Cells and Development最新文献

Morphogenetic constraints in the development of gastruloids: Implications for mouse gastrulation 类原肠发育的形态发育限制：对小鼠原肠形成的影响

IF 2 4区生物学

Cells and Development Pub Date : 2025-09-01 DOI: 10.1016/j.cdev.2025.204043

Ulla-Maj Fiuza , Sara Bonavia , Pau Pascual-Mas , Gabriel Torregrosa-Cortés , Pablo Casaní-Galdón , Gaëlle Robertson , André Dias , Alfonso Martinez Arias

{"title":"Morphogenetic constraints in the development of gastruloids: Implications for mouse gastrulation","authors":"Ulla-Maj Fiuza , Sara Bonavia , Pau Pascual-Mas , Gabriel Torregrosa-Cortés , Pablo Casaní-Galdón , Gaëlle Robertson , André Dias , Alfonso Martinez Arias","doi":"10.1016/j.cdev.2025.204043","DOIUrl":"10.1016/j.cdev.2025.204043","url":null,"abstract":"<div><div>Mammalian embryonic size is tightly controlled with checkpoints and compensatory mechanisms correcting size defects. Here, we take advantage of gastruloids, a stem cell embryoid system not subject to most size controls, to study the role of size in emergent properties of mammalian embryogenesis. We report that gastruloids exhibit robust morphology and transcriptional profiles within a size range. However, size affects the dynamics, and, outside a range of robust morphogenesis, the precision of anterior-posterior (AP) axial elongation. Gastruloid axial elongation exhibits active cellular contractility, requires planar cell polarity (PCP), adhesion and cell-cell contact remodelling. Smaller gastruloids initiate elongation earlier, correlated with an earlier Brachyury polarisation. Brachyury expression increases tissue fluidity. Axis formation is regulated by the balance of Brachyury multifoci coalescence and the timing of initiation of the elongation programme. Sizes beyond the robust range can modify relative tissue composition. Very small aggregates have increased neural fate bias, accompanied by a loss of paraxial mesoderm mediated by differences in Nodal signalling activity.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"183 ","pages":"Article 204043"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelial differentiation of Oral cavity-derived mesenchymal stem/stromal cells 口腔源性间充质干细胞/间质细胞的内皮分化。

IF 2 4区生物学

Cells and Development Pub Date : 2025-09-01 DOI: 10.1016/j.cdev.2025.204041

Wen Jun Thang

{"title":"Endothelial differentiation of Oral cavity-derived mesenchymal stem/stromal cells","authors":"Wen Jun Thang","doi":"10.1016/j.cdev.2025.204041","DOIUrl":"10.1016/j.cdev.2025.204041","url":null,"abstract":"<div><div>A rapid construction of vasculature ensures the survival and proper functions of tissue-engineered constructs for oral and craniofacial tissue regeneration. Vascularization achieved via a co-culture system containing endothelial cells (ECs) and other supporting cells is more advantageous compared to host angiogenic ingrowth, as it allows faster vessel formation or even prevascularization. Among different sources of ECs, the multidifferentiation capacity of oral cavity-derived mesenchymal stem/stromal cells (MSCs) indicates their potential to give rise to autologous ECs for use in vascular tissue engineering. Considering the growing interest in vascularization in tissue engineering, this review intends to provide a comprehensive overview of the endothelial differentiation of human oral cavity-derived MSCs. Specifically, the author begins by scrutinizing the scientific justification and biological feasibility of employing MSC-derived ECs, followed by an exploration of the criteria and assessment of both in vitro and in vivo endothelial differentiation and a compilation of different strategies for endothelial differentiation induction and their underlying mechanisms. Subsequently, the different aspects pertinent to the practical considerations, including the characteristics and optimization of endothelial differentiation protocols/strategies, culture conditions, and cell origin and subpopulations, are also discussed to guide the selection of the differentiation approach and to understand the factors affecting the differentiation outcome. Finally, the present technical, biological, and clinical constraints and future perspectives are also provided. Gaining a comprehensive knowledge of these topics may provide a cornerstone for the potential derivation of functional and mature ECs from oral cavity-derived MSCs, which will certainly provide clinical value for oral and craniofacial tissue regeneration.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"183 ","pages":"Article 204041"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information 封底外-图形摘要TOC/双栏TOC/封面图例（如适用），条形码，摘要和索引信息

IF 2 4区生物学

Cells and Development Pub Date : 2025-09-01 DOI: 10.1016/S2667-2901(25)00057-9

引用次数: 0

The characterization of the biological effect of hypoxia-mimetic condition on angiogenic potential in mesenchymal stem cells derived from different origins 模拟缺氧条件对不同来源间充质干细胞血管生成潜能的生物学影响。

IF 2 4区生物学

Cells and Development Pub Date : 2025-09-01 DOI: 10.1016/j.cdev.2025.204039

Yeo Gyun Yun , Donghyeon Yeo , Trang Thanh Thien Tran , Seong-Jin Shin , Ji-Sun Shin , Nam Kyu Lim , Jun Hee Lee , Hae-Won Kim

{"title":"The characterization of the biological effect of hypoxia-mimetic condition on angiogenic potential in mesenchymal stem cells derived from different origins","authors":"Yeo Gyun Yun , Donghyeon Yeo , Trang Thanh Thien Tran , Seong-Jin Shin , Ji-Sun Shin , Nam Kyu Lim , Jun Hee Lee , Hae-Won Kim","doi":"10.1016/j.cdev.2025.204039","DOIUrl":"10.1016/j.cdev.2025.204039","url":null,"abstract":"<div><div>Under physiological conditions, adult stem cells are located in a special microenvironment, known as the niche, which is characterized by low oxygen levels. Hypoxia is a crucial factor regulating stem cell behavior, and in vitro normoxic culture often leads to the loss of pluripotency, resulting in reduced therapeutic potential of cells. In this study, we aimed to investigate the effects of cobalt chloride (CoCl<sub>2</sub>), a hypoxia-mimetic agent, on mesenchymal stem cells (MSCs) derived from different sources by inducing hypoxia-inducible factor-1α. Cell proliferation, migration, survival, and metabolic activity were assessed. CoCl<sub>2</sub> treatment decreased the proliferation and migration and enhanced the survival of MSCs. Furthermore, a metabolic shift from oxidative phosphorylation to glycolysis was observed in cells. Overall, our findings provide important insights into the mechanisms by which hypoxic conditions differentially influence stem cells, facilitating the improvement of their therapeutic potential.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"183 ","pages":"Article 204039"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental programming by maternal obesity alters offspring lifespan and immune responses in a diet- and sex-specific manner 由母体肥胖引起的发育程序以饮食和性别特异性的方式改变后代的寿命和免疫反应。

IF 3.9 4区生物学

Cells and Development Pub Date : 2025-07-11 DOI: 10.1016/j.cdev.2025.204040

Seyhmus Bayar , Lea Seep , Karolína Doubková , Jelena Zurkovic , Margret H. Bülow , Katrin Kierdorf , Reinhard Bauer , Christoph Thiele , Gaia Tavosanis , Jan Hasenauer , Elvira Mass

{"title":"Developmental programming by maternal obesity alters offspring lifespan and immune responses in a diet- and sex-specific manner","authors":"Seyhmus Bayar , Lea Seep , Karolína Doubková , Jelena Zurkovic , Margret H. Bülow , Katrin Kierdorf , Reinhard Bauer , Christoph Thiele , Gaia Tavosanis , Jan Hasenauer , Elvira Mass","doi":"10.1016/j.cdev.2025.204040","DOIUrl":"10.1016/j.cdev.2025.204040","url":null,"abstract":"<div><div>Maternal obesity is a growing health concern that predisposes offspring to metabolic dysfunction, immune system alterations, and neurodegenerative disorders. To investigate the intergenerational effects of maternal obesity, we used <em>Drosophila melanogaster</em> exposed to high-sugar (HSD) and high-fat diets (HFD) before mating. We found that maternal diet-induced obesity significantly altered offspring lifespan, immune responses, and neuronal health in a sex- and diet-specific manner. Male offspring were particularly susceptible, exhibiting reduced lifespan, impaired climbing ability, and increased axonal degeneration, especially following maternal HFD exposure. Transcriptomic analyses revealed age-dependent and diet-specific changes, with males showing pronounced alterations at 50 days of age. Developmental programming of hemocytes (blood-like cells) played a crucial role in these outcomes, as knockdown of key immune pathways such as <em>Relish</em> and <em>upd3</em> in hemocytes further influenced lifespan in a diet-specific manner. These findings highlight the complex interplay between maternal diet and immune function, underscoring the impact of maternal obesity-induced imprinting on immune cells and subsequent long-term health consequences. Our study provides new insights into conserved mechanisms linking maternal metabolic health to offspring outcomes and emphasizes the continued need for animal models to understand intergenerational health impacts.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"183 ","pages":"Article 204040"},"PeriodicalIF":3.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sall1 regulates microtubule acetylation in mesenchymal cells during mouse urethral development 在小鼠尿道发育过程中，Sall1调控间充质细胞的微管乙酰化。

IF 3.9 4区生物学

Cells and Development Pub Date : 2025-06-01 DOI: 10.1016/j.cdev.2025.204027

Alvin R. Acebedo , Gen Yamada , Mellissa C. Alcantara , Dennis D. Raga , Tetsuya Sato , Ryuichi Nishinakamura , Kentaro Suzuki

{"title":"Sall1 regulates microtubule acetylation in mesenchymal cells during mouse urethral development","authors":"Alvin R. Acebedo , Gen Yamada , Mellissa C. Alcantara , Dennis D. Raga , Tetsuya Sato , Ryuichi Nishinakamura , Kentaro Suzuki","doi":"10.1016/j.cdev.2025.204027","DOIUrl":"10.1016/j.cdev.2025.204027","url":null,"abstract":"<div><div>Male embryonic external genitalia (eExG) undergo sexually dimorphic urethral development in response to androgen signaling (urethral masculinization). Whereas androgen is an essential masculinization factor for eExG, the specific molecular and cellular mechanisms are still unclear. <em>Sall1</em> is a transcription factor that has been linked to the congenital disease Townes-Brocks syndrome, which includes anorectal and urogenital malformations. Currently, the functional role of <em>Sall1</em> for normal urethral development is still unclear. In this study, we show that <em>Sall1</em> is required to regulate proper microtubule acetylation to facilitate mesenchymal cell migration during urethral masculinization of mouse eExG. Mutant male mice with loss of function of mesenchymal <em>Sall1</em> exhibited severe urethral defects, without prominent alteration of androgen signaling. Loss of <em>Sall1</em> induced hyperacetylated microtubules in the eExG mesenchyme. Microtubule hyperacetylation resulted in defective fibrillar adhesions and fibronectin expression which impaired cell migration. Our findings reveal a novel mechanism of Sall1-regulated mesenchymal cell migration for urethral development. This mechanism for Sall1 may underlie the etiology of diseases such as Townes-Brocks syndrome.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"182 ","pages":"Article 204027"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Definition of the characteristic neurogenesis pattern in the neural plate by the Hes1 orthologue gene, her6, during early zebrafish development 在斑马鱼早期发育过程中，Hes1同源基因her6在神经板中的特征性神经发生模式的定义。

IF 3.9 4区生物学

Cells and Development Pub Date : 2025-06-01 DOI: 10.1016/j.cdev.2025.204026

Momo Tsuruoka, Hiroki Tokizaki, Kyo Yamasu

{"title":"Definition of the characteristic neurogenesis pattern in the neural plate by the Hes1 orthologue gene, her6, during early zebrafish development","authors":"Momo Tsuruoka, Hiroki Tokizaki, Kyo Yamasu","doi":"10.1016/j.cdev.2025.204026","DOIUrl":"10.1016/j.cdev.2025.204026","url":null,"abstract":"<div><div>During vertebrate embryonic development, a distinctive, spotty neurogenesis pattern emerges in the early neural plate, which represents proneural clusters. The determination of this pattern depends on the interaction between proneural genes and bHLH-O-type transcription factor (TF) genes, <em>Hes/her</em>, which suppress neurogenesis. In this study, we focused on the mouse <em>Hes1</em> orthologue, <em>her6</em>, to understand the mechanism that controls neurodevelopmental patterns in the developing brain in zebrafish (<em>Danio rerio</em>). We first assessed the expression pattern of <em>her6</em> in the neural plate, observing that it is consistently expressed in the entire forebrain throughout somitogenesis, including <em>her9</em> expression within it. Meanwhile, the expression patterns of <em>her6</em> changed dynamically in the hindbrain, in contrast to the Notch-independent <em>her</em> genes. The expression pattern was not significantly affected by forced NICD expression and DAPT treatment at the bud stage, showing that <em>her6</em> expression is Notch-independent in the neural plate at this stage. To analyze the roles of <em>her6</em>, we disrupted <em>her6</em> using the CRISPR/Cas9 method. The mutants thus obtained showed a deformed midbrain-hindbrain region and failed to grow to adulthood. At the bud stage, ectopic expression of neurogenesis-related genes was observed in <em>her6</em> mutants in specific regions of the neural plate, where neurogenesis does not occur and which are considered neural progenitor pools (NPPs) in wild-type embryos. Of note, no other Notch-independent <em>her</em> genes are known to be expressed in these NPP regions. In contrast, the expression of regionalization genes in the forebrain and hindbrain was not affected in <em>her6</em> mutants. These findings suggest that <em>her6</em> defines the primary neurogenesis pattern in the neural plate, together with other Notch-independent <em>her</em> genes.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"182 ","pages":"Article 204026"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The novel role of foxi3 in zebrafish mandibular development foxi3 在斑马鱼下颌骨发育过程中的新作用

IF 3.9 4区生物学

Cells and Development Pub Date : 2025-06-01 DOI: 10.1016/j.cdev.2025.204016

Xin Chen , Run Yang , Tianyu Zhang , Jing Ma

引用次数: 0

Generation of kidney organoids derived from human expanded potential stem cells 从人类扩增潜能干细胞中生成肾脏器官组织。

IF 3.9 4区生物学

Cells and Development Pub Date : 2025-06-01 DOI: 10.1016/j.cdev.2025.204025

Zhanpeng Kuang , Changmiao Pang , Haiyan Wang , Xiaocui Wei , Xianhua Ye , Xuefei Gao , Liangzhong Sun

引用次数: 0

Extracellular matrix in cardiac morphogenesis, fibrosis, and regeneration 细胞外基质在心脏形态发生、纤维化和再生中的作用。