Virology & Immunology Journal最新文献

{"title":"Laboratory Based Preliminary Study on Anti-SARS-CoV-2 Antibody Response among State Sector Healthcare Workers in a Single District in Sri Lanka","authors":"J. Abeynayake","doi":"10.23880/vij-16000300","DOIUrl":"https://doi.org/10.23880/vij-16000300","url":null,"abstract":"Background: Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is responsible for the current COVID-19 pandemic. Healthcare workers are one of the risk groups due to their strategic role in patient management. Presence of antiSARS-CoV-2antibodies in serum following seroconversion could be determined through antibody ELISA assay. Objectives: This study was to analyze the laboratory identified viral markers of Anti SARS-CoV-2 antibody in blood samples of health care workers to demonstrate the seroconversion to SARS-CoV-2 virus following exposures, natural infection or vaccination and to describe the sociodemographic, and clinical parameters among them. Study design: This laboratory based retrospective study was conducted at the National Virus Reference Laboratory (NVRL), at the Medical Research Institute (MRI). The study retrospectively analyzed 235 blood samples received to the NVRL, for testing of anti-SARS-CoV-2 antibody among health care workers in a one month period. All samples requested for anti-SARSCoV-2 antibody were tested with SARS-CoV-2 spike protein specific IgG antibody, and with SARS-CoV-2 Total Ab ELISA. The socio-demographic data were gathered through the request forms were also analyzed. Results: Total 235blood samples were tested for anti-SARS-CoV-2 antibody and 234(99.6%) were confirmed to have spike protein specific IgG antibody while 2% were confirmed to have Nucleo-capsid protein targeting antibodies of the positives, 100% were vaccinated with two doses of vaccine and 1% had positive PCR. Conclusion: The current study encounters a significantly high prevalence of vaccine induces SARS-CoV-2 antibody carriers among the healthcare workers in the study community.","PeriodicalId":334586,"journal":{"name":"Virology & Immunology Journal","volume":"183-185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130675919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen Infection Associated Male Infertility and Role of Assisted Reproductive Techniques: A Review","authors":"Das P","doi":"10.23880/vij-16000297","DOIUrl":"https://doi.org/10.23880/vij-16000297","url":null,"abstract":"Recent research has revealed that pathogens in the semen and in the genital tract play a significant role in male infertility. The infection of viruses like Human Immunodeficiency Virus (HIV), Hepatitis B virus (HBV), Hepatitis C virus (HCV), Human Papilloma Virus (HPV), Herpes-Simplex Virus (HSV), and Human Cytomegalovirus (HCMV) may have a significant impact on male infertility. These infections may lead to temporary or permanent infertility, hormonal impairment, testicular malfunction, or abnormal spermatogenesis. This review is looking into the mechanism of viral infections and their effects on male fertility. The current review also focuses on defining the role of ARTs (Assisted Reproductive Techniques) in the occurrence and management of viral load of the male reproductive system","PeriodicalId":334586,"journal":{"name":"Virology & Immunology Journal","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132655398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLR 1,3 Receptor Gene Polymorphism in Egyptian Patients With Behcets Disease","authors":"A. A","doi":"10.23880/vij-16000298","DOIUrl":"https://doi.org/10.23880/vij-16000298","url":null,"abstract":"Behçet’s disease (BD) is a multisystem inflammatory disease characterized by recurrent orogenital ulcerations, ocular inflammations, and skin lesions. The ethology of the disease is currently unknown but evidences suggested that there is a strong genetic component mediating the chronicity of the disorder. The disease is characterized by infiltration of lymphocytes and neutrophils into the affected organs. APCs (Antigen Presenting Cells): It express receptor called (TLR) Toll like receptor which are one of PRR (pattern recognition receptor) and essential components of the innate immune system and they are a class of proteins play a key role in the innate immune system ,recognize structurally conserved molecules derived from microbes. This study aimed at investigating the possible associations between two SNPs (Single Nucleotide Polymorphisms) at TLR1 gene and TLR3 gene and BD in 87 Egyptian patients with BD and 87 healthy controls. Methodology: Blood samples were collected and DNA extraction done. Genotyping of TLR1 gene (1805 T/G), and TLR3 gene (1377 C/T) were performed using (PCR-RFLP) and we found In TLR1 gene (1805 T/G) • Patients with BD had significantly lower frequency of TT genotype and significantly higher frequency of TG , GG genotypes than healthy control • Patients with arthritis had significantly lower frequency of TT & TG, patients with activity had significantly lower frequency of GG genotype. • Patients with activity had significantly lower frequency of T allele, it might be protective allele and patients with arthritis had significantly lower frequency of G allele, it might be protective allele. In TLR3 gene (1377 C/T) • Patients with BD had significantly lower frequency of CC genotype and higher frequency of CT genotype than healthy control. • Patients with vascular involvement had significantly lower frequency of TT genotype. • BD patients with vascular involvement had significantly lower frequency of C allele than patients without involvement it might be protective allele. In conclusion, this preliminary study indicates that there are some genotypes in TLR1 gene (1805 T/G) ,and TLR3 gene (1377 C/T) over-represented , other less presented in BD indicating that they may play a role in BD susceptibility in Egyptian patients.","PeriodicalId":334586,"journal":{"name":"Virology & Immunology Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122079575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Anti-Human Papillomavirus IgG Antibody among Students of a Private Tertiary Institution in Ado-Ekiti, Nigeria","authors":"A. Ry","doi":"10.23880/vij-16000291","DOIUrl":"https://doi.org/10.23880/vij-16000291","url":null,"abstract":"Background: Human Papillomavirus (HPV) is an oncogenic sexually transmitted virus. It is the leading cause of cervical cancer, penile, vulva, vaginal and anal high-grade dysplasia. Infections are usually asymptomatic only to manifest after several years, implying that most adults with symptoms must have been infected at their young ages. The risky sexual behavior of subjects at ages 16-24 makes then vulnerable. Objectives: This study evaluates the prevalence of anti-human Papillomavirus IgG antibody among students of a tertiary institution in Ado-Ekiti, Nigeria. Methods: One hundred and fifty (150) blood samples were collected from consenting students (100 females and 50 males) age 16-24 in Ado-Ekiti. A structured questionnaire was administered concurrently. Sera were assayed using the anti-HPV-IgG antibody ELISA Kit (Melsin Medical Co. Limited). Data was analyzed using Epi-Info 7.2 Version Software and Excel. Ethical approval was obtained for the study. Results: Sixty eight (45%) of the total subjects were seropositive for anti-HPV-IgG antibody. Twenty six (52%) males and 42 (41%) females were seropositive. There was no significant difference (p>0.05) in seropositivity across the ages and among the gender. Fifty nine (60%) of sexually active subjects were seropositive for anti-HPV-IgG antibody (p˂0.05). Thirty five (97%) non-condom users were seropositive (p<0.05) while fifty four (75%) of subjects who have had unprotected sex at one point in their lives were seropositive (p< 0.05). Only three of the subjects had been vaccinated. Conclusion: The high rate of seropositivity to anti-HPV-IgG antibody in the population is a cardinal pointer to high rate of exposure to HPV and a likely exposure to the high-risk serotype.","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127174279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Contributing to COVID-19 Vaccine Hesitancy among Taxi Drivers in Juba City-South Sudan","authors":"Jibi Mc","doi":"10.23880/vij-16000295","DOIUrl":"https://doi.org/10.23880/vij-16000295","url":null,"abstract":"Introduction: Over the past few decades, vaccine hesitancy emerged as a major public health problem leading to outbreaks of communicable infections. While the development of a vaccine against COVID-19 was eagerly awaited by most of the communities at the beginning of the pandemic, skeptical voices are now becoming louder, and rising evidence shows mixed patterns with a worldwide rather decreasing trend of acceptance. The reasons for vaccine refusal are complex and differ according to geographical and cultural context. Objective: To find out factors contributing to COVID-19 vaccine hesitancy among taxi drivers in Juba City-South Sudan. Methodology: This research was a descriptive cross-sectional study designs which used quantitative research method. The study was conducted between February-May 2022. It targeted all the taxi drivers offering taxi service in formal/designated taxi park. To ensure better representation, all the designated/formal taxi parks in the three (3) payams of Juba City (Juba, Kator and Munuki) were purposely selected for the study and the numbers of the respondents were assigned equally to the three (3) taxi park. Total sample size of 384 was used and about 128 taxi drivers were interviewed in each taxi park. The daily registration lists of the taxi in the taxi parks were used as a sampling frame. Drivers were selected at a random from the sample frame using simple random sampling technique until the required number was met. A Self-administered close ended questionnaire was used to collect the data and responses from the questionnaires were manually validated and entered in excel spread sheet version 2019. It was analyzed and presented inform of tables, graphs, and pie-charts. Results: About 116 (30%) of the drivers were above the age of 35 years. Majority of the participants 376 (98%) heard about COVID-19 vaccine. Of those who heard about the COVID-19 vaccine, 201 (28%) stated that they heard through social media. When asked on their readiness to receive the COVID-19 vaccine, majority of the respondent 256 (67%) specified that they would not accept to be vaccinated if COVID-19 vaccine was offered to them. Amongst those who refused to be vaccinated, majority of the respondent 180 (26%) reported that they were concern on the adverse effects of the vaccine. Conclusion: Vaccine hesitancy is a major threat to curving down the COVID-19 pandemic. This research findings showed that majority of the respondent heard about COVID-19 vaccine using social media. Furthermore, this research established that majority of the taxi drivers would not accept to be vaccinated when the COVID-19 vaccine was offered to them due to fears of adverse effects of the vaccine; others were concern of acquiring COVID-19 infection from the vaccine itself","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132971419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Prognostic Factors of Severity in Covid-19 Patients in Lahore, Pakistan","authors":"J. K","doi":"10.23880/vij-16000296","DOIUrl":"https://doi.org/10.23880/vij-16000296","url":null,"abstract":"Background: The coronavirus disease outbreak of 2019 (COVID-19) has developed into a global emergency. COVID-19's severity is strongly correlated with its mortality rate. We aimed to examine the demographic, medical, laboratory, and imaging characteristics of 120 patients affected by COVID-19. Methods: 120 COVID-19 infected- patients who tested positive from the throat swab specimen on quantitative reverse transcriptase-polymerase chain reaction (q RT-PCR) were included. Demographic, clinical, radiographic, and laboratory data were obtained and analyzed. Results: Male gender, urban residence, current smoker, exposure history, sore-throat, dyspnoea, cough, headache, diarrhea, coronary heart disease, chronic obstructive lung disease, and cancer, decreased level of hemoglobin and albumin and increased level of lymphocyte count, leukocyte count, LDH level, D-dimer, serum ferritin, total bilirubin, urea level (p-value < 0.05) were contributing factors towards disease severity. In the binary logistic regression model LDH (OR 1.002, [CI, 1.001-1.003]; p = 0.001), and D-dimer (OR 1.377, [CI, 1.161-1.633]; p=0.001) were independent predictors of disease severity. Conclusion: Appropriate evaluation of prognostic factors and provision of the appropriate interventions in high-risk patients can decrease the mortality associated with COVID-19","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126417234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) by A Third Dose of Chadox1 Ncov-19 (Astrazeneca) After BNT162b2 (Pfizer–BioNTech)","authors":"Costa Rc","doi":"10.23880/vij-16000290","DOIUrl":"https://doi.org/10.23880/vij-16000290","url":null,"abstract":"An atypical case of VITT was described resulting from a vaccination schedule where the third booster with ChAdOx1 nCoV-19 (AstraZeneca) was administered. The patient received a complete vaccination schedule with two doses of Pfizer–BioNTech (BNT162b2) without any complications before the third dose. However, the patient has developed an infrequent yet extreme prothrombotic; hypercoagulable state caused by platelet-activating anti-platelet factor 4 (PF4) antibodies. This phenomenon is typically triggered by the proximate administration of an adenoviral vector vaccine against COVID-19. The patient’s symptoms began ten days after taking the third dose of the ChAdOx1 nCoV-19 vaccine (AstraZeneca). His main complaints when hospitalized were severe headaches and right abdominal pain. The blood tests and MRI scan imaging findings were very characteristic of VITT, and a rare cerebral venous sinus thrombosis was found. Also, a markedly elevated D-dimer and strong positive PF4-dependent enzyme- immunoassay test results were documented. Due to discerning clinical suspicion, this patient was rapidly treated with immunoglobulin infusion for two days and oral steroids for three days. Subsequently, he was anticoagulated with the new oral anticoagulant edoxaban after platelet numbers recovery. In a few days, platelets normalized, and D-dimer levels decreased, while anti- PF4-dependent enzyme-immunoassay test results showed a slow decline. He was discharged taking oral edoxaban without any squeal.","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130884539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global and Local Upsurge in Monkey pox Epidemic: Current Trends, Challenges and Future Prospects","authors":"Enitan Ss","doi":"10.23880/vij-16000292","DOIUrl":"https://doi.org/10.23880/vij-16000292","url":null,"abstract":"Amidst global relaxation of the Coronavirus Disease 2019 (COVID-19) intervention measures and celebration of the unprecedented grounds conquered in the fight against the pandemic, the monkeypox virus (MPXV) has emerged and remerged in many countries taking over major headlines around the globe at the present. Since 1 January and as of 22 June 2022, 50 nations and territories in five WHO Regions (Africa, America, Eastern Mediterranean, Europe, and Western Pacific) have reported 3413 laboratory-confirmed cases; along with one fatality. The Nigeria Centre for Disease Control (NCDC) received notice of a possible monkeypox epidemic in Bayelsa State on September 22, 2017. Thereafter, from Bayelsa to Rivers, Ekiti, Akwa Ibom, Lagos, Ogun and Cross River, the scourge of Monkeypox has continue to escalate five (5) years after the disease was first reported in Nigeria. An aggregate of 716 suspected cases have been recorded from 25 states nationwide between September 2017 and June 26, 2022. There have been 288 (40.2 percent) confirmed cases from 25 states out of the 716 suspected cases. So far, nine (9) deaths in total (CFR=3.1%) have been reported in 6 states between September 2017 and June 2022. At the moment, the following challenges are preventing the country from containing the virus effectively: 1) Low patient use of healthcare facilities because of stigma, cost, and low fatality rate; 2) Underreporting of cases due to inadequate access to healthcare facilities; 3) Low sample collection rate in reporting states; 4) Poor infectious disease laboratories support in remote areas; 5) Corruption and lack of political will by current administration to fight the virus; 6) Weak monkeypox monitoring, possibly related to a limited health workforce and the COVID 19 pandemic response; 7) A lack of international cooperation, unity, and coordination in the pursuit of establishing global health equity; and 8) Lack of access to authorized and safe treatment and vaccine among other things. Despite these challenges, a monkeypox-free Nigeria is possible. To this end, a safe and effective immunization of the public, as with smallpox, polio and yellow fever, is the key to the future. Until, a safe and effective vaccine becomes readily available, control and prevention lies largely on non-pharmaceutical intervention measures.","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"110 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113961773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resurgence of Monkeypox Virus Disease in Humans: A Review","authors":"R. A","doi":"10.23880/vij-16000293","DOIUrl":"https://doi.org/10.23880/vij-16000293","url":null,"abstract":"A once neglected zoonotic monkeypox virus endemic to West and Central Africa generated significant international interest, due to its emergence causing an outbreak in 2022 involving multiple countries in non-endemic regions. A total of 780 confirmed cases were reported worldwide (across 27 non-endemic countries) as of June 2, 2022, since 13 May 2022. Thus, human monkeypox is an emerging viral zoonotic disease recognized as the most important Orthopoxvirus infection in the smallpox post-eradication era. The waning herd immunity associated with discontinuation of smallpox vaccination may be responsible for resurgence of monkeypox. It is usually a self-limited disease, and the clinical presentation resembles that of smallpox, resulting in a rash similar to smallpox although clinically less severe. Monkeypox is transmitted to humans through close contact with an infected person or animal, or with material contaminated with the virus. Monkeypox virus is transmitted from one person to another by close contact with lesions, body fluids, respiratory droplets, and contaminated materials such as bedding, towels, and clothing. Antiviral drug developed for the treatment of smallpox has also been licensed for the treatment of monkeypox. The knowledge and scientific information on monkeypox virus disease is still unknown or relatively limited to many clinicians, and received little attention. In this review, we provide an overview of monkeypox virus infection to highlight its importance and further research","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124145564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of HBV DNA among HBsAg Negative Patients","authors":"Adeniyi AA.","doi":"10.23880/vij-16000294","DOIUrl":"https://doi.org/10.23880/vij-16000294","url":null,"abstract":"The persistence of Hepatitis B viral DNA (HBV DNA) in the blood or liver without the presence of detectable HBsAg is called Occult Hepatitis B infection (OBI)). This has become a threat when considering prevention of HBV transmission in our community. The purpose of this study was to determine prevalence as well as the HBV genotype(s) associated with OBI in Ibadan, Nigeria. HBsAg negative, using ELISA techniques, 219 blood samples were included for the study. Nested Polymerase Chain Reaction was used for the detection of HBV DNA in the samples. Data collected was processed using descriptive statistics and Chi square at p = 0.05. The number of male and female participants were 102 (46.6%) and 117 (53.4%) (P = 0.204) respectively. The participants have age range from 1 to 70 years with mean age of 32.1 years (SD =12.7) for females and 33.9years (SD = 12.4) for males. The clinical status of participants ranged from asymptomatic to those presented with acute hepatitis disease. Out of the 219 samples tested, one was positive for HBV DNA giving a prevalence of 0.5%. The positive sample was from a 30 years old female. We were unable to determine the genotype of the isolate due to poor sequence data. There is evidence of circulation of Occult Hepatitis B virus in our community as shown with prevalence of 0.5% OBI in this study. There is need for screening of blood units using Nucleic Acid Testing (NAT) technique to reduce the transmission of the virus through transfusion of infected blood and blood products. There is also need to increase awareness of HBV vaccination among the general population to forestall the danger OBI may pose.","PeriodicalId":334586,"journal":{"name":"Virology &amp; Immunology Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121472944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}