Journal of Functional Foods最新文献

{"title":"Bergamot (Citrus bergamia) peel extract as new hypocholesterolemic agent modulating PCSK9 expression","authors":"Irene Ferrarese ,&nbsp;Maria Giovanna Lupo ,&nbsp;Ilaria Rossi ,&nbsp;Stefania Sut ,&nbsp;Francesca Loschi ,&nbsp;Pietro Allegrini ,&nbsp;Antonella Riva ,&nbsp;Nicola Ferri ,&nbsp;Stefano Dall'Acqua","doi":"10.1016/j.jff.2023.105724","DOIUrl":"https://doi.org/10.1016/j.jff.2023.105724","url":null,"abstract":"<div><p><em>Citrus bergamia</em> extracts have been studied for the management of hypercholesterolemia disorders. Up to now limited information is available concerning the activity of its main phytoconstituents towards the main targets of the cholesterol homeostasis. In the present study, the effects of bergamot peel extract and isolated constituents, namely glycosidic and non-glycosidic flavonoids, one coumarin and one limonoid on the low-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) were evaluated by using cultured HuH7 cell line. Furthermore, for the first time the effects of bergamot peel extract were studied to describe a potential hypolipidemic action. Significant differences were observed due to glycosylation and different substitution on flavanone moiety (<em>O</em>-methylation). Considering the thirteen isolated compounds, both naringenin-7-<em>O</em>-rutinoside (NA-rut) and apigenin-6,8-<em>C</em>-glicoside induced the expression of the LDLR while no effect was observed on PCSK9. However, hesperetin (HE) and its derivatives, hesperetin-7-<em>O</em>-glucoside and hesperetin-7-<em>O</em>-neohesperidoside (HE-glu, HE-neo) and eriodictyol (ER) showed a statin-like effect since a significant increase of both LDLR and PCSK9 expression were detected. Furthermore, bergamot peel extract (BE) firstly demonstrated a significant reduction of PCSK9 expression, indicating a potential adjuvant action to statins. BE, HE-neo, and NA-rut reduced intracellular sterols and the expression of PCSK9 transcription factor HNF1-α. BE also significantly improved the LDL uptake u Huh7 cells. Based on the present data, bergamot peel constituents can play a role in the management of hypercholesterolemia, on one hand they may produce an adjuvant action in combination with statins, while on the other hand they may have a statin-like effect. Additionally, BE may be a good candidate as an adjuvant to statins action.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105724"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3461684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sweet-inhibiting effects of gurmarin on intake during repeated acute and long-term sugar exposure: A behavioural analysis using an animal model","authors":"Raquel Rayo-Morales ,&nbsp;Antonio Segura-Carretero ,&nbsp;Nicolas Poirier ,&nbsp;Loïc Briand ,&nbsp;David Garcia-Burgos","doi":"10.1016/j.jff.2023.105743","DOIUrl":"10.1016/j.jff.2023.105743","url":null,"abstract":"<div><p>Excessive consumption of added sugars is linked to chronic diseases, such as obesity, diabetes, dyslipidaemia or cardiovascular disease; and public health is searching for new strategies to reduce it. Although plant-derived bioactive compounds with inhibitory properties of sweet taste like <em>Gymnema sylvestre</em> show the potential to reduce sugar intake acutely, their impact after repeated administration is unknown. Therefore, we examined the changes of single and repeated exposures of a <em>Gymnema sylvestre</em> constituent, gurmarin, in sweet beverage consumption and preference in a preclinical model. 24 Wistar rats (50 % females) were divided into experimental (gurmarin) and two control groups (gymnemic acids or phosphate buffer solutions) according to the substances orally applied. Then acceptance and preference tests with sugar were performed within (Experiment 1) and between sessions (Experiment 2). We found that administering gurmarin decreased sucrose intake significantly, even after multiple treatments, without rebound effects. These findings suggest that sweet taste suppressors could be an effective tool for reducing long-term sugar consumption when repeatedly administrated.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105743"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003432/pdfft?md5=b88d525084fe2c17e817eef7514284ab&pid=1-s2.0-S1756464623003432-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43905614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnolia officinalis bark extract improves depressive-like behavior in DSS-induced colitis mice","authors":"Min-Soo Kim ,&nbsp;Jungim Kim ,&nbsp;Kang-In Lee ,&nbsp;Hye Jin Kim ,&nbsp;Hyungjun Kim ,&nbsp;Donghwan Kim ,&nbsp;Ki-Sun Park","doi":"10.1016/j.jff.2023.105775","DOIUrl":"10.1016/j.jff.2023.105775","url":null,"abstract":"<div><p>Most studies on the pathogenesis of inflammatory bowel disease (IBD) focus on gut function damage and gastrointestinal symptoms; however, psychiatric disorders, including depression and anxiety, have also been recognized as significant causes. Recently, we reported that <em>Magnolia officinalis</em> bark extract (MBE) alleviated enterocyte death in dextran sulfate sodium (DSS)-induced colitis mouse model that mimics IBD; however, the therapeutic effects of MBE on neurological symptoms and underlying mechanisms of IBD remain unclear. In this study, we aimed to elucidate the mechanisms by which microglia and astrocytes improve MBE-induced depression and anxiety. We found that MBE treatment improved DSS-induced weight loss, colon-length shortening, and cell death, with ameliorateon of depression/anxiety-like behavioral disorders. Systemic treatment with MBE significantly decreased DSS-induced proliferation of microglia and astrocytes in the hippocampal region, and attenuated the increase in pro-inflammatory cytokines, including IL-6 and COX-2. Interestingly, MBE alleviated DSS-induced alterations of the serotonergic system by improving serotonin levels, and expression of serotonin 4 receptor (5-HT4R) and cAMP response element-binding protein (CREB) phosphorylation in the hippocampus. Overall, our findings indicate that MBE exerts protective effects not only on gastrointestinal function but also on neuropsychiatric disorders caused by colitis, which may be developed to treat IBD.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105775"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003754/pdfft?md5=84e5ed3ad9762580b3c5fbfcf29c5968&pid=1-s2.0-S1756464623003754-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45298486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osthole alleviates food allergy by blocking IL-33/ST2 pathway and targeting ILC2-CD4+T response","authors":"Lei Wang ,&nbsp;Yu Tong ,&nbsp;Rongying Zheng,&nbsp;Chen Wang,&nbsp;Jilong Shuai,&nbsp;Jingjing Song,&nbsp;Cuiye Weng,&nbsp;Junwen Fan,&nbsp;Chuqiao Lai,&nbsp;Xiaoxiao Jia ,&nbsp;Weixi Zhang","doi":"10.1016/j.jff.2023.105737","DOIUrl":"10.1016/j.jff.2023.105737","url":null,"abstract":"<div><p>Osthole is widely used in medicine because of its anti-oxidative, anti-allergenic and anti-inflammatory. In this study, we explored the protective effects of osthole on food allergenic mice and investigated its potential mechanism of action. We first established a food allergic mouse model by intraperitoneal sensitization and OVA (ovalbumin) oral challenge. Osthole were orally administered before OVA challenge. Diarrhea and allergy scores were used to assess food allergy symptoms. The pathological changes of the intestine were examined by H&amp;E staining and electron microscopy. OVA-IgE, mucosal mast cell protease-1 (mMCP-1), IL-33, ST2, group 2 innate lymphoid cell (ILC2), and regulatory T (Treg) related-cytokine levels were assessed.We found that the oral administration of osthole after OVA sensitization could improve food allergy symptoms and induce beneficial intestinal pathological changes in mice. Osthole reduce the levels of IgE and mMCP-1 and inhibited the IL33/ST2 signaling pathway, thereby blocking ILC2 response and improving Treg response.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105737"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003377/pdfft?md5=560eccbed96a966eb5a75a65a1390e30&pid=1-s2.0-S1756464623003377-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41895263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pomegranate-derived exosome-like nanovesicles ameliorate high-fat diet-induced nonalcoholic fatty liver disease via alleviating mitochondrial dysfunction","authors":"Zuoxu Hou ,&nbsp;Xiao Wang ,&nbsp;Zefeng Yang ,&nbsp;Zhiwei Deng ,&nbsp;Jin Zhang ,&nbsp;Jintao Zhong ,&nbsp;Shanrui Liu ,&nbsp;Yuanyuan Hu ,&nbsp;Hongxun Sang","doi":"10.1016/j.jff.2023.105734","DOIUrl":"10.1016/j.jff.2023.105734","url":null,"abstract":"<div><p>Plant-derived exosome-like nanovesicles carry a variety of bioactive substances and have multiple health benefits. Pomegranate (<em>Punica granatum</em>) is a popular fruit with hepatoprotective effects. However, the effects of pomegranate-derived exosome-like nanovesicles (PENs) on nonalcoholic fatty liver disease (NAFLD) are still unknown. Here, PENs were successfully isolated and characterized, and found to be internalized by HepG2 cells. Interestingly, PENs survived gastrointestinal environment and ameliorated lipid accumulation as well as mitochondrial dysfunction in palmitic acid (PA)-treated HepG2 cells. Importantly, oral administration of PENs accumulated in the liver of mice and effectively alleviated high-fat diet (HFD)-induced NAFLD by improving mitochondrial function, as evidenced by improvements of ATP content, mitochondrial complex I activity and oxidative stress. Moreover, PENs restored SIRT3/SOD2 signaling and improved SOD2 activity in the liver of HFD-fed mice. These findings provide new mechanistic insights into pomegranate’s hepatoprotection and demonstrate the therapeutic potential of PENs for NAFLD.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105734"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003341/pdfft?md5=a7a4bc7cbc5b81660beee2a3c7953fc8&pid=1-s2.0-S1756464623003341-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42647469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective peptides isolated from flavourzyme-pea protein hydrolysate protect human SH-SY5Y cells from Aβ1-42 induced apoptosis","authors":"Lu-Sheng Hsieh ,&nbsp;Yun-Chi Hsu ,&nbsp;Wen-Dee Chiang","doi":"10.1016/j.jff.2023.105755","DOIUrl":"10.1016/j.jff.2023.105755","url":null,"abstract":"<div><p>Neuroprotective peptides derived from Flavourzyme®-pea protein hydrolysate (FPPH) were sequentially purified by chromatographies. Cell viability of β-amyloid peptide-induced oxidative damage in human neuroblastoma SH-SY5Y cells was used to explore neuroprotective effects of FPPH. Three novel neuroprotective peptides were obtained by liquid chromatography tandem mass spectrometry. <em>In vitro</em> effect of gastrointestinal proteases on neuroprotective effects of the three peptides was also investigated. The result suggested that the gastrointestinal proteases did not affect neuroprotective effects of the three novel peptides, which reveals the potential to ameliorate diseases caused by neurodegeneration. Pretreating SH-SY5Y cells with peptide GGPFKSPF (GF) before Aβ_1-42 treatment increased cell viability and reduced formation of reactive oxygen species. Decrease of mitochondrial membrane potential and anti-apoptotic proteins Bcl-2 induced by Aβ_1-42 can be restored by GF treatment. GF can remarkably decrease the contents of pro-apoptotic proteins, Bax and Caspase-3, inducing the activation of Akt/GSK-3β pathway by phosphorylation of Akt. GF exerted an antioxidant effect via Nrf2/HO-1 signaling pathway by activating Nrf2, reducing malondialdehyde level as well as increasing the expression of HO-1 and the activities of superoxide dismutase, catalase and glutathione peroxidase. As a results, GF had preventively potential as functional foods in Aβ–related neurodegenerative disease.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105755"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003559/pdfft?md5=0a895e6d445efd13415543898950b6c0&pid=1-s2.0-S1756464623003559-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45993021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The non-conventional edible plant foroba (Parkia biglobosa) has anti-obesity effect, improves lipid peroxidation and reverses colon and hippocampal lesions in healthy and obese rats","authors":"Mirela Gouveia-Nhanca ,&nbsp;Maria Luiza Rolim Bezerra ,&nbsp;Kamila Sabino Batista ,&nbsp;Rafael Oliveira Pinheiro ,&nbsp;Naís Lira Soares ,&nbsp;Maria Carolina de Paiva Sousa ,&nbsp;Adriano Francisco Alves ,&nbsp;Mateus Duarte Ribeiro ,&nbsp;Alexandre Sergio Silva ,&nbsp;Marciane Magnani ,&nbsp;Marcos dos Santos Lima ,&nbsp;Jailane de Souza Aquino","doi":"10.1016/j.jff.2023.105745","DOIUrl":"10.1016/j.jff.2023.105745","url":null,"abstract":"<div><p>We evaluated the <em>Parkia biglobosa</em> pulp (PB) effects on food intake, body composition and oxidative and inflammatory parameters related to gut and brain of rats. A total of 32 male Wistar rats were randomised into four groups: healthy (HG), obese (OG); and healthy (HPB) and obese (OPB) groups with PB (400 mg/kg body weight). The rats consumed a control or cafeteria diet for 16 weeks. Body weight, food intake, bacteria count, organic acids in faeces were quantified. NF-κB and oxidative parameters were evaluated in the colon and hippocampus. PB contains fibres, 3,4-dihydroxybenzoic acid, catechin, and myricetin. PB treatment decreased fat mass and energy intake; increased the count of lactic acid bacteria and faecal organic acids, improved lipid peroxidation and reversed colon and hippocampal lesions, with reduction the NF-κB in OPB colon. PB has anti-obesity effect with potential to modulation gut-brain axis, mainly via blocking the activation of NF-κB.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105745"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1756464623003456/pdfft?md5=143f431d6733d16738d7aa8277620a3d&pid=1-s2.0-S1756464623003456-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43265519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenolic compounds from Idesia polycarpa Maxim. fruits ameliorate non-alcoholic fatty liver disease by modulating lipid metabolism in oleic acid-induced HepG2 cells and high-fat diet-induced mice","authors":"Lei Huang ,&nbsp;Yanrong Zeng ,&nbsp;Fei Li ,&nbsp;Xiuyan Zheng ,&nbsp;Qing Rao ,&nbsp;Babu Gajendran ,&nbsp;Krishnapriya M. Varier ,&nbsp;Tong Peng ,&nbsp;Lin Tang","doi":"10.1016/j.jff.2023.105715","DOIUrl":"https://doi.org/10.1016/j.jff.2023.105715","url":null,"abstract":"<div><p>Previous reports have suggested that <em>Idesia polycarpa</em> Maxim. (<em>I. polycarpa</em>) fruits possess properties for the prevention and treatment of non-alcoholic fatty liver disease (NAFLD), owing to its lipid-lowering and anti-oxidant activities. In this study, ethyl acetate extract (EAE) of <em>I. polycarpa</em> fruits supplementation reduced body weight gain and inflammatory cytokines secretion, improved glucose tolerance and insulin sensitivity, and alleviated hepatic steatosis in high-fat diet (HFD)-fed mice. Of note, a total of eight polyphenolic compounds were isolated and identified from EAE, among which 1-[(6′-<em>O</em>-(<em>Z</em>)-<em>p</em>-coumar-oyl)-<em>β</em>-D-glucopyranosyl]-oxy-2-phenol (compound <strong>2</strong>) exhibited better lipid-lowering activity in oleic acid (OA)-induced HepG2 cells than other compounds. Moreover, using hepatic steatosis cell model and PPAR-luciferase reporter system, we found that compound <strong>2</strong> reduced lipid accumulation and lipotoxicity, and inhibited inflammatory reaction via the PPAR-α-mediated improvement of lipid metabolism. This experiment will provide a theoretical basis for intervention with <em>I. polycarpa</em> fruits in delaying the occurrence and development of NAFLD.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105715"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3034966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conversion of Baijiu distillers’ grains to functional peptides: Process optimization and antioxidant activity evaluation","authors":"Xiaogang Liu ,&nbsp;Rui Chang ,&nbsp;Zhilei Zhou ,&nbsp;Qingxi Ren ,&nbsp;Caihong Sheng ,&nbsp;Yu Lan ,&nbsp;Xiaonian Cao ,&nbsp;Jian Mao","doi":"10.1016/j.jff.2023.105722","DOIUrl":"https://doi.org/10.1016/j.jff.2023.105722","url":null,"abstract":"<div><p>Distiller’s grains resourcing is a crucial problem for the sustainable development of the Chinese <em>Baijiu</em> industry. In order to improve the protein utilization of distiller’s grains. In this study, distiller’s grains pretreated by wet crushing combined with amylase/cellulase were further hydrolyzed with neutral protease combined with papain at 50 °C for 3 h. The hydrolysates were decolorized by 1% coconut shell activated carbon for 15 min, and separated and purified by macroporous resin and Sephadex gel to yield peptide fractions (F1, F2, F3 and F4). The F3 fraction with the highest radical scavenging activity was analyzed by nano-LC-MS/MS and peptidomics to identify peptides. Three peptides (especially newly discovered AAHVLAAAFL) displayed strong alcohol solubility and antioxidant activity and were stably bound to key regulatory pathway receptors (Keap1-Nrf2) and myeloperoxidase (MPO), further elucidating molecular mechanism of anti-oxidation. These findings provided knowledge for potential functional peptide candidates and facilitates the high-value commercial development of distiller’s grains.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105722"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2824852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gypenosides and capsaicinoids in combination ameliorates high-fat-diet- induced rat hyperlipidemia via the PPARγ-LXRα-ABCA1/ABCG1 pathway","authors":"Fangbo Zhang ,&nbsp;Yu Li ,&nbsp;Weijuan Xin ,&nbsp;Lifang Wang ,&nbsp;Yi Zhang ,&nbsp;He Xu ,&nbsp;Hongjie Wang ,&nbsp;Haiyu Zhao ,&nbsp;Hongjun Yang ,&nbsp;Nan Si ,&nbsp;Baolin Bian","doi":"10.1016/j.jff.2023.105714","DOIUrl":"https://doi.org/10.1016/j.jff.2023.105714","url":null,"abstract":"<div><p>Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatic disease characterized by excessive fat accumulation, and is the major risk for cardiovascular and metabolic disorders. We explored the therapeutic effect of gypenosides (Gyps) in combination with capsaicinoids (Caps) against high-fat-diet (HFD)-induced rat hyperlipidemia and oxidized low-density lipoprotein (ox-LDL)-treated Raw264.7 cells. Network pharmacology analysis predicted that the potential molecular mechanism of Gyps/Caps combination against hyperlipidemia was mainly associated with steroid metabolism, lipid synthesis and inflammatory response. Animal experiment demonstrated that Gyps/Caps combination lowered the liver weight, liver index, serum lipid profile and hepatic cholesterol content, improved liver function by reducing the serum markers of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), thereby relieving the hepatic injury. Moreover, Gyps/Caps combination inhibited oxidative stress by decreasing the lactic dehydrogenase (LDH) activity and increasing the superoxide dismutase (SOD) activity. Western blotting result suggested that Gyps/Caps combination could upregulate the expressions of peroxisome proliferator-activated receptors γ (PPARγ), liver X receptor α (LXRα), ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) in the liver tissue. Cellular experiment revealed that the combination treatment protected RAW264.7 cells from ox-LDL induction through inhibiting apoptosis, lipid-decreasing and anti-inflammation, and caused the dramatic enhancement on PPARγ expression. Notably, the combined use of Gyps and Caps did not influence the pharmacological outcomes of each other, but significantly enhanced the overall therapeutic effect in ameliorating HFD-induced hyperlipidemia. Our research provided an experimental basis for advanced research and clinical application of Gyps/Caps combination. Importantly, our cumulative data indicated that combination use of Gyps and Caps may be an effective therapeutic strategy for alleviating hyperlipidemia-induced NAFLD.</p></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"108 ","pages":"Article 105714"},"PeriodicalIF":5.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2887788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}