Ecotoxicology and Environmental Safety最新文献

{"title":"Early-life polystyrene nanoplastics exposure impairs pathogen avoidance behavior associated with intestine-derived insulin-like neuropeptide (ins-11) and serotonin signaling in Caenorhabditis elegans","authors":"Chan-Wei Yu,&nbsp;Pei-Ling Yen,&nbsp;Yu-Hsuan Kuo,&nbsp;Ting-An Lin,&nbsp;Vivian Hsiu-Chuan Liao","doi":"10.1016/j.ecoenv.2024.117347","DOIUrl":"10.1016/j.ecoenv.2024.117347","url":null,"abstract":"<div><div>Nanoplastics (NPs) contamination is an emerging global concern due to the widespread use of plastic products and their potentially negative health impact on ecosystems. Despite their ubiquity, the effects of early-life NPs exposure on host-pathogen interactions remain largely unknown. In this study, we show that early-life exposure to polystyrene NPs (PS-NPs, 100-nm) at predicted environmentally relevant concentrations (10 µg/L) significantly impairs food preference and reduces avoidance of the pathogenic bacterium <em>Bacillus thuringiensis</em> in <em>Caenorhabditis elegans</em>. Exposure to PS-NPs led to a decrease in avoidance from 40.3 % in controls to 30.6 % at 10 µg/L and further to 23.1 % and 17.4 % at 50 and 100 µg/L, respectively. Mechanistic insights reveal that PS-NPs downregulate intestine-derived insulin-like neuropeptide (<em>ins-11</em>) via the transcription factor HLH-30 and the p38 MAPK signaling pathways, both are essential for avoidance behavior. Notably, acute serotonin treatment restored the avoidance behavior, indicating a role of serotonin signaling in this process. Our study indicates that early-life exposure to PS-NPs (100-nm) adversely affects the avoidance behavior of <em>C. elegans</em>, making them more vulnerable to harmful pathogens, thereby affecting their health. These findings highlight significant ecological and health hazards by early-life PS-NPs exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117347"},"PeriodicalIF":6.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport","authors":"Olha Lakhneko ,&nbsp;Ivana Fialová ,&nbsp;Roderik Fiala ,&nbsp;Mária Kopáčová ,&nbsp;Andrej Kováč ,&nbsp;Maksym Danchenko","doi":"10.1016/j.ecoenv.2024.117334","DOIUrl":"10.1016/j.ecoenv.2024.117334","url":null,"abstract":"<div><div>Nickel is an essential micronutrient for plant growth and development. However, in excessive amounts caused by accidental pollution of soils, this heavy metal is toxic to plants. Although silicon is a non-essential nutrient, it accumulates in most monocots, particularly the vital crop maize (corn, <em>Zea mays</em>). In fact, this metalloid mineral can alleviate the toxicity of heavy metals, though the mechanism is not entirely clear yet. Herein, we measured proteome, gene expression, enzyme activities, and selected sugars to investigate such effect thoroughly. Deep proteomic analysis revealed a minor impact of 100 µM Ni, 2.5 mM Si, or their combination on roots in 12-day-old hydroponically grown maize seedlings upon 9 days of exposure. Nonetheless, we suggested plausible mechanisms of Si mitigation of excessive Ni: Chelation by metallothioneins and phytochelatins, detoxification by glycine betaine pathway, and restructuring of plasma membrane transporters. Higher activity of glutathione S-transferase confirmed its plausible involvement in reducing Ni toxicity in combined treatment. Accumulation of sucrose synthase and corresponding soluble sugars in Ni and combined treatment implied high energy requirements both during heavy metal stress and its mitigation. Expression analysis of genes coding a few differentially accumulated proteins failed to reveal concordant changes, indicating posttranscriptional regulation. Proposed mitigation mechanisms should be functionally validated in follow-up studies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117334"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hexavalent chromium induced metabolic reprogramming, carcinogenesis and tumor progression through PDK1 upregulation","authors":"Wen-Jing Liu ,&nbsp;Lin Wang ,&nbsp;Fan-Li Sun ,&nbsp;Feng-Mei Zhou ,&nbsp;Rui-Ke Zhang ,&nbsp;Jie Liu ,&nbsp;Min Zhao ,&nbsp;Li-Hong Wang ,&nbsp;Yan-Ru Qin ,&nbsp;Yan-Qiu Zhao ,&nbsp;Jian-Ge Qiu ,&nbsp;Bing-Hua Jiang","doi":"10.1016/j.ecoenv.2024.117341","DOIUrl":"10.1016/j.ecoenv.2024.117341","url":null,"abstract":"<div><div>Lung cancer is the leading factor of cancer-related death in the worldwide. Hexavalent chromium [Cr(VI)] is a potential carcinogen for inducing lung cancers. To understand new mechanism of Cr(VI)-induced tumorigenesis and cancer development, we identified that PDK1 expression levels were greatly increased in chromium-transformed cells (Cr-T) compared to the parental BEAS-2B (B2B) cells by proteomic profiling and Western blotting; PDK1 levels were also induced in lung cancer cell lines and in lung samples of mice exposed to Cr(VI). Cr(VI) increased Warburg effect, cell migration, proliferation and colony formation through PDK1 upregulation. To identify the mechanism of PDK1 induction, we performed miRNA-seq analysis of Cr-T and B2B cells, and found miR-493 levels was significantly suppressed by Cr(VI). PDK1 was induced by miR-493 suppression, and was a direct target of miR-493. Interestingly, we also found HIF-1α was directly targeting by miR-493 and was induced by miR-493 downregulation. HIF-1α expression levels were upregulated in lung samples of mice with Cr(VI)-exposure. PDK1 was induced by HIF-1α, showing miR-493 suppression can directly induce PDK1 as well as through HIF-1α induction. MiR-493 overexpression was sufficient to suppress tumor growth, PDK1 and HIF-1α expression <em>in vivo</em>. We also showed that levels of miR-493 suppression, HIF-1α and PDK1 elevations were strongly correlated with poor prognosis of lung cancer subjects. These results demonstrate both HIF-1α and PDK1 expression are induced by Cr(VI)-mediated miR-493 suppression, and MiR-493/HIF-1α/PDK1 axis is a new pathway in Cr(VI)-inducing carcinogenesis and tumor growth.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117341"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating tobacco smoke-induced craniofacial deformities: Biomarker and MAPK signaling dysregulation unraveled by cross-species multi-omics analysis","authors":"Yuxin Lin ,&nbsp;Hao Li ,&nbsp;Shukai Zheng ,&nbsp;Rui Han ,&nbsp;Kusheng Wu ,&nbsp;Shijie Tang ,&nbsp;Xiaoping Zhong ,&nbsp;Jiasheng Chen","doi":"10.1016/j.ecoenv.2024.117343","DOIUrl":"10.1016/j.ecoenv.2024.117343","url":null,"abstract":"<div><div>Tobacco smoke (TS), particularly secondhand and thirdhand smoke, poses a pervasive and intractable environmental hazard that promotes teratogenesis and the progression of craniofacial malformations, although the underlying mechanisms remain elusive. Using zebrafish larvae as a model, our research demonstrated a correlation between the increasing concentration of cigarette smoke extract (CSE) and the severity of craniofacial malformations, supported by Alcian blue staining and histological assessments. Through a combined mRNA-miRNA analysis and quantitative real-time PCR, we identified <em>miR-96–5p</em>, <em>miR-152</em>, <em>miR-125b-2–3p</em>, and <em>miR-181a-3–3p</em> as pivotal biomarkers in craniofacial cartilage development. Functional analyses revealed their association with the MAPK signaling pathway, oxidative stress (OS), and cell development, highlighting MAPK as a crucial mediator. Single-cell transcriptomics further disclosed aberrant MAPK activation in mesenchymal cells. Subsequent investigations in human embryonic palatal mesenchymal (HEPM) cells confirmed similar patterns of growth inhibition, apoptosis, and OS, and emphasized the cross-species consistency of these biomarkers and the over-activation of the MAPK signaling pathway. A comprehensive tri-omics analysis of HEPM cells identified pivotal genes, proteins, and metabolites within the MAPK pathway. This groundbreaking cross-species multi-omics study unveils novel biomarkers and MAPK pathway perturbations linked to TS-induced craniofacial developmental toxicity, promoting innovative clinical prediction, diagnosis, and interventional strategies to tackle TS-induced craniofacial malformations.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117343"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental contamination by veterinary medicinal products and their implications in the conservation of the endangered Pyrenean Capercaillie (Tetrao urogallus aquitanicus)","authors":"Olga Nicolás de Francisco ,&nbsp;Ana Carolina Ewbank ,&nbsp;Ana de la Torre ,&nbsp;Irene Sacristán ,&nbsp;Ivan Afonso Jordana ,&nbsp;Anna Planella ,&nbsp;Oriol Grau ,&nbsp;Diego Garcia Ferré ,&nbsp;Josep Maria Olmo-Vidal ,&nbsp;Antonio J. García-Fernández ,&nbsp;Isabel Navas ,&nbsp;Antoni Margalida ,&nbsp;Carlos Sacristán","doi":"10.1016/j.ecoenv.2024.117299","DOIUrl":"10.1016/j.ecoenv.2024.117299","url":null,"abstract":"<div><div>The endangered Pyrenean Capercaillie (<em>Tetrao urogallus aquitanicus</em>) inhabits perennial forests of the Pyrenees (Spain, France and Andorre). Feces of domestic animals (e.g., horses and cattle) are often found in this species’ habitat as evidence of land use overlapping, especially during spring and summer. As a result, pharmaceutical residues found in feces of these domestic ungulates may be absorbed by plants and insects that are part of the diet of Pyrenean Capercaillies (e.g., blueberries [<em>Vaccinium uliginosum, Vaccinium myrtillus</em>], red wood ants [<em>Formica rufa</em>]). Based on the absence of data regarding the exposure of Pyrenean Capercaillie to residues of veterinary medicinal products (VMP), we selected 71 compounds as indicators of anthropogenically-related environmental contamination, analyzed in 90 samples collected in several subalpine forests, northwestern Spain. Residues of several VMP were detected in feces (capercaillie [ciprofloxacin, enrofloxacin, tetracycline and florfenicol], horse [ciprofloxacin, enrofloxacin, tetracycline and ivermectin], and cattle [ciprofloxacin and enrofloxacin]), and in entomofauna (ciprofloxacin and ivermectin). No VMP residues were detected in blueberry plants. Herein, we present novel data about the presence of VMP residues in the Pyrenean Capercaillie’s environment, and identify potential VMP sources (i.e., livestock feces and entomofauna) and an exposure route (i.e., food chain) for Capercaillie chicks. Further studies are necessary to investigate the potential indirect or chronic effects of VMP residues in the species’ breeding success and adult fitness, which must be taken into account by managers and policy makers to improve management and conservation actions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117299"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophytes mitigate Microcystis aeruginosa-induced fish appetite suppression via intestinal metabolite regulation","authors":"Minmin Niu ,&nbsp;Keira Harshaw ,&nbsp;Qianqian Xiang ,&nbsp;Yuan Zhou ,&nbsp;Ping Xiang ,&nbsp;Zhihao Ju ,&nbsp;Wenyu Long ,&nbsp;Hugh J. MacIsaac ,&nbsp;Xuexiu Chang","doi":"10.1016/j.ecoenv.2024.117348","DOIUrl":"10.1016/j.ecoenv.2024.117348","url":null,"abstract":"<div><div>Cyanobacterial blooms and aquatic macrophytes can affect the health, physiology, and behavior of freshwater fish. Changes in food intake can be a key indicator of stress in teleost fish, while changes in metabolite abundance in the gut can indicate a shift in metabolic priorities, including response to environmental stressors. Here, we exposed stone moroko (<em>Pseudorasbora parva</em>) to the cyanobacterium <em>Microcystis aeruginosa</em> and/or the macrophyte <em>Ottelia acuminata</em> and analyzed changes in fish health, appetite regulation, and intestinal metabolome after 96-h exposures. We found that <em>O. acuminata</em> treatment didn’t change the tested indicators, while exposure to <em>M. aeruginosa</em> increased concentrations of appetite-inhibiting factors, such as CART and GLP-1, and decreased concentrations of stimulatory factors like orexin. Exploration of the metabolome following exposure revealed that the appetite-inhibiting influence of <em>M. aeruginosa</em> was positively correlated with key metabolites of lipid, amino acid, and cholesterol metabolism, especially those associated with bile acid synthesis and secretion. Further, the presence of <em>O. acuminata</em> decreased the adverse effects of <em>M. aeruginosa</em> among neuro-endocrine regulatory factors, which could be explained by altered regulation of intestinal amino acid metabolites. The deeper mechanism by which <em>O. acuminata</em> moderates the harmful effects of <em>M. aeruginosa</em> remains to be identified.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117348"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of 3D and 2D in vitro models of the permanent fish liver cell line RTL-W1: Metabolic capabilities and responses to xenobiotics","authors":"Vanessa Holgersson ,&nbsp;Shelby Joyce ,&nbsp;Marianne Brookman-Amissah,&nbsp;Tobias Lammel","doi":"10.1016/j.ecoenv.2024.117327","DOIUrl":"10.1016/j.ecoenv.2024.117327","url":null,"abstract":"<div><div>In vitro models based on permanent fish liver cell lines have proven to be versatile tools for examining chemical biotransformation and toxicity. However, their in vivo relevance remains uncertain due to their potentially de-differentiated phenotype. Here, we investigate whether a 3D cell culture environment can restore hepatocyte-like properties of the Rainbow trout liver cell line RTL-W1. Utilizing ultralow attachment (ULA) microwell plates, we achieved controlled sizing and extended culture (3 weeks) of spheroidal aggregate cultures (spheroids). RTL-W1 cells within the spheroids remained viable and metabolically active, as confirmed by the CellTiter-Glo 3D assay. Transmission electron microscopy revealed that spheroids exhibit tissue-like arrangements, such as interdigitations, cell-cell junctions, and endo- or exocytic activity at the cell-cell interface. They also displayed ultrastructural characteristics typical of metabolically active cells/hepatocytes, including abundant endoplasmic reticulum (ER), Golgi apparatus, and mitochondria. RT-qPCR analysis showed upregulation of genes involved in xenobiotic and endogenous (lipid) metabolism in 3D cultures over time. Notably, for several genes, especially cyp1a, expression levels were significantly higher in spheroids than in monolayers cultured for the same duration. This was corroborated at the enzyme level by increased Cyp1a-dependent catalytic activity (EROD). Interestingly, increased Cyp1a expression did not lead to heightened susceptibility to benzo[<em>a</em>]pyrene toxicity, which requires bioactivation. However, RTL-W1 3D and 2D cell cultures exhibited differential susceptibility to toxicity from other model chemicals, such as the surfactant SDS and the metal copper (Cu). These findings support the hypothesis that RTL-W1 cells can re-differentiate to a hepatocyte-like phenotype when cultured in a 3D configuration and may exhibit distinct biological responses upon exposure to xenobiotics. Overall, this study advances our understanding of the potential of cell line-derived 3D in vitro models for research and providing more physiologically relevant data for regulatory contexts.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117327"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental triggers and future risk of developing autoimmune diseases: Molecular mechanism and network toxicology analysis of bisphenol A","authors":"Yanggang Hong ,&nbsp;Deqi Wang ,&nbsp;Yinfang Lin ,&nbsp;Qianru Yang ,&nbsp;Yi Wang ,&nbsp;Yuanyuan Xie ,&nbsp;Wanyi Shu ,&nbsp;Sheng Gao ,&nbsp;Chunyan Hua","doi":"10.1016/j.ecoenv.2024.117352","DOIUrl":"10.1016/j.ecoenv.2024.117352","url":null,"abstract":"<div><div>Bisphenol A (BPA), a chemical compound in plastics and resins, widely exist in people's production and life which have great potential to damage human and animal health. It has been proved that BPA could affect human immune function and promote the occurrence and development of autoimmune diseases (ADs). However, the mechanism and pathophysiology remain unknown. Therefore, this study aims to advance network toxicology strategies to efficiently investigate the putative toxicity and underlying molecular mechanisms of environmental pollutants, focusing on ADs induced by BPA exposure. Leveraging databases including ChEMBL, STITCH, SwissTargetPrediction, GeneCards, and OMIM, we identified potential targets associated with BPA exposure and ADs, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), multiple sclerosis (MS), Hashimoto's thyroiditis (HT), inflammatory bowel disease (IBD), and type 1 diabetes (T1D). Subsequent refinement using STRING and Cytoscape software highlighted core targets respectively, and Metascape was utilized for enrichment analysis. Gene expression data from the GEO database revealed the upregulation or downregulation of these targets across these ADs. Molecular docking performed with Autodock confirmed robust binding between BPA and core targets, notably PPARG, CTNNB1, ESR1, EGFR, SRC, and CCND1. These findings suggest that BPA exposure may serve as an environmental trigger in the development of autoimmunity, underscoring potential environmental risk factors for the onset of autoimmune conditions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117352"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endosulfan promotes cell growth, migration and invasion via CCL5/CCR5 axis in MCF-7 cells","authors":"Zeming Liu ,&nbsp;Xiaolin Ding ,&nbsp;Boxiang Zhang ,&nbsp;Yue Pang ,&nbsp;Yuhui Wang ,&nbsp;Dan Xu ,&nbsp;Hailong Wang","doi":"10.1016/j.ecoenv.2024.117344","DOIUrl":"10.1016/j.ecoenv.2024.117344","url":null,"abstract":"<div><div>Endosulfan, recognized as an endocrine disruptor, has emerged as an important risk factor for human breast cancer. The chemokine ligand 5 (CCL5) and its receptor CCR5 constitute a biological axis, that is implicated in tumorigenesis and cancer progression. However, the role of the CCL5/CCR5 axis in breast cancer when exposure to endosulfan remains unclear. The present study aimed to determine the significance of the CCL5/CCR5 axis in the carcinogenic effects of endosulfan in human breast cancer MCF-7 cells. The results showed that endosulfan significantly promoted cell proliferation, increased the rate of colony formation, and enhanced cell migration ability in a dose-dependent manner by activating the PI3K/AKT signaling pathway, which were rescued by the specific inhibitor (LY-294002) for PI3K/AKT signaling pathway. We utilized Cytoscape software to construct protein-protein interaction (PPI) network when exposure to endosulfan, and identified 47 highly connected genes in the network diagram centered on CCL5. Endosulfan significantly increased the secretion of CCL5 and the expression levels of CCL5/CCR5, which were reversed by CCR5 inhibitor (HY-13004). HY-13004 significantly counteracted the effects of endosulfan on colony formation, cell migration and the activation of PI3K/AKT signaling pathway. Endosulfan markedly altered the expression levels of epithelial-mesenchymal transition (EMT) biomarkers and enhanced transwell migration and invasion capabilities of MCF-7 cells, which were inhibited by HY-13004, similar to the effects observed with LY-294002. Collectively, our findings suggest that endosulfan activates the PI3K/AKT signaling pathway to promote cell growth, and induces EMT, thereby enhancing cell migration and invasion via the CCL5/CCR5 axis in MCF-7 cells.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117344"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The correlation between heavy metal chelation and transcriptional potential of GRAS genes in Broussonetia papyrifera","authors":"Dapei Li ,&nbsp;Chenhao Li ,&nbsp;Shen Yang ,&nbsp;Ying Lu ,&nbsp;Yan Tang ,&nbsp;Zhenggang Xu ,&nbsp;Shaobing Peng ,&nbsp;Guiyan Yang","doi":"10.1016/j.ecoenv.2024.117342","DOIUrl":"10.1016/j.ecoenv.2024.117342","url":null,"abstract":"<div><div>In order to understand the adaptation mechanism of <em>Broussonetia papyrifera</em> to heavy metal stress and then promote its remediation and utilization, in this study, a total of 24 GRAS transcription factors were identified from <em>B. papyrifera</em> transcriptomes. Their complete ORFs were 597–2250 bp in length with encoding proteins 22.40–84.13 kDa. The 24 <em>BpGRAS</em>s were distributed across nine chromosomes and two scaffolds. Their promoters contained numerous <em>cis</em>-acting elements involving in plant development, environmental stimuli, and hormonal regulation. These <em>BpGRAS</em> genes were predominantly transcribed in flowers and fruits. The most prominent genes were <em>BpSCL21b</em> and <em>BpDELLA1</em>, whose expression levels in flowers were 4.11- and 4.56-fold higher than the minimal one in leaves. All <em>BpGRAS</em>s were apparently induced by ABA and at least one treatment of Cd, Cu, Mn, and Zn. The expression of some <em>BpGRAS</em> genes (including <em>BpSCL1d</em>, <em>BpSCL7</em>, <em>BpSCL27</em>, <em>BpSCL34</em>, etc.) was significantly correlated with HM chelation and the non-protein thiols (NPT) accumulation, which was regarded as barriers to resist HM stress, under Cd, Cu, Mn, and Zn stress. Moreover, <em>BpSCL15</em> and <em>BpSCL21b</em> transgenic yeast displayed significantly enhanced growth and viability (1.23-–2.71-fold, 1.30-–1.96-fold of control OD₆₀₀, accordingly) and metal accumulation (1.81-–3.58-fold, 1.91-–3.17-fold of control, accordingly). These results suggested that <em>BpGRAS</em>s, especial <em>BpSCL15</em>, <em>BpSCL21b</em>, and <em>BpSCL34</em>, are essential for <em>B. papyrifera</em> response to HM stress depending on ABA signaling. It’s the first time to investigate the correlation of <em>BpGRAS</em>s’ expression with HM and NPT accumulation, which may benefit for revealing the HM adaptation mechanism of <em>B. papyrifera</em> and provide candidate genes for HM resistance breeding in woody plants.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117342"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}