Phytomedicine Plus最新文献

{"title":"Cytotoxic and anti-migratory effects of Ocimum gratissimum L. essential oil on prostate and cervical cancer cells in vitro","authors":"Laetitia Liz Coulibaly ,&nbsp;Bagora Bayala ,&nbsp;Julio Buñay ,&nbsp;Aminata Marie Simone Amsalet Traoré ,&nbsp;Pengdwendé Fabienne Ingrid Zongo ,&nbsp;Marc Donald Wilfried Adico ,&nbsp;Gilles Figueredo ,&nbsp;Jean-Marc Lobaccaro ,&nbsp;Jacques Simpore","doi":"10.1016/j.phyplu.2025.100818","DOIUrl":"10.1016/j.phyplu.2025.100818","url":null,"abstract":"<div><div>Essential oil of <em>Ocimum gratissimum</em> L. (<em>O. gratissimum</em>) from Burkina Faso was evaluated for its antioxidant activity and for the first time, its cytotoxic properties, and effects on the cell cycle of LNCaP and HeLa cells, respectively derived from prostate and cervix tumors. Gas chromatography-flame ionization detection and gas chromatography-mass spectrometry identified 53 compounds, among them thymol (38.10 %) as the major component, which could explain the antiradical properties and cytotoxic activity of the essential oil on LNCaP and HeLa cells. The essential oil also exhibited antimigratory activity on the LNCaP cells with the reduction of their proliferation by inducing cell cycle arrest in the G0/G1 phase. While these results need to be confirmed in preclinical models, they support the potential use of cell cycle arrest of <em>O. gratissimum</em> essential oil as an antitumor agent, particularly in prostate cancer.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100818"},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"9th surgery decoction alleviates psoriasis-like inflammation through the downregulation of angiogenesis induced by HIF-1α and the EGFR-PI3K-AKT-mTOR pathways","authors":"Shengjie Zhu ,&nbsp;Xinyi Feng ,&nbsp;Ge Yan ,&nbsp;Xin Liu ,&nbsp;Yuanran Chen ,&nbsp;Likun Zhang ,&nbsp;Kan Ze","doi":"10.1016/j.phyplu.2025.100817","DOIUrl":"10.1016/j.phyplu.2025.100817","url":null,"abstract":"<div><h3>Background</h3><div>9th Surgery Decoction (9SD) is a long clinically-proved herbal mixture against inflammatory skin diseases like psoriasis, eczema and acne. There still lacks researches revealing the role of 9SD in treatment of psoriasis. This work investigated the mechanism of 9SD in psoriasis treatment.</div></div><div><h3>Methods</h3><div>The main components of the 9SD were analyzed using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Network pharmacology was employed to identify potential targets and pathways. Psoriasis-like inflammation was induced in human keratinocytes and human umbilical vein endothelial cells using a cytokine cocktail, and the therapeutic effects of the decoction were validated <em>in vitro</em>. Imiquimod-induced psoriasis-like dermatitis in mice was used to assess the effects <em>in vivo</em>. Histopathological changes, gene expression, and protein levels were analyzed using various techniques, including real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and western blotting.</div></div><div><h3>Results</h3><div>The study revealed that 9SD contains multiple active components that target various pathways involved in psoriasis. It was found to exert anti-inflammatory effects by reducing the levels of cytokines such as epidermal growth factor, vascular endothelial growth factor, and tumor necrosis factor-alpha. Additionally, the decoction inhibited angiogenesis by downregulating hypoxia-induced factor 1-alpha and the epidermal growth factor receptor-phosphoinositide 3-kinase-protein kinase B-mechanistic target of rapamycin signaling pathways.</div></div><div><h3>Conclusions</h3><div>The 9SD demonstrates significant anti-psoriatic effects by targeting inflammation and angiogenesis through multiple pathways. These findings suggest that the decoction could be a promising therapeutic option for psoriasis, particularly in modulating the vascular and inflammatory aspects of the disease.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100817"},"PeriodicalIF":0.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the antibacterial and efflux inhibitory activity of trans-cinnamic acid in Staphylococcus aureus: Experimental assays and in silico modeling","authors":"Nair Silva Macêdo ,&nbsp;Zildene de Sousa Silveira ,&nbsp;Débora Menezes Dantas ,&nbsp;Cristina Rodrigues dos Santos Barbosa ,&nbsp;Cícera Datiane de Morais Oliveira-Tintino ,&nbsp;Saulo Relison Tintino ,&nbsp;Gabriel Gonçalves Alencar ,&nbsp;Gustavo Miguel Siqueira ,&nbsp;Thaís Ferreira da Silva ,&nbsp;Márcia Machado Marinho ,&nbsp;Matheus Nunes da Rocha ,&nbsp;Henrique Douglas Melo Coutinho ,&nbsp;Emmanuel Silva Marinho ,&nbsp;Hélcio Silva dos Santos ,&nbsp;Francisco Assis Bezerra da Cunha","doi":"10.1016/j.phyplu.2025.100816","DOIUrl":"10.1016/j.phyplu.2025.100816","url":null,"abstract":"<div><h3>Background</h3><div><em>Staphylococcus aureus</em> develops bacterial resistance using membrane transport systems that perform the active efflux of antimicrobial agents, allowing its survival and growth.</div></div><div><h3>Objective</h3><div>This study evaluated the antibacterial activity of <em>trans-</em>cinnamic acid and its ability to modify the antibiotic activity and inhibit the efflux pump mechanism present in the 1199B strain of <em>S. aureus</em> (NorA overexpressed).</div></div><div><h3>Methodology</h3><div>The permeability of <em>trans-</em>cinnamic acid through the cytoplasmic membrane of <em>S. aureus</em> was also evaluated. In silico assays were used to evaluate the molecular interactions of this compound with the efflux protein NorA.</div></div><div><h3>Results</h3><div><em>Trans-</em>cinnamic acid did not show direct antibacterial activity against the 1199B strain of <em>S. aureus</em>. However, when combined with norfloxacin, it potentiated the antibiotic activity and reduced the MIC of norfloxacin. In addition, <em>trans</em>-cinnamic acid potentiated the effect of EtBr, reducing its MIC from 128 µg/mL to 64 µg/mL. The evaluated compound also increases membrane permeability in <em>S. aureus</em> strains, suggesting that this is one of the mechanisms of action involved in its activity. Molecular docking simulations showed that <em>trans</em>-cinnamic acid can act as an inhibitor of the NorA efflux pump due to its hydrophobic interactions with residues Tyr225 and Phe303. Pharmacokinetic descriptors showed that the compound presents good cell permeability due to the balance between lipophilicity and polarity favored by the presence of a deprotonated carboxylate group. Conclusion: In conclusion, <em>trans-</em>cinnamic acid has promising potential as an inhibitor of the resistance mechanism present in <em>S. aureus</em> and could act as a possible antibiotic adjuvant.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100816"},"PeriodicalIF":0.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early intervention of baicalin suppresses obesity-induced adipose tissue fibrotic remodeling through Interfering NF-κB/HIF-1ɑ/GRK2 Signaling Pathway","authors":"Ying Zeng ,&nbsp;Wenting Li ,&nbsp;Tingting Ma ,&nbsp;Honglei Zhang ,&nbsp;Jiaxing Xu ,&nbsp;Kang Liu","doi":"10.1016/j.phyplu.2025.100815","DOIUrl":"10.1016/j.phyplu.2025.100815","url":null,"abstract":"<div><div>Obesity-induced adipose tissue (AT) fibrosis is difficult to reverse, indicating the importance of early intervention. G protein-coupled receptor kinase 2 (GRK2) is a potential regulator of energy metabolism. Baicalin can improve insulin sensitivity and metabolic homeostasis. However, their impacts on AT fibrosis have not been deciphered. This study aims to uncover the influence of early baicalin intervention on GRK2-related AT fibrotic remodeling under lipid overload. Mice were fed a high-fat diet (HFD) for 8 weeks and received baicalin or metformin during the whole period. 3T3-L1 adipocytes were pre-treated with baicalin, metformin, or pharmacological inhibitors before exposure to palmitic acid (PA) or hypoxia for 20 h. Sometimes, Cells were transfected with siRNA or plasmid DNA specific for GRK2. Results showed that increased GRK2 in AT was observed early after HFD feeding. GRK2 silencing in adipocytes attenuated the profibrotic activation and improved insulin sensitivity and adipokine secretion, but GRK2 overexpression had the opposite effect. Baicalin treatment resulted in the downregulation of GRK2 in 3T3-L1 adipocytes and epididymal AT from the early stage after lipid overload, prevented the profibrotic response, and ameliorated systemic and local insulin resistance. In addition, the negative regulation of GRK2 by baicalin was associated with reduced NF-κB phosphorylation and HIF-1ɑ abundance. Collectively, baicalin prevents HFD-induced AT fibrosis and ameliorates insulin resistance by interfering NF-κB/HIF-1ɑ/GRK2 signaling pathway. This finding provides a better insight into the regulatory effect of baicalin on AT homeostasis and reinforces its promising role in managing obesity-related diseases.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100815"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on Ayurvedic plants and isolation of their phytoconstituents","authors":"Vipin Kumar Jain ,&nbsp;Vijay Kumar ,&nbsp;Ch.V. Narasimhaji","doi":"10.1016/j.phyplu.2025.100813","DOIUrl":"10.1016/j.phyplu.2025.100813","url":null,"abstract":"<div><div>This review discusses the medical and pharmaceutical significance of Ayurvedic plants with a focus on their relevance in daily life. A brief overview of common techniques employed for the isolation of marker compounds (phytoconstituents) is provided along with a detailed examination of the marker compound libraries derived from various Ayurvedic plants.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100813"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanism of phytochemical compounds in mitigating hypertension","authors":"Putri Cahaya Situmorang ,&nbsp;Syahputra Wibowo ,&nbsp;Masitta Tanjung ,&nbsp;Raden Jokokuncoroningrat Susilo ,&nbsp;Ananda ,&nbsp;Rizal Mukra ,&nbsp;Alexander Patera Nugraha ,&nbsp;Wida Akasah","doi":"10.1016/j.phyplu.2025.100812","DOIUrl":"10.1016/j.phyplu.2025.100812","url":null,"abstract":"<div><h3>Background</h3><div>Hypertension is a leading risk factor for cardiovascular diseases and mortality worldwide, particularly in low- and middle-income countries. While conventional antihypertensive therapies exist, their adverse effects and cost limit accessibility, prompting the exploration of safer, plant-based alternatives.</div></div><div><h3>Purpose</h3><div>This review aims to comprehensively examine the therapeutic potential of phytochemicals in the management of hypertension by elucidating their mechanisms of action across multiple molecular pathways.</div></div><div><h3>Method</h3><div>A comprehensive literature review was conducted using databases including PubMed®, Google Scholar, ScienceDirect®, and Scopus. The analysis focused on phytochemicals affecting vascular smooth muscle cells (VSMCs), oxidative stress (ROS/NO), the renin–angiotensin system (RAS), NF-κB signaling, endothelial dysfunction, and prostacyclin (PGI₂) pathways.</div></div><div><h3>Results</h3><div>Numerous phytochemicals, including quercetin, resveratrol, apigenin, and ursolic acid, demonstrate antihypertensive effects by targeting key signaling cascades involved in vascular remodeling, inflammation, and oxidative stress. Emerging evidence supports the role of nanotechnology in enhancing their bioavailability and therapeutic efficacy. However, challenges remain regarding bioavailability, standardization, clinical validation, and potential drug interactions.</div></div><div><h3>Conclusion</h3><div>Phytochemicals offer a multi-targeted and sustainable strategy for hypertension management. Future research should prioritize clinical trials, pharmacokinetics, personalized approaches, and advanced delivery systems to translate preclinical findings into clinical practice and improve outcomes in hypertensive populations.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100812"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated bioinformatics and network pharmacology analysis to reveal the therapeutic targets and potential molecular mechanism of Traditional Chinese Medicine Lianhua-Qingwen capsule on children's influenza","authors":"Lijuan Fang ,&nbsp;Caiyan Li ,&nbsp;Haimai Ding ,&nbsp;Jinjin Tao","doi":"10.1016/j.phyplu.2025.100807","DOIUrl":"10.1016/j.phyplu.2025.100807","url":null,"abstract":"<div><div>Background Influenza annually affects 5%-10% of adults and up to 20%-30% of children worldwide, with pediatric populations being more susceptible to severe complications and even mortality due to their immature immune systems. Traditional Chinese Medicine (TCM) demonstrates unique advantages in influenza treatment through multi-target mechanisms inhibiting viral replication and modulating host immunity. As a clinically prevalent TCM formulation for respiratory infections, the molecular mechanisms underlying Lianhua Qingwen Capsule's (LHQW) therapeutic effects against pediatric influenza remain elusive.</div><div>Purpose and methods This study focuses on children and combines network pharmacology, molecular docking, and pediatric influenza target data to reveal the molecular mechanism of LHQW therapy for influenza virus infection, breaking through the limitations of adult influenza mechanism research. Through integrating childhood influenza targets with LHQW active ingredients, a \"disease drug\" interaction network was constructed, and a \"principal component-micro regulation\" synergistic model of herbal formulas was established, providing a digital research paradigm for analyzing the compatibility principles of herbal formulas</div><div>Result Network pharmacology and molecular docking experiments have shown that LHQW mainly exerts therapeutic effects through the TNF and TLR4 signaling pathways. <em>In vitro</em> experiments have shown that LHQW has a significant inhibitory effect on the virus, with IC₅₀ and TC₅₀ values of 6.68 ± 1.03 μM and 9.97 ± 1.26 μM, respectively. <em>In vivo</em> experiments have confirmed that LHQW can improve the survival rate of mice, promote weight recovery, reduce viral titers, enhance antiviral antibody levels, and reduce lung index, inflammatory cytokine levels, and lung injury. Moreover, LHQW improves inflammation and oxidative stress by inhibiting the expression of TLR4 and MyD88, while reducing lung tissue cell apoptosis.</div><div>Conclusion Notably, we pioneer in elucidating LHQW's dual-target mechanism that concurrently disrupts viral lifecycles and modulates host factors, thereby opening new avenues for developing antiviral therapeutics.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100807"},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity","authors":"Usharani Saminathan ,&nbsp;Anuradha Ramamoorthy ,&nbsp;Ambiga Somasundaram ,&nbsp;Sivasankari Sekar ,&nbsp;Sivagurunathan Paramasivam ,&nbsp;Pasiyappazham Ramasamy","doi":"10.1016/j.phyplu.2025.100810","DOIUrl":"10.1016/j.phyplu.2025.100810","url":null,"abstract":"<div><h3>Introduction</h3><div>The goal of this study was to find out if kaempferol, a compound isolated from the <em>Wattakaka volubilis</em> plant, could protect the liver of rats that were given aluminum sulfate.</div></div><div><h3>Methods</h3><div>Kaempferol, a bioflavonoid, was isolated from the leaves of W. volubilis methanolic extract (MEWV) and characterized by using the High-performance thin-layer chromatography (HPTLC)technique. The liver protective activities of MEWV against aluminum poisoning were investigated in male albino rats, as their protective efficacy was assessed in addition to that of the silymarin standard. The oral administration of silymarin (25 mg/kg), kaempferol (10 mg/kg), and MEWV (200 mg/kg) was administered continuously for 14 days. The body and liver weights as well as serum enzymes like alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were calculated.</div></div><div><h3>Results</h3><div>Advanced techniques successfully isolated and confirmed the bioactive constituent kaempferol from MEWV. When kaempferol and MEWV were given to rats that had been poisoned with aluminum, their body mass index went up significantly (<em>p</em> &lt; 0.001) and the relative weight of their liver and serum marker enzymes went down significantly (<em>p</em> &lt; 0.001) compared to animals that had only been poisoned and analyzed the comparative action of the extract and isolated compound using silymarin, the standard drug. Kaempferol showed better protection against aluminum-induced hepatotoxicity compared to MEWV treatment<strong>.</strong></div></div><div><h3>Conclusion</h3><div>In conclusion, the results showed that kaempferol and MEWV reduced the damage done to the liver by aluminum sulfate by protecting cells, the liver, and protecting antioxidants.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100810"},"PeriodicalIF":0.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ayurvedic management of non-alcoholic fatty liver disease with obesity and rheumatoid arthritis– A case report","authors":"Amit Nakanekar,&nbsp;Rashmi Jaiswal","doi":"10.1016/j.phyplu.2025.100787","DOIUrl":"10.1016/j.phyplu.2025.100787","url":null,"abstract":"<div><h3>Background</h3><div>Non-alcoholic fatty Liver Disease (NAFLD) is one of the leading causes of chronic liver diseases, with a 25 % frequency worldwide. NAFLD is characterized by a complex \"multi-hit\" pathogenesis and spectrum of disorders. In Ayurveda, it is considered under <em>Yakrit vikar</em> (∼liver disease) with <em>Medoroga</em> (∼disease caused by excessive fat deposition).</div></div><div><h3>Purpose</h3><div>A 52-year-old non-alcoholic male patient came with complaints of abdominal distension, fatigue, weakness, back pain, bilateral upper limb tingling, striae present over the thigh and abdomen, bilateral shoulder joint stiffness, anorexia, constipation, and pain at the planter region for the last six months. The patient was admitted to a government medical college and was treated with NSAIDs, Antibiotics, antacids, steroids, and some multivitamin tablets for Grade II fatty liver disease. However, the recurrence occurred, so the patient came to Ayurveda for the same complaints with more added pain in all joints.</div></div><div><h3>Study design</h3><div>The patient was treated using <em>Santarpana</em> (∼overconsumption and sedentary lifestyle) and <em>Krumighn Chikitsa</em> (∼ treatment aimed at eliminating parasites). The specialty of this case is that <em>Krumighna</em> treatment worked well in NAFLD, rheumatoid arthritis, and obesity. The patient was treated for around four months with <em>Basti</em> (∼medicated enema using oil and decoction) and Ayurvedic oral medicines.</div></div><div><h3>Result</h3><div>A remarkable improvement was shown in clinical symptoms, weight, and abdomen and pelvis ultrasound findings.</div></div><div><h3>Conclusion</h3><div>further studies must be conducted to obtain more scientific evidence for the efficacy of this Ayurvedic approach.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100787"},"PeriodicalIF":0.0,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant-like effect of Entada Africana Guill. & Perr. (Fabaceae) in mice: Involvement of monoamine neurotransmitters, cortisol, BDNF, and oxidative stress","authors":"M.Y. Bebeji ,&nbsp;A.H. Yaro ,&nbsp;A.R. Abubakar ,&nbsp;M.H. Ahmad ,&nbsp;A.B. Nazifi ,&nbsp;A.A.E. Ahmed","doi":"10.1016/j.phyplu.2025.100809","DOIUrl":"10.1016/j.phyplu.2025.100809","url":null,"abstract":"<div><h3>Background</h3><div><em>Entada Africana</em> is used to treat various disease conditions including stomach ache, cataract, liver disease, epilepsy and depression. Previous research reported its antidepressant properties in acute depression models. However, its activity in chronic model and the associated signaling pathways remain unclear.</div></div><div><h3>Purpose</h3><div>To elucidate the antidepressant-like effect of ethanol leaf extract of <em>Entada Africana</em> (EEEA) in chronic model of depression and the possible mechanism(s).</div></div><div><h3>Methods</h3><div>Limit test was utilized in determining the oral median lethal dose (LD₅₀). To establish the potential roles of monoamine neurotransmitters in the antidepressant action of EEEA, groups of mice were pre-administered prazosin (1 mg/kg), cyproheptadine (3 mg/kg), and haloperidol (0.2 mg/kg), 30 min before administering EEEA (4 mg/kg) or imipramine (10 mg/kg). In a separate experiment, animals were depressed via chronic unpredictable mild stress (CUMS), followed by sucrose preference, open field (OF), and tail suspension (TS) tests. The quantities of cortisol, brain-derived neurotrophic factor (BDNF), superoxide dismutase, and malondialdehyde were estimated. Phytochemical profiling of the extract was also conducted.</div></div><div><h3>Results</h3><div>The oral LD₅₀ was estimated to be <strong>&gt;</strong>2000 mg/kg in mice. Pre-administration of prazosin, cyproheptadine, and haloperidol significantly (<em>p</em> &lt; 0.05) attenuated the antidepressant action of EEEA. In CUMS experiment, EEEA remarkably (<em>p</em> &lt; 0.05) reversed the weight loss, improved sucrose consumption, increased exploration in OF, and shortened the duration of motionlessness. EEEA significantly (<em>p</em> &lt; 0.05) lowered the serum cortisol, increased BDNF concentration, and ameliorated oxidative stress. Flavonoids, saponins, terpenoids and tannins were identified.</div></div><div><h3>Conclusions</h3><div><em>Entada Africana</em> exerted antidepressant-like effect in chronic model of depression, which may be mediated via modifications of monoamine NTs, cortisol, BDNF, and oxidative stress pathways.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 3","pages":"Article 100809"},"PeriodicalIF":0.0,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}