Polysaccharide from Ziziphus jujuba fruit protects ulcerative colitis mice via IL-33/ST2 signal Axis

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-05-11 DOI:10.1016/j.jff.2025.106839

Shiying Huang , Rui Wang , Xueying Lu , Pingli Mo , Jiazhen Wu , Zhonggui Li , Zheng-Quan Lai , Huimin Yu , Muxia Li , Jianping Chen

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Abstract

Jujube is a medicinal herb and daily food. Polysaccharides extracted from jujube possesses immune regulatory activity. In this study, we investigated whether jujube polysaccharides (JP) could ameliorate ulcerative colitis (UC), an inflammatory disease, and explore the potential mechanism of UC. We first extracted and characterized the structure JP, using dextran sulfate sodium-induced UC mice model to verify the pharmacological function. Research proved that JP treatment could ease UC mice symptoms and pathological injury. According to transcriptomics analysis, majority genes were enriched to cytokines-cytokines receptors signal pathway. Results showed that the increasing proinflammatory cytokines in UC mice colon were declined after JP intervention. Meanwhile, the receptor of interleukin-33 (IL-33), tumorigenicity 2 suppression (ST2) and downstream protein MYD88 expression in UC mice colon were corrected by JP intervention. As a result, according to immunohistochemistry detection, JP downregulated UC mice colon T cells expression (labeled by CD3+). We further employed flow cytometry to validate that JP could suppress CD4+ high expression on Jurkat cell stimulated by IL-33. In conclusion, JP could ameliorate UC mice symptoms, the mechanism of which was related to the regulation of IL-33/ST2 axis.

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酸枣多糖通过IL-33/ST2信号轴保护溃疡性结肠炎小鼠

枣是一种药材和日常食品。红枣多糖具有免疫调节作用。在这项研究中，我们研究了红枣多糖（JP）是否可以改善溃疡性结肠炎（UC），并探讨UC的可能机制。我们首先提取并表征了JP的结构，利用葡聚糖硫酸钠诱导UC小鼠模型验证其药理功能。研究证实，JP治疗可减轻UC小鼠的症状和病理损伤。转录组学分析显示，大部分基因富集于细胞因子-细胞因子受体信号通路。结果表明，JP干预后UC小鼠结肠促炎细胞因子的增加有所下降。同时，通过JP干预，UC小鼠结肠中白细胞介素-33 （IL-33）受体、致瘤性2抑制（ST2）和下游蛋白MYD88的表达得到纠正。结果，免疫组化检测显示，JP下调UC小鼠结肠T细胞表达（CD3+标记）。我们进一步利用流式细胞术验证了JP对IL-33刺激的Jurkat细胞CD4+高表达的抑制作用。综上所述，JP可改善UC小鼠的症状，其机制可能与调节IL-33/ST2轴有关。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.