Journal of Agriculture and Food Research最新文献

{"title":"Glutathione-rich yeast extract improves alcohol-associated liver diseases through the improvement of mitochondrial dysfunction and oxidative stress by activating SIRT3","authors":"Hao Han ,&nbsp;Yanyang Han ,&nbsp;Yajing Dong ,&nbsp;Shuqi Ji ,&nbsp;Xiaoman Wang ,&nbsp;Yuantong Yu ,&nbsp;Xingxing Che","doi":"10.1016/j.jafr.2026.102735","DOIUrl":"10.1016/j.jafr.2026.102735","url":null,"abstract":"<div><div>Alcohol-associated liver diseases (ALD) is a chronic liver disease characterized by mitochondrial dysfunction and oxidative stress. Glutathione (GSH)-rich yeast extract (GYE) is a product abundant in proteins, amino acids, fiber, trace elements, and GSH. Previous studies have reported that GYE exhibits anti-inflammatory and antioxidant properties. However, the effect of GYE on ALD and its underlying mechanisms remain largely unclear. In the present study, we investigated whether GYE could protect against ALD using ethanol-treated mice and HepG2 cells, with a specific focus on mechanisms involving the amelioration of ethanol-induced mitochondrial damage and oxidative stress. The results showed that GYE significantly ameliorated liver injury in chronic ethanol-feeding mice. In addition, GYE increased the content of GSH in the liver, thereby protecting against ethanol-induced oxidative stress and mitochondrial dysfunction. Moreover, the expression of SIRT3 was measured to reveal potential targets by which GYE protected against ALD. Our results showed that the ethanol-induced inhibition of SIRT3 expression was reversed by GYE in vivo and in vitro. Silencing of SIRT3 by siRNA markedly weakened the protective effect of GYE on mitochondrial function and oxidative stress. Our findings suggest that GYE alleviates ethanol-induced mitochondrial damage and oxidative stress via activating SIRT3 in an ALD mouse model, which highlights a promising prevention strategy for ALD.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102735"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management practices and species interactions drive yield increases through complementarity and selection effects in L. iberica–chickpea intercropping systems","authors":"Maryam Mirdoraghi ,&nbsp;Saeideh Maleki Farahani ,&nbsp;Alireza Rezazadeh","doi":"10.1016/j.jafr.2025.102627","DOIUrl":"10.1016/j.jafr.2025.102627","url":null,"abstract":"<div><div>Ensuring food security amid global population growth requires sustainable agricultural practices such as intercropping, which enhances resource efficiency and yield by cultivating multiple species together. This study assessed intercropping performance using key indices, including the Biodiversity Effect (BE), under three irrigation regimes—severe deficit irrigation (I_S), complete irrigation (I₂₀), and moderate deficit irrigation (I₄₀)—as well as two sowing dates—autumn (S₁) and spring (S₂)—and two cropping systems: sole system (Ss) versus 50:50 intercropping (Ic). The results showed that the Ic (I₂₀S₁) treatment achieved maximum productivity, with a LER of 2.43, a monetary advantage index (MAI) of 6565.25, and total land output (TLO) of 3762.3 kg ha⁻¹. Complementary effects (CE)—driven by chickpea (<em>Cicer arietinum</em> L.) deeper root system, nitrogen fixation, and spatiotemporal niche differentiation with <em>L. iberica</em> (<em>Lallemantia iberica</em>)—helped mitigate drought stress and improve resource-use efficiency. In the intercropping system, CE outweighed selection effects (SE), with intercropping benefits mainly attributed to CE, which strongly correlated with BE. Chickpea dominated the intercrop (competitive ratio: CRc &gt; CRl), with its yield peaking under conditions of enhanced nitrogen availability through symbiotic fixation and facilitation. Meanwhile, I_SS₂ conditions reduced sole system productivity by 45 %, but intercropping remained resilient by leveraging nitrogen synergy and reducing intraspecific competition. Autumn sowing enhanced nitrogen uptake and economic returns (RVT: 5.97) by aligning with cooler temperatures and seasonal rainfall. However, the Ic (I_SS₂) treatment resulted in economic losses (−26.3 % TLO) and shifted species interactions towards competition, reducing LER by 45 %. These findings highlight intercropping as a sustainable, low-input strategy under optimal irrigation and sowing conditions, offering critical insights for climate-resilient agriculture. Long-term adaptation strategies will be essential to maintaining these synergies amid increasing environmental challenges.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102627"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservation agriculture and cover crops enhances yield and profitability of grain production systems","authors":"Lutécia Beatriz Canalli ,&nbsp;Laíse da Silveira Pontes ,&nbsp;Guilherme Leonardi ,&nbsp;Marcelo Augusto de Aguiar e Silva ,&nbsp;Tiago Santos Telles","doi":"10.1016/j.jafr.2026.102672","DOIUrl":"10.1016/j.jafr.2026.102672","url":null,"abstract":"<div><div>This study aimed to verify whether diversified crop rotation systems are more productive and profitable than the wheat-soybean crop rotation system. In southern Brazil, five crop rotation systems with varying levels of species diversification (ranging from 4 to 10 species per three-year cycle) were compared to a wheat-soybean crop rotation system over six agricultural years (2017–2018 to 2022–2023, in two three-year cycles). Yield and economic indicators were evaluated. At the end of the six agricultural years, all treatments were profitable. The highest accumulated profit was observed in the treatment that rotated corn, bean, and soybean in summer with cover crops and cereals in winter. This system was 38 % more profitable than the wheat-soybean crop rotation system. The results suggest that crop rotation with species diversification, especially when incorporating cover crops in conservation agriculture, can be more profitable, enhance soybean yields, and require lower investments compared to traditional wheat-soybean crop rotation system.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102672"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive growth stages according to the extended BBCH-scale and physical-chemical changes of yellow pitahaya fruit (Hylocereus megalanthus)","authors":"Ives Yoplac ,&nbsp;River Chávez ,&nbsp;Laydy M. Mena ,&nbsp;Jorge L. Maicelo-Quintana ,&nbsp;Alyssa Hidalgo","doi":"10.1016/j.jafr.2026.102699","DOIUrl":"10.1016/j.jafr.2026.102699","url":null,"abstract":"<div><div>The yellow pitahaya (<em>Hylocereus megalanthus</em>), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102699"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic and proteomic profiling of royal jelly from honeybee (Apis mellifera) colonies fed with camellia versus rapeseed bee pollen","authors":"Hangfei Han ,&nbsp;Xinhai Liu ,&nbsp;Jilian Li ,&nbsp;Chuan Ma","doi":"10.1016/j.jafr.2026.102669","DOIUrl":"10.1016/j.jafr.2026.102669","url":null,"abstract":"<div><div>Royal jelly (RJ), a bioactive secretion of honeybees (<em>Apis mellifera</em>), is a functional food valued for its nutritional and therapeutic properties. Its physicochemical properties are critically influenced by pollen sources, a primary diet for honeybees. In this study, we investigated compositional changes of RJ produced from colonies fed camellia bee pollen (CBP) versus rapeseed bee pollen (RBP) through integrated metabolomic and proteomic approaches. We observed no significant differences in larval acceptance rate or RJ yield between the two RJ groups on feeding days 63, 66, and 69 (<em>p</em> &gt; 0.05). Principal component analysis (PCA) of metabolome data revealed an increasingly pronounced compositional divergence between the two RJ groups harvested on days 9, 21, 42, and 63. While <em>trans</em>-10-hydroxy-2-decenoic acid (10-HDA) and major sugars (glucose, fructose, and sucrose) exhibited comparable abundance, RBP-RJ contained significantly higher abundance of multiple bioactive compounds, including kaempferol, lysine, taurine, trigonelline, and acetyltaurine, indicating superior nutritional value and potential functional benefits. Proteomic analysis of day-63 samples identified only nine low-abundance differentially expressed proteins (0.31 % of total protein abundance), with no significant differences in major RJ protein (MRJP) levels. Our multi-omics findings highlight the compositional superiority of RBP-RJ, offering insights for optimizing dietary strategies in high-quality RJ production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102669"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of maternal metabolism, gut homeostasis, and key microbiota by pre-pregnancy and gestational high-energy diet-induced maternal obesity","authors":"Tianle He ,&nbsp;Jiani Mao ,&nbsp;Ju Luo ,&nbsp;Suping Ding ,&nbsp;Jundan Zheng ,&nbsp;Zhenguo Yang","doi":"10.1016/j.jafr.2026.102710","DOIUrl":"10.1016/j.jafr.2026.102710","url":null,"abstract":"<div><div>This study aimed to investigate how obesity induced by a high-energy diet (HED) during pregnancy affects maternal gut microbiota, energy metabolism, intestinal barrier function, and body fat remodeling using C57BL/6J mice as the model. Results show that HED significantly increased maternal weight gain and adiposity and increased maternal body fat percentage (<em>P</em> &lt; 0.01). Serum biochemical indicators showed that HED elevated the energy metabolic burden on pregnant mothers (<em>P</em> &lt; 0.01). Notably, HED inhibited fat type conversion during pregnancy, worsening maternal obesity (<em>P</em> &lt; 0.01). Physical damage to the maternal liver and small intestine was observed, activating gut immunity and reducing gut antioxidant properties (<em>P</em> &lt; 0.01). However, increased expression of fatty acid transporters in the small intestine and an elevated villus height to crypt depth ratio accelerated maternal fatty acid absorption (<em>P</em> &lt; 0.01). Significant alterations in the maternal gut microbiota were identified, with key microorganisms such as <em>Adlercreutzia, Anaerotruncus, Bifidobacterium, Roseburia, Alistipes, Mucispirillum, Lactococcus, Ruminococcus, Bacteroides,</em> and <em>Allobaculum</em> showing correlations with maternal energy metabolism, gut health, and body fat conversion. This study elucidated the impact of HED on maternal gut microbiota, energy metabolism, gut health, and body fat conversion during pregnancy, highlighting a significant connection between gut microbiota and maternal health.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102710"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From mechanisms to networks: A global bibliometric and functional synthesis of Trichoderma in plant disease management","authors":"Anthony Apolinario Cortez-Lázaro ,&nbsp;Jeremy Israel Chavez-Castillo ,&nbsp;José Luis Romero-Bozzetta ,&nbsp;Jorge Luis Rojas-Paz ,&nbsp;Gabriel Alberto Manes-Cangana ,&nbsp;Ronald Alexis Cortez-Lázaro ,&nbsp;Fredesvindo Fernandez-Herrera ,&nbsp;Edson Max Caro-Degollar ,&nbsp;Mirtha Soledad Ferrer-Ventocilla ,&nbsp;Ronnel Edgar Bazan-Bautista ,&nbsp;Jennifer Valeria García-Evangelista","doi":"10.1016/j.jafr.2026.102706","DOIUrl":"10.1016/j.jafr.2026.102706","url":null,"abstract":"<div><div>Fungal pathogens remain a major constraint to global food production, while resistance to chemical fungicides continues to increase. Although <em>Trichoderma</em> spp. are extensively studied as microbial biocontrol agents, no integrated synthesis has yet connected the field's bibliometric growth with its mechanistic and technological foundations. This study analyzed 3082 Scopus-indexed publications (1975–2024) using R (bibliometrix) and VOSviewer to track publication trends, global collaboration networks, and thematic evolution. Results were linked to primary literature on molecular mechanisms and field performance. Annual output grew from 39 (2000) to 285 (2023), with an 8.6 % compound annual growth rate. Collaboration patterns reveal two central axes India-Eastern Mediterranean and China-United States aligned with national bioinput agendas. Thematic clusters show a transition from single-strain antagonism to integrative approaches involving omics, microbial ecology, and formulation design. <em>Trichoderma</em> efficacy is driven by antibiosis, enzymatic lysis, and hormone-mediated defense, shaped by rhizosphere conditions and formulation matrices. Advances in encapsulation, osmoprotection, and chemical compatibility have improved field persistence and efficacy. This study identifies the need for standardized reporting, omics-informed strain selection, and regulatory-aligned formulation strategies to improve the reproducibility, scalability, and adoption of <em>Trichoderma</em>-based biocontrol as a resilient component of sustainable agriculture.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102706"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of phosphofructokinase-1 as a target of Chinese olive fruit (Canarium album L.) extract in colorectal cancer cells: Mechanistic insights into cellular energy stress-induced apoptosis","authors":"Guan-Ting Lian ,&nbsp;Chun-Wai Chan ,&nbsp;Hsiu-Hsiang Lee ,&nbsp;Yi-Chun Liao ,&nbsp;Chen-Chung Liao ,&nbsp;Shu-Chen Hsieh","doi":"10.1016/j.jafr.2026.102684","DOIUrl":"10.1016/j.jafr.2026.102684","url":null,"abstract":"<div><div>Colorectal cancer (CRC) is a significant cause of global cancer mortality. While conventional chemotherapeutics or single-agent compounds can suppress tumor growth, their dose-limiting toxicities often erode patient survival and quality of life. Plant extracts offer a distinct therapeutic advantage, leveraging multi-targeted pathways with inherently lower toxicity profiles. Here, we report the multi-signal transduction pathway of Chinese olive extract (COE) mediated CRC apoptosis. COE selectively inhibits CRC cell growth, with GI₅₀ values significantly lower than in normal intestinal epithelial cells. Quantitative proteomics revealed COE-driven perturbations in cell cycle regulation, apoptosis, and metabolic reprogramming. Thermal proteome profiling and cellular thermal shift assay identified phosphofructokinase-1 (PFK1), a key glycolytic enzyme, as a direct molecular target of COE. Interrogation of The Cancer Genome Atlas data revealed that elevated PFK1 expression was linked to poorer CRC patient survival. Mechanistic studies demonstrated that COE suppresses PFK1 enzymatic activity, leading to cellular energy stress and activation of the adenosine monophosphate-activated protein kinase (AMPK)/p53 axis to promote apoptosis. Notably, amentoflavone, a biflavonoid constituent of COE, was computationally predicted and experimentally validated to inhibit PFK1, recapitulating COE's anti-proliferative effect. Collectively, our findings reveal that, besides regulating cell cycle and apoptosis, COE activates a previously unrecognized PFK1–AMPK/p53 signaling pathway, providing a mechanistic rationale for its development as a metabolism-oriented therapeutic strategy in CRC.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102684"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-agronomic and biochemical diversity of the Tomata Valenciana landrace reveal key traits for its revalorisation and subtype differentiation","authors":"Ramón Gabriel Rico Ferrer,&nbsp;María del Rosario Figàs,&nbsp;Nicolás Arroyo,&nbsp;Miquel Martínez,&nbsp;Elena Soler,&nbsp;Cristina Casanova,&nbsp;Jaime Prohens,&nbsp;Salvador Soler","doi":"10.1016/j.jafr.2026.102717","DOIUrl":"10.1016/j.jafr.2026.102717","url":null,"abstract":"<div><div>The Tomata Valenciana tomato (<em>Solanum lycopersicum</em> L.) landrace, valued for its local importance and unique organoleptic qualities, shows notable intra-varietal diversity. In this study, 46 accessions from three subtypes (‘Blanca’, ‘Masclet’, and ‘Intermitja’) were assessed using 57 morpho-agronomic and 15 biochemical descriptors. High phenotypic variation was observed, particularly in fruit morphology and agronomic traits. Features like fruit weight (FWH), locule number (LCN), and fruit tip formation (FTF) showed high heritability (<em>H</em>^<em>2</em> &gt; 0.6). Principal Component Analysis clearly separated subtypes based on morphology: ‘Blanca’, which had larger, rounder fruits with lighter immature colour; ‘Masclet’, with darker green shoulders and pointed tips; and ‘Intermitja’, which showed intermediate traits and greater plant vigour. In contrast, biochemical traits displayed reduced variation among subtypes and low <em>H</em>^<em>2</em>. These findings highlight the morphological and agronomic diversity of Tomata Valenciana, especially in fruit traits that are valuable for breeding and varietal authentication. The identification of stable, differentiating traits supports conservation efforts and legal recognition through quality labels. The unique profile of the ‘Intermitja’ subtype provides new insights into possible hybridization processes within landraces and their contribution to maintaining genetic diversity in traditional farming systems.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102717"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical discrimination and market-oriented qualitative traits of a 24 lemon cultivar collection","authors":"Giulia Modica ,&nbsp;Luana Pulvirenti ,&nbsp;Alessandra Gentile ,&nbsp;Giuseppe Ruberto ,&nbsp;Laura Siracusa ,&nbsp;Alberto Continella","doi":"10.1016/j.jafr.2026.102658","DOIUrl":"10.1016/j.jafr.2026.102658","url":null,"abstract":"<div><div>Lemon [<em>Citrus limon</em> (L.) Burm. f.] is an important species cultivated in several citrus-producing countries. Lemon fruits are usually used for fresh consumption and for processed juice due to their excellent nutraceutical properties attributed to their high levels of bioactive compounds, mainly specialized (secondary) metabolites. A total of 24 cultivars were studied (20 belonging to the Italian germplasm plus 4 international varieties) for their morphological parameters, specialized metabolite content evaluated by HPLC/Uv–Vis/DAD and UPLC/ESI/Orbitrap MS analyses and antioxidant activity of juices assessed by ABTS⁺ and DPPH^• tests. State of the art statistical tools were used to rationalize the data, specifically Principal Component Analysis (PCA) and clustering analysis, Random Forest and Heatmap were performed. The results obtained allowed us to assess biochemical variability and to identify a commercial placement (fresh market vs industrial transformation) for the analyzed genotypes. The results indicated that F. Adamo, F. Scandurra, F. Continella, F. Cerza and F. Akragas could be suitable for the fresh consumption due to their favorable morphological parameters. Conversely, F. Siracusano, F. Cocuzzaro and F. S.Teresa, F. 2Kr and F. Fragalà were distinguished by their high levels of metabolic compounds. Lemox and Fino 49 exhibited the largest fruit weight, suggesting their potential use for processed juice production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102658"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}