Journal of Functional Foods最新文献_第3页

Sanghuangporus vaninii-fermented green tea targets FoxO1/SIRT1 to alleviate obesity via integrated network pharmacology and multi-omics 三黄茯蓣发酵绿茶通过网络药理学和多组学研究，靶向FoxO1/SIRT1减轻肥胖

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-17 DOI: 10.1016/j.jff.2026.107214

Xuzhou Liu , Ying Ju , Liangliang Qi , Chao Wang , Bingchuan Tian , Mingguo Jiang , Preecha Prathepha , Jianan Huang , Zhonghua Liu

{"title":"Sanghuangporus vaninii-fermented green tea targets FoxO1/SIRT1 to alleviate obesity via integrated network pharmacology and multi-omics","authors":"Xuzhou Liu , Ying Ju , Liangliang Qi , Chao Wang , Bingchuan Tian , Mingguo Jiang , Preecha Prathepha , Jianan Huang , Zhonghua Liu","doi":"10.1016/j.jff.2026.107214","DOIUrl":"10.1016/j.jff.2026.107214","url":null,"abstract":"<div><div>Building on prior evidence of enhanced in vitro lipid-lowering activity of <em>Sanghuangporus vaninii</em>-fermented green tea, this study evaluated its anti-obesity mechanisms in high-fat diet-induced obese mice. Components of the fermented tea were characterized, and network pharmacology predicted potential lipid-modulating targets. Seventy male C57BL/6 J mice were allocated to seven groups: a normal diet group, a high-fat diet group, a high-fat diet group treated with orlistat (positive control), and high-fat diet groups treated with either non-fermented tea or the fermented tea product at two dosages (200 or 400 mg/kg/day). After a four-week intervention, the fermented tea product significantly reduced body weight, serum total cholesterol, and hepatic lipid accumulation compared to the high-fat diet controls. Treatment also enhanced gut microbial diversity, reduced the Firmicutes/Bacteroidetes ratio, and increased fecal triglyceride excretion. Hepatic transcriptomics revealed upregulation of genes including <em>Gadd45a</em> and <em>Bcl6</em>, with pathway analysis confirming involvement in FoxO signaling. Mechanistically, the fermented tea product suppressed hepatic lipogenic genes while upregulating markers of fatty acid oxidation and lipolysis, consistent with network pharmacology predictions. These findings demonstrate that the fermented tea product alleviates diet-induced obesity through coordinated modulation of hepatic lipid metabolism and gut microbiota, outperforming non-fermented tea and providing novel insights into fermented tea as a dietary intervention.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107214"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ergogenic and cardiometabolic benefits of policosanol in male taekwondo athletes: a double-blind trial 对男性跆拳道运动员的有氧和心脏代谢益处：一项双盲试验

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-17 DOI: 10.1016/j.jff.2026.107190

Hamidreza Razmi , Aliakbar Alizadeh , Ahmad Zare Javid , Bijan Helli

{"title":"Ergogenic and cardiometabolic benefits of policosanol in male taekwondo athletes: a double-blind trial","authors":"Hamidreza Razmi , Aliakbar Alizadeh , Ahmad Zare Javid , Bijan Helli","doi":"10.1016/j.jff.2026.107190","DOIUrl":"10.1016/j.jff.2026.107190","url":null,"abstract":"<div><div>High-intensity combat sports such as taekwondo impose considerable oxidative and metabolic stress, potentially impairing recovery and performance. Policosanol has demonstrated antioxidant and metabolic effects in non-athletic populations; however, evidence in trained athletes, particularly regarding performance outcomes, remains limited. In this 12-week randomized, double-blind, placebo-controlled trial, 44 male taekwondo athletes received either policosanol (25 mg/day) or placebo under standardized training conditions. Body composition, lipid profile, oxidative stress markers, aerobic capacity (Cooper test), and anaerobic power (RAST) were assessed before and after intervention. Compared with placebo, policosanol supplementation resulted in a significant reduction in lipid peroxidation (malondialdehyde, MDA). Improvements were also observed in anaerobic performance, VO₂max and Cooper test distance (<em>p</em> < 0.05). Although favorable within-group changes occurred in some antioxidant and lipid parameters, most cardiometabolic variables did not differ significantly between groups after baseline adjustment. Policosanol supplementation may support oxidative stress modulation and selected aspects of athletic performance in trained taekwondo athletes, while cardiometabolic effects appear modest.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107190"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of L-alanine and erythritol synergistically enhances water transport in an in vitro system using human-induced pluripotent stem cell-derived small intestinal epithelial cells l-丙氨酸和赤藓糖醇联合使用人诱导的多能干细胞来源的小肠上皮细胞在体外系统中协同增强水运输

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-11 DOI: 10.1016/j.jff.2026.107202

Tsubasa Mizokami, Shintaro Ono

{"title":"Combination of L-alanine and erythritol synergistically enhances water transport in an in vitro system using human-induced pluripotent stem cell-derived small intestinal epithelial cells","authors":"Tsubasa Mizokami, Shintaro Ono","doi":"10.1016/j.jff.2026.107202","DOIUrl":"10.1016/j.jff.2026.107202","url":null,"abstract":"<div><div>Glucose-containing beverages enhance water absorption from the lumen of the small intestine into capillaries <em>via</em> Na<sup>+</sup>-dependent glucose transporters. However, considering the harmful effects of excessive sugar, water absorption-promoting substances that do not worsen calorie intake and glucose metabolism are desirable. Alternative tools that avoid animal studies and clinical trials are required to quantitatively evaluate such substances. Here, we employed an <em>in vitro</em> system using human induced pluripotent stem (iPS) cell-derived small intestinal epithelial cells (hiPSC-SIECs) to evaluate the effects of glucose, amino acids, erythritol, and allose on water transport. Among the 15 amino acids, L-alanine had the greatest effect. Significant increases in water transport were also observed with erythritol and allose, which are promising sugar substitutes. Furthermore, L-alanine and erythritol showed synergistic effects. Overall, we demonstrate the efficacy of hiPSC-SIECs as an alternative tool for quantitation of water absorption and propose that L-alanine and erythritol can synergistically promote water absorption.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107202"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sea anemone-derived bioactive peptides alleviate ulcerative colitis via remodeling the gut microbiota-metabolite-immune axis 海葵衍生的生物活性肽通过重塑肠道微生物-代谢-免疫轴来缓解溃疡性结肠炎

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-11 DOI: 10.1016/j.jff.2026.107206

Jieqiong Lin , Ting Lu , Dahua Chen , Zhonghua Wang , Jun Zhou , Jiaojiao Han

{"title":"Sea anemone-derived bioactive peptides alleviate ulcerative colitis via remodeling the gut microbiota-metabolite-immune axis","authors":"Jieqiong Lin , Ting Lu , Dahua Chen , Zhonghua Wang , Jun Zhou , Jiaojiao Han","doi":"10.1016/j.jff.2026.107206","DOIUrl":"10.1016/j.jff.2026.107206","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a chronic inflammatory bowel disease with limited therapeutic options. This study investigates the protective effects of bioactive peptides derived from sea anemone (ACPs), prepared by enzymatic hydrolysis, in a dextran sulfate sodium (DSS)-induced murine model of UC. Notably, the peptide ERRK was identified as a dominant component of the ACPs mixture. Remarkably, low-dose ACPs (200 mg/kg) demonstrated superior efficacy over higher doses in ameliorating disease severity, as evidenced by reduced disease activity index (DAI) scores, increased colon length, and improved histopathology. ACPs significantly downregulated pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) and upregulated anti-inflammatory cytokines (TGF-β, IL-10), while restoring intestinal barrier integrity via increased expression of tight junction proteins (ZO-1, Occludin, Claudin-1). Multi-omics analyses revealed that ACPs remodeled gut microbiota composition, enriched beneficial genera (<em>Muribaculum</em>, <em>Prevotella</em>), and modulated host serum metabolites, particularly pathways related to butyrate and tryptophan metabolism. These findings underscore the potential of ACPs, especially at low doses, as a novel functional food for UC management through integrated modulation of the gut microbiota-metabolite-immune axis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107206"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic insights into the antidiabetic effects of mulberry leaf Fu brick tea in GK rats 桑叶茯砖茶对GK大鼠抗糖尿病作用的转录组学研究

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI: 10.1016/j.jff.2026.107212

Fang Zhou , Ji-ming Jia , Ying Liu , Xuan-yu Liu , Xiang-yang Tang , Jie Qin , Peng-yun Fu , Meng-yao Ma , Hua Fu , Bao-de Zhu , Chang-wei Liu

{"title":"Transcriptomic insights into the antidiabetic effects of mulberry leaf Fu brick tea in GK rats","authors":"Fang Zhou , Ji-ming Jia , Ying Liu , Xuan-yu Liu , Xiang-yang Tang , Jie Qin , Peng-yun Fu , Meng-yao Ma , Hua Fu , Bao-de Zhu , Chang-wei Liu","doi":"10.1016/j.jff.2026.107212","DOIUrl":"10.1016/j.jff.2026.107212","url":null,"abstract":"<div><div>Mulberry leaves and Fu brick tea are renowned for their exceptional hypoglycemic effects. This study used mulberry leaves and fu brick tea as raw materials to develop mulberry leaf Fu brick tea (MFT) as a dietary regulator that can assist in the prevention and alleviation of diabetes. The results demonstrate that administration of MFT at a dose of 210 mg/kg/day for a consecutive 4-week period significantly reduces fasting and random blood glucose levels in GK rats. Notably, levels of glycosylated hemoglobin and glycosylated serum protein return to the normal range, while insulin and glucagon levels are significantly increased, accompanied by substantial improvement in glucose tolerance. Moreover, MFT proves effective in regulating dyslipidemia and reducing liver damage. Transcriptomic analysis reveals that MFT enhances insulin sensitivity and promotes glycogen synthesis by up-regulating key genes in the PI3K-Akt and PPAR signaling pathways, including <em>Insr</em>, <em>Ins1</em>, <em>Gys2</em>, <em>Pi3k</em>, <em>Akt</em>, and <em>Pparα</em>. These findings highlight MFT as a potential dietary intervention for glucose regulation, warranting further evaluation in clinical studies.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107212"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactiplantibacillus plantarum Ln4 attenuates streptozotocin and high-fat diet-induced Type-2 diabetes 植物乳杆菌Ln4减轻链脲佐菌素和高脂肪饮食诱导的2型糖尿病

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-18 DOI: 10.1016/j.jff.2026.107219

Rachit Sood , Kritika , Sanjay , Hae-Jeung Lee

{"title":"Lactiplantibacillus plantarum Ln4 attenuates streptozotocin and high-fat diet-induced Type-2 diabetes","authors":"Rachit Sood , Kritika , Sanjay , Hae-Jeung Lee","doi":"10.1016/j.jff.2026.107219","DOIUrl":"10.1016/j.jff.2026.107219","url":null,"abstract":"<div><div>Type-2 diabetes mellitus (T2DM) is an expanding global concern with limited efficacy of conventional therapies. Probiotics have gained attention for their ability to modulate gut microbiota and glucose homeostasis. This study evaluated the therapeutic potential of <em>Lactiplantibacillus plantarum</em> Ln4 at LOW (5 × 10<sup>8</sup> CFU/day) and HIGH (5 × 10<sup>9</sup> CFU/day) doses in an STZ/HFD-induced diabetic mouse model, compared with metformin (200 mg/kg). STZ/HFD induction resulted in reduced body and muscle weight, increased food and water intake, elevated fasting blood glucose, OGTT, AUC, HOMA-IR, liver weight, and dysregulated metabolic biomarkers. Ln4 administration significantly reversed these alterations, normalized serum adiponectin, insulin, GLP-1, and HbA1c levels, and improved lipid profile, oxidative stress markers, and histopathological changes in liver and skeletal muscle. Mechanistically, Ln4 restored GLUT-2, GLUT-4, GCK, IRS/PI3K/Akt, AMPK, and GSK3β signaling while suppressing PEPCK and PTP1B expression. Overall, <em>L. plantarum</em> Ln4 effectively ameliorated hyperglycemia and dyslipidemia, showing efficacy comparable to metformin.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107219"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cirsium setidens attenuates doxorubicin-induced cardiotoxicity by modulating oxidative stress and inflammatory pathways 鸢尾草通过调节氧化应激和炎症途径减轻阿霉素诱导的心脏毒性

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI: 10.1016/j.jff.2026.107197

Ji-Hye Song, Jangho Lee, Min-Sun Kim, Seung-U Son, Hyo-Kyoung Choi

{"title":"Cirsium setidens attenuates doxorubicin-induced cardiotoxicity by modulating oxidative stress and inflammatory pathways","authors":"Ji-Hye Song, Jangho Lee, Min-Sun Kim, Seung-U Son, Hyo-Kyoung Choi","doi":"10.1016/j.jff.2026.107197","DOIUrl":"10.1016/j.jff.2026.107197","url":null,"abstract":"<div><div>Doxorubicin (DOX) is a widely used chemotherapeutic agent, but its clinical application is limited by cardiotoxicity associated with oxidative stress, inflammation, and apoptosis. <em>Cirsium setidens</em> (<em>C. setidens</em>), a traditional Korean edible plant, is known for its antioxidant and anti-inflammatory properties. This study investigated the cardioprotective potential of <em>C. setidens</em> aqueous extract (CSA) against DOX-induced cardiotoxicity (DICT). Metabolite profiling used UPLC-QTOF/MS revealed that CSA contains diverse bioactive compounds, predominantly amino acids and phenolic constituents, including flavonoids, coumarins, cinnamic acid derivatives, and higenamine. Cardiotoxic H9c2 cardiomyocytes and DOX-induced mouse model were used to investigate the in vitro and in vivo effects of CSA, respectively. CSA protected H9c2 cells against DICT while further enhancing DOX's anticancer effect in MDA-MB-231 cells. Moreover, CSA markedly attenuated DOX-induced cardiac dysfunction and myocardial injury, including electrocardiographic abnormalities and myocardial fibrosis. Mechanistically, CSA exerted its cardioprotective effects through coordinated regulation of the ROS-inflammation signaling axis. CSA suppressed inflammatory gene expression, including <em>Il-1β</em>, <em>Il-6</em>, <em>Tnf-α</em>, <em>Ifn-γ</em>, and <em>Mcp-1</em>. Concurrently, it activated the Nrf1/Nrf2 antioxidant signaling pathways, restoring the expression of downstream antioxidant genes such as <em>Sod1</em>, <em>Sod2</em>, and <em>Ho-1</em>. In addition, CSA modulated apoptosis-related genes by downregulating <em>Bax</em>, <em>Casp9</em>, <em>Casp3</em>, and <em>Parp2</em>, while upregulating anti-apoptotic genes <em>Bcl2</em> and <em>Bcl-xl</em>. CSA demonstrates cardioprotective efficacy against DOX-induced cardiotoxicity by modulating ROS-inflammation-related oxidative and inflammatory pathways, supporting its potential for functional food applications.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107197"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of GABA-enriched rice germ extract fermented by Lactobacillus brevis BJ20 on stress symptoms and related biomarkers in adults: a randomized, double-blind, placebo-controlled trial 由短乳杆菌BJ20发酵的富含gaba的大米胚芽提取物对成人应激症状和相关生物标志物的影响：一项随机、双盲、安慰剂对照试验

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-01-30 DOI: 10.1016/j.jff.2026.107183

Ye Li Lee , Kyoung-min Rheu , Sang Yeoup Lee

{"title":"Effects of GABA-enriched rice germ extract fermented by Lactobacillus brevis BJ20 on stress symptoms and related biomarkers in adults: a randomized, double-blind, placebo-controlled trial","authors":"Ye Li Lee , Kyoung-min Rheu , Sang Yeoup Lee","doi":"10.1016/j.jff.2026.107183","DOIUrl":"10.1016/j.jff.2026.107183","url":null,"abstract":"<div><div>Stress is a growing public health concern, and nutritional approaches have gained increasing attention for improving mental well-being. Fermentation enhances the bioactive γ-aminobutyric acid (GABA) content of agric ultural materials. This 8-week, randomized, double-blind, placebo-controlled trial evaluated the efficacy and safety of a novel GABA-enriched rice germ extract (RG30), produced by <em>Lactobacillus brevis</em> BJ20 fermentation, in 80 adults with mild-to-moderate perceived stress. Participants received 500 mg/day RG30 (120 mg GABA) or placebo. RG30 supplementation significantly reduced Stress Response Inventory scores compared with placebo (adjusted mean difference: −5.39; 95% CI: −10.25, −0.54; <em>p</em> = 0.035) and improved quality of life (EuroQol VAS, <em>p</em> ≤ 0.002) after 8 weeks. Among secondary outcomes, plasma serotonin levels showed relative preservation in the RG30 group with placebo (<em>p</em> = 0.036). No serious adverse events occurred. RG30 represents a safe, fermented, plant-derived functional ingredient that alleviates stress symptoms and enhances quality of life.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107183"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-chain fatty acids attenuate obesity-induced oxidative stress by regulating the MUFA/PUFA ratio in phospholipids 短链脂肪酸通过调节磷脂中MUFA/PUFA比例来减轻肥胖诱导的氧化应激

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-18 DOI: 10.1016/j.jff.2026.107223

Yubin Li , Jia Zhang , Yuqiang Li , Yuming Su , Nan Li

引用次数: 0

Probiotic mixture enhances spatial memory via gut-brain axis modulation in mice 益生菌混合物通过肠-脑轴调节增强小鼠空间记忆

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI: 10.1016/j.jff.2026.107211

Bao-Hong Lee , I-Hong Pan , Kuei-Chang Li , Shu-Chi Cho , Cheng-Hao Huang , Wei-Hsuan Hsu

{"title":"Probiotic mixture enhances spatial memory via gut-brain axis modulation in mice","authors":"Bao-Hong Lee , I-Hong Pan , Kuei-Chang Li , Shu-Chi Cho , Cheng-Hao Huang , Wei-Hsuan Hsu","doi":"10.1016/j.jff.2026.107211","DOIUrl":"10.1016/j.jff.2026.107211","url":null,"abstract":"<div><div>Probiotics modulate gut-brain communication and influence cognitive function. This study investigated the effects of a probiotic mixture, Pro-neuron, on spatial memory and gut microbiota in healthy mice. Male C57BL/6 mice were divided into three groups: blank control, positive control (PC, gamma-aminobutyric acid), and Pro-neuron. After four weeks of oral administration, spatial memory was assessed using the Y-maze test, and gut microbiota composition was analyzed via 16S rRNA sequencing. Pro-neuron significantly enhanced memory performance, as indicated by increased alternation behavior. Immunohistochemistry showed elevated hippocampal expression of brain-derived neurotrophic factor (BDNF), while acetylcholinesterase (AChE) levels remained unchanged. Pro-neuron also enriched beneficial microbiota taxa, including <em>Bifidobacterium</em>, <em>Lactobacillus</em>, and <em>Muribaculum</em>, which are associated with neuroactive metabolite production, including short-chain fatty acids. These findings suggest that Pro-neuron may enhance spatial memory by modulating gut microbiota and promoting hippocampal BDNF expression, supporting its potential as a cognitive health supplement.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"138 ","pages":"Article 107211"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0