Journal of Agriculture and Food Research最新文献

{"title":"Proteomic profiling of pig placenta reveals key biomarkers linked to sow reproductive performance","authors":"Phurt Harnvoravongchai ,&nbsp;Matthew Phanchana ,&nbsp;Nutthida Pholmanee ,&nbsp;Boonyarut Ladda ,&nbsp;Thanyapit Thita ,&nbsp;Puey Ounjai ,&nbsp;Sittiruk Roytrakul ,&nbsp;Tavan Janvilisri","doi":"10.1016/j.jafr.2025.101858","DOIUrl":"10.1016/j.jafr.2025.101858","url":null,"abstract":"<div><div>Reproductive performance is a key factor in swine farming, yet the molecular mechanisms underlying critical reproductive traits remain poorly understood. This study utilized mass spectrometry-based proteomics to analyze protein expression profiles in pig placenta, categorizing samples by sow age, parity, and stillbirth incidence. A total of 8261 proteins were identified, with gene ontology analysis highlighting roles in proteolysis, transcriptional regulation, and immune response. Comparative analysis revealed 87 differentially expressed proteins (DEPs) between young and old sows, 115 DEPs between low and high parity sows, and 103 DEPs linked to stillbirth incidence. Notable proteins, such as laminin G2, methionine sulfoxide reductase B3, and FERM domain-containing 7, were significantly correlated with reproductive traits. Integrative analysis identified YEATS domain-containing 4 as a consistently downregulated protein across all traits, while nephrocystin 1 was linked to low birth weight and stillbirth incidence. Functional enrichment analysis underscored the role of cytoskeletal proteins and transporters in placental nutrient exchange and fetal development. This study expands the understanding of pig placental proteomics and identifies potential biomarkers for improving sow reproductive performance. Future studies will focus on validating these findings and translating them into practical applications for breeding selection and reproductive management in swine production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101858"},"PeriodicalIF":4.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and physical parameters supporting the top-rated product of Kapi-pla, a gastronomy of southern food, Thailand","authors":"Sunisa Siripongvutikorn,&nbsp;Kanyamanee Pumethakul,&nbsp;Jinattita thantrirat,&nbsp;Nualpun Sirinupong,&nbsp;Worrapanit Chansuwan,&nbsp;Worapong Usawakesmanee,&nbsp;Chanonkarn Rujirapong","doi":"10.1016/j.jafr.2025.101856","DOIUrl":"10.1016/j.jafr.2025.101856","url":null,"abstract":"<div><div><strong>“</strong>The Lost Taste Project”, Kapi-pla, fermented fish from Pak Phanang (Nakhon Si Thammarat) was declared the product champion based on committee judgment and popular vote without scientific information record even though it is regularly consumed in the south of Thailand. This present work aims to disclose the traditional process and indicate typical characteristics of Kapi-pla for preparing standard regulation and supporting next approval projects like southern gastro-democracy shortly. An insight interview indicated that each producer used his or her own ancestors’ guidelines and available fish following zero waste concept. Interestingly, the process requires a specific range of fish quality, namely soft texture and swollen sign before use. Kapi-pla showed a brown-gray of thick-clump texture with bone, spine and scale of the used fish. Amino acids composition profile determined by HPLC were classified as bitter-salty-sour (umami), sulphurous, bitter-salty-sweet, sweet-bitter and sweet taste while fatty acids profile analyzed by GC were palmitic acid, stearic acid, oleic acid and palmitoleic acid which were linked to taste sensation of Kapi-pla from each area. Results indicated that Kapi-pla from brackish-marine water fish (B-MF) (Pak Phanang, Nakhon Si Thammarat) seemed to contain more sweet amino acids including glycine, lower or even the lowest undesired taste as threonine and lysine, and less allergic precursor (histidine). In addition, it contained higher desired fatty acid composition (EPA and DHA) in comparison to others. Chemical and physical parameters obtained from this experiment were the first documentation supporting and relating to public consumer tests. All scientific parameters are proceeding to use for Kapi-pla standard regulation issued by Thai FDA later. Additionally, Thai government has been selected and appointed Kapi-pla as one of southern gastro-democracy items and planned to create several <em>de novo</em> menus using either alone or as an ingredient in other menus for welcome visitors and exported products further.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101856"},"PeriodicalIF":4.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial potential of cell-free supernatants from Liquorilactobacillus nagelii and Lactococcus garvieae isolated from the gastrointestinal tract of Asian green mussel (Perna viridis) against foodborne pathogenic and spoilage bacteria","authors":"Jyoti Kumari ,&nbsp;Avtar Singh ,&nbsp;Nuntiya Pahumunto ,&nbsp;Soottawat Benjakul ,&nbsp;Jirayu Buatong","doi":"10.1016/j.jafr.2025.101855","DOIUrl":"10.1016/j.jafr.2025.101855","url":null,"abstract":"<div><div>This study investigated the antibacterial activity of cell-free supernatants (CFS) from 33 lactic acid bacteria (LAB) isolated from the gastrointestinal (GI) tract of Asian green mussel (<em>Perna viridis</em>) against <em>Vibrio parahaemolyticus</em>, <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em> and <em>Shewanella putrefaciens</em> using the agar well diffusion method. Out of 33 isolates, three (9.1 % of the total; MG1, MG2 and MG5) exhibited strong activity against <em>V. parahaemolyticus</em>, with an inhibition zone in the range of 10.00–13.20 mm. The freeze-dried CFS of strains MG1, MG2 and MG5 exhibited inhibitory effects against <em>V. parahaemolyticus</em>, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranging from 4 to 16 mg/mL, 8–16 mg/mL and 8–64 mg/mL, respectively. Scanning electron microscopic images of <em>V. parahaemolyticus</em> cells treated with freeze-dried CFS of strains MG1, MG2 and MG5 at 4 × MIC showed cell damage as indicated by pore formation on the cell surface along with the absence of flagella, potentially caused by a synergistic effect of the major bioactive components in LAB supernatant. Freeze-dried CFS of strain MG2 at MIC (8 mg/mL) was most effective at inhibiting motility (swimming and swarming) and biofilm formation of <em>V. parahaemolyticus</em>, with 100 % and 98.5 % inhibition, respectively. Based on MALDI-TOF MS and the 16s rRNA gene sequence, strains MG1 and MG2 were identified as <em>Liquorilactobacillus nagelii</em>, while strain MG5 was identified as <em>Lactococcus garvieae</em>. Analysis of freeze-dried CFS from strain MG2 using LC-Q-TOF-MS revealed that 3b,6a-dihydroxy-alpha-ionol 9-[apiosyl-(1-&gt;6)-glucoside] and citric acid were the major components detected in positive and negative mode, respectively. This is the first report of strong antibacterial activity exhibited by antimicrobial agents from <em>L. nagelii</em> isolated from Asian green mussels. These findings suggest that antimicrobial agents derived from LAB in Asian green mussels may serve as potential biocontrol strategies for managing foodborne pathogens and spoilage bacteria.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101855"},"PeriodicalIF":4.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Puerarin, a key bioactive ingredient of Radix Puerariae, inhibits oxidative stress and protects against homocysteine-induced cardiac injury via PI3K/Akt/Nrf2/HO-1 signaling","authors":"Shuang Jiang,&nbsp;Cong Li,&nbsp;Yanxing Han,&nbsp;Jiandong Jiang,&nbsp;Yuhong Wang","doi":"10.1016/j.jafr.2025.101854","DOIUrl":"10.1016/j.jafr.2025.101854","url":null,"abstract":"<div><div>Elevated plasma homocysteine (Hcy) levels are a major contributor to the risk factor for the developing cardiovascular diseases (CVDs). This study explored the protective efficacy of puerarin (Pue), a key bioactive ingredient derived from Radix Puerariae, against cardiac damage induced by Hcy, along with its underlying molecular mechanisms. Using H9c2 cardiomyocytes and a guinea pig model of hyperhomocysteinemia (HHcy), we demonstrated that Pue attenuated Hcy-induced cardiotoxicity, oxidative stress, mitochondrial dysfunction and apoptosis. The cardioprotective effects of Pue were exerted by activating the PI3K/Akt/Nrf2/HO-1 signaling axis. <em>In vitro</em>, Pue pretreatment markedly enhanced cell viability, diminished the release of lactate dehydrogenase (LDH), lowered reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and elevated superoxide dismutase (SOD) activities in Hcy-injured H9c2 cardiomyocytes. Furthermore, Pue upregulated Nrf2 and HO-1 expression, two key regulators of the antioxidant response, and alleviated Hcy-induced mitochondrial dysfunction and apoptosis. These beneficial effects were abrogated by the HO-1 inhibitor Znpp and the PI3K inhibitor LY294002, indicating the contribution of the PI3K/Akt/Nrf2/HO-1 axis. <em>In vivo</em>, Pue treatment significantly reduced serum Hcy levels, decreased serum biomarkers of myocardial injury, lowered MDA levels, and increased SOD activities in HHcy guinea pigs. Additionally, Pue improved cardiac remodeling and attenuated myocardial apoptosis, accompanied by upregulation of Nrf2 and HO-1 expression and an increased p-Akt/Akt ratio within myocardial tissues. Overall, these findings suggest that Pue protects against Hcy-induced cardiac injury via the PI3K/Akt/Nrf2/HO-1 signaling axis, offering a potential therapeutic approach for the prevention and management of CVDs associated with elevated Hcy levels.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101854"},"PeriodicalIF":4.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of key challenges to implementation of FEFO in perishable food supply chain","authors":"Jayakrishna Kandasamy ,&nbsp;K.E.K. Vimal ,&nbsp;Aditya Pratap Singh ,&nbsp;Aaryan Magnani ,&nbsp;Ameya Gokhale ,&nbsp;Sandeep Jagtap","doi":"10.1016/j.jafr.2025.101848","DOIUrl":"10.1016/j.jafr.2025.101848","url":null,"abstract":"<div><div>Implementing FEFO practices has become essential for organizations globally to minimize spoilage, enhance inventory turnover, and ensure compliance with health and safety standards. To aid stakeholders in effectively adopting FEFO, it is crucial to identify and address the challenges involved in its implementation. Through an extensive literature review using the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) methodology and insights from industry experts, this study identifies thirteen core challenges that hinder FEFO adoption. PRISMA methodology was used to systematically organize the existing literature for the purpose of this study. Using tools like Decision Making Trial and Evaluation Laboratory (DEMATEL) and Total Interpretive Structural Modelling (TISM), the challenges were examined and ranked according to their interdependencies, providing insights into the cause-effect relationships among them. After applying DEMATEL, an alpha threshold value of 0.368 revealed that challenges in effective storage management are the primary barrier in implementing FEFO practices. With level partitioning, this challenge emerged as the most significant, forming the foundation for a roadmap designed to assist stakeholders. The findings from this study offer managers actionable insights for implementing effective FEFO techniques within their organizations. The study's novelty lies in its combination of DEMATEL and TISM methodologies, along with a roadmap that highlights strategic and policy-focused recommendations to support efficient FEFO adoption and the systematic study of challenges preventing effective FEFO adoption. This paper aids implementation of FEFO for better inventory control and management, reduced wastage and greater efficiency. The paper also effectively outlines and analyses the order of importance of challenges in FEFO implementation and their interdependence.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101848"},"PeriodicalIF":4.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating soda-lime adsorption and microcontroller-based system for the dynamic control of CO2 in a fresh produce storage box under varying temperature","authors":"Yogesh B. Kalnar ,&nbsp;Akshay D. Sonawane ,&nbsp;Cornelia Weltzien ,&nbsp;Pramod V. Mahajan","doi":"10.1016/j.jafr.2025.101852","DOIUrl":"10.1016/j.jafr.2025.101852","url":null,"abstract":"<div><div>Effective storage of fresh produce requires precise control of temperature, O₂, CO₂, and relative humidity. The storage system with an air exchange to control O₂ has already been developed. In this study, a soda-lime reactor was introduced into the developed storage system for CO₂ control. Initially, the kinetics of CO₂ adsorption by soda-lime was studied at different temperatures (5, 10 and 20 °C) and relative humidity (&gt;85 %). Then, the new system was integrated into a storage box containing 16 kg of broccoli and the performance evaluation was conducted under varying temperatures from 5 to 23 °C over a 120-h storage period. The analysis of soda-lime adsorption using the Weibull function showed an adsorption capacity of 267 L kg⁻¹. Adsorption was triggered at relative humidity above 80 %, highlighting its suitability for high-humidity conditions in fresh produce storage. While temperature affected the adsorption rate of soda-lime, it did not impact the overall capacity. During the entire storage period, CO₂ and O₂ concentrations inside the box were successfully maintained at 4 ± 2 % and 2 ± 1 %, respectively, even under varying temperatures. This approach highlights the potential of air exchange and soda-lime-based system as an alternative solution for the indirect control of O₂ as well as CO₂, using a simple temperature sensor and mathematical model.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101852"},"PeriodicalIF":4.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of pre- and post-harvesting techniques on phenolics, antioxidant activities and key enzyme inhibitions of commercially available ready-to-drink teas","authors":"Chanakan Khemthong,&nbsp;Sirinapa Thangsiri,&nbsp;Wimonphan Chathiran,&nbsp;Woorawee Inthachat,&nbsp;Chaowanee Chupeerach,&nbsp;Piya Temviriyanukul,&nbsp;Nattira On–Nom,&nbsp;Warangkana Srichamnong,&nbsp;Uthaiwan Suttisansanee","doi":"10.1016/j.jafr.2025.101853","DOIUrl":"10.1016/j.jafr.2025.101853","url":null,"abstract":"<div><div>Tea, the world's most popular beverage, is prepared using different pre- and post-harvesting techniques, leading to its unique sensory characteristics and bioactive ingredients. However, knowledge about the impact of these processing methods on the phenolic compositions and health properties of commercially available ready-to-drink teas is limited. To fill this research lacuna, the phenolic compositions, antioxidant potentials, and inhibitory activities against the key enzymes relevant to non-communicable diseases including hyperlipidemia (lipase), type II diabetes (α-amylase, α-glucosidase, and dipeptidyl peptidase-IV), and Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase, and β-secretase) of eleven commercially available ready-to-drink teas were investigated. The results indicated that pre-harvest shading led to higher contents of catechin, epicatechin gallate, chlorogenic acid, and 3,4-dihydroxybenzoic acid, while fermentation significantly increased rutin content, which, in turn, led to higher total phenolic contents. Antioxidants in shaded tea tended to follow a hydrogen atom transfer (HAT)-based mechanism, while the single electron transfer (SET)-based mechanism was a preferable reaction pathway for antioxidants in fermented teas. The ability to inhibit key enzymes was more pronounced in fermented teas than in shaded tea, potentially attributed to the biological activities of certain phenolics, either individually or working together as effective inhibitors. The addition of flavors (honey lemon, kyoho grape, watermelon, and roasted rice) led to higher contents of catechins than the original green tea (without added flavor), while the contents of other general phenolics varied, leading to similar or higher TPCs. Antioxidant potentials and enzyme inhibitions varied for different ready-to-drink tea varieties. The knowledge from this research will benefit the development of ready-to-drink teas with particular phenolics and health-related properties.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101853"},"PeriodicalIF":4.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jerusalem artichoke extracts regulate the gene expression of key enzymes involved in fatty acid biosynthesis","authors":"Soo Jin Lee ,&nbsp;Woo-Cheol Shin ,&nbsp;Sangmin Ju ,&nbsp;Mi-Ri Gwon ,&nbsp;Jae-Hwa Lee ,&nbsp;Young-Ran Yoon ,&nbsp;Stuart K. Calderwood ,&nbsp;Dae Young Lee ,&nbsp;Heeyoun Bunch","doi":"10.1016/j.jafr.2025.101819","DOIUrl":"10.1016/j.jafr.2025.101819","url":null,"abstract":"<div><div>Jerusalem artichoke (JA) is a traditional remedy for alleviating symptoms of diabetes. In fact, the suppressive effects of JA on blood sugar have been reported in multiple studies since 1934. Recent studies have indicated that type II diabetes is often caused by insulin resistance rather than insulin reduction and that increased blood and interstitial fatty acid levels contribute to insulin resistance and the development of diabetes. However, whether JA affects human lipogenesis has not been studied. Here, we elucidated the effects of JA on the expression of two key enzymes involved in fatty acid biosynthesis, fatty acid synthase (<em>FASN</em>) and acetyl-CoA carboxylase (<em>ACACA</em>), using three human neuroblastoma and colon and liver cancer cell lines. Caffeine and ICRF193, a catalytic inhibitor of topoisomerase II (TOP2), were included as positive controls, and JA was extracted into water- or dimethyl sulfoxide-soluble components, termed H-JA and D-JA. Metabolomics analyses using gas chromatography-time of flight/mass spectrometry and in-silico analyses showed differentially enriched chemical compounds in H-JA and D-JA, suggesting their distinctive bioactivities including fatty acid metabolism. D-JA significantly reduced the expression of <em>FASN</em> and <em>ACACA</em> at the mRNA and protein levels. D-JA-treated cells exhibited altered TOP2 levels and <em>FASN</em>/<em>ACACA</em> expression appeared to be controlled by TOP2 activity and levels. Taken together, our study revealed a novel effect of JA extracts on inhibiting the expression of the key enzymes involved in the fatty acid biosynthesis and suggested the potential of JA as a natural medicinal agent to control lipogenesis in humans.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101819"},"PeriodicalIF":4.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dry fruit image classification using stacking ensemble model","authors":"Maheen Islam ,&nbsp;Mujahidul Islam ,&nbsp;Alfe Suny ,&nbsp;Abdullah Al Rafi ,&nbsp;Abdullahi Chowdhury ,&nbsp;Mohammad Manzurul Islam ,&nbsp;Saleh Masum ,&nbsp;Md Sawkat Ali ,&nbsp;Taskeed Jabid ,&nbsp;Md Mostofa Kamal Rasel","doi":"10.1016/j.jafr.2025.101850","DOIUrl":"10.1016/j.jafr.2025.101850","url":null,"abstract":"<div><div>Precise and efficient classification of dry fruit images is critical for enhancing quality control, efficiency, and safety in the agricultural and food industries. This study presents a CNN-based classification model developed to analyze a diverse dataset of dry fruit images. The dataset comprises 11,520 high-resolution images representing four varieties (raisins, cashews, almonds, and figs), each divided into three groups, resulting in twelve distinct classes. The proposed multi-step methodology covers data collection, preprocessing, augmentation, and model training. A wide range of image conditions, including variations in fruit types, shapes, colors, and lighting, facilitated comprehensive experimentation and validation using performance indicators such as accuracy. A stacking ensemble, integrating predictions from multiple models (e.g., VGG16, VGG19, ResNet50, MobileNetV1, Mo- bileNetV2, SqueezeNet, and ShuffleNet), achieved a test accuracy of 98.32 %, surpassing individual base model performances (MobileNetV2: 90.33 %, Mo- bileNetV1: 93.68 %, SqueezeNet: 96.98 %, and ShuffleNetV2: 97.79 %). These findings underscore the model’s potential for real-time applications in qual-ity control, automated processing, nutritional research, and other domains, while also delineating the key components required for accurate classification.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101850"},"PeriodicalIF":4.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive effects of drought and high night temperature on physiology and yield components of cowpea (Vigna unguiculata (L.) Walp.)","authors":"Alekhya Chakravaram ,&nbsp;Lekshmy V. Sankarapillai ,&nbsp;Sujan Poudel,&nbsp;Raju Bheemanahalli","doi":"10.1016/j.jafr.2025.101844","DOIUrl":"10.1016/j.jafr.2025.101844","url":null,"abstract":"<div><div>Cowpea is a nutrient-rich vegetable legume with significant potential to address protein-calorie malnutrition. However, exposure to drought (DS) and warmer night temperatures (HNT) substantially threaten crop production, including legumes. Several studies have examined the effects of DS, but there is limited information on the combined impact of DS and HNT during the reproductive growth stage of cowpeas. This study quantified the effect of individual DS or HNT and combination on physiological and yield-related traits. At the reproductive stage, three genetically diverse cowpea genotypes were exposed to four growing conditions: (i) control (CNT, 24°C night temperature, full irrigation), (ii) drought (DS, 24°C night temperature, 40% irrigation of CNT), (iii) high night temperature (HNT, 28°C night temperature, full irrigation), and (iv) DS+HNT (28°C night temperature + 40% irrigation of CNT), with a common day temperature (32°C). A 3.2°C nighttime rise under DS reduced stomatal conductance by 91.5%, photosystem efficiency (17.4%), resulted in a lower pod weight (64.5%), seeds per pod (14%), and seed count (66%). Individual stressors notably impacted seed number and yield, with DS resulting in reductions exceeding 30%, followed by HNT at 23% compared to CNT. Overall seed yield decreased by 63% and seed protein by 25.1% under DS+HNT. Among genotypes, Episelect.1 showed greater tolerance to combined stress, while UCR 369 performed better under HNT. The stress impact on cowpea performance ranked as DS+HNT &gt; DS &gt; HNT, highlighting the need for further research to understand molecular mechanisms that cause different stress responses during reproductive and grain-filling phases.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101844"},"PeriodicalIF":4.8,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}