Cells and Development最新文献_第9页

Divergent tooth development mechanisms of Mexican tetra fish (Astyanax mexicanus) of Pachón cave origin Pachón穴居起源的墨西哥四目鱼(Astyanax mexicanus)牙齿分化发育机制

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2022.203823

Devi Atukorallaya, Vikram Bhatia, Jessica Gonzales

{"title":"Divergent tooth development mechanisms of Mexican tetra fish (Astyanax mexicanus) of Pachón cave origin","authors":"Devi Atukorallaya, Vikram Bhatia, Jessica Gonzales","doi":"10.1016/j.cdev.2022.203823","DOIUrl":"10.1016/j.cdev.2022.203823","url":null,"abstract":"<div><p>The Mexican tetra (<em>Astyanax mexicanus</em>) is one of the fresh water teleost fish models in evolutionary developmental biology. The existence of two morphs: eyed, pigmented surface fish and blind depigmented cavefish from multiple cave populations, provides a unique system to study adaptive radiation. Compared to the adult surface fish, cavefish have large oral jaws with an increased number of structurally-complex teeth. Early tooth development has not been studied in detail in cavefish populations. In this study, bone-stained growth series and vital dye staining was used to trace the development and replacement of dentitions in Pachón cavefish. Our results show that first tooth eruption was delayed in cavefish compared to the surface fish. In particular, the first tooth eruption cycle persisted until 35 days post fertilization (dpf). Unlike surface fish, there are multicuspid teeth in cavefish first generation dentition. In addition to the teeth in the marginal oral jaw bones, Pachón cavefish have teeth in the ectopterygoid bone of the palatine roof. Next, we characterised the expression of ectodysplasin signalling pathway genes in tooth-forming regions of surface and cavefish. Interestingly, higher expression of Eda and Edar was found in cavefish compared to the surface fish. The altered ectodysplasin expression needs further investigation to confirm the different molecular mechanisms for tooth development in the oral and pharyngeal regions of surface fish and cavefish.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9152393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPD2: The relationship with cancer and neural stemness GPD2:与肿瘤和神经干性的关系

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2022.203824

Maimaiti Mikeli , Makoto Fujikawa , Tsutomu Tanabe

引用次数: 0

5-Azacytidine incorporated skeletal muscle-derived hydrogel promotes rat skeletal muscle regeneration 5-氮胞苷结合骨骼肌衍生水凝胶促进大鼠骨骼肌再生

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2023.203826

Behnaz Mirza Ahmadi , Afshin Noori , Mohammad Kazemi Ashtiani , Sarah Rajabi , Mahmood Talkhabi

{"title":"5-Azacytidine incorporated skeletal muscle-derived hydrogel promotes rat skeletal muscle regeneration","authors":"Behnaz Mirza Ahmadi , Afshin Noori , Mohammad Kazemi Ashtiani , Sarah Rajabi , Mahmood Talkhabi","doi":"10.1016/j.cdev.2023.203826","DOIUrl":"10.1016/j.cdev.2023.203826","url":null,"abstract":"<div><p>Decellularized skeletal muscle is a promising biomaterial for muscle regeneration due to the mimicking of the natural microenvironment. Previously, it has been reported that 5-Azacytidine (5-Aza), a DNA methyltransferase inhibitor, induces myogenesis in different types of stem cells. In the current study, we investigated the effect of 5-Aza incorporated muscle-derived hydrogel on the viability and proliferation of muscle-derived stem cells (MDSCs) <em>in vitro</em> and muscle regeneration <em>in vivo</em>. Wistar rat skeletal muscles were decellularized using a physico-chemical protocol. The decellularized tissue was analyzed using SEM, histological staining and evaluation of DNA content. Then, muscle-derived hydrogel was made from Pepsin-digested decellularized muscle tissues. 5-Aza was physically adsorbed in prepared hydrogels. Then, MDSCs were cultured on hydrogels with/without 5-Aza, and their proliferation and cell viability were determined using LIVE/DEAD and DAPI staining. Moreover, myectomy lesions were done in rat femoris muscles, muscle-derived hydroges with/without 5-Aza were injected to the myectomy sites, and histological evaluation was performed after three weeks. The analysis of decellularized muscle tissues showed that they maintained extracellular matrix components of native muscles, while they lacked DNA. LIVE/DEAD and DAPI staining showed that the hydrogel containing 5-Aza supported MDSCs viability. Histological analysis of myectomy sites showed an improvement in muscle regeneration after administration of 5-Aza incorporated hydrogel. These findings suggest that the combination of 5-Aza with skeletal muscle hydrogel may serve as an alternative treatment option to improve the regeneration of injured muscle tissue.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9208355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pvr regulates cyst stem cell division in the Drosophila testis niche, and has functions distinct from Egfr Pvr在果蝇睾丸壁龛中调控囊肿干细胞分裂，具有不同于Egfr的功能

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2022.203822

Nastaran Mues, Kenneth Hammer, Judith Leatherman

{"title":"Pvr regulates cyst stem cell division in the Drosophila testis niche, and has functions distinct from Egfr","authors":"Nastaran Mues, Kenneth Hammer, Judith Leatherman","doi":"10.1016/j.cdev.2022.203822","DOIUrl":"10.1016/j.cdev.2022.203822","url":null,"abstract":"<div><p>Regulation of the rate of stem cell division is one of the key determinants of the abundance of differentiating progeny in stem cell-supported tissues, and mis-regulation can lead to tumorigenesis. The well-studied <em>Drosophila</em> testis niche is an excellent model system to study the regulation of stem cell division in vivo. This niche supports two stem cell populations—the germline stem cells (GSCs) and cyst stem cells (CySCs), which cluster around a group of cells called the hub. The differentiating cells of these two stem cell populations cooperate together to produce sperm. Signal transduction initiated by the epidermal growth factor receptor (Egfr) is a key regulatory pathway in the cyst lineage, and much of the study of this stem cell population has centered around understanding the complexities of the requirements for Egfr signaling. We examined another receptor tyrosine kinase, Pvr, the sole <em>Drosophila</em> PDGF/VEGF homolog, and found that it accumulates in the cyst lineage cells of the testis, while its ligand Pvf1 accumulates in the hub. Pvr inhibition caused a reduction in both CySC numbers and the proportion of CySCs in S phase, similar to Egfr inhibition. However, testes with Pvr inhibition exhibited a low-penetrance non-autonomous germ cell differentiation defect distinct from that observed with Egfr inhibition. Cyst cells with constitutively activated Pvr failed to support germ cell differentiation, as observed with constitutively activated Egfr. However, constitutively activated Pvr promoted tumorous accumulation of cyst cells outside of the niche, a phenotype not observed with constitutively activated Egfr. Thus, Egfr and Pvr have some receptor-specific functions and some shared functions in the cyst lineage cells of the testis.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9162998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Celebrating Spemann-Mangold at 100: An interview with Eddy De Robertis 庆祝斯潘曼-曼戈尔德100岁:艾迪·德·罗伯蒂斯的采访

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2023.203828

John Williamson

引用次数: 0

Physical models of notochord cell packing reveal how tension ratios determine morphometry 脊索细胞堆积的物理模型揭示了张力比如何决定形态

IF 3.9 4区生物学

Cells and Development Pub Date : 2023-03-01 DOI: 10.1016/j.cdev.2023.203825

Evan J. Curcio, Sharon R. Lubkin

引用次数: 1

Inhibition of TGFβ1/Smad pathway by NF-κB induces inflammation leading to poor wound healing in high glucose NF-κB抑制tgf - β1/Smad通路可诱导炎症，导致高糖患者伤口愈合不良

IF 3.9 4区生物学

Cells and Development Pub Date : 2022-12-01 DOI: 10.1016/j.cdev.2022.203814

Fan Gong , Yun Zhang , Suoli Cheng , Xuebing Zhou , Hanling Zhang , Jian Gao , Xiaoliang Li , Guoxu Ma , Jianke Wu , Bowen Zhang , Kun Xia , Fei Zhao

{"title":"Inhibition of TGFβ1/Smad pathway by NF-κB induces inflammation leading to poor wound healing in high glucose","authors":"Fan Gong , Yun Zhang , Suoli Cheng , Xuebing Zhou , Hanling Zhang , Jian Gao , Xiaoliang Li , Guoxu Ma , Jianke Wu , Bowen Zhang , Kun Xia , Fei Zhao","doi":"10.1016/j.cdev.2022.203814","DOIUrl":"10.1016/j.cdev.2022.203814","url":null,"abstract":"<div><p>This study mainly analyzed the relationship between nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor-β (TGFβ1)/Smad under high glucose environment and its influence on wound healing. Fibroblast NIH-3T3 was used to analyze the effect of high concentration glucose (20 nmol/mL) on cell viability, migration ability, inflammation level and NF-κB pathway. Pyrrolidinedithiocarbamate (PDTC) was used to inhibit NF-κB for rescue experiments. Diabetic mice were used to construct wound healing models. Recombinant TGF-β1 was used to promote wound healing in diabetic mice. FSL-1 was applied to activate NF-κB to verify the mechanism. High glucose inhibited cell viability and migration ability, promoted the expression of TNF-α, IL-6 and IL-1β, induced the activation of NF-κB pathway in fibroblasts. Inhibition of NF-κB not only blocked the decrease in cell viability and migration ability induced by high glucose, but also relieved the release of inflammatory factors. TGF-β1 activated the TGF-β1/Smad pathway and promoted wound healing in diabetic mice. Activating the NF-κB pathway not only inhibited the activation of the TGF-β1/Smad pathway, but also alleviated the promoting effect of TGF-β1 on wound healing. In a high glucose environment, the activation of NF-κB may inhibit the function of fibroblasts by inhibiting the TGF-β1/Smad pathway, resulting in poor wound healing.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usability of deep learning pipelines for 3D nuclei identification with Stardist and Cellpose 使用Stardist和Cellpose进行三维核识别的深度学习管道的可用性

IF 3.9 4区生物学

Cells and Development Pub Date : 2022-12-01 DOI: 10.1016/j.cdev.2022.203806

Giona Kleinberg , Sophia Wang , Ester Comellas , James R. Monaghan , Sandra J. Shefelbine

{"title":"Usability of deep learning pipelines for 3D nuclei identification with Stardist and Cellpose","authors":"Giona Kleinberg , Sophia Wang , Ester Comellas , James R. Monaghan , Sandra J. Shefelbine","doi":"10.1016/j.cdev.2022.203806","DOIUrl":"10.1016/j.cdev.2022.203806","url":null,"abstract":"<div><p><span>Segmentation of 3D images to identify cells and their molecular outputs can be difficult and tedious. Machine learning algorithms provide a promising alternative to manual analysis as emerging 3D image processing technology can save considerable time. For those unfamiliar with machine learning or 3D image analysis, the rapid advancement of the field can make navigating the newest software options confusing. In this paper, two open-source machine learning algorithms, Cellpose and Stardist, are compared in their application on a 3D light sheet dataset counting fluorescently stained </span>proliferative cell nuclei. The effects of image tiling and background subtraction are shown through image analysis pipelines for both algorithms. Based on our analysis, the relative ease of use of Cellpose and the absence of need to train a model leaves it a strong option for 3D cell segmentation despite relatively longer processing times. When Cellpose's pretrained model yields results that are not of sufficient quality, or the analysis of a large dataset is required, Stardist may be more appropriate. Despite the time it takes to train the model, Stardist can create a model specialized to the users' dataset that can be iteratively improved until predictions are satisfactory with far lower processing time relative to other methods.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10570629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

IF 3.9 4区生物学

Cells and Development Pub Date : 2022-12-01 DOI: 10.1016/j.cdev.2022.203805

引用次数: 0

An interview with Fabiola Osorio 对Fabiola Osorio的采访

IF 3.9 4区生物学

Cells and Development Pub Date : 2022-09-01 DOI: 10.1016/j.cdev.2022.203804

John J. Williamson

引用次数: 0