Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology最新文献_第9页

B chromosomes of B. dichromosomatica show a reduced level of euchromatic histone H3 methylation marks. 二色体B染色体显示出低水平的常染色组蛋白H3甲基化标记。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-02-05 DOI: 10.1007/s10577-006-1114-x

Sylvia Marschner, Katrin Kumke, Andreas Houben

{"title":"B chromosomes of B. dichromosomatica show a reduced level of euchromatic histone H3 methylation marks.","authors":"Sylvia Marschner, Katrin Kumke, Andreas Houben","doi":"10.1007/s10577-006-1114-x","DOIUrl":"https://doi.org/10.1007/s10577-006-1114-x","url":null,"abstract":"B chromosomes (Bs) are dispensable, less-transcriptionally active components of the genomes of numerous species. Little information is available on the chromatin composition of Bs and whether it differs in any way from that of the A chromosomes. Methylated isoforms of histone H3 are of particular interest because of their role in eu/heterochromatin formation. Immunofluorescence using site-specific antibodies demonstrates that the chromatin in A and both types of Bs of B. dichromosomatica differs markedly in euchromatic histone H3 methylation marks. While A chromosomes are labelled brightly, the micro B and large B chromosomes are faintly labelled with antibodies against H3K4me2/3, H3K9me3 and H3K27me2/3. The heteropycnotic, tandem-repeat enriched micro Bs were even less labelled with euchromatic histone H3 methylation marks than large Bs, most probably due to different DNA composition. No differences in immunolabelling intensity between A and B chromosomes were found as to the heterochromatic marks H3K9me1/2 and H3K27me1, indicating that Bs are not additionally labelled by heterochromatin typical histone H3 modifications. Analysis of DNA replication timing suggests that micro Bs are replicating throughout S-phase.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"215-22"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-006-1114-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26595616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Lamins A and C are present in the nuclei of early porcine embryos, with lamin A being distributed in large intranuclear foci. 层粘连蛋白A和层粘连蛋白C存在于早期猪胚胎的细胞核中，层粘连蛋白A分布在较大的核内灶。

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-01-09 DOI: 10.1007/s10577-006-1088-8

Helen A Foster, Paula Stokes, Katherine Forsey, Henry J Leese, Joanna M Bridger

{"title":"Lamins A and C are present in the nuclei of early porcine embryos, with lamin A being distributed in large intranuclear foci.","authors":"Helen A Foster, Paula Stokes, Katherine Forsey, Henry J Leese, Joanna M Bridger","doi":"10.1007/s10577-006-1088-8","DOIUrl":"10.1007/s10577-006-1088-8","url":null,"abstract":"Gametogenesis and embryogenesis are dynamic developmental stages marked by extensive modifications in the organization of the genome and nuclear architecture. In the literature it is conveyed that only B-type lamins are required in these early stages of development and that A-type lamins are not present or required until differentiation of specific cell types associated with specialized tissue is initiated. To assess the presence of nuclear structures that are putatively involved in genome regulation, we investigated the distribution of lamin proteins throughout the early stages of porcine embryonic development, using testes tissue sections, oocytes and in-vitro fertilized (IVF) porcine embryos and employing anti-lamin antibodies. We have shown that anti-lamin A staining is present at the one-cell, two-cell, four-cell, and six- to eight-cell stages of early porcine embryo development, but diminishes at the morulae and blastocyst stages. Large intranuclear anti-lamin A foci are prominent in the early preimplantation stages. Both anti-lamin A/C and anti-lamin B staining were clearly present in all embryonic stages. Immature porcine oocytes revealed lamin rings using the monoclonal anti-lamin A/C antibody and many immature oocytes exhibited a pale rim staining pattern with anti-lamin A antibody. A-type lamins were not observed in sperm precursor cells. Thus, we have shown that A-type lamins and B-type lamins are present at the nuclear envelope in very early porcine embryos and that lamin A is also found in large intranuclear aggregates in two-cell to eight-cell embryos but is lacking from later embryonic stages.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"163-74"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26472716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of colchicine and telocentric chromosome conformation on centromere and telomere dynamics at meiotic prophase I in wheat-rye additions. 秋水仙碱和端粒染色体构象对小麦-黑麦减数分裂前期着丝粒和端粒动力学的影响。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-02-20 DOI: 10.1007/s10577-006-1117-7

Eduardo Corredor, Tomás Naranjo

{"title":"Effect of colchicine and telocentric chromosome conformation on centromere and telomere dynamics at meiotic prophase I in wheat-rye additions.","authors":"Eduardo Corredor, Tomás Naranjo","doi":"10.1007/s10577-006-1117-7","DOIUrl":"https://doi.org/10.1007/s10577-006-1117-7","url":null,"abstract":"Association of telomeres in a bouquet and clustering of centromere regions have been proposed to be involved in the search and recognition of homologous partners. We have analysed the role of these structures in meiotic chromosome pairing in wheat-rye addition lines by applying colchicine for disturbing presynaptic telomere movements and by modifying the centromere position from submetacentric to telocentric for studying centromere effects. Rye chromosomes, wheat and rye centromeres, and telomeres were identified by fluorescence in-situ hybridization. Presynaptic association of centromeres in pairs or in more complex structures involved mainly non-homologous chromosomes as deduced from the behaviour of rye centromeres. While centromere association was not affected by colchicine, colchicine inhibited bouquet formation, which caused failure of homologous synapsis. Homologous centromeres of rye telocentrics associated earlier than those of rye submetacentric chromosomes, indicating that migration of the telocentrics' centromeres to the telomere pole during bouquet formation facilitated their association. Homologous chromosomes associated in premeiotic interphase can recognize each other and initiate synapsis at zygotene. However, telomere convergence is needed for bringing together the majority of homologous pairs that normally occupy separate territories in premeiotic nuclei.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"231-45"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-006-1117-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26560361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Characterisation of Zygosaccharomyces rouxii centromeres and construction of first Z. rouxii centromeric vectors. 柔丝酵母着丝粒的鉴定及首个柔丝酵母着丝粒载体的构建。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-05-15 DOI: 10.1007/s10577-007-1136-z

Lenka Pribylova, Marie-Laure Straub, Hana Sychrova, Jacky de Montigny

引用次数: 15

Establishment of high-resolution FISH mapping system and its application for molecular cytogenetic characterization of chromosomes in newt, Cynops pyrrhogaster (Urodela, Amphibia). 高分辨率FISH定位系统的建立及其在蝾螈(尾纲，两栖纲)染色体分子细胞遗传学鉴定中的应用

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-05-30 DOI: 10.1007/s10577-007-1139-9

Takahiro Murakami, Nobuyasu Maki, Chizuko Nishida-Umehara, Yoichi Matsuda, Kiyokazu Agata

{"title":"Establishment of high-resolution FISH mapping system and its application for molecular cytogenetic characterization of chromosomes in newt, Cynops pyrrhogaster (Urodela, Amphibia).","authors":"Takahiro Murakami, Nobuyasu Maki, Chizuko Nishida-Umehara, Yoichi Matsuda, Kiyokazu Agata","doi":"10.1007/s10577-007-1139-9","DOIUrl":"https://doi.org/10.1007/s10577-007-1139-9","url":null,"abstract":"Urodele amphibians (newts and salamanders) are important animal models for understanding regeneration mechanisms and genome evolution. We constructed ideograms of BrdU/dT- and C-banded karyotypes in the Japanese fire-belly newt, Cynops pyrrhogaster, which is useful as a model animal with extremely high ability of regeneration. We also established a high-resolution FISH mapping system for newts, and localized satellite DNA sequences, 18S rDNAs, telomeric (TTAGGG)n repeats and seven functional genes, including genes associated with lens regeneration, tyrosinase and two types of gamma crystallins, to chromosomes of the newt. The 18S rDNAs were localized to three chromosomal pairs in males, whereas the chromosomal locations were highly variable in females. No hybridization signals were detected for the telomeric (TTAGGG)n sequence. All three lens regeneration-related genes were mapped on the short arm of chromosome 7, suggesting that the location of the genes in the same linkage group may be correlated with the regulation of gene expression associated with chromatin dynamics in interphase nuclei during regeneration. The chromosomal distribution and nucleotide sequences of pericentric satellite DNA sequences were well conserved between C. pyrrhogaster and European newts; in contrast, there was species specificity of nucleotide sequences for centromere-specific satellite DNAs.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"471-84"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1139-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26743405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Protein immunolocalization supports the presence of identical mechanisms of XY body formation in eutherians and marsupials. 蛋白质免疫定位支持在真动物和有袋动物中存在相同的XY体形成机制。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-09-13 DOI: 10.1007/s10577-007-1165-7

M J Franco, R B Sciurano, A J Solari

{"title":"Protein immunolocalization supports the presence of identical mechanisms of XY body formation in eutherians and marsupials.","authors":"M J Franco, R B Sciurano, A J Solari","doi":"10.1007/s10577-007-1165-7","DOIUrl":"https://doi.org/10.1007/s10577-007-1165-7","url":null,"abstract":"The meiotic sex chromosomes of the American marsupials Monodelphis dimidiata and Didelphis albiventris were studied with electron microscopy (EM) and with immunofluorescence localization of meiotic proteins SYCP1 and SYCP3, and proteins essential for meiotic sex chromosome inactivation (MSCI), gamma-H2AX and BRCA1. The chromatin of the non-synaptic X and Y chromosomes contains gamma-H2AX, first as foci and then as homogeneous staining at late stages. The thick and split X and Y axes are labelled with BRCA1 except at one terminus. The bulgings of the axes contain SYCP1 as well as the inner side of the dense plate. The evenly spaced and highly packed chromatin fibres of the conjoined XY body in these species have the same behaviour and the same components (gamma-H2AX in the chromatin, BRCA1 in the axes) as in the XY body of eutherian species. These observations and recent data from the literature suggest that XY body formation is ancestral to the metatherian-eutherian divergence.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"815-24"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1165-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40956070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

The nonrandom distribution of long clusters of all possible classes of trinucleotide repeats in barley chromosomes. 大麦染色体中所有可能类型的三核苷酸重复序列的长簇的非随机分布。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-08-23 DOI: 10.1007/s10577-007-1156-8

Angeles Cuadrado, Nicolas Jouve

{"title":"The nonrandom distribution of long clusters of all possible classes of trinucleotide repeats in barley chromosomes.","authors":"Angeles Cuadrado, Nicolas Jouve","doi":"10.1007/s10577-007-1156-8","DOIUrl":"https://doi.org/10.1007/s10577-007-1156-8","url":null,"abstract":"This paper is the first to report the long-range organization of all possible classes of trinucleotide motifs in a higher plant genome. Fluorescent in situ hybridization (FISH), employing the synthetic oligonucleotides (AAC)5, (AAG)5, (AAT)5, (AGG)5, (CAC)5, (CAT)5, (CAG)5, (ACT)5, (ACG)5 and (GCC)5, was used to characterize the nonrandom and motif-dependent distribution of tandem arrays of trinucleotide repeats in the metaphase chromosomes and interphase nuclei of barley (Hordeum vulgare L.). This provided detailed information on the sequence content of barley chromatin and allowed the saturation of the physical map of all barley chromosomes. The following conclusions were also drawn: (1) Except for (AAT)5 and (GCC)5, the studied repetitive motifs have a characteristic pattern of distribution in terms of their in situ FISH signals. Some permit the accurate identification of individual chromosomes. (2) (CAG)5, (CAT)5 and (ACT)5 are not found in all barley chromosomes. (3) With the exception of (ACT)5, the remaining trinucleotide repeats occur predominantly in the heterochromatin and are largely absent from the euchromatic regions. Moreover, (CAC)5, (ACG)5 and (CAG)5 are exclusively concentrated in the centromeres. The employment of simple synthetic probes for the identification of chromosomes and genomic characterization, and their importance in studies on genome organization, function and evolution, are discussed.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"711-20"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1156-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40977725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera). 鳞翅目蚜蛾W染色体的分子分化。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-11-07 DOI: 10.1007/s10577-007-1173-7

Magda Vítková, Iva Fuková, Svatava Kubícková, Frantisek Marec

{"title":"Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera).","authors":"Magda Vítková, Iva Fuková, Svatava Kubícková, Frantisek Marec","doi":"10.1007/s10577-007-1173-7","DOIUrl":"https://doi.org/10.1007/s10577-007-1173-7","url":null,"abstract":"Most Lepidoptera have a WZ/ZZ sex chromosome system. We compared structure of W chromosomes in four representatives of the family Pyralidae--Ephestia kuehniella, Cadra cautella, Plodia interpunctella, and Galleria mellonella--tracing pachytene bivalents which provide much higher resolution than metaphase chromosomes. In each species, we prepared a W-chromosome painting probe from laser-microdissected W-chromatin of female polyploid nuclei. The Ephestia W-probe was cross-hybridized to chromosomes of the other pyralids to detect common parts of their W chromosomes, while the species-specific W-probes identified the respective W chromosome. This so-called Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. The results were consistent with phylogenetic relationships between the species. We also performed comparative genomic hybridization, which indicated that the W chromosome of C. cautella is composed mainly of repetitive DNA common to both sexes but accumulated in the W chromosome, whereas E. kuehniella, P. interpunctella, and G. mellonella W chromosomes also possess a large amount of female specific DNA sequences, but differently organized. Our results support the hypothesis of the accelerated molecular divergence of the lepidopteran W chromosomes in the absence of meiotic recombination.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"917-30"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1173-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41017182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA. 细粒索螨(索螨科，细粒索螨科)的极长端粒含有核糖体DNA。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-10-01 DOI: 10.1007/s10577-007-1170-x

Natalia S Zhdanova, Julia M Minina, Tatjana V Karamisheva, Irena Draskovic, Nikolai B Rubtsov, Jose-Arturo Londoño-Vallejo

{"title":"The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA.","authors":"Natalia S Zhdanova, Julia M Minina, Tatjana V Karamisheva, Irena Draskovic, Nikolai B Rubtsov, Jose-Arturo Londoño-Vallejo","doi":"10.1007/s10577-007-1170-x","DOIUrl":"https://doi.org/10.1007/s10577-007-1170-x","url":null,"abstract":"Two closely related shrew species, Sorex granarius and Sorex araneus, in which Robertsonian rearrangements have played a primary role in karyotype evolution, present very distinct telomere length patterns. S. granarius displays hyperlong telomeres specifically associated with the short arms of acrocentrics, whereas telomere lengths in S. araneus are rather short and homogenous. Using a combined approach of chromosome and fibre FISH, modified Q-FISH, 3D-FISH, Ag-NOR staining and TRF analysis, we carried out a comparative analysis of telomeric repeats and rDNA distribution on chromosome ends of Sorex granarius. Our results show that rDNA sequences forming active nuclear organizing regions are interspersed with the long telomere tracts of all short arms of acrocentrics. These observations suggest that the major rearrangements that gave rise to today's karyotype in S. granarius were accompanied by a profound reorganization of chromosome ends, which comprised extensive amplification of telomeric and rDNA repeats on the short arms of acrocentrics and finally contributed to the stabilization of telomeres. This is the first time that such telomeric structures have been observed in any mammalian species.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"881-90"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1170-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41065341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Intra- and intergenomic homology of B-genome chromosomes in trigenomic combinations of the cultivated Brassica species revealed by GISH analysis. GISH分析揭示栽培芸苔种三基因组组合中b基因组染色体的基因组内和基因组间同源性。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-10-01 DOI: 10.1007/s10577-007-1168-4

Xian-Hong Ge, Zai-Yun Li

{"title":"Intra- and intergenomic homology of B-genome chromosomes in trigenomic combinations of the cultivated Brassica species revealed by GISH analysis.","authors":"Xian-Hong Ge, Zai-Yun Li","doi":"10.1007/s10577-007-1168-4","DOIUrl":"https://doi.org/10.1007/s10577-007-1168-4","url":null,"abstract":"Intragenomic chromosome homology in the B genome of Brassica nigra and their homoeology with the chromosomes of the A-genome of B. rapa and C-genome of B. oleracea was investigated in triploids (ABC, n = 27) of different origins obtained following hybridizations between natural B. napus (AACC, 2n = 38) x B. nigra (BB, 2n = 16) [AC.B], synthetic B. napus x B. nigra [A.C.B] and B. carinata (BBCC, 2n = 34) x B. rapa (AA, 2n = 20) [BC.A]. A relatively high percentage of pollen mother cells (PMCs) with at least one B-genome chromosome paired allosyndetically with A/C chromosomes was evident in all three combinations. A maximum of three B-genome chromosomes undergoing allosyndesis per cell was observed in AC.B and A.C.B combinations. A maximum of two autosyndetic bivalents within the B genome appeared at diakinesis in all combinations. The accurate analyses of auto- and allo-syndetic pairing for B genome in trigenomic combinations provided further evidence for the hypothesis that the three basic diploid genomes of the cultivated Brassica species evolved from one common ancestral genome with a lower chromosome number. The results showed that Brassica diploids may not be ancient polyploids but may have undergone chromosomal duplications instead of whole-genome duplication. The relevance of these results along with genetic changes of progenitor genomes which occurred during the evolution of Brassica polyploids is discussed.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"849-61"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1168-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41065343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52