Cells and Development最新文献_第2页

Physiological effect of cytokines on adult neurogenesis: a focus on in vivo knock-out studies 细胞因子对成人神经发生的生理作用：体内敲除研究的焦点。

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI: 10.1016/j.cdev.2026.204072

Narjess Haidar , Laure Salvon, Amnah Al-Sayyar, Oussama Kassem, Audrey Romano, Rejane Rua

{"title":"Physiological effect of cytokines on adult neurogenesis: a focus on in vivo knock-out studies","authors":"Narjess Haidar , Laure Salvon, Amnah Al-Sayyar, Oussama Kassem, Audrey Romano, Rejane Rua","doi":"10.1016/j.cdev.2026.204072","DOIUrl":"10.1016/j.cdev.2026.204072","url":null,"abstract":"<div><div>Cytokines, central mediators of immune signaling, have emerged as pivotal regulators of neural stem cell (NSC) biology. Once regarded as immune-privileged, the central nervous system is now recognized as a dynamic site of immune-neural interaction, where meningeal and parenchymal immune cells modulate neurogenesis and brain plasticity through cytokine release. This review synthesizes current quantitative evidence on the effects of key cytokines including IL-6, FGF2, IL-15, IL-22, IL-10, and TNF-α on mouse NSCs studied exclusively in knockout models <em>in vivo</em>. This approach allows for a clearer understanding of their physiological roles, minimizing artefacts associated with <em>in vitro</em> systems, overexpression models, or inflammatory contexts, and focusing instead on their homeostatic functions. Collectively, these studies demonstrate that cytokine signaling profoundly influences NSC fate, proliferation, and differentiation. Importantly, by mapping cytokine expression within the dentate gyrus, the subventricular zone neurogenic niches, and the meninges, we put forward the provocative idea that brain borders are major sources of these factors, rather than the dentate gyrus itself. Understanding how immune-derived cues shape the NSC niche is critical for uncovering mechanisms underlying brain function, plasticity, and repair, with potential implications for neurodevelopmental, neuropsychiatric, and neurodegenerative disorders.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"185 ","pages":"Article 204072"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles: A complex array of particles involved in cell-to-cell communication for tissue homeostasis 细胞外囊泡：一种复杂的粒子阵列，参与组织内稳态的细胞间通讯。

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2025-10-24 DOI: 10.1016/j.cdev.2025.204054

Natayme Rocha Tartaglia , Lorena Martin-Jaular , Alain Joliot , Clotilde Théry

{"title":"Extracellular vesicles: A complex array of particles involved in cell-to-cell communication for tissue homeostasis","authors":"Natayme Rocha Tartaglia , Lorena Martin-Jaular , Alain Joliot , Clotilde Théry","doi":"10.1016/j.cdev.2025.204054","DOIUrl":"10.1016/j.cdev.2025.204054","url":null,"abstract":"<div><div>Communication between cells is an inescapable feature of every multicellular organism, from the coordination of development during embryogenesis to the maintenance of homeostasis throughout adulthood. Extracellular vesicles (EVs) are new and central players in intercellular communication processes, by carrying multiple signals at once and acting in concert with well-described soluble effectors released in the environment. These membrane-enclosed particles are released by all cells in their environment. Their lipid bilayer protects their internal content while exposing surface determinants, allowing EVs to interact with target cells and/or surrounding extracellular matrix. EVs can also escape to blood or lymph circulation and reach further away organs. By carrying multiple signals, locally or at a distance, EVs are increasingly considered as players in the coordination of physiological processes within and across tissues. Here, we briefly summarize the many studies describing physiological functions of EVs, and discuss their actual demonstration <em>in vivo</em>. In addition, we discuss this knowledge in light of our current understanding on the heterogeneity of EVs, the complex composition of EV-containing preparations and the consequences to unravel the specific activities of EVs, including subtypes of EVs such as exosomes, and of other extracellular particles (EPs).</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"185 ","pages":"Article 204054"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145459633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LSM14A, an LSM family protein, is dispensable for spermatogenesis and male fertility in mice LSM14A是LSM家族的一种蛋白，在小鼠的精子发生和雄性生育中是必不可少的。

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2025-11-12 DOI: 10.1016/j.cdev.2025.204058

Xu Fan, Ling Yang, Xiao Wang, Nana Li, Zhengpin Wang

{"title":"LSM14A, an LSM family protein, is dispensable for spermatogenesis and male fertility in mice","authors":"Xu Fan, Ling Yang, Xiao Wang, Nana Li, Zhengpin Wang","doi":"10.1016/j.cdev.2025.204058","DOIUrl":"10.1016/j.cdev.2025.204058","url":null,"abstract":"<div><div>The LSM (Like-Smith) family comprises RNA-binding proteins (RBPs) that are key regulators of RNA metabolism. LSM14A, a member of this family (designated <em>Lsm14a</em> in mice), participates in RNA processing within cytoplasmic processing bodies (P-bodies). The mouse <em>Lsm14a</em> gene is localized to chromosome 7qB1, spans 48.67 kilobases (kb), and encodes a 462-amino-acid protein that exhibits 94.53 % amino acid identity with human LSM14A. However, the expression profile of LSM14A in male reproductive organs and its functional relevance to male fertility remain uncharacterized. In this study, we report that LSM14A is expressed in the mouse testis and localizes to the cytoplasm of germ cells, from spermatogonia to elongating spermatids. To investigate LSM14A function, we generated germ cell-specific <em>Lsm14a</em> conditional knockout (cKO) mice. <em>Lsm14a</em> cKO male mice displayed normal growth, development, and fertility. Histological examination of <em>Lsm14a</em> cKO testes revealed preserved spermatogenesis and seminiferous tubule structure. <em>Lsm14a</em> cKO sperm exhibited normal morphology, acrosome integrity, and motility. The loss of <em>Lsm14a</em> in the testes did not significantly affect P-body formation, suggesting that genetic compensation by other LSM family members may have been activated upon <em>Lsm14a</em> knockout, thereby compensating for its loss of function. Collectively, these findings demonstrate that LSM14A is dispensable for spermatogenesis and male fertility in mice.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"185 ","pages":"Article 204058"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145524318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information 封底外-图形摘要TOC/双栏TOC/封面图例（如适用），条形码，摘要和索引信息

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2026-03-09 DOI: 10.1016/S2667-2901(26)00010-0

引用次数: 0

From initiation to maturation: Mesenchymal stem cells as key facilitators in organoid development 从起始到成熟：间充质干细胞在类器官发育中的关键促进作用

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.cdev.2026.204071

Shihao Yi , Juan Wen , Tianshun Wang , Wan Yu , Yanlin Liao , Zhengyun Liu , Huan Wang

引用次数: 0

Mechanical conditions preventing live cell extrusion during primary neurulation in amniotes 羊膜原代神经发育过程中防止活细胞挤压的机械条件。

IF 2 4区生物学

Cells and Development Pub Date : 2026-03-01 Epub Date: 2025-11-27 DOI: 10.1016/j.cdev.2025.204059

Santiago A. Bosch-Roascio , Julio A. Hernández , Flavio R. Zolessi

{"title":"Mechanical conditions preventing live cell extrusion during primary neurulation in amniotes","authors":"Santiago A. Bosch-Roascio , Julio A. Hernández , Flavio R. Zolessi","doi":"10.1016/j.cdev.2025.204059","DOIUrl":"10.1016/j.cdev.2025.204059","url":null,"abstract":"<div><div>During primary neurulation in amniote embryos, the neural plate gives rise to the neural tube in a process requiring the coordination of forces at different scales throughout a geometrically complex tissue. The ways in which this process fails inform us of the complex mechanical conditions required for its correct completion. Previous results showed that the functional disruption of MARCKS, a protein which simultaneously interacts with the plasma membrane and actin filaments, resulted in neural tube closure defects with apical cell extrusion. Here, we demonstrate that this is an example of “live cell extrusion”, wherein extruded cells are not undergoing apoptosis. This suggests that extrusion in this case might be due to a mechanical instability in the neural plate. Using an expanded energy-based vertex model of pseudostratified epithelia we then show that extrusion may be elicited by a reduction in the relative surface tension of apical and basal interfaces with respect to cell-cell interfaces. Finally, by considering a continuum description of a simplified epithelium we derive an approximate quantitative threshold for single-layered epithelial stability in the form of a power law relating cell density to the relative value of interfacial surface tensions. Our work serves to explain an example of how alterations in polarization and forces at the single-cell level can produce tissue-scale instabilities which not only greatly alter its morphology but can also ultimately lead to severe developmental defects.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"185 ","pages":"Article 204059"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell biology of the chick organizer: Origins, composition, population dynamics and fate 小鸡组织者的细胞生物学：起源，组成，种群动态和命运。

IF 2 4区生物学

Cells and Development Pub Date : 2025-12-01 Epub Date: 2025-03-03 DOI: 10.1016/j.cdev.2025.204017

Claudio D. Stern

{"title":"Cell biology of the chick organizer: Origins, composition, population dynamics and fate","authors":"Claudio D. Stern","doi":"10.1016/j.cdev.2025.204017","DOIUrl":"10.1016/j.cdev.2025.204017","url":null,"abstract":"<div><div>The year 2024 celebrates 100 years of perhaps one of the most important and influential papers in the field of developmental biology: Spemann and Mangold's publication reporting the discovery of the “organizer”, which can induce and pattern the nervous system and also pattern the axial-lateral axis of the mesoderm. While many papers have investigated, and many others reviewed, the signalling aspects of the organizer, relatively fewer have concentrated on the cell biology of organizer cells. Here we survey more than 12 decades of knowledge on the chick organizer, including the cellular origins, fates, composition, cell movements, cell population properties and molecular dynamics of the chick organizer (the tip of the primitive streak). What emerges is a picture of an extremely complex and dynamic population of cells whose properties change over space and time, quite different from the “textbook” view of a static group of cells set aside during early development to perform a particular function in the normal embryo before being swept aside. Some of these findings also have more general implications for the interpretation of results from single cell RNA sequencing experiments.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 204017"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal control of embryonic dorsal organizer in vertebrates 脊椎动物胚胎背器官的母性控制。

IF 2 4区生物学

Cells and Development Pub Date : 2025-12-01 Epub Date: 2025-03-07 DOI: 10.1016/j.cdev.2025.204020

Jing Chen , Anming Meng

{"title":"Maternal control of embryonic dorsal organizer in vertebrates","authors":"Jing Chen , Anming Meng","doi":"10.1016/j.cdev.2025.204020","DOIUrl":"10.1016/j.cdev.2025.204020","url":null,"abstract":"<div><div>The establishment of the body axis and developmental blueprint in embryos has remained to be a central question in developmental biology, captivating scientists for centuries. A milestone in this field was achieved in 1924 when Hans Spemann and Hilde Mangold discovered the dorsal organizer for embryonic body axis formation in amphibians. Since then, extensive studies have demonstrated that the dorsal organizer is evolutionarily conserved in vertebrates. This organizer functions as a signaling center, directing adjacent cells toward specific fates and orchestrating pattern formation to establish the embryonic axis. After 70 years since the discovery of the organizer, studies in different model animal species had revealed that locally activated β-catenin signaling during blastulation plays an indispensable role in organizer induction. Then, efforts have been made to identify initiators of β-catenin activation in blastulas. Now, it appears that maternal Huluwa, a transmembrane protein, is a bona fide organizer inducer at least in teleost fish and frog, which can activate downstream signaling pathways, including but probably not limited to β-catenin pathway. More studies are needed to decode the complete molecular network controlling organizer induction.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 204020"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-organization from organs to embryoids by activin in early amphibian development 早期两栖动物发育过程中器官到胚状体的自组织。

IF 2 4区生物学

Cells and Development Pub Date : 2025-12-01 Epub Date: 2025-01-23 DOI: 10.1016/j.cdev.2025.203996

Makoto Asashima , Yumeko Satou-Kobayashi , Yoshikazu Haramoto , Takashi Ariizumi

{"title":"Self-organization from organs to embryoids by activin in early amphibian development","authors":"Makoto Asashima , Yumeko Satou-Kobayashi , Yoshikazu Haramoto , Takashi Ariizumi","doi":"10.1016/j.cdev.2025.203996","DOIUrl":"10.1016/j.cdev.2025.203996","url":null,"abstract":"<div><div>Embryonic development is a complex self-organizing process orchestrated by a series of regulatory events at the molecular and cellular levels, resulting in the formation of a fully functional organism. This review focuses on activin protein as a mesoderm-inducing factor and the self-organizing properties it confers. Activin has been detected in both unfertilized eggs and embryos, suggesting its involvement in early developmental processes. To explore its effects, animal cap cells—pluripotent cells from the animal pole of amphibian blastula-stage embryos—were treated with varying concentrations of activin. The results showed that activin induced mesodermal tissues, including blood, muscle, and notochord, in a dose-dependent manner. Co-treatment with activin and retinoic acid further promoted the development of kidney and pancreatic tissues, while activin alone stimulated the formation of beating cardiac tissue. In subsequent experiments, high concentrations of activin conferred an organizer-like activity on animal cap cells. The pretreatment duration affected outcomes: longer exposure induced anterior structures, such as eyes, while shorter exposure resulted in posterior structures, like tails. These findings reflect moderate self-assembly, where cells become increasingly organized. In another experiment, activin was used to create an artificial gradient. Explants cultured on this gradient developed into embryoids with well-defined anteroposterior, dorsoventral, and left-right axes, exemplifying higher-order self-organization. These results demonstrate that controlled activin gradients can drive the formation of nearly complete tadpole-like larvae, effectively recapitulating the processes of early embryogenesis. This system offers valuable insights into the mechanisms underlying axis formation and organogenesis, providing a promising platform for future research in developmental biology.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 203996"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt and BMP signaling pathways pattern the anterior neuroectoderm of the indirect-developing hemichordate Ptychodera flava Wnt和BMP信号通路模式的前神经外胚层间接发展的半硬掌黄掌。

IF 2 4区生物学

Cells and Development Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1016/j.cdev.2025.204060

Yann Le Petillon , Tzu-Pei Fan , Yi-Chih Chen , Yi-Hsien Su

{"title":"Wnt and BMP signaling pathways pattern the anterior neuroectoderm of the indirect-developing hemichordate Ptychodera flava","authors":"Yann Le Petillon , Tzu-Pei Fan , Yi-Chih Chen , Yi-Hsien Su","doi":"10.1016/j.cdev.2025.204060","DOIUrl":"10.1016/j.cdev.2025.204060","url":null,"abstract":"<div><div>The chordate gastrula organizer establishes Wnt and BMP signaling gradients that are essential for generating the chordate body plan. In non-chordate deuterostomes, including echinoderms and hemichordates, these two signals also contribute to body axial patterning, although it remains debatable whether there is a <em>bona fide</em> equivalent tissue to the organizer. One consequence of these patterning mechanisms is the formation of a neuron population originating from the anterior neuroectoderm (ANE). Wnt signaling is essential for restricting the ANEs in sea urchins with planktonic larval stages as well as direct-developing hemichordates (without larval stages). However, it is difficult to infer the ancestral mechanisms in deuterostomes since information is lacking regarding the mechanisms of ANE formation in indirect-developing hemichordates (with larval stages). Here, we delineate the deployment of Wnt signaling components as well as the roles of Wnt and BMP signals in ANE development in the indirect-developing hemichordate <em>Ptychodera flava</em>. We show that posterior Wnt signaling functions to pattern the anteroposterior axis and restrict the ANE during gastrulation. Meanwhile, BMP signaling promotes ANE development and regeneration during gastrulation, although it initially represses the formation of neural tissues. Our findings thus support a conserved role for Wnt signaling in ANE restriction and suggest a biphasic function of BMP signaling during ANE formation, providing insights into the patterning mechanisms within the common ancestor of deuterostomes.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 204060"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145574791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0