Cells and Development最新文献

{"title":"Peroxiredoxin 5 overexpression decreases oxidative stress and dopaminergic cell death mediated by paraquat","authors":"Ana Patricia Duarte-Jurado,&nbsp;Maria de Jesus Loera-Arias,&nbsp;Odila Saucedo-Cardenas,&nbsp;Roberto Montes de Oca-Luna,&nbsp;Humberto Rodriguez-Rocha,&nbsp;Aracely Garcia-Garcia","doi":"10.1016/j.cdev.2023.203860","DOIUrl":"10.1016/j.cdev.2023.203860","url":null,"abstract":"<div><p><span><span><span>Peroxiredoxins<span> (Prdxs) are thiol-dependent enzymes that scavenge peroxides. Previously, we found that Prdxs were hyperoxidized in a </span></span>Parkinson's disease model induced by paraquat (PQ), which led to their inactivation, perpetuating </span>reactive oxygen species<span><span> (ROS) formation. Herein, we evaluated the redox state of the typical 2-Cys-Prx subgroup. We found that PQ induces ROS compartmentalization in different organelles, reflected by the 2-Cys-Prdx hyperoxidation pattern detected by redox eastern blotting. 2-Cys Prdxs are most vulnerable to hyperoxidation, while atypical 2-Cys Peroxiredoxin 5 (Prdx5) is resistant and is expressed in multiple organelles, such as mitochondria, </span>peroxisomes<span>, and cytoplasm. Therefore, we overexpressed human Prdx5 in the dopaminergic SHSY-5Y cell line using the </span></span></span>adenoviral vector<span><span> Ad-hPrdx5. Prdx5 overexpression was confirmed by western blotting and immunofluorescence (IF) and effectively decreased PQ-mediated mitochondrial and cytoplasmic ROS assessed with a mitochondrial superoxide indicator and DHE through IF or flow cytometry. Decreased ROS mediated by Prdx5 in the main subcellular compartments led to overall cell protection against PQ-induced cell death, which was demonstrated by flow cytometry using </span>Annexin V<span> labeling and 7-AAD. Therefore, Prdx5 is an attractive therapeutic target for PD, as its overexpression protects dopaminergic cells from ROS and death, which warrants further experimental animal studies for its subsequent application in clinical trials.</span></span></p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203860"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9786498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disrupted neurogenesis, gliogenesis, and ependymogenesis in the Ccdc85c knockout rat for hydrocephalus model","authors":"Md. Mehedi Hasan,&nbsp;Shizuka Konishi,&nbsp;Miyuu Tanaka,&nbsp;Takeshi Izawa,&nbsp;Jyoji Yamate,&nbsp;Mitsuru Kuwamura","doi":"10.1016/j.cdev.2023.203858","DOIUrl":"10.1016/j.cdev.2023.203858","url":null,"abstract":"<div><p>Coil-coiled domain containing 85c (<em>Ccdc85c</em><span>) is a causative gene for congenital hydrocephalus and subcortical heterotopia with frequent brain hemorrhage. We established </span><em>Ccdc85c</em><span><span><span> knockout (KO) rats and investigated the roles of CCDC85C and intermediate filament<span> protein expression, including </span></span>nestin<span>, vimentin, GFAP, and </span></span>cytokeratin<span> AE1/AE3 during the lateral ventricle<span> development in KO rats to evaluate the role of this gene. We found altered and ectopic expression<span><span> of nestin and vimentin positive cells in the wall of the dorso-lateral ventricle in the KO rats during development from the age of postnatal day (P) 6, whereas both protein expression became faint in the wild-type rats. In the KO rats, there was a loss of cytokeratin expression on the surface of the dorso-lateral ventricle with ectopic expression and maldevelopment of ependymal cells<span>. Our data also revealed disturbed GFAP expression at postnatal ages. These findings indicate that lack of CCDC85C disrupts the proper expression of intermediate filament proteins (nestin, vimentin, GFAP, and cytokeratin), and CCDC85C is necessary for normal neurogenesis, </span></span>gliogenesis, and ependymogenesis.</span></span></span></span></p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"175 ","pages":"Article 203858"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9795323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LIN28 is essential for the maintenance of chicken primordial germ cells","authors":"Katsuya Suzuki ,&nbsp;Seung June Kwon ,&nbsp;Daisuke Saito,&nbsp;Yuji Atsuta","doi":"10.1016/j.cdev.2023.203874","DOIUrl":"10.1016/j.cdev.2023.203874","url":null,"abstract":"<div><p>Understanding the mechanism of stem cell maintenance underlies the establishment of long-term and mass culture methods for stem cells that are fundamental for clinical and agricultural applications. In this study, we use chicken primordial germ cell (PGC) as a model to elucidate the molecular mechanisms underlying stem cell maintenance. The PGC is a useful experimental model because it is readily gene-manipulatable and easy to test gene function <em>in vivo</em><span> using transplantation. Previous studies to establish a long-term culture system have shown that secreted factors such as FGF2<span> are required to maintain the self-renewal capability of PGC. On the other hand, we know little about intracellular regulators responsible for PGC maintenance. Among representative stem cell factors<span>, we focus on RNA-binding factors LIN28A and LIN28B as possible central regulators for the gene regulatory network<span> essential to PGC maintenance. By taking advantage of the CRISPR/Cas9-mediated gene editing and a clonal culture technique, we find that both LIN28A and LIN28B regulate the proliferation of PGC </span></span></span></span><em>in vitro</em>. We further showed that colonization efficiency of grafted PGC at the genital ridges, rudiments for the gonads, of chicken embryos were significantly decreased by knockout (KO) of <em>LIN28A</em> or <em>LIN28B</em><span>. Of note, overexpression of human LIN28<span> in LIN28-KO PGC was sufficient to restore the low colonization rates, suggesting that LIN28 plays a key role in PGC colonization at the gonads. Transcriptomic analyses of LIN28-KO PGC reveal that several genes related to mesenchymal traits are upregulated, including </span></span><span><em>EGR1</em></span>, a transcription factor that promotes the differentiation of mesodermal tissues. Finally, we show that the forced expression of human EGR1 deteriorates replication activity and colonization efficiency of PGCs. Taken together, this work demonstrates that LIN28 maintains self-renewal of PGC by suppressing the expression of differentiation genes including <em>EGR1</em>.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"176 ","pages":"Article 203874"},"PeriodicalIF":3.9,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9834076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Enhanced autophagy suppresses inflammation-mediated bone loss through ROCK1 signaling in bone marrow mesenchymal stem cells” [Cells Dev. 167 (2021) 203687]","authors":"Jingjing Zheng ,&nbsp;Yuli Gao ,&nbsp;Haozhi Lin ,&nbsp;Changqing Yuan ,&nbsp;Keqian Zhi","doi":"10.1016/j.cdev.2023.203867","DOIUrl":"10.1016/j.cdev.2023.203867","url":null,"abstract":"","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"176 ","pages":"Article 203867"},"PeriodicalIF":3.9,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constructing the pharyngula: Connecting the primary axial tissues of the head with the posterior axial tissues of the tail","authors":"Geneva Masak ,&nbsp;Lance A. Davidson","doi":"10.1016/j.cdev.2023.203866","DOIUrl":"10.1016/j.cdev.2023.203866","url":null,"abstract":"<div><p><span>The pharyngula stage of vertebrate development is characterized by stereotypical arrangement of ectoderm, </span>mesoderm<span>, and neural tissues from the anterior spinal cord to the posterior, yet unformed tail. While early embryologists over-emphasized the similarity between vertebrate embryos at the pharyngula stage, there is clearly a common architecture upon which subsequent developmental programs generate diverse cranial structures and epithelial appendages such as fins, limbs, gills, and tails. The pharyngula stage is preceded by two morphogenetic events: gastrulation<span> and neurulation, which establish common shared structures despite the occurrence of cellular processes that are distinct to each of the species. Even along the body axis of a singular organism, structures with seemingly uniform phenotypic characteristics at the pharyngula stage have been established by different processes. We focus our review on the processes underlying integration of posterior axial tissue formation with the primary axial tissues that creates the structures laid out in the pharyngula. Single cell sequencing and novel gene targeting technologies have provided us with new insights into the differences between the processes that form the anterior and posterior axis, but it is still unclear how these processes are integrated to create a seamless body. We suggest that the primary and posterior axial tissues in vertebrates form through distinct mechanisms and that the transition between these mechanisms occur at different locations along the anterior-posterior axis. Filling gaps that remain in our understanding of this transition could resolve ongoing problems in organoid culture and regeneration.</span></span></p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"176 ","pages":"Article 203866"},"PeriodicalIF":3.9,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9856722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in understanding the impact of infection and inflammation on hematopoietic stem and progenitor cells","authors":"Michael D. Milsom ,&nbsp;Marieke A.G. Essers","doi":"10.1016/j.cdev.2023.203844","DOIUrl":"10.1016/j.cdev.2023.203844","url":null,"abstract":"<div><p>Just over one decade ago, it was discovered that hematopoietic stem cells (HSCs) could directly respond to inflammatory cytokines by mounting a proliferative response thought to mediate the emergency production of mature blood cells. In the intervening years, we have gained mechanistic insight into this so-called activation process and have started to learn such a response may come at a cost in terms of ultimately resulting in HSC exhaustion and hematologic dysfunction. In this review article, we report the progress we have made in understanding the interplay between infection, inflammation and HSCs during the funding period of the Collaborative Research Center 873 “Maintenance and Differentiation of Stem Cells in Development and Disease”, and place this work within the context of recent output by others working within this field.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"174 ","pages":"Article 203844"},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and clonal characterization of neural stem cell niche recruitment in the medaka neuromast","authors":"Jasmin Onistschenko,&nbsp;Sabrina Kaminsky,&nbsp;Javier Vazquez-Marín,&nbsp;Karen Gross,&nbsp;Tianyu Wang ,&nbsp;Ali Seleit ,&nbsp;Melanie Dörr ,&nbsp;Lázaro Centanin","doi":"10.1016/j.cdev.2023.203837","DOIUrl":"10.1016/j.cdev.2023.203837","url":null,"abstract":"<div><p>Stem cell populations are defined by their capacity to self-renew and to generate differentiated progeny. These unique characteristics largely depend on the stem cell micro-environment, the so-called stem cell niche. Niches were identified for most adult stem cells studied so far, but we know surprisingly little about how somatic stem cells and their niche come together during organ formation. Using the neuromasts of teleost fish, we have previously reported that neural stem cells recruit their niche from neighboring epithelial cells, which go through a morphological and molecular transformation. Here, we tackle quantitative, temporal, and clonal aspects of niche formation in neuromasts by using 4D imaging in transgenic lines, and lineage analysis in mosaic fish. We show that niche recruitment happens in a defined temporal window during the formation of neuromasts in medaka, and after that, the niche is enlarged mainly by the proliferation of niche cells. Niche recruitment is a non-clonal process that feeds from diverse epithelial cells that do not display a preferential position along the circumference of the forming neuromast. Additionally, we cover niche formation and expansion in zebrafish to show that distant species show common features during organogenesis in the lateral line system. Overall, our findings shed light on the process of niche formation, fundamental for the maintenance of stem cells not only in medaka but also in many other multicellular organisms.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"174 ","pages":"Article 203837"},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9536708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep machine learning for cell segmentation and quantitative analysis of radial plant growth","authors":"Alexandra Zakieva ,&nbsp;Lorenzo Cerrone ,&nbsp;Thomas Greb","doi":"10.1016/j.cdev.2023.203842","DOIUrl":"10.1016/j.cdev.2023.203842","url":null,"abstract":"<div><p>Plants produce the major part of terrestrial biomass and are long-term deposits of atmospheric carbon. This capacity is to a large extent due to radial growth of woody species – a process driven by cambium stem cells located in distinct niches of shoot and root axes. In the model species <em>Arabidopsis thaliana</em>, thousands of cells are produced by the cambium in radial orientation generating a complex organ anatomy enabling long-distance transport, mechanical support and protection against biotic and abiotic stressors. These complex organ dynamics make a comprehensive and unbiased analysis of radial growth challenging and asks for tools for automated quantification. Here, we combined the recently developed PlantSeg and MorphographX image analysis tools, to characterize tissue morphogenesis of the <em>Arabidopsis</em> hypocotyl. After sequential training of segmentation models on ovules, shoot apical meristems and adult hypocotyls using deep machine learning, followed by the training of cell type classification models, our pipeline segments complex images of transverse hypocotyl sections with high accuracy and classifies central hypocotyl cell types. By applying our pipeline on both wild type and <em>phloem intercalated with xylem</em> (<em>pxy</em>) mutants, we also show that this strategy faithfully detects major anatomical aberrations. Collectively, we conclude that our established pipeline is a powerful phenotyping tool comprehensively extracting cellular parameters and providing access to tissue topology during radial plant growth.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"174 ","pages":"Article 203842"},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9544442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical biomarkers for human hematopoietic stem and progenitor cells","authors":"Motomu Tanaka ,&nbsp;Judith Thoma ,&nbsp;Laura Poisa-Beiro ,&nbsp;Patrick Wuchter ,&nbsp;Volker Eckstein ,&nbsp;Sascha Dietrich ,&nbsp;Caroline Pabst ,&nbsp;Carsten Müller-Tidow ,&nbsp;Takao Ohta ,&nbsp;Anthony D. Ho","doi":"10.1016/j.cdev.2023.203845","DOIUrl":"10.1016/j.cdev.2023.203845","url":null,"abstract":"<div><p>Adhesion of hematopoietic stem and progenitor cells (HSPCs) to the bone marrow niche plays critical roles in the maintenance of the most primitive HSPCs. The interactions of HSPC−niche interactions are clinically relevant in acute myeloid leukemia (AML), because (i) leukemia-initiating cells adhered to the marrow niche are protected from the cytotoxic effect by chemotherapy and (ii) mobilization of HSPCs from healthy donors' bone marrow is crucial for the effective stem cell transplantation. However, although many clinical agents have been developed for the HSPC mobilization, the effects caused by the extrinsic molecular cues were traditionally evaluated based on phenomenological observations. This review highlights the recent interdisciplinary challenges of hematologists, biophysicists and cell biologists towards the design of defined <em>in vitro</em> niche models and the development of physical biomarkers for quantitative indexing of differential effects of clinical agents on human HSPCs.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"174 ","pages":"Article 203845"},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9544138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematics of neural stem cells: Linking data and processes","authors":"Diana-Patricia Danciu ,&nbsp;Jooa Hooli ,&nbsp;Ana Martin-Villalba ,&nbsp;Anna Marciniak-Czochra","doi":"10.1016/j.cdev.2023.203849","DOIUrl":"10.1016/j.cdev.2023.203849","url":null,"abstract":"<div><p>Adult stem cells are described as a discrete population of cells that stand at the top of a hierarchy of progressively differentiating cells. Through their unique ability to self-renew and differentiate, they regulate the number of end-differentiated cells that contribute to tissue physiology. The question of how discrete, continuous, or reversible the transitions through these hierarchies are and the precise parameters that determine the ultimate performance of stem cells in adulthood are the subject of intense research. In this review, we explain how mathematical modelling has improved the mechanistic understanding of stem cell dynamics in the adult brain. We also discuss how single-cell sequencing has influenced the understanding of cell states or cell types. Finally, we discuss how the combination of single-cell sequencing technologies and mathematical modelling provides a unique opportunity to answer some burning questions in the field of stem cell biology.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"174 ","pages":"Article 203849"},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9898932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}