Ecotoxicology and Environmental Safety最新文献

{"title":"Association between indoor air pollution exposure and dyslipidemia among Chinese adults: The role of leisure-time physical activity","authors":"Yajie Li ,&nbsp;Jianbo Li ,&nbsp;Wangmu Cidan ,&nbsp;Li Yin ,&nbsp;Qucuo Nima ,&nbsp;Xianzhi Li","doi":"10.1016/j.ecoenv.2025.119170","DOIUrl":"10.1016/j.ecoenv.2025.119170","url":null,"abstract":"<div><h3>Background</h3><div>Dyslipidemia, a major factor for cardiovascular diseases, has been linked to air pollution exposure. However, evidence on the association between indoor air pollution (IAP) from solid fuel use and dyslipidemia remains limited, as does the potential mitigating role of leisure-time physical activity (LTPA).</div></div><div><h3>Methods</h3><div>This cross-sectional study analyzed data from 69,680 adults in the China Multi-Ethnic Cohort (CMEC). IAP exposure was assessed based on self-reported solid fuel use for cooking and heating. Dyslipidemia was defined using blood lipid measurements. LTPA was quantified in metabolic equivalent task-hours per week (MET-h/week). Multivariable logistic regression and interaction analyses were conducted to evaluate associations and synergistic effects.</div></div><div><h3>Results</h3><div>Each 10 MET-h/day increase in LTPA was associated with a 9 % reduction in the prevalence of high non-HDL-C (OR = 0.91, 95 % CI: 0.84–0.98) and a 13 % reduction in hypertriglyceridemia (OR = 0.87, 95 % CI: 0.82–0.92). Solid fuel use for cooking was significantly associated with an increased prevalence of hypertriglyceridemia (OR = 1.14, 95 % CI: 1.09–1.20) and hyperbetalipoproteinemia (OR = 1.20, 95 % CI: 1.13–1.28), while, solid fuel use for heating was linked to higher prevalence of hypertriglyceridemia (OR = 1.34, 95 % CI: 1.27–1.42) and hypoalphalipoproteinemia (OR = 1.13, 95 % CI: 1.05–1.21). LTPA attenuated these associations, with significant multiplicative and additive-scale interactions observed (e.g., for heating fuel use and LTPA on dyslipidemia: P-interaction &lt; 0.05, RERI = 0.18, 95 % CI: 0.08–0.27). Participants using solid fuels and engaging in low LTPA had the highest prevalence of dyslipidemia. Moreover, subgroup analyses confirmed stronger adverse effects of IAP in low-LTPA individuals.</div></div><div><h3>Conclusions</h3><div>Household solid fuel use is associated with dyslipidemia, particularly hypertriglyceridemia and hyperbetalipoproteinemia. LTPA may mitigate these associations, highlighting the dual importance of clean energy adoption and physical activity (PA) promotion in reducing dyslipidemia burden. Public health strategies should target high- populations, such as rural communities with limited access to clean fuels.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"305 ","pages":"Article 119170"},"PeriodicalIF":6.1,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic oxygen vacancy reconstruction in MnO2 polymorphs builds electron reservoirs to drive ionic charge redistribution for stepwise UO22 + reduction","authors":"Shuang Zhang ,&nbsp;Zixiong Wu ,&nbsp;Wenting Zhu ,&nbsp;Tao Bo ,&nbsp;Yingcai Wang ,&nbsp;Hao Jiang ,&nbsp;Xiaoyan Li ,&nbsp;Yibao Liu ,&nbsp;Yuhui Liu","doi":"10.1016/j.ecoenv.2025.119167","DOIUrl":"10.1016/j.ecoenv.2025.119167","url":null,"abstract":"<div><div>Effective uranium (UO₂²⁺) recovery from aqueous waste is vital for resource sustainability and environmental safety. Although oxygen vacancies enhance the redox activity of UO₂²⁺, their long–term stability and ability to regenerate remain unclear. Herein, we engineered α–, β–, γ–, and δ–MnO₂ through controlled NaH₂PO₂ reduction to create tunable oxygen vacancies. During the modulation of oxygen vacancies, partial phase transitions were induced, with α–MnO₂ and γ–MnO₂ maximizing structural adaptability and performance. Defect–rich α– and γ–MnO₂ exhibit significantly accelerated UO₂²⁺ reduction kinetics, retaining over 90.0 % of their initial activity after multiple redox cycles. Correspondingly, α–MnO₂–OVs and γ–MnO₂–OVs achieve maximum UO₂²⁺ adsorption capacities of 581.0 and 492.0 mg g⁻¹, respectively. In situ XRD reveals that oxygen vacancies act as reversible active sites for UO₂²⁺ adsorption and reduction in real rare–earth leachates, regenerating through lattice–oxygen recombination in the U<img>O bond during redox cycling, thereby maintaining high catalytic activity. DFT shows that oxygen vacancies create unsaturated Mn sites that form Mn–O–U bridges, directing electrons from Mn 3d into U 5 f orbitals. The near–zero U density of states at the Fermi level confirms this Mn–O vacancy network acts as a localized electron reservoir, lowering the energy barrier for sequential UO₂²⁺ reduction. This work highlights cyclic oxygen vacancy engineering as an effective approach for efficient actinide separation and sustainable environmental remediation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119167"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of disruption of the gut–brain axis by environmental endocrine disruptors","authors":"Hao Yao,&nbsp;Jie Yu,&nbsp;Xiao Yang,&nbsp;Jie Xu","doi":"10.1016/j.ecoenv.2025.119124","DOIUrl":"10.1016/j.ecoenv.2025.119124","url":null,"abstract":"<div><div>Environmental endocrine disruptors (EEDs) are exogenous chemicals that impair physiological health by disrupting endocrine function. The gut–brain axis represents a complex bidirectional communication network integrating the gut microbiome, immune system, neural signaling, and endocrine pathways to maintain systemic homeostasis. Within this interconnected system, gut microbiota influence mood regulation, immune activity modulates neural processes, and neural signaling governs circadian and sleep cycles. This review explores the multi-system impacts of EEDs across four key physiological domains: (1) gut microbial ecology, (2) immune function, (3) neuroendocrine regulation, and (4) developmental processes. Evidence indicates that EED exposure disrupts intestinal microbial composition, leading to dysbiosis marked by the depletion of beneficial taxa and the expansion of pathogenic species. Concurrently, EEDs impair gut-associated immune cell populations (T cells, B cells, and macrophages), undermining mucosal immunity and increasing susceptibility to inflammatory bowel disease, autoimmune conditions, and gastrointestinal malignancies. At the endocrine level, EEDs interfere with the hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes, contributing to hormonal imbalances and impaired reproductive development. Neurochemically, they disrupt the synthesis, release, and degradation of key neurotransmitters, including norepinephrine, dopamine, and serotonin, while exerting direct neurotoxic effects such as cerebrovascular abnormalities and delayed cerebellar myelination. In summary, this review delineates the mechanistic pathways through which EEDs perturb gut–brain axis homeostasis. These insights provide a scientific basis for designing targeted therapeutic interventions and shaping evidence-based public health policies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119124"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dicofol induces developmental cardiotoxicity in zebrafish embryos through oxidative stress mechanisms","authors":"Wenfeng He ,&nbsp;Si Dong ,&nbsp;Fasheng Liu ,&nbsp;Yunyun Du ,&nbsp;Shanshan Zhu ,&nbsp;Yulian Li ,&nbsp;Yongliang Zheng ,&nbsp;Ting Ding ,&nbsp;Jinmei Shao ,&nbsp;Weifang Gao ,&nbsp;Qian Ouyang ,&nbsp;Huiling Liu ,&nbsp;Xiaoping Wang ,&nbsp;Junquan Zeng","doi":"10.1016/j.ecoenv.2025.119166","DOIUrl":"10.1016/j.ecoenv.2025.119166","url":null,"abstract":"<div><div>Dicofol (DCF), a widely used organochlorine acaricide, has raised global concerns due to its persistence, bioaccumulation, and toxicity. Although banned in many developed countries, DCF is still used in some regions, posing ecological and health risks. Here, we investigated the developmental cardiotoxicity of DCF in zebrafish embryos and its underlying mechanisms. DCF exposure caused ventricular reduction, atrial enlargement, pericardial edema, intracardiac blood congestion, reduced heart rate, and an increased sinus venosus–bulbus arteriosus (SV–BA) distance. These defects were associated with altered expression of cardiac development genes, indicating disrupted transcriptional regulation. Transcriptomic analysis revealed that DCF disrupts mitochondrial oxidative phosphorylation, leading to reduced ATP production and excessive reactive oxygen species (ROS) generation. Impaired antioxidant defenses further destabilized cardiac developmental gene networks and promoted cardiomyocyte injury and apoptosis. Biochemical assays confirmed elevated ROS levels, lipid peroxidation, and reduced antioxidant enzyme activity, while acridine orange staining demonstrated increased cardiomyocyte apoptosis. Notably, co-treatment with astaxanthin (ASTA) effectively mitigated DCF-induced cardiac defects, restored gene expression, and reduced oxidative damage. These findings identify oxidative stress as a key mediator of DCF-induced cardiotoxicity and demonstrate the potential of natural antioxidants in alleviating pesticide toxicity. This study offers mechanistic insights into the developmental hazards of legacy organochlorine pesticides and emphasizes the need for stricter environmental regulation and safer alternatives.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119166"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational exposure to TDCIPP disrupts embryonic development via LEPR-mediated IL6/JAK2/STAT3 signaling pathway in mice","authors":"Yongbo Zhao ,&nbsp;Hanyu Rao ,&nbsp;Ping Qiao ,&nbsp;Zhiyi Pan ,&nbsp;Yan Zhao ,&nbsp;Liping Jin","doi":"10.1016/j.ecoenv.2025.119168","DOIUrl":"10.1016/j.ecoenv.2025.119168","url":null,"abstract":"<div><div>Organophosphate esters (OPEs) are widely used flame retardants that have become ubiquitous in the environment. As typical environmental endocrine disruptors (EEDs), their adverse effects on human reproduction and development have raised global concern. In this study, we assessed the embryotoxicity of tris (1,3-dichloro-2-propyl) phosphate (TDCIPP, the most frequently detected OPEs in environment and human) and illustrated the molecular mechanisms in mouse embryos in vivo and <em>in vitro</em>. We found that exposure to 1 mg/kg⋅bw/day TDCIPP from gestational day (GD) 1 to GD14 significantly induced embryonic absorption and deformity in pregnant mice. Similar embryonic absorption and deformity were observed in in vitro fertilization (IVF) mice model after TDCIPP-treated embryos were transferred. Mechanistically, we found that exposure to TDCIPP downregulated the expression of leptin receptor (LEPR) and inhibited the downstream signaling pathway of interleukin 6 (IL6) /janus kinase 2 (JAK2) /signal transducer and activator of the transcription 3 (STAT3). In embryo culture system, the supplementation of 100 ng/mL IL6 or 50 ng/mL IL10 markedly alleviated TDCIPP-induced embryonic absorption and deformity. Collectively, our results suggest that the inhibition of LEPR-mediated IL6/JAK2/STAT3 signaling pathway might play a role in TDCIPP-induced embryotoxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119168"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-related differences in photosynthesis, energy metabolism, and antioxidant regulation of Neoporphyra haitanensis to ultraviolet-B stress","authors":"Song Xue ,&nbsp;Jun Chen ,&nbsp;Jiayi Xin ,&nbsp;Shuo Liang ,&nbsp;Hongzhen Wang ,&nbsp;Xinqi Li ,&nbsp;Lei Liu ,&nbsp;Liuqing Tang ,&nbsp;Yu Zang ,&nbsp;Xuexi Tang","doi":"10.1016/j.ecoenv.2025.119117","DOIUrl":"10.1016/j.ecoenv.2025.119117","url":null,"abstract":"<div><div>Increased ultraviolet-B (UVB) radiation due to human activities poses a considerable threat to all exposed organisms. While sex-related differences in stress responses have been observed in many plant species, little is known about whether such differences exist in macroalgae. In this study, we investigated the physiological, transcriptomic, and metabolomic responses of male and female <em>Neoporphyra haitanensis</em> under UVB stress and subsequent recovery. The physiological results indicated that UVB stress strongly inhibited the photosynthetic pigment content and activity in females compared to those in males. Consequently, females exhibited higher levels of reactive oxygen species (ROS) and malondialdehyde (MDA) production under UVB stress and recovery. In contrast, males exhibited stronger antioxidant enzyme activity to alleviate the oxidative stress under UVB stress. Transcriptomic analysis revealed that UVB caused a greater downregulation of genes related to pigment synthesis, photosystems, and carbon fixation in females, including genes encoding magnesium protoporphyrin IX methyltransferase (ChlM), phycoerythrin (PE), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, males possessed a stronger photosynthetic recovery capacity, as a greater number of photosynthesis-related genes were significantly upregulated in males after recovery. Under UVB stress, the expression of genes encoding key enzymes involved in glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle was higher in males than in females, indicating a stronger energy metabolism capacity in males. Metabolomic data emphasized the protective role of nonenzymatic antioxidants, particularly flavonoids, with females accumulating more methylated flavonoids and flavonoid glycosides after recovery than males. Overall, these findings highlight sex-related differences in photosynthesis, energy metabolism, and antioxidant regulation in <em>N. haitanensis</em> under UVB stress and recovery. These results contribute to our understanding of how sex differences in macroalgae influence their population survival under increased UVB radiation and provide insights into their potential adaptive responses to ongoing climate change.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119117"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanisms underlying effects of oxygenated polycyclic aromatic hydrocarbons exposure on inflammatory bowel disease","authors":"Yufang Hou ,&nbsp;Yuexia Che ,&nbsp;Tiegang Li ,&nbsp;Zheng Yan ,&nbsp;Wenyi Zhao ,&nbsp;Silin Lv ,&nbsp;Fang Zhang ,&nbsp;Mingxuan Zhou ,&nbsp;Yixin Zhou ,&nbsp;Zengni Zhu ,&nbsp;Xinyi Ren ,&nbsp;Siying Huang ,&nbsp;Min Yang","doi":"10.1016/j.ecoenv.2025.119153","DOIUrl":"10.1016/j.ecoenv.2025.119153","url":null,"abstract":"<div><div>Oxygenated polycyclic aromatic hydrocarbons (OPAHs), emerging contaminants in the environment, demonstrate heightened toxicity versus their parent compounds, creating substantial health hazards. Inflammatory bowel disease (IBD) encompasses chronic conditions marked by severe inflammation in the digestive tract. Environmental factors contribute to the development of both types of IBD. However, the toxicological mechanisms underlying OPAHs-induced IBD remain poorly understood. This investigation methodically examined the potential connection between OPAHs and IBD, identifying key genes involved in OPAHs-induced IBD. Using a network toxicology approach, 324 overlapping genes were identified between disease targets and five representative OPAHs effectors. These genes were further refined to 15 potential targets through analysis with STRING and Cytoscape. GO and KEGG pathway enrichment analyses indicated these targets primarily participate in oxidative stress responses, cell death, chemical stress reactions, inflammatory processes, and immune pathways. Subsequent analysis with machine learning algorithms highlighted three core targets: AKT1, ALB, and APP. Immune landscape analysis and single-cell RNA sequencing demonstrated strong associations between these hub genes and immune cell infiltration and immune responses. Molecular docking revealed high binding affinities between OPAHs and these hub proteins. <em>In vitro</em> experiments confirmed that OPAHs significantly elevated reactive oxygen species levels and induced intestinal epithelial barrier dysfunction. This study elucidates the molecular mechanisms through which OPAHs influence IBD development, offering valuable insights into the health risks posed by environmental pollutants and identifying potential targets for therapeutic development.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119153"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the carcinogenic role of benzo[a]pyrene in glioblastoma: Insights from network toxicology, single-cell transcriptomics, and Mendelian randomization","authors":"Liye Yi ,&nbsp;Wencai Wang ,&nbsp;Zhonghua Sun ,&nbsp;Yinuo Chen ,&nbsp;Zijie Xiong ,&nbsp;Luyao Ma ,&nbsp;Wei Ye ,&nbsp;Xianfeng Li","doi":"10.1016/j.ecoenv.2025.119155","DOIUrl":"10.1016/j.ecoenv.2025.119155","url":null,"abstract":"<div><h3>Background</h3><div>Benzo[<em>a</em>]pyrene (BaP) is a known environmental carcinogen linked to multiple tumors, but its role in glioblastoma (GBM) remains poorly understood. This study aimed to explore BaP’s tumorigenic mechanisms in GBM through an integrated approach combining network toxicology, single-cell transcriptomics, Mendelian randomization, and bibliometric analysis.</div></div><div><h3>Methods</h3><div>BaP target genes were predicted using ChEMBL, SEA, and PharmMapper, and GBM-related genes were retrieved from GeneCards, OMIM, and TTD. Overlapping genes were used to construct a protein–protein interaction network in Cytoscape. Molecular docking and molecular dynamics simulations were performed to assess BaP–target interactions. Single-cell RNA-seq data (GSE131928) were analyzed to profile gene expression in GBM subpopulations. Mendelian randomization assessed causal relationships between core genes and GBM risk, and findings were validated in vitro. Bibliometric analysis tracked research trends on these genes.</div></div><div><h3>Results</h3><div>31 overlapping genes were identified. TP53 was highly expressed in MES-like and AC-like malignant cells as well as CD8⁺ Tex cells. MR revealed a significant inverse association between TP53 expression and GBM risk (OR = 0.13, 95 % CI: 0.04–0.47, p = 0.002). Docking and simulation showed strong BaP–TP53 binding, confirmed by in vitro experiments. Bibliometrics indicated that TP53 research in GBM has shifted from basic mechanisms to clinical translation.</div></div><div><h3>Conclusion</h3><div>BaP may drive GBM by targeting TP53, offering insights for GBM prevention and therapy.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119155"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of microplastics and excessive nitrogen pollution on oat growth and soil nitrogen cycling","authors":"Hengkang Xu,&nbsp;Chao Chen,&nbsp;Zhuo Pang,&nbsp;Guofang Zhang,&nbsp;Weiei Zhang,&nbsp;Haiming Kan","doi":"10.1016/j.ecoenv.2025.119119","DOIUrl":"10.1016/j.ecoenv.2025.119119","url":null,"abstract":"<div><div>Both excessive nitrogen (N) and microplastic (MPs) pollution pose global change challenges to ecosystems and human health. The effects of MPs coexisting with excessive N on plant growth and N cycling are still largely unknown. This study employed a pot experiment to assess how polypropylene (PP) MPs influence oat (<em>Avena sativa</em> L.) growth and soil N cycling under conditions of excessive N fertilization. In the treatments of excessive N treatment, 2 % PP MPs significantly increased underground biomass (54.5 %). ¹⁵N stable isotope results showed that 2 % PP MPs treatment significantly reduced the percentage of N from fertilizer in oat by 10.4 %. Compared to without N treatment, excessive N treatment reduces the Shannon index of the rhizosphere soil bacterial community. Only at the 0.5 % PP MPs level, excessive N treatment significantly reduced the diversity of bacterial communities in the bulk soil compared to the without N treatment. The Shannon index of fungal community was not affected by N treatment and MPs. Microbial biomass nitrogen (MBN) and pH were significant related to sensitive species in microbial communities. The coexistence of excess N and 2 % PP MPs significantly reduced the abundance of genes (<em>norB</em>, <em>nosZ</em> and <em>nirB</em>) related to denitrification process. Structural equation modeling showed that MPs could promote underground biomass, reduce soil pH, inorganic N content, and reduce N uptake efficiency, thus resisting growth damage caused by high N. In the future, the effects of different concentrations and types of MPs and different nitrogen forms on soil and crops should be further investigated.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119119"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-tiered workflow for constructing a quantitative adverse outcome pathway network for gamma radiation-induced reproductive inhibition in Lemna minor","authors":"Li Xie ,&nbsp;Knut Erik Tollefsen","doi":"10.1016/j.ecoenv.2025.119129","DOIUrl":"10.1016/j.ecoenv.2025.119129","url":null,"abstract":"<div><div>While numerous qualitative adverse outcome pathways (AOPs) have been developed to describe the progressive toxicity of ionizing radiation, quantitative AOPs (qAOPs) to detail how effects quantitatively propagate from the initial molecular interaction to an adverse outcome remains limited. This study addresses this gap by proposing a multi-tiered workflow for developing a qAOP network (qAOPN) to characterise the detailed relationship between deposition of ionizing energy and decreased population growth rate in aquatic macrophytes, based on published quantitative dose-response (DR) data for gamma (Co-60) radiation-induced reproductive inhibition in the aquatic plant <em>Lemna minor</em>. First, a preliminary AOPN was assembled for the effects of ionizing radiation on plants captured in established AOPs #386, #387, and #388 (<span><span>www.aopwiki.org</span><svg><path></path></svg></span>). Weight of evidence (WoE) assessment from the AI-informed tool AOP-helpFinder and a narrative review were then applied to validate the confidence of individual key events relationships (KERs). Further quantification of the KERs was performed using a combination of point of departure (PoD) analysis via benchmark dose (BMD) modelling, structure equation modelling (SEM) and multiple nonlinear regression modelling (MNLRM). This approach effectively identified the most sensitive events within the AOPN and determined which linear AOPs were most relevant for low-dose radiation exposure. The resulting multi-tiered analysis enhanced the understanding of the underlying toxicity pathways, identified data gaps, and demonstrate how quantitative qAOPNs can be used to predict adverse (apical) impacts. The study presents a generic workflow for constructing qAOPNs that can expand the utility of mechanistically-informative AOPs towards quantitative models applicable both to chemical and non-chemical stressors.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119129"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}