Magda Vítková, Iva Fuková, Svatava Kubícková, Frantisek Marec
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This so-called Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. The results were consistent with phylogenetic relationships between the species. We also performed comparative genomic hybridization, which indicated that the W chromosome of C. cautella is composed mainly of repetitive DNA common to both sexes but accumulated in the W chromosome, whereas E. kuehniella, P. interpunctella, and G. mellonella W chromosomes also possess a large amount of female specific DNA sequences, but differently organized. Our results support the hypothesis of the accelerated molecular divergence of the lepidopteran W chromosomes in the absence of meiotic recombination.</p>","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"917-30"},"PeriodicalIF":0.0000,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1173-7","citationCount":"59","resultStr":"{\"title\":\"Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera).\",\"authors\":\"Magda Vítková, Iva Fuková, Svatava Kubícková, Frantisek Marec\",\"doi\":\"10.1007/s10577-007-1173-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Most Lepidoptera have a WZ/ZZ sex chromosome system. We compared structure of W chromosomes in four representatives of the family Pyralidae--Ephestia kuehniella, Cadra cautella, Plodia interpunctella, and Galleria mellonella--tracing pachytene bivalents which provide much higher resolution than metaphase chromosomes. In each species, we prepared a W-chromosome painting probe from laser-microdissected W-chromatin of female polyploid nuclei. The Ephestia W-probe was cross-hybridized to chromosomes of the other pyralids to detect common parts of their W chromosomes, while the species-specific W-probes identified the respective W chromosome. This so-called Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. The results were consistent with phylogenetic relationships between the species. We also performed comparative genomic hybridization, which indicated that the W chromosome of C. cautella is composed mainly of repetitive DNA common to both sexes but accumulated in the W chromosome, whereas E. kuehniella, P. interpunctella, and G. mellonella W chromosomes also possess a large amount of female specific DNA sequences, but differently organized. Our results support the hypothesis of the accelerated molecular divergence of the lepidopteran W chromosomes in the absence of meiotic recombination.</p>\",\"PeriodicalId\":347802,\"journal\":{\"name\":\"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology\",\"volume\":\" \",\"pages\":\"917-30\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s10577-007-1173-7\",\"citationCount\":\"59\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10577-007-1173-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2007/11/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-007-1173-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2007/11/7 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 59
摘要
大多数鳞翅目具有WZ/ZZ性染色体系统。我们比较了Pyralidae科4个代表物种(Ephestia kuehniella、Cadra cautella、Plodia interpunctella和Galleria mellonella)的W染色体结构,发现它们的粗线二价体的分辨率比中期染色体高得多。在每个物种中,我们用激光显微解剖雌性多倍体核的w -染色质制备了w染色体染色探针。将Ephestia W-探针与其他pyralids的染色体进行交叉杂交,以检测其W染色体的共同部分,而物种特异性W-探针则鉴定各自的W染色体。这一所谓的zoofish结果显示,kuehniella的w染色体区域与另外两种pyralides, C. cautella和P. interpunctella有部分同源性,但与G. mellonella几乎没有同源性。结果与物种间的系统发育关系一致。我们还进行了比较基因组杂交,结果表明,C. cautella的W染色体主要由两性共有的重复DNA组成,但在W染色体上积累,而E. kuehniella, P. interpunctella和G. mellonella的W染色体也具有大量的雌性特异性DNA序列,但组织方式不同。我们的结果支持鳞翅目W染色体在没有减数分裂重组的情况下加速分子分化的假设。
Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera).
Most Lepidoptera have a WZ/ZZ sex chromosome system. We compared structure of W chromosomes in four representatives of the family Pyralidae--Ephestia kuehniella, Cadra cautella, Plodia interpunctella, and Galleria mellonella--tracing pachytene bivalents which provide much higher resolution than metaphase chromosomes. In each species, we prepared a W-chromosome painting probe from laser-microdissected W-chromatin of female polyploid nuclei. The Ephestia W-probe was cross-hybridized to chromosomes of the other pyralids to detect common parts of their W chromosomes, while the species-specific W-probes identified the respective W chromosome. This so-called Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. The results were consistent with phylogenetic relationships between the species. We also performed comparative genomic hybridization, which indicated that the W chromosome of C. cautella is composed mainly of repetitive DNA common to both sexes but accumulated in the W chromosome, whereas E. kuehniella, P. interpunctella, and G. mellonella W chromosomes also possess a large amount of female specific DNA sequences, but differently organized. Our results support the hypothesis of the accelerated molecular divergence of the lepidopteran W chromosomes in the absence of meiotic recombination.