Ecotoxicology and Environmental Safety最新文献

{"title":"When crayfish face painkillers: Tissue-specific cytotoxic and oxidative responses to indomethacin exposure in Procambarus clarkii","authors":"Federica Impellitteri ,&nbsp;Camilla Mossotto ,&nbsp;Annalisa Cotugno ,&nbsp;Alessandra Maganza ,&nbsp;Francesca Provenza ,&nbsp;Alice Gabetti ,&nbsp;Giuseppe Esposito ,&nbsp;Monia Renzi ,&nbsp;Valerio Matozzo ,&nbsp;Antonia Concetta Elia ,&nbsp;Caterina Faggio ,&nbsp;Marino Prearo ,&nbsp;Paolo Pastorino","doi":"10.1016/j.ecoenv.2025.119138","DOIUrl":"10.1016/j.ecoenv.2025.119138","url":null,"abstract":"<div><div>Non-steroidal anti-inflammatory drugs (NSAIDs) are increasingly recognized as emerging contaminants in freshwater ecosystems. However, their effects on aquatic invertebrates remain poorly investigated. This study evaluated for the first time both cellular and biochemical responses of the red swamp crayfish (<em>Procambarus clarkii</em>) following a long-term exposure to indomethacin, a widely used NSAID. Specimens were exposed for 28 days to 2.5, 5, and 10 mg/L, and biomarkers indicative of cytotoxicity and oxidative stress were evaluated. Viability of both hemolymph and hepatopancreas cells was assessed using Trypan Blue and Neutral Red assays, while oxidative stress biomarkers, including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and malondialdehyde (MDA), were analyzed in hepatopancreas, gill, and muscle tissues. The results showed that indomethacin exposure induced significant, tissue-specific cytotoxic and oxidative responses. Notably, hepatopancreas cell viability decreased significantly at lower concentrations, with partial recovery at the highest dose, suggesting a possible hormetic effect of the contaminant. Oxidative stress biomarkers exhibited concentration-dependent changes: SOD and MDA levels were significantly altered in the hepatopancreas; GST activity was affected in the gills, and GPx activity increased in muscle at higher concentrations. Haemocyte viability remained unaffected, indicating resilience of circulating cells. The Integrative Biomarker Response-Threshold (IBR-T) index highlighted both hormetic and classical dose-response trends, depending on tissue type. From an ecological perspective, these findings suggest that <em>P. clarkii</em> may tolerate relatively high indomethacin concentrations without systemic oxidative collapse, potentially supporting its persistence and competitive advantage in contaminated habitats. However, the hepatopancreas sensitivity indicates possible sublethal metabolic impairment, which could affect long-term fitness and population dynamics. Overall, this study provides novel insights into the ecotoxicological effects of indomethacin in a keystone invasive species and underscore the importance of tissue-specific biomarker analysis for environmental risk assessment.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119138"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homeostatic response of calcium and potassium ions in Microcystis spp. and Chlorella to MC-LR","authors":"Chen Cheng ,&nbsp;Alan D. Steinman ,&nbsp;Qingju Xue ,&nbsp;Yilin Shu ,&nbsp;Shuyu He ,&nbsp;Yongjiu Cai ,&nbsp;Yanjie Cai ,&nbsp;Liqiang Xie","doi":"10.1016/j.ecoenv.2025.119144","DOIUrl":"10.1016/j.ecoenv.2025.119144","url":null,"abstract":"<div><div>Cyanobacteria and green microalgae often co-exist during toxic algal blooms, but the influence of microcystins (MCs) on ionic homeostasis of these algae is not well-studied. This study used non-invasive micro-test technology to examine effects of MC-LR (0, 1, 10 and 100 μg/L) on calcium (Ca²⁺) and potassium ion (K⁺) fluxes, physiological parameters related to ATP synthesis and oxidative stress, and growth, in toxic <em>Microcystis aeruginosa</em>, non-toxic <em>Microcystis wesenbergii,</em> and <em>Chlorella vulgaris</em> during a 44-day mono-culture experiment<em>.</em> Gd³⁺ treatments also were set to analyze the relationships between ion homeostasis and growth based on pharmacology experiment results. The growth of tested strains also was explored in a 28-day co-culture experiment. Results revealed that MC-LR caused the greatest percent decrease in cell density in <em>C. vulgaris</em>, with decreases ranging from −14.1 % to −31.9 % at 100 μg/L compared to the control group in the short term. In addition, there was a greater percent decrease in cell density (-2.9 % to −9.6 %) and a 10.8–24.3 % lower percent increase in cell density (depending on MC-LR concentration) in the subsequent incubation phase in <em>M. aeruginosa</em> than in <em>M. wesenbergii</em> in mono-culture experiments. In co-culture experiments, MC-LR significantly decreased the temporal stability of <em>C. vulgaris</em>, which resulted in variable population dynamics, and also caused a greater percent decrease in cell density in <em>M. aeruginosa</em> than in <em>M. wesenbergii</em> on some sampling dates. We propose that the inhibition of <em>C. vulgaris</em> stemmed from the extreme oxidative stress induced by MC-LR, whereas we attribute the inhibition of <em>M. aeruginosa</em> to a decrease in ATP content, originating from MC-LR-induced oxidative stress and decreases in ferredoxin (FDX) content and α-amylase activity. Acute and 8 days MC-LR exposure of between 1 and 100 μg/L caused a decrease in K⁺ flux in the two <em>Microcystis</em> spp. and Ca²⁺ flux in <em>C. vulgaris</em>, with a stronger decrease in K⁺ flux for <em>M. aeruginosa</em> than for <em>M. wesenbergii</em>. Pharmacology experiment results reflected the possible regulatory role of Ca²⁺ or K⁺ in MC-affected physiological indicators. These results revealed that the microbial taxa used in this study respond differently to MC-LR; the stronger self-poisoning observed in toxic <em>Microcystis</em> compared to the non-toxic strain confers a competitive advantage to the latter over other co-existing algae, thereby enhancing our understanding of the ecotoxicity and ecological function of MC-LR.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119144"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased RPE-wearing time and hand-washing frequency associated with lower urinary pollutant levels among Hong Kong e-waste workers","authors":"Gengze Liao ,&nbsp;Junye Bian ,&nbsp;Feng Wang ,&nbsp;Shi Zhao ,&nbsp;Beixi Li ,&nbsp;Yanny Hoi Kuen Yu ,&nbsp;Samuel Yeung-shan Wong ,&nbsp;Victoria H. Arrandale ,&nbsp;Alan Hoi-shou Chan ,&nbsp;Lili Liu ,&nbsp;Shaoyou Lu ,&nbsp;Lap Ah Tse","doi":"10.1016/j.ecoenv.2025.119149","DOIUrl":"10.1016/j.ecoenv.2025.119149","url":null,"abstract":"<div><div>Workers are exposed to hazardous chemicals from e-waste recycling. Protective measures such as respiratory protective equipment (RPE) and hand hygiene may reduce chemical exposure, but their effectiveness in the e-waste recycling industry remains underexplored. Therefore, a cross-sectional study was conducted from June 2021 to September 2022 involving 101 e-waste workers in Hong Kong during the COVID-19 pandemic to fill such research gap. Participants reported changes in RPE-wearing time and hand-washing frequency compared to pre-pandemic levels. First-spot morning urine samples were collected and analyzed for metals and organic pollutants. Associations between increased protective behaviors and urinary chemical concentrations were assessed using multivariate log-link linear regression models. The results showed that 31.7 % of workers increased RPE use (54.4 % of them wearing medical masks) and 49.5 % increased hand-washing frequency during the pandemic compared with pre-pandemic levels. Increased RPE use was associated with significantly lower urinary concentrations of nine chemicals. Increased hand-washing frequency showed even greater associations with diminished levels of 14 pollutants. Workers who increased both protective behaviors exhibited the greatest decreases in chemical burden. These associations were more pronounced among workers involved in dismantling/repairing e-waste and those employed by non-subsidized entities. In conclusion, enhanced preventive behaviors (RPE use and hand-washing frequency) are significantly associated with decreased chemical exposure levels among e-waste workers in Hong Kong. While engineering controls remain essential, these simple and cost-effective measures provide practical improvements in occupational safety and health, particularly for resource-limited settings where workers face elevated exposure risks due to primitive recycling practices in both developed and developing regions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119149"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of built environment on the association between nighttime heat exposure and mortality in stroke patients: A case-crossover study in Shandong Province, China","authors":"Qiyong Cao ,&nbsp;Ying Yu ,&nbsp;Siyu Sun ,&nbsp;Qiongqi Zhang ,&nbsp;Chao Liu ,&nbsp;Wanning Xia ,&nbsp;Jing Wei ,&nbsp;Chunxiang Shi ,&nbsp;Bingyin Zhang ,&nbsp;Zilong Lu ,&nbsp;Xiaolei Guo ,&nbsp;Xianjie Jia","doi":"10.1016/j.ecoenv.2025.119159","DOIUrl":"10.1016/j.ecoenv.2025.119159","url":null,"abstract":"<div><h3>Background</h3><div>Short-term exposure to extreme heat has been increasingly linked to cardiovascular mortality. However, limited evidence exists regarding the specific impact of nocturnal heat exposure on mortality among stroke patients, and the heterogeneity across residential contexts has not been fully examined.</div></div><div><h3>Methods</h3><div>Utilizing daily time-series mortality data for stroke patients in Shandong Province, China, we applied a time-stratified case-crossover design with inverse-probability weighting (IPW) to address time-varying confounding, estimating single-day and cumulative lag (0–7 days) associations between nocturnal heat exposure and mortality. Nocturnal heat was defined as the community-specific 95th percentile of mean nighttime temperature (19:00–07:00) during the warm season (May–October). Stratified analyses were conducted by age, sex, stroke subtype, and urban/rural residence. We further examined effect modification of this association by age, sex, stroke subtype, and urban/rural residence, as well as by built environment characteristics including greenness, artificial light at night (ALAN), residential land density, and building volume.</div></div><div><h3>Results</h3><div>Nocturnal heat exposure was associated with an elevated mortality risk among stroke patients, with the strongest associations at lag 1–2 days. The odds of all-cause, cardiovascular, and stroke-specific mortality increased by 17.6 % (OR 1.18, 95 % CI: 1.12–1.24), 22.1 % (OR 1.22, 95 % CI: 1.15–1.29), and 25.6 % (OR 1.26, 95 % CI: 1.17–1.35), respectively. Elevated risks were more evident among older adults (≥60 years), males, patients with hemorrhagic stroke, and rural residents. High greenness appeared to mitigate the mortality risk associated with nocturnal heat exposure, whereas other built environment factors showed limited modifying effects.</div></div><div><h3>Conclusions</h3><div>Nocturnal heat exposure was associated with short-term increases in mortality among stroke patients. Neighborhood greenness appeared to mitigate these associations, suggesting that built -environment-based adaptation strategies may help protect vulnerable populations from climate-related health risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119159"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kasugamycin and Validamycin differentially inhibit housefly larval growth through gut microbiota regulation","authors":"Xinxin Kong ,&nbsp;Shumin Wang ,&nbsp;Jinxiao Li ,&nbsp;Kexin Zhang ,&nbsp;Yansong Yin ,&nbsp;Ying Li ,&nbsp;Ruiling Zhang ,&nbsp;Zhong Zhang","doi":"10.1016/j.ecoenv.2025.119098","DOIUrl":"10.1016/j.ecoenv.2025.119098","url":null,"abstract":"<div><div>Certain bactericides exhibit dual efficacy in suppressing phytopathogens and managing insect pests. The houseflies may ingest organic matter containing bactericide residues, thereby facilitate the bioaccumulation of toxic compounds within ecosystems through trophic transfer mechanisms. Although it is known that gut microbiota regulates insect growth, immunity, and xenobiotic resistance, their responses to bactericide exposure are not well understood. This study examined the toxicological effects of two microbial-derived bactericides, Kasugamycin and Validamycin, on houseflies, along with their regulatory impacts on both the intestinal microbiota of housefly larvae and environmental microorganisms. Our research revealed that Validamycin exposure exhibited no significant effects on larval growth parameters, immune competence, or gut microbial composition. In contrast, Kasugamycin administration markedly inhibited larval development, compromised immunological defenses, and provoked histopathology modifications including fat body depletion and cuticle thinning. Microbiome analysis revealed Kasugamycin-induced dysbiosis characterized by a decrease in <em>Klebsiella</em> and <em>Enterobacter</em>, alongside an increase in <em>Pseudomona</em>s and <em>Bordetella</em>. Based on the aforementioned results, the influence of specific microbial communities' abundance shifts in the living environment on Kasugamycin toxicity was systematically assessed. The results demonstrate that <em>Enterobacter hormaechei</em> and <em>Klebsiella pneumoniae</em> enhanced larval resistance to Kasugamycin toxicity, whereas phage-mediated depletion of <em>Pseudomonas aeruginosa</em> is beneficial for the survival of housefly larvae during Kasugamycin exposure. These findings establish that the differential regulation of gut microbiota underlies the distinct effects of Kasugamycin and Validamycin on dipteran pests, providing mechanistic insights into antibiotic-insect-microbiome interactions and a theoretical framework for developing next-generation antimicrobial pest management strategies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119098"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progressive lung fibrosis in aged mice induced by repetitive bleomycin: Exacerbation by long-term PM₂.₅ inhalation","authors":"Jong-Uk Lee ,&nbsp;Yunha Nam ,&nbsp;Junyeong Baek ,&nbsp;Eunji Park ,&nbsp;Jisu Hong ,&nbsp;Ye Min Choi ,&nbsp;Seung Hyun Kim ,&nbsp;Sung Hwan Jeong ,&nbsp;Woo Jin Kim ,&nbsp;Sung Woo Park","doi":"10.1016/j.ecoenv.2025.119146","DOIUrl":"10.1016/j.ecoenv.2025.119146","url":null,"abstract":"<div><div>Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease marked by spatially and temporally heterogeneous fibrotic remodeling. Although epidemiological studies suggest that prolonged exposure to fine particulate matter (PM2.5) accelerates IPF progression, the molecular mechanisms underlying this effect remain poorly defined. The objective of this study was to establish an experimental model that better reflects the chronicity and pathological complexity of IPF. To this end, we developed a repetitive low-dose bleomycin (BLM) model in aged mice and subjected them to long-term PM2.5 inhalation. This approach induced sustained fibrosis with histological features resembling usual interstitial pneumonia (UIP), including alveolar epithelial hyperplasia, bronchiolar metaplasia, cystic remodeling, and fibroblastic foci. Transcriptomic profiling revealed extensive gene expression reprogramming, with a greater overlap with human IPF signatures than observed in the single-dose model. Notably, 27.5 % of differentially expressed genes were shared with human IPF lungs, particularly those involved in extracellular matrix remodeling, epithelial repair, and immune modulation. PM2.5 exposure further exacerbated fibrotic remodeling, reduced survival, and increased fibrotic marker expression. Additional transcriptomic analysis identified PM2.5-specific gene signatures enriched in mitotic dysregulation, chromatin remodeling, and epithelial stress responses, with key hub genes such as BNIP3, SERPINE1, and BIRC5. In conclusion, our repetitive BLM model in aged mice effectively recapitulates the histopathological and molecular landscape of IPF and provides a physiologically relevant platform for studying chronic fibrogenesis. This model also enables mechanistic investigation of environmental exacerbators such as PM2.5, offering new insights into gene–environment interactions that drive pollutant-induced fibrotic progression.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119146"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental microplastics contamination and its impact on thyroid function in newborns","authors":"Xuan Zhang ,&nbsp;Li Li ,&nbsp;Yue Zhang ,&nbsp;Bingying Liu ,&nbsp;Xiaoyan Wang ,&nbsp;Lei Sun","doi":"10.1016/j.ecoenv.2025.119056","DOIUrl":"10.1016/j.ecoenv.2025.119056","url":null,"abstract":"<div><div>Microplastics (MPs), which have been identified in human tissues such as the placenta, may pose potential risks to newborn health by interfering with thyroid function, a critical regulator of growth and development. This study investigates the association between placental microplastic levels and thyroid hormone profiles in newborns. We investigated the effect of placental microplastic exposure on fetal thyroid hormone levels in a cohort of 1250 mother-child pairs enrolled between 2022 and 2024. Umbilical cord blood thyroid hormone concentrations (free thyroxine [T4], free triiodothyronine [T3], and thyroid-stimulating hormone [TSH]) were measured, and placental microplastics (PVC, PP, and PBS) were quantified. Multivariable regression models (MLR), quantile g-computation (g-comp) and Bayesian Kernel Machine Regression (BKMR) were used to assess the effects of microplastics on thyroid hormone levels, adjusted for relevant covariates. Adjusted MLR models confirmed that individual microplastic types, including PVC, PP, and PBS, were significantly associated with reductions in T4 levels (β = –1.40, –0.74, and –0.85, respectively) and TSH levels (β = –1.27, –2.87, and –1.94, respectively), as well as decreases in the T4/T3 ratio (β = –5.60, and –2.68 for PVC and PP, respectively). PP exposure was the only microplastic type associated with an increase in T3 levels (β = 0.05; 95 % CI: 0.01, 0.10; p = 0.03). G-comp analysis showed that each quartile increases in exposure corresponded to significant decreases in T4 (β = –1.78; 95 % CI: –2.38, –1.18), TSH (β = –3.72; 95 % CI: –4.71, –2.72), and the T4/T3 ratio (β = –6.24; 95 % CI: –8.53, –3.94; p &lt; 0.01 for all). BKMR analysis further revealed a dose–response relationship, with higher microplastic exposure linked to decreasing T4 levels and a lower T4/T3 ratio, while T3 and TSH exhibited only minimal changes across exposure quantiles. This study reveals that placental microplastic exposure is linked to reductions in newborn thyroid hormone levels, particularly T4 and the T4/T3 ratio, with dose-response effects observed across exposure quantiles.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119056"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of environmental fluoroquinolones on the motor system in zebrafish: Reversible?","authors":"Jiali Bao ,&nbsp;Xinxin Zeng ,&nbsp;Xin Cong ,&nbsp;Kan Yu ,&nbsp;Yushu Qiu ,&nbsp;Xuben Yu ,&nbsp;Liya Zhang ,&nbsp;Yuhang Wu ,&nbsp;Weixi Zhang ,&nbsp;Lisu Huang","doi":"10.1016/j.ecoenv.2025.119116","DOIUrl":"10.1016/j.ecoenv.2025.119116","url":null,"abstract":"<div><div>Fluoroquinolones (FQs) are prevalent in aquatic ecosystems, posing potential environmental risks. While clinical doses of FQs are known to cause severe musculoskeletal and neurological effects in children, the severity, duration, and reversibility of their effects on motor function remain unclear. In response to these concerns, this study investigated the impact of four widely encountered FQs—ciprofloxacin, norfloxacin, ofloxacin, and levofloxacin—on zebrafish. Firstly, to evaluate the severity and duration of FQ exposure effects, we assessed locomotor activity and histopathology. Zebrafish embryos were exposed to environmental levels of FQs. Results showed significant reductions in swimming distance and activity by half in FQ-exposed zebrafish at 14 days post-fertilization (dpf). Additionally, increased myofiber size and cartilage ratios were observed in norfloxacin- and ofloxacin-exposed adult zebrafish (120 dpf). Furthermore, biomarker analysis revealed decreased creatine kinase and myoglobin contents, as well as increased Sox9 expression, indicating disrupted cartilage development. To further explore the severity and reversibility of FQ effects, we examined acute high-dose levofloxacin exposure in juvenile zebrafish (28 dpf). Significant decreases were observed in the total distance, single muscle fiber area, cartilage proportion, and content of Sox9. Locomotor activity and cartilage morphology were assessed across different recovery periods, with markers returning to normal 14 days after exposure. Overall, our findings suggest that both chronic low-dose and acute high-dose FQ exposure led to motor system toxicity in zebrafish, highlighting potential ecological risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119116"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triclosan exposure exacerbates muscle wasting via senescence and IGF-1 pathway suppression: A combined epidemiological and experimental study","authors":"Zhenlin Yang ,&nbsp;Liangshi Hao ,&nbsp;Amila Kuati ,&nbsp;Xiang Wang ,&nbsp;Ziling Qian ,&nbsp;Changning Hao ,&nbsp;Junli Duan ,&nbsp;Boshen Yang ,&nbsp;Yuankang Zhu","doi":"10.1016/j.ecoenv.2025.119142","DOIUrl":"10.1016/j.ecoenv.2025.119142","url":null,"abstract":"<div><div>Triclosan, a broad-spectrum bactericide in various consumer products, has become a ubiquitous environmental contaminant, with detectable residues found in aquatic systems and human urine. Although studies demonstrated its potential to impair muscle strength, population-level evidence and mechanistic insights linking triclosan exposure to muscle health remain underexplored. This study leveraged 2878 participants data from NHANES, and suggested that individuals in the highest quartile of urinary creatinine-corrected triclosan levels exhibited a 0.18 kg/m² reduction in appendicular skeletal muscle mass index (ASMI) [β = −0.18, 95 % CI (−0.35, −0.01)] compared to those in the lowest quartile. Importantly, bilirubin was identified as an oxidative stress mediator, explaining 16.799 % of this adverse association. Experimental evidence determined that triclosan accelerates muscle wasting through oxidative imbalance and muscle senescence. Insulin-like growth factor 1 (IGF-1) served as an essential role in the development of triclosan-induced muscle wasting. Molecular docking and molecular dynamics simulations revealed that triclosan could directly interact with IGF-1. The results of experiments demonstrated that triclosan induces muscle senescence and oxidative stress by inhibiting the IGF-1/Akt/mTOR signaling pathway. Furthermore, administration of IGF-1 protein mitigated muscle wasting by reducing oxidative stress and muscle senescence, offering a potential mechanism for counteracting triclosan-induced muscle wasting. This study not only underscores the detrimental effects of triclosan on muscle health but also establishes a mechanistic link between triclosan exposure and muscle wasting. These findings emphasize the need for environmental and public health policies to alleviate the negative effects of triclosan on muscle health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119142"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrolytic manganese residue amendment improved the suitability of lead-zinc tailings for plant growth","authors":"Mengting Lin ,&nbsp;Xusheng Jiang ,&nbsp;Xuehong Zhang ,&nbsp;Luyang Wang ,&nbsp;Hui Qiu ,&nbsp;Huachun Lan ,&nbsp;Guo Yu ,&nbsp;Jie Liu","doi":"10.1016/j.ecoenv.2025.119158","DOIUrl":"10.1016/j.ecoenv.2025.119158","url":null,"abstract":"<div><div>Revegetation is urgently needed for the ecological restoration of lead-zinc tailings ponds. However, the harsh properties of lead-zinc tailings—including poor aggregation, nutrient deficiency, and low water retention capacity—severely hinder plant colonization and growth. This study effectively improved the physical structure and nutrient status of lead-zinc tailings using electrolytic manganese residue (EMR), a solid waste characterized by strong cohesiveness and high ammonium nitrogen content. Moreover, lead-zinc tailings amended with 1 % EMR had low leaching of toxic elements, indicating low environmental risk. X-ray computed tomography and nuclear magnetic resonance analyses revealed that EMR amendment significantly reduced total porosity of lead-zinc tailings while enhancing their water retention capacity (<em>p</em> &lt; 0.05). The improvements likely resulted from the filling of interparticle voids by EMR clay particles and the formation of Ca₂(AlFe)(Si₂O₇)(SiO₄)O(OH), a hydrated gel similar to the hydrated calcium silicate gel. The hydrated gel formed by the reaction between Ca²⁺ from EMR and silicates on tailing surfaces may increase interparticle cohesion. A pot experiment showed that 1 % EMR amendment significantly promoted ryegrass growth in tailings, increasing fresh biomass by 13.7 % (<em>p</em> &lt; 0.05). Overall, these findings indicate that EMR amendment is a promising strategy to enhance plant colonization and growth in the revegetation of lead-zinc tailings ponds.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119158"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}