Journal of Functional Foods最新文献_第3页

Mechanistic study on the effects of acerola cherry concentrate on LPS-induced IEC-6 cell injury and Vinorelbine-induced immune function damage in zebrafish 针叶樱桃浓缩液对lps诱导的斑马鱼IEC-6细胞损伤和长春瑞滨诱导的免疫功能损伤的影响机制研究

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-27 DOI: 10.1016/j.jff.2025.106949

Huilin Zhu , Dingli Wang , Jindan Han , Ling Tong , Yanqing Li , Cuifeng Cao , Lalai Zikela , Zhuoli Yu , Xinyao Li , Hui Zhou , Songtao Li , Libing Chen , Qiang Han

{"title":"Mechanistic study on the effects of acerola cherry concentrate on LPS-induced IEC-6 cell injury and Vinorelbine-induced immune function damage in zebrafish","authors":"Huilin Zhu , Dingli Wang , Jindan Han , Ling Tong , Yanqing Li , Cuifeng Cao , Lalai Zikela , Zhuoli Yu , Xinyao Li , Hui Zhou , Songtao Li , Libing Chen , Qiang Han","doi":"10.1016/j.jff.2025.106949","DOIUrl":"10.1016/j.jff.2025.106949","url":null,"abstract":"<div><div>Acerola cherry (<em>Malpighia emarginata</em> DC.), renowned for its exceptional natural vitamin C (VC) and bioactive compounds, exhibits antioxidant, anti-inflammatory, and immunomodulatory properties. This study systematically investigated the protective effects of Acerola Cherry Concentrate (AC) against LPS-induced oxidative injury in IEC-6 cells in vitro and vinorelbine-triggered immune dysfunction in zebrafish in vivo. AC significantly enhanced catalase and superoxide dismutase activities while reducing malondialdehyde (MDA) levels in IEC-6 cells, outperforming synthetic VC in mitigating LPS-induced oxidative stress. Mechanistically, AC suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and modulated the NF-κB/TLR4/MyD88 signaling pathway, attenuating inflammatory responses. In zebrafish models, AC increased neutrophil and macrophage recruitment but showed limited enhancement of macrophage phagocytosis. Notably, the synergistic interaction between natural VC and other bioactive components in AC contributed to its synergy-enhanced efficacy. These findings underscore AC's potential as a multifunctional agent for combating oxidative stress and inflammation, offering mechanistic insights for its application in functional foods.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106949"},"PeriodicalIF":3.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary fibre and weaning age influence morphology, mucin distribution, and immunological traits in the porcine colon 饲粮纤维和断奶日龄影响猪结肠形态、粘蛋白分布和免疫特性

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-27 DOI: 10.1016/j.jff.2025.106947

Xiaoxiao Yu , Beatriz Martínez Vallespín , Łukasz Grześkowiak , Hermann-Josef Rothkötter , Wilfried Vahjen , Eva-Maria Saliu , Jürgen Zentek , Johannes Schulze Holthausen

{"title":"Dietary fibre and weaning age influence morphology, mucin distribution, and immunological traits in the porcine colon","authors":"Xiaoxiao Yu , Beatriz Martínez Vallespín , Łukasz Grześkowiak , Hermann-Josef Rothkötter , Wilfried Vahjen , Eva-Maria Saliu , Jürgen Zentek , Johannes Schulze Holthausen","doi":"10.1016/j.jff.2025.106947","DOIUrl":"10.1016/j.jff.2025.106947","url":null,"abstract":"<div><div>This study investigated the effects of dietary fibre type, particle size, and weaning age on colonic morphology, mucin distribution, and immune function in piglets. Ninety-four piglets were assigned to groups based on weaning age (28 ± 2 days, conventional weaning, CW; 42 ± 2 days, late weaning, LW) and fed diets with hay or sugar beet pulp (SBP, fine or coarse particles). After two weeks, tissue samples were collected. SBP enhanced the expression of IL-10 (<em>P =</em> 0.001) and GPR43 mRNA (<em>P</em> = 0.029), while it enhanced mucin presence in LW piglets (<em>P</em> < 0.01). In LW piglets, coarse particles increased crypt area (<em>P</em> = 0.012), and fine particles increased immune cell infiltration. Weaning age also affected crypt morphology and goblet cell numbers, with LW piglets showing deeper crypts (<em>P</em> < 0.001). Results suggest that fibre type, particle size, and weaning age significantly influence gut health and immunity.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106947"},"PeriodicalIF":3.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Water extracts of Pueraria thomsonii Radix ameliorates alcoholic liver disease via PI3K/AKT and NOX4/ROS pathways” [J. Funct. Foods 110 (2023) 105830] “葛根水提取物通过PI3K/AKT和NOX4/ROS途径改善酒精性肝病”的更正[J]。功能。食品110 (2023)105830]

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-26 DOI: 10.1016/j.jff.2025.106934

Kai Yang , Xiaofei Zhang , Ding Liu , Sihua Wen , Yanan Wu , Taotao Li , Tiantian Tang , Yujiao Wang , Ting Zou , Chongbo Zhao , Jing Sun

引用次数: 0

Gastrodia elata blume (Gastrodiae Rhizoma, Tianma) extract for cognitive enhancement in middle-aged adults with normal or mild impairment: A randomized, double-blind, placebo-controlled clinical trial 天麻天麻提取物对正常或轻度认知障碍中年人的认知增强作用：一项随机、双盲、安慰剂对照临床试验

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-23 DOI: 10.1016/j.jff.2025.106942

Hyungsun Jun , Junghan Lee , Byung Ouk Park , Ryeo Won Kim , Jungtae Leem , Kang-Beom Kwon

{"title":"Gastrodia elata blume (Gastrodiae Rhizoma, Tianma) extract for cognitive enhancement in middle-aged adults with normal or mild impairment: A randomized, double-blind, placebo-controlled clinical trial","authors":"Hyungsun Jun , Junghan Lee , Byung Ouk Park , Ryeo Won Kim , Jungtae Leem , Kang-Beom Kwon","doi":"10.1016/j.jff.2025.106942","DOIUrl":"10.1016/j.jff.2025.106942","url":null,"abstract":"<div><div>Early attention to cognitive impairment is vital. This 12-week, randomized, double-blind, placebo-controlled trial investigated the efficacy and safety of <strong>Gastrodia elata Blume (<em>G. elata</em>)</strong> in 100 participants aged 40–65 years with normal or impaired cognition. Participants received daily tablets, with primary outcome measured by the <strong>Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-cog)</strong>. Secondary outcomes included K-MMSE, BDNF, amyloid β, and TAS. After 12 weeks, the <strong><em>G. elata</em></strong> group significantly improved <strong>ADAS-cog</strong> total scores (−2.15 ± 2.22) compared to the placebo group (−0.92 ± 2.03). Improvements were also noted in K-MMSE total score, BDNF, and TAS, but not amyloid β. No serious adverse events were observed. These results suggest that <strong><em>G. elata</em></strong> extract safely and effectively improved cognitive function in middle-aged adults with normal or mild cognitive impairment, making it a promising agent for cognitive decline prevention and management.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106942"},"PeriodicalIF":3.8,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration 三氯蔗糖对角质细胞增殖的影响及其在表皮再生中的作用的细胞机制

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-21 DOI: 10.1016/j.jff.2025.106944

Pimngeon Chatkul , Wilasinee Satianrapapong , Wanapas Wachiradejkul , Nichakorn Thampathomwong , Tadhi Sucharitakul , Pichayapa Sukmak , Pichamon Sirilar , Pimpitcha Lertkiatdamrong , Jangkanipa Chatsiriwech , Natcha Thanasumpunth , Priyavudh Herabutya , Apiwan Arinno , Pawin Pongkorpsakol

{"title":"Cellular mechanisms underlying the effects of sucralose on keratinocyte proliferation and its contributing roles in epidermal regeneration","authors":"Pimngeon Chatkul , Wilasinee Satianrapapong , Wanapas Wachiradejkul , Nichakorn Thampathomwong , Tadhi Sucharitakul , Pichayapa Sukmak , Pichamon Sirilar , Pimpitcha Lertkiatdamrong , Jangkanipa Chatsiriwech , Natcha Thanasumpunth , Priyavudh Herabutya , Apiwan Arinno , Pawin Pongkorpsakol","doi":"10.1016/j.jff.2025.106944","DOIUrl":"10.1016/j.jff.2025.106944","url":null,"abstract":"<div><div>The main objectives of this study are to investigate the effects of sucralose on skin wound healing acceleration, UVB-induced epidermal epithelial damage, and to identify its underlying mechanisms of action. Sucralose significantly increased wound healing with an EC<sub>50</sub> of 30.47 μM, a maximal effect being observed at 100 μM. In addition, sucralose failed to induce wound healing in mitomycin C-primed HaCaT cell monolayers. Sucralose significantly increased keratinocyte proliferation and prevented UVB-induced cell death. These effects were suppressed by inhibitions of protein kinase A (PKA), AMP-activated protein kinase (AMPK), sirtuin-1 (SIRT-1), and extracellular signal-regulated kinase (ERK). Sucralose-induced AMPK phosphorylation was fully abolished by pretreatment with a PKA inhibitor. Sucralose-induced ERK phosphorylation was suppressed by inhibitors of AMPK and SIRT-1. Effect of sucralose-induced SIRT-1 activity was attenuated by PKA and AMPK inhibitor. Sucralose promotes wound healing and reduces keratinocyte death after UVB exposure by increasing cell proliferation via PKA/AMPK/SIRT-1/ERK-dependent mechanisms.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106944"},"PeriodicalIF":3.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coenzyme Q0, a quinone derivative from Antrodia camphorata, triggered ROS-mediated cytoprotective autophagy in human promyelocytic leukemia cells 辅酶Q0是一种从樟树中提取的醌衍生物，在人早幼粒细胞白血病细胞中引发ros介导的细胞保护性自噬

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-17 DOI: 10.1016/j.jff.2025.106939

Hsin-Ling Yang , Pei-Yuan Chiu , Hui-Jye Chen , Sudhir Pandey , Yue-Tong Chen , Siang-Jyun Chen , Yu-Lyu Yeh , Hsueh-Wei Chang , Jhih-Hsuan Hseu , You-Cheng Hseu

{"title":"Coenzyme Q0, a quinone derivative from Antrodia camphorata, triggered ROS-mediated cytoprotective autophagy in human promyelocytic leukemia cells","authors":"Hsin-Ling Yang , Pei-Yuan Chiu , Hui-Jye Chen , Sudhir Pandey , Yue-Tong Chen , Siang-Jyun Chen , Yu-Lyu Yeh , Hsueh-Wei Chang , Jhih-Hsuan Hseu , You-Cheng Hseu","doi":"10.1016/j.jff.2025.106939","DOIUrl":"10.1016/j.jff.2025.106939","url":null,"abstract":"<div><div>Our previous investigation revealed that Coenzyme Q<sub>0</sub> (CoQ<sub>0</sub>), a quinone derivative from <em>Antrodia camphorata</em>, triggered ROS-dependent voltage-dependent anion channel 1 (VDAC-1)-mediated apoptosis in human promyelocytic leukemia (HL-60) cells. This research examined the efficacies of CoQ<sub>0</sub> (0–7 μM) on autophagy induction and the interaction between apoptosis and autophagy in HL-60 cells. CoQ<sub>0</sub>-triggered autophagy was associated with LC3-I/II accumulation, p62/SQSTM1 activation, autolysosome AVOs formation, ATG4B inhibition, ATG5/ATG7 expression, and Beclin-1/Bcl-2 dysregulation in HL-60 cells. Additionally, antioxidant <em>N</em>-acetylcysteine (NAC) prevented CoQ<sub>0</sub>-induced autophagy in HL-60 cells. Surprisingly, CoQ<sub>0</sub>-triggered cell death was enhanced by autophagy inhibitor 3-MA or CQ, indicating that autophagy acts as a survival mechanism. Additionally, autophagy suppression <em>via</em> 3-MA and/or CQ inhibitors strengthened the CoQ<sub>0</sub>-induced ROS generation and apoptosis (caspase-3 activation). Apoptosis suppression by caspase inhibitor <em>Z</em>-VAD-FMK or VDAC-1 silencing prevented the CoQ<sub>0</sub>-enhanced autophagy in HL-60 cells. Therefore, CoQ<sub>0</sub> together with autophagy inhibitors could be a potential therapy for human promyelocytic leukemia.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106939"},"PeriodicalIF":3.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant peptides (FYDQ and FVEG) derived from cricket (Acheta domesticus) protein hydrolysate enhance photoprotection and inhibit apoptosis in UVB-irradiated HaCaT keratinocytes cells 从蟋蟀（Acheta domesticus）蛋白水解物中提取的抗氧化肽（FYDQ和FVEG）增强了uvb照射下HaCaT角质形成细胞的光保护作用并抑制了细胞凋亡

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-14 DOI: 10.1016/j.jff.2025.106940

Ratasark Summart , Natteewan Udomsil , Sumeth Imsoonthornruksa , Mariena Ketudat-Cairns

{"title":"Antioxidant peptides (FYDQ and FVEG) derived from cricket (Acheta domesticus) protein hydrolysate enhance photoprotection and inhibit apoptosis in UVB-irradiated HaCaT keratinocytes cells","authors":"Ratasark Summart , Natteewan Udomsil , Sumeth Imsoonthornruksa , Mariena Ketudat-Cairns","doi":"10.1016/j.jff.2025.106940","DOIUrl":"10.1016/j.jff.2025.106940","url":null,"abstract":"<div><div>Antioxidant peptides are extensively used in food, pharmaceuticals, and cosmetics. Two tetrapeptides, Phe-Tyr-Asp-Gln (FYDQ) and Phe-Val-Glu-Gly (FVEG), were previously identified from cricket (<em>Acheta domesticus</em>) protein hydrolysate. Molecular docking of these tetrapeptides showed high binding affinity to antioxidant-related proteins: Keap1, Superoxide dismutase (SOD), Catalase (CAT), GR (Glutathione reductase), and Glutathione peroxidase (GPx). In this study, FYDQ and FVEG were selected and evaluated for their protective effect against oxidative damage induced by UVB irradiation of human keratinocytes cells (HaCaT). Pretreatment with 100 μg/mL of each tetrapeptide before UVB irradiation resulted in intracellular photoprotective activity by reducing reactive oxygen species (ROS). These were accumulated by approximately two-fold compared to the UVB-irradiated HaCaT cells and restored the activity of antioxidant enzymes. Additionally, pretreatment with the tetrapeptides prior to UVB irradiation inhibited cellular apoptosis by approximately 30 % compared to the UVB-irradiated group. This was achieved by reducing the loss of mitochondrial membrane potential (MMP) by about 40 % and repressing the expression of cleaved caspase-3 and BAX by about two-fold compared to the UVB-irradiated group. FVDQ and FVEG demonstrate protective effects against UVB-induced oxidative damage through ROS scavenging leading to attenuated cellular apoptosis. Both tetrapeptides might be utilized as antioxidant components in sunscreen formulations or anti-photoaging cosmetic products.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106940"},"PeriodicalIF":3.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue: Valorization of regional foods for the improvement of health and well-being of consumers 特刊：为改善消费者的健康和福祉而使区域食品增值

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-12 DOI: 10.1016/j.jff.2025.106941

Elena Barbieri , Sauro Vittori

引用次数: 0

Polysaccharide from natural Ophiocordyceps sinensis alleviated dextran sulfate sodium-induced colitis through modulating sIgA formation and gut microbiota 天然冬虫夏草多糖通过调节sIgA的形成和肠道菌群减轻葡聚糖硫酸钠诱导的结肠炎

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-12 DOI: 10.1016/j.jff.2025.106921

Shuping Chen , Junqiao Wang , Qiuyue Fang , Nan Dong , Steve W. Cui , Shaoping Nie

{"title":"Polysaccharide from natural Ophiocordyceps sinensis alleviated dextran sulfate sodium-induced colitis through modulating sIgA formation and gut microbiota","authors":"Shuping Chen , Junqiao Wang , Qiuyue Fang , Nan Dong , Steve W. Cui , Shaoping Nie","doi":"10.1016/j.jff.2025.106921","DOIUrl":"10.1016/j.jff.2025.106921","url":null,"abstract":"<div><div>Polysaccharide from natural <em>Ophiocordyceps sinensis</em> (NCSP-50) significantly modulated the gut microbiota composition and elevated the levels of sIgA in dextran sodium sulfate (DSS)-induced colitis mice. However, the in-depth mechanism remains unknown. Therefore, this study aimed to investigate how NCSP-50 influenced sIgA formation and the role of gut microbiota in colitis mice. Results showed that administration of NCSP-50 dramatically improved the expressions of IgA, pIgR and AID, the key factors of sIgA formation in colitis mice. NCSP-50 significantly decreased the quantity of dendritic cells and mRNA expression of <em>tlr-2</em>, <em>tlr-4</em> and <em>tlr-6</em>, <em>april</em> and <em>raldh-1</em>, while improved the expression of <em>vapc2</em>, <em>raldh-2</em> and <em>iNOS</em>, indicating the involvement in T-cell independent pathway. NCSP-50 reduced the amount of CD4<sup>+</sup> T cells, and adjusted the expression of nuclear transcription factors of T cells subsets and the release of IL-13, indicating the involvement in T-cell dependent pathway. In addition, fecal microbiota transplantation revealed that gut microbiota from mice administrated with NCSP-50 obviously alleviated colitis-related symptoms and increased the concentration of sIgA, whereas antibiotic cocktails treatments abolished those effects. In conclusion, NCSP-50 attenuated DSS-induced colitis through intestinal microbiota and promoting the T-cell dependent and independent sIgA secretion influenced by gut microbiota.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"131 ","pages":"Article 106921"},"PeriodicalIF":3.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inonotus obliquus (Chaga) attenuates folic acid-induced renal interstitial fibrosis by inhibiting epithelial-mesenchymal transition through modulating macrophages and T cells activation and interaction 斜棘棘猴（Chaga）通过调节巨噬细胞和T细胞的活化和相互作用，抑制上皮-间质转化，从而减轻叶酸诱导的肾间质纤维化

IF 3.8 2区农林科学

Journal of Functional Foods Pub Date : 2025-06-11 DOI: 10.1016/j.jff.2025.106936

Yaling Zhang , Yueling Peng , Xinyu Wang , Rui Wang , Xingwei Liu , Kaiyun Li , Hui Liao , Rongshan Li

{"title":"Inonotus obliquus (Chaga) attenuates folic acid-induced renal interstitial fibrosis by inhibiting epithelial-mesenchymal transition through modulating macrophages and T cells activation and interaction","authors":"Yaling Zhang , Yueling Peng , Xinyu Wang , Rui Wang , Xingwei Liu , Kaiyun Li , Hui Liao , Rongshan Li","doi":"10.1016/j.jff.2025.106936","DOIUrl":"10.1016/j.jff.2025.106936","url":null,"abstract":"<div><div>Renal interstitial fibrosis (RIF), with extracellular matrix (ECM) deposition in the renal interstitium as the pathological feature, is triggered by inflammation and is associated with poor outcomes in chronic kidney disease (CKD). Macrophages and T cells are the main inflammatory effectors in RIF. Inonotus obliquus (Chaga), used as food and medicine, was recently found to protect against CKD in our previous study. However, its effect on RIF remains unclear. In this study, C57BL/6 mice were intraperitoneally injected with folic acid (FA) to induce RIF, followed by intragastric administration of chaga for 14 days. The dose-dependent efficacy of 100, 200, and 300 mg/kg chaga in improving RIF was first verified using histopathology. The results revealed that chaga attenuated RIF, and the protective effect was most significant at a dose of 300 mg/kg. In the subsequent study, dynamic courses of chaga at 300 mg/kg reduced renal tubular damage and decreased collagen deposition area were observed on days 3, 7, and 14. In addition, single-cell RNA sequencing and immunohistochemistry studies demonstrated that chaga inhibited macrophage and T cell aggregation in fibrotic kidneys, and further normalized the communication between macrophages and T cells. The latter was based on our analysis that chaga inhibited FA-induced CD86-CD28 ligand-receptor interactions but restored macrophage histocompatibility complex class 1 and CD8 levels between renal macrophages and T cells. In conclusion, chaga effectively suppressed RIF in FA-induced mice by modulating macrophage and T cell activation and interaction.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"130 ","pages":"Article 106936"},"PeriodicalIF":3.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0