Journal of Functional Foods最新文献_第10页

The effects of fermentation by cholesterol-lowering fungi on the functional components and bioactive properties of kiwifruit pomace 降胆固醇真菌发酵对猕猴桃果渣功能成分及生物活性的影响

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-01-01 Epub Date: 2026-01-06 DOI: 10.1016/j.jff.2025.107128

Ang Liu , Jiang-yong Hong , Fei Huang , Bo-yu Dong , Qiu-ping Yao , De-quan Zhu

{"title":"The effects of fermentation by cholesterol-lowering fungi on the functional components and bioactive properties of kiwifruit pomace","authors":"Ang Liu , Jiang-yong Hong , Fei Huang , Bo-yu Dong , Qiu-ping Yao , De-quan Zhu","doi":"10.1016/j.jff.2025.107128","DOIUrl":"10.1016/j.jff.2025.107128","url":null,"abstract":"<div><div>Kiwifruit pomace, a processing byproduct with lipid metabolism-regulating potential, is often discarded because of its low bioavailability. In this study, fungal fermentation, which is an efficient bioprocessing strategy, was applied to increase the contents of its active components and its bioavailability. Seven cholesterol-lowering fungal strains isolated from fermented foods—<em>Talaromyces</em> sp. FR, <em>Aspergillus</em> sp. C1YC, <em>Fusarium solani</em> A4YC, <em>Alternaria alstroemeriae</em> C2SLB, <em>Monascus purpureus</em> HQM, <em>Penicillium rubens</em> C3SLB, and <em>Penicillium chrysogenum</em> C2YC—were used for pomace fermentation. The contents of flavonoids, total phenolics, and soluble dietary fibre significantly increased (<em>P</em> < 0.05), with the greatest increases (1.94-, 1.42-, and 1.31-fold, respectively) observed in the HQM group. All the strains and fermented pomace extracts exhibited considerable cholesterol-degrading ability, reaching 74.79 % with the HQM suspension. Extracts of C1YC and C3SLB showed prominent antioxidant capacity, whereas the HQM extract strongly bound bile salts. α-Glucosidase activity and α-amylase activity were inhibited by the C2YC and HQM suspensions (84.84 % and 83.01 %, respectively). Compared with the control, the FR-fermented pomace adsorbed 6.01 times more fat. This study is the first to report direct cholesterol degradation by <em>Talaromyces</em> sp., <em>Fusarium solani</em>, and <em>Alternaria alstroemeriae</em>, confirming that fungal fermentation increases the value of kiwifruit pomace.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"136 ","pages":"Article 107128"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity effects of fucoxanthin and conjugated linoleic acid complex liposomes in high-fat diet mice through regulation of AMPK signaling pathway 岩藻黄素和共轭亚油酸复合脂质体通过调节AMPK信号通路对高脂饮食小鼠的抗肥胖作用

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-01-01 Epub Date: 2026-01-06 DOI: 10.1016/j.jff.2025.107142

Jiyan Sun , Yanxiaofan Sun , Yuxin Shi , Xuhui Lei , Zhiying Xu , Yichao Ma , Shu Liu , Yunhai He , Qiukuan Wang , Dandan Ren

{"title":"Anti-obesity effects of fucoxanthin and conjugated linoleic acid complex liposomes in high-fat diet mice through regulation of AMPK signaling pathway","authors":"Jiyan Sun , Yanxiaofan Sun , Yuxin Shi , Xuhui Lei , Zhiying Xu , Yichao Ma , Shu Liu , Yunhai He , Qiukuan Wang , Dandan Ren","doi":"10.1016/j.jff.2025.107142","DOIUrl":"10.1016/j.jff.2025.107142","url":null,"abstract":"<div><div>Solving the problem of obesity has been globally recognized as a crucial public health challenge. Recent studies have shown that fucoxanthin and conjugated linoleic acid could work together to exert an anti-obesity effect. In this study, sodium alginate- and chitosan-modified fucoxanthin and conjugated linoleic acid complex liposome (S-C-L) was prepared and characterized. The anti-obesity effect and mechanism of action of S-C-L were evaluated by establishing an obese mouse model. The results indicated that S-C-L reduced the weight and fat content of obese mice and improved their oxidative stress state. It regulated the expression levels of mRNA and protein by activating the AMPK signaling pathway, thereby enhancing energy expenditure and inhibiting the differentiation of adipocytes, confirming its anti-obesity properties. Comprehensive analysis indicated that S-C-L had broad application potential in the development of anti-obesity foods and health products.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"136 ","pages":"Article 107142"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoencapsulated gamma-oryzanol enhances fibroblast proliferation, migration, and reduces oxidative stress in H2O2-induced cellular aging model 在h2o2诱导的细胞衰老模型中，纳米包埋γ -米甲醇增强成纤维细胞增殖、迁移和降低氧化应激

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2026-01-01 Epub Date: 2025-12-15 DOI: 10.1016/j.jff.2025.107130

Priya Yadav , Janani Sankar , Christoph Mora , Shengyu Mu , Nancy J. Rusch , Fumiya Watanabe , Gregory Guisbiers , Sankar Devarajan

{"title":"Nanoencapsulated gamma-oryzanol enhances fibroblast proliferation, migration, and reduces oxidative stress in H2O2-induced cellular aging model","authors":"Priya Yadav , Janani Sankar , Christoph Mora , Shengyu Mu , Nancy J. Rusch , Fumiya Watanabe , Gregory Guisbiers , Sankar Devarajan","doi":"10.1016/j.jff.2025.107130","DOIUrl":"10.1016/j.jff.2025.107130","url":null,"abstract":"<div><div>This study investigates the impact of NanoEncapsulated Gamma Oryzanol (NEGO) on H<sub>2</sub>O<sub>2</sub>-induced NIH/3T3 fibroblasts and the enzymes associated with aging. NEGO was prepared using high-speed emulsification and characterized by UV–visible spectroscopy, dynamic light scattering (DLS), zeta potential, scanning and transmission electron microscopy techniques (SEM and TEM). Its antioxidant activity and enzyme inhibition (elastase, collagenase, hyaluronidase, and tyrosinase) were assessed, and <em>in-silico</em> docking was used to determine the binding propensity of Gamma Oryzanol (GO) with the enzymes. NEGO showed a remarkable absorption peak of GO at 326 nm, average size distribution of 162 nm (PDI: 0.2), large zeta potential (−51 mV), smooth, and spherical morphology. It showed a strong antioxidant effect and a dose-dependent inhibition of enzymes, and molecular docking confirmed the high binding affinity of GO (−7.0 to −8.1 kcal/mol) with the enzymes. NEGO enhanced cell viability, lowered reactive oxygen Species (ROS), and accelerated fibroblast migration. These findings highlight NEGO's promise as a natural, food-derived approach to mitigate ROS-induced cellular damage.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"136 ","pages":"Article 107130"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhubarb polysaccharides alleviate hypertriglyceridemia-induced acute pancreatitis and regulate gut microbiota-mediated tryptophan metabolism 大黄多糖减轻高甘油三酯血症诱导的急性胰腺炎和调节肠道微生物介导的色氨酸代谢

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1016/j.jff.2025.107104

Ya Chen , Wen-Wen Zhang , Yun-Yun Mao , Gang-Ao Hu , Xin-Xin Chen , Yi-Lin Zhou , Xiao-Long Chen , Hong Wang , Bin Wei , Jian-Feng Tu

{"title":"Rhubarb polysaccharides alleviate hypertriglyceridemia-induced acute pancreatitis and regulate gut microbiota-mediated tryptophan metabolism","authors":"Ya Chen , Wen-Wen Zhang , Yun-Yun Mao , Gang-Ao Hu , Xin-Xin Chen , Yi-Lin Zhou , Xiao-Long Chen , Hong Wang , Bin Wei , Jian-Feng Tu","doi":"10.1016/j.jff.2025.107104","DOIUrl":"10.1016/j.jff.2025.107104","url":null,"abstract":"<div><div><em>Rheum tanguticum</em> plays a key role in treating acute pancreatitis, though its bioactive components and mechanisms remain unclear. This study investigates the structural characteristics and therapeutic effects of rhubarb polysaccharides (RP) in a mouse model of hypertriglyceridemia-induced acute pancreatitis (HTGAP). The isolated polysaccharide, (26 kDa, primarily glucose and arabinose) significantly reduced serum amylase and lipase levels, improved pancreatic and intestinal histopathology and upregulated tight junction proteins Zonula Occludens-1 and Occludin. High throughput 16S rRNA sequencing demonstrated that RP supplementation modulated gut microbiota by enriching beneficial genera (e.g., <em>Lactobacillus</em> and <em>Akkermansia</em>) while reducing pathogenic genera (<em>Lachnoclostridium</em>, <em>Desulfovibrio</em>). Untargeted metabolomics revealed alterations in microbial metabolites, particularly in the tryptophan metabolism. Integrated analysis revealed significant microbiota-metabolite correlations. These findings suggest that RP improve HTGAP by restoring intestinal barrier integrity and regulating gut microbiota-mediated tryptophan metabolism, highlighting its potential as a therapeutic agent.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107104"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of liposomal multinutrients versus non-liposomal comparators in a randomized crossover trial 一项随机交叉试验中多营养素脂质体与非脂质体比较物的药代动力学

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1016/j.jff.2025.107103

Young Min Woo , Keun Woo Lee , Andre Kim

{"title":"Pharmacokinetics of liposomal multinutrients versus non-liposomal comparators in a randomized crossover trial","authors":"Young Min Woo , Keun Woo Lee , Andre Kim","doi":"10.1016/j.jff.2025.107103","DOIUrl":"10.1016/j.jff.2025.107103","url":null,"abstract":"<div><div>Liposomal delivery systems are utilized to enhance the bioavailability of nutrients. In this randomized, double-blind, two-period crossover clinical trial, healthy Korean adults (<em>n</em> = 20) were enrolled to assess the absorption of liposomal multinutrients compared to composition-matched non-liposomal comparators. Two distinct liposomal multinutrient formulations (LMN-1 and LMN-2) were compared with two corresponding non-liposomal multinutrient products (NLMN-1 and NLMN-2). Pharmacokinetic (PK) parameters—including C_max, T_max, and incremental area under the curve (iAUC)—were analyzed for vitamin B3, vitamin C, zinc, and iron. Vitamin C and zinc concentrations were significantly higher from 1 to 8 h and 0–6 h, respectively, while iron concentrations were elevated at most time points for the liposomal group. No clinically significant adverse events were observed during the study period. These findings indicate that liposomal multinutrients improve nutrient absorption and demonstrate good tolerability in humans. The results support the application of liposomal formulations in functional foods as a strategy to enhance nutrient delivery.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107103"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive peptides as functional food ingredients: Production, mechanisms of action, market trends, and future perspectives with emphasis on andean crops 生物活性肽作为功能性食品成分：生产、作用机制、市场趋势和未来展望，重点是安第斯作物

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-22 DOI: 10.1016/j.jff.2025.107109

Gabriela Ibieta , Jimena Ortiz-Sempértegui , J. Mauricio Peñarrieta , Javier A. Linares-Pastén

{"title":"Bioactive peptides as functional food ingredients: Production, mechanisms of action, market trends, and future perspectives with emphasis on andean crops","authors":"Gabriela Ibieta , Jimena Ortiz-Sempértegui , J. Mauricio Peñarrieta , Javier A. Linares-Pastén","doi":"10.1016/j.jff.2025.107109","DOIUrl":"10.1016/j.jff.2025.107109","url":null,"abstract":"<div><div>The development of bioactive peptides is of great interest in the food and pharmaceutical industries because of their positive impact on health. However, peptide's bioavailability depends on their stability along the gastrointestinal tract and their ability to be transported to targets located in peripheral organs, whose mechanisms are still poorly understood. However, several studies, mainly <em>in vitro</em> and <em>in vivo</em>, have shown antimicrobial, antitumoral, antihypertensive, anti-inflammatory, antidiabetic, and antioxidative effects. Indeed, some bioactive peptides are already on the global market. One of the current and future perspectives includes the development of novel bioactive peptides from food plant proteins. In this regard, some grains and seeds, such as quinoa, maize, cañihua and tarwi, which were domesticated in the Andes, are attracting global interest due to their high nutritional value and potential as a source of novel bioactive peptides. Tarwi stands out for its high protein content compared to other legumes.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107109"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From traditional medicinal use to functional food: The pharmacological activity, hepatotoxicity mechanism and detoxification strategies of gardenia fructus 从传统药用到功能性食品：栀子的药理活性、肝毒性机制及解毒策略

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-06 DOI: 10.1016/j.jff.2025.107093

Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang

{"title":"From traditional medicinal use to functional food: The pharmacological activity, hepatotoxicity mechanism and detoxification strategies of gardenia fructus","authors":"Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang","doi":"10.1016/j.jff.2025.107093","DOIUrl":"10.1016/j.jff.2025.107093","url":null,"abstract":"<div><div>Gardenia fructus (GF), a medicinal and edible herb with over 2,000 years of use, possesses diverse bioactive compounds including iridoids, crocins, and organic acids. Herein, we summarize the ethnomedicinal applications, phytochemistry, pharmacological activities, and safety concerns. GF exhibits a wide range of pharmacological activities, notably hepatoprotective, renoprotective, and neuroprotective effects. However, dose-dependent hepatotoxicity and nephrotoxicity - primarily linked to genipin, a metabolite of geniposide - pose significant safety challenges. Mechanisms involve oxidative stress, mitochondrial dysfunction, bile acid dysregulation, and inflammatory cascade activation. The field lacks sufficient clinical translation, comprehensive bioactivity analysis, and stringent quality control. Future research should prioritize elucidating molecular toxicity pathways, developing quality control benchmarks include geniposide, and advancing clinical trials to balance efficacy with safety. This positions GF as a sustainable resource for nutraceuticals and functional foods, necessitating rigorous safety assessments for rational utilization.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107093"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleurotus eryngii extruded rice improved HFD/STZ-induced type 2 diabetic mice by activating PI3K/AKT signaling pathway and regulating gut microbiota 杏鲍菇膨化米通过激活PI3K/AKT信号通路和调节肠道菌群改善HFD/ stz诱导的2型糖尿病小鼠

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-08 DOI: 10.1016/j.jff.2025.107096

Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma

{"title":"Pleurotus eryngii extruded rice improved HFD/STZ-induced type 2 diabetic mice by activating PI3K/AKT signaling pathway and regulating gut microbiota","authors":"Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma","doi":"10.1016/j.jff.2025.107096","DOIUrl":"10.1016/j.jff.2025.107096","url":null,"abstract":"<div><div><em>P.eryngii</em> extruded rice (PEER) has been demonstrated to be a medium glycemic index food. To investigate its hypoglycemic potential, this study established a type 2 diabetic mouse model using streptozotocin (STZ) combined with a high-fat diet, followed by an 8 wk. dietary intervention. The results showed that, compared to the model control (MC) group, PEER intervention significantly lowered blood glucose, improved oral glucose tolerance (OGTT), reduced blood lipids (TC, TG, and LDL-C decreased by 18.22 %, 19.65 %, and 52.25 %, respectively) and insulin levels. In addition, PEER significantly upregulated the relative expression of IR, IRS-1, IRS-2, PI3K, and GSK-3β genes in the liver, altered the composition and structural proportion of gut microbiota in mice and notably increased the abundance of beneficial bacteria such as Akkermansia. In conclusion, PEER exhibited a significant hypoglycemic effect in type 2 diabetic mice, providing a theoretical basis for developing <em>P. eryngii</em>-based hypoglycemic products.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107096"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic properties of Lacticaseibacillus paracasei MJ4 and its protective effect on mice with inflammatory bowel disease: A potential probiotic resource 副干酪乳杆菌MJ4的益生菌特性及其对炎症性肠病小鼠的保护作用：一种潜在的益生菌资源

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jff.2025.107074

Jianlong Lao , Junmei Li , Lin Tan , Shuping Yan , Zhaohai Wen , Yuan Qiu , Huiming Tan , Zengyuan Liu , Yanhong Yong , Xiaoxi Liu , Xingbin Ma , Xianghong Ju , Youquan Li

{"title":"Probiotic properties of Lacticaseibacillus paracasei MJ4 and its protective effect on mice with inflammatory bowel disease: A potential probiotic resource","authors":"Jianlong Lao , Junmei Li , Lin Tan , Shuping Yan , Zhaohai Wen , Yuan Qiu , Huiming Tan , Zengyuan Liu , Yanhong Yong , Xiaoxi Liu , Xingbin Ma , Xianghong Ju , Youquan Li","doi":"10.1016/j.jff.2025.107074","DOIUrl":"10.1016/j.jff.2025.107074","url":null,"abstract":"<div><div>Intestinal inflammation in animals is a common intestinal disease, among which Crohn's disease (CD) and ulcerative colitis (UC) are the most common. However, probiotics are strain-specific, and there are significant differences in the mechanisms of action among different strains. In this study, <em>Lacticaseibacillus paracasei</em> MJ4 was isolated from the homemade yogurt of herdsmen in Qinghai Province, China. In vitro experiments demonstrated that MJ4 possesses excellent probiotic properties and safety profiles. In a murine model of colitis, MJ4 alleviated weight loss and colon shortening, and reduced the DAI score. After intervention with MJ4, the levels of pro-inflammatory factors (IL-1β, TNF-α, and IL-6) and histopathological scores decreased, whereas the level of IL-10 and the number of goblet cells increased significantly. Additionally, MJ4 rebalanced the intestinal flora composition in colitis mice by increasing the abundance of <em>Faecalibaculum</em> and <em>Alistipes</em>, and decreasing the abundance of <em>Bacteroides</em>, <em>Escherichia_Shigella</em>, and <em>Akkermansia</em>. Collectively, these findings indicate that L. <em>paracasei</em> MJ4 holds substantial potential for preventing colitis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107074"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145374606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and molecular mechanisms of novel wound healing potential peptides from eggshell membrane hydrolysates assisted by an integrated in silico and in vitro approach 从蛋壳膜水解物中鉴定新型创面愈合潜力肽及其分子机制——基于硅和体外集成方法的研究

IF 4 2区农林科学

Journal of Functional Foods Pub Date : 2025-12-01 Epub Date: 2025-11-21 DOI: 10.1016/j.jff.2025.107099

Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu

{"title":"Identification and molecular mechanisms of novel wound healing potential peptides from eggshell membrane hydrolysates assisted by an integrated in silico and in vitro approach","authors":"Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu","doi":"10.1016/j.jff.2025.107099","DOIUrl":"10.1016/j.jff.2025.107099","url":null,"abstract":"<div><div>The aim of this study was to demonstrate the wound-healing activity of novel peptides derived from the eggshell membranes (ESM) of fresh chicken eggs and to explore the underlying mechanisms using integrated <em>in silico</em> and <em>in vitro</em> approaches. The ESM hydrolysates prepared by papain (PapH) exhibited the highest antioxidant and wound-healing activity. Three novel peptides, KDFPCPM (P1), DKDGPFRLC (P2), and LVEKGDVAF (P3), were identified from PapH <em>via</em> peptidomics and subsequently synthesized. These synthetic peptides also displayed excellent antioxidant and wounding-healing effects, with P1 surpassed the other peptides. Molecular docking results indicated that P1 could form stable bonds with Keap1, a key antioxidant target identified through proteomics. Furthermore, <em>in vitro</em> experiments utilizing the Nrf2 inhibitor ML385 revealed that P1 from ESM promotes cell migration by activating the Keap1-mediated Nrf2/HO-1 signaling pathway, thereby enhancing the cellular antioxidant defense system. Collectively, these findings provided new insights for the high-value utilization of discarded ESM.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107099"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0