Ecotoxicology and Environmental Safety最新文献

Efficient removal of cadmium (II) and arsenic (III) from water by nano-zero-valent iron modified biochar-zeolite composite

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-13 DOI: 10.1016/j.ecoenv.2025.118178

Mengyuan Wu , Lijuan Wu , Wen Zhang , Xianbao Zhong , Runfeng Guo , Ziying Cui , Yajun Yang , Jialong Lv

{"title":"Efficient removal of cadmium (II) and arsenic (III) from water by nano-zero-valent iron modified biochar-zeolite composite","authors":"Mengyuan Wu , Lijuan Wu , Wen Zhang , Xianbao Zhong , Runfeng Guo , Ziying Cui , Yajun Yang , Jialong Lv","doi":"10.1016/j.ecoenv.2025.118178","DOIUrl":"10.1016/j.ecoenv.2025.118178","url":null,"abstract":"<div><div>For the removal of Cd(II) and As(III) from water, this study synthesized a nano-zero-valent iron-loaded biochar-zeolite composite material (nZVI-BCZo) using a liquid-phase reduction method, with biochar, zeolite, and FeSO₄·7H₂O as precursors. The successful incorporation of nZVI onto the BCZo was verified through Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), and Fourier Transform Infrared Spectroscopy (FTIR) analyses, which revealed significant modifications in the surface oxygen-containing functional groups. Batch adsorption experiments were conducted to evaluate the adsorption characteristics and performance of nZVI-BCZo for Cd(II) and As(III). Under optimal conditions (pH 6.0, temperature of 310 K, and an adsorption time of 360 min), the maximum adsorption capacities for Cd(II) and As(III) were found to be 28.09 mg/g and 186.99 mg/g, respectively. The influence of pH on removal efficiency was more pronounced than that of temperature, with nZVI-BCZo exhibiting a higher affinity for As(III) compared to Cd(II). Kinetic analysis showed that the adsorption process is primarily controlled by chemical adsorption and follows a monolayer adsorption mechanism. Regeneration tests demonstrated that nZVI-BCZo retained good adsorption capacity after three cycles, with adsorption efficiencies of 67.78 % for Cd(II) and 53.04 % for As(III), indicating its potential for repeated use in water treatment applications. The economic evaluation revealed that nZVI-BCZo has a lower processing cost. Therefore, this study established nZVI-BCZo as an efficient, reusable, and cost-effective adsorbent for the treatment of heavy metal-laden water.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118178"},"PeriodicalIF":6.2,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorantraniliprole-induced oxidative stress, DNA damage, and apoptosis in Caenorhabditis elegans: Mechanistic insights and ecological risk implications

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-13 DOI: 10.1016/j.ecoenv.2025.118164

Fengyuan Hu , Mengzhen Yang , Shaohua Han , Guokun Hu , Xiaoxue Ji , Kang Qiao

{"title":"Chlorantraniliprole-induced oxidative stress, DNA damage, and apoptosis in Caenorhabditis elegans: Mechanistic insights and ecological risk implications","authors":"Fengyuan Hu , Mengzhen Yang , Shaohua Han , Guokun Hu , Xiaoxue Ji , Kang Qiao","doi":"10.1016/j.ecoenv.2025.118164","DOIUrl":"10.1016/j.ecoenv.2025.118164","url":null,"abstract":"<div><div>Chlorantraniliprole (CAP) is one of the most widely used insecticides in the world. CAP is strictly restricted in foodstuff with maximum residual limits (MRLs) from 0.01 to 40 mg/kg set by Chinese national food safety standard. However, a detailed evaluation on its possible acute toxicity and the underlying mechanisms remains inconclusive. In this study, effects of CAP at environmentally relevant concentrations on growth, locomotion, lifespan, reproduction, and antioxidative defense systems were evaluated using the model organism <em>Caenorhabditis elegans</em>. Exposure to CAP notably reduced nematode development, head thrash, and pharyngeal pumping frequency compared with the control. Moreover, CAP at 0.1, 1, and 10 μg/L decreased lifespan of nematodes by 23.73 %, 28.71 %, and 36.23 %, respectively. CAP at 1 and 10 μg/L enhanced the ROS level, reduced the activity of antioxidative enzyme, including CAT and SOD. CAP also regulated mRNA expression levels of <em>daf-16</em>, <em>skn-1</em>, <em>sod-3</em>, <em>gst-4</em>, <em>ced-3</em>, <em>ced-4</em>, <em>ced-9</em>, <em>egl-1</em>, <em>clk-2</em>, and <em>hus-1</em> in the nematodes, while no significant effect in the mutants was observed. Pearson correlation analysis revealed that significant correlation existed between tested parameters, indicating that CAP caused a series of negative effects in the nematodes. Meanwhile, molecular docking results revealed the potential of CAP to bind with oxidative stress, DNA damage and apoptosis proteins, providing molecular mechanisms for the observed detrimental effects. Therefore, our results suggested that acute exposure to CAP at environmental concentrations caused oxidative stress, DNA damage and apoptosis in the nematodes<em>.</em> Our results shed new light on risk assessment and management of CAP.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118164"},"PeriodicalIF":6.2,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of microcystin contamination in fish across the Lake Chaohu basin under the Yangtze River ten-year fishing ban: Ecological and human health implications

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-12 DOI: 10.1016/j.ecoenv.2025.118185

Shengpan Han , Wulai Xia , Jun He , Qianhui Wu , Wenli Xu , Jia Yu , Jun Chen , Ping Xie

{"title":"Spatiotemporal dynamics of microcystin contamination in fish across the Lake Chaohu basin under the Yangtze River ten-year fishing ban: Ecological and human health implications","authors":"Shengpan Han , Wulai Xia , Jun He , Qianhui Wu , Wenli Xu , Jia Yu , Jun Chen , Ping Xie","doi":"10.1016/j.ecoenv.2025.118185","DOIUrl":"10.1016/j.ecoenv.2025.118185","url":null,"abstract":"<div><div>Microcystins (MCs), produced by harmful cyanobacterial blooms, pose a threat to aquatic ecosystems and public health in the Yangtze River basin, including Lake Chaohu, which implemented a ten-year fishing ban in 2021 for ecological restoration. This study provides the first basin-wide assessment of MC contamination in fish muscle tissues from Lake Chaohu and its major tributaries during the ban's initial phase (2022–2023), with comparisons to pre-ban data. Using UHPLC-MS/MS, we quantified MC-LR and MC-RR in fish from four trophic levels (planktivorous, omnivorous, herbivorous, and carnivorous). Results revealed that MC-RR was the predominant variant, and planktivorous fish exhibited the highest MC levels, followed by omnivorous, herbivorous, and carnivorous species, reflecting dynamics of trophic transfer. Overall, fish MC concentrations were significantly lower during the ban compared to pre-ban levels, demonstrating positive initial effects. Seasonal analysis revealed higher MC levels in warmer seasons, but detectable MCs persisted during cold seasons, reflecting ongoing contamination. Spatially, while fish in the lake exhibited higher MC concentrations, contamination was also detected in riverine fish, highlighting the basin-wide spread. Despite a lower overall health risk from fish consumption relative to the pre-ban period, high consumption of planktivorous species, especially during warm seasons and in lake regions, may still pose potential health risks. Additionally, MCs likely pose ecological risks to fish populations. These findings provide a crucial baseline for evaluating the long-term effectiveness of the fishing ban and underscore the need for integrated watershed management to safeguard both ecological and public health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118185"},"PeriodicalIF":6.2,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The environmental occurrence, human exposure, and toxicity of novel bisphenol S derivatives: A review

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-12 DOI: 10.1016/j.ecoenv.2025.118182

Ning Wu , Yinling He , Zhendong Sun , Shengnan Zhang , Xiaoxi Yang , Qian S. Liu , Qunfang Zhou , Guibin Jiang

{"title":"The environmental occurrence, human exposure, and toxicity of novel bisphenol S derivatives: A review","authors":"Ning Wu , Yinling He , Zhendong Sun , Shengnan Zhang , Xiaoxi Yang , Qian S. Liu , Qunfang Zhou , Guibin Jiang","doi":"10.1016/j.ecoenv.2025.118182","DOIUrl":"10.1016/j.ecoenv.2025.118182","url":null,"abstract":"<div><div>Novel bisphenol S (BPS) derivatives are being increasingly utilized as substitutes to bisphenol A (BPA) and BPS in thermal receipts and other industrial or commercial products. In recent years, the environmental occurrence, human exposure, and toxicity of non-chlorinated and chlorinated BPS derivatives have been investigated in numerous studies. This review summarizes the state-of-art and new knowledge on these aspects and provides recommendations for future research directions. The environmental analysis showed that BPS derivatives have been widely detected in paper products, water, indoor dust, sediment, and municipal sewage sludge. Recent studies have also reported the presence of non-chlorinated BPS derivatives, such as benzenesulfonylbenzene (DDS) and 4-(4-propan-2-yloxyphenyl)sulfonylphenol (BPSIP), in human breast milk, urine, and the maternal−fetal−placental unit. Toxicological studies suggest that BPS derivatives may cause a series of toxic effects, including endocrine-disrupting effects, cytotoxicity, hepatotoxicity, developmental toxicity, and neurotoxicity, some of which have been shown to exhibit adverse effects similar to or even greater than those of BPS. Future studies should focus on elucidating environmental occurrences, half-lives, sources for human exposure, and potential transformation pathways of BPS derivatives, as well as their toxic effects and underlying mechanisms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118182"},"PeriodicalIF":6.2,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyfloral nutritional resources promote bumble bee colony development after exposure to a pesticide mixture

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118170

Denise Castle , Abdulrahim T. Alkassab , Silvio Erler , Gabriela Bischoff , Falk Gerdes , Andrey Yurkov , Michael Steinert , Ingolf Steffan-Dewenter , Jens Pistorius

{"title":"Polyfloral nutritional resources promote bumble bee colony development after exposure to a pesticide mixture","authors":"Denise Castle , Abdulrahim T. Alkassab , Silvio Erler , Gabriela Bischoff , Falk Gerdes , Andrey Yurkov , Michael Steinert , Ingolf Steffan-Dewenter , Jens Pistorius","doi":"10.1016/j.ecoenv.2025.118170","DOIUrl":"10.1016/j.ecoenv.2025.118170","url":null,"abstract":"<div><div>Bumble bees are important pollinators of crops in the field and greenhouses. They are naturally exposed to a combination of interacting stressors, e.g., loss of flowering resources and exposure to plant protection products. Mass-flowering crops are important resources for bees, but they may result in unbalanced nutrition due to different nutritional values. In this study, a semi-field experiment was conducted to evaluate the response of <em>Bombus terrestris</em> colonies after the application of a tank mixture containing the insecticide chlorantraniliprole and the fungicide prochloraz, either in monofloral-managed lupin (<em>Lupinus albus</em>) as high pollen protein resource or in presence of an additional polyfloral flower mixture. Our results demonstrate an evident effect on worker mortality after application of the tank mixture. Higher worker mortality in polyfloral treatments compared to the untreated control was observed. The number of young brood and pupae in colonies in polyfloral control were significantly higher than in monofloral treatment. However, no long-term effects on the number or weight of new queens were found. Furthermore, flowering resources, but not pesticide exposure, affected colony weight. Exposure to the tank mixture resulted in declining nectar yeasts abundance and an increasing proportion of phylloplane yeasts in forager guts. In conclusion, diverse flowering resources are important for a bumble bee colony’s development. Even in a high pollen protein crop, low flower diversity may act as an additional stressor. Thus, we suggest further maintaining and promoting flowering strips or flowering fields in agricultural landscapes, even near high pollen protein crops, to enhance bee health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118170"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of neem extract and neem oil-based azadirachtin nanopesticides for fall Armyworm control and management

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118168

Ivan Oyege , Alexi Switz , Lauren Oquendo , Anamika Prasad , Maruthi Sridhar Balaji Bhaskar

{"title":"Green synthesis of neem extract and neem oil-based azadirachtin nanopesticides for fall Armyworm control and management","authors":"Ivan Oyege , Alexi Switz , Lauren Oquendo , Anamika Prasad , Maruthi Sridhar Balaji Bhaskar","doi":"10.1016/j.ecoenv.2025.118168","DOIUrl":"10.1016/j.ecoenv.2025.118168","url":null,"abstract":"<div><div>The global spread of Fall Armyworm (FAW, <em>Spodoptera frugiperda</em>) has posed significant challenges to crop productivity and food security, with current pest management relying heavily on synthetic pesticides. This study explores the green synthesis of neem extract and neem oil-based Azadirachtin nanopesticides using cellulose acetate (CA) as a carrier polymer, focusing on their efficacy against FAW. The objective was to assess whether CA-NEP (neem extract nanopesticides) and CA-NOL (neem oil nanopesticide) formulations were effective at FAW control with minimal ecological impact. The nanopesticides were synthesized by electrospinning at concentrations of 5 %, 10 %, 20 %, 33 %, and 50 % (w/w) and characterized using Scanning Electron Microscopy and Fourier Transform Infrared spectroscopy. Azadirachtin content was quantified using Liquid Chromatography-Mass Spectroscopy. CA-NEP and CA-NOL followed first-order, and Korsmeyer-Peppas release kinetics, respectively. Feeding bioassays showed high FAW mortality rates, with 20 %-50 % CA-NEP achieving greater than 40 % mortality in less than 3 days and 50 % CA-NEP reaching 100 % mortality by day five. The mortality rates of FAW due to feeding on CA-NOL-treated corn leaves reached 40 % after 4 and 6 days, respectively, for 50 % and 33 % CA-NOL. Placing nanopesticide fibers next to corn seeds during planting significantly reduced FAW leaf damage. The lethal dose 50 (LD50) analyses showed that 13 % CA-NEP is the optimal concentration for FAW control. Environmental safety assessments on earthworms showed no acute or chronic toxicity, indicating that the nanopesticides suit ecologically sensitive areas. Therefore, these nanopesticide formulations provide a promising, eco-friendly alternative for sustainable FAW control and management with enhanced efficacy and safety.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118168"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of Raman and FTIR spectroscopy for direct microplastic search in the human milk samples: Comparative qualitative study

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118159

Agnieszka Dąbrowska , Wanda Komorowska , Balázs Kriszt , István Szabó

{"title":"Feasibility of Raman and FTIR spectroscopy for direct microplastic search in the human milk samples: Comparative qualitative study","authors":"Agnieszka Dąbrowska , Wanda Komorowska , Balázs Kriszt , István Szabó","doi":"10.1016/j.ecoenv.2025.118159","DOIUrl":"10.1016/j.ecoenv.2025.118159","url":null,"abstract":"<div><div>The ubiquitous presence of microplastic particles encompasses the human tissues and secreta. Unfortunately, the complex biological matrix hampers the proper polymer identification, and harsh purification protocols damage the microplastic particles (MPs), change the specimens frequently used and needed for additional diagnostics, and bias the final result. Moreover, purification of human milk samples is sometimes impossible, as the samples can not be subjected to any chemical pretreatment. Thus, this paper aims to check the feasibility of complementary spectral approaches, namely FTIR (Fourier-transform infrared) and Raman spectroscopy, to the fast scanning of selected MPs presence, in particular polyethylene (PE), and polystyrene (PS), in human milk samples without any previous purification to prevent the change of matrix. Although the proposed approach cannot be used for the quantitative measurement of MPs concentration or the detection of low-size fractions, it is a valuable tool for the preliminary screening of numerous population samples, and some preliminary conclusions can be drawn. One may easily detect the most common MPs and observe their eco-corona. Mapping mode is beneficial for scanning large areas. Furthermore, the spectral methods turned out to be efficient in the milk itself diagnosis, for instance, the monitoring of the fat content. The results were placed in the context of the ongoing broad discussion about MPs interaction with the human body and several possible impact mechanisms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118159"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic intervention with anti-TNF alleviates colonic and hepatic toxicity induced by perfluorooctanoic acid (PFOA)

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118125

Wei Li , Yongjing Qian , Xiaojing Cai , Yu He , Xiannan Meng , Ling Zhang

{"title":"Therapeutic intervention with anti-TNF alleviates colonic and hepatic toxicity induced by perfluorooctanoic acid (PFOA)","authors":"Wei Li , Yongjing Qian , Xiaojing Cai , Yu He , Xiannan Meng , Ling Zhang","doi":"10.1016/j.ecoenv.2025.118125","DOIUrl":"10.1016/j.ecoenv.2025.118125","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA) is a prevalent and chemically stable environmental contaminant. Our preliminary data suggest that chronic exposure to PFOA induces colonic damage in mice that resembles inflammatory bowel disease (IBD). Anti-TNF therapies are commonly used in the clinical management of IBD. Building upon our previous findings, we administered anti-TNF treatment to mice exposed to PFOA. Our results show that anti-TNF therapy significantly reduces the colonic inflammatory response, activation of the NLR family pyrin domain containing 3 (NLRP) inflammasome, and apoptosis induced by PFOA. Additionally, anti-TNF treatment restores intestinal barrier integrity, which is disrupted by PFOA exposure, and enhances the regenerative capacity of the colon by promoting intestinal stem cell function. Furthermore, anti-TNF therapy effectively mitigates hepatic inflammation, liver dysfunction, lipid metabolism disturbances, NLRP3 inflammasome activation, and apoptosis in the liver triggered by PFOA. In conclusion, our study provides compelling evidence that anti-TNF therapy can alleviate both colonic and hepatic injuries induced by PFOA exposure. This research expands our understanding of environmental toxin-induced diseases and offers potential therapeutic strategies for managing PFOA-related disorders in the future.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118125"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a quantitative analytical method for 6PPD, a harmful tire antioxidant, in biological samples for toxicity assessment

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118171

Min-Seok Choi , Sung-Hwan Kim , Moonjung Hyun , Sung Min Han , Yong-Hyun Kim

{"title":"Development of a quantitative analytical method for 6PPD, a harmful tire antioxidant, in biological samples for toxicity assessment","authors":"Min-Seok Choi , Sung-Hwan Kim , Moonjung Hyun , Sung Min Han , Yong-Hyun Kim","doi":"10.1016/j.ecoenv.2025.118171","DOIUrl":"10.1016/j.ecoenv.2025.118171","url":null,"abstract":"<div><div>This study developed and validated a high-sensitivity analytical method for the quantification of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD), a hazardous antioxidant used in tires, to support the effective management of its environmental exposure. Using an HPLC-UV system with acetonitrile and deionized water as the mobile phase, the method did not require salts or acids. The calibration curve exhibited excellent linearity (R<sup>2</sup> = 0.9999) with detection limits of 0.17 pg μL<sup>–1</sup> (LOD) and 5.51 pg μL<sup>–1</sup> (MDL), underscoring its ultra-trace detection capability. A pretreatment protocol was developed to quantify 6PPD in mouse liver samples. The protocol achieved a relative recovery rate of 71.89 % and high precision across all spiking concentrations (mean RSD = 2.20 ± 0.92 %). The validated methods were applied to analyze 6PPD residues in mouse liver following intratracheal instillation exposure. At 9 h post-exposure, residual 6PPD concentrations increased significantly in a dose-dependent manner (R<sup>2</sup> = 0.9906), with a relative distribution of 0.06 ± 0.01 %. This cost-effective and reliable method provides a practical tool for quantifying 6PPD in environmental and biological matrices. The findings enhance the understanding 6PPD exposure dynamics and support the establishment of regulatory guidelines for managing its ecological and human health risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118171"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, histopathological and brain transcriptomic assessment reveal reproductive toxicity and neurotoxicity in western mosquitofish (Gambusia affinis) exposed to levonorgestrel

IF 6.2 2区环境科学与生态学

Ecotoxicology and Environmental Safety Pub Date : 2025-04-11 DOI: 10.1016/j.ecoenv.2025.118153

Weicheng Yang , Tongfu Lu , Jian Gong , Qiang Li , Chong Han , Jianrong Huang

{"title":"Morphological, histopathological and brain transcriptomic assessment reveal reproductive toxicity and neurotoxicity in western mosquitofish (Gambusia affinis) exposed to levonorgestrel","authors":"Weicheng Yang , Tongfu Lu , Jian Gong , Qiang Li , Chong Han , Jianrong Huang","doi":"10.1016/j.ecoenv.2025.118153","DOIUrl":"10.1016/j.ecoenv.2025.118153","url":null,"abstract":"<div><div>In recent years, more and more progestogens have been detected in the environment, especially levonorgestrel (LNG), which is widely used in medicine and veterinary medicine, but its potential effects on the reproductive and nervous systems of fish are not fully understood. The purpose of this study was to investigate the reproductive and neurological effects of LNG on female western mosquitofish (<em>Gambusia affinis</em>). Through a 30-day exposure experiment, we evaluated the morphological data, gonadal and brain histopathology, and brain transcriptome of mosquitofish under different concentrations of LNG (0, 5, 50, 500 ng/L). The results revealed that exposure to LNG led to a significant reduction in the body weight and condition factor of female fish, with the most pronounced decrease observed at a concentration of 500 ng/L. Morphological observations indicated that LNG exposure led to an increase in the 4th/6th anal fin ratio at concentrations of 50 and 500 ng/L. Additionally, histopathological analysis demonstrated pathological alterations, including ovarian degeneration induced by LNG, as well as vasodilation, congestion, and the enlargement of intercellular spaces in brain tissue. Analysis of brain tissue transcriptome data identified numerous differentially expressed genes related to cerebral vascular formation, nerve injury, and neuroendocrine regulation following LNG exposure. In summary, LNG has significant reproductive and neurotoxic effects on female mosquitofish, and these findings provide important data for further research on environmental risk assessment and aquatic toxicology of LNG.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"296 ","pages":"Article 118153"},"PeriodicalIF":6.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0