Phytomedicine Plus最新文献

{"title":"Exploring ethnomedicinal uses and relevance to phytochemical profile, pharmacological activities, and tissue culture of Tecoma stans (L.) Juss. ex Kunth","authors":"Rowida Omar , Marwa Elsbaey , Madiha Hassan , Mohamed Abd El-Salam","doi":"10.1016/j.phyplu.2025.100800","DOIUrl":"10.1016/j.phyplu.2025.100800","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Tecoma stans</em> (L.) Juss. ex Kunth (Bignoniaceae) is a flowering plant native to the Americas and widely naturalized in tropical and subtropical regions. It has been traditionally used to treat diabetes, arthritis, and urinary disorders, with additional benefits including anti-inflammatory, antimicrobial, and antioxidant properties.</div></div><div><h3>Aim</h3><div>Our review provides a comprehensive overview on the therapeutic potential of <em>Tecoma stans</em> focusing on its anti-diabetic properties, traditional ethnomedicinal uses, pharmacological activities, mechanisms of action of its key phytochemicals, and the comparison of these findings with results from plant tissue culture studies. Furthermore, the review explores the applications of <em>Tecoma</em> in green biotechnology and tissue culture, emphasizing conservation and sustainable production of bioactive compounds.</div></div><div><h3>Methodology</h3><div>The literature on <em>Tecoma stans</em> was sourced from Google Scholar, PubMed, and ScienceDirect search engines, covering publications from the first report on the topic in 1954 until April 2024. The review focused on studies highlighting the ethnomedicinal uses, pharmacological activities, phytochemical composition, tissue culture, isolated endophytic fungi and relevant preclinical studies. Particular emphasis was placed on delineating the molecular mechanisms of action of isolated bioactive compounds and systematically evaluating the potential biotechnological applications of the plant, with a focus on sustainable production methodologies.</div></div><div><h3>Results</h3><div>Several studies have demonstrated the efficacy of <em>Tecoma stans</em> in managing diabetes, with evidence suggesting that its therapeutic effectiveness is comparable to that of established conventional treatments. Key phytochemicals such as tecostanine, tecomine, anthranilic acid, and gallic acid have been linked to hypoglycemic, anti-inflammatory, antioxidant, and antimicrobial activities. Mechanistic insights suggest that these compounds modulate various biological targets involved in glycemic control and inflammation. While these compounds have shown promising activity against several biological targets, further clinical trials are necessary to fully validate these effects. Preclinical studies indicate low toxicity; however, comprehensive toxicity assessments are still needed to confirm safety for clinical use. Additionally, tissue culture approaches and isolated endophytes have been investigated as sustainable methods for the production of these bioactive secondary metabolites.</div></div><div><h3>Conclusions</h3><div>In summary, the phytochemicals derived from <em>Tecoma stans</em> exhibit significant therapeutic potential, particularly in the management of diabetes and related conditions, without notable side effects. These findings suggest that <em>Tecoma stans</em> could be considered a viabl","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100800"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of flavonoid-rich extracts from Hibiscus sabdariffa leaves on streptozotocin-induced testicular damage in rats","authors":"Basiru Olaitan Ajiboye ,&nbsp;Courage Dele Famusiwa ,&nbsp;John Adeolu Falode ,&nbsp;Daniel Okwukwe Akojuru ,&nbsp;Bamikole Tosin Owolabi ,&nbsp;Adedeji A. Adejumo ,&nbsp;Babatunji Emmanuel Oyinloye ,&nbsp;Olawale Razaq Ajuwon ,&nbsp;Oluwatosin Olalekan Ogedengbe ,&nbsp;Oluwafemi Adeleke Ojo","doi":"10.1016/j.phyplu.2025.100808","DOIUrl":"10.1016/j.phyplu.2025.100808","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the protective effects of flavonoid-rich extracts from <em>Hibiscus sabdariffa</em> leaves on testicular cumulative reproductive function biomarkers in streptozotocin (STZ)-induced diabetic rats.</div></div><div><h3>Methods</h3><div>Rats were induced with 40 mg/kg body weight (bwt) STZ intraperitoneally (i.p.), after which they were administered either a low (150 mg/kg bwt) or a high (300 mg/kg bwt) dose of flavonoid-rich extracts from <em>H. sabdariffa</em> leaves. Testicular redox biomarkers, reproductive hormone biomarkers, and relative gene expression levels of PDE-5 and iNOS were assessed. Additionally, histological data, glucose levels, G6PDH activity, and hydroxysteroid dehydrogenase activities (3β-HSD and 17β-HSD) were analyzed.</div></div><div><h3>Results</h3><div><em>H. sabdariffa</em> leaf extract was promising for restoring redox balance, normalizing reproductive hormone levels, modulating gene expression, and regulating various biochemical parameters related to testicular function.</div></div><div><h3>Conclusion</h3><div>These data suggest a potential therapeutic role of <em>H. sabdariffa</em> leaves in mitigating the testicular dysfunction associated with diabetes.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100808"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of curcumin in cadmium-induced physiological and reproductive toxicity in male BALB/c mice","authors":"Ranjit Shaw ,&nbsp;Raktim Hati ,&nbsp;Sandhya Sharma,&nbsp;Shikhar Deep,&nbsp;Radha Chaube","doi":"10.1016/j.phyplu.2025.100803","DOIUrl":"10.1016/j.phyplu.2025.100803","url":null,"abstract":"<div><div>Cadmium is one of the main worries these days due to its various harmful health effects, including adverse reproductive health concerns on humans. To overcome the side effects of synthetic drugs, we aimed to evaluate curcumin’s preventive role against cadmium-induced toxicity using an <em>in vivo</em> animal model. Treatment groups included control, cadmium chloride (6 mg/kg b.w.), curcumin (20 mg/kg b.w.), and cadmium chloride + curcumin (6 mg/kg and 20 mg/kg b.w., respectively), and the dosage was continued for 21 days. Our result demonstrated that curcumin ameliorates organs’ weight and sperm morphologies and improves the viability and fertility of sperm. Curcumin treatment led to a significant increase in catalase and SOD levels in the testis, liver, and kidney. Testosterone levels also got elevated; however, levels of Estradiol-17β decreased on treatment with curcumin. Moreover, the treatment restored vital genes such as Cyp19A1 and 3β-HSD, which were downregulated due to cadmium toxicity. Histological alterations due to cadmium toxicity in the testis, epididymis, and liver were restored with curcumin treatment. Results of H/E staining of the testis exhibited that the vacuolization of germinal epithelium due to cadmium toxicity improved due to curcumin treatment. Also, the concentration of the spermatogonia and spermatids appeared normal, and the seminiferous epithelium became well-organized. Histology of the liver showed that hepatocytes recovered in contrast to the cadmium-treated animals, and no infiltration of inflammatory cells was observed. However, future research needs to be envisioned to improve the oral bioavailability of curcumin, as it could act as a prospective candidate for the treatment of cadmium toxicity.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100803"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mistletoe (Viscum album L.) for human papillomavirus: A seven-case series","authors":"Nilo E. Gardin ,&nbsp;Beatriz Gardin-Machado","doi":"10.1016/j.phyplu.2025.100805","DOIUrl":"10.1016/j.phyplu.2025.100805","url":null,"abstract":"<div><h3>Background</h3><div>Human papillomavirus (HPV) is a significant public health concern, as 50 % of the population are at risk of being infected by this virus at least once in their lifetime. Although the host immune system clears most infections, a small percentage of patients do not fully clear genital HPV. They have an increased risk of developing HPV-associated cancers and can transmit HPV. Currently, no conventional treatments are available to eliminate the virus in HPV carriers.</div></div><div><h3>Cases presentation</h3><div>This study describes the clinical evolution of seven consecutive outpatients with HPV treated with <em>Viscum album</em> extract (VAE). In four patients, the persistence of HPV infection was six months or longer (in two of these patients, it had persisted for 22 months); in three patients, the diagnosis was recent. The patients were treated with topical (vaginal) VAE. For those with high-risk HPV or cervical intraepithelial neoplasia, subcutaneous injections of VAE were administered. <em>Calendula officinalis</em> (vaginal ovules) was prescribed for genital inflammation, and <em>Thuja occidentalis</em> ointment for verrucous lesions. Clinical and laboratory parameters were monitored throughout the treatment. All medications were well tolerated, with only mild skin reactions reported at the VAE injection site. Following treatment, six out of seven patients had negative hybrid capture tests for HPV. One of these six patients required a repeat course of treatment, after which the HPV hybrid capture test turned negative. In one patient, treatment failed to eradicate HPV.</div></div><div><h3>Conclusions</h3><div>Although this case series has a small sample size, there is a therapeutic potential to be further investigated regarding VAE for genital HPV. The treatment needs to be validated by future studies, which should be followed by a clinical trial to rigorously assess the efficacy of mistletoe for genital HPV infection.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100805"},"PeriodicalIF":0.0,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and pharmacological profiling of Balanites aegyptiaca linn.: Exploring the therapeutic potential of a traditional medicinal plant","authors":"Vikram Thakkar ,&nbsp;Prashant Kumar Dhakad ,&nbsp;Raghav Mishra ,&nbsp;Ritu M Gilhotra","doi":"10.1016/j.phyplu.2025.100804","DOIUrl":"10.1016/j.phyplu.2025.100804","url":null,"abstract":"<div><div>Numerous tree species that are underutilized can be used as food, fodder, and potentially medicinal substances. <em>Balanites aegyptiaca</em> (L.) Del. (<em>B. aegyptiaca</em>) (family Zygophyllaceae) is an evergreen xerophyte with ample beneficial attribute. It is effective to cure several diseases include malaria, epilepsy, constipation, intestinal worm infection, syphilis, asthma, jaundice, haemorrhoid, stomach aches, asthma, diarrhoea, dysentery, and wound healings. It also has contraceptive antifeedant, anti-carcinogenic, molluscicidal, antimicrobial, anti-inflammatory, anti-helminthic, antioxidant, immune-stimulant, antioxidant, antiviral and antidiabetic activities. <em>Balanites aegyptiaca</em> (L.) Del. (<em>B. aegyptiaca</em>) possesses various active compounds like lipids, carbohydrates, proteins, organic acids, alkaloids, saponins and flavonoids that mainly responsible for its pharmaceutical activities. In conclusion, the diverse pharmacological activities of this plant make it a valuable resource for developing new herbal remedies and pharmaceutical products. This review reveals bioactive compounds, medicinal uses, pharmacological significance, and toxicological effects of various parts of <em>Balanites aegyptiaca.</em></div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100804"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of Matrine in blood and prostate tissue: a microdialysis-HPLC study in a rabbit model","authors":"Yu Liu ,&nbsp;Gefei He ,&nbsp;Jianye Yan ,&nbsp;Meimin Pan ,&nbsp;Binqing Qu","doi":"10.1016/j.phyplu.2025.100801","DOIUrl":"10.1016/j.phyplu.2025.100801","url":null,"abstract":"<div><div>KangFuXiaoYan suppository exhibits therapeutic potential for chronic prostatitis (CP) ,however, its in vivo pharmacokinetic process remains to be elucidated. This study investigated the pharmacokinetics of Matrine, the primary bioactive component of KangFuXiaoYan suppository, using high-performance liquid chromatography (HPLC) with microdialysis in rabbits. The results showed that the C_max of Matrine in jugular vein and prostate tissue were (86.92 ± 2.66) and (45.33 ± 3.65) mg L^-1,the T_max were (30 ± 0.00) and (45 ± 0.00) min, the T_1/2 were (23.55 ± 1.76) and (26.50 ± 5.57) min, the AUC_0-t were (4192.77 ± 67.85) and (2919.78 ± 40.17) mg min L^-1,the MRT_0-t were (48.90 ± 0.13)and (56.25 ± 1 .06) min. Matrine absorption, distribution, and penetration into prostate tissue were quantified. The results demonstrated rapid absorption into blood and prostate tissue, achieving a penetration rate of 73 %, suggesting potential therapeutic effectiveness for chronic prostatitis treatment. These findings provided experimental support for the clinical application of rectally administered KangFuXiaoYan suppository.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100801"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the socio-cultural practices of medicinal plants used by the ethnic community of Soliga tribes in Tamil Nadu, India","authors":"Chinnasamy Menaka,&nbsp;Annadurai Logamadevi,&nbsp;Arumugam Vignesh","doi":"10.1016/j.phyplu.2025.100799","DOIUrl":"10.1016/j.phyplu.2025.100799","url":null,"abstract":"<div><h3>Background and purpose</h3><div>Plants have been integral to human health and cultural traditions for millennia, representing a vital relationship between tribal communities and their natural environment. The Soliga tribes have developed a profound relationship with the flora of their natural environment, utilizing various plant species in their socio-cultural practices. These practices are closely connected with their traditional knowledge, spirituality, and everyday life. This study aimed to document the socio-cultural practices associated with plant species and to identify the most culturally significant species used by the Soliga tribes in Anthiyur Taluk, Erode District, Tamil Nadu, India.</div></div><div><h3>Methods</h3><div>Ethnobotanical insights were collected through semi-structured interviews, group discussions, questionnaires, and field observations with knowledge holders. The collected data were analyzed using basic variables and ethnobotanical indices, whereas relationships and patterns within the dataset were explored through Pearson correlation and Principal component analysis (PCA).</div></div><div><h3>Results</h3><div>A total of 47 plant species from 25 families and 42 genera were identified as central to socio-cultural practices. Among these, Fabaceae, Apocynaceae, and Lamiaceae emerged as the predominant families. <em>Achyranthes aspera</em> L. was found to have the highest cultural importance index (CI) value of 0.98, followed by <em>Cassia fistula</em> L. and <em>Lantana camara</em> L. with CI values of 0.96 each. Ethnobotanical indices such as cultural importance index (CI), relative frequency of citation (RFC), relative importance index (RI) and cultural value index (CV) were applied, revealing significant positive correlations between basic variables and indices, such as CI and use reports (UR) (r = 0.82). Principal Component Analysis (PCA) further demonstrated that variables such as CI and RFC accounted for 60.24 % of the variance in the data.</div></div><div><h3>Conclusions</h3><div>The findings underscore the deep interconnection between Soliga tribes’ traditional knowledge and plant biodiversity, highlighting the urgency of preserving these cultural and ecological resources for future generations.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100799"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hesperidin protects against spermatological damages and testicular apoptosis induced by Bisphenol-A in adult male Wistar rats","authors":"Ezinne Chidinma Oviosun ,&nbsp;Augustine Oviosun ,&nbsp;Nto Johnson Nto ,&nbsp;Emeka Godson Anyanwu","doi":"10.1016/j.phyplu.2025.100797","DOIUrl":"10.1016/j.phyplu.2025.100797","url":null,"abstract":"<div><div>Bisphenol-A (BPA) is a prevalent environmental toxin affecting male fertility. Hesperidin (HSD) is a natural compound widely used as an antioxidant to combat damage induced by various environmental toxins. Hesperidin has remarkable pharmacological potential, which includes antioxidant, anti-inflammatory, anti-carcinogenic, and anti-apoptotic qualities. This study evaluated the protective effects of hesperidin on Bisphenol A-induced spermatological damage and testicular apoptosis in male rats. Thirty-five male Wistar rats were randomly divided into five equal-number groups. The control group orally received saline (1 mL); group B received Bisphenol A at 50 mg/kg/body weight orally, group C was administered with 200 mg/kg of Hesperidin only. Groups D and E orally received hesperidin at 100 and 200 mg/kg for 28 days, respectively, followed by treatment with 50 mg/kg Bisphenol-A for 28 days. The parameters, including sperm characteristics (sperm morphology, motility, and count), were measured, and the immunohistochemical testicular expression of Bcl-2-associated X protein (BAX) and B-cell lymphoma-2 (Bcl2) was determined. BPA significantly (<em>P</em> &lt; 0.05) reduced normal morphology, progressive motility, and sperm count. High immunopositive cell expression of BAX immunoprotein and low immunopositive cell expression of Bcl2 immunoprotein were also observed in the BPA-exposed group. Hesperidin pretreatment before exposure to Bisphenol-A increased progressive motility, sperm count, and normal morphology and reduced Bisphenol A-mediated apoptosis by downregulating BAX expression, whereas testicular Bcl-2 immunopositive cell expression levels increased. Our findings suggest that hesperidin may protect against spermatological damages and apoptosis induced by Bisphenol-A in adult male Wistar rats.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100797"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing nature's potential: Exploring phytoestrogens in cervical cancer","authors":"Alaa A.A. Aljabali ,&nbsp;Rasha M. Bashatwah ,&nbsp;Omar Gammoh ,&nbsp;Esam Qnais ,&nbsp;Abdelrahim Alqudah ,&nbsp;Vijay Mishra ,&nbsp;Yachana Mishra ,&nbsp;Mohamed El-Tanani","doi":"10.1016/j.phyplu.2025.100796","DOIUrl":"10.1016/j.phyplu.2025.100796","url":null,"abstract":"<div><div>Phytoestrogens are plant-derived compounds with structural similarity to 17-β-estradiol and have attracted increasing research interest due to their estrogenic and antiestrogenic properties. This review provides a comprehensive overview of the types, metabolism, and bioavailability of major phytoestrogens, including isoflavones and lignans, and their potential role in cervical cancer prevention. Isoflavones and lignans found primarily in soy-based foods and are more abundantly in flaxseeds and whole grains, respectively are the two main phytoestrogen classess. The metabolism of these compounds is complex, influenced by gut microbiota and genetic factors, and leads to the production of bioactive metabolites such as equol and enterolactone, respectively. These metabolites interact with estrogen receptor pathways, producing antioxidant, anti-inflammatory, and pro-apoptotic effects, which supports their role modulating hormone-sensitive conditions. Some epidemiological data suggest a possible inverse association between phytoestrogen intake and cervical cancer risk, although findings remian inconsistent due to variations in dietary assessment methods and inter-individual metabolic responses. Molecular and experimental studies show that phytoestrogens inhibit cell proliferation, promote apoptosis in cervical cancer cells, and reduce oxidative stress, aligning with their proposed preventive and therapeutic benefits. This review also outlines several methodological challenges in phytoestrogen research, including the lack of standardized measurement techniques, variability in the dietery intake assessment, and the influence of genetic and ethnic differences on metabolism and bioavailability. These factors highlight the need for interdisciplinary research to improe precision and comparability. The implications for female health are significant. Phytoestrogens represent a cost-effective, dietary-based strategy that may complement existing interventions—such as HPV vaccination and cervical screening—to improve cervical health outcomes. Emerging technologies, including metabolomics and genomic profiling, may support the development of personalized dietary recommendations and expand understanding of the therapeutic potential of phytoestrogens. The synthesis of current evidence underscores the importance of phytoestrogens in preventive medicine and suggests new directions for advancing women's health and cervical cancer management.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100796"},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential therapeutic effects of interleukin-1 receptor type 1 antagonist and the ethyl acetate fraction of Murraya exotica leaves against κ-carrageenan induced vein thrombosis in rats","authors":"Asmaa F. Aboul Naser ,&nbsp;Wessam M. Aziz ,&nbsp;Amal M. El-Feky ,&nbsp;Marwa M. Elbatanony ,&nbsp;Noha N. Nasr ,&nbsp;Yomna R. Ahmed ,&nbsp;Sylvia E. Shaker ,&nbsp;Wagdy K.B. Khalil ,&nbsp;Manal A. Hamed","doi":"10.1016/j.phyplu.2025.100795","DOIUrl":"10.1016/j.phyplu.2025.100795","url":null,"abstract":"<div><h3>Background</h3><div>Thrombosis is the primary cause of cardiovascular diseases, cerebral ischemia, hypoxia, tissue softening, and necrosis. One of the best models used to assess antithrombotic and thrombolytic drugs is the κ-carrageenan induced vein tail thrombosis rats model.</div></div><div><h3>Purpose</h3><div>The purpose of this study is to evaluate the treatment of vein thrombosis by blocking intrelukin-1 receptor type 1 (IL-1R1) <em>via</em> its antagonist (IL-1RA1) comparing with the treatment role of the ethyl acetate fraction (EAF) of <em>Murraya exotica</em> L. leaves.</div></div><div><h3>Study design</h3><div>The major bioactive compounds from the EAF were identified by HPLC and LC-ESI-MS/MS analyses, while, isolation achieved <em>via</em> UV, IR, MS, ¹HNMR , and ¹³CNMR . After 24 h of κ-carrageenan induction to rats, treatments occurred and the evaluation was done through measuring hemagglutination index (thrombin time (TT), prothrombin time (PT), and fibrinogen), genes expression of IL-1R1, interleukin-1 beta (IL-1β), vascular cell adhesion molecule-1 (VCAM-1), malondialdehyde (MDH), glutathione (GSH) and superoxide dismutase (SOD), aspartate and alanine aminotransferase (AST&amp; ALT), alkaline phosphatase (ALP), total protein, urea, creatinine, kidney injury molecule-1 (KIM-1) and the histopathological analysis of tail tissues.</div></div><div><h3>Results</h3><div>The HPLC analysis led to characterization of 11 phenolic acids and 7 flavonoids. LC-ESI-MS data detected 62 compounds while, 4 compounds were isolated: Cirsiliol 4′-glucoside (1), p-coumaric acid (2), bismurrangatin (3) and 5, 7-dihydroxy-3,6-dimethoxy-3′,4′-methylenedioxyflavone (4). The results revealed that treatment with IL-1RA1 and the EAF of <em>M. exotica</em> leaves manifested anti-thrombosis effects by alleviating all the biochemical parameters under investigation.</div></div><div><h3>Conclusion</h3><div>IL-1RA1 and the EAF could be considered as antithrombotic, thrombolytic and anti-inflammatory agents that may be served as good candidates for disease treatment.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100795"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}