Ecotoxicology and Environmental Safety最新文献

{"title":"3-acetyldeoxynivalenol3-Acetyldeoxynivalenol induces pyroptosis in leydig cells via METTL3-mediated N6-methyladenosine modification of NLRP3.","authors":"Yangyun Wang, Chaoliang Shi, Wei Jiao, Xiaoxiang Wan","doi":"10.1016/j.ecoenv.2024.117549","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117549","url":null,"abstract":"<p><p>3-acetyldeoxynivalenol (3-ADON), an acetylated derivative of deoxynivalenol, is a prevalent contaminant found in food products contaminated with mycotoxins. While the toxicological effects of 3-ADON on human and animal health are well-documented, its specific impact on the reproductive system remains underexplored. In this study, we comprehensively examined the toxicological effects of 3-ADON on TM3 Leydig cells through both in vivo and in vitro experimental models. Our results demonstrate that 3-ADON exposure leads to substantial testicular damage in vivo and significantly reduces cell viability while increasing mortality in TM3 cells in vitro (P = 0.012). Mechanistic investigations further revealed that 3-ADON exposure triggers pyroptosis in TM3 cells, as evidenced by upregulation of NLRP3, activation of caspase-1, ASC, and GSDMD. Moreover, 3-ADON treatment resulted in a significant upregulation of METTL3 expression and increased global mRNA m6A modification levels. m6A sequencing and functional assays established that METTL3-mediated m6A modification of NLRP3 mRNA enhances its stability and expression. RNA immunoprecipitation (RIP) assays further demonstrated that IGF2BP1 selectively recognizes m6A-modified NLRP3 mRNA, contributing to its stabilization. Notably, IGF2BP1 was found to inhibit the recruitment of the BTG2/CCR4-NOT complex by competitively binding to PABPC1, thereby preventing the deadenylation of NLRP3 mRNA and maintaining its expression. Additionally, we identified that METTL3 also methylates and stabilizes c-MyB mRNA, which subsequently binds to the promoter region of NLRP3, thereby enhancing its transcription. Collectively, our findings reveal a novel mechanism by which 3-ADON exerts its reproductive toxicity, underscoring the pivotal role of METTL3-mediated m6A modifications in regulating Leydig cell dysfunction.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":" ","pages":"117549"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenomethionine alleviates T-2 toxin-induced articular chondrocyte ferroptosis via the system Xc^-/GSH/GPX4 axis.","authors":"Fang-Fang Yu, Juan Zuo, Miao Wang, Shui-Yuan Yu, Kang-Ting Luo, Tong-Tong Sha, Qian Li, Zai-Chao Dong, Guo-Yu Zhou, Feng Zhang, Xiong Guo, Yue Ba, Yan-Jie Wang","doi":"10.1016/j.ecoenv.2024.117569","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117569","url":null,"abstract":"<p><p>T-2 toxin can induce bone and cartilage development disorder, and oxidative stress plays an important role in it. It is well known that selenomethionine (Se-Met) has antioxidative stress properties and promotes the repair of cartilage lesion, but it remains unclear whether Se-Met can relieve damaged cartilage exposure to T-2 toxin. Here, the oxidative stress and ferroptosis of chondrocytes exposure to T-2 toxin were observed. Mechanistically, T-2 toxin increased ROS, lipid ROS, MDA and Fe²⁺ contents in chondrocytes, decreased GSH and GPX4 activity, and inhibited the system Xc^-/GSH/GPX4 antioxidant axis. In addition, the mitochondria of chondrocytes shrunk and the mitochondrial crest decreased or disappeared. However, Fer-1 (Ferrostatin-1) inhibited ferroptosis induced by T-2 toxin in chondrocytes. The Se-Met alleviated lipid peroxidation, oxidative stress, and damaged mitochondrial in T-2 toxin-infected chondrocytes, enhanced antioxidant enzyme activity, and activated the system Xc^-/GSH/GPX4 axis, thereby antagonizing ferroptosis of chondrocytes and alleviating articular cartilage damage. In conclusion, our findings highlight the essentiality of ferroptosis in chondrocyte caused by T-2 toxin, elucidate how Se-Met offers protection against this injury and provide research evidence for the drug treatment target of Kashin-Beck disease.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"290 ","pages":"117569"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of urban green and blue space with accelerated ageing: A cohort Study in the UK Biobank.","authors":"YiNi Wang, XinYu Shi, Fei Yang, ShengYuan Wang, TianShu Han, Kun Ma","doi":"10.1016/j.ecoenv.2024.117458","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117458","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the associations between urban green and blue spaces and the incidence of accelerated ageing, which have rarely been studied.</p><p><strong>Methods: </strong>Analyzed UK Biobank data (2006-2010) on 440,785 participants, focusing on urban green and blue space (UGBS). General linear models and logistics models assessed links between UGBS and accelerated ageing (Klemera-Doubal method, telomere length and frailty). Mediation analysis examined the role of pollution and other factors.</p><p><strong>Results: </strong>After controlling for random effects and various types of covariates, we found that 1) populations with higher levels of UGBS exposure are associated with accelerated ageing and slowing down; 2) UGBS has the greatest impact on accelerated ageing within a 1000-m street network buffer distance; 3) Participants living in heavily polluted areas should pay more attention to UGBS; 4) Intermediary analysis found that UGBS mainly accelerates aging by reducing harm, rather than affecting the population's ability to recover and build.</p><p><strong>Conclusions: </strong>UGBS with a composite weighted score showed a better correlation with accelerated ageing than green spaces. Other factors such as gender, race and lifestyle also influence the impact of the environment on accelerated ageing.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":" ","pages":"117458"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of soil-groundwater system on migration and transformation of organochlorine pesticides: A review.","authors":"Haohao Li, Lin Huo, Rui Zhang, Xuefan Gu, Gang Chen, Ying Yuan, Wenbing Tan, Kunlong Hui, Yu Jiang","doi":"10.1016/j.ecoenv.2024.117564","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117564","url":null,"abstract":"<p><p>Soil is the place where human beings, plants, and animals depend on for their survival and the link between the various ecological layers. Groundwater is an important component of water resources and is one of the most important sources of water for irrigated agriculture, industry, mining and cities because of its stable quantity and quality. Soil and groundwater are important strategic resources highly valued by countries around the world. However, in recent years, the deterioration of the ecological environment of soil-groundwater caused by industrial, domestic, and agricultural pollution sources has continued to threaten human health and ecological security. Among them, organochlorine pesticides (OCPs), as typical organic pollutants, cause very serious pollution of soil and groundwater environment. However, most studies on the pollution of OCPs have focused on the aboveground or surface water environment, and little consideration has been given to the pollution and hazards of OCPs to the deep soil and groundwater environment, especially the effects of different environmental factors on the transport and transformation of OCPs in soil-groundwater. Moreover, in addition to the influence of a single factor on it, the interactions that arise between different factors cannot be ignored. This paper focuses on two major sources of OCPs in soil and groundwater environments, compiles and summarizes the effects of environmental factors such as pH, microbial communities and enzyme activities on the transport and transformation of OCPs in soil and groundwater systems, discusses the synergistic effects of individual environmental factors and others, and comprehensively analyses the effects of synergistic effects of various environmental factors on the transport and transformation of OCPs. In the context of ecological civilization construction, it provides the scientific basis and theoretical foundation for the prevention and treatment of OCPs-contaminated soil and groundwater, and puts forward new ideas and suggestions for the research and development of green, eco-friendly remediation and treatment technologies for OCPs-contaminated sites.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"290 ","pages":"117564"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway.","authors":"Chenlong Wang, Chuangjiang Chen, MengYa Wang, Sajid Ur Rahman, Bin Wei, Hongyan Ding, Wanyue Huang, Xichun Wang","doi":"10.1016/j.ecoenv.2024.117546","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117546","url":null,"abstract":"<p><p>Zearalenone (ZEA) is an environmentally widespread mycotoxin capable of posing a serious threat to food safety and public health, and porcine endometrial stromal cells (ESCs) are particularly sensitive to the toxic effects of ZEA. We hypothesized that Rutin, a flavonoid antioxidant, could significantly alleviate ZEA-induced ferroptosis through the p53 signaling pathway. In this study, we used porcine ESCs as a research model. When porcine ESCs were co-cultured with the addition of Rutin and ZEA following p53 gene silencing via siRNA transfection, Rutin significantly mitigated ZEA-induced mitochondrial damage, oxidative stress, and Fe^2 + content through the p53 pathway. Additionally Rutin lowered the expression of p53, ALOX12, and ACSL4 while significantly improving cytokinesis, antioxidant enzyme activity, and SLC7A11, GPX4, Nrf2, FTH1, thereby inhibiting cellular ferroptosis. These findings suggested a novel programmed death mechanism for alleviating the cytotoxic effects of ZEA, involving the knockdown of p53.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"290 ","pages":"117546"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occupational health risk assessment and risk monetization based on xylene exposure.","authors":"Fei Li, Boshen Wang, Baoli Zhu","doi":"10.1016/j.ecoenv.2024.117562","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117562","url":null,"abstract":"<p><p>The risk evaluation of occupational exposure to xylene was conducted to provide a basis for the formulation of policies on the prevention and control of occupational hazards of xylene and to fill the gap in this field. In this study, a number of xylene-related companies in seven industries from 2020 to 2023 were collected as research subjects, and the health risks caused by xylene exposure were evaluated using the EPA model designated by the U.S. Environmental Protection Agency, the Singapore Semi-Quantitative Risk Assessment Model (MOM), and the International Council on Mining and Metals (ICMM) Occupational Health Risk Assessment Model. Subsequently, a risk monetization method was used to convert the health risks of xylene exposure into currency. the results of the study showed that the EPA and MOM models were more reasonable and could accurately identify occupational health risks due to xylene exposure, while the ICMM model overestimated the health risks due to xylene exposure by assessing the risk level of all industries as level 4 or higher. Incorporating the number of people on the job into the risk assessment can differentiate the number of people involved in the health risks caused by xylene exposure, which is more conducive to the adoption of targeted preventive and control measures. The risk monetization model can provide a more intuitive view of the direct economic losses that may be caused by occupational exposure to xylene, which can help the relevant authorities to formulate strategies for the prevention and control of xylene hazards, and also provide a strong support for occupational damage compensation.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":" ","pages":"117562"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey on the presence of floating microplastics, trace metals and metalloids in seawater from Southern Italy to the United States of America.","authors":"Elisabetta Morici, Gaetano Cammilleri, Sergio Scirè, Francesco Paolo Bonomo, Luigi Tranchina, Francesca Terracina, Paola Galluzzo, Vincenzo Ferrantelli, Vincenzo Paolo Monteverde, Francesco Giuseppe Galluzzo, Sergio Davì, Mariano Licciardi, Salvatore Dara","doi":"10.1016/j.ecoenv.2024.117507","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117507","url":null,"abstract":"<p><p>The presence of microplastics (MPs), trace metals (TM) and metalloids (Ms) in surface seawater is a severe emerging issue of global concern. Information about the distribution of these pollutants is often lacking, and large-scale studies come with uncertainties because of difficult comparisons of results obtained using different methods to collect and process data. This study presents a comprehensive investigation of microplastics (MPs), trace metals (TM) and metalloids (Ms) in surface seawater during two transatlantic sampling campaigns, covering approximately 17,000 nautical miles. The results reveal the presence of MPs in all the samples analyzed and a broad variation in microplastic concentration (230-3320 MPs/L), with filaments or fibers being the most abundant shape. Coastal waters generally exhibit higher MPs, TM and Ms concentrations than open sea waters. The results showed high concentrations of MPs, particularly in the waters near the Faroe Islands, in the Sea of Magdalena department and in the Strait of Gibraltar. The order of the overall metals and metalloids concentrations was: As>Cr>Pb>Cd. High concentrations of Pb and Cr were recorded in the Mediterranean waters whereas high Arsenic (As) were found in the Southern coasts of United States, with values that exceeded the limits considered hazardous for aquatic life (81.55-101.12 µg/L). No significant correlations were found between microplastics, and the heavy metals examined. Here, we emphasize the need for sustainable environmental management actions and policies in a global context to monitoring the growing problem of pollutants in our oceans.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":" ","pages":"117507"},"PeriodicalIF":6.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between glyphosate exposure and cardiovascular health using \"Life's Essential 8\" metrics in US adults.","authors":"Yuankang Zhu, Zhenlin Yang, Yuhao Chen, Lingzi Qian, Changning Hao, Liangshi Hao, Boshen Yang, Junli Duan","doi":"10.1016/j.ecoenv.2024.117559","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117559","url":null,"abstract":"<p><strong>Background: </strong>Glyphosate, as one of the most widely used herbicides worldwide, has been reclassified as a potential carcinogen and linked to multiple health problems. Recent data from animal experiments have proved its potential cardiovascular toxicity. However, whether glyphosate exposure influences human cardiovascular health at the population levels remains unknown. This study aims to elucidate the correlation between glyphosate exposure and Cardiovascular Health (CVH) by utilizing comprehensive Life's Essential 8 (LE8) metrics.</p><p><strong>Methods: </strong>Data from 2842 participants, approximating 155.24 million U.S. adults, from 2013 to 2018 in National Health and Nutrition Examination Survey were analyzed. The association between natural logarithm (ln)-transformed glyphosate exposure in urine and CVH was examined using weighted linear regression and restricted cubic spline (RCS) models. Mediation analysis was used to determine potential mediators correlated with glyphosate and CVH. Further subgroup analysis and sensitive analysis were conducted to confirm the results.</p><p><strong>Results: </strong>In fully adjusted models, the total CVH score decreased by 1.33 points for every unit increase in continuous ln-transformed glyphosate [β = -1.33, 95 % confidence interval (CI) (-2.25, -0.41)]. There was a negative correlation between the total CVH score and the lowest quantiles of ln-transformed glyphosate (Q1), Q2, Q3, and Q4 with p for trend < 0.05. A non-linear relationship between glyphosate and total CVH emerged (p for non-linear<0.001, p for overall = 0.003), and no safe threshold of glyphosate was observed. Serum insulin was an important mediator in the adverse effects of glyphosate on CVH with an 18.73 % mediation proportion. Moreover, higher serum insulin levels and higher homeostasis model assessment of insulin resistance were associated with higher glyphosate exposure but negatively correlated with total CVH score.</p><p><strong>Conclusions: </strong>Glyphosate exposure may pose a risk to cardiovascular health at the population levels, with elevated serum insulin levels acting as a crucial mediating element. Further studies are required to investigate the safe threshold and underlying mechanism of glyphosate impairment.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"290 ","pages":"117559"},"PeriodicalIF":6.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos.","authors":"You Wei, Yunlong Meng, Kun Jia, Weijian Lu, Yushan Huang, Huiqiang Lu","doi":"10.1016/j.ecoenv.2024.117413","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117413","url":null,"abstract":"<p><p>Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding of the toxicological properties of DMT on these organisms. In this study, we utilized zebrafish as an animal model to assess the toxicity of DMT induced by exposure 5.5-72 hours post-fertilization (hpf). During this period, we monitored and evaluated the development of the zebrafish heart and vascular system. Additionally, embryo samples were collected to perform molecular-level detection of PCNA, oxidative stress, and related genes. The results showed a concentration-dependent decrease in survival rate and hatching rate, shortened body length, slowed heart rate, and pericardial edema, body curvature and reduced eye size as DMT exposure concentration increased. Furthermore, DMT exposure led to impairments in the development of the heart, vascular, along with change in the expression levels of relevant genes. It also caused a decrease in cell proliferation and an increase in oxidative stress levels. Moreover, DMT disrupts the normal development of thyroid follicular cells, leading to a reduction in T3 levels. Thyroid hormone supplementation partially reverses the toxicity induced by DMT, increasing eye size, restoring body length, reducing spine curvature, and reducing pericardial edema. Therefore, we speculate that DMT likely affects the development of zebrafish embryos by disrupting normal thyroid follicle development.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"289 ","pages":"117413"},"PeriodicalIF":6.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to triphenyl phosphate during pregnancy: The role of gut-bile acids-liver axis on lipid metabolism in male offspring.","authors":"Juntong Lin, Fuhui Yan, Longhui Liu, Ganzhong Liao, Yilei Xu, Qian Liu, Miaoliang Wu, Honghui Guo, Bai Zhi, Lianxian Guo, Xiaoshan Liu","doi":"10.1016/j.ecoenv.2024.117554","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2024.117554","url":null,"abstract":"<p><p>The widespread use of Triphenyl phosphate (TPhP) as a substitute flame retardant in various commercial products has raised global concerns for its health risks. Previously, we found that gestational and lactational TPhP exposure disturbed lipid metabolism and gut microbiota in offspring sex-dependently. In this study, we further explored the prenatal TPhP exposure on lipid metabolism in male offspring, and the role of gut-bile acids-liver axis in it. The results showed that gestational TPhP exposure would induce hyperlipidemia in male offspring, it dose-dependently increased the weight of body and liver, levels of serum lipid, and enlarged lipid droplets in white adipose tissue. The expression of lipid metabolism-related genes was significantly changed in the liver and adipose tissue. The gut microbiome of male offspring was also altered, with different profiles between the low and high dose treatment group. Target bile acids (BAs) metabolites analysis revealed a significant increased levels of primary BAs cholic acid (CA), but decreased levels of chenodeoxycholic acid (CDCA), and decreased levels of the secondary BAs deoxycholic acid (DCA), hyodeoxycholic acid (HDCA), ursodeoxycholic acid (UDCA), and lithocholic acid (LCA) in TPhP treatment group. Measurements of gene expression along the gut-BAs-liver axis showed that the TPhP treatment group had an increase in cholesterol-7alpha-hydroxylase (CYP7A1) and a decrease in apical sodium-dependent bile acid transporter (ASBT), with only the low-dose group exhibiting an increase in carbohydrate response element binding protein (ChREBP). Correlation and mediation analysis highlighted associations of Burkholderiaceae in low-dose treatment group and Erysipelotrichaceae in high dose group with lipid metabolism and BAs metabolites. No consistent correlations were observed in two treatment group. Additionally, Mantel tests did not reveal a consistent correlation between the microbiome network community and lipid metabolism or the gut-bile acids-liver axis. Overall, our findings indicate that gestational TPhP exposure induces hyperlipidemia in male offspring, and while the gut-BAs-liver axis plays a role, other mechanisms warrant further investigation.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"290 ","pages":"117554"},"PeriodicalIF":6.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}