Biosafety and Health最新文献

{"title":"HLA-E-restricted Hantaan virus-specific CD8+ T cell responses enhance the control of infection in hemorrhagic fever with renal syndrome","authors":"Kang Tang ,&nbsp;Yusi Zhang ,&nbsp;Xinyu Li ,&nbsp;Chunmei Zhang ,&nbsp;Xiaozhou Jia ,&nbsp;Haifeng Hu ,&nbsp;Lihua Chen ,&nbsp;Ran Zhuang ,&nbsp;Yun Zhang ,&nbsp;Boquan Jin ,&nbsp;Ying Ma","doi":"10.1016/j.bsheal.2023.06.002","DOIUrl":"10.1016/j.bsheal.2023.06.002","url":null,"abstract":"<div><p>Infection with the Hantaan virus (HTNV) may result in severe hemorrhagic fever with renal syndrome (HFRS). The functions of HLA-E-restricted CD8⁺ T lymphocytes in virus control and vaccine development have recently received increased attention. The purpose of this research is to discover HLA-E-restricted CD8⁺ T cell epitopes on HTNV as well as the features of these epitope-specific CD8⁺ T cells in HFRS patients. To anticipate HLA-E-restricted HTNV epitopes, the NetMHCpan servers were utilized. The K562/HLA-E cell binding test and the enzyme-linked immunospot assay were used to confirm epitope binding to HLA-E. The number and features of HLA-E-restricted epitope-specific CD8⁺ T lymphocytes in HFRS patients were investigated using tetramer staining, intracellular cytokine labeling, proliferation, and cytotoxicity assays. Six HTNV-derived HLA-E-restricted CD8⁺ T cell epitopes were found in this study. In mild/moderate HFRS patients, the frequency of HLA-E-restricted epitope-specific CD8⁺ T cells was greater than in severe/critical patients. CD38⁺HLA-DR⁺ HLA-E-restricted CD8⁺ T cells were identified. Meanwhile, CD45RA⁺CCR7⁻ effector memory-re-expressing CD45RA T cells with early and intermediate maturation and differentiation characteristics predominated. Notably, CD8⁺ T cells from milder HFRS patients produced more interferon-γ, interleukin-2, and granzyme B, had a stronger proliferative potential, and were inversely linked with the amount of plasma HTNV virus load. Furthermore, HLA-E-restricted epitope-specific CD8⁺ T cells demonstrated improved cytotoxic activity <em>in vitro</em> during the acute stage of HFRS. Taken together, the findings demonstrate the protective effects of HLA-E-restricted CD8⁺ T cells during HTNV infection, suggesting that HLA-E-targeted vaccines against HTNV might be developed for HLA-diverse populations.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 289-299"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42942877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular phylogenomic analysis reveals a single origin of monkeypox virus transmission in Guangzhou, China in June 2023","authors":"Mengling Jiang ,&nbsp;Xizi Deng ,&nbsp;Qinghong Fan ,&nbsp;Fei Gu ,&nbsp;Huiqin Yang ,&nbsp;Jian Wang ,&nbsp;Xiaoping Tang ,&nbsp;Fengyu Hu ,&nbsp;Yun Lan ,&nbsp;Feng Li","doi":"10.1016/j.bsheal.2023.08.002","DOIUrl":"10.1016/j.bsheal.2023.08.002","url":null,"abstract":"<div><p>The monkeypox (mpox) virus has caused worldwide transmission since its initial report in England in early May 2022. Available data from the World Health Organization (WHO) show that Europe and the Americas experienced a huge wave of mpox virus infection. Now the number of infected cases is on the rise in Asia. Several sporadic infections have been reported in China. In this study, we obtained high-quality whole viral genomic sequences using a mpox virus-specific amplicon-based sequencing strategy. Our analysis of the phylogenomic characteristics indicated that all eight mpox virus sequences from Guangzhou belonged to the clade IIb lineage B.1.3 cluster. However, we could not locate the exact origins where the virus was imported, based on all the available mpox virus sequences from the Global Initiative on Sharing Avian Influenza Data (GISAID) database (<span>https://gisaid.org/</span><svg><path></path></svg>), except for their closest sequence similarity to that was reported from Japan. Novel amino acid mutations were found among the eight cases, suggesting that a local transmission may have occurred in Guangzhou, China.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 255-258"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42620361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation into the epidemiology, genetic characteristics, and clinical manifestations of the first monkeypox outbreak in Shenzhen, China","authors":"Jia Wan ,&nbsp;Xiaomin Zhang ,&nbsp;Jing Qu ,&nbsp;Bo Peng ,&nbsp;Dongfeng Kong ,&nbsp;Jianhua Lu ,&nbsp;Qinghua Hu ,&nbsp;Zhifeng Zhou ,&nbsp;Haiduan Lin ,&nbsp;Xiangjie Yao ,&nbsp;Yulin Fu ,&nbsp;Qing Xu ,&nbsp;Ying Lin ,&nbsp;Yan Yang ,&nbsp;Jinzhen Tang ,&nbsp;Lin Lin ,&nbsp;Huimin Li ,&nbsp;Ziquan Lv ,&nbsp;Zhen Zhang ,&nbsp;Xuan Zou ,&nbsp;Xiaolu Shi","doi":"10.1016/j.bsheal.2023.08.001","DOIUrl":"10.1016/j.bsheal.2023.08.001","url":null,"abstract":"<div><p>This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen, China, encompassing clinical symptomatology, therapeutic approaches, epidemiological research, and comprehensive laboratory tests, aiming to establish a robust reference for future monkeypox mitigation and management strategies. The investigation involved a thorough investigation of all identified positive cases, including extensive molecular analysis by nucleic acid detection and whole-genome sequencing of the monkeypox virus. Contact tracing and containment of the infected individuals were also undertaken. Three distinct monkeypox cases were identified in this unique outbreak, exhibiting mild and atypical clinical manifestations, primarily fever and rash. All cases were associated with a single transmission chain, primarily facilitated through close contact and homosexual behavior, indicative of a high-risk factor for monkeypox transmission.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 259-265"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43725974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral rebound and safety of nirmatrelvir/ritonavir for lung-transplant recipients infected with SARS-CoV-2","authors":"Hui Li ,&nbsp;Li Zhao ,&nbsp;Ke Huang ,&nbsp;Xiaoxing Wang ,&nbsp;Fei Zhou ,&nbsp;Yiming Feng ,&nbsp;Liang Ma ,&nbsp;Bin Cao ,&nbsp;Wenhui Chen","doi":"10.1016/j.bsheal.2023.08.004","DOIUrl":"10.1016/j.bsheal.2023.08.004","url":null,"abstract":"<div><p>Data on the viral rebound and safety of nirmatrelvir/ritonavir in lung transplant (LTx) recipients are limited. The study prospectively followed four LTx recipients. Clinical characteristics, viral RNA dynamic in throat swabs, and tacrolimus blood concentration were monitored regularly. All four LTx recipients, aged 35–74 years, were not vaccinated against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). They got coronavirus disease 2019 (COVID-19) after more than one week of admission during the era of Omicron. All cases received nirmatrelvir/ritonavir (NM/r) within two days of infection, and the relative viral RNA copies dropped quickly. Viral load rebound was observed in all four cases after discontinuation of the first five days of NM/r treatment. Three of them received another 5-days antiviral therapy with NM/r. The duration of positive viral PCR testing was 25-28 days. None of them progressed into severe or critical COVID-19. Tacrolimus was stopped 12 h before NM/r and held during the 5-day course of antiviral therapy. Blood concentration of tacrolimus were maintained at a baseline level during these five days. Tacrolimus was re‐initiated at its baseline daily dose 3-4 days after NM/r therapy. However, during the second round of antiviral therapy with NM/r, the concentration of tacrolimus fluctuated wildly. In conclusion, the 5-day course of NM/r treatment was not sufficient for LTx recipients and the viral rebound was common. More data are needed to clarify whether LTx recipients with SARS-CoV-2 viral rebound could benefit from additional treatment with NM/r.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 266-271"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43864855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs","authors":"Zhiyuan Gong ,&nbsp;Hailun Li ,&nbsp;Meichen Qian ,&nbsp;Yujie Bai ,&nbsp;Hongli Jin ,&nbsp;Jingxuan Sun ,&nbsp;Mengyao Zhang ,&nbsp;Cuicui Jiao ,&nbsp;Pei Huang ,&nbsp;Yuanyuan Li ,&nbsp;Haili Zhang ,&nbsp;Hualei Wang","doi":"10.1016/j.bsheal.2023.05.005","DOIUrl":"10.1016/j.bsheal.2023.05.005","url":null,"abstract":"<div><p>Although inactivated vaccines against rabies have the advantage of high safety, effective protection against rabies virus (RABV) infection often requires multiple, high-dose immunization. Incorporating a molecular adjuvant into the viral particles has been found to be a useful strategy to promote the immune effectiveness of inactivated vaccines. In this study, we constructed a recombinant virus, rCVS11-LTB, which chimerically expresses a molecular adjuvant heat-labile enterotoxin B subunit (LTB) protein on the surface of the RABV particles. Immunogenicity <em>in vivo</em> was found to be promoted by rCVS11-LTB through the activation of dendritic cells (DCs). Our results demonstrated that inactivated rCVS11-LTB was able to induce higher levels of virus-neutralizing antibodies (VNAs) in both mice and dogs than the parent virus rCVS11, to enhance the cellular immune response and T cell immune memory in mice, and was also able to provide 100% protection in mice from lethal doses of rabies virus, indicating its potential as a safe and effective inactivated rabies vaccine candidate.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 308-319"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48445393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The recombinant truncated envelope protein of West Nile virus adjuvanted with Alum/CpG induces potent humoral and T cell immunity in mice","authors":"Yongping Du ,&nbsp;Yao Deng ,&nbsp;Ying Zhan ,&nbsp;Ren Yang ,&nbsp;Jiao Ren ,&nbsp;Wen Wang ,&nbsp;Baoying Huang ,&nbsp;Wenjie Tan","doi":"10.1016/j.bsheal.2023.06.003","DOIUrl":"https://doi.org/10.1016/j.bsheal.2023.06.003","url":null,"abstract":"<div><p>West Nile virus (WNV) is a mosquito-transmitted flavivirus distributed globally for decades and can cause disease in humans and animals. So far, no WNV vaccine has been licensed for human use. Therefore, the development of novel candidate vaccines and the improvement of vaccination strategies is imperative. As the WNV envelope (E) glycoprotein plays an important role in mediating viral binding to cellular receptors and virus-cell membrane fusion, it is a critical target for the host humoral response. Here, we prepared a recombinant truncated envelope protein of WNV (rWNV-80E) and developed a WNV subunit vaccine formulation with a combination of aluminum hydroxide (alum) and a synthetic oligonucleotide CpG as adjuvants. C57BL/6 mice were immunized twice intramuscularly at 28-day intervals with 5 µg purified rWNV-80E adjuvanted with Alum/CpG. WNV E-specific IgG was detected by enzyme-linked immunosorbent assay and neutralizing antibodies (nAbs) was detected using single-round infectious particles of WNV. Furthermore, T cell immunity was detected by enzyme-linked immunospot assay and intracellular cytokine staining assay. Notably, rWNV-80E was highly immunogenic and elicited potent humoral and cell immunity, as evidenced by significant levels of IFN-γ and TNF-α secretion in the T cells of mice. In summary, the Alum/CpG-adjuvanted rWNV-80E subunit vaccine elicited potent and balanced B- and T-cell immunity in mice, and therefore it is a promising candidate vaccine that warrants further investigation for use in human or veterinary applications.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 300-307"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67739761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of innate immunity in SARS-CoV-2 infection","authors":"Zihao Wang ,&nbsp;Fang Cheng ,&nbsp;Yuxiu Xu ,&nbsp;Xin Li ,&nbsp;Songdong Meng","doi":"10.1016/j.bsheal.2023.08.005","DOIUrl":"https://doi.org/10.1016/j.bsheal.2023.08.005","url":null,"abstract":"<div><p>During severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, activated macrophages, dendritic cells (D.C.), neutrophils, and natural killer (N.K.) cells are the first defense against infection. These immune effectors trap and ingest the virus, kill infected epithelial cells, or produce anti-viral cytokines. Evidence suggests that aging, obesity, and mental illness can lead to weakened innate immunity and, thus, are all associated with elevated infection and severe disease progression of coronavirus disease 2019 (COVID-19). Innate immune defense networks play a fundamental role in suppressing viral replication, infection establishment, and viral pathogenesis of SARS-CoV-2 and other respiratory viruses.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 280-288"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67739770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the roles of TLR7, a nucleic acid sensor for COVID-19 in pan-cancer","authors":"Zhijian Huang ,&nbsp;Yaoxin Gao ,&nbsp;Yuanyuan Han ,&nbsp;Jingwen Yang ,&nbsp;Can Yang ,&nbsp;Shixiong Li ,&nbsp;Decong Zhou ,&nbsp;Qiuyan Huang ,&nbsp;Jialiang Yang","doi":"10.1016/j.bsheal.2023.05.004","DOIUrl":"10.1016/j.bsheal.2023.05.004","url":null,"abstract":"<div><p>Recent studies suggested that cancer was a risk factor for coronavirus disease 2019 (COVID-19). Toll-like receptor 7 (TLR7), a severe acute respiratory syndrome 2 (SARS-CoV-2) virus's nucleic acid sensor, was discovered to be aberrantly expressed in many types of cancers. However, its expression pattern across cancers and association with COVID-19 has not been systematically studied. In this study, we proposed a computational framework to comprehensively study the roles of TLR7 in COVID-19 and pan-cancers at genetic, gene expression, protein, epigenetic, and single-cell levels. We found TLR7 mRNA expression was significantly up-regulated in 6 cancer types and down-regulated in 6 cancer types, further validated in the HPA database at the protein level. The genes significantly co-expressed with TLR7 were mainly enriched in the toll-like receptor signaling pathway, endolysosome, and signaling pattern recognition receptor activity. In addition, the abnormal TLR7 expression was associated with Mismatch repair (MMR), microsatellite instability (MSI), tumor mutational burden (TMB) in various cancers. Mined by the ESTIMATE algorithm, the expression of TLR7 was also closely linked to various immune infiltration patterns in pan-cancer, and TLR7 was mainly enriched in macrophages, as revealed by single-cell RNA sequencing. Finally, TLR7 expressions were very sensitive to a few targeted drugs, such as Alectinib and Imiquimod. In conclusion, TLR7 might be essential in the pathogenesis of COVID-19 and cancers.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 211-226"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9695706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early warning of emerging infectious diseases based on multimodal data","authors":"Haotian Ren ,&nbsp;Yunchao Ling ,&nbsp;Ruifang Cao ,&nbsp;Zhen Wang ,&nbsp;Yixue Li ,&nbsp;Tao Huang","doi":"10.1016/j.bsheal.2023.05.006","DOIUrl":"10.1016/j.bsheal.2023.05.006","url":null,"abstract":"<div><p>The coronavirus disease 2019 (COVID-19) pandemic has dramatically increased the awareness of emerging infectious diseases. The advancement of multiomics analysis technology has resulted in the development of several databases containing virus information. Several scientists have integrated existing data on viruses to construct phylogenetic trees and predict virus mutation and transmission in different ways, providing prospective technical support for epidemic prevention and control. This review summarized the databases of known emerging infectious viruses and techniques focusing on virus variant forecasting and early warning. It focuses on the multi-dimensional information integration and database construction of emerging infectious viruses, virus mutation spectrum construction and variant forecast model, analysis of the affinity between mutation antigen and the receptor, propagation model of virus dynamic evolution, and monitoring and early warning for variants. As people have suffered from COVID-19 and repeated flu outbreaks, we focused on the research results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses. This review comprehensively viewed the latest virus research and provided a reference for future virus prevention and control research.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 193-203"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9687588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection of enterovirus-D68 and vaccine-related poliovirus 3 in the stool samples of a 5-month hospitalized child with acute respiratory disease: A case report","authors":"Wenzhe Su ,&nbsp;Qing Zeng ,&nbsp;Jinmei Geng ,&nbsp;Jingwen Liu ,&nbsp;Huaping Xie ,&nbsp;Kuibiao Li ,&nbsp;Pengzhe Qin ,&nbsp;Chaojun Xie ,&nbsp;Biao Di","doi":"10.1016/j.bsheal.2023.07.002","DOIUrl":"10.1016/j.bsheal.2023.07.002","url":null,"abstract":"<div><p>Human enterovirus (EV) infections can lead to various manifestations, with variable correlations between genotypes and symptoms. Human enterovirus D68 (EV-D68) was considered to be associated with acute respiratory disease and acute flaccid myelitis. In this short report, both EV-D68 and poliovirus 3 were detected in the stool of a hospitalized 5-month child who presented with acute respiratory symptoms and who was recently vaccinated with oral polio vaccine (OPV), using a metatranscriptomic high-throughput sequencing method. The nearly full-length genome sequences with complete open reading frames of EV-D68 and poliovirus 3 were assembled. One previously-reported neurovirulence-related amino acid substitution (T860N) in the EV-D68 VP1 region was observed, but the patient showed no neurological symptoms. More attention should be paid to EV-D68, and continuous multiple syndrome-based surveillance on non-polio enterovirus is called for.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 250-253"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41381250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}