Journal of Food and Drug Analysis最新文献

{"title":"Simultaneous determination of 24 congeners of 2- and 3-monochloropropanediol esters and 7 congeners of glycidyl esters using direct multi-residue analytical LC-MS/MS methods in various food matrices.","authors":"Ching-Chang Lee,&nbsp;Bo-Lun Lin,&nbsp;Yi-Wen Huang,&nbsp;Ning-Syuan Hsu,&nbsp;Wei-Hsiang Chang","doi":"10.38212/2224-6614.3442","DOIUrl":"https://doi.org/10.38212/2224-6614.3442","url":null,"abstract":"<p><p>Glycidyl esters (GEs) and 2- and 3-monochloropropanediol esters (MCPDEs) are emerging process-generated food contaminants known as possible carcinogens. Herein, a direct method is developed and validated for the first time to simultaneously quantify seven GEs and twenty-four MCPDE congeners of processed foods using liquid chromatography-tandem mass spectrometry in a single sequence without ester cleavage or derivatisation, thereby allowing for the simultaneous analysis of numerous food matrices with high accuracy and precision. Our results show levels of GEs varying from <LOQ to 13486 ng/g, whereas those of MCPDEs range from <LOQ to 12019 ng/g, respectively.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"31 1","pages":"55-72"},"PeriodicalIF":3.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/a5/jfda-31-01-055.PMC10208668.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guilu Erxian Jiao enhances protein synthesis, glucose homeostasis, mitochondrial biogenesis and slow-twitch fibers in the skeletal muscle.","authors":"Wei-Yu Fang,&nbsp;Wan-Hsuan Chang,&nbsp;Yi-Hong Tsai,&nbsp;Hung-Te Hsu,&nbsp;Fang-Rong Chang,&nbsp;Chih-Lung Lin,&nbsp;Yi-Ching Lo","doi":"10.38212/2224-6614.3435","DOIUrl":"https://doi.org/10.38212/2224-6614.3435","url":null,"abstract":"<p><p>Guilu Erxian Jiao (GEJ) is a commonly used nutritional supplement due to its rich content of amino acids. It is also a traditional herbal medicine for improving degenerative joint. This study aimed to investigate the effect and mechanism of GEJ water extract (GEJ-WE) on skeletal muscle in C2C12 myotubes and C57BL/6J mice. Analysis of GEJ-WE were performed by high-performance liquid chromatography fingerprinting with chemical standards. Protein expression, mRNA level, glycogen content, mitochondria activity and ATP level were evaluated by western blots, real-time PCR, PAS staining, MTT and ATP bioluminescence assay, respectively. Skeletal muscle strength was evaluated by grip strength. Skeletal muscle volume, mass and fiber types were evaluated by micro computed tomography, histological analysis and immunofluorescence staining, respectively. Motor function was evaluated by rotarod performance and locomotor activity. In C2C12 myotubes, GEJ-WE significantly enhanced myogenic differentiation and myotube growth, protein synthesis signaling IGF-1/IGF-1R/IRS-1/Akt, Glut4 translocation, glycogen content, mitochondrial biogenesis signaling PGC-1α/NRF1/TFAM, mitochondrial activity and ATP production. However, IGF-1R antagonist AG1024 and PI3K inhibitor wortmannin reduced GEJ-WE-induced protein expression of MyHC, p-Akt, p-mTOR and p-GSK-3β, Glut4 translocation and glycogen content. In C57BL/6J mice, GEJ-WE not only upregulated protein synthesis and mitochondrial biogenesis signaling, but it also increased muscle volume, relative muscle weight, cross-sectional area of myofibers, glycogen content and transition of fast-to-slow type fibers of skeletal muscles. Moreover, GEJ-WE enhanced grip strength and motor activity of mice. In conclusion, the upregulation of protein synthesis, myogenic differentiation, glucose homeostasis, mitochondrial biogenesis and slow-twitch fibers contributes to the mechanisms of GEJ-WE on the enhancement of skeletal muscle mass and motor function.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"31 1","pages":"116-136"},"PeriodicalIF":3.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/aa/jfda-31-01-116.PMC10208666.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics analysis assisted by signal selection for comprehensively identifying metabolites of new psychoactive substances: 4-MeO-α-PVP as an example.","authors":"Hsin-Yi Wu, Yuan-Chih Chen, Jing-Fang Hsu, Hsiang-Ting Lu, Yu-Yi Pan, Mi-Chia Ma, Pao-Chi Liao","doi":"10.38212/2224-6614.3447","DOIUrl":"10.38212/2224-6614.3447","url":null,"abstract":"<p><p>New psychoactive substances (NPS) have been rapidly emerged as legal alternatives to controlled drugs, which raised severe public health issue. The detection and monitoring of its intake by complete metabolic profiling is an urgent and vital task. Untargeted metabolomics approach has been applied for several NPS metabolites studies. Although the number of such works is relatively limited but with a rapidly growing need. The present study aimed to propose a procedure that includes liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis and a signal selection software, MetaboFinder, programed as a web tool. The comprehensive metabolites profile of one kind of NPS, 4-methoxy-α-pyrrolidinovalerophenone (4-MeO-α-PVP), was studied by using this workflow. In this study, two different concentrations of 4-MeO-α-PVP along with as blank sample were incubated with human liver S9 fraction for the conversion to their metabolites and followed by LC-MS analysis. After retention time alignment and feature identification, 4640 features were obtained and submitted to statistical analysis for signal selection by using MetaboFinder. A total of 50 features were considered as 4-MeO-α-PVP metabolite candidates showing significant changes (p < 0.00001 and fold change >2) between the two investigated groups. Targeted LC-MS/MS analysis was conducted focusing on these significantly expressed features. Assisted by chemical formula determination according to high mass accuracy and in silico MS² fragmentation prediction, 19 chemical structure identifications were achieved. Among which, 8 metabolites have been reported derived from 4-MeO-α-PVP in a previous literature while 11 novel 4-MeO-α-PVP metabolites were identified by using our strategy. Further in vivo animal experiment confirmed that 18 compounds were 4-MeO-α-PVP metabolites, which demonstrated the feasibility of our strategy for screening the 4-MeO-α-PVP metabolites. We anticipate that this procedure may support and facilitate traditional metabolism studies and potentially being applied for routine NPS metabolites screening.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"31 1","pages":"137-151"},"PeriodicalIF":2.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ae/ca/jfda-31-01-137.PMC10208664.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution ¹H NMR analysis of continuous and discontinuous thermo-oxidative susceptibility of culinary oils during frying at 180 °C.","authors":"Gilbert Ampem, Adam Le Gresley, Martin Grootveld, Declan P Naughton","doi":"10.38212/2224-6614.3439","DOIUrl":"10.38212/2224-6614.3439","url":null,"abstract":"<p><p>Lipid oxidations products (LOPs) are reactive mutagenic and carcinogenic species known to be generated in thermally stressed culinary oils. Mapping the evolution of LOPs in culinary oils exposed to standard frying practices - both continuous and discontinuous thermo-oxidation - at 180 °C is vital to our understanding of these processes, and to the development of scientific solutions for their effective suppression. Modifications in the chemical compositions of the thermo-oxidised oils were analysed using a high-resolution proton nuclear magnetic resonance (¹H NMR) technique. Research findings acquired showed that polyunsaturated fatty acid (PUFA)-rich culinary oils were the most susceptible to thermo-oxidation. Consistently, coconut oil, which has a very high saturated fatty acid (SFA) content, was highly resistant to the thermo-oxidative methods employed. Furthermore, continuous thermo-oxidation produced greater substantive changes in the oils evaluated than discontinuous episodes. Indeed, for 120 min thermo-oxidation durations, both continuous and discontinuous methods exerted a unique impact on the contents and levels of aldehydic LOPs formed in the oils. This report exposes daily used culinary oils to thermo-oxidation, and therefore, it permits assessments of their peroxidative susceptibilities. It also serves as a reminder to the scientific community to investigate approaches for suppressing toxic LOPs generation in culinary oils exposed to these processes, most notably those involving their reuse.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"31 1","pages":"95-115"},"PeriodicalIF":2.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b3/20/jfda-31-01-095.PMC10208671.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9993490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a metabolomics-based data analysis approach for identifying drug metabolites based on high-resolution mass spectrometry.","authors":"Hsiao-Hsien Ting,&nbsp;Yi-Shiou Chiou,&nbsp;Tien-Yi Chang,&nbsp;Guan-Yu Lin,&nbsp;Pei-Jhen Li,&nbsp;Chia-Lung Shih","doi":"10.38212/2224-6614.3451","DOIUrl":"https://doi.org/10.38212/2224-6614.3451","url":null,"abstract":"A metabolomics-based approach to data analysis is required for drug metabolites to be identified quickly. This study developed such an approach based on high-resolution mass spectrometry. Our approach is a two-stage one that combines a time-course experiment with stable isotope tracing. Pioglitazone (PIO) was used to improve glycemic management for type 2 diabetes mellitus. Consequently, PIO was taken as a model drug for identifying metabolites. During Stage I of data analysis, 704 out of 26626 ions exhibited a positive relationship between ion abundance ratio and incubation time in a time-course experiment. During Stage II, 25 isotope pairs were identified among the 704 ions. Among these 25 ions, 18 exhibited a dose-response relationship. Finally, 14 of the 18 ions were verified to be PIO structure-related metabolite ions. Otherwise, orthogonal partial least squares-discriminant analysis (OPLS-DA) was adopted to mine PIO metabolite ions, and 10 PIO structure-related metabolite ions were identified. However, only four ions were identified by both our developed approach and OPLS-DA, indicating that differences in the designs of metabolomics-based approaches to data analysis can result in differences in which metabolites are identified. A total of 20 PIO structure-related metabolites were identified by our developed approach and OPLS-DA, and six metabolites were novel. The results demonstrated that our developed two-stage data analysis approach can be used to effectively mine data on PIO metabolite ions from a relatively complex matrix.","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"31 1","pages":"152-164"},"PeriodicalIF":3.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8a/e5/jfda-31-01-152.PMC10208669.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple and rapid detection of ractopamine in pork with comparison of LSPR and LFIA sensors.","authors":"Min Ji Lee,&nbsp;Sheik Aliya,&nbsp;Eun-Seon Lee,&nbsp;Tae-Joon Jeon,&nbsp;Mi-Hwa Oh,&nbsp;Yun Suk Huh","doi":"10.38212/2224-6614.3410","DOIUrl":"https://doi.org/10.38212/2224-6614.3410","url":null,"abstract":"<p><p>This study developed a simple and rapid strategic technique to detect ractopamine (chemical growth-promoting agent) in pork. Two highly sensitive and specific gold nanoparticle-based portable sensors, i.e., localized surface plasmon resonance (LSPR) sensors, and lateral flow immunoassay (LFIA) strips were developed to detect veterinary drug residues in food products, that have detrimental effects on humans. Optimization studies were conducted on several sensor devices to improve sensitivity. Each sensor comprised functionalized gold nanoparticles conjugated with ractopamine antibodies. The LSPR sensor chip achieved excellent detection sensitivity = 1.19 fg/mL and was advantageous for quantitative analysis due to its wide dynamic range. On the other hand, LFIA strips provided visual test confirmation and achieved 2.27 ng/mL detection sensitivity, significantly less sensitive than LSPR. The complementary sensors help overcome each other's shortcomings with both the techniques offering ease of use, affordability and rapid diagnosis. Thus, these sensors can be applied on-site for routine screening of harmful drug residues in pork meat. They also provide useful direction for advanced technologies to enhance assay performance for detecting various other food drug contaminants.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"30 4","pages":"590-602"},"PeriodicalIF":3.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/03/jfda-30-04-590.PMC9910298.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10743029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface.","authors":"Bing-Huei Chen","doi":"10.38212/2224-6614.3443","DOIUrl":"https://doi.org/10.38212/2224-6614.3443","url":null,"abstract":"","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"30 4","pages":"i"},"PeriodicalIF":3.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/c9/jfda-30-04-i.PMC9910301.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment of Reviewers.","authors":"","doi":"10.38212/2224-6614.3444","DOIUrl":"https://doi.org/10.38212/2224-6614.3444","url":null,"abstract":"","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"30 4","pages":"ii"},"PeriodicalIF":3.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9237896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment of Reviewers","authors":"Hideo Adachi, Tomohiko Adachi, Shinichi Aishima, Koichi Aiura, Yoshito Akagi, T. Akahoshi, Shinji Akamine, Sadako Akashi, Kohei Akazawa, Sadanori Akita, Seiji Akiyama, Yasunori Akutsu, M. Alifano, Atsushi Amano, Sadao Amano, L. Ampollini, Hisami Ando, Hideaki Andoh, M. Anraku, K. Aogi, T. Aoki, Tatsuya Aoki, Yasumi Araki, T. Asai, Yuuko Asano, T. Asao, Y. Asato, Katsuhiro Asonuma, Nobuyoshi Azuma, Hideo Baba, Y. Baba, Emre Balik, H. Bando, T. Bando, T. Beppu","doi":"10.1007/s00595-011-0059-7","DOIUrl":"https://doi.org/10.1007/s00595-011-0059-7","url":null,"abstract":"","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"30 1","pages":"ii - ii"},"PeriodicalIF":3.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00595-011-0059-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43021877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the analysis of polycyclic aromatic hydrocarbons in food and water.","authors":"Bing-Huei Chen,&nbsp;Baskaran Stephen Inbaraj,&nbsp;Kuo-Chiang Hsu","doi":"10.38212/2224-6614.3429","DOIUrl":"https://doi.org/10.38212/2224-6614.3429","url":null,"abstract":"<p><p>Polycyclic aromatic hydrocarbons (PAHs), a class of harmful and persistent organic contaminant, are widely distributed in the environment and eventually accumulated in water and food. Also, they are formed in different varieties and varying amounts during processing of food depending on the food composition, cooking method and processing condition. According to the International Agency for Research on Cancer (IARC), various PAHs are classified under Group 1 to 3 category, with Group 1 designated as carcinogenic to humans, Group 2A as probable carcinogen, Group 2B as possible carcinogen and Group 3 as noncarcinogenic. Therefore, it is imperative to develop rapid and highly sensitive analytical methods for determination of PAHs in food and water. This article aims to overview the recent advances of various chromatographic methods as well as electrochemical and SERS-based optical sensing methods for analysis of PAHs in food and water. Initially, several conventional sample preparation methods along with the advanced extraction for isolation of PAHs were summarized, followed by reviewing various gas chromatographic methods coupled with various detection techniques for PAHs analysis in various food products including meat/meat products, seafood, oil, milk/milk products, baby foods, honey, vegetable, cocoa products, tea/coffee, juice, rice, flour, noodle and cake. In addition, high performance liquid chromatographic methods coupled with fluorescence, diode array or mass/tandem mass detection techniques as well as an emerging supercritical fluid chromatographic technique employed for determination of PAHs in different food and water matrices were also overviewed. Finally, various electrochemical sensors and SERS-based optical sensors developed recently for onsite detection of PAHs were tabulated and discussed. Thus, this review article can provide a research update on chromatography and sensor-based analytical methods for PAH analysis as well as enable elucidation of research gaps for future studies.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"30 4","pages":"494-522"},"PeriodicalIF":3.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/48/jfda-30-04-494.PMC9910297.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10733946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}