Lamins A and C are present in the nuclei of early porcine embryos, with lamin A being distributed in large intranuclear foci.

Helen A Foster, Paula Stokes, Katherine Forsey, Henry J Leese, Joanna M Bridger
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Abstract

Gametogenesis and embryogenesis are dynamic developmental stages marked by extensive modifications in the organization of the genome and nuclear architecture. In the literature it is conveyed that only B-type lamins are required in these early stages of development and that A-type lamins are not present or required until differentiation of specific cell types associated with specialized tissue is initiated. To assess the presence of nuclear structures that are putatively involved in genome regulation, we investigated the distribution of lamin proteins throughout the early stages of porcine embryonic development, using testes tissue sections, oocytes and in-vitro fertilized (IVF) porcine embryos and employing anti-lamin antibodies. We have shown that anti-lamin A staining is present at the one-cell, two-cell, four-cell, and six- to eight-cell stages of early porcine embryo development, but diminishes at the morulae and blastocyst stages. Large intranuclear anti-lamin A foci are prominent in the early preimplantation stages. Both anti-lamin A/C and anti-lamin B staining were clearly present in all embryonic stages. Immature porcine oocytes revealed lamin rings using the monoclonal anti-lamin A/C antibody and many immature oocytes exhibited a pale rim staining pattern with anti-lamin A antibody. A-type lamins were not observed in sperm precursor cells. Thus, we have shown that A-type lamins and B-type lamins are present at the nuclear envelope in very early porcine embryos and that lamin A is also found in large intranuclear aggregates in two-cell to eight-cell embryos but is lacking from later embryonic stages.

层粘连蛋白A和层粘连蛋白C存在于早期猪胚胎的细胞核中,层粘连蛋白A分布在较大的核内灶。
配子发生和胚胎发生是动态的发育阶段,其特征是基因组组织和细胞核结构的广泛改变。在文献中,只有b型层粘连蛋白在这些发育的早期阶段是必需的,而a型层粘连蛋白在与特化组织相关的特定细胞类型分化开始之前不存在或不需要。为了评估被认为参与基因组调控的核结构的存在,我们使用睾丸组织切片、卵母细胞和体外受精(IVF)猪胚胎,并使用抗纤层蛋白抗体,研究了纤层蛋白在猪胚胎发育早期阶段的分布。我们已经表明,抗纤层蛋白A染色存在于早期猪胚胎发育的单细胞、二细胞、四细胞和六至八细胞阶段,但在胚泡和胚泡阶段减少。大的核内抗核纤层蛋白A病灶在植入前早期非常突出。抗层粘连蛋白A/C和抗层粘连蛋白B染色在胚胎各阶段均明显存在。单克隆抗lamin A/C抗体在未成熟的猪卵母细胞中显示出纤层蛋白环,许多未成熟卵母细胞在抗纤层蛋白A抗体下显示出苍白的边缘染色模式。精子前体细胞中未见a型纤层蛋白。因此,我们已经证明,A型层粘连蛋白和b型层粘连蛋白存在于非常早期的猪胚胎的核膜中,并且层粘连蛋白A也存在于2细胞至8细胞胚胎的大核内聚集体中,但在胚胎后期缺乏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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