Vaccinia virus viability under different environmental conditions and different disinfectants treatment

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Biosafety and Health Pub Date : 2024-02-01 DOI:10.1016/j.bsheal.2023.12.005

Shan Mei , Liang Wei , Yu Xie , Fei Zhao , Yu Huang , Zhangling Fan , Yamei Hu , Liming Wang , Lingwa Wang , Ying Wang , Fengwen Xu , Fei Guo

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引用次数: 0

Abstract

Monkeypox (mpox) outbreak in 2022 has caused more than 91,000 cases, has spread to 115 countries, regions, and territories, and has thus attracted much attention. The stability of poxvirus particles in the environment is recognized as an important factor in determining their transmission. However, few studies have investigated the persistence of poxviruses on material surfaces under various environmental conditions, and their sensitivity to biocides. Here, we systematically measured the stability of vaccinia virus (VACV) under different environmental conditions and sensitivity to inactivation methods via plaque assay, quantitative real-time polymerase chain reaction (qPCR), and Gaussia luciferase (G-luciferase) reporter system. The results show that VACV is stable on the surface of stainless steel, glass, clothing, plastic, towel, A4 paper, and tissue and persists much longer at 4 °C and −20 °C, but is effectively inactivated by ultraviolet (UV) irradiation, heat treatment, and chemical reagents. Our study raises the awareness of long persistence of poxviruses in the environment and provides a simple solution to inactivate poxviruses using common disinfectants, which is expected to help the control and prevention of mpox virus and future poxvirus outbreaks.

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不同环境条件和不同消毒剂处理下的疫苗病毒活力

2022 年爆发的猴痘疫情已造成 91,000 多例病例，波及 115 个国家、地区和领土，因而备受关注。痘病毒颗粒在环境中的稳定性被认为是决定其传播的重要因素。然而，很少有研究调查痘病毒在各种环境条件下在材料表面的持久性及其对杀菌剂的敏感性。在此，我们通过斑块检测法、定量实时聚合酶链反应（qPCR）和高斯荧光素酶（G-luciferase）报告系统，系统地测定了疫苗病毒（VACV）在不同环境条件下的稳定性以及对灭活方法的敏感性。结果表明，VACV 在不锈钢、玻璃、衣服、塑料、毛巾、A4 纸和纸巾表面都很稳定，在 4 ℃ 和 -20 ℃ 下的存活时间更长，但紫外线（UV）照射、热处理和化学试剂都能有效灭活。我们的研究提高了人们对痘病毒在环境中长期存活的认识，并提供了一种使用普通消毒剂灭活痘病毒的简单解决方案，有望帮助控制和预防痘病毒和未来痘病毒的爆发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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