Herbal tea prepared in-house shows potential immunomodulatory activity in cyclophosphamide-induced myelosuppression in rats

Abstract

Background

Withania somnifera, Tinospora cordifolia and Glycyrrhiza glabra, popular herbs in Ayurveda, have been reported to modulate and enhance immune functions. Similarly, Ocimum sanctum, Ocimum gratissimum and Camelia sinensis have also been used as immunomodulatory agents in Ayurvedic system of medicine. Considering the individual pharmacological properties of these six above-mentioned plants, we prepared herbal tea (HT) and evaluated its immunomodulatory effects.

Objective

This study aimed at establishing the immunomodulatory potential of in-house herbal tea, both in vitro and in vivo.

Material and methods

Aqueous infusion of HT was prepared and its total phenolic and total flavonoid content was determined. Phytochemical analysis was performed using UPLCHRMS. The aqueous extract was then evaluated for in vitro cytotoxicity and Nitric oxide release in RAW 264.7 cells. The immunomodulation potential of HT in vivo was studied in male/female SD rats and the model was developed by using cyclophosphamide (CTX).

Results

Phytochemical analysis of HT showed the presence of gallic acid, glycyrrhizin, catechin, epigallocatechin gallate, vicenin and chlorogenic acid. HT1 (100 mg/kg) could significantly inhibit reduction in body weight and weight of thymus and spleen. Also, it improved the leucocyte count, both total and differential, phagocytic index, neutrophil adhesion and level of IL-1β (47.12 %) and IL-6 (44.77 %). Further, the HT prevented destruction of normal vascular organization and cellular composition of spleen and thymus tissues.

Conclusion

The findings of the study suggest that the immune booster herbal tea at the dose 100 mg/kg, ameliorates CTX-induced myelosuppression and immunosuppression in rats and possesses potent immunomodulatory activity.