Ecotoxicology and Environmental Safety最新文献

{"title":"Agricultural soil microbiomes at the climate frontier: Nutrient-mediated adaptation strategies for sustainable farming","authors":"Jianfeng Du ,&nbsp;Qixiong Gao ,&nbsp;Fuxin Sun ,&nbsp;Baoyou Liu ,&nbsp;Yang Jiao ,&nbsp;Qili Liu","doi":"10.1016/j.ecoenv.2025.118161","DOIUrl":"10.1016/j.ecoenv.2025.118161","url":null,"abstract":"<div><div>The equilibrium transformation of soil microbial community dynamics and succession across various temporal and spatial dimensions plays a critical role in maintaining plant adaptability. Intensive agricultural practices accelerate the succession of plant microbial communities, rendering their restoration function more vulnerable. Climate change, with its variable impacts, affects the resilience of plant microbial communities through regulatory and mediating effects. Investigating the spatiotemporal dynamics of soil microbial communities in the context of climate change offers valuable insights into developing robust and resilient microbial ecosystems. This review examines the regulatory role of soil resources in plant microbial communities, the interactive effects of climate change on soil resource regulation, and the prediction of microbial community structures through resource allocation. Additionally, it explores the mechanisms that sustain ecological resilience in plant microbial community systems, emphasizing the application of the profit-averaging law.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118161"},"PeriodicalIF":6.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of metals with hemoglobin and anemia in a Chinese early adolescent cohort","authors":"Mengyuan Yuan ,&nbsp;Ting Tang ,&nbsp;Yonghan Li ,&nbsp;Xinhui Wang ,&nbsp;Cong Wang ,&nbsp;Xueying Zhang ,&nbsp;Yichao Huang ,&nbsp;Fangbiao Tao ,&nbsp;Gengfu Wang ,&nbsp;Daomin Zhu ,&nbsp;Feng Geng ,&nbsp;Puyu Su","doi":"10.1016/j.ecoenv.2025.118122","DOIUrl":"10.1016/j.ecoenv.2025.118122","url":null,"abstract":"<div><h3>Background</h3><div>The relationship between exposure to metals with hemoglobin levels and anemia in children and adolescents has shown inconsistency. This study aimed to evaluate the associations of individual metals and metal mixtures with hemoglobin levels and anemia risk, as well as potential sex differences, in a Chinese early adolescent cohort.</div></div><div><h3>Methods</h3><div>Data was obtained from a Chinese early adolescent cohort with two-year follow-up periods. Serum concentrations of 12 metals were measured using inductively coupled plasma mass spectrometry (ICP-MS). To examine the individual associations of metals with hemoglobin levels and anemia risk, a series of single-metal and multi-metal adjusted generalized linear mixed models (GLMMs) were applied. Additionally, quantile g-computation (qgcomp) and Bayesian kernel machine regression (BKMR) were used to analyze the effects of metal mixture. Furthermore, all analyses were stratified by sex.</div></div><div><h3>Results</h3><div>The multi-metal adjusted GLMMs identified significant associations of As (β=2.31; 95 % CI: 0.15, 4.47), Cd (β=-2.11; 95 % CI: −4.11, −0.11), Cr (β=-10.19; 95 % CI: −19.09, −1.30), Cu (β=-7.27; 95 % CI: −14.27, 0.26), Fe (β=13.71; 95 % CI: 10.03, 17.38), Pb (β=7.87; 95 % CI: 4.21, 11.5), V(β=-13.60; 95 % CI: −21.32, −5.88), and Zn (β=14.77; 95 % CI: 4.38, 25.15) with hemoglobin concentration, as well as As (OR=0.26; 95 % CI: 0.11, 0.60), Co(OR=4.27; 95 % CI: 1.25, 14.6), Cr (OR=10.49; 95 % CI: 1.61, 68.39), Fe (OR=0.03; 95 % CI: 0.01, 0.12) and Pb (OR=0.18; 95 % CI: 0.04, 0.80) with anemia risk. Moreover, the qgcomp revealed no association of metal mixture with hemoglobin concentration (β=0.94; 95 % CI=-0.45, 2.33) or anemia risk (OR=0.81; 95 % CI: 0.51, 1.27). After stratification by sex, the qgcomp demonstrated no significant overall effect of the metal mixture on hemoglobin concentration or anemia risk in either boys (β=0.93; 95 % CI: −0.84, 2.71 for hemoglobin, and OR=0.64; 95 % CI: 0.27, 1.52 for anemia) or girls (β=0.93, 95 % CI: −1.16, 3.01 for hemoglobin, and OR=0.72; 95 % CI: 0.40, 1.32 for anemia). Similarly, sex-stratified BKMR models also revealed no significant association between the metal mixture and hemoglobin concentration or anemia risk in either boys or girls.</div></div><div><h3>Conclusions</h3><div>This study highlights the individual and collective impacts of 12 metals on hemoglobin and anemia during early adolescence, underscoring the need for experimental and larger cohort studies to further corroborate these findings.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118122"},"PeriodicalIF":6.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-safe potential of FITC-tagged nFeO in enhancing alfalfa-rhizobia symbiosis and salt stress tolerance via physicochemical and ultrastructural modifications","authors":"Hafiz Abdul Kareem ,&nbsp;Yongdong Li ,&nbsp;Sana Saleem ,&nbsp;Adnan Mustafa ,&nbsp;Muhammad Azeem ,&nbsp;Quanzhen Wang ,&nbsp;Song Li ,&nbsp;Yi Chen ,&nbsp;Xihui Shen","doi":"10.1016/j.ecoenv.2025.118158","DOIUrl":"10.1016/j.ecoenv.2025.118158","url":null,"abstract":"<div><div>Salt stress severely limits global crop productivity by disrupting ionic balance, physiological processes, and cellular ultrastructure, particularly in salt-sensitive forages like alfalfa (<em>Medicago sativa</em> L). Addressing this issue requires environmentally feasible and innovative strategies. This study investigated the comparative potential of Nano-FeO and FeSO₄ (30 mg kg⁻¹) soil supplements with rhizobium on alfalfa salt tolerance employing morphological, physicochemical, and cellular approaches. The results demonstrated that FITC-nFeO and rhizobium significantly reduced Na⁺ uptake, enhanced K⁺ accumulation, and improved the Na⁺/K⁺ ratio in alfalfa roots and shoots relative to FeSO₄. Scanning electron microscopy illustrated that FITC-nFeO ameliorated root ultracellular structure and leaf stomatal functionality, facilitating improved gaseous exchange characteristics and photosynthetic performance. Confocal laser scanning microscopy confirmed FITC-tagged nFeO adhesion to roots, supported by transmission electron microscopy findings of preserved chloroplast ultrastructure under FITC-nFeO and rhizobium application. FITC-nFeO also mitigated oxidative damage of ROS, as evidenced by reduced hydrogen peroxide, electrolyte leakage, and thiobarbituric acid reactive substances (TBARS) content, through enhanced antioxidant enzyme activities. Overall, in comparison to FeSO₄, FITC-nFeO with rhizobium retrieved the salt-induced damages in alfalfa by promoting morpho-physiological and ultracellular integrity. This study highlights the role of nanotechnology in enhancing the resilience of forages on salt-contaminated soils, paving the way for eco-friendly remediation strategies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118158"},"PeriodicalIF":6.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mediating mechanism of Oct4 in the process of cleft palate induced by 2, 3, 7, 8-tetrachlorodibenz-p-dioxin (TCDD) and all-trans retinoic acid","authors":"Zengli Yu ,&nbsp;Yaxin Zhang ,&nbsp;Shiyi Wu ,&nbsp;Ziyan Sun ,&nbsp;Zhan Gao ,&nbsp;Yao Chen ,&nbsp;Xiangdong Wang ,&nbsp;Dongliang Xu ,&nbsp;Xiaozhuan Liu","doi":"10.1016/j.ecoenv.2025.118102","DOIUrl":"10.1016/j.ecoenv.2025.118102","url":null,"abstract":"<div><div>2, 3, 7, 8-tetrachlorodiphenyl-p-dioxin (TCDD) and all-trans retinoic acid (atRA) have been shown to be inducers of cleft palate (CP). Relevant studies have shown that Oct transcription factor 4 (Oct4) can regulate the expression of the aromatic hydrocarbon receptor (AhR), a target gene of TCDD, as well as influence the retinoic acid signaling pathway. However, the mechanism of Oct4 regulating the interaction between TCDD and atRA in CP pathogenesis remains unclear. In order to solve the above problems, mouse embryonic palatal mesenchymal (MEPM) cells were used for further study. The results show that, TCDD and atRA induce a similar pattern of CP in vivo and significantly inhibit the proliferation of MEPM cells. In vitro, TCDD exposure led to up-regulation of AhR and cytochrome P450 1A1 (CYP1A1) expression and down-regulation of cellular retinoic acid binding protein 2 (CRABP2) and retinoic acid receptor-alpha (RARA) expression in MEPM cells. In addition, atRA exposure resulted in decreased expression of AhR and CYP1A1, and increased protein levels of RARA and CRABP2. When Oct4 was overexpressed, the down-regulation of CRABP2 and RARA by TCDD and the down-regulation of AhR by RA were reversed. The cross results of mass spectrometry and Biogrid database show that HSP90AB1 can bind to Oct4 and AhR. Co-immunoprecipitation (Co-IP) results showed that Oct4 and CRABP2 were combined, which further suggested the mediating effects of Oct4 in CP development. In summary, we hypothesize that AhR-HSP90AB1-Oct4-CRABP2 axis may play the key role mediating the interaction between TCDD and atRA in CP pathogenesis.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual indicator for the detection of methylmercury in blood: A critical biomarker for dietary exposure assessment","authors":"Yan Guo ,&nbsp;Bing-chan Ma ,&nbsp;Wen-qi Zhang,&nbsp;Bo-xin Li,&nbsp;Jia-ming Ou,&nbsp;Fen Liu,&nbsp;Chang-ye Hui","doi":"10.1016/j.ecoenv.2025.118157","DOIUrl":"10.1016/j.ecoenv.2025.118157","url":null,"abstract":"<div><div>Methylmercury (MeHg), a significant dietary exposure biomarker, poses a substantial threat to public health, primarily due to the consumption of aquatic foods. Current detection methods are complex and unsuitable for on-site testing, necessitating the development of a simple and sensitive biosensor for rapid screening. This study addresses this challenge by developing a highly sensitive whole-cell biosensor for detecting organic mercury in blood samples, offering both visual qualitative and colorimetric quantitative assessments. We engineered a biosensor based on the <em>mer</em> operon and deoxyviolacein (DV) pigment, optimizing its performance by adjusting the MerB expression level, screening host cells, and incorporating the biosurfactant rhamnolipid. The optimized biosensor achieved a detection limit of 0.195 nM and exhibited a linear response range of 0.195–1.563 nM for MeHg. This range is significantly below the Provisional Tolerable Weekly Intake (PTWI) of 1.6 μg/kg body weight per week established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA), ensuring the reliable detection of MeHg at concentrations well within the safety threshold. Notably, the biosensor demonstrated broad-spectrum detection capabilities, including natural MeHg and synthetic organomercurials, which are crucial for assessing dietary exposure risks from various sources. This study advances the development of a novel biosensor for MeHg detection, highlighting its potential as a critical tool for assessing dietary exposure risks and contributing to understanding food safety and public health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118157"},"PeriodicalIF":6.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential threat of environmental toxin palytoxin to cerebral nerves: A mechanism study in vitro and in vivo","authors":"Xue Zhang ,&nbsp;Lei Huang ,&nbsp;Yinliang Liu ,&nbsp;Xuewen Li ,&nbsp;Huanying Zhou ,&nbsp;Kang Qin ,&nbsp;Shuang Li ,&nbsp;Shuyue Ren ,&nbsp;Xuexia Jia ,&nbsp;Zhixian Gao","doi":"10.1016/j.ecoenv.2025.118150","DOIUrl":"10.1016/j.ecoenv.2025.118150","url":null,"abstract":"<div><div>Palytoxin (PTX), a toxin naturally synthesized by marine organisms like <em>Palythoa</em>, <em>Ostreopsis</em> and <em>Trichodesmium</em> spp. in tropical and temperate seas, bioaccumulates in fish and crustaceans, thereby exposing humans through the food chain. Although growing evidence highlights PTX's lethal hepatotoxicity, nephrotoxicity, and cardiotoxicity, its neurotoxic effects and the underlying mechanisms remain elusive. In this study, we assessed the cerebral neurotoxicity of PTX by using HT22 neuronal cells and a chronic mouse model, conducting a comprehensive analysis of phenotypic alterations and gene expression changes. Phenotypic analysis revealed significant damage to mitochondria, endoplasmic reticulum, and axons and disruptions in energy metabolism in PTX-treated neuronal cells and mouse brains. Transcriptome sequencing and real-time quantitative reverse transcription polymerase chain reaction indicated that key genes in the JNK/p38 MAPK signaling, mitochondrial stress, and endoplasmic reticulum stress pathways were significantly altered. Furthermore, pretreatment with JNK and p38 inhibitors significantly restored mitochondrial membrane potential, ATP content, and cell viability, while reducing the expression of pro-apoptotic genes in HT22 cells. These findings confirm that JNK/p38 MAPK signaling pathways activation, leading to mitochondrial stress, is a major contributor to PTX-induced neuronal cell death at the cellular level. Chronic exposure to PTX was shown to damage mammalian cerebral nerves, carrying a potential risk for neurodegenerative diseases. Our study provides insights into the environmental and health risks associated with PTX exposure and offers a foundation for risk assessment and intervention strategies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118150"},"PeriodicalIF":6.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the link between Di-2-ethylhexyl phthalate (DEHP) exposure and muscle mass: A systematic investigation utilizing NHANES data analysis, network toxicology and molecular docking approaches","authors":"Jia-qi Hao ,&nbsp;Biao Ran ,&nbsp;Shu-yue Hu ,&nbsp;Zi-xuan Zhuang ,&nbsp;Jia-wan Zhang ,&nbsp;Meng-rui Xiong ,&nbsp;Rui Wang ,&nbsp;Wen Zhuang ,&nbsp;Mo-jin Wang","doi":"10.1016/j.ecoenv.2025.118132","DOIUrl":"10.1016/j.ecoenv.2025.118132","url":null,"abstract":"<div><div>Sarcopenia is a syndrome characterized by a progressive, widespread decline in muscle mass and strength. DEHP, a plasticizer involved in daily life and widely used, has been found in various everyday items and causes developmental dysregulation, reproductive impairments, tumorigenesis, and transgenerational disease. However, much remains to be discovered regarding the association between exposure to this environmental toxin and sarcopenia, as well as the toxic targets and molecular mechanisms. This research elucidated the relationship between contact with DEHP and the development of sarcopenia by integrating NHANES data analysis, network toxicology, and molecular docking. 3199 adults were enrolled, and multiple linear regressions were performed to reveal a significant negative correlation between lnDEHP and ALM_BMI. Eighty-eight targets associated with DEHP and sarcopenia were identified. Subsequent STRING and Cytoscape screening stressed 20 key targets, including CASP3, BCL2, MMP9, BCL2L1, APP, and CTSS. GO and KEGG enrichment analyses revealed that these targets are involved in ligand-receptor interactions, apoptosis, and calcium signaling pathways. Molecular docking simulations using CB-dock confirmed the high-affinity binding interactions between DEHP and these key targets. This study validated the relationship between DEHP exposure and muscle mass. Further, it provided a theoretical basis for investigating the molecular mechanisms of DEHP exposure-induced skeletal muscle toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)","authors":"Jingchong Li ,&nbsp;Wende Zheng ,&nbsp;Jingkun Li ,&nbsp;Komelle Askari ,&nbsp;Zhixiang Tian ,&nbsp;Aohui Han ,&nbsp;Runqiang Liu","doi":"10.1016/j.ecoenv.2025.118146","DOIUrl":"10.1016/j.ecoenv.2025.118146","url":null,"abstract":"<div><div>Chlorpyrifos (CHP) contamination affects agricultural land and poses significant risks to plants and humans. Chitosan-oligosaccharide (COS) enhances plant resilience under stress and boosts the activity of enzymes metabolizing exogenous substances. This study aimed to explore the potential and mechanism of COS in mitigating CHP phytotoxicity and reducing CHP accumulation through both pot and field experiments. The results indicated that CHP exposure caused oxidative stress and decreased photosynthesis by 18.5 % in wheat. COS up-regulated the expression of antioxidant enzyme genes in CHP-stressed plants, resulting in a 12.1 %-29.4 % increase in antioxidant enzyme activity, which resulted in an 11.3 %-12.8 % reduction in reactive oxygen species (ROS) and an 11.5 %-14.7 % reduction in malondialdehyde (MDA) content in leaves and roots, respectively. Additionally, COS increased chlorophyll content by 6.6 % by regulating genes related to chlorophyll metabolism, enhancing photosynthesis by 13.6 %. COS also reduced CHP uptake and accelerated its metabolism by upregulating <em>CYP450</em>, <em>GST</em>, and lignin biosynthesis-related genes. Wheat treated with COS exhibited a 26.7 %-28.7 % reduction in grains' CHP content, resulting in a lower health risk index (HRI). These findings provide novel insights into the potential of COS in alleviating CHP phytotoxicity and reducing its accumulation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics insights into antioxidant and immune responses in Penaeus monodon under ammonia-N, low salinity, and combined stress","authors":"Yundong Li ,&nbsp;Sheng Huang ,&nbsp;Song Jiang ,&nbsp;Lishi Yang ,&nbsp;Jianhua Huang ,&nbsp;Qibin Yang ,&nbsp;Ziyi Jiang ,&nbsp;Jianzhi Shi ,&nbsp;Zhenhua Ma ,&nbsp;Erchao Li ,&nbsp;Falin Zhou","doi":"10.1016/j.ecoenv.2025.118156","DOIUrl":"10.1016/j.ecoenv.2025.118156","url":null,"abstract":"<div><div>Ammonia nitrogen and salinity are critical environmental factors that significantly impact marine organisms and present substantial threats to <em>Penaeus monodon</em> species within aquaculture systems. This study utilized a comprehensive multi-omics approach, encompassing transcriptomics, metabolomics, and gut microbiome analysis, to systematically examine the biological responses of shrimp subjected to low salinity, ammonia nitrogen stress, and their combined conditions. Metabolomic analysis demonstrated that exposure to ammonia nitrogen stress markedly influenced the concentrations of antioxidant-related metabolites, such as glutathione, suggesting that shrimp mitigate oxidative stress by augmenting their antioxidant capacity. The transcriptomic analysis revealed an upregulation of genes linked to energy metabolism and immune responses and antioxidant enzymes. Concurrently, gut microbiome analysis demonstrated that ammonia nitrogen stress resulted in a marked increase in <em>Vibrio</em> populations and a significant decrease in <em>Photobacterium</em>, indicating that alterations in microbial community structure are intricately associated with the shrimp stress response. A comprehensive analysis further indicated that the combined stressors of ammonia nitrogen and salinity exert a synergistic effect on the immune function and physiological homeostasis of shrimp by modulating antioxidant metabolic pathways and gut microbial communities. These findings provide critical systematic data for elucidating the mechanisms through which ammonia nitrogen and salinity influence marine ecosystems, offering substantial implications for environmental protection and ecological management.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118156"},"PeriodicalIF":6.2,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycium barbarum polysaccharide protects BMSCs against cadmium-induced suppression of osteogenic differentiation by modulating autophagy","authors":"Liang-xu Wei ,&nbsp;Yan Cui ,&nbsp;Yan-lan Lv ,&nbsp;Qian-yun Ye ,&nbsp;Jian-bing Chen ,&nbsp;Xiao-yao Zhan ,&nbsp;Zi-jun Zhu ,&nbsp;Xiao-feng Zhu ,&nbsp;Bei Zhou","doi":"10.1016/j.ecoenv.2025.118148","DOIUrl":"10.1016/j.ecoenv.2025.118148","url":null,"abstract":"<div><div>Cadmium (Cd), a heavy metal pollutant that accumulates in organisms, is osteotoxic and contributing to the development of osteoporosis. Lycium barbarum polysaccharide (LBP), a polysaccharide obtained from the Chinese medicinal plant Lycium barbarum, exhibits protective effects against oxidative stress and cellular damage. However, it is unclear whether LBP can effectively inhibit Cd-induced osteotoxicity. This study investigated whether LBP alleviates Cd-induced osteogenic suppression in bone marrow mesenchymal stem cells (BMSCs) via autophagy modulation. Cell viability, osteogenic markers (ALP, BMP2, COL1, RUNX2), and mineralization were assessed using CCK-8, qPCR, Western blot, and alizarin red staining. Autophagy flux was evaluated via GFP-mCherry-LC3B transfection and protein markers (LC3-II, P62, BECN). We confirmed that Cd inhibited the formation of cellular autophagosomes while adversely affecting BMSCs. However, LBP mitigated the Cd-induced osteogenic inhibitory effect by stimulating the autophagy processes and facilitating the formation of autophagosomes and autolysosomes. In conclusion, LBP is a potential bone-enhancing agent that can improve cell viability and osteogenic differentiation while mitigating the detrimental effects of Cd on BMSCs through the activation of autophagy.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"295 ","pages":"Article 118148"},"PeriodicalIF":6.2,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}