Journal of Agriculture and Food Research最新文献

{"title":"Food phytochemicals as epigenetic modulators in diabetes: A systematic review","authors":"Fahrul Nurkolis ,&nbsp;Nurpudji Astuti Taslim ,&nbsp;Rony Abdi Syahputra ,&nbsp;Annette d’Arqom ,&nbsp;Raymond Rubianto Tjandrawinata ,&nbsp;Abdul Khairul Rizki Purba ,&nbsp;Arifa Mustika","doi":"10.1016/j.jafr.2025.101873","DOIUrl":"10.1016/j.jafr.2025.101873","url":null,"abstract":"<div><div>Diabetes mellitus, a metabolic disorder affecting over 537 million adults globally, necessitates therapeutic approaches that extend beyond conventional glycemic control. Emerging evidence highlights the role of food-derived phytochemicals such as resveratrol, curcumin, and sulforaphane as epigenetic modulators capable of influencing gene expression through DNA methylation, histone modifications, and non-coding RNA regulation. This systematic review synthesizes preclinical and clinical studies to elucidate the mechanisms by which these bioactive compounds modulate diabetes-related epigenetic alterations. Findings indicate that phytochemicals may improve insulin sensitivity, enhance β-cell survival, and mitigate inflammation via epigenetic reprogramming. However, challenges such as bioavailability, interindividual variability, and translational feasibility persist. Addressing these barriers through advanced delivery systems, precision nutrition, and synergistic therapeutic strategies may enhance the clinical utility of phytochemicals in diabetes management. This review underscores the untapped potential of epigenetically active food compounds in redefining diabetes therapy, advocating for further research into their mechanistic pathways and clinical applications.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101873"},"PeriodicalIF":4.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into the antimicrobial activity of plant-based immunomodulators: A narrative review","authors":"Yuandani ,&nbsp;Ibrahim Jantan ,&nbsp;Ade Sri Rohani ,&nbsp;Abdi Wira Septama ,&nbsp;Nur Aini Khairunnisa ,&nbsp;Halimah Raina Nasution ,&nbsp;Diding Pradita ,&nbsp;Rony Abdi Syahputra ,&nbsp;Fadli Mubaroq Nasution ,&nbsp;Wahrianto ,&nbsp;Madeline Hana Tasya Siburian","doi":"10.1016/j.jafr.2025.101872","DOIUrl":"10.1016/j.jafr.2025.101872","url":null,"abstract":"<div><div>Immunomodulators have been used in combination with other therapeutic modalities as a prophylaxis to prevent or treat various immune-related diseases including infectious diseases. They have been investigated as possible alternative anti-infectives which are less toxic and inexpensive, and less prone to microbial resistance than the contemporary antimicrobials. The mechanisms of antimicrobial activity of these immunomodulators may involve stimulation of secretion of cytokines, enhancing phagocytosis, and producing antimicrobial peptides to effectively assist the immune system to combat infections. In this review, the antimicrobial effects and mechanisms of various plant-based immunomodulators through modulation of immune response were critically analyzed and discussed. Various studies have provided quantitative data on the effectiveness of these immunomodulators including lectins, polysaccharides, alkaloids, flavonoid glycosides, triterpene glycosides, and polyphenols in enhancing the body's defense mechanisms against infections. Among these, lectins and polysaccharides have been much investigated. The compounds were able to trigger the release of soluble mediators that assist in coordinating the immune system against pathogens and modulate the activity of immune cells against bacteria via phagocytosis. However, there is limited investigations on their efficacy as possible alternative anti-infectives. Most studies have been carried out experimentally to elucidate the specific mechanisms underlying the antimicrobial effects of these immunomodulators. Future research on the antimicrobial effects of plant-based immunomodulators in humans is essential to elucidate their mechanisms of action within the human immune system. Furthermore, thorough studies are necessary to evaluate their safety and efficacy for clinical use, ensuring their potential as valuable tools in the fight against infections.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101872"},"PeriodicalIF":4.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of silver nanoparticles from Pseudophegopteris microstegia (Hook.) Ching and their biological activities against tomato gray mold","authors":"Iraj Mushtaq ,&nbsp;Sadaf Kayani ,&nbsp;Tijen Demiral Sert ,&nbsp;Qaisar Abbas Bhatti ,&nbsp;Muhammad Zeeshan Qureshi ,&nbsp;Hu Shubao ,&nbsp;Kamran Iqbal Shinwari","doi":"10.1016/j.jafr.2025.101871","DOIUrl":"10.1016/j.jafr.2025.101871","url":null,"abstract":"","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101871"},"PeriodicalIF":4.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and functional characterization of melissa essential oil fortified k-carrageenan-based active packaging films","authors":"Saurabh Bhatia ,&nbsp;Muhammad Jawad ,&nbsp;Ahmed Al-Harrasi ,&nbsp;Yasir Abbas Shah ,&nbsp;Talha Shireen Khan ,&nbsp;Esra Koca ,&nbsp;Levent Yurdaer Aydemir ,&nbsp;Dinu Thomas Thekkuden ,&nbsp;Faisal Imam ,&nbsp;Naif Al-Harbi","doi":"10.1016/j.jafr.2025.101870","DOIUrl":"10.1016/j.jafr.2025.101870","url":null,"abstract":"<div><div>The study examined the use of Melissa essential oil (MEO) in kappa carrageenan (KC) based films as a form of active packaging for potential applications in food packaging. Concentrations of MEO used were 0.1 %, 0.5 %, 1 %, and 1.5 % (v/v) to observe changes in the biopolymer characteristics. The films were evaluated for their appearance, color, antioxidant properties, and mechanical characteristics, including tensile strength (TS) and elongation at break (EAB). The findings indicate that the increase in MEO concentration resulted in a considerable decrease in the TS and moisture content of the films. In addition, an increase in EAB and water vapor permeability (WVP) was observed due to the increase in MEO concentration. The results of color analysis show that higher MEO levels made the films opaque with increasing yellowness (b∗). Moreover, antioxidant assays revealed that increasing the concentration of MEO significantly improved the films' scavenging activity against free radicals (DPPH) and cationic radicals (ABTS⁺). Additionally, characterization techniques such as FTIR, XRD, SEM, and thermal assessments (TGA, DSC and DTG) were also performed. Microstructural analysis revealed that a higher MEO concentration led to a few particles in the film structure. Overall, the results indicate a relatively consistent microstructure. The thermal analysis revealed that the increase in MEO concentration leads to a noticeable improvement in the film's thermal stability.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101870"},"PeriodicalIF":4.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards sustainable management of cassava mosaic disease: The impact of awareness campaigns in Benin","authors":"Dèwanou Kant David Ahoya ,&nbsp;Jacob Afouda Yabi ,&nbsp;Jerome Anani Houngue ,&nbsp;Serge Sètondji Houédjissin ,&nbsp;Martine Zandjanakou-Tachin ,&nbsp;Ettien Antoine Adjei ,&nbsp;Eveline Marie Fulbert Windinmi Sawadogo-Compaore ,&nbsp;Justin Simon Pita ,&nbsp;Corneille Ahanhanzo","doi":"10.1016/j.jafr.2025.101827","DOIUrl":"10.1016/j.jafr.2025.101827","url":null,"abstract":"<div><div>Transboundary diseases, such as cassava mosaic disease (CMD), represent a significant risk to food security and the livelihoods of millions of households in sub-Saharan Africa. To address this issue, awareness campaigns have been conducted targeting farmers and stakeholders within the cassava secor. The objective of this study is to quantify the impact of these awareness campaigns on the knowledge of CMD, the adoption of management practices, and the incidence of the disease in cassava fields. A random sample of 305 farmers and 77 cassava fields in Benin was selected for data collection. To account for potential selection bias associated with observable characteristics, we applied Propensity Score Matching (PSM). The results indicate that farmers who participated in the training demonstrated significantly higher levels of CMD knowledge and were more likely to adopt a greater number of management practices, which ultimately led to a reduction in the prevalence of the disease in their fields. However, CMD symptoms were still prevalent in the majority (61,04) of cassava farms, regardless of participation in the campaigns, due to the lack of healthy planting material and the abundance of whiteflies. These findings suggest that awareness campaigns can significantly improve farmers' knowledge and encourage behavioural changes in the identification and adoption of sustainable CMD management practices. It also shows the need to provide famers with healthy cuttings for more effective disease management.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101827"},"PeriodicalIF":4.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative nutrient analysis of multiple Saskatchewan wild rice (Zizania palustris L.) populations and commercially available grains","authors":"Sunny Yang ,&nbsp;Asket Kaur ,&nbsp;Serena Page ,&nbsp;Yongfeng Ai ,&nbsp;Sahar Zamani ,&nbsp;Mark Smith ,&nbsp;Blaine Chartrand ,&nbsp;Pankaj Bhowmik ,&nbsp;Timothy F. Sharbel ,&nbsp;Albert Vandenberg ,&nbsp;Haixia Zhang","doi":"10.1016/j.jafr.2025.101866","DOIUrl":"10.1016/j.jafr.2025.101866","url":null,"abstract":"<div><div>Wild rice (<em>Zizania</em> spp.) is an aquatic grass grown in North America and East Asia. It is an ancient food source for Indigenous Peoples and is now commercially available as a specialty grain. Despite interest in its nutritional profile, a systematic investigation is lacking. In this work, a thorough analysis of the nutrient components in fresh and processed wild rice from northern Saskatchewan was conducted. This covered macronutrients (proteins, starch and lipids), micronutrients (vitamin Bs and minerals), phenolic compounds and their antioxidant potential. Compared to selected cultivated rice and wheat grains, wild rice has higher protein content (9.8–12.1 %), similar levels of starch (73.7–76.5 %), and lower amounts of lipid (around 1 %) with a significantly higher proportion of omega-3 fatty acids (50–57 %) than rice (33–40 %). Compared to wheat, wild rice has twice the level of total vitamin B (77.1–107.2 μg/g), and three times the concentration of soluble phenolic compounds (87.6–111.7 μg/g). Strong antioxidant potential was also observed in wild rice (2.6–4.9 mg/g, trolox equivalent). Wild rice is a good source of minerals including potassium (1963–3031 μg/g), sulfur (1385–1757 μg/g), phosphorous (2215–3667 μg/g), magnesium (662–969 μg/g), and calcium (73–110 μg/g). The results showed that wild rice is a nutrition rich grain well suited for a healthy diet.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101866"},"PeriodicalIF":4.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-pressure processing of Porphyra sp.: A sustainable approach for enhanced protein and phycobiliprotein extraction and nutritional quality","authors":"Sukan Braspaiboon,&nbsp;Thunnop Laokuldilok","doi":"10.1016/j.jafr.2025.101868","DOIUrl":"10.1016/j.jafr.2025.101868","url":null,"abstract":"<div><div>High-pressure processing (HPP), a non-thermal technology, has gained attention in the food industry for its environmental sustainability and versatility in preservation and extraction. This study aimed to optimize HPP conditions to enhance the extraction of proteins and phycobiliproteins (PBP) from red algae (<em>Porphyra</em> sp.), highlighting their potential as sustainable bioresources. A Box-Behnken design was employed to optimize three key factors: pressure (100–600 MPa), time (10–30 min), and pH (4–10). The optimal conditions for maximizing nitrogen recovery and PBP content were 600 MPa, 28 min, and pH 7.8. Under these conditions, nitrogen recovery and PBP content reached 31 % and 1.57 g/100 g, respectively. Scanning electron microscopy (SEM) revealed that HPP-induced cell disruption led to significantly higher nitrogen recovery (31 %) compared to the alkaline method (27 %). The denaturation temperature of protein extracted using HPP was similar to that of untreated <em>Porphyra</em> sp. (151–154 °C) but significantly higher than that of alkaline-extracted protein (138 °C). Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated that HPP reduced protein denaturation compared to the alkaline method, preserving the amide I (∼1650 cm⁻¹) and II (∼1550 cm⁻¹) peaks characteristic of <em>Porphyra</em> sp. Nutritionally, HPP improved protein digestibility, increased the essential amino acid content of threonine, and enhanced both the amino acid score and the protein digestibility-corrected amino acid score (PDCAAS). These findings suggest that HPP is a promising green technology for producing cost-effective alternative proteins with superior functional and nutritional properties. Moreover, it promotes the sustainable utilization of marine resources, aligning with both environmental and economic goals.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101868"},"PeriodicalIF":4.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin evaluation and integrated management strategies to minimize toxin contamination in maize and peanuts","authors":"Muthusamy Karthikeyan ,&nbsp;Shanmuga Priya Dhanabalan ,&nbsp;Bavish Shanmugavel ,&nbsp;Shajith Basha Jaffer ,&nbsp;Subbaiyan Marimuthu ,&nbsp;Krishnan Radhika ,&nbsp;Elakkiya Nagappan ,&nbsp;Iruthayasamy Johnson ,&nbsp;Sambasivam Periyannan","doi":"10.1016/j.jafr.2025.101809","DOIUrl":"10.1016/j.jafr.2025.101809","url":null,"abstract":"<div><div>Commercial varieties of maize and peanuts are highly prone to <em>Aspergillus flavus</em> infection and aflatoxin contamination at pre and postharvest stages. In a screening of 600 samples each of maize and peanuts from Tamil Nadu, India, for the mycotoxin aflatoxin B1 (AFB1), 28 and 32.67 % of the maize and peanut samples tested positive, where 6 and 9.5 % of both pre and postharvest maize and peanut samples exceeded the minimum threshold level of 20 μg/kg. The highest level of AFB1 was recorded as 147.23 and 162.72 μg/kg in maize and peanut and in postharvest samples. Additionally, a high number of positive samples in postharvest and Rabi season crops indicates the influence of moisture on <em>A. flavus</em> proliferation and toxin production. Effective preharvest management practices include the integrated use of biocontrol agents such as <em>Trichoderma viride</em> and <em>Bacillus subtilis</em>, while postharvest measures involve drying of harvest produce (with moisture content below 9 %), treatment with <em>Andrographis paniculata</em> extracts and storage in jute bags. Further, our findings emphasize the integration of pre and postharvest management strategies to mitigate <em>A. flavus</em> infection and aflatoxin contamination in maize and peanuts, thereby ensuring food security for the growing human population.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101809"},"PeriodicalIF":4.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chicken meat hydrolysate improves acetaminophen-induced liver injury by alleviating oxidative stress via modulation in Keap1/Nrf2/HO-1 signaling in BALB/c mice","authors":"Vipul Wayal ,&nbsp;Zhuo-En Tsai ,&nbsp;Yu-Hsuan Lin ,&nbsp;Yi-Hsuan Lai ,&nbsp;Shulhn-Der Wang ,&nbsp;Chang-Chi Hsieh","doi":"10.1016/j.jafr.2025.101863","DOIUrl":"10.1016/j.jafr.2025.101863","url":null,"abstract":"<div><div>Acetaminophen (APAP)-induced hepatotoxicity is a major cause of acute liver injury, primarily due to excessive oxidative stress and inflammation. Chicken meat hydrolysate (CMH) is recognized for their diverse bioactivities, including potent antioxidant and anti-inflammatory properties, making them potential candidates for hepatoprotection. This <em>in vivo</em> study investigates the hepatoprotective potential and underlying mechanisms of CMH against APAP-induced liver injury in BALB/c mice. Enzymatically hydrolyzed CMH was administered via oral gavage at low (50 mg/kg/day) and high (200 mg/kg/day) doses, carnosine (50 mg/kg/day) as a reference standard, for seven consecutive days. On the eighth day, all groups except the Naïve group received APAP (300 mg/kg, i. p.), and mice were sacrificed 24 h later for further evaluation. Our findings demonstrate that one-week pre-treatment with CMH effectively preserved liver morphology, reduced serum liver injury markers including aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase, and mitigated histopathological alterations. Furthermore, it suppressed inflammatory proteins and cytokine expression, including C-reactive protein, interleukin-6, and monocyte chemoattractant protein-1, while downregulating hepatic 4-hydroxynonenal levels, a marker of lipid peroxidation. Mechanistically, CMH enhanced antioxidant defenses by activating Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway and preventing Keap1-mediated Nrf2 degradation. This resulted in increased levels of key antioxidant enzymes, including superoxide dismutase and glutathione peroxidase, as well as intracellular glutathione, promoting hepatic redox balance. Analysis of amino acids and their derivatives revealed high levels of anserine, taurine, and carnosine, supporting their antioxidant and anti-inflammatory roles. Collectively, our results highlight the hepatoprotective effects of CMH against APAP-induced liver injury, supporting its potential as a functional food ingredient for liver health and mitigating APAP-induced acute liver injury.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101863"},"PeriodicalIF":4.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of pear tree biochar on the growth and oxidative stress of Yunnanopilia longistaminea - A forest vegetable planted in copper-contaminated soil","authors":"Gang Deng ,&nbsp;Chigui Xiao ,&nbsp;Shirui Tan ,&nbsp;Jiangshan Xie ,&nbsp;Zhiyuan Liu ,&nbsp;Likai Xu ,&nbsp;Liu Yang ,&nbsp;Muzammal Rehman ,&nbsp;Shah Fahad ,&nbsp;Shikang Shen","doi":"10.1016/j.jafr.2025.101864","DOIUrl":"10.1016/j.jafr.2025.101864","url":null,"abstract":"<div><div>Biochar, an environmentally sustainable material, has been extensively used for the remediation of degraded soils. <em>Yunnanopilia longistaminea</em> is an edible plant and a valuable germplasm resource with significant economic importance in China. This study explored the potential of adding pear tree twigs biochar (PTB) to copper (Cu) contaminated soils, examining its effects on the growth and oxidative stress of <em>Yunnanopilia longistaminea</em>, as well as on soil health. A glasshouse study was conducted to find the efficacy of PTB at 0 %, 1 %, 3 %, and 5 % w/w on <em>Y. longistaminea</em> seedlings growth, oxidative stress, Cu uptake and post-harvest soil characteristics. The addition of PTB to the soil significantly improved seedling growth, as evidenced by increased fresh biomass, plant height, and SPAD readings, while also reducing the available Cu content in the soil. Chlorophyll in leaves was significantly high at 3 % w/w PTB rate, compared with the control. Moreover, 3 % PTB addition resulted in a significant reduction in oxidative stress and increase in soluble sugars and proteins. Furthermore, a prominent decline was detected in <em>Y. longistaminea</em> root, leaf, and stem Cu contents. Thus, PTB showed positive results for Cu immobilization in contaminated soil by improving soil electrical conductivity and reducing Cu uptake in <em>Y. longistaminea</em> seedlings. A negative correlation was observed between plant biomass and available Cu in soil. Our results highlight the significance of <em>Y. longistaminea</em> plantation using PTB in Cu contaminated soils.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101864"},"PeriodicalIF":4.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}