Phytomedicine Plus最新文献

{"title":"Unveiling the socio-cultural practices of medicinal plants used by the ethnic community of Soliga tribes in Tamil Nadu, India","authors":"Chinnasamy Menaka,&nbsp;Annadurai Logamadevi,&nbsp;Arumugam Vignesh","doi":"10.1016/j.phyplu.2025.100799","DOIUrl":"10.1016/j.phyplu.2025.100799","url":null,"abstract":"<div><h3>Background and purpose</h3><div>Plants have been integral to human health and cultural traditions for millennia, representing a vital relationship between tribal communities and their natural environment. The Soliga tribes have developed a profound relationship with the flora of their natural environment, utilizing various plant species in their socio-cultural practices. These practices are closely connected with their traditional knowledge, spirituality, and everyday life. This study aimed to document the socio-cultural practices associated with plant species and to identify the most culturally significant species used by the Soliga tribes in Anthiyur Taluk, Erode District, Tamil Nadu, India.</div></div><div><h3>Methods</h3><div>Ethnobotanical insights were collected through semi-structured interviews, group discussions, questionnaires, and field observations with knowledge holders. The collected data were analyzed using basic variables and ethnobotanical indices, whereas relationships and patterns within the dataset were explored through Pearson correlation and Principal component analysis (PCA).</div></div><div><h3>Results</h3><div>A total of 47 plant species from 25 families and 42 genera were identified as central to socio-cultural practices. Among these, Fabaceae, Apocynaceae, and Lamiaceae emerged as the predominant families. <em>Achyranthes aspera</em> L. was found to have the highest cultural importance index (CI) value of 0.98, followed by <em>Cassia fistula</em> L. and <em>Lantana camara</em> L. with CI values of 0.96 each. Ethnobotanical indices such as cultural importance index (CI), relative frequency of citation (RFC), relative importance index (RI) and cultural value index (CV) were applied, revealing significant positive correlations between basic variables and indices, such as CI and use reports (UR) (r = 0.82). Principal Component Analysis (PCA) further demonstrated that variables such as CI and RFC accounted for 60.24 % of the variance in the data.</div></div><div><h3>Conclusions</h3><div>The findings underscore the deep interconnection between Soliga tribes’ traditional knowledge and plant biodiversity, highlighting the urgency of preserving these cultural and ecological resources for future generations.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100799"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing nature's potential: Exploring phytoestrogens in cervical cancer","authors":"Alaa A.A. Aljabali ,&nbsp;Rasha M. Bashatwah ,&nbsp;Omar Gammoh ,&nbsp;Esam Qnais ,&nbsp;Abdelrahim Alqudah ,&nbsp;Vijay Mishra ,&nbsp;Yachana Mishra ,&nbsp;Mohamed El-Tanani","doi":"10.1016/j.phyplu.2025.100796","DOIUrl":"10.1016/j.phyplu.2025.100796","url":null,"abstract":"<div><div>Phytoestrogens are plant-derived compounds with structural similarity to 17-β-estradiol and have attracted increasing research interest due to their estrogenic and antiestrogenic properties. This review provides a comprehensive overview of the types, metabolism, and bioavailability of major phytoestrogens, including isoflavones and lignans, and their potential role in cervical cancer prevention. Isoflavones and lignans found primarily in soy-based foods and are more abundantly in flaxseeds and whole grains, respectively are the two main phytoestrogen classess. The metabolism of these compounds is complex, influenced by gut microbiota and genetic factors, and leads to the production of bioactive metabolites such as equol and enterolactone, respectively. These metabolites interact with estrogen receptor pathways, producing antioxidant, anti-inflammatory, and pro-apoptotic effects, which supports their role modulating hormone-sensitive conditions. Some epidemiological data suggest a possible inverse association between phytoestrogen intake and cervical cancer risk, although findings remian inconsistent due to variations in dietary assessment methods and inter-individual metabolic responses. Molecular and experimental studies show that phytoestrogens inhibit cell proliferation, promote apoptosis in cervical cancer cells, and reduce oxidative stress, aligning with their proposed preventive and therapeutic benefits. This review also outlines several methodological challenges in phytoestrogen research, including the lack of standardized measurement techniques, variability in the dietery intake assessment, and the influence of genetic and ethnic differences on metabolism and bioavailability. These factors highlight the need for interdisciplinary research to improe precision and comparability. The implications for female health are significant. Phytoestrogens represent a cost-effective, dietary-based strategy that may complement existing interventions—such as HPV vaccination and cervical screening—to improve cervical health outcomes. Emerging technologies, including metabolomics and genomic profiling, may support the development of personalized dietary recommendations and expand understanding of the therapeutic potential of phytoestrogens. The synthesis of current evidence underscores the importance of phytoestrogens in preventive medicine and suggests new directions for advancing women's health and cervical cancer management.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100796"},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential therapeutic effects of interleukin-1 receptor type 1 antagonist and the ethyl acetate fraction of Murraya exotica leaves against κ-carrageenan induced vein thrombosis in rats","authors":"Asmaa F. Aboul Naser ,&nbsp;Wessam M. Aziz ,&nbsp;Amal M. El-Feky ,&nbsp;Marwa M. Elbatanony ,&nbsp;Noha N. Nasr ,&nbsp;Yomna R. Ahmed ,&nbsp;Sylvia E. Shaker ,&nbsp;Wagdy K.B. Khalil ,&nbsp;Manal A. Hamed","doi":"10.1016/j.phyplu.2025.100795","DOIUrl":"10.1016/j.phyplu.2025.100795","url":null,"abstract":"<div><h3>Background</h3><div>Thrombosis is the primary cause of cardiovascular diseases, cerebral ischemia, hypoxia, tissue softening, and necrosis. One of the best models used to assess antithrombotic and thrombolytic drugs is the κ-carrageenan induced vein tail thrombosis rats model.</div></div><div><h3>Purpose</h3><div>The purpose of this study is to evaluate the treatment of vein thrombosis by blocking intrelukin-1 receptor type 1 (IL-1R1) <em>via</em> its antagonist (IL-1RA1) comparing with the treatment role of the ethyl acetate fraction (EAF) of <em>Murraya exotica</em> L. leaves.</div></div><div><h3>Study design</h3><div>The major bioactive compounds from the EAF were identified by HPLC and LC-ESI-MS/MS analyses, while, isolation achieved <em>via</em> UV, IR, MS, ¹HNMR , and ¹³CNMR . After 24 h of κ-carrageenan induction to rats, treatments occurred and the evaluation was done through measuring hemagglutination index (thrombin time (TT), prothrombin time (PT), and fibrinogen), genes expression of IL-1R1, interleukin-1 beta (IL-1β), vascular cell adhesion molecule-1 (VCAM-1), malondialdehyde (MDH), glutathione (GSH) and superoxide dismutase (SOD), aspartate and alanine aminotransferase (AST&amp; ALT), alkaline phosphatase (ALP), total protein, urea, creatinine, kidney injury molecule-1 (KIM-1) and the histopathological analysis of tail tissues.</div></div><div><h3>Results</h3><div>The HPLC analysis led to characterization of 11 phenolic acids and 7 flavonoids. LC-ESI-MS data detected 62 compounds while, 4 compounds were isolated: Cirsiliol 4′-glucoside (1), p-coumaric acid (2), bismurrangatin (3) and 5, 7-dihydroxy-3,6-dimethoxy-3′,4′-methylenedioxyflavone (4). The results revealed that treatment with IL-1RA1 and the EAF of <em>M. exotica</em> leaves manifested anti-thrombosis effects by alleviating all the biochemical parameters under investigation.</div></div><div><h3>Conclusion</h3><div>IL-1RA1 and the EAF could be considered as antithrombotic, thrombolytic and anti-inflammatory agents that may be served as good candidates for disease treatment.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100795"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological profiling of trypanocidal principles from Aloe barbadensis and Taraxacum officinale","authors":"Pearl Ihuoma Akazue ,&nbsp;Neils Ben Quashie ,&nbsp;Dorcas Osei-Safo ,&nbsp;Sue Vaughan ,&nbsp;Harry P. de Koning ,&nbsp;Theresa Manful Gwira","doi":"10.1016/j.phyplu.2025.100793","DOIUrl":"10.1016/j.phyplu.2025.100793","url":null,"abstract":"<div><div>The use of herbal medicines to treat ailments is a common practice in several regions in Africa, relying on knowledge systems that have evolved over several generations. These herbal remedies are often based on anecdotal claims, many of which lack scientific validation. This study investigates the mode of action of two bioactive fractions, F1 (IC₅₀: 8.5 µg/mL) and F5 (IC₅₀: 7.4 µg/mL), derived from a dichloromethane extract of a herbal mixture, consisting of <em>Aloe barbadensis</em> and <em>Taraxacum officinale</em>, that is commonly used in Ghana to treat parasitic fevers. Both fractions exhibited trypanocidal effects with minimal cytotoxicity to mammalian cells. F5 induced necrotic cell death through mitochondrial oxidative stress, evidenced by a 3.5-fold increase in mitochondrial reactive oxygen species at 2 × IC₅₀ (<em>p</em>&lt; 0.0001) and significant mitochondrial membrane depolarization (<em>p</em>&lt; 0.01). In contrast, F1 primarily disrupted kinetoplast segregation, increasing 2K1 N cells by 3.2-fold at 1 × IC₅₀ (<em>p</em>&lt; 0.0001) and instigating an accumulation of dyskinetoplastic cells (0KXN). Both fractions induced morphological distortions, nuclear fragmentation, and loss of flagellar integrity. This study provides the first mechanistic insights into the antitrypanosomal activity of bioactive fractions obtained from a mixture of <em>A. barbadensis</em> and <em>T. officinale</em>. The distinct targeting of mitochondrial ROS production (F5) and kinetoplast replication (F1) highlights their potential as leads for the development of new antitrypanosomal drugs with novel mechanisms of action. These findings reinforce the value of ethnomedicinal plants as sources of novel bioactive compounds.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100793"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANGOCIN Anti-Infekt N in the treatment of urinary and respiratory tract infections: A pharmacy-based non-interventional observational study","authors":"Elisabeth Gabor,&nbsp;Nina Kassner,&nbsp;Meinolf Wonnemann,&nbsp;Yvonne Ziegler","doi":"10.1016/j.phyplu.2025.100782","DOIUrl":"10.1016/j.phyplu.2025.100782","url":null,"abstract":"<div><h3>Background</h3><div>Several studies have verified the efficacy and safety of a nasturtium and horseradish combination (ANGOCIN <em>Anti-Infekt N,</em> further referenced as ANGOCIN) for the treatment of acute respiratory and urinary tract infections.</div></div><div><h3>Study design and purpose</h3><div>This was a prospective pharmacy-based non-interventional cohort study to gain further insights into the use of ANGOCIN to treat respiratory (RTI) and urinary tract infections (UTI) under real-world conditions using a web-based patient survey (electronic patient reported outcome (ePRO)).</div></div><div><h3>Methods</h3><div>Participants were asked to register and take part in a patient survey when they purchased ANGOCIN at a German pharmacy. They were asked to answer the survey questions over a 14-day period.</div></div><div><h3>Results</h3><div>Participants (N = 479, mean age 41.5 ± 13.8 years) reported significant improvement of symptoms and subjective well-being under ANGOCIN treatment. For most participants (&gt;60 %), the first symptom improvement occurred within the first two days of therapy, and the time to complete or sufficient symptom improvement was 12 days. This corresponds directly to the respective median duration of ANGOCIN intake (12 Days for RTI, 11 Days for UTI). Furthermore, almost all participants (96.5 %) were satisfied or even better with ANGOCIN treatment. Most of the participants (90.2 %) did not require additional antibiotics to treat their acute infection. The main reason for study discontinuation was the feeling of regaining health after ANGOCIN therapy (60 %); only a few participants terminated ANGOCIN therapy due to low tolerability (1.2 %). Most of the participants (96.5 %) stated that they intend to use ANGOCIN again for the next RTI or UTI, respectively.</div></div><div><h3>Conclusions</h3><div>These results are in line with results from other studies that herbal medications contribute to the recovery of infections. Furthermore, they reflect the S3-guideline statement that symptom-reducing herbal medications are a good alternative to antibiotics in treating uncomplicated acute and recurrent UTI. ANGOCIN is an effective and well-tolerated self-medication for RTI and UTI.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100782"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the reproductive potential of selected flavonoids in Biden pilosa: A comprehensive review","authors":"Ismahil Adekunle Adeniyi ,&nbsp;Onaadepo Olufunke ,&nbsp;Ibe Michael Usman ,&nbsp;Daniel Udofia Owu","doi":"10.1016/j.phyplu.2025.100784","DOIUrl":"10.1016/j.phyplu.2025.100784","url":null,"abstract":"<div><div><em>Bidens pilosa</em> is a medicinal plant that has gained lots of attention for its potential use in treating various health conditions including reproductive disorders. This narrative review assessed the effectiveness and safety of <em>Bidens pilosa</em> in addressing reproductive conditions. A review of relevant scientific literature was conducted; the review identified evidence for <em>Biden pilosa's</em> potential to improve sperm health in men and regulation of menstrual cycles in women. The flavonoids contained in <em>Bidens pilosa</em> include astragalene, squalene, luteolin, and quercetin; these flavonoids act as antioxidant by scavenging free radicals and reducing reactive oxygen species level to protect the integrity of cell membrane or spermatogenic cell membrane. They also exhibit anti-inflammatory effects by reducing the level of anti-inflammatory cytokines, such as interleukin-6, interleukin-1β, and tumor necrosis factor-α, through reduction of the expression of inflammatory genes such as Inducible nitric oxide synthase and cyclooxygenase-2. The reduction can prevent production of toxic nitric oxide, mitigating damage to testicular tissues or cells. These findings highlight the need for further investigation through well-structured preclinical and clinical trials to assess the efficacy and safety of <em>Bidens pilosa</em> in treating specific reproductive disorders.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100784"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antioxidant, anxiolytic and antidepressant potential of Saurauia roxburghii Wall. Leaves: Supported by in vitro, in vivo, and in silico approaches","authors":"Md. Asibur Rahman ,&nbsp;Kishore Kumar Sarkar ,&nbsp;Md. Aktaruzzaman ,&nbsp;Trina Mitra ,&nbsp;Md. Takim Sarker ,&nbsp;Md. Ahsan Abid ,&nbsp;Kishor Mazumder ,&nbsp;Apurba Kumar Barman ,&nbsp;Nazim Uddin Molla ,&nbsp;A.S.M. Monjur Al Hossain","doi":"10.1016/j.phyplu.2025.100792","DOIUrl":"10.1016/j.phyplu.2025.100792","url":null,"abstract":"<div><div><em>Saurauia roxburghii,</em> locally known as Dalup, has been utilizing as a treatment for Asthma, Bronchitis, Central Nervous System (CNS) Depression, Eczema, Fever, Gout, Hepatitis B, Piles, and Ulcers by inhabitants from hill-tract of Bangladesh for many years. Blended scientific approaches were applied to evaluate the antioxidant and neuropharmacological activities of the methanol extract of <em>Saurauia roxburghii</em> Wall. Leaves (MSRL) followed by experimental and <em>in silico</em> analysis. Firstly, the qualitative phytochemical screening, acute toxicity study and antioxidant activity assays were conducted. Then elevated plus maze, hole board, hole cross, open field, forced swimming, and tail suspension tests for neuropharmacological potentials were also investigated. Finally, molecular docking and ADMET analysis were carried out based on the bioactive compounds identified by GC-MS profiling. MSRL exhibited moderate antioxidant activity with a substantial amount of antioxidant components in different <em>in vitro</em> assays. In the Elevated Plus Maze and Hole Board test, a higher dose of MSRL (400 mg/kg) exhibited a significant reduction in anxiety-like behavior evidenced by enhanced time spent and entries into open arms, and an increase in the number of head dips, respectively suggesting anxiolytic activity. Moreover, dose-dependent locomotor activity was observed by MSRL in both Open Field and Hole Cross tests. In terms of Forced Swimming and Tail Suspension tests, both doses (200 and 400 mg/kg) of MSRL significantly declined the immobile time of mice indicating antidepressant effects. Lastly, in molecular docking analysis, amongst the identified compounds in GC-MS analysis, 5-(piperidin-1-ylmethyl)-3-pyridin-3-yl-5,6-dihydro-4h-1,2,4-oxadiazine showed strongest binding affinity against all the targeted receptors (Potassium channels receptor, GABA_A receptor, and human serotonin transporter) followed by satisfactory ADMET profiling. So, <em>S. roxburghii</em> might have the potential to be used as a prominent source of novel therapeutics for anxiety and depression justifying the traditional usage.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100792"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, anti-glycation and hypoglycemic potentials of Acmella uliginosa: A probable candidate for the amelioration of diabetes mellitus","authors":"Mahfuza Akter,&nbsp;Faria Farzana Perveen,&nbsp;Noore Zannat Nuren,&nbsp;Nasim Ahmed,&nbsp;Md. Ataur Rahman,&nbsp;Md. Masum Billah,&nbsp;Md. Abdul Bari,&nbsp;Sabbir Ahmed,&nbsp;Md. Nurul Islam","doi":"10.1016/j.phyplu.2025.100777","DOIUrl":"10.1016/j.phyplu.2025.100777","url":null,"abstract":"<div><h3>Objective</h3><div><em>Acmella uliginosa</em>, widely distributed across Asia, has long been used traditionally for various purposes. This study explored the pharmacological potential of its whole plant and flower extracts, focusing on antioxidant, anti-glycation, and <em>in vivo</em> anti-diabetic activities.</div></div><div><h3>Materials and methods</h3><div>The methanolic extracts of <em>A. uliginosa</em> whole plants and flowers were analyzed for flavonoid, phenolic content, and total antioxidant capacity, expressed as gallic acid, rutin, and ascorbic acid equivalents, respectively. Antioxidant activity was assessed using DPPH and ABTS assays. The inhibitory effect on AGE formation was evaluated by fluorometric analysis, along with protein carbonyl content and thiol group modifications in glycated BSA. The antidiabetic potential of the extracts was tested through the OGTT, and hypoglycemic activity both in normal mice, and alloxan-induced diabetic mice.</div></div><div><h3>Results</h3><div>In antiglycation studies, <em>A. uliginosa</em> flower extract showed superior inhibition of AGEs formation compared to the whole plant extract, and its effect at 4 mg/mL was comparable to aminoguanidine. The flower extract also effectively reduced protein carbonyl content and preserved thiol group levels in a dose-dependent manner, indicating its strong protective effect against protein damage caused by oxidative stress. It exhibited higher flavonoid content and stronger antioxidant activity than the whole plant extract. <em>In vivo</em> studies confirmed the antidiabetic potential of both extracts, with significant reductions in blood glucose levels in OGTT and hypoglycemic tests. After seven days, the flower extract lowered blood glucose in diabetic mice to near-normal levels, similar to glibenclamide.</div></div><div><h3>Conclusion</h3><div>Therefore, the plant <em>A. uliginosa</em> might be a promising herbal medicine to prevent diabetes and its associated complications by inhibiting AGEs formation, lowering blood glucose level, and reducing oxidative damage of proteins.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100777"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess strategies for maximizing SCOBY growth and evaluating fermentation dynamics on phenolic content and antioxidant activity in Roselle-based Kombucha","authors":"Widiastuti Setyaningsih ,&nbsp;Wa Ode Reza Sindy Warni ,&nbsp;Intan Dewi Larasati ,&nbsp;Rini Yanti ,&nbsp;Tyas Utami","doi":"10.1016/j.phyplu.2025.100791","DOIUrl":"10.1016/j.phyplu.2025.100791","url":null,"abstract":"<div><div>Roselle-based kombucha is a promising functional beverage due to its bioactive compounds, high phenolic content, and anthocyanins, which contribute to color stability, anti-inflammatory effects, and cardioprotective benefits. This study aimed to optimize fermentation parameters—roselle concentration, sucrose levels, and initial symbiotic culture of bacteria and yeast (SCOBY) mass—to enhance microbial activity, phenolic stability, and antioxidant properties.</div><div>High-performance liquid chromatography (HPLC) identified chlorogenic acid (270.62 mg L⁻¹), quercetin-3-glucoside (31.96 mg L⁻¹), and rutin (7.7 mg L⁻¹) as predominant phenolics, with protocatechuic acid increasing over 14 days. Optimized fermentation conditions (5 g roselle, 12% sucrose, 25 g SCOBY) resulted in higher SCOBY growth (41.66 g) and superior bioactive stability compared to traditional tea kombucha. Fermentation significantly influenced key parameters: pH remained stable (2.16 to 2.21) due to buffering organic acids, titratable acidity increased (0.60% to 2.33%), and sucrose concentration decreased (12.0 to 10.2 °Brix). Color analysis (CIELab) revealed dynamic pigment changes, reflecting anthocyanin degradation and transformation. Storage stability tests showed that phenolic compounds remained stable at 4°C, while room-temperature storage increased phenolic content, likely due to continued microbial metabolism. Antioxidant activity assays confirmed robust antioxidant potential, with IC50 values remaining stable in cold storage: DPPH (9.71 to 9.59 mg L⁻¹), ABTS (8.68 to 7.60 mg L⁻¹), and FRAP (10.01 to 9.84 mg L⁻¹). These findings underscore roselle kombucha's potential as a scalable, nutritionally enhanced functional beverage, emphasizing its phenolic stability and antioxidant properties under optimized fermentation and storage conditions. This study provides practical insights for scaling production, improving cost-efficiency, and advancing functional beverage development.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100791"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anxiety behavior in adult Zebrafish modulated by the alkaloid fraction of Cissampelos sympodialis Eichl. roots","authors":"Johnatan Wellisson da Silva Mendes ,&nbsp;Joice Barbosa do Nascimento ,&nbsp;José Jonas Ferreira Viturino ,&nbsp;Lariza Leisla Leandro Nascimento ,&nbsp;Débora Odília Duarte Leite ,&nbsp;Natália Kelly Gomes de Carvalho ,&nbsp;Hélcio Silva dos Santos ,&nbsp;Emmanuel Silva Marinho ,&nbsp;Márcia Machado Marinho ,&nbsp;Jane Eire Silva Alencar de Menezes ,&nbsp;José Galberto Martins da Costa","doi":"10.1016/j.phyplu.2025.100789","DOIUrl":"10.1016/j.phyplu.2025.100789","url":null,"abstract":"<div><div><em>Cissampelos sympodialis</em> (Menispermaceae), a species endemic to Brazil, it is used in folk medicine to treat inflammation and respiratory disorders. These activities are probably associated with the presence of alkaloids in the plant. This study aims to investigate the effect of the alkaloid fraction of <em>C. sympodialis</em> (FARCs) on anxiety behavior in adult zebrafish. Locomotor activity was evaluated using the open field test and acute toxicity was analyzed within 96 h. The light and dark tests were applied for anxiolytic evaluation. Berberine (BRB) quantification was performed in FARCs, through spectrophotometry. GABAergic neuromodulation was evaluated, and molecular docking was performed. FARCs was not toxic to adult zebrafish, all doses indicated a sedative effect, also caused anxiolytic behavior in animals. The anxiety mechanism via GABAergic neurotransmission was confirmed. It was observed that the BRB corresponds to the majority of the composition of the FARCs. Molecular docking showed that BRB can exert neuromodulatory function on GABA receptors with safety and metabolic stability. The study indicated that FARCs exhibit an anxiolytic effect on animals without showing toxicity. Quantitative analysis determined for the first time in the species the presence of BRB. Molecular docking indicated that berberine constitutes a promising active principle in neuromodulation.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100789"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}