Cells and Development最新文献_第7页

Developmental roles of natriuretic peptides and their receptors 钠尿肽及其受体的发育作用。

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-22 DOI: 10.1016/j.cdev.2023.203878

Hugo Juraver-Geslin , Arun Devotta , Jean-Pierre Saint-Jeannet

引用次数: 0

Corrigendum to “Single-cell RNA sequencing of mouse lower respiratory tract epithelial cells: A meta-analysis” [Cells Dev. 174C (2023) 203847] “小鼠下呼吸道上皮细胞的单细胞RNA测序:meta分析”的勘误表[cells Dev. 174C (2023) 203847]

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-10 DOI: 10.1016/j.cdev.2023.203877

Leila R. Martins , Hanno Glimm , Claudia Scholl

引用次数: 0

Human pluripotent stem cell-based models of heart development and disease 基于人类多能干细胞的心脏发育和疾病模型

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203857

Gabriel Velichkova , Gergana Dobreva

引用次数: 0

Inflammation and oxidative stress impair preimplantation embryonic morphogenesis in allergic asthma model 炎症和氧化应激对过敏性哮喘模型着床前胚胎形态发生的影响

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203864

Che Ismail Wafriy , Yuhaniza Shafinie Kamsani , Mohamed Noor Khan Nor-Ashikin

{"title":"Inflammation and oxidative stress impair preimplantation embryonic morphogenesis in allergic asthma model","authors":"Che Ismail Wafriy , Yuhaniza Shafinie Kamsani , Mohamed Noor Khan Nor-Ashikin","doi":"10.1016/j.cdev.2023.203864","DOIUrl":"10.1016/j.cdev.2023.203864","url":null,"abstract":"<div>The incidence of allergic asthma has been increasing worldwide in recent decades. Also, an increasing number of women are suffering from poor pregnancy outcome. However, the causal relationship between allergic asthma and embryonic growth in terms of cell morphogenesis has not been well elucidated. Here, we investigated the impact of allergic asthma on the morphogenesis of preimplantation embryos. Twenty-four female BALB/c were randomly divided into control (PBS), 50-μg (OVA1), 100-μg (OVA2) and 150-μg (OVA3). On Days-0 and -14, mice were induced intraperitoneally (i.p) with ovalbumin (OVA). On Days-21 until −23, mice were challenged with OVA via intranasal instillation (i.n). Control animals were sensitized and challenged with PBS. At the end of treatment (Day-25), 2-cell embryos were retrieved and cultured in vitro until the blastocysts hatched. Results showed reduced number of preimplantation embryos at all developing stages in all treated groups (p ≤ 0.0001). Uneven blastomere size, partial compaction- and cavitation-activity, low formation of trophectoderm (TE), as well as cell fragmentation were noted in all the treated groups. Maternal serum interleukin (IL)-4, immunoglobulin (Ig)-E and 8-hydroxydeoxyguanosine (8-OHdG) were notably high (p ≤ 0.0001, p ≤ 0.01) in contrast with low total antioxidant capacity (TAOC) (p ≤ 0.0001). Our findings indicated that OVA-induced allergic asthma had compromised cell morphogenesis through reduced blastomere cleavage division, partial compaction and cavitation-activity, impairment of TE production, and cell fragmentation leading to embryonic cell death via OS mechanism.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203864"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9854274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation tracing identifies hematopoietic regeneration from multipotent progenitors but not stem cells 分化追踪发现造血再生来自多能祖细胞，而不是干细胞

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203861

Tamar Nizharadze , Katrin Busch , Ann-Kathrin Fanti , Hans-Reimer Rodewald , Thomas Höfer

{"title":"Differentiation tracing identifies hematopoietic regeneration from multipotent progenitors but not stem cells","authors":"Tamar Nizharadze , Katrin Busch , Ann-Kathrin Fanti , Hans-Reimer Rodewald , Thomas Höfer","doi":"10.1016/j.cdev.2023.203861","DOIUrl":"10.1016/j.cdev.2023.203861","url":null,"abstract":"<div>Hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) generate the immune system in development, and contribute to its maintenance under steady-state conditions. How stem and progenitor cells respond to increased demand for mature cells upon injury is a fundamental question of stem cell biology. Several studies of murine hematopoiesis have reported increased proliferation of HSCs in situ when exposed to inflammatory stimuli, which has been taken as a proxy for increased HSC differentiation. Such surplus generation of HSC may fuel enhanced HSC differentiation or, alternatively, maintain HSC cellularity in the face of increased cell death without enhanced HSC differentiation. This key question calls for direct measurements of HSC differentiation in their natural niches in vivo. Here, we review work that quantifies native HSC differentiation by fate mapping and mathematical inference. Recent differentiation tracing studies show that HSC do not increase their differentiation rate upon a wide range of challenges, including systemic bacterial infection (sepsis), blood loss, and transient or persistent ablation of specific mature immune cells. By contrast, MPPs differentiate more rapidly in response to systemic infection to accelerate the production of myeloid cells. These new in vivo data identify MPPs as a major source of hematopoietic regeneration; HSCs might not contribute to regeneration while remaining protected.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203861"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10172648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The molecular evolution of mammalian spermatogenesis 哺乳动物精子发生的分子进化

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203865

Nils Trost , Noe Mbengue , Henrik Kaessmann

引用次数: 0

Intestinal stem cells and their niches in homeostasis and disease 肠道干细胞及其生态位在体内平衡和疾病中的作用

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203862

Jun Zhou , Michael Boutros

{"title":"Intestinal stem cells and their niches in homeostasis and disease","authors":"Jun Zhou , Michael Boutros","doi":"10.1016/j.cdev.2023.203862","DOIUrl":"10.1016/j.cdev.2023.203862","url":null,"abstract":"<div>Tissues such as the intestine harbor stem cells that have remarkable functional plasticity in response to a dynamic environment. To adapt to the environment, stem cells constantly receive information from their surrounding microenvironment (also called the ‘niche’) that instructs them how to adapt to changes. The Drosophila midgut shows morphological and functional similarities to the mammalian small intestine and has been a useful model system to study signaling events in stem cells and tissue homeostasis. In this review, we summarize the current understanding of the Drosophila midgut regarding how stem cells communicate with microenvironmental niches including enteroblasts, enterocytes, enteroendocrine cells and visceral muscles to coordinate tissue regeneration and homeostasis. In addition, distant cells such as hemocytes or tracheal cells have been shown to interact with stem cells and influence the development of intestinal diseases. We discuss the contribution of stem cell niches in driving or counteracting disease progression, and review conceptual advances derived from the Drosophila intestine as a model for stem cell biology.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203862"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9795319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Bub3 and Nup75 in the Drosophila male germline lineage 果蝇雄性种系中Bub3和Nup75的分析

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203863

Kerem Yildirim , Patrick van Nierop y Sanchez , Ingrid Lohmann

{"title":"Analysis of Bub3 and Nup75 in the Drosophila male germline lineage","authors":"Kerem Yildirim , Patrick van Nierop y Sanchez , Ingrid Lohmann","doi":"10.1016/j.cdev.2023.203863","DOIUrl":"10.1016/j.cdev.2023.203863","url":null,"abstract":"<div>Extensive communication at the stem cell-niche interface and asymmetric stem cell division is key for the homeostasis of the Drosophila male germline stem cell system. To improve our understanding of these processes, we analysed the function of the mitotic checkpoint complex (MCC) component Bub3 and the nucleoporin Nup75, a component of the nuclear pore complex realizing the transport of signalling effector molecules to the nucleus, in the Drosophila testis. By lineage-specific interference, we found that the two genes control germline development and maintenance. Bub3 is continuously required in the germline, as its loss results in the beginning in an over-proliferation of early germ cells and later on in loss of the germline. The absence of the germline lineage in such testes has dramatic cell non-autonomous consequences, as cells co-expressing markers of hub and somatic cyst cell fates accumulate and populate in extreme cases the whole testis. Our analysis of Nups showed that some of them are critical for lineage maintenance, as their depletion results in the loss of the affected lineage. In contrast, Nup75 plays a role in controlling proliferation of early germ cells but not differentiating spermatogonia and seems to be involved in keeping hub cells quiescent. In sum, our analysis shows that Bub3 and Nup75 are required for male germline development and maintenance.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203863"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10172652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D imaging reveals apical stem cell responses to ambient temperature 三维成像显示顶端干细胞对环境温度的反应

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203850

Christian Wenzl, Jan U. Lohmann

{"title":"3D imaging reveals apical stem cell responses to ambient temperature","authors":"Christian Wenzl, Jan U. Lohmann","doi":"10.1016/j.cdev.2023.203850","DOIUrl":"10.1016/j.cdev.2023.203850","url":null,"abstract":"<div>Plant growth is driven by apical meristems at the shoot and root growth points, which comprise continuously active stem cell populations. While many of the key factors involved in homeostasis of the shoot apical meristem (SAM) have been extensively studied under artificial constant growth conditions, only little is known how variations in the environment affect the underlying regulatory network. To shed light on the responses of the SAM to ambient temperature, we combined 3D live imaging of fluorescent reporter lines that allowed us to monitor the activity of two key regulators of stem cell homeostasis in the SAM namely CLAVATA3 (CLV3) and WUSCHEL (WUS), with computational image analysis to derive morphological and cellular parameters of the SAM. Whereas CLV3 expression marks the stem cell population, WUS promoter activity is confined to the organizing center (OC), the niche cells adjacent to the stem cells, hence allowing us to record on the two central cell populations of the SAM. Applying an integrated computational analysis of our data we found that variations in ambient temperature not only led to specific changes in spatial expression patterns of key regulators of SAM homeostasis, but also correlated with modifications in overall cellular organization and shoot meristem morphology.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203850"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell starvation regulates ceramide-induced autophagy in mouse preimplantation embryo development 细胞饥饿调节神经酰胺诱导的小鼠着床前胚胎自噬

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-09-01 DOI: 10.1016/j.cdev.2023.203859

Seung-Eun Lee , Eun-Seo Lim , Jae-Wook Yoon , Hyo-Jin Park , So-Hee Kim , Han-Bi Lee , Dong-Hun Han , Eun-Young Kim , Se-Pill Park

{"title":"Cell starvation regulates ceramide-induced autophagy in mouse preimplantation embryo development","authors":"Seung-Eun Lee , Eun-Seo Lim , Jae-Wook Yoon , Hyo-Jin Park , So-Hee Kim , Han-Bi Lee , Dong-Hun Han , Eun-Young Kim , Se-Pill Park","doi":"10.1016/j.cdev.2023.203859","DOIUrl":"10.1016/j.cdev.2023.203859","url":null,"abstract":"<div>Ceramide induces autophagy upon starvation via downregulation of nutrient transporters. To elucidate the mechanism by which starvation regulates autophagy in mouse embryos, the present study investigated nutrient transporter expression and the effect of C2-ceramide on in vitro embryo development, apoptosis, and autophagy. The transcript levels of the glucose transporters Glut1 and Glut3 were high at the 1- and 2-cell stages, and gradually decreased at the morula and blastocyst (BL) stages. Similarly, expression of the amino acid transporters L-type amino transporter-1 (LAT-1) and 4F2 heavy chain (4F2hc) gradually decreased from the zygote to the BL stage. Upon ceramide treatment, expression of Glut1, Glut3, LAT-1, and 4F2hc was significantly reduced at the BL stage, while expression of the autophagy-related genes Atg5, LC3, and Gabarap and synthesis of LC3 were significantly induced. Ceramide-treated embryos exhibited significantly reduced developmental rates and total cell numbers per blastocyst, and increased levels of apoptosis and expression of Bcl2l1 and Casp3 at the BL stage. Ceramide treatment significantly decreased the average mitochondrial DNA copy number and mitochondrial area at the BL stage. In addition, ceramide treatment significantly decreased mTOR expression. These results suggest that ceramide-induced autophagy promotes apoptosis by following downregulation of nutrient transporters during mouse embryogenesis.</div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203859"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9786509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0