Phytomedicine Plus最新文献

{"title":"Antispasmodic and antidiarrheal activity of cannabidiol (CBD): involvement of K⁺ channels in intestinal smooth muscle","authors":"Cristian Vaccarini ,&nbsp;Alicia E. Consolini ,&nbsp;Darío Andrinolo ,&nbsp;Daniela Sedan","doi":"10.1016/j.phyplu.2025.100922","DOIUrl":"10.1016/j.phyplu.2025.100922","url":null,"abstract":"<div><h3>Background</h3><div>Functional gastrointestinal disorders, such as irritable bowel syndrome and colitis, are highly prevalent and often poorly responsive to conventional therapies. Cannabidiol (CBD), a non-psychoactive phytocannabinoid, has been reported to modulate intestinal motility. Purpose: To evaluate the antispasmodic and antidiarrheal effects of CBD and investigate the mechanisms underlying its action. Study design: A combined <em>in vitro</em> and <em>in vivo</em> experimental approach was used to assess spasmolytic and antidiarrheal properties. Methods: Antispasmodic activity was evaluated in isolated rat small intestine using carbachol-induced concentration–response curves (CCh-CRCs), with CBD applied at 30–300 ng/mL. CBD solution employed was quantified by HPLC/UV-DAD. Mechanistic studies included calcium-induced CRCs and potassium channel blockade with tetraethylammonium (TEA, 20 mM) or glibenclamide (GLIB, 50 μM). <em>In vivo</em> antidiarrheal activity was tested in a murine model of castor oil-induced diarrhea, with oral CBD at 3, 10, and 30 mg/kg. Results: CBD produced a non-competitive inhibition of CCh-induced contractions (IC₅₀ = 0.154 ± 0.054 μg/mL). Its affinity constant (pKCBD) was 6.97, decreasing to 5.87 and 5.93 in the presence of TEA and GLIB, respectively. CBD did not affect calcium-induced contractions, suggesting a mechanism independent of <span>l</span>-type Ca²⁺ channels<em>. In vivo</em>, at 10 and 30 mg/kg, CBD significantly increased dried feces number and provided 56 % protection, comparable to loperamide, without reducing total defecation. Conclusion: CBD exerts antispasmodic and antidiarrheal effects mediated by potassium channels, with evidence indicating a possible contribution of K_ATP channels. These findings support its therapeutic potential in functional gastrointestinal disorders.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100922"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deltan polyherbal formula offers multifaceted protection against cardiometabolic syndrome via metabolic and microbiome rebalancing","authors":"Vito Anggarino Damay ,&nbsp;Juan Leonardo ,&nbsp;Axel Brahmantyo Maynardo Nugroho ,&nbsp;Vincent Lau ,&nbsp;Arditya Damarkusuma ,&nbsp;Dante Saksono Harbuwono ,&nbsp;Sidartawan Soegondo ,&nbsp;Happy Kurnia Permatasari ,&nbsp;Ratna Karuna ,&nbsp;Nurpudji Astuti Taslim ,&nbsp;Raymond Rubianto Tjandrawinata ,&nbsp;Hilman Zulkifli Amin ,&nbsp;Antonello Santini ,&nbsp;Sergio Vencio ,&nbsp;Rony Abdi Syahputra ,&nbsp;Fahrul Nurkolis","doi":"10.1016/j.phyplu.2025.100929","DOIUrl":"10.1016/j.phyplu.2025.100929","url":null,"abstract":"<div><h3>Objective</h3><div>Cardiometabolic syndrome (CMS) increases risks for type 2 diabetes and cardiovascular disease. Although individual herbal extracts such as <em>Panax ginseng, Panax notoginseng</em>, and <em>Salvia miltiorrhiza</em> have demonstrated metabolic and cardiovascular benefits, their combined synergistic actions have not been comprehensively evaluated within an integrated preclinical framework. This study aimed to evaluate the efficacy of Deltan, a polyherbal formulation of <em>Panax ginseng, Panax notoginseng</em>, and <em>Salvia miltiorrhiza</em>, in mitigating CMS by modulating oxidative stress, lipid metabolism, inflammation, and gut microbiota.</div></div><div><h3>Methods</h3><div>A multi-tiered approach was employed, including UHPLC–HRMS metabolomic profiling, in silico molecular docking, in vitro antioxidant, lipase-inhibitory, and cardiomyoblast viability assays, and an eight-week randomized, controlled preclinical trial in Wistar rats fed a cholesterol- and fat-enriched diet. Evaluations included biochemical parameters, inflammatory mediators, and gut microbiota analysis via 16S rRNA sequencing.</div></div><div><h3>Results</h3><div>Deltan demonstrated potent antioxidant (IC₅₀ = 45.2 ± 2.3 µg/mL for DPPH scavenging) and lipase-inhibitory activities (IC₅₀ = 38.7 ± 1.9 µg/mL), effectively preserving H9c2 cardiomyoblast viability (89.6 ± 3.4 % at 100 µg/mL) and reducing PCSK9 (↓ 31 %) and Galectin-3 (↓ 27 %) secretion, comparable to simvastatin (↓ 35 % and ↓ 29 %, respectively). In vivo, Deltan prevented diet-induced weight gain (−18.4 % vs. CFED control), normalized serum lipids (total cholesterol ↓ 29 %, triglycerides ↓ 24 %, HDL ↑ 18 %) and glucose (↓ 22 %), attenuated hepatic stress markers (ALT and AST ↓ ∼30 %), and regulated inflammatory and lipid-regulatory biomarkers including TNF-α (↓ 33 %), PCSK9 (↓ 36 %), ANGPTL3 (↓ 28 %), and PLA2G7 (↓ 25 %).</div></div><div><h3>Conclusion</h3><div>Deltan effectively targets multiple aspects of CMS through antioxidative, anti-inflammatory, lipid-regulatory, and microbiota-modulating mechanisms. These preclinical findings highlight its potential as a safe, multi-target phytotherapeutic intervention for CMS. Future studies should isolate active phytochemicals, elucidate detailed molecular pathways, and validate efficacy through clinical trials.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100929"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the phytochemical and pharmacological potential of Ocimum sanctum L. and Plectranthus amboinicus (Lour.) Spreng. toward developing novel evidence-based herbal products in Sri Lanka: A comprehensive review","authors":"Isuru Sakbo Uyangoda ,&nbsp;Mayuri Munasinghe","doi":"10.1016/j.phyplu.2025.100920","DOIUrl":"10.1016/j.phyplu.2025.100920","url":null,"abstract":"<div><div><em>Ocimum sanctum</em> L. (Heen Maduruthala) and <em>Plectranthus amboinicus</em> (Lour.) Spreng. (Kapparawalliya) are two highly abundant medicinal plants belonging to the family Lamiaceae. Despite their widespread availability and documented ethnomedicinal uses, their phytopharmacological potential remains underexplored in Sri Lanka. Consequently, their utilization of herbal products remains limited. The present review investigates the scientific evidence on phytochemical constituents, pharmacological potential, and safety data of those species and evaluates the research gap regarding their potential for novel herbal product development in Sri Lanka. The scientific data were collected via PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar databases on both plants from the past 30 years, and they were critically analyzed to evaluate phytopharmacological potential and limitations on developing novel herbal products in Sri Lanka. Based on the available data, <em>O. sanctum</em> contains various bioactive flavonoids, terpenoids, and phenolic compounds. It could be a renewable source of valuable phytochemicals, including eugenol, methyl eugenol, β-caryophyllene, rosmarinic acid, and apigenin, with high potential to be utilized in various herbal products, including antifungal and anti-inflammatory topical applications and anti-aging cosmeceuticals. <em>P. amboinicus</em> contains phenolics (thymol and carvacrol), flavonoids, and abietene diterpenoids as its predominant phytochemicals. They possess a wide range of biological properties and have high potential in developing novel herbal products related to treating and preventing a wide range of health conditions. The data suggest that both species have substantial potential for inclusion in novel evidence-based herbal products. Both plants exhibit rapid growth and can be easily cultivated, making them ideal candidates for cost-effective and scalable herbal product development. However, challenges related to standardization, quality control, and market acceptance have been identified in Sri Lanka. To overcome these challenges, efforts focused on enhancing research, acknowledgment programs, and commercialization strategies in the herbal product sector are recommended. By utilizing the medicinal properties of <em>O. sanctum</em> and <em>P. amboinicus</em>, Sri Lanka can emerge as a global leader with evidence-based phytomedicine research and present a sustainable economic development.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100920"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyherbal formular 5 normalises blood glucose level and reverses complications in diabetes via the upregulation of AMPKinase, GLUT 1 and GLUT 4 activities in albino rats","authors":"Gavin C. Ikechukwu ,&nbsp;Simeon I. Egba ,&nbsp;Emmanuel N. Uhuo ,&nbsp;Humphrey C. Omeoga ,&nbsp;Polycarp N. Okafor ,&nbsp;Patricia E. Mbah","doi":"10.1016/j.phyplu.2025.100939","DOIUrl":"10.1016/j.phyplu.2025.100939","url":null,"abstract":"<div><div>Diabetes mellitus remains a major global health challenge, and the search for safer, plant-based therapeutic options continues to gain momentum. This study investigated the molecular and biochemical mechanisms underlying the antidiabetic activity of a novel poly-herbal formula (PHF5), prepared in a defined ratio from the leaves of <em>Ocimum gratissimum, Vernonia amygdalina, Gongronema latifolium, Gnetum africanum,</em> and <em>Aloe barbadensis</em>. Alloxan-induced diabetic male Wistar rats were assigned to six groups: normal control, diabetic untreated, diabetic rats treated with PHF5 (75, 150, or 300 mg/kg body weight), and diabetic rats treated with Glibenclamide (5 mg/kg). Treatment lasted for five weeks with periodic monitoring of fasting blood glucose. PHF5 significantly reduced hyperglycemia, restored insulin levels, and enhanced AMPKinase, GLUT1, and GLUT4 activities in diabetic rats. These results demonstrate that PHF5 exerts potent antidiabetic effects, likely through the upregulation of the AMPKinase/GLUT4 signaling pathway. The study provides novel mechanistic insight into the synergistic potential of combined ethnomedicinal plants and supports PHF5 as a promising candidate for diabetes management.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100939"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AB-free kava suppresses tobacco smoke-induced CREB phosphorylation in the mouse cerebellum","authors":"Yifan Wang ,&nbsp;Tara Hashemian ,&nbsp;Tengfei Bian ,&nbsp;Leah R. Reznikov ,&nbsp;Adriaan W. Bruijnzeel ,&nbsp;Tuo Lin ,&nbsp;Chengguo Xing","doi":"10.1016/j.phyplu.2025.100940","DOIUrl":"10.1016/j.phyplu.2025.100940","url":null,"abstract":"<div><h3>Introduction</h3><div>Our recent studies revealed the potential of AB-free kava as a novel therapeutic candidate against tobacco use disorder while the underlying mechanism remains to be elucidated. The cerebellum region in the brain has been reported to be involved in tobacco withdrawal. The cAMP response element-binding protein (CREB) activation via phosphorylation has also been reported to contribute to substance abuse, including tobacco use disorder. Our early pre-clinical work revealed the potential of AB-free kava to suppress tobacco smoke-induced CREB activation in lung tissues.</div></div><div><h3>Methods</h3><div>In this study, we investigated the impact of tobacco smoke exposure on the levels of phosphorylated CREB (p-CREB) in the mouse cerebellum and evaluated the effects of AB-free kava.</div></div><div><h3>Results</h3><div>Four different regimens of tobacco smoke exposure consistently increased the levels of p-CREB in the cerebellum. AB-free kava effectively suppressed tobacco smoke-induced increase in p-CREB to levels comparable to mice without tobacco smoke exposure.</div></div><div><h3>Conclusions</h3><div>Our data provide preliminary evidence that CREB activation in the cerebellum is a potential mechanism involved in tobacco use disorder and the protective potential of AB-free kava.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100940"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From tradition to therapeutics: antioxidant, anti-inflammatory, and antidiabetic activities of Garcinia cowa Roxb. leaf extracts","authors":"Kalyani Pathak ,&nbsp;Aparoop Das ,&nbsp;Monjit Das ,&nbsp;Riya Saikia ,&nbsp;Mohammad Zaki Ahmad ,&nbsp;Jon Jyoti Sahariah ,&nbsp;Manash Pratim Pathak ,&nbsp;Md Ariful Islam ,&nbsp;Pallab Pramanik ,&nbsp;Sukanya Sonowal","doi":"10.1016/j.phyplu.2026.100955","DOIUrl":"10.1016/j.phyplu.2026.100955","url":null,"abstract":"<div><div>This study scientifically evaluated the antioxidant, anti-inflammatory, and antidiabetic potential of <em>Garcinia cowa</em> Roxb. leaf extracts, a plant widely used in Assamese traditional medicine but lacking strong pharmacological validation. Successive extracts were prepared using solvents of increasing polarity, and their total phenolic and flavonoid contents were quantified. The methanol extract showed the highest phenolic (0.317 ± 0.02 mg/mL) and flavonoid (0.208 ± 0.01 mg/mL) concentrations, which correlated with potent antioxidant activity in DPPH (IC₅₀ = 70.25 µg/mL), hydrogen peroxide (IC₅₀ = 24.80 µg/mL), and nitric oxide (IC₅₀ = 89.12 µg/mL) radical scavenging assays. In vivo evaluation using carrageenan-induced paw edema revealed notable anti-inflammatory activity. Antidiabetic assessment through α-glucosidase inhibition demonstrated significant enzyme inhibition by the methanol extract (IC₅₀ = 42.00 µg/mL), while studies in streptozotocin-induced diabetic rats showed a marked reduction in blood glucose levels over 21 days (131.5 mg/dL on Day 21), approaching the efficacy of the standard drug Miglitol (110.32 mg/dL). Histopathological examination indicated preservation of pancreatic islet architecture in extract-treated groups, supporting its therapeutic potential. Acute toxicity studies established safety up to 5000 mg/kg, suggesting a wide margin of safety. Overall, the methanol extract of <em>Garcinia cowa</em> leaves exhibited strong antioxidant, anti-inflammatory, and antidiabetic activities, scientifically supporting its ethnomedicinal use and indicating promise as a natural source for developing safe, effective plant-based therapeutics. Further work is required to isolate and characterize the responsible bioactive compounds.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100955"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical, antioxidant, anthelmintic and analgesic potential of aqueous and methanolic extracts of Selaginella vogelii","authors":"Calvin Bogning Zangueu ,&nbsp;Marie Claire Tchamadeu ,&nbsp;Jacquy Joyce Kojom Wanche ,&nbsp;Philippe Belle Ebanda Kedi ,&nbsp;Augustine Kuinze Nkojap ,&nbsp;Russelle Camelie Nguemnang Tchatchouang ,&nbsp;William Yousseu Nana ,&nbsp;Fabrice Fabien Dongho Dongmo ,&nbsp;Eulojio J Llorent-Martinez ,&nbsp;Edwige Laure Nguemfo ,&nbsp;Alain Bertrand Dongmo","doi":"10.1016/j.phyplu.2025.100954","DOIUrl":"10.1016/j.phyplu.2025.100954","url":null,"abstract":"<div><h3>Background</h3><div><em>Selaginella vogelii</em> is a vascular plant widely distributed in tropical Africa and well known for its medicinal potential.</div></div><div><h3>Purpose</h3><div>With a view to find its phytochemical composition and pharmacological evidence, the aqueous and methanolic extracts from leaves, stems and roots mixture was evaluated.</div></div><div><h3>Methods</h3><div>Comparative phytochemicals analysis of leaves, stems and roots mixture was carried out by HPLC-ESI-MSⁿ, as well as <em>in vitro</em> assessment of antioxidant, anthelmintic and in vivo assessment of analgesic activity using standard methods.</div></div><div><h3>Results</h3><div>Twenty-two (22) and 24 compounds respectively from leaves, stems and roots extracts were identified. Flavonoids, saponins and phenolic acids, were the main classes of secondary metabolites. <em>S. vogelii</em> extracts demonstrated antiradical activity against DPPH, ABTS and NO as well as developing a ferric reducing power, thus revealing that methanolic extract have a higher antioxidant potential. <em>S. vogelii</em> showed ovicidal and larvicidal activities against eggs and infective larvae of <em>Haemonchus contortus</em>. The half-maximal inhibitory concentrations were 411.8 and 129 µg/ml, respectively in eggs hatching test and larval migration inhibition assay. Significant antinociceptive activity against various pain models was exhibited by <em>S. vogelii</em> extracts. The reduction of the number of abdominal contortions following intraperitoneal injection of acetic acid was observed, with maximum inhibition of 53.9 % (<em>p</em> &lt; 0.01) recorded for methanolic extracts of stems and roots mixture at the dose of 400 mg/kg. Additionally, significant reduction (<em>p</em> &lt; 0.01) of paw licking time was observed following subplantar injection of formalin, equivalent to a maximum inhibition of 61.95 % for the neurogenic pain and 69.34 % for the inflammatory pain respectively, with methanolic leaves extract. The plant has also significantly inhibited the nociceptive response provoked by tail immersion in hot water and by the subplantar injection of capsaicin or cinnamaldehyde in mice. Therefore suggesting that anti-nociceptive action of this plant could result from the inhibition of the release of endogenous mediators, the inhibition of arachidonic acid metabolism and by regulating the activation of the vanilloid receptor or inotropic channel.</div></div><div><h3>Conclusions</h3><div>These findings highlight the biological activities and the plant’s chemical composition potential.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100954"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of polyherbal formulations in regenerating wounds and profiling their therapeutic properties was tracked via high-throughput methods in conjunction with In silico molecular docking","authors":"Mekala K,&nbsp;Shaheedha SM","doi":"10.1016/j.phyplu.2025.100950","DOIUrl":"10.1016/j.phyplu.2025.100950","url":null,"abstract":"<div><div>Wound healing is a complicated process that includes detailed interactions between cells and molecules. This study investigated the burn wound healing properties of a polyherbal formulation obtained from Trigonella, Lawsonia, Basella, Peristrophe and Purslane.Qualitative phytochemical examination revealed that the extract is mostly composed of phenolic components, indicating its potential medicinal benefits. <em>In vitro</em> wound healing experiments on L929 cell lines revealed a notable increase in cell proliferation and migration after treatment with the polyherbal formulation compared to the monoherbal extract, suggesting its effectiveness in accelerating wound closure. Apigenin, coumaric acid, diosmetin, ferulic acid, lalioside, luteolin, protocatechuic acid, syringic acid and vitexin were the predominant bioactive components in the polyherbal formulation according to liquid chromatography-high-resolution mass spectrometry (LC<img>HRMS).Molecular docking simulations via AutoDock software revealed robust binding interactions between key components of the polyherbal formulation and wound healing receptors, such as fibroblast growth factor (4V05), epidermal growth factor receptor (6Z4B), and human matrix metalloproteinase (3SHI), indicating its possible role in wound healing mechanisms. A deeper analysis of the binding interactions showed that luteolin exhibited strong binding affinity with receptor 3SHI (-9.00 kcal/mol) due to its ability to form hydrogen bonds and hydrophobic interactions at the active site, which are critical for modulating collagenase activity during wound healing. Apigenin and vitexin also showed significant interactions with 4V05 and 6Z4B, suggesting their potential roles in promoting fibroblast proliferation and epidermal repair, respectively. These findings are supported by literature demonstrating the biological relevance of these binding interactions in wound repair pathways.Research indicates that the polyherbal formulation, which contains flavones, glycosides, and phenolic compounds, has potential as a wound healing agent because it influences important molecular targets in wound repair pathways. Additional research is needed to clarify the exact mechanisms responsible for the reported benefits and to investigate its potential therapeutic uses in clinical environments.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100950"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The hypoglycemic and hypolipidemic effects of Olea europaea L. leaves on fructose-induced diabetic wistar rats","authors":"Ibrahim Hinad,&nbsp;Youssef S’hih,&nbsp;Abdelhalem Mesfioui,&nbsp;Aboubaker Elhessni,&nbsp;Moulay Laarbi Ouahidi","doi":"10.1016/j.phyplu.2025.100934","DOIUrl":"10.1016/j.phyplu.2025.100934","url":null,"abstract":"<div><div>Type 2 Diabetes mellitus is a chronic disorder of carbohydrates, protein and lipid metabolism ending up with serious complications such retinopathy, nephropathy and neuropathy. <em>Olea europaea</em> L. leaves are extensively used worldwide in conventional medicine of several diseases, especially Diabetes mellitus. The purpose of the present study was to assess the influence of olive leaves extract on normal and fructose-induced diabetic male rats. To assess the hypoglycemic effect of olive leaves extract, five groups of rats were formed. The first was used as normal control (treated with distilled water), the second was normal treated with a dose of 500mg/kg.bw of the extract, the third was diabetic control (treated with distilled water), the fourth and fifth groups were diabetic groups administered with the extract (500 mg/kg.bw) and anti-diabetic drug (25 µg/kg.bw) respectively. The treatment lasted 28 days during which the variations of fasting blood glucose, body weight, the food and water consumption were reported and total Cholesterol and Triglycerides were measured for all groups at the end of the treatments. The extract of olive leaves has shown a remarkable anti-hyperglycemic activity accompanied by a significant decrease (<em>p</em> &lt; 0.05) in the body weight of rats. In addition, chronic treatment of rats by the extract improved the lipid parameters of diabetic rats. The present results proved the hypoglycemic and hypolipidemic activities of the olive leaves extract and confirmed its use in traditional treatment of diabetes. However, additional investigations are needed to isolate the active compounds responsible for these activities, identify its mode of action and enhance it for a safe and a successful usage.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100934"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Moringa Oleifera leaf extract on glycemic control and inflammation in metabolic syndrome: a randomized controlled trial","authors":"Mohammad Rashidmayvan ,&nbsp;Naseh Pahlavani ,&nbsp;Mohammad Ali Javanmardi ,&nbsp;Alireza Gheflati ,&nbsp;Mojgan Mehri Ardestani ,&nbsp;Saeid Hadi ,&nbsp;Vahid Hadi","doi":"10.1016/j.phyplu.2025.100932","DOIUrl":"10.1016/j.phyplu.2025.100932","url":null,"abstract":"<div><h3>Background</h3><div>The present double-blind, randomized, placebo-controlled clinical trial aimed to evaluate the effects of <em>Moringa oleifera (MO)</em> extract, a natural source of polyphenols, on glycemic control and inflammatory markers in patients with metabolic syndrome (MetS).</div></div><div><h3>Methods</h3><div>Totally 73 MetS diagnosed patients were randomly assigned to receive either 1000 mg of <em>MO</em> supplement (n = 37) or 1000 mg twice a day placebo (n = 36) for 8 weeks. Inflammatory and oxidative stress markers, blood pressure and glycemic indices were assessed at the beginning and at the end of the study. Also anthropometric indices, dietary intake and physical activity levels of participants were assessed in the beginning of the study.</div></div><div><h3>Results</h3><div>Our study revealed that <em>MO</em> supplementation had a significant effect on serum levels of Glycosylated hemoglobin A1c (HbA1c) and insulin (P = 0.07 and p = 0.13, respectively). Interestingly, the MO group reduced high-sensitive reactive protein (hs-CRP) and malondialdehyde (MDA) after 8 weeks (P = 0.04 and P = 0.02 respectively) compared to the control group, we did not observe any significant effects on serum levels of tumor necrosis factor alpha (TNF-α), erythrocyte sedimentation rate (ESR), and interleukin 6 (IL-6) in patients who received <em>MO</em> after 8 weeks of intervention. Additionally, we have not found significant associations between weight, body mass index (BMI), waist circumference (WC), and <em>MO</em> supplementation among patients with MetS. MO supplementation reduced HbA1c by 0.4% (P=0.02) and insulin by 1.2 μIU/mL (P=0.011), with no significant changes in body weight (mean difference: −0.5 kg, P=0.21). furthermore, in the <em>MO</em> group, systolic and diastolic blood pressure decreased, but these effects weren’t significant.</div></div><div><h3>Conclusion</h3><div><em>MO</em> supplementation may serve as a complementary therapy for MetS patients, given its beneficial effects on both inflammatory markers and glycemic control.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100932"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}