Ecotoxicology and Environmental Safety最新文献

{"title":"Simultaneous removal and optical determination of mercury ions by constructing fluorescent recombinant T. thermophila SB210 strains","authors":"Shu-Hui Zhao ,&nbsp;Kai-Kang Hu ,&nbsp;Jie Xiong ,&nbsp;Jia-Wei Tu ,&nbsp;Wei Miao","doi":"10.1016/j.ecoenv.2025.118325","DOIUrl":"10.1016/j.ecoenv.2025.118325","url":null,"abstract":"<div><div>Removing and monitoring Hg²⁺ ions in the pollution sources based on microorganisms have the advantages of low cost and convenience. To reduce the harm of Hg²⁺ ions to humans and ecosystems in the environment, it is considered very meaningful to establish a biological treatment method using protozoa to simultaneously detect and remove Hg²⁺ ions in polluted water. In this work, the coding region of <em>MTT1</em> and <em>MTT5</em> genes in <em>Tetrahymena thermophila</em> SB210 (<em>T. thermophila</em> SB210) was replaced with green fluorescence protein (<em>GFP</em>) gene using genetic manipulation techniques. Fluorescent recombinant <em>T. thermophila</em> SB210 strains MTT1-GFP and MTT5-GFP were constructed, and the relationship between MTT1-GFP and MTT5-GFP and the effect of simultaneous response and removal of Hg²⁺ ions was explored. The knockout of <em>MTT1</em> and <em>MTT5</em> genes and introduction of <em>GFP</em> significantly reduced cell tolerance to Hg²⁺ ions, slightly increased the removal rate of Hg²⁺ ions, and induced fluorescence response to Hg²⁺ ions. Using MTT1-GFP at a cell density of 150 × 10⁴ cells mL⁻¹, the removal rate of Hg²⁺ ions were over 90 % at range 6000 μg L⁻¹ to 10000 μg L⁻¹ in 12 h. The limit of detection (LOD) was 1.063 μg L⁻¹ and the linear detection range of Hg²⁺ concentration was 200–8000 μg L⁻¹ using MTT1-GFP as fluorescence reporter. In addition, the different functional effects of MTT1-GFP and MTT5-GFP on Hg²⁺ and Cd²⁺ were compared. This study will expand our understanding of the role of <em>MTT1</em> and <em>MTT5</em> genes and provide a method for simultaneous response and removal of Hg²⁺ ions in water bodies using <em>T. thermophila</em>.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118325"},"PeriodicalIF":6.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollution is ubiquitous: Community-based quantitative human health risk assessment of metal(loid) exposure from contaminated garden plants and soils","authors":"Kunal Palawat ,&nbsp;Mónica D. Ramírez-Andreotta","doi":"10.1016/j.ecoenv.2025.118314","DOIUrl":"10.1016/j.ecoenv.2025.118314","url":null,"abstract":"<div><div>Communities across the world are attempting to practice food sovereignty, yet metal(loid) contamination of gardens may restrict one’s right to define their own relationship to the environment. To understand the human health risks associated with growing and eating plants in environmental justice communities, we aggregated environmental monitoring data at 231 sites from 10 participatory research projects. Using a Bayesian quantitative risk assessment, we simulated cancer and non-cancer health risks of ingesting garden soil and crops, and simulated a comparison to the U.S Food and Drug Administration's Total Diet Study (TDS) of conventionally-grown produce. Median hazard index (HI) – (sum of risks from 17 analytes and 20 exposure routes) - and increased excess lifetime cancer risks (IELCR) – (4 analytes, 20 exposure routes) - were 2.38 and 6.53 × 10⁻⁴ (6.53 out of 10,000) respectively. Key findings were that arsenic, manganese, copper, and barium contributed most to HI, at 29 %, 19 %, 11 %, and 9 % contribution respectively, while arsenic contributed most to IELCR at 95 % contribution. Collard greens, kale, turnip, radish, berries, string beans, cucumber, and squash had the highest risks. Community risks were slightly higher than TDS risks, except for nickel IELCRs. Consuming either home-grown produce or TDS produce poses substantial health risks. However, home-grown produce also contained higher levels of nutrients than TDS produce. Potential health benefits from gardening and eating home-grown produce, as well as an anti-colonial exposure assessment should be studied further. Individual action is not enough to protect human health; root causes of contamination such as industrialization and colonization must be addressed.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118314"},"PeriodicalIF":6.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of fluorinated substituents and Fe(Ⅱ) on ferrihydrite transformation and antibiotic degradation","authors":"Liting Wang ,&nbsp;Shikun Wei ,&nbsp;Liwen Zhang ,&nbsp;Zhiyong Guo ,&nbsp;Yan Lei ,&nbsp;Deming Dong","doi":"10.1016/j.ecoenv.2025.118313","DOIUrl":"10.1016/j.ecoenv.2025.118313","url":null,"abstract":"<div><div>Ferrihydrite is commonly associated with antibiotics in natural environments due to its strong sorption capabilities and high specific surface area. Under reducing conditions, Fe(II) acts as a catalyst for the transformation of ferrihydrite into more crystalline minerals. However, the influence of antibiotic molecular structure on the Fe(II)-catalyzed transformation of ferrihydrite and the associated degradation mechanisms of antibiotics have remained unclear. This study employed enoxacin (ENO), a representative fluorinated pharmaceutical, and pipemidic acid (PPA), a structural analog of ENO, to investigate the effect of fluorinated substituents on Fe(II)-facilitated ferrihydrite transformation. The results revealed that the transformation of ferrihydrite in the ENO system was 2.8 times greater than in the PPA system. ENO degradation reached 74.3 %, which was 1.13 times higher than that of PPA. ENO degradation products were more prone to hydroxylation, decarboxylation, and piperazine ring oxidation, whereas PPA degradation primarily involved oxidation of the piperazine ring. The fluorinated substituent in ENO facilitated ferrihydrite transformation by influencing the concentration of adsorbed Fe(II) and the distribution of antibiotics within the mineral inside. Furthermore, the fluorinated substituent in ENO enhanced degradation by increasing electron transfer between ENO and Fe(III), raising the content of adsorbed Fe(II) and promoting the formation of goethite. Collectively, these findings provide new insights into the environmental behavior of ferrihydrite and the fate of structurally different antibiotics in natural systems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118313"},"PeriodicalIF":6.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioselective toxicity of (S)-amlodipine towards zebrafish embryos: Abnormal ocular development, cardiac dysfunction, and malformed intersegmental vessels","authors":"Chaeeun Kim ,&nbsp;Donghyeon Kim ,&nbsp;Sung-Eun Lee","doi":"10.1016/j.ecoenv.2025.118315","DOIUrl":"10.1016/j.ecoenv.2025.118315","url":null,"abstract":"<div><div>Amlodipine (AM), widely used for the treatment of hypertension and angina, consists of two enantiomers: (<em>S</em>)-AM and (<em>R</em>)-AM. The extensive use of this medication has raised concerns regarding environmental contamination, but the enantioselective toxicity of AM in aquatic organisms has not been adequately investigated. This study aimed to examine the enantioselective toxicity of AM using zebrafish (<em>Danio rerio</em>) embryos, focusing on acute toxicity and developmental toxicity. Neither enantiomer exhibited acute toxicity at the tested concentrations (0.25–20 mg/L). With respect to ocular development, (<em>R</em>)-AM reduced eye volume at the highest tested concentration. The lens-to-eye volume ratio showed a significant increase in embryos treated with both enantiomers compared to the control. Enantioselective toxicity was evident in cardiac function, as (<em>S</em>)-AM-induced heart dysfunction, despite both enantiomers displaying similar patterns of heart development-related gene expression. Severe defects in ISV formation were observed in both (<em>S</em>)-AM and (<em>R</em>)-AM treatments, indicating that AM exposure may result in abnormal blood vessel formation in other fish. Metabolomic analysis indicated that exposure to either enantiomer led to the upregulation of most metabolic pathways, except for starch and sucrose metabolism. Further research needs to confirm enantioselective toxicity under chronic conditions at environmentally relevant concentrations of AM.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118315"},"PeriodicalIF":6.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lifetime co-exposure of parental zebrafish to benzophenone-3 and titanium dioxide nanoparticles leads to developmental neurotoxicity and thyroid endocrine disruption in their offspring","authors":"Linxuan Tian ,&nbsp;Chunlan Dai ,&nbsp;Enfu Feng ,&nbsp;Qinyuan Yang ,&nbsp;Yao Jiang ,&nbsp;Yubo Liu ,&nbsp;Yanni Wang ,&nbsp;Junyan Tao","doi":"10.1016/j.ecoenv.2025.118343","DOIUrl":"10.1016/j.ecoenv.2025.118343","url":null,"abstract":"<div><div>Benzophenone-3 (BP3) is a prevalent environmental UV filter widely used in personal care products, and titanium dioxide nanoparticles (nano-TiO₂), another commonly applied material in consumer goods and industrial applications, may coexist with BP3 in environmental media. This study investigates the potential parental transfer of BP3 and transgenerational effects on development and thyroid hormone homeostasis in F1 larvae following lifetime parental exposure to BP3 and nano-TiO₂. Zebrafish embryos were exposed to environmentally relevant concentrations of BP3 (10 μg/L), nano-TiO₂ (100 μg/L), and their combination from 6 hours post-fertilization (hpf) to 150 days. Results showed the presence of BP3 in the gonads of F0 and F1 embryos, with combined exposure alleviating BP3 accumulation. Parental BP3 exposure increased BP3 levels in F1 embryos, causing various developmental neurotoxic effects including decreased survival rates, somite counts, hatching rates, midbrain-hindbrain junction abnormalities, and heightened locomotor responses in F1 offspring. These effects were accompanied by their reduced axonal growth, impaired neurogenesis, and altered neurotransmitters levels. Additionally, decrease thyroxin (T4) levels were observed in F1 eggs, consistent with F0 adults’ plasma levels, indicating maternal transmission of thyroid endocrine disruption to the offspring. Furthermore, significant changes in the expression of genes related to hypothalamic-pituitary-thyroid (HPT) axis were observed across two generations, potentially contributing to transgenerational thyroid hormone disruption. Taken together, our study illustrated that parental exposure to BP3 and nano-TiO₂ can induce developmental neurotoxicity and thyroid endocrine disruption in offspring, emphasizing the importance of conducting transgenerational toxicity tests for assessing the environmental risks associated with co-exposure to UV filters and nanoparticles.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118343"},"PeriodicalIF":6.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Independent and combined effects of volatile organic compounds on sarcopenia: Insights into environmental pollutants and muscle health","authors":"Haobiao Liu ,&nbsp;Zhuohang Chen ,&nbsp;Rongqi Xiang ,&nbsp;Yiting Liu","doi":"10.1016/j.ecoenv.2025.118344","DOIUrl":"10.1016/j.ecoenv.2025.118344","url":null,"abstract":"<div><div>This study investigates the associations between volatile organic compound (VOC) exposure and sarcopenia, focusing on independent and combined effects and exploring potential biological mechanisms. Utilizing data from the National Health and Nutrition Examination Survey, we applied logistic regression to examine associations between individual VOCs and sarcopenia, while restricted cubic splines (RCS) assessed dose-response relationships. Weighted quantile sum (WQS) and Bayesian kernel machine regression (BKMR) evaluated mixed exposure effects. Mediation analyses explored the roles of potential mediators, alongside bioinformatic analyses to investigate the underlying mechanisms. Multivariable logistic regression identified significant associations between specific VOCs and sarcopenia, particularly for DHBMA (OR=2.71, 95 % CI: 1.62–4.55). RCS confirmed both linear and nonlinear associations of specific VOCs. WQS analysis corroborated a synergistic effect of mixed VOC exposures and increased sarcopenia risk (OR=1.64, 95 % CI: 1.14–2.36), and BKMR analysis further confirmed this positive relationship. Mediation analysis revealed that inflammation, oxidative stress, and renal function partially mediated these associations (mediated proportions: 4.67–11.00 %). Bioinformatic analyses highlighted apoptosis-related targets and pathways as key mechanisms underlying the observed associations. This study provides comprehensive evidence linking VOC exposure to sarcopenia, emphasizing the importance of reducing VOC exposure to prevent sarcopenia and associated health risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118344"},"PeriodicalIF":6.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of insecticide resistance mechanisms in field populations of the bean flower thrips, Megalurothrips usitatus (Bagnall)","authors":"Hongyi Cao ,&nbsp;Jiangjiang Yuan ,&nbsp;Yanran Wan ,&nbsp;Yingxi Tang ,&nbsp;Xiaobin Zheng ,&nbsp;Jing Wang ,&nbsp;Kanghua Qian ,&nbsp;Jiuming Feng ,&nbsp;Sirui Chen ,&nbsp;Youjun Zhang ,&nbsp;Qingjun Wu","doi":"10.1016/j.ecoenv.2025.118316","DOIUrl":"10.1016/j.ecoenv.2025.118316","url":null,"abstract":"<div><div>The bean flower thrips, <em>Megalurothrips usitatus</em> (Bagnall), has caused significant damage to leguminous crops in the Asian tropics and established populations in North America. Ineffective pest control has been reported in multiple regions, raising concerns about food safety risks due to improper insecticide use. To evaluate insecticide susceptibility and the role of detoxification enzyme involvement, resistance monitoring and enzyme activity assays were conducted. RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) techniques validated key gene functions. Results showed that the XW population was resistant to five insecticides, with resistance ratios ranging from 7.17 to 42.90 compared to the reference population. The NJ and YZ populations showed resistance to two and one insecticide, respectively, while the HP population was most susceptible. Elevated cytochrome P450s (CYP450), UDP-glycosyltransferases, and acetylcholinesterases activities were observed in the YZ population. Three CYP450 genes positively correlated with CYP450 activity. Carboxylesterases (CarE) activity was the highest in the XW population, with <em>MusiPT_006325</em> positively correlated and <em>MusiPT_006095</em>/<em>MusiPT_003750</em> negatively correlated with CarE activity. Glutathione-S-transferase activity was the highest in the HP population. WGCNA revealed positive correlations between MEblue, MEturquoise, and MEbrown modules and acetamiprid/ novaluron median lethal concentration (LC₅₀) values, but negative correlations with β-cypermethrin, spinetoram, emamectin benzoate, thiamethoxam, and spirotetramat LC₅₀ values. Ten CYP450 genes and three UGT genes were identified as hub genes. Our findings reveal insecticide resistance and the role of detoxification enzymes in <em>M. usitatus</em>, providing valuable insights for developing effective management strategies against this thrips species.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118316"},"PeriodicalIF":6.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polystyrene nanoplastics aggravate house dust mite induced allergic airway inflammation through EGFR/ERK-dependent lung epithelial barrier dysfunction","authors":"Qing Wang ,&nbsp;Wen He ,&nbsp;Yufeng Zhou ,&nbsp;Rui Feng ,&nbsp;Yingwen Wang ,&nbsp;Lijuan Liu ,&nbsp;Yuan Yuan ,&nbsp;Jiajia Dai ,&nbsp;Yun Liu ,&nbsp;Xiaobo Zhang","doi":"10.1016/j.ecoenv.2025.118329","DOIUrl":"10.1016/j.ecoenv.2025.118329","url":null,"abstract":"<div><div>Concerns that airborne micro- and nanoplastics (MNPs) may impair human respiratory health are rising. However, the specific effects of MNPs on allergic asthma remain insufficiently explored. This study developed an allergic asthma model using house dust mite (HDM), and mice were exposed to 50 μg polystyrene nanoparticles (PS-NPs) at three-days interval. Additionally, the effects and potential mechanisms of PS-NPs exposure (25, 50 and 100 μg/mL) on lung epithelial barrier dysfunction were explored using mouse lung epithelial type II (MLE-12) and A549 cells. The pathological changes of airway tissue and the increase of inflammatory response confirmed that exposure to PS-NPs significantly aggravated allergic asthma in mice. Importantly, in the presence of HDM sensitization, the accumulation of PS-NPs in the alveolar region was increased, leading to lung epithelial barrier dysfunction and more Th2-mediated eosinophilic inflammation, characterized by elevated IL-4, IL-13, immunoglobulin E (Ig E) and eosinophils. The activation of the epidermal growth factor receptor (EGFR) pathway and its downstream extracellular regulating kinase (ERK) was investigated using transcriptomic sequencing to elucidate the effects of PS-NPs exposure on lung epithelial barrier dysfunction. Furthermore, an EGFR-specific inhibitor AG1478 was employed to confirm the role of the EGFR/ERK pathway in lung epithelial barrier dysfunction and asthma exacerbation in vitro and in vivo experiments. In conclusion, the molecular mechanism by which PS-NPs aggravates asthma in mice was elucidated, which helps to improve the understanding of the health effects of PS-NPs and lays a theoretical foundation for addressing the health risks posed by PS-NPs.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118329"},"PeriodicalIF":6.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terrestrial iron sulfide minerals induce distinct regulation of intracellular redox homeostasis and iron assimilation","authors":"Qinying Lyu ,&nbsp;Yui Kouketsu ,&nbsp;Akira Tazaki ,&nbsp;Masashi Kato ,&nbsp;Yashiro Motooka ,&nbsp;Shinya Toyokuni","doi":"10.1016/j.ecoenv.2025.118327","DOIUrl":"10.1016/j.ecoenv.2025.118327","url":null,"abstract":"<div><div>Repeated exposure to airborne terrestrial natural minerals may cause pneumoconiosis and lung cancer, among which iron sulfide is identified as an aggravating factor. In the biological system, iron-sulfur cluster is an inorganic cofactor that is evolutionarily conserved in all the living organisms. Whereas ferrous iron catalyzes the generation of hydroxyl radicals, sulfur is indispensable as a component of antioxidants, such as glutathione. Imbalanced redox homeostasis contributes to oxidative stress, causing ferroptosis, an iron-dependent regulated necrosis characterized by lipid peroxidation, resulting in various disorders. We undertook this study to understand the cellular regulatory mechanisms against major terrestrial minerals containing iron and sulfur from the viewpoint of cellular redox. We used fundamental iron sulfide minerals collected from natural sources to treat human macrophage and fibroblast cells and investigated the biological responses. Alterations in sulfane sulfur, glutathione and iron have been analyzed using either specific fluorescent probes or inductively coupled plasma mass spectrometry. Iron sulfide microparticles with high Fe/S ratio (pyrrhotite; Fe_1-XS) induced more reactive sulfane species and glutathione, with less catalytic iron inside cells, whereas the mineral with low Fe/S ratio (pyrite; FeS₂) exhibited the opposite effects. Notably both showed cytotoxicity, where pyrite caused ferroptosis but pyrrhotite led to non-ferroptotic disruption. Furthermore, assimilated cellular excess iron was secreted via CD63(+) exosome containing iron-loaded ferritin to the extracellular space with higher iron content in pyrrhotite. Our findings suggest that iron and sulfur work complementarily in maintaining intracellular redox homeostasis, which would be crucial to understand the associated pathology.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118327"},"PeriodicalIF":6.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth","authors":"Shaokai Liu,&nbsp;Xueying Liu,&nbsp;Donghan Wang,&nbsp;Junjie Li,&nbsp;Yanshen Fu,&nbsp;Chunyang Guo,&nbsp;Huilin Tang,&nbsp;Junheng Gao,&nbsp;Xiangqun Yuan,&nbsp;Yiping Li","doi":"10.1016/j.ecoenv.2025.118322","DOIUrl":"10.1016/j.ecoenv.2025.118322","url":null,"abstract":"<div><div>While antibiotics are occasionally used in orchard bacterial disease management, their ecological impacts remain understudied. This investigation reveals significant sublethal effects of norfloxacin on the key fruit tree pest <em>Grapholita molesta</em> (oriental fruit moth). Our results showed that the antibiotic norfloxacin induced multiple developmental alterations by reducing the abundance of Enterobacteriaceae bacteria in the larval guts and adult ovaries: larval stage prolongation (+8.64 %), reduced pupal weight (-6.95 %), shortened adult period (-9.55 %), and impaired female fecundity with 24.77 % lower egg production and 16.57 % reduced egg hatchability. Our findings demonstrate that optimized antibiotic applications could simultaneously manage bacterial pathogens and suppress pest populations, providing dual agricultural benefits. This study proposes a novel integrated approach for orchard management through targeted antibiotic utilization.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118322"},"PeriodicalIF":6.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}