Ecotoxicology and Environmental Safety最新文献

{"title":"Transcriptomics integrated with metabolomics reveals the effect of benzo[a]pyrene exposure on acute lung injury","authors":"Yuting Lin ,&nbsp;Haibo Xu ,&nbsp;Kaitao Wang ,&nbsp;Xinye Wang ,&nbsp;Xinyu Wu ,&nbsp;Zhiyi Tang ,&nbsp;Yuxi Lin ,&nbsp;Chengshui Chen ,&nbsp;Beibei Wang","doi":"10.1016/j.ecoenv.2024.117323","DOIUrl":"10.1016/j.ecoenv.2024.117323","url":null,"abstract":"<div><div>Benzo[<em>a</em>]pyrene (BaP), a major harmful component in PM2.5, is widely present in automobile emissions and atmospheric pollution. BaP exposure directly targets the lungs, often resulting in acute lung injury (ALI). However, comprehensive metabolic and transcriptomic profiles related to BaP-induced ALI remain unexplored. To simulate BaP-induced lung injury, we performed intratracheal instillation of BaP. To investigate how BaP exposure affects lung transcriptome and metabolic profiles, we used RNA sequencing and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). We aimed to understand the underlying mechanisms of BaP-induced lung damage. Metabolomics analyses indicated that in BaP-exposed animals, most fatty acids, carbohydrates, and steroids were significantly reduced, whereas most amino acids and organic acids remained unchanged. Analysis of transcriptomics data showed that fatty acid synthesis decreased and fatty acid oxidation increased, suggesting that lipid breakdown occurs after BaP exposure. Additionally, there were increases in oxidative stress system activity and decreases in immune system function. Finally, BaP altered mitochondrial, lipid, immune system, and fatty acid pathways, as indicated by pathway enrichment analyses. These results show that BaP substantially affects metabolic and inflammatory responses, enhancing the broader understanding of the underlying mechanisms of ALI after BaP exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117323"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotoxic response of ryegrass (Lolium multiflorum L.) to extreme exposure to two anionic surfactants","authors":"Emmeline D’Incau,&nbsp;Antoine Spaudo,&nbsp;Sonia Henry,&nbsp;Stéphanie Ouvrard","doi":"10.1016/j.ecoenv.2024.117320","DOIUrl":"10.1016/j.ecoenv.2024.117320","url":null,"abstract":"<div><div>Bioremediation is an effective and environment-friendly treatment used to clean up hydrocarbon-contaminated soil. However, the effectiveness of this treatment is often limited by the low bioavailability of the target contaminants. Surfactants addition thus appears as a way to increase solubility of these hydrophobic molecules and consequently improve their bioavailability. The use of biological surfactants is often favoured over synthetic ones because they are claimed to be non-toxic to the environment though few studies have addressed this issue. The present work evaluated the effects of a synthetic surfactant, sodium dodecyl sulphate (SDS) and a biosurfactant (rhamnolipids) on germination and growth of ryegrass over a wide range of concentrations, between one up to ten times their respective critical micellar concentration (CMC). Experimental results showed that SDS inhibited seed germination of <em>Lolium multiflorum</em> at high concentrations (10 × CMC), unlike rhamnolipids, which did not induce any toxicity symptom at germination stage. At the growth stage, high rhamnolipid concentrations induced chronic phytotoxicity by significantly reducing root length, decreasing biomass production and disrupting the enzymatic defence system. Thus, biosurfactants are less toxic than synthetic ones but their application at high doses in bioremediation treatments might still induce phytotoxicity symptoms and thus negatively affect the environment.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117320"},"PeriodicalIF":6.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay and long-lasting effects of maternal low-level Pb, Hg, and Cd exposures on offspring cognition","authors":"Lu Ouyang ,&nbsp;Qi Li ,&nbsp;Shuo Yang ,&nbsp;Lingyu Yan ,&nbsp;Jiajun Li ,&nbsp;Xin Wan ,&nbsp;Hui Cheng ,&nbsp;Lingling Li ,&nbsp;Peishan Liu ,&nbsp;Jie Xie ,&nbsp;Guihua Du ,&nbsp;Fankun Zhou ,&nbsp;Chang Feng ,&nbsp;Guangqin Fan","doi":"10.1016/j.ecoenv.2024.117315","DOIUrl":"10.1016/j.ecoenv.2024.117315","url":null,"abstract":"<div><div>Lead (Pb), mercury (Hg), and cadmium (Cd) are prevalent and persistent environmental contaminants, causing detrimental effects on millions of individuals worldwide. Our previous research demonstrated that early-life exposure to low-level Pb, Hg, and Cd mixtures may lead to cognitive impairments. However, the association and interaction among low levels of Pb, Hg, or Cd exposure remains unclear. In this study, a two-level full factorial design (5.481, 0.036, and 2.132 mg/L for Pb, Hg, and Cd respectively) was conducted to assess the interplay among maternal Pb, Hg, and Cd exposure on offspring cognition. Following exposure during pregnancy and lactation, a competitive absorption among Pb, Hg, and Cd was observed. Maternal exposure to each metal alone resulted in higher blood and brain concentrations of Pb, Hg, and Cd in offspring compared to co-exposure at equivalent levels. However, behavioral experiments conducted in the Morris water maze and novel object recognition test revealed maternal Pb, Hg, and Cd exposure synergistically impaired offspring's spatial cognition and recognition memory. Importantly, this dysfunction persisted into middle age even without exposure after adulthood. Moreover, the open field test and elevated plus maze indicated maternal low-level Pb, Hg, and Cd co-exposure triggered risk-taking behavior in weaning offspring, with a significant main effect for Pb exposure. No long-lasting effect on risk-taking behavior was detected in middle-aged offspring. Further investigation into molecular mechanisms showed that the dysregulation of corticosterone reaction and immune response might be the potential mechanism underlying Pb, Hg, and Cd co-exposure-induced cognitive impairments. Our study highlights the synergistic and long-lasting effects of multiple heavy metal exposures,underscoring the urgency to prevent exposure to metal mixtures among children and women of childbearing age.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117315"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical simulation and experimental study of microplastic transport under open channel shear flow: Roles of particle physical properties and flow velocities","authors":"Lulu He ,&nbsp;Jianhao Jiang ,&nbsp;Shiwei Zheng ,&nbsp;Nazhen Yu ,&nbsp;Yan Zhu ,&nbsp;Wei Han","doi":"10.1016/j.ecoenv.2024.117308","DOIUrl":"10.1016/j.ecoenv.2024.117308","url":null,"abstract":"<div><div>Microplastic (MP) transport patterns under open channel shear flow remain unclear. This study investigates the transport laws of MPs at various flow velocities, MP densities, sizes and concentrations in the U-shaped experimental flume and the numerical flume based on Lattice Boltzmann Method (LBM). The results indicate that the average horizontal particle velocity and the transport distances of Polyvinyl chloride (PVC) and Polystyrene (PS) particles increase with the average cross-sectional flow velocity, while the average vertical particle velocity decreases with it. The total average particle velocity closely matches the average vertical particle velocity, regardless of the variation in MP size, density and concentration. Formula-based analysis reveals that the acceleration of spherical MP transport mainly depends on the particle size and its consequent relative drag force term (RDFT) under the conditions with a single type of MP particles, but on the particle density and its consequent RDFT and relative gravity term (RGT) in the case concerning different types of MP particles with identical particle sizes. The average horizontal particle velocity maximum of PVC and PS are both strongly correlated with the average flow velocity maximum in the cross-section. This correlation lowers with the MP particle size and concentration, and is independent of MP density. Our findings can provide reference for the prevention and control of MP pollution in rivers.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117308"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics reveals the metabolic disturbance caused by arsenic in the mouse model of inflammatory bowel disease","authors":"Yanmei Wu ,&nbsp;Yin Wang ,&nbsp;Yiling Lin ,&nbsp;Xiang Zhong ,&nbsp;Yuxian Liu ,&nbsp;Yanpeng Cai ,&nbsp;Jingchuan Xue","doi":"10.1016/j.ecoenv.2024.117305","DOIUrl":"10.1016/j.ecoenv.2024.117305","url":null,"abstract":"<div><div>Arsenic exposure has long been a significant global health concern due to its association with various human diseases. The adverse health effects of arsenic can be influenced by multiple factors, resulting in considerable individual variability. Individuals with inflammatory bowel disease (IBD) are particularly vulnerable to the effects of toxin exposure, yet the specific impact of arsenic in the context of IBD remains unclear. In this study, we employed a non-targeted metabolomics approach to investigate how arsenic exposure affects metabolic homeostasis in an IBD model using <em>Helicobacter trogontum</em>-infected interleukin-10 deficient mice. Our results demonstrated that arsenic exposure disrupted the balance of various metabolites, including tryptophan, polyunsaturated fatty acids, purine and pyrimidine metabolites, and branched-chain amino acids, in mice with colitis but not in those without colitis. Notably, several crucial metabolites involved in anti-inflammatory responses, oxidative stress, and energy metabolism were significantly altered in mice with colitis. These results indicate that arsenic exposure in an IBD context can lead to extensive metabolic disturbances, potentially exacerbating disease severity and impacting overall health. This study underscores the necessity of evaluating arsenic toxicity in relation to IBD to better understand and mitigate associated health risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117305"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated approach to evaluating water contaminants and evaporation in agricultural water distribution systems","authors":"Chloe L. Fender ,&nbsp;Stephen P. Good ,&nbsp;Manuel Garcia-Jaramillo","doi":"10.1016/j.ecoenv.2024.117277","DOIUrl":"10.1016/j.ecoenv.2024.117277","url":null,"abstract":"<div><div>This study presents an innovative approach for assessing water quality in agricultural irrigation networks, integrating stable isotope analysis, <em>in vivo</em> zebrafish screening, and comprehensive chemical profiling to investigate the occurrence, transformation, and potential toxicity of organic contaminants. Stable isotope analysis was used to measure evaporation as a proxy for water residence time in the canal, while liquid chromatography-high resolution mass spectrometry (LC-HRMS) identified a range of organic compounds in water samples collected from both the irrigation canal and its source river. Results indicated a reduction in contaminant levels in the canal compared to the river, with the most significant evaporation and concentration changes occurring at a holding reservoir, suggesting that managing residence time could help reduce water loss in arid irrigation networks. The data also highlighted how evaporation, particularly during the dry, hot season, influences contaminant dynamics. Hierarchical clustering of LC-HRMS results showed notable differences between the chemical profiles of canal and river samples, indicating that irrigation systems may contribute to the degradation or removal of certain compounds. Over 60 % of detected compounds were naturally derived, with anthropogenic contaminants like pesticides and personal care products further highlighting human impacts. Priority contaminants, including DEET and 2-naphthalene sulfonic acid, likely originated from urban activities upstream. Initial screening using zebrafish embryos showed bioactivity across sites, confirming the presence of contaminants needing further examination. Correlation analysis linked natural compounds to evaporation rates, suggesting that flora and fauna play significant roles in the chemical makeup of canal water. Overall, this approach provides a comprehensive framework for monitoring irrigation water, offering insights into contaminant behavior and supporting the development of standardized methods for assessing chemical fate and ecological risks in agricultural irrigation systems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117277"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrast of hydraulic conductivity induces transport of combined pollutants in high- and low-permeability systems","authors":"Zhongran Wu ,&nbsp;Shengyu Wu ,&nbsp;Yao Hou ,&nbsp;Min Zhang ,&nbsp;Jianhong Liang ,&nbsp;Chao Cai","doi":"10.1016/j.ecoenv.2024.117297","DOIUrl":"10.1016/j.ecoenv.2024.117297","url":null,"abstract":"<div><div>The transport process of pollutants in the environment can be influenced by heterogeneous geologic architecture and pollutant interactions. However, there has been a lack of research on co-transport behaviors of combined pollutants in heterogeneous aquifers. In this study, a series of two-dimensional tank experiments were carried out to study the transport behavior of toluene and naphthalene in both homogeneous and heterogeneous aquifers. The results revealed that the coexisting solutes facilitated the transport of toluene and naphthalene in the homogeneous aquifers, potentially due to competitive adsorption between these compounds. In the high- and low-permeability systems, the transport rates for both toluene and naphthalene decreased while exhibiting characteristics such as early arrival, long tails, and multiple peaks. The spatial analysis of pollutant distribution indicated that hydraulic conductivity contrast played a critical role in inducing back diffusion phenomenon. Furthermore, toluene exhibited more pronounced matrix diffusion compared to naphthalene in heterogeneous aquifers, characterized by higher concentrations, wider diffusion range in low-permeability zones. And the β value for toluene is smaller than naphthalene in CTRW model, indicating that the former is more sensitive to the hydraulic conductivity contrast. This study provides novel insights into understanding the co-transport behavior of combined pollutants in heterogenous aquifers.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117297"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between maternal exposure to polycyclic aromatic hydrocarbons and birth anthropometric outcomes: A systematic review and meta-analysis","authors":"Ali Mohammad Latifi ,&nbsp;Fatemeh Abdi ,&nbsp;Mohammad Miri ,&nbsp;Sara Ashtari ,&nbsp;Seyedeh Noushin Ghalandarpoor-attar ,&nbsp;Milad Mohamadzadeh ,&nbsp;Abbas Ali Imani Fooladi ,&nbsp;Shahab Uddin ,&nbsp;Amir Vahedian-azimi","doi":"10.1016/j.ecoenv.2024.117290","DOIUrl":"10.1016/j.ecoenv.2024.117290","url":null,"abstract":"<div><h3>Background</h3><div>Previous research has proposed that exposure to polycyclic aromatic hydrocarbons (PAHs) during pregnancy could potentially lead to a higher risk of adverse birth anthropometric outcomes. However, the current evidence on this connection remains inconclusive, as various studies have presented conflicting results.</div></div><div><h3>Objective</h3><div>This systematic review and meta-analysis seeks to synthesize the available research on the potential link between maternal PAHs exposure and birth anthropometric outcomes.</div></div><div><h3>Methods</h3><div>A comprehensive search of Scopus, PubMed/MEDLINE, Web of Science, and the Cochrane Library up to July 1, 2024, was conducted to identify studies investigating the impact of maternal PAHs exposure during pregnancy on birth anthropometric measures, including small gestational age (SGA), low birth weight (LBW), birth weight (BW), birth length (BL), birth head circumference (BHC), and birth chest circumference (BCC). Quality assessment was performed using the Newcastle-Ottawa Scale (NOS) and the GRADE framework, and a random-effects meta-analysis was conducted to consolidate association estimates.</div></div><div><h3>Results</h3><div>Out of 5499 articles initially screened, 27 studies were included in the review. The meta-analysis revealed no significant association between maternal PAHs exposure and LBW (OR: 1.02, 95 % CI: 0.96–1.08), with moderate heterogeneity (I²: 25.8 %, P=0.37). Notably, PAHs exposure was significantly associated with BW (β: −9.79 g, 95 % CI: −16.71 to −2.87), along with high heterogeneity (I²: 99.9 %, P&lt;0.001), and shorter BL (β: −0.04 cm, 95 % CI: −0.07 to −0.01), also with high heterogeneity (I²: 84.3 %, P&lt;0.001). Additionally, a borderline significant decrease in BHC was observed (β: −0.01 cm, 95 % CI: −0.02 to −0.00) with no significant heterogeneity among studies. The results SGA were inconsistent across the studies.</div></div><div><h3>Conclusion</h3><div>Maternal exposure to PAHs was associated with adverse birth anthropometric outcomes, particularly lower BW and BL. The borderline significant reduction in BHC suggests a potential impact worth further investigation, although this finding remains inconclusive and not yet actionable. Results for SGA varied significantly among studies, underscoring the complexity of these associations. Collectively, these findings highlight the necessity for additional research to elucidate the effects of specific PAH metabolites on birth anthropometric outcomes and to explore potential interventions aimed at mitigating the identified risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117290"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel method for real-time inhalation toxicity assessment in mice using respirometric system: A promising tool for respiratory toxicology","authors":"Yu-Yeong Choi ,&nbsp;Fida Hussain ,&nbsp;So-Yeon Kim ,&nbsp;Ho Jung Bae ,&nbsp;Ju-Yeon An ,&nbsp;Hyun-Jeong Kim ,&nbsp;Ye Eun Cho ,&nbsp;So-Young Cho ,&nbsp;Ji Woong Choi ,&nbsp;Sang-Eun Oh ,&nbsp;Se Jin Park","doi":"10.1016/j.ecoenv.2024.117333","DOIUrl":"10.1016/j.ecoenv.2024.117333","url":null,"abstract":"<div><div>Inhalation toxicity assessment is a crucial tool for the identification and classification of hazardous materials like volatile organic carbons, aerosols, and particulate matter. Unlike traditional acute inhalation toxicity studies that use mortality as an endpoint, the Fixed Concentration Procedure (FCP) emphasizes \"evident toxicity\" by monitoring behavior, weight, and food intake. This reduces reliance on mortality but doesn't directly address respiratory system impact. The present study introduced a respirometer-based inhalation toxicity and respiratory status assessment method. The toxicity evaluation system integrated a respirometric system with an animal exposure chamber, enabling real-time monitoring of oxygen consumption. The ICR mice were exposed to various concentrations of benzene (10, 20, 40, and 80 mg/L of air), toluene (7.5, 15, 30, and 60 mg/L of air), and xylene (7.5, 15, 30, and 60 mg/L of air). The respiration rate decreased by 70 % and 69 % for benzene (80 mg/L of air) and toluene (60 mg/L of air), respectively, with EC₅₀ values of 32.5 mg/l and 21.2 mg/L based on oxygen consumption. Xylene did not exhibit EC₅₀ values at the tested concentrations. However, the oxygen consumption rate significantly decreased (46 %) at high concentrations (60 mg/L of air), indicating sub-lethal toxicological effects. Furthermore, the present study was also validated in the bleomycin-induced idiopathic pulmonary fibrosis (IPF) model, demonstrating its reliability as a respiratory impairment marker. The results exhibited a strong correlation between weight loss and less oxygen consumption in the BLM group (bleomycin-induced) as compared to the SHAM group (control), which was confirmed by histological examination and protein marker analysis. The results suggest the potential use of oxygen consumption as an endpoint measurement in inhalation toxicity assessment tests without animal sacrifice, and the present study could be useful for providing valuable insights into disease progression and pharmacological interventions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117333"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to perfluorodecanoic acid impairs follicular development via inducing granulosa cell necroptosis","authors":"Zekun Liu ,&nbsp;Zhenyan Cui ,&nbsp;Chunming Li ,&nbsp;Kean Lu ,&nbsp;Kelie Chen ,&nbsp;Wei Cui ,&nbsp;Yihua Wu ,&nbsp;Dajing Xia","doi":"10.1016/j.ecoenv.2024.117268","DOIUrl":"10.1016/j.ecoenv.2024.117268","url":null,"abstract":"<div><div>Per- and polyfluoroalkyl substances (PFAS) have attracted significant attention due to their environmental toxicity. However, the detrimental impact of PFAS on the development of the female reproductive system remains controversial. In this study, we investigated the effects of three specific PFAS compounds perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA) on ovarian development. Among these compounds, PFDA demonstrated the most pronounced cytotoxic effect on ovarian granulosa cells. The results showed that a 200 μM concentration of PFDA induced cell apoptosis via the intrinsic pathway by elevating reactive oxygen species (ROS) levels and activating Caspase-9 and Caspase-3. Furthermore, 200 μM PFDA triggered necroptosis, a form of regulated cell death (RCD), through the receptor-interacting serine/threonine kinase 1 (RIPK1), receptor interacting protein kinase 3 (RIPK3), and mixed-lineage kinase domain-like protein (MLKL) axis, mediated by inhibition of the canonical apoptosis proteolytic enzyme Caspase-8. In vivo experiments confirmed that mice exposed to PFDA displayed a significantly reduced ovarian index compared to the control group, accompanied by evident follicular atresia. Ovarian tissues from the PFDA-exposed group showed upregulated necroptosis markers, which were effectively mitigated by inhibiting the phosphorylation of RIPK1 at Ser166. Importantly, this study provides the first evidence that PFDA disrupts ovarian development through a novel mechanism involving the RIPK1-mediated necroptosis pathway, alongside the detection of the intrinsic apoptosis pathway. This greatly expands our insight into the effects of PFDA on cell death. This finding highlights the potential public health hazards associated with PFDA exposure and emphasizes the need for further research to fully understand its broader implications.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117268"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}