Phytomedicine Plus最新文献

{"title":"Integrating network pharmacology and proteomics to uncover the anti-inflammatory and antioxidant stress mechanisms of Shengbaishao Gancao Decoction in septic acute kidney injury","authors":"Xuan Shang,&nbsp;Wenhuan Chai,&nbsp;Jie Wang,&nbsp;Yawen Wu,&nbsp;Mulin Wang,&nbsp;Juan He,&nbsp;Xiaohui Zhang,&nbsp;Jinru Liu,&nbsp;Jimin Cao,&nbsp;Lijuan Gao","doi":"10.1016/j.phyplu.2026.101024","DOIUrl":"10.1016/j.phyplu.2026.101024","url":null,"abstract":"<div><div>Sepsis-induced acute kidney injury (S-AKI) is a life-threatening disease, which therapeutic strategies are supportive but not effective. Shengbaishao Gancao Decoction (SGD) is a traditional Chinese medicine, which has multiple beneficial effects on a variety of diseases. However, whether SGD has a therapeutic effect on S-AKI is unknown. The present study aims to comprehensively investigate the potential therapeutic effect of SGD on S-AKI and its molecular mechanisms. We used cecal ligation and puncture (CLP) to establish S-AKI model. SGD (22 and 44 g/kg) was orally administrated once a day for seven days prior to modeling. The kidney injury, systemic inflammation, and mitochondrial oxidative stress were measured and compared among different groups. Network pharmacology, proteomic analysis and molecular docking were performed to investigate the underlying pharmacological targets of SGD and the regulatory mechanisms. Administration of SGD decreased kidney injury, mitigated systemic inflammation and alleviated oxidative stress in septic mice. Network pharmacology, proteomic analysis and molecular docking predicted that SGD might protect against S-AKI by alleviating systemic inflammation and increasing antioxidant function through PI3K-AKT signaling pathway.</div></div><div><h3>Significance</h3><div>The development of effective therapeutic agents for S-AKI remains a critical unmet medical need. Although SGD has been widely recognized for its anti-inflammatory and renal properties, its therapeutic potential mechanisms in S-AKI remains unclear. In this study, we employed an integrative approach combining network pharmacology, proteomics, and molecular docking to unravel the protective mechanisms of SGD against S-AKI. These findings not only deepen the understanding of traditional Chinese medicine in addressing complex diseases but also provide a scientifically validated therapeutic strategy for the treatment of S-AKI.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101024"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidimensional pharmacological activities of Moringa stenopetala: Evidence from in vivo and in vitro experimental studies","authors":"Gudisa Bereda","doi":"10.1016/j.phyplu.2026.101022","DOIUrl":"10.1016/j.phyplu.2026.101022","url":null,"abstract":"<div><h3>Background</h3><div><em>Moringa stenopetala</em> is a nutritionally rich medicinal plant widely used in traditional medicine for managing metabolic, infectious, inflammatory, and chronic diseases. Despite its extensive ethnomedicinal use, its pharmacological effects and safety profile are reported variably across experimental and regulatory studies.</div></div><div><h3>Objective</h3><div>To summarize the pharmacological activities, safety profile, and experimental evidence of <em>Moringa stenopetala</em> based on in vitro and in vivo studies.</div></div><div><h3>Methods</h3><div>A narrative synthesis of published experimental studies, including in vitro assays, in vivo animal models, toxicity studies, and chemical analyses, was performed. Studies evaluating metabolic, antimicrobial, cardiovascular, neurological, anticancer, reproductive, and toxicological outcomes were included. The findings were synthesized qualitatively.</div></div><div><h3>Results</h3><div>This review demonstrated that <em>Moringa stenopetala</em> possesses broad pharmacological activities in both in vitro and in vivo models. Leaf extracts at 250–500 mg/kg for 14–28 days significantly reduced blood glucose, improved lipid profiles, and restored pancreatic β-cells in diabetic animals. Antioxidant studies showed strong radical-scavenging activity and increased levels of endogenous antioxidant enzymes. Antimicrobial studies demonstrated potent antibacterial and antifungal effects, with MIC values as low as 0.31 mg/mL. Antiparasitic studies using 400 mg/kg for 7 days reduced parasitemia and improved survival in infected mice. Cardiovascular studies using 10–1000 mg/kg extracts showed antihypertensive, vasorelaxant, diuretic, and natriuretic effects. Additional studies reported analgesic, anti-inflammatory, anticonvulsant, neuroprotective, antidiarrheal, and anticancer activities. Toxicological evaluations showed favorable safety profiles, with LD50 values &gt;5000 mg/kg and minimal organ toxicity.</div></div><div><h3>Conclusion</h3><div><em>Moringa stenopetala</em> possesses broad pharmacological potential supported mainly by preclinical evidence. However, toxicity studies indicate possible dose-dependent safety concerns, particularly at high exposure levels. The absence of human clinical confirmation highlights the need for further translational and clinical research.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101022"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of the fertility-enhancing effects of Garcinia kola and its metabolites: Evidence from in vivo rodent studies","authors":"Khadijah Bolanle Ayoade-Suleiman,&nbsp;Oluwaseun Emmanuel Olatunji,&nbsp;Ekom Monday Etukudo","doi":"10.1016/j.phyplu.2026.101025","DOIUrl":"10.1016/j.phyplu.2026.101025","url":null,"abstract":"<div><h3>Background</h3><div>Infertility affects 8–12% of couples globally, with a higher burden in developing regions where access to conventional fertility treatments is limited. This has fueled growing interest in complementary and alternative medicines such as medicinal plants. <em>Garcinia kola</em> (bitter kola), a native plant in West and Central Africa, is traditionally used to enhance reproductive health. This systematic review examined the potential of <em>G. kola</em> and its metabolites on fertility and their mechanisms of action.</div></div><div><h3>Method</h3><div>A PRISMA 2020 guided systematic review was conducted using databases including PubMed, Scopus, Web of Science, and Google Scholar, a grey literature. Peer-reviewed articles written in English involving rodent models that evaluated fertility outcomes were included. A deeper insight into the safety and toxicological profile of <em>G. kola</em> metabolites was provided by performing <em>in silico</em> screening using the SwissADME and pkCSM web servers.</div></div><div><h3>Result</h3><div>Thirty-four articles met the inclusion criteria. Most studies were conducted in Nigeria (91.18%), followed by South Africa and Egypt. <em>Kolaviron</em> and Hydroxybiflavanonol were the identified phytocompounds in the articles reviewed. These antioxidant metabolites demonstrated significant reproductive benefits by improving sperm quality, testicular histoarchitecture, and restoring reproductive hormone levels.</div></div><div><h3>Conclusion</h3><div>The review identifies kolaviron and Hydroxybiflavanonol as <em>G. kola’</em>s metabolites with the potential of improving reproductive health. Nonetheless, due to the varied pharmacokinetic and toxicological profiles of these metabolites, further clinical studies are needed to validate their efficacy, establish optimal dosing, and ensure safety in human populations.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101025"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective potential of centella asiatica, cinnamon, and spirulina extracts in traumatic brain injury: a biomarker-based approach using BDNF, TNF-α, and NGF","authors":"Rohadi,&nbsp;Dyah Purnaning,&nbsp;Herpan Syafii Harahap,&nbsp;Bambang Priyanto,&nbsp;Januarman,&nbsp;Lina Permatasari,&nbsp;Legis Ocktaviana Saputri,&nbsp;Angeline Rufina,&nbsp;Dwi Rahmat","doi":"10.1016/j.phyplu.2026.101031","DOIUrl":"10.1016/j.phyplu.2026.101031","url":null,"abstract":"<div><h3>Background</h3><div>Traumatic brain injury (TBI) continues to be a significant worldwide health issue, with its incidence increasing, especially in low- and middle-income countries. The complex TBI mechanism involves both primary and secondary injury processes mediated by excitotoxicity, oxidative stress, and neuroinflammation, leading to damage of the blood–brain barrier (BBB) and neuronal dysfunction. Biomarkers such as brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and tumor necrosis factor-alpha (TNF-α) have been widely explored to assess neuronal survival, neuroplasticity, and the degree of neuroinflammation in TBI. In recent years, increasing attention has been directed toward plant-derived compounds with neuroprotective potential. <em>Centella asiatica (CA)</em>, cinnamon, and spirulina represent promising candidates because their ability as an antioxidant, anti-inflammatory, and neurotrophic factors. Experimental studies have demonstrated that bioactive compounds such as asiatic acid in <em>CA</em>, cinnamaldehyde in cinnamon, and phycocyanin in spirulina can cross the BBB and modulate key neuroprotective pathways. These include upregulation of BDNF and NGF, inhibition of TNF-α as an pro-inflammatory cytokines, and attenuation of neuronal damage through reduction of oxidative stress and apoptosis. Furthermore, several in vivo studies using rodent models of TBI support the efficacy of these natural extracts in enhancing histopathological recovery and modulating molecular responses in the acute phase of brain injury. This review highlights the therapeutic potential of <em>CA</em>, cinnamon, and spirulina as adjunctive treatments for TBI by focusing on their mechanisms of action through biomarker-based approaches, particularly targeting BDNF, NGF, and TNF-α. Continued exploration of these natural compounds could contribute to the development of novel, accessible, and effective treatment for neuroprotection and recovery in TBI.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101031"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148735569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICD-11–aligned Chinese medicine pattern classification in stage IV lung adenocarcinoma: reproducibility and prognostic assessment in two prospective real-world cohorts","authors":"Huiru Guo,&nbsp;Hegen Li,&nbsp;Jan P.A. Baak","doi":"10.1016/j.phyplu.2026.101028","DOIUrl":"10.1016/j.phyplu.2026.101028","url":null,"abstract":"<div><h3>Background</h3><div>Internationally standardised Chinese medicine pattern classification is essential for interoperable integrative oncology research. The WHO ICD-11 Traditional Medicine Module provides a harmonised framework. However, the reproducibility and prognostic value of ICD-11-aligned Chinese medicine (CM) pattern classification in stage IV lung adenocarcinoma (LUAD-IV) remain uncertain across evolving systemic therapy eras.</div></div><div><h3>Methods</h3><div>Two prospective real-world cohorts of newly diagnosed LUAD-IV patients from different therapeutic eras were analysed (2009–2018, <em>n</em> = 412; 2020–2024, <em>n</em> = 133).</div><div>Eligible patients had ECOG performance status 0–1, complete survival follow-up data, and received systemic anticancer therapy plus Chinese herbal medicine from baseline.Baseline patterns were assigned using the China Association of Chinese Medicine (CACM) criteria and mapped to ICD-11 Traditional Medicine codes. Interobserver agreement was assessed in 50 randomly selected cases (Cohen's κ). Overall survival was analysed using Kaplan–Meier methods and multivariable Cox regression with time-dependent treatment covariates to address measured confounding and immortal-time bias.</div></div><div><h3>Results</h3><div>Predominant baseline patterns were Lung–Spleen Qi deficiency (48% vs 37%), Qi–Yin deficiency (46% vs 26%), and Lung–Kidney deficiency (4%vs 32%) in the earlier and contemporary cohorts, respectively. Interobserver agreement was substantial (κ = 0.64 and κ = 0.68). Median overall survival increased from 24 to 48 months. Age ≥68 years, absence of EGFR-TKI or anti-VEGF therapy, and stage IVB disease independently predicted poorer survival, whereas baseline Chinese medicine patterns did not.</div></div><div><h3>Conclusions</h3><div>ICD-11-aligned CACM pattern classification is reproducible across therapeutic eras but does not independently predict survival in LUAD-IV, supporting its use for standardised reporting and treatment individualisation rather than prognostic stratification.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101028"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148735572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible plant-derived exosomes and microRNA-like molecules: A cross-kingdom regulatory network in diabetes immunometabolism","authors":"Satria Rafi Ratmandhika,&nbsp;Michael Pudjihartono,&nbsp;Nicholas Pudjihartono,&nbsp;Felix Zulhendri,&nbsp;Edwin Hadinata,&nbsp;Raymond Rubianto Tjandrawinata,&nbsp;Dananti Kusumawindani,&nbsp;Wiharjo Hadisuwarno,&nbsp;Sovia Salamah,&nbsp;Antonello Santini,&nbsp;Fahrul Nurkolis","doi":"10.1016/j.phyplu.2026.101029","DOIUrl":"10.1016/j.phyplu.2026.101029","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) is increasingly recognized as a chronic immunometabolic disorder driven by persistent inflammation and dysregulated glucose metabolism. Conventional nutrition paradigms, which focus primarily on macro- and micronutrients, fail to account for regulatory bioactive molecules that may influence host gene expression. Emerging evidence highlights edible plant-derived exosome-like nanoparticles (ELNs) as novel carriers of microRNA-like molecules capable of mediating cross-kingdom communication. This review synthesizes current knowledge on the biogenesis, molecular cargo, gastrointestinal stability, and systemic distribution of plant ELNs, emphasizing their role in modulating immunometabolic pathways relevant to diabetes. Preclinical evidence suggests that certain plant ELNs may retain structural integrity during gastrointestinal transit and may be internalized by intestinal cells and gut microbiota, although the efficiency and physiological relevance of these processes in humans remain uncertain. These molecules have been reported to regulate key targets involved in insulin signaling (e.g., IRS/PI3K/AKT), inflammation (e.g., NF-κB, NLRP3 inflammasome), and lipid metabolism. Functionally, plant ELNs may enhance insulin sensitivity, suppress pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and promote macrophage polarization toward anti-inflammatory phenotypes. Additionally, they influence host metabolism indirectly via microbiome-mediated pathways. However, evidence supporting cross-kingdom RNA transfer in humans remains limited, and important questions regarding bioavailability, reproducibility, and physiological relevance are still unresolved. Overall, plant-derived ELNs represent an emerging area of research that expands current understanding of diet-derived gene-regulatory signals. Future integration of multi-omics approaches and artificial intelligence may facilitate the development of hypothesis-driven precision nutrition approaches, pending robust validation in human studies</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101029"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative effect of naringenin against acetaminophen-induced hepatotoxicity in rats: Role of oxidative stress, apoptosis, and Inflammation","authors":"Mariam Ehab Khaled,&nbsp;Sherin Ibrahim Ibrahim,&nbsp;Mohamed Ismail Mostafa","doi":"10.1016/j.phyplu.2026.101027","DOIUrl":"10.1016/j.phyplu.2026.101027","url":null,"abstract":"<div><h3>Overview</h3><div>Oxidative stress is a primary driver of acetaminophen (APAP) hepatotoxicity during an overdose. This study evaluated the protective potential of Naringenin (NGN), a citrus-derived flavonoid, against a toxic dose of APAP. Male Sprague-Dawley rats were pre-treated with NGN at doses of 50, 75, and 100 mg/kg before the administration of 600 mg/kg of APAP. Silymarin was utilized as a positive control.</div></div><div><h3>Biochemical restoration</h3><div>NRN treatment resulted in a significant, dose-dependent reduction in ALT, AST, and ALP levels.</div></div><div><h3>ALT recovery</h3><div>As the dosage increased, ALT levels improved from 27.50 to 24.50 U/L. The 100 mg/kg dose effectively restored ALT to the normal baseline of 25.0 U/L.</div></div><div><h3>Protein synthesis</h3><div>NGN prevented the characteristic albumin depletion, maintaining albumin levels between 3.75 and 3.90 g/dL, values statistically comparable to the healthy control group. <u>Antioxidant and Oxidative Stress Profiles:</u> NGN demonstrated a robust capacity to neutralize oxidative damage, with the 100 mg/kg dose acting as the most effective concentration.</div></div><div><h3>Enzymatic defense</h3><div>At the highest dose, SOD (40.70), TAC (65.70), and GST (38.90) levels were statistically indistinguishable from those of healthy rats.</div></div><div><h3>Lipid peroxidation</h3><div>All NGN dosages successfully reduced MDA levels (a marker of cellular oxidative damage) to within normal ranges.</div></div><div><h3>Anti-inflammatory and anti-apoptotic mechanisms</h3><div>The 100 mg/kg dose significantly modulated the molecular pathways responsible for cell death and inflammation:</div></div><div><h3>Immunoreactivity</h3><div>There was a profound decrease in COX-2 (94.17%) and caspase-3 (98.19%) expression. Furthermore, only mild immunoreactivity for IL-6 and VEGF was observed in a limited number of hepatocytes. Gene and Protein Expression: Western blot and mRNA analysis revealed that NGN significantly downregulated Bax, P53, TLR2, and TLR4, while supporting the expression of the anti-apoptotic protein Bcl2.</div></div><div><h3>Conclusion</h3><div>In summary, Naringenin exerts a potent hepatoprotective effect against APAP-induced acute liver injury. It achieves this by mitigating oxidative stress, inhibiting pro-inflammatory and apoptotic signaling pathways (TLRs/Caspase-3), and bolstering the endogenous antioxidant defense system to promote tissue regeneration.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101027"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renoprotective potentials of Basella alba extract against intestinal ischemia–reperfusion–induced renal damage in Wistar rats","authors":"Olubayode Bamidele,&nbsp;Oluwaseyi G. Ola-Bright,&nbsp;Funmilola O. Adelowo,&nbsp;Lawrence D Adedayo,&nbsp;Gideon B. Ojo,&nbsp;Ayodeji F. Ajayi,&nbsp;Ayobami O. Afolabi","doi":"10.1016/j.phyplu.2026.101026","DOIUrl":"10.1016/j.phyplu.2026.101026","url":null,"abstract":"<div><h3>Background</h3><div>Intestinal ischemia-reperfusion injury (IIRI) is a severe disorder which spreads beyond the intestine, triggering secondary injury to organs like the kidney as a result of mucosal barrier disruption, bacterial and endotoxin translocation<em>. Basella alba,</em> which has traditionally been utilized to treat gastrointestinal disorders, ulcers, wounds, kidney stone, diuresis, urinary tract infections, inflammation, and oxidative stress-related ailments, could be effective to prevent kidney damage. This current research investigated the preventive effects of methanolic leaf extract <em>B. alba</em> (MLEBA) on renal injury in rats after IIRI.</div></div><div><h3>Methods</h3><div>Thirty rats were divided into five groups: sham-operated, IIRI alone, two groups pretreated orally with 100 and 200 mg/kg dosages of MLEBA respectively, and a group pretreated with vitamin C (200 mg/kg) for two weeks before IIRI induction. Kidney function indices, oxidative stress and inflammatory markers, apoptotic indices, and histopathological changes were investigated.</div></div><div><h3>Results</h3><div>IIRI elevated serum creatinine, urea, malondialdehyde, myeloperoxidase, interleukin-6, interleukin-1β and TNF-α, while decreasing total protein and antioxidant enzyme activities. Caspase-1, -3, and -9 levels increased significantly. High-dose MLEBA markedly improved renal function, enhanced antioxidant enzyme activity, reduced levels of inflammatory mediators, and suppressed apoptosis, effects comparable to those of vitamin C treatment. Histology confirmed preserved renal architecture in treated groups</div></div><div><h3>Conclusion</h3><div><em>Basella alba</em> leaf extract provides significant renoprotection against IIRI-induced renal injury, most likely by ameliorating inflammatory processes, oxidative stress, and apoptosis supporting its ethnomedicinal value for renal protection.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101026"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating transcriptomics and network pharmacology to explore the mechanism of Rhizoma Corydalis in preventing DOX-induced acute myocardial injury in mice","authors":"Shanlan Li,&nbsp;Jin An,&nbsp;Tong Man,&nbsp;Jie Wang,&nbsp;jiaxuan Xu,&nbsp;Die Hu,&nbsp;Yuke Li,&nbsp;Dongling Liao,&nbsp;Zhenyuan Yang,&nbsp;Ximo Zhou,&nbsp;Bing Xu,&nbsp;Herong Cui,&nbsp;Haimin Lei","doi":"10.1016/j.phyplu.2026.101014","DOIUrl":"10.1016/j.phyplu.2026.101014","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Acute myocardial infarction (AMI) significantly increases the global health and economic burden. Although Western drug therapies have successfully reduced mortality in patients with acute myocardial injury, they are not without drawbacks, including a range of adverse effects. Although Rhizoma Corydalis (YHS) has been used in China for over a thousand years to prevent cardiac conditions, its precise preventive and therapeutic effects on AMI areremain unclear, necessitating further research into its mechanisms of action.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;To assess the therapeutic potential of YHS against AMI, this study seeks to identify its active components and thereby establish its pharmacological basis.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;In this study, an AMI model was established in mice by tail vein injection of doxorubicin (DOX). Network pharmacology and UHPLC-Q Exactive Orbitrap HRMS technology were used to predict the active constituents, target molecules, and mechanisms of action of YHS against AMI. Echocardiography, histopathological examination, and serum biochemical assays were utilized to assess the therapeutic benefits of YHS and its main active component TP on the AMI mice model. The effects of YHS on the signaling pathways and gene expression profile in AMI were investigated by transcriptome analysis. ROS staining, Western blotting, PCR, and immunofluorescence were utilized to validate the mechanisms of YHS in preventing AMI.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;According to our research, DOX-induced changes in body weight and cardiac indices were considerably improved by both YHS and its main active component TP, which also successfully decreased levels of lactate dehydrogenase (LDH) and creatine kinase MB isoenzyme (CK-MB). YHS and TP significantly improved the low EF and FS levels brought on by DOX overdose, according to echocardiography. Myofibrillar disarray, myocardial cell nuclear atrophy, and inflammatory cell infiltration in the heart were all improved by YHS and TP, according to histological study. The NOD-like receptor pathway, which included important proteins like Nox2, TXNIP, NLRP3, and Caspase-1, may be the basis for YHS's ACT-improving mechanism, according to network pharmacology prediction and transcriptomics research. In addition, DHE staining, immunofluorescence detection, PCR, and Western blot experiments demonstrated that YHS improved AMI in mice by inhibiting DOX-activated Nox2, lowering excessive ROS production, suppressing TXNIP translocation, and reducing inflammasome expression (including NLRP3 and Caspase-1).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;According to research, YHS successfully reduced doxorubicin-induced acute myocardial injury by blocking the NOD-like receptor pathway, lowering levels of reactive oxygen species (ROS) and the important oxidative stress gene &lt;em&gt;Cybb&lt;/em&gt;, and decreasing the production of inflammasomes.","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 3","pages":"Article 101014"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148408692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anticancer potential of Citrus spp. essential oils against various human cancer cell lines","authors":"Pramesthi Reitza Navisya Vasall ,&nbsp;Farah Aprisza Sheelmarevaa ,&nbsp;Nandang Permadi ,&nbsp;Fauzan Setiawan ,&nbsp;Riezki Amalia ,&nbsp;Yaya Rukayadi ,&nbsp;Euis Julaeha","doi":"10.1016/j.phyplu.2026.100990","DOIUrl":"10.1016/j.phyplu.2026.100990","url":null,"abstract":"<div><div>Conventional cancer treatments, such as chemotherapy and radiotherapy, remain the mainstay of therapy but are often associated with severe toxicity and a diminished quality of life for patients. These limitations have increased interest in natural products as sources of novel anticancer compounds and potential adjunctive leads for future development. Natural compounds, particularly those derived from plants, have historically contributed significantly to anticancer drug discovery, with nearly 60% of approved agents originating from natural sources. Among medicinal plants, the <em>Citrus</em> genus has attracted considerable attention for its diverse range of bioactive metabolites, including flavonoids, limonoids, coumarins, and essential oils. <em>Citrus</em> essential oils are particularly rich in monoterpenes, such as d-limonene, β-pinene, and myrcene, which exhibit potent cytotoxic, antiproliferative, and pro-apoptotic effects against various human cancer cell lines. Emerging evidence suggests that these compounds can modulate multiple molecular targets involved in apoptosis, oxidative stress, and tumor progression, suggesting promising bioactivity and mechanistic relevance with potential for future therapeutic development. This review provides an updated and critical overview of the chemical composition, extraction methods, and anticancer mechanisms of <em>Citrus</em> essential oils, with particular emphasis on their molecular modes of action and their prospects for future translational and therapeutic research.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 2","pages":"Article 100990"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}