Ecotoxicology and Environmental Safety最新文献

{"title":"Transgenerational effects and mechanisms of Cry1Fa toxin on the non-target Myzus persicae","authors":"Liang Jin ,&nbsp;Binwu Zhang ,&nbsp;Peng Wang ,&nbsp;Yingqian Lei ,&nbsp;Yibin Pan ,&nbsp;Junyu Luo ,&nbsp;Jinjie Cui ,&nbsp;Yi Lin","doi":"10.1016/j.ecoenv.2025.119071","DOIUrl":"10.1016/j.ecoenv.2025.119071","url":null,"abstract":"<div><div>Although extensive research has focused on the effects of Cry toxins produced by <em>Bacillus thuringiensis</em> on target insects, fewer studies have explored their impacts and underlying mechanisms on non-target aphids. Herein, we investigated the effects and mechanisms of Cry1Fa toxin on the non-target peach aphid, <em>Myzus persicae</em>. Toxicity assessments confirmed that <em>M. persicae</em> was insensitive to Cry1Fa toxin. Subsequent transcriptome sequencing and identification of Cry1Fa toxin-binding proteins, along with life table analysis of three successive generations, revealed the transgenerational effects of Cry1Fa toxin on <em>M. persicae</em>. Treatment with Cry1Fa toxin did not significantly alter the life table parameters of the parent generation (F0), which was probably attributed to the significant up-regulation of autophagy-animal, ribosome, lysosome, and oxidative phosphorylation pathways. In the first filial generation (F1), the intrinsic rate of increase (<em>r</em>), finite rate of increase (<em>λ</em>), age-specific survival rate (<em>lx</em>), and age-specific life expectancy (<em>ex</em>) were significantly higher compared with the control group, suggesting a hormetic effect and the growth of aphid population in the F1 generation. The significant up-regulation of ribosome and oxidative phosphorylation pathways might account for these changes. However, the life table parameters of the second filial generation (F2) were not significantly affected, indicating a trade-off in resource allocation across generations. Our results provide valuable insights for the use and assessment of Cry toxins in pest control strategies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119071"},"PeriodicalIF":6.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145108085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the associations between prenatal PCB exposures and gene expression: Observations from a study of newborn Slovak infants","authors":"Amara Saleem ,&nbsp;Andrew Volz ,&nbsp;Tanmoy Mondal ,&nbsp;Christopher A. Loffredo ,&nbsp;Tomas Trnovec ,&nbsp;Lubica Palkovicova Murinova ,&nbsp;Kamil Conka ,&nbsp;Beata Drobna ,&nbsp;Somiranjan Ghosh","doi":"10.1016/j.ecoenv.2025.119059","DOIUrl":"10.1016/j.ecoenv.2025.119059","url":null,"abstract":"<div><div>Polychlorinated biphenyls (PCBs) are persistent organic pollutants known to have deleterious effects on child and adult development; however, less is known about the relationship between mother-newborn exposure levels. The objective of this study is to understand prenatal PCB exposure and its association with gene expression, using data from a cohort of exposed mothers and infants in eastern Slovakia. For 91 mothers and infants participating in this study, serum PCB concentrations were determined using gas chromatography and high-resolution mass spectrometry. Affymetrix microarray was performed utilizing Human Genome U133 Plus 2.0 gene chip. Statistical analysis compared the genetic expression levels of high versus low exposure mother-infant pairs (&lt; 368.57 ng/g lipid vs. &gt; 368.57 ng/g lipid). Statistical analysis results showed that mother's blood and cord blood PCB concentrations were highly correlated. Results showed that higher levels of PCB concentrations were associated with differential expressions of multiple genes. Global gene analysis identified significant dysregulation of genes XIST, EXOC6B, EIF1AY, and RPS4Y1. Gender-based gene analysis identified significant dysregulation of genes RUFY1, S100A8, ELOVL7, FXYD3, DEFB124, and DAB2. Further such investigations should be implemented to confirm these observations and more fully define the legacy of environmental PCB contamination.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119059"},"PeriodicalIF":6.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145108601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From exposure to pathogenesis: A critical review of SCCPs-induced toxicity and potential molecular mechanisms","authors":"Liyong Kou ,&nbsp;Hong Qi ,&nbsp;Yi Jiang ,&nbsp;Qizhan Liu ,&nbsp;Jing Sun","doi":"10.1016/j.ecoenv.2025.119078","DOIUrl":"10.1016/j.ecoenv.2025.119078","url":null,"abstract":"<div><div>Short-chained chlorinated paraffins (SCCPs) are a class of polychlorinated n-alkanes containing 10–13 carbon atoms, which are widely used in several industrial sectors due to their excellent thermal and chemical stability. However, their persistent residues, semi-volatility, and ubiquity in environmental media have raised global concerns. SCCPs are recognized as important environmental pollutants and are listed under the Stockholm Convention on Persistent Organic Pollutants due to their persistence, bioaccumulation, ability to be transported over long distances, and biotoxicity. Despite international restrictions, some developing countries continue to produce and use SCCPs. SCCPs have recently been found to be widely present in human blood, hair, and even in serum samples and umbilical cords of pregnant women. Exposure to SCCPs can lead to metabolic disorder and organ damage, including liver and kidney toxicity, and an increased risk of cancer. Epidemiologic studies have shown a positive correlation between serum levels of SCCPs and biomarkers of certain diseases, suggesting that SCCPs are potentially hazardous to health. Given the global public health implications of chronic exposure to SCCPs, a comprehensive health risk assessment is essential for effective control of health hazards. This review discusses the exposure routes, human accumulation, and mechanisms of toxicity of SCCPs, with a focus on neurologic, thyroid, hepatic, renal, and reproductive toxicity. In particular, the effects of SCCPs on metabolic disorders and signaling pathway perturbations are summarized, as well as the risk of exposure to SCCPs in human samples. Finally, we also look forward to the future direction of SCCPs toxicology research. It is recommended to focus on human exposomics to identify exposure biomarkers, integrate computational toxicology with organoid technologies, and provide scientific basis for SCCPs risk early warning and global management of persistent organic pollutants.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119078"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ozone exposure, retinal microvasculature alterations, and the mediating effect of aging in diabetic population: A retrospective cohort study","authors":"Senlin Lin ,&nbsp;Xia Meng ,&nbsp;Yi Xu ,&nbsp;Lina Lu ,&nbsp;Juzhao Zhang ,&nbsp;Saiguang Ling ,&nbsp;Minna Cheng ,&nbsp;Yuheng Wang ,&nbsp;Qinping Yang ,&nbsp;Haidong Kan ,&nbsp;Yan Shi ,&nbsp;Cong Liu ,&nbsp;Yingyan Ma ,&nbsp;Haidong Zou","doi":"10.1016/j.ecoenv.2025.119082","DOIUrl":"10.1016/j.ecoenv.2025.119082","url":null,"abstract":"<div><div>Accumulating epidemiological evidence links ozone exposure with cerebrocardiovascular morbidity, yet its microvascular impact remains poorly characterized. Retinal microvasculature alterations have emerged as sensitive biomarkers of systemic endothelial dysfunction and predictors of cerebrocardiovascular events. Our study comprised 55,463 individuals diagnosed with type 2 diabetes mellitus (T2DM), recruited across 249 community health centers spanning all 17 administrative districts of Shanghai in 2017. Exposure assessment utilized satellite-derived ozone concentration estimates at 1*1 km spatial resolution, aggregating five-year (2013–2017) ozone exposure metrics. Retinal vascular phenotyping included central retinal artery equivalent (CRAE), central retinal vein equivalent (CRVE), arteriole-to-venule ratio (AVR), vascular tortuosity (VT), and fractal dimension (D_f). The retinal age gap (RAG) is adopted to reflect accelerated aging, defined as the difference between deep learning-predicted retinal age and chronological age. Multivariable regression analyses using double machine learning showed that for every 10 μg/m³ increase in ozone, the decrease in the CRAE, CRVE, VT (×10⁴) and D_f (×10³) were 4.03 (95 %CI: 3.04, 5.01)μm, 8.35 (95 %CI: 6.69, 10.00)μm, 0.38 (95 %CI: 0.28, 0.48) and 13.7 (95 %CI: 11.20, 16.21), respectively, and the increase in RAG was 2.29 (95 %CI: 2.04, 2.54) years. Mediation analysis revealed an association between ozone exposure and reductions in CRAE (indirect effect, standardized coefficient=-0.08, 95 %CI −0.09∼-0.07), CRVE (-0.07, −0.08∼-0.06), and D_f (-0.11, −0.12∼-0.09) through the pathway of accelerated aging. Long-term ozone exposure correlates with accelerated retinal aging and microvascular remodeling (vessel narrowing, reduced tortuosity, fractal simplification), which provides novel biological evidence to elucidate the causal relationship between ozone exposure and cerebrocardiovascular risk.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119082"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental cadmium exposure promotes lung cancer via DHX34: A molecular toxicology perspective","authors":"Yongbin Lu ,&nbsp;Weize Kong ,&nbsp;Kaiwen Wang ,&nbsp;Zicong Shao ,&nbsp;Xu Hui ,&nbsp;Xuping Song ,&nbsp;Siyu Zhang ,&nbsp;Li Ma ,&nbsp;Zhiyuan Cheng ,&nbsp;Fei Su ,&nbsp;Tao Zhang ,&nbsp;Kehu Yang","doi":"10.1016/j.ecoenv.2025.119087","DOIUrl":"10.1016/j.ecoenv.2025.119087","url":null,"abstract":"<div><div>Although cadmium (Cd) exposure has been implicated in lung cancer development, systematic investigations into its association with cancer mortality, particularly lung cancer mortality remain limited, and the molecular mechanisms driving Cd-induced tumor progression are not fully understood. In this study, we first conducted a meta-analysis of existing cohort studies to quantitatively assess the association between Cd exposure and cancer- and lung cancer-specific mortality. We then employed an integrative approach combining bioinformatics analyses, LASSO regression, and Mendelian randomization to identify and validate DHX34 as a key gene implicated in Cd-related lung carcinogenesis. These findings were further supported by molecular docking, molecular dynamics simulations, in vitro functional assays, and in vivo tumor models. Our meta-analysis showed that long-term Cd exposure significantly increased cancer mortality risk, especially in males (<em>RR = 1.49, 95 % CI: 1.13–1.96</em>) and in lung cancer (<em>RR = 1.86, 95 % CI: 1.36–2.54</em>). Integration of GSE165549 and TCGA data identified 36 Cd-related genes enriched in tumor associated pathways including cell cycle and DNA replication. LASSO regression and Mendelian randomization suggested a causal role of DHX34 in lung cancer. Molecular docking demonstrated a strong binding affinity between Cd^2 + and DHX34 (binding free energy = –5.34 kcal/mol), and molecular dynamics simulations confirmed the stability of this complex. Functional assays further showed that CdCl₂ exposure upregulated DHX34, thereby promoting lung cancer cell proliferation and tumor growth both in vitro and in vivo. Together, these findings provide multi-level evidence that DHX34 mediates Cd-induced lung cancer progression, highlighting the carcinogenic potential of environmental heavy metal exposure and offering new insights into molecular targets for early prevention, risk stratification, and therapeutic intervention.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119087"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute cardiovascular biomarker responses to ambient PM2.5 and its constituents: A panel study on healthy retirees","authors":"Zhen An ,&nbsp;Lingling Shen ,&nbsp;Yange Zhang ,&nbsp;Ying Wang ,&nbsp;Ke Lu ,&nbsp;Hui Wu ,&nbsp;Jie Song ,&nbsp;Laibao Zhuo ,&nbsp;Juan Li ,&nbsp;Weidong Wu","doi":"10.1016/j.ecoenv.2025.119083","DOIUrl":"10.1016/j.ecoenv.2025.119083","url":null,"abstract":"<div><div>Ambient fine particulate matter (PM_2.5) remains a environmental risk factor for cardiovascular disease (CVD). However, the specific roles of PM_2.5 and its chemical components in cardiovascular events and the underlying mechanisms have not been fully understood. This study aimed to unravel the associations of short-term exposure to PM_2.5 and its constituents with adverse cardiovascular outcomes. A panel study of healthy retirees was conducted and a linear mixed-effects model used to examine the relationships between PM_2.5, its 18 chemical components, and key cardiovascular biomarkers. Primary outcomes included blood pressure (BP), heart rate (HR), heart rate variability (HRV), interleukin (IL)-6, IL-8, high-sensitivity C-reactive protein (hs-CRP), 8-hydroxy-2’-deoxyguanosine (8-OHdG), tissue-type plasminogen activator (t-PA), platelet monocyte aggregates (PMAs), endothelin-1 (ET-1), creatine kinase (CK), and lactate dehydrogenase (LDH). In addition, stratification by gender and <em>glutathione S-transferase theta 1</em> (<em>GSTT1</em>) polymorphism was performed. Results showed that short-term PM_2.5 exposure was significantly linked to increased systolic BP (SBP), mean arterial pressure (MAP), HR, IL-6, and ET-1, along with decreases in t-PA and HRV. Metallic components (Mn, Ni, As, Se, Rb, Sr, Mo, Sb, and Cs) and ionic components (F⁻, NO₃⁻, PO₄³⁻, and SO₄²⁻) were particularly associated with elevated BP and reduced HRV. Moreover, gender and <em>GSTT1</em> polymorphism modified susceptibility to these cardiovascular effects. Overall, these findings suggest that short-term PM_2.5 exposure induces significant cardiovascular and biochemical changes in retirees, including elevated BP, HR, systemic inflammation, endothelial dysfunction, coagulation disturbances, and reduced HRV. PM_2.5 constituents and individual factors, such as gender and <em>GSTT1</em> polymorphism, contribute to these effects.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119083"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal variation of the hydrogeochemical characteristics, formation mechanism, and pollution assessment of shallow groundwater in Karamay, China","authors":"Huiming Yu ,&nbsp;Li Chen ,&nbsp;Hongguang He ,&nbsp;Wei Wang ,&nbsp;Qingqing Guan ,&nbsp;Xianmin Ke","doi":"10.1016/j.ecoenv.2025.119080","DOIUrl":"10.1016/j.ecoenv.2025.119080","url":null,"abstract":"<div><div>Groundwater is an important water resource in the Karamay region, which is located in an arid area in northwestern China. A comprehensive understanding of groundwater chemistry and quality is essential for the sustainable development and effective management of water resources. However, the hydrogeochemical characteristics, formation mechanism, and pollution characteristics of groundwater in this region remain unclear. Hence, this study analyzed 274 groundwater samples, collected in the wet and dry seasons, using multivariate statistical analysis. Basic statistical results showed that groundwater was weakly alkaline to alkaline with high TDS, and the average cation and anion concentrations followed K⁺ &lt;Mg²⁺&lt;Ca²⁺&lt;Na⁺ and CO₃^2–&lt;HCO₃^–&lt;SO₄^2–&lt;Cl^–, respectively. High values of SO₄^2–, Cl^–, Na⁺, Ca²⁺, Mg²⁺, and K⁺ were mainly concentrated in Baijiantan District and the northern part of Karamay District. Groundwater was dominated by the NaCl type, which was mainly controlled by evaporation and rock weathering, and the control pattern remained stable between the wet and dry seasons. The major components of groundwater primarily originated from the dissolution of minerals, including unsaturated halite and gypsum, as well as saturated dolomite and calcite. Cation exchange played a significant role in groundwater evolution. The ratios of polluted areas to the total area were 63.19–70.72 % and 62.55–68.30 % in the wet and dry seasons, respectively, indicating very poor groundwater quality. These findings can provide insights into the evolution of the groundwater environment in arid regions, and guide water resources management and protection in ecologically fragile areas.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119080"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental reproductive toxicity of common pesticides: Mechanisms and implications throughout the zebrafish life cycle","authors":"Lin Yang ,&nbsp;Lin Li ,&nbsp;Zhiqiang Kong ,&nbsp;Yifan Zhang ,&nbsp;Frédéric Francis ,&nbsp;Bei Fan ,&nbsp;Minmin Li","doi":"10.1016/j.ecoenv.2025.119029","DOIUrl":"10.1016/j.ecoenv.2025.119029","url":null,"abstract":"<div><div>Reproductive toxicity is a significant factor contributing to the decline in population numbers and is increasingly being reported in association with pesticide exposure in ecological risk assessments. Pesticides can leach or spill into aquatic environments, where they remain suspended or settle, making aquatic organisms vulnerable to potential adverse effects. Although considerable progress has been made in studying the environmental impacts on reproductive health, there remains a need for comprehensive reviews detailing the complex relationships between reproductive toxicity and the structural characteristics of pesticides. The aim of this review is to summarize the diverse effects of various types of pesticides on the development and functionality of reproductive organs, gametes, embryos, and reproductive behaviors. This review discusses the potential mechanisms underlying these effects, such as disruptions to the hypothalamus-pituitary-gonad-liver (HPGL) axis, interference with steroid synthesis, DNA damage and methylation, inhibition of aromatase activity, as well as other detrimental impacts like oxidative stress and apoptosis. The interplay between these factors and the aforementioned mechanisms is intricate and can trigger a cascade of effects that ultimately compromise fertility and offspring viability. In summary, understanding how pesticides affect reproductive health in zebrafish will help us better protect aquatic ecosystems and, ultimately, human health. In addition to elucidating these mechanisms, understanding their interactions is crucial for the comprehensive assessment of reproductive toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119029"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological risk assessment using a multi-scale approach in the estuarine copepod Eurytemora affinis applied to an ecdysone agonist","authors":"Caroline Arcanjo ,&nbsp;Yannick Colin ,&nbsp;Nathalie Giusti-Petrucciani ,&nbsp;Aurélie Duflot ,&nbsp;Jean-Luc Ravanat ,&nbsp;Gauthier Trémolet ,&nbsp;Romain Coulaud ,&nbsp;Anne-Laure Vivant ,&nbsp;Benoit Xuereb ,&nbsp;Thierry Berthe ,&nbsp;Joëlle Forget-Leray ,&nbsp;Céline Boulangé-Lecomte","doi":"10.1016/j.ecoenv.2025.119081","DOIUrl":"10.1016/j.ecoenv.2025.119081","url":null,"abstract":"<div><div>Previous transcriptomic analyses have demonstrated that exposure to tebufenozide (TEB) induces significant alterations in gene expression in the crustacean <em>Eurytemora affinis</em> (calanoid copepod; <em>E. affinis</em>), supporting its suitability as a model organism for investigating the ecotoxicological effects of ecdysone agonists in estuarine environments. Based on these findings, we hypothesized that TEB exposure may disrupt biological processes across multiple levels of organization in <em>E. affinis</em>. We conducted an integrated assessment of upstream (epigenetics) and downstream (locomotion, larval development) endpoints through multiple assays. Additionally, we examine the indirect effects of TEB by evaluating the changes in the copepod microbiota. We showed that TEB had no impact on global DNA methylation (HPLC-MS/MS) in both males and females. In contrast, significant effects were observed on the swimming behaviour of males, with copepods exposed to TEB being more affected by the light/dark transition and showing hyperactivity after 4 days. The larval development was also shown to be accelerated by TEB after 4 days. Finally, significant changes in microbiota were highlighted, including a decrease in both alpha and beta diversity indices, along with an increase in the abundance of the Gammaproteobacteria class. The effects observed in <em>E. affinis</em> show that TEB could impact fitness and increase vulnerability of non-target organisms. The strategy of prioritising the responses to be investigated on the basis of gene expression has proven effective. The study reinforces the relevance of using <em>E. affinis</em> for environmental risk assessment in estuaries, as it can be used to assess multiple endpoints at different biological scales.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119081"},"PeriodicalIF":6.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mediating role of gut microbiota in the influence of PM2.5 exposure on gestational diabetes mellitus","authors":"Yan Long ,&nbsp;Weitao Ye ,&nbsp;Xueqing Cen ,&nbsp;Hongmei Jin ,&nbsp;Shanshan Mei ,&nbsp;Jingyi Ye ,&nbsp;Xueqin Zhao ,&nbsp;Xiaoyan Zhang ,&nbsp;Xiaoli Gao ,&nbsp;Chunyan Zhu","doi":"10.1016/j.ecoenv.2025.119070","DOIUrl":"10.1016/j.ecoenv.2025.119070","url":null,"abstract":"<div><h3>Objective</h3><div>Our study aimed to explore the mediating effects of the gut microbiota on the associations of PM_2.5 exposure with gestational diabetes mellitus (GDM).</div></div><div><h3>Methods</h3><div>Participants (n = 1268) were recruited in Guangzhou, China. The participants’ information and fecal samples were collected, the air pollutants exposures during pregnancy was assessed using measurements from the nearest monitoring station, and the relative abundance of fecal microbiota was characterized by 16S rRNA sequencing. Linear discriminant, mediation, and regression analyses were conducted to estimate the associations among PM_2.5 exposure, gut microbiota and GDM.</div></div><div><h3>Results</h3><div>Elevated PM_2.5 exposure was significantly correlated with the alterations in the gut microbiota, and with the increased risks of GDM and impaired glucose homeostasis. The mediation analyses indicated that alterations in the gut microbiota partially mediated the effects of PM_2.5 exposure on GDM and impaired glucose homeostasis. The total effects of PM_2.5 on GDM were mediated by g-Phascolarctobacterium (1.83 %), s-Phascolarctobacterium_unclassified (1.83 %), and s-Bacteroides_uniformis (0.65 %). The corresponding mediating effects for increased fasting glucose level were 3.48 %, 8.55 %, 1.76 %, 5.14 % and 0.02 % by g-Bacteroides, g-Lachnospiraceae_Ruminococcus, g-Streptococcus, g-Dorea and s-Bacteroides_uniformis, respectively.</div></div><div><h3>Conclusions</h3><div>Elevated PM_2.5 exposure from 3 months before pregnancy to the 12th week of gestation may induce GDM via alteration of the gut microbiota.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119070"},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}