structure, function, and host factor interaction","authors":"Ying Liu, Jichen Li, Yong Zhang","doi":"10.1016/j.bsheal.2023.09.001","DOIUrl":"10.1016/j.bsheal.2023.09.001","url":null,"abstract":"<div><p>Enteroviruses (EVs) are human pathogens commonly observed in children aged 0–5 years and adults. EV infections usually cause the common cold and hand-foot-and-mouth disease; however, more severe infections can result in multiorgan complications, such as polio, aseptic meningitis, and myocarditis. The molecular mechanisms by which enteroviruses cause these diseases are still poorly understood, but accumulating evidence points to two enterovirus proteases, 2A<sup>pro</sup> and 3C<sup>pro</sup>, as the key players in pathogenesis. The 2A<sup>pro</sup> performs post-translational proteolytic processing of viral polyproteins and cleaves several host factors to evade antiviral immune responses and promote viral replication. It was also discovered that coxsackievirus-induced cardiomyopathy was caused by 2A<sup>pro</sup>-mediated cleavage of dystrophin in cardiomyocytes, indicating that cellular protein proteolysis may play a key role in enterovirus-associated diseases. Therefore, studies of 2A<sup>pro</sup> could reveal additional substrates that may be associated with specific diseases. Here, we discuss the genetic and structural properties of 2A<sup>pro</sup> and review how the protease antagonizes innate immune responses to promote viral replication, as well as novel substrates and mechanisms for 2A<sup>pro</sup>. We also summarize the current approaches for identifying the substrates of 2A<sup>pro</sup> to discover novel mechanisms relating to certain diseases.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 331-338"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001088/pdfft?md5=b1193fa7bfc25dfea6755bc90a652259&pid=1-s2.0-S2590053623001088-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135248591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability and infectivity of enteroviruses on dry surfaces: Potential for indirect transmission control 肠道病毒在干燥表面的稳定性和感染性：间接传播控制的潜力

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.07.001

Pichamon Sittikul , Pimolpachr Sriburin , Jittraporn Rattanamahaphoom , Warisa Nuprasert , Nipa Thammasonthijarern , Janjira Thaipadungpanit , Weerawan Hattasingh , Nathamon Kosoltanapiwat , Pilaipan Puthavathana , Supawat Chatchen

{"title":"Stability and infectivity of enteroviruses on dry surfaces: Potential for indirect transmission control","authors":"Pichamon Sittikul , Pimolpachr Sriburin , Jittraporn Rattanamahaphoom , Warisa Nuprasert , Nipa Thammasonthijarern , Janjira Thaipadungpanit , Weerawan Hattasingh , Nathamon Kosoltanapiwat , Pilaipan Puthavathana , Supawat Chatchen","doi":"10.1016/j.bsheal.2023.07.001","DOIUrl":"10.1016/j.bsheal.2023.07.001","url":null,"abstract":"<div><p>Hand, foot, and mouth disease (HFMD) is a contagious disease mainly occurring in young children, and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand. Moreover, the World Health Organization (WHO) monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System. HFMD is mainly caused by a group of enteroviruses (EVs) transmitted through direct contact (person to person) and indirect contact with contaminated objects (surface-to-hand). However, few studies have examined the surface stability of EVs. In this study, we investigated the stability of enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) on three different dry surfaces (wood, plastic, and stainless steel) using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) staining of viable cells and real-time polymerase chain reaction (viral genome detection). The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces. However, viral RNA could be detected on dry surfaces for up to 28 days. Concerning heat inactivation, both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min. Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 339-345"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623000794/pdfft?md5=aac47793878925a98a1ea15c3c47d054&pid=1-s2.0-S2590053623000794-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43001420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and characteristics of identified early mpox cases in Guangdong Province, China: Implications for prevention and control 中国广东省已发现的早期天花病例的流行病学和特征：对预防和控制的影响

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.10.003

Yinan Zong , Yuwei Yang , Dongfeng Kong , Jianxiong Xu , Zimian Liang , Fengxiang Shi , Jianhua Huang , Min Kang , Haojie Zhong , Wenjia Liang , Yan Li

{"title":"Epidemiology and characteristics of identified early mpox cases in Guangdong Province, China: Implications for prevention and control","authors":"Yinan Zong , Yuwei Yang , Dongfeng Kong , Jianxiong Xu , Zimian Liang , Fengxiang Shi , Jianhua Huang , Min Kang , Haojie Zhong , Wenjia Liang , Yan Li","doi":"10.1016/j.bsheal.2023.10.003","DOIUrl":"10.1016/j.bsheal.2023.10.003","url":null,"abstract":"<div><p>In June 2023, the 2022 global mpox (monkeypox) outbreak began to affect Guangdong Province, one of the first regions in mainland China to report mpox cases. By July 10, 2023, 93 mpox cases had been reported in Guangdong Province. This study describes the epidemiological characteristics of these patients by collecting and analyzing data on demographics, sexual behavior, medical history, travel history, clinical symptoms, and diagnostic pathways and processes. The 93 mpox cases were all men aged 20-48 years, 95.70 % were men who have sex with men (MSM), and 48.39 % were HIV-positive. A few cases were married (to women) or living with children. The rash was present in almost all cases (98.91 %), with the most common sites being the genital and perianal areas (70.33 %). Intimate sexual contact was suspected to be the main route of infection, with a median incubation period of 8.5 days (IQR 5.0–11.8). The number of cases increased rapidly, and most patients had no history of international travel, suggesting sustained community transmission within the MSM population in Guangdong Province. In addition, 93.55 % of cases were detected by medical institutions, and more than half of these patients had two or more hospital visits before being diagnosed as an mpox case, indicating that clinicians need further training to increase their sensitivity to mpox. Targeted interventions should prioritize MSM while remaining vigilant for transmission to other populations, such as women and children.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 321-325"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001271/pdfft?md5=2729ab8e1839de24cdaf3889a869379d&pid=1-s2.0-S2590053623001271-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135850263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China 从中国宁夏分离的人类埃可病毒 30 的遗传特征和分子流行病学研究

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.10.001

Fang Yuan , Xinfeng Wei , Xueping Ma , Jiangtao Ma , Xuemin Ma , Xiaoqiang Sun , Min Cao , Juan Zhou , Wei Zhang , Hui Chen , Rui Wang , Jichen Li , Qiang Sun

{"title":"Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China","authors":"Fang Yuan , Xinfeng Wei , Xueping Ma , Jiangtao Ma , Xuemin Ma , Xiaoqiang Sun , Min Cao , Juan Zhou , Wei Zhang , Hui Chen , Rui Wang , Jichen Li , Qiang Sun","doi":"10.1016/j.bsheal.2023.10.001","DOIUrl":"10.1016/j.bsheal.2023.10.001","url":null,"abstract":"<div><p>In October 2017, a small outbreak of echovirus 30 (E30) associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region. That year, we observed a significant increase in E30 levels in an acute flaccid paralysis (AFP) case surveillance system. To investigate their phylogenetic relationships, we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases, AFP cases, and healthy children. We found that the E30 strains circulating in Ningxia belong to two lineages (H and J). The strains isolated in 2010, 2012, and 2016 belonged to the H lineage. In 2017, a new lineage, J, emerged as the dominant lineage. Phylogenetic trees were constructed based on the whole genome and <em>P1</em>, <em>P2</em>, and <em>P3</em> regions; clustering with other types of enterovirus species B was found, suggesting that recombination events had occurred. The recombination sites were mainly in regions <em>2B</em>, <em>2C</em>, and <em>3D</em>. This study confirmed that the E30 strains in Ningxia in 2010, 2012, and 2016 had different recombination patterns and were recombined with different enteroviruses. The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 346-354"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623001258/pdfft?md5=c275c9dd17af77cbf0078b6df70b3c70&pid=1-s2.0-S2590053623001258-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135963649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and application of national reference panels for SARS-CoV-2 antigen detection kit 建立和应用国家 SARS-CoV-2 抗原检测试剂盒参考面板

Biosafety and Health Pub Date : 2023-12-01 DOI: 10.1016/j.bsheal.2023.10.002

Tingting Ma , Donglai Liu , Keliang Lyu , Tingting Gao , Dawei Shi , Lanqing Zhao , Shu Shen , Yabin Tian , Sihong Xu , Haiwei Zhou

{"title":"Establishment and application of national reference panels for SARS-CoV-2 antigen detection kit","authors":"Tingting Ma , Donglai Liu , Keliang Lyu , Tingting Gao , Dawei Shi , Lanqing Zhao , Shu Shen , Yabin Tian , Sihong Xu , Haiwei Zhou","doi":"10.1016/j.bsheal.2023.10.002","DOIUrl":"10.1016/j.bsheal.2023.10.002","url":null,"abstract":"<div><p>To develop a national reference panel for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen detection kit and establish a quality standard. The cultures of SARS-CoV-2 and other pathogens were collected to establish a national reference panel for SARS-CoV-2 antigen detection. The stability and homogeneity of the reference panel were evaluated. Based on World Health Organization (WHO) guidance and nucleic acid quantitative results, a quality standard reference panel was established. Currently, three generations of SARS-CoV-2 antigen national reference materials with batch numbers 370095–202001, 370095–202202, and 370095–202203 have been successfully established. These national reference panels comprised 8 positive samples, 20 negative samples, 1 repetitive sample, and 1 lower detection limit sample. The stability and homogeneity of the reference panel meet the requirements. The quality standards are as follows: the positive and negative coincidence rates are 8/8 and 20/20, respectively. The 10 test results of the medium and low-concentration repetitive reference materials should be positive, and the color rendering should be uniform (or the coefficient of variance should not be higher than 20.0%). The lower detection limit should be at least 5 × 10<sup>5</sup> U/mL (equivalent to copies/mL), and higher concentrations above the lower detection limit must be positive. A national reference panel for the SARS-CoV-2 antigen detection kit has been established. As the standard of SARS-CoV-2 antigen reagents, the reference panel has played a crucial role in the pre-marketing quality evaluation and post-marketing quality supervision in China.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 326-330"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259005362300126X/pdfft?md5=d9245d6267bc792587bef77303b11076&pid=1-s2.0-S259005362300126X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136009811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in treatment strategies for COVID-19: Insights from other coronavirus diseases and prospects 新冠肺炎治疗策略的进展：来自其他冠状病毒疾病的见解和前景

Biosafety and Health Pub Date : 2023-10-01 DOI: 10.1016/j.bsheal.2023.08.003

Yingwen Li , Jiaming Lan , Gary Wong

引用次数: 0