Gergő Szabó , Zsuzsanna Horváth-Mezőfi , Lien Le Phuong Nguyen , Stephen Tully , Zoltán Gillay , Christopher Brian Watkins , Tamás Zsom , Géza Hitka
{"title":"Determination of specific gas exchange rates in microperforated modified atmosphere packaging: effect of perforation size and gas concentration","authors":"Gergő Szabó , Zsuzsanna Horváth-Mezőfi , Lien Le Phuong Nguyen , Stephen Tully , Zoltán Gillay , Christopher Brian Watkins , Tamás Zsom , Géza Hitka","doi":"10.1016/j.jafr.2025.102118","DOIUrl":"10.1016/j.jafr.2025.102118","url":null,"abstract":"<div><div>The modified atmosphere packaging for minimally processed or fresh-cut horticultural products, such as fruits and vegetables, presents significant challenges for the food industry. Since fruits remain biologically active after harvest, they require packaging materials that allow for proper gas exchange. The optimal packaging solution depends on factors such as the gas exchange area of the packaging film, temperature, package volume, product weight, and the respiration rate of the stored produce. This study investigates the effects of laser-induced microperforations in 65 μm polypropylene plastic films on the gas exchange rates of key gases (CO<sub>2</sub>, O<sub>2</sub>, N<sub>2</sub>). Two different perforation sizes were tested, with perforated areas ranging from 0.017 to 0.041 mm<sup>2</sup>. Gas exchange rates were calculated using a polynomial function based on the concentration differences of the gases. The distinctive characteristic of the present study is that it refines the results obtained thus far in relation to laser perforations and their dimensions, which can be utilised for industrial applications.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102118"},"PeriodicalIF":4.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Liang , Shutian Sun , Xinpei Qi , Zhenyu Han , Huijue Kong , Difei Luo , Xiaoyu Luo
{"title":"Deteriorative reactions of Pacific whiteleg shrimp (Litopenaeus vannamei) during chilled storage: enzymatic degradation, microbial activity, and quality changes","authors":"Jun Liang , Shutian Sun , Xinpei Qi , Zhenyu Han , Huijue Kong , Difei Luo , Xiaoyu Luo","doi":"10.1016/j.jafr.2025.102117","DOIUrl":"10.1016/j.jafr.2025.102117","url":null,"abstract":"<div><div>Pacific whiteleg shrimp (<em>Litopenaeus vannamei</em>) is one of the signature aquatic products in the city of Zhuhai and the Greater Bay area of southern China. Due to its high perishability under ambient conditions, the deterioration during storage can result in the decline of eating quality and nutrient loss. This study investigated the deteriorative mechanisms of Pacific whiteleg shrimp during 7-day storage at 4 °C, especially on enzymatic autolysis, oxidative reactions, and microbial growth. Comparing with fresh shrimp, on day 7, significant higher pH (7.70), total volatile basic nitrogen content (81.32 mg/100 g), and peroxide value (17.09 mg hydroperoxide/kg) were found (<em>p</em> < 0.05), demonstrating the accumulation of alkaline amine metabolites and higher degree of lipid oxidation. Shrimp protein degradation was revealed by the increase of peptides, loss of sulfhydryl groups, as well as activity decline of endogenous enzymes. <em>Vibrio, Photobacterium</em>, <em>Fusibacter</em>, <em>Pseudomonas</em>, <em>Ralstonia</em> and <em>Shewanella</em> were identified as the predominant bacterial strains in fresh shrimp samples, while lactic acid bacteria were found to enumerate substantially during shrimp deterioration. Pearson's correlation analysis was performed in this study, and significant strong correlations were found between the chemical degradative reactions and microbial growth, as well as the shrimp textural changes. The deteriorative mechanisms of Pacific whiteleg shrimp explored in this study can serve as the fundamental knowledge for developing advanced seafood preservation techniques or active packaging materials in the future.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102117"},"PeriodicalIF":4.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative analysis of the multi-element and pharmacological composition of Gastrodia elata blume from different geographic origins","authors":"Yujie Shang , Shunqiang Yang , Qinxiang Chang , Kaize Shen , Shaoning Chen , Zongsuo Liang","doi":"10.1016/j.jafr.2025.102087","DOIUrl":"10.1016/j.jafr.2025.102087","url":null,"abstract":"<div><div><em>Gastrodia elata</em> Blume tuber is widely recognized as both a food and medicine substance valued for its nutritional and therapeutic properties. However, environmental factors significantly influence its quality, medicinal efficacy, and overall value, making it crucial to comprehensively understand the quality of <em>G</em>. <em>elata</em> from different geographical origins. This study aims to discriminate <em>G</em>. <em>elata</em> specimens originating from three distinct geographical regions in China through comparative analysis of multi-element composition and pharmacological active ingredients. Using high-performance liquid chromatography (HPLC) and Inductively coupled plasma-Mass Spectrometry (ICP-MS), we quantified 17 elements and 6 active ingredients across the samples. Multivariate statistical methods, including cluster heatmap, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were employed to assess compositional differences and identify potential biomarkers. Our results showed significant variations in both the chemical and mineral composition of the samples. Multivariate statistics indicated that HBA, PE, PB, and Fe, K, Ca, Mn, P, Na, Cu, Mg, and B were the potential markers for discrimination of <em>G. elata</em> from Shaanxi, Yunnan, and Guizhou. These findings demonstrate the potential of the developed analytical strategy for the identification and discrimination of <em>G. elata</em> from different regions, which integrated pharmacological composition with multi-element as the potential markers.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102087"},"PeriodicalIF":4.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoyang Liang , Haitao Wang , Chuanjuan Wang , Junjie Qin , Xuefeng Qiu , Yongxing Wen , Jiandong Wang , Xurong Mei
{"title":"Biogas slurry topdressing enhances the comprehensive benefits of maize: New perspectives on grain filling process, yield, and endogenous microbiome","authors":"Xiaoyang Liang , Haitao Wang , Chuanjuan Wang , Junjie Qin , Xuefeng Qiu , Yongxing Wen , Jiandong Wang , Xurong Mei","doi":"10.1016/j.jafr.2025.102111","DOIUrl":"10.1016/j.jafr.2025.102111","url":null,"abstract":"<div><div>Finding new approaches to enhance the comprehensive effects of maize has been the persistent pursuit of scientific researchers. A three-year field experiment was conducted to elucidate the possible mechanisms or pathways of biogas slurry topdressing as replacement of chemical fertilizer (BSTR) affecting maize yield, quality, economic benefits, and endogenous microbiome. Compared with pure chemical fertilizer topdressing (CF), BSTR further increased post-tasseling dry matter (DM) accumulation, grain-filling characteristic parameters and yield components, with the increase amplitude gradually rising as the BSTR proportion increased. BSTR had greater potential to stimulate grain yield potential, with the pure biogas slurry topdressing treatment (100%BS) being the most representative, significantly increasing yield by 26.50 % and 14.76 % compared with the CF treatment in 2022 and 2023, respectively. The higher BSTR ratio contributed to higher net income (NP), with 100 %BS increasing NP by an average of 3867.72 RMB/ha over three years compared with CF. Furthermore, grain filling and dehydration directly affected yield components, while yield and NP were primarily subject to direct feedback regulation by treatment, years, and DM accumulation and redistribution. We also found that grain endophytic bacteria were more responsive to BSTR than endophytic fungal—BSTR treatment increased ACE and Chao1 indices of grain endophytic bacteria and further adjusted the relative abundances of endophytic bacterial phyla such as Proteobacteria, Actinobacteriota, and Bacteroidota, based on CF treatment. BSTR facilitated the conservation of water and fertilizer resources and the circular reuse of agricultural waste, with 100 %BS treatment being optimal for enhancing the comprehensive benefits of maize.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102111"},"PeriodicalIF":4.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zongkui Chen , Xiangyu Xu , Jiayue Wang , Wenqi Yuan , Xiaomeng Shen , William D. Batchelor , Zhiyuan Yang , Yongjian Sun , Jun Ma , Xiafei Li
{"title":"Nitrogen use efficiency in high soil-plant nitrogen pool: Fertilizer management optimization via the DNDC model in rice fields","authors":"Zongkui Chen , Xiangyu Xu , Jiayue Wang , Wenqi Yuan , Xiaomeng Shen , William D. Batchelor , Zhiyuan Yang , Yongjian Sun , Jun Ma , Xiafei Li","doi":"10.1016/j.jafr.2025.102108","DOIUrl":"10.1016/j.jafr.2025.102108","url":null,"abstract":"<div><div>Enhancing the soil-plant nitrogen (N) pool (SPNP) in paddies system can mitigate environmental N pollution. However, a larger SPNP does not inherently improve N use efficiency (NUE), strategies to enhance NUE under high SPNP remain poorly understood. This study employed the DeNitrification-DeComposition model to evaluate fertilizer practices: single chemical N (SCN), SCN combining with green manure (GM), straw incorporation (SI) or manure fertilizer (MF), aiming for high NUE under elevated SPNP paddies in paddy systems. Results indicated that plant and soil microbial N fixation, soil organic and inorganic N, NH<sub>3</sub> volatilization, and N leaching were key factors affecting both SPNP and NUE. Among the practices, SCN at 100–150 kg N ha<sup>−1</sup> minimized organic and inorganic N loss and increased soil and plant N fixation, but yielded the lowest SPNP and NUE. Combining GM with SCN at 50–100 kg N ha<sup>−1</sup> (GMN50-100) increased soil organic N by 67 % and soil microbial N fixation by 5 %, while reducing NH3 volatilization by 20–30 kg N ha<sup>−1</sup>. This significantly enhanced NUE under elevated SPNP. Similarly, SI combined with SCN (SIN) at 100–150 kg N ha<sup>−1</sup> or MF combined with SCN (MFN) at 50–100 kg N ha<sup>−1</sup> both increased soil organic N by over 20 % and reduced NH<sub>3</sub> volatilization. However, the SIN strategy resulted in lower SPNP but higher NUE, while the MFN treatment yielded the highest SPNP but comparatively lower NUE. Consequently, superior GMN50-100 proved the most effective approach for sustaining high NUE under high SPNP conditions.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102108"},"PeriodicalIF":4.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Seif , Hanan Y. Aati , Bin Wei , Mohamed A. Tantawy , Mohamed Sayed , Ziyu Guo , Sherien M. Bakry , Mahmoud Emam
{"title":"Resveratrol from grape pomace: Mitigation of lead-induced oxidative stress-related renal toxicity: In silico and In vivo studies","authors":"Mohamed Seif , Hanan Y. Aati , Bin Wei , Mohamed A. Tantawy , Mohamed Sayed , Ziyu Guo , Sherien M. Bakry , Mahmoud Emam","doi":"10.1016/j.jafr.2025.102080","DOIUrl":"10.1016/j.jafr.2025.102080","url":null,"abstract":"<div><div>Utilizing industrial agricultural waste is a beneficial approach that can reduce the negative environmental impact of agro-waste and valorize its potential for use in the food, health, and agricultural sectors. This study used grape pomace as a low-cost source of resveratrol (RES).The RES content in grape pomace extract was estimated as 12 μg/g using multiple-reaction monitoring (MRM) LC-MS2, and its fingerprint protons were confirmed by nuclear magnetic resonance spectroscopic analysis (<sup>1</sup>H NMR). Based on the network pharmacology and molecular docking results, RES appears to have potential activity in relieving nephrotoxicity by ameliorating oxidative stress and inflammation pathways. Consequently, the in-vivo study confirmed the nephroprotective effects of RES in lead acetate (PbAc)-exposed rats. PbAc-induced kidney damage was evidenced by the overproduction of reactive oxygen/nitrogen species, elevation of inflammation markers, induction of pathological signs in PbAc-exposed rats, and significant increases in serum urea and creatinine levels. However, co-treatment with RES together with PbAc relieved the oxidative stress and inflammation at the protein and mRNA levels, as well as improved renal functions in PbAc-exposed rats. The integrated biomarker response (IBR) illustrated that RES significantly mitigated the toxic effects of PbAc exposure, which considers RES a potential nephroprotective natural drug obtained from grape pomace.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102080"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaoxin Zhang , Xuehang Wang , Yuan Qi , Pengyuan Pan , Xiaoyi Li , Yue Leng , Ji Wang
{"title":"Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice","authors":"Yaoxin Zhang , Xuehang Wang , Yuan Qi , Pengyuan Pan , Xiaoyi Li , Yue Leng , Ji Wang","doi":"10.1016/j.jafr.2025.102079","DOIUrl":"10.1016/j.jafr.2025.102079","url":null,"abstract":"<div><div>Walnut peptide (WP) was chelated with calcium (Ca) to prepare WP-Ca chelate. The characterization, stability, and absorption properties of WP-Ca were investigated. Results showed that WP showed a calcium-binding capacity of 14.22 μg/mg, primarily through interactions with nitrogen and oxygen atoms. WP-Ca had good thermal stability and gastrointestinal digestive stability. In the flipped rat intestinal capsule model, the calcium absorption capacity of WP-Ca increased by approximately 3.75 times, and at the same time enhanced the calcium transport of the Caco-2 cell monolayer. Additionally, the results of animal experiments on calcium deficiency induced by a low-calcium diet indicated that WP-Ca (66.67 mg Ca/kg bw) supplementation effectively corrected calcium imbalance, significantly (<em>P</em> < 0.05) increased trabecular number and cortical thickness, and improved skeletal mechanical strength. Pearson's correlation analysis showed that the microbial genera of <em>Bacteroidete</em>, <em>Akkermansia, Allobaculum,</em> and <em>Oscillospira</em> were significantly (<em>P</em> < 0.05) associated with increased bone mass. Overall, these findings suggest that WP-Ca shows great potential in the development of new and highly efficient calcium supplements for osteoporosis prevention, nutritional fortification for special populations, and innovation in functional foods.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102079"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng Meng , Rui Zhang , Fang Ning , Ying Sun , Yinyin Feng , Xiaoyang He , Ran Han , Qinghua Liu , Huiqing Sun
{"title":"Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves","authors":"Meng Meng , Rui Zhang , Fang Ning , Ying Sun , Yinyin Feng , Xiaoyang He , Ran Han , Qinghua Liu , Huiqing Sun","doi":"10.1016/j.jafr.2025.102105","DOIUrl":"10.1016/j.jafr.2025.102105","url":null,"abstract":"<div><div><em>Psidium guajava</em> L. leaves contain various bioactive components have been utilized in traditional medicine for the treatment of diseases. A novel acidic polysaccharide (GLP) was extracted and purified from <em>Psidium guajava</em> L. leaves, an underutilized by-product of guava processing. GLP. The molecular weight of GLP was found to be 1.723 × 10<sup>3</sup> kDa and mainly consists of arabinose, galactose, galacturonic acid and rhamnose using high performance liquid chromatography, ion chromatography, methylation analysis and nuclear magnetic resonance techniques. Key glycosidic linkages such as →3)-β-D-Gal<em>p</em>-(1→, →3,4)-β-D-Gal<em>p</em>A-(1→, and →3)-α-L-Ara<em>f</em>-(1 → . were identified. In a retinoic acid-induced mouse model of osteoporosis, GLP administration significantly improved trabecular bone microarchitecture by increasing bone volume fraction, trabecular number, and thickness, while decreasing trabecular separation. Additionally, GLP markedly reduced serum levels of bone turnover markers (ALP, OC, TRACP) and pro-inflammatory cytokines (IL-6, IL-2, TNF-α). Furthermore, GLP modulated gut microbiota composition, enhancing the relative abundance of beneficial genera such as <em>Lachnospira</em>, <em>Oscillospiraceae</em>, and <em>Dubosiella</em>. These findings suggest that GLP not only modulates bone metabolism and inflammation but also may serve as an innovative natural therapeutic agent or functional food ingredient for the prevention and management of osteoporosis, highlighting the potential of valorizing guava leaf by-products in industrial applications.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102105"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integrating recursive feature selection with automated machine learning framework for global wheat price prediction","authors":"Prity Kumari , N. Harshith , Athula Ginige","doi":"10.1016/j.jafr.2025.102113","DOIUrl":"10.1016/j.jafr.2025.102113","url":null,"abstract":"<div><div>Wheat price forecasting is challenging due to the interplay between economic trends, environmental variability and unpredictable market conditions, as well as the scarcity of reliable data. This research presents an innovative method for predicting global wheat prices by combining Recursive Feature Elimination with Cross Validation (RFECV) and Bayesian Ridge Regression in an Automated Machine Learning (AutoML) framework. The inclusion of key input variables - Lag 1, Lag 2 and an outlier indicator, marks a significant improvement in capturing regular and extreme market events which have a strong impact on prices. A comprehensive evaluation was conducted using 32 years of monthly wheat price data (1990–2022) from the Federal Reserve Economic Database, comparing 35 machine learning models. . Based on test set performance, top five performing models <em>i.e.</em> Bayesian Ridge, Linear Regression, Least Angle Regression (Lars), Least Angle Regression with Cross-Validation (LarsCV) and Least Absolute Shrinkage and Selection Operator Least Angle Regression with Cross-Validation (Lasso Lars CV), were selected and further assessed across four train-test splits (80:20, 75:25, 70:30 and 65:35). The 80:20 split provided the most stable results, with Bayesian Ridge achieving the lowest RMSE of 12.26, outperforming the other models by 1.39% to 2.61% across all splits. These findings highlight the model’s generalizability and potential application in policy formulation, market regulation and strategic planning for global wheat trade.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102113"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miguel D. Ferro , Irene Caño-Carrillo , Bienvenida Gilbert-López , Alfonso Fernández-García , Maria João Cabrita , José M. Herrera , Maria F. Duarte
{"title":"Agricultural practices as promoters of oleocanthal and oleacein availability in virgin olive oils from three olive cultivars","authors":"Miguel D. Ferro , Irene Caño-Carrillo , Bienvenida Gilbert-López , Alfonso Fernández-García , Maria João Cabrita , José M. Herrera , Maria F. Duarte","doi":"10.1016/j.jafr.2025.102103","DOIUrl":"10.1016/j.jafr.2025.102103","url":null,"abstract":"<div><div>Virgin olive oil (VOO) is one of the greatest differentiating elements within the Mediterranean Diet, mainly due to its health-promoting properties associated with the presence of specific antioxidants—the phenolic compounds (PC). Among all PC in VOO, oleocanthal and oleacein are two of the most important, mainly due to their strong association with health benefits. The presence of these PC in VOO can be induced by many factors, such as agricultural management approaches. To evaluate the relevance of the agronomic practices as inducers of oleocanthal and oleacein in VOO, monovarietal VOO from three cultivars were produced —‘Cobrançosa’ (COB), ‘Arbequina’ (ARB), and ‘Galega vulgar’ (GV)—by a laboratory-scale extraction method, considering two distinct agronomic systems: organic and integrated farming systems. From the obtained VOO, oleocanthal and oleacein were quantified by HPLC-TOFMS. Significantly higher oleocanthal and oleacein concentrations were found in all organic VOO, with COB being the cultivar presenting the highest concentrations, with 561 and 268 mg/kg for oleocanthal, and 348 and 164 mg/kg for oleacein for organic and integrated VOO, respectively. In contrast, GV showed considerably lower values than COB and closer to ARB, with 110 and 0.17 mg/kg for oleocanthal, and 113 and 0.18 mg/kg for oleacein for organic and integrated farming systems, respectively. These results clearly show that both agronomic practices and cultivar can highly influence the chemical and nutritional properties of VOO, with organic farming potentiating the concentration of oleocanthal and oleacein. Of the studied cultivars, COB showed the highest concentration of the target compounds.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"22 ","pages":"Article 102103"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}