Ecotoxicology and Environmental Safety最新文献

{"title":"Assessment of microplastic toxicity on blood-testis barrier using 3D cell spheroids","authors":"Yucheng Liu ,&nbsp;Chunyuan Du ,&nbsp;Hanjing Li ,&nbsp;Liqun Wang ,&nbsp;Yuan Zhu ,&nbsp;Fanrong Ai ,&nbsp;Kui Zhou","doi":"10.1016/j.ecoenv.2025.119062","DOIUrl":"10.1016/j.ecoenv.2025.119062","url":null,"abstract":"<div><div>Industrial production generates large quantities of microplastics (MPs), which can significantly impact ecosystems and pose potential risks to human health, both directly and indirectly, and the mechanisms and potential toxicity of microplastics affecting the reproductive system remain unclear. In this study, we investigated the cytotoxicity of microplastics on the blood-testis barrier using 3D Sertoli cell spheroids (TM4S) exposed to artificially manufactured primary polystyrene microplastics (MPS). To investigate these effects, we explored the invasiveness of MPS in relation to microplastic size and concentration, and analyzed morphological changes in the cell spheroids. In addition, we evaluated changes in cellular physiological activities. Finally, we detected pathways related to endoplasmic reticulum stress in Sertoli cells (TM4), and revealed that exposure to MPS triggered endoplasmic reticulum stress in TM4S. This model has the potential to deepen our understanding on the microplastic-reproduction interaction and the underlying toxicity mechanisms of microplastics in the reproductive system.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119062"},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Study on the mechanism of penetration of particles of air pollution into the brain through the nasal cavity and the potential neurological health risks\" [Ecotoxicol. Environ. Saf. 303 (2025) 118963].","authors":"Yi Zhang, Haitong Zhang, Haiyan Li, Jialing Li, Hongmei Wei, Yifan Wang, Tieshan Wang, Shouying Du, Pengyue Li","doi":"10.1016/j.ecoenv.2025.119076","DOIUrl":"10.1016/j.ecoenv.2025.119076","url":null,"abstract":"","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":" ","pages":"119076"},"PeriodicalIF":6.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural compound library screening identifies triptolide for the treatment of okadaic acid-induced intestinal dysfunction","authors":"Ji-qian-zhu Zhang ,&nbsp;Xiao-yu Dai ,&nbsp;Jing-jing Mao ,&nbsp;Lang Yan ,&nbsp;Li-jun Ren ,&nbsp;Jin-feng Li ,&nbsp;Yi-jun Tian ,&nbsp;Wen-lin Li ,&nbsp;Jiang-bo Zhu ,&nbsp;Ji-kuai Chen","doi":"10.1016/j.ecoenv.2025.119042","DOIUrl":"10.1016/j.ecoenv.2025.119042","url":null,"abstract":"<div><div>Okadaic acid (OA), a diarrhetic shellfish toxin, causes significant intestinal and systemic toxicity. Currently, there are no approved therapeutics specifically targeting OA-induced diarrhea. Using high-throughput screening of 2131 natural compounds, we identified triptolide as a compound conferring protection against OA-induced apoptosis. In mice exposed to OA, triptolide administration reduced intestinal permeability and ameliorated mucosal injury. In HUVECs, triptolide (0.1–3 μM) dose-dependently increased cell viability and suppressed apoptotic activity. Biotin-based labeling identified the hydroxyl group as the essential active site of triptolide. RNA sequencing analysis showed that triptolide inhibited the OA-induced upregulation of endoplasmic reticulum (ER) protein synthesis-related genes. Furthermore, triptolide prevented the OA-induced decrease in the p-eIF-2α/eIF-2α ratio, thereby attenuating ER stress. These results demonstrate that triptolide mitigates OA-induced toxicity by inhibiting ER stress-mediated endothelial cell apoptosis and maintaining intestinal barrier integrity, highlighting its potential as a therapeutic agent against OA-related poisoning.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119042"},"PeriodicalIF":6.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of methodological conditions and model interpretation in the adsorption of a nanomaterial on a volcanic soil","authors":"Manuel Gacitua ,&nbsp;Angelo Neira-Albornoz","doi":"10.1016/j.ecoenv.2025.119075","DOIUrl":"10.1016/j.ecoenv.2025.119075","url":null,"abstract":"<div><div>Soil adsorption governs the environmental fate of nanomaterials, yet standard procedures often fail to fully capture adsorption behaviour through kinetic and isotherm models. This study proposes a comprehensive framework to enhance the reliability and interpretability of adsorption data by improving both experimental design and model analysis, using the adsorption of Cu₂O nanoparticles from the commercial pesticide NORDOX 75 WG (NOR) in Metrenco soil (Ultisol). Batch adsorption kinetics and isotherm experiments were conducted. NOR stability and supernatant pH were the most influential factors and were used to select a subset of consistent, comparable data. Transport-related parameters (e.g., kinetic rates) were more sensitive to NOR stability than bulk-adsorbent parameters (e.g., adsorption capacity). We applied a novel validation strategy combining traditional goodness-of-fit metrics (including adjusted <span><math><msup><mrow><mi>R</mi></mrow><mrow><mn>2</mn></mrow></msup></math></span>) with intra-model assumption checking, and inter-model comparison. While the linear Pseudo-Second Order model (PSO) achieved the highest <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>adj</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span>, a combined analysis of multiple nonlinear models provided stronger evidence for non-equilibrium condition and apparent instantaneous adsorption, in contrast to the interpretation derived from linear PSO parameters. This approach also led to the formulation of a modified PSO model equation, better aligned with the system behaviour and consistent with trends observed in other models. Unlike typical studies that rely on a single “best-fitting” model, our method enables deeper mechanistic insights, even from poorly fitting models. This addresses a widespread but overlooked issue in the literature and supports more robust modelling of complex adsorption systems to support environmental decision-making.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119075"},"PeriodicalIF":6.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of commercial insecticides on survival of the red mason bee Osmia bicornis","authors":"Anna Misiewicz ,&nbsp;Maryellen Zbrozek ,&nbsp;Ryszard Laskowski ,&nbsp;Agnieszka J. Bednarska","doi":"10.1016/j.ecoenv.2025.119023","DOIUrl":"10.1016/j.ecoenv.2025.119023","url":null,"abstract":"<div><div>Insecticides are widely used to control pests, but can also be toxic to ecosystem service providers, including bees. Moreover, their efficacy can increase if they are applied in mixtures. These mixtures can be intentional, e.g., two or more active substances formulated in one product or created by farmers as tank mixtures (i.e., a mixture of different, individually formulated plant protection products (PPP) applied together in one application event or applied at short intervals), or unintentional, when exposure to a mixture results from bees foraging on different crops, each of which is treated with a different PPP. Although studies on the combined toxicity of various PPPs are increasing, the impact of mixture is still not adequately addressed in environmental risk assessment, posing a potential threat to bees. Here, we assessed the interactive effects of five insecticides belonging to different chemical classes: organophosphate Dursban 480 EC (a.s. chlorpyrifos), two pyrethroids – Sherpa 100 EC (a.s. cypermethrin) and Karate Zeon 050 CS (a.s. lambda-cyhalothrin), neonicotinoid Mospilan 20 SP (a.s. acetamiprid), and sulfoximine Closer (a.s. sulfoxaflor)) applied topically alone and as binary mixtures, on survival of <em>Osmia bicornis</em> females. Contrary to expectations, the experiment revealed either no interaction (in ‘Dursban × Sherpa’ experiment) or antagonistic interactions, particularly in mixtures of insecticide belonging to pyrethroids (Sherpa or Karate) with the one belonging to either neonicotinoids (Mospilan) or sulfoximines (Closer). Moreover, the mixture ‘Karate × Closer’ suggests a potential antagonistic effect on the survival of <em>O. bicornis</em> already at field-relevant concentrations. Such unexpected results emphasize the need for longer-term testing of cumulative toxicity effects via different exposure routes and on different bee species, as well as the need to consider the risk of exposure to multiple pesticides when assessing the safety of pesticides for bees.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119023"},"PeriodicalIF":6.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between acrylamide and glycidamide hemoglobin adduct levels and chronic kidney disease: A NHANES analysis","authors":"Wen-Tao Xu ,&nbsp;Wen-Cai Zheng ,&nbsp;Chao-Ran Chen ,&nbsp;Fei Lin ,&nbsp;Shao-Hao Chen ,&nbsp;Xiao-Dong Li ,&nbsp;Xiong-Lin Sun ,&nbsp;Qing-Shui Zheng ,&nbsp;Yong Wei ,&nbsp;Xue-Yi Xue ,&nbsp;Zhi-Bin Ke ,&nbsp;Ning Xu","doi":"10.1016/j.ecoenv.2025.119072","DOIUrl":"10.1016/j.ecoenv.2025.119072","url":null,"abstract":"<div><h3>Background</h3><div>Acrylamide (AA) is a dietary contaminant with experimental nephrotoxicity, yet its relationship with chronic kidney disease (CKD) remains unclear.</div></div><div><h3>Methods</h3><div>Utilizing NHANES data (2003–2006, 2013–2016), we analyzed 10,431 participants to examine associations between hemoglobin-bound AA (HbAA), glycidamide (HbGA), and CKD prevalence. Associations were evaluated through weighted multivariate logistic regression and dose-response analysis with restricted cubic splines. Additionally, toxicological profiling, target identification, and pathway enrichment analysis were performed.</div></div><div><h3>Results</h3><div>Higher HbAA, HbGA, and combined biomarkers were non-linearly associated, with lower CKD prevalence at higher quartiles and evidence of threshold effects on RCS. A significant negative association was observed for HbAA (highest vs. lowest quartile OR = 0.78, 95 % CI: 0.59–1.01, <em>P</em>-trend = 0.033) and HbGA (OR = 0.74, 95 % CI: 0.54–1.01, <em>P</em>-trend = 0.041). Dose-response analysis indicated a non-linear relationship, suggesting threshold effects potentially explained by hormesis, younger demographics, or confounding dietary and lifestyle factors. Computational modeling identified potential key molecular targets (TP53, AKT1, BCL2, JUN, MAPK3, TNF) that may mediate AA's biological effects.</div></div><div><h3>Conclusion</h3><div>The inverse relationship between AA exposure biomarkers and CKD identified in this large population-based study challenges current toxicological paradigms. This may be due to hormesis, demographic confounding, or methodological limitations. Longitudinal studies are needed to validate these paradoxical results.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119072"},"PeriodicalIF":6.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a Pseudomonas strain mitigates the combined stresses of phenolic acids and salt in soil-grown cucumber.","authors":"Jisheng Zhao, Hongrui Bai, Xiu-Juan Wang, Wenhao Zhang, Pi-Yao Sun, Ruijie Ren, Xinhui Zhang, Caixia Xia, Ning Lyu, Xuan Liu, Ji-Gang Bai","doi":"10.1016/j.ecoenv.2025.118822","DOIUrl":"10.1016/j.ecoenv.2025.118822","url":null,"abstract":"<p><p>As both phenolic acids and salt accumulate in greenhouse soil, soil-grown plants in greenhouses are exposed to both phenolic acid and salt stresses. However, no method for mitigating these combined stresses has been reported for soil-grown plants. In this study, we examined whether bacterial inoculation could alleviate the combined stresses of phenolic acids and salt and facilitate plant productivity in greenhouse. A Pseudomonas strain referred to as CFA was separately inoculated into medium and cucumber-planted soil supplemented with ferulic acid (FA), p-hydroxybenzoic acid (PHBA), and a salt (sodium chloride, sodium sulfate, sodium carbonate, or sodium bicarbonate). CFA showed plant growth-promoting properties and could degrade FA and PHBA in salt-supplemented medium. After application of CFA into FA-, PHBA-, and salt-supplemented soil, CFA colonization was observed, and the levels of malonaldehyde and hydrogen peroxide in the leaves were found to be decreased. Meanwhile, the activities of glutathione peroxidase, ascorbate peroxidase, superoxide dismutase, and catalase in cucumber and those of phosphatase, urease, sucrase, and catalase in soil were increased. Moreover, CFA application to soil improved cucumber growth, reduced FA and PHBA concentrations in soil, enhanced soil polysaccharide contents, and altered the rhizospheric bacterial communities. We thus propose that CFA application affects rhizospheric bacterial communities; it degrades FA and PHBA in soil thus decreasing their concentrations, induces antioxidant enzymes in cucumber, increases the activities of soil enzymes, and enhances soil polysaccharide contents, thus mitigating the combined stresses of FA, PHBA, and salt.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"303 ","pages":"118822"},"PeriodicalIF":6.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambient temperature, heat exposure and diminished ovarian reserve among women undergoing assisted reproductive technologies.","authors":"Xinyu Ji, Baode Xue, Ningxin Zhang, Shuangyan Liu, Lin Liu, Mingjian Fu, Xin Ye, Wei Wang, Mengxiao Yang, Zulipiye Aisan, Lingfei Cui, Xinliu Zeng, Kuai Yu","doi":"10.1016/j.ecoenv.2025.118870","DOIUrl":"10.1016/j.ecoenv.2025.118870","url":null,"abstract":"<p><p>Association between heat exposure and diminished ovarian reserve (DOR) among women undergoing assisted reproductive technologies remains underexplored. We conducted a retrospective cohort study of 4296 women from a reproductive medicine center in Wuhan, China. Ambient temperature exposures were estimated based on the women's residential address and then averaged over short-term (1 month, 3 months, and 6 months) and long-term (1 year, 2 years, and 3 years) exposure windows. Heatwave exposure was defined using absolute (≥ 35°C) and relative (90th/95th percentiles) temperature thresholds over consecutive days (D2: >2 days; D3: >3 days), and was further categorized into high exposure (above median), low exposure (median and below) and no exposure. Multivariable logistic regression models were used to estimate odds ratios (ORs) for DOR (Anti-Müllerian hormone [AMH] < 1.1 ng/mL). Each standard deviation (SD) increase in mean, maximum, and apparent temperature over a 1-month period was associated with a 37 % (2 %-83 %), 36 % (2 %-80 %), and 55 % (4 %-131 %) higher odds of DOR. Compared to no exposure, 1-year heatwave exposure (35°C-D3) was associated with a 64 % (5 %-156 %) higher odds of DOR. Low-frequency heatwave exposure over 1 year (90th-D3: OR=1.33 [1.02-1.74]) and 3 years (95th-D2: OR=1.41 [1.08-1.70]) also showed significant associations, while high-frequency heat exposure did not exhibit such effects. Similarly, low-duration heatwave exposure over the 3-year period was associated with higher DOR risk. Higher temperatures and heatwaves exposure were associated with higher risk of DOR. Highlighting the needs to both short-term temperature variability and prolonged low-frequency heatwaves, rather than focusing solely on extreme high-intensity events.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"303 ","pages":"118870"},"PeriodicalIF":6.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of wheat straw-derived amendments combined with sulfate increases soil microbial diversity, reduces soil cadmium mobility and cadmium accumulation in rice.","authors":"Xianlin Ke, Songyan Li, Guoxi Wang, Xiaosong Yang, Mingming Tao, Yanhua Qiu, Yifei Li, Yu Liu, Lijuan Huang, Zhengyi Hu","doi":"10.1016/j.ecoenv.2025.118916","DOIUrl":"10.1016/j.ecoenv.2025.118916","url":null,"abstract":"<p><p>Cadmium (Cd) accumulation in rice grains poses severe health risks. Conventional straw amendments exhibit inconsistent efficacy, likely because of variations in amendment types and soil sulfur deficiency. The pot experiment was evaluated in a Cd-contaminated paddy soil (3.18 mg Cd kg^-1) to compare three treatments (1.0 % w/w) sources: WS (wheat straw), CW (wheat straw compost), and BW (wheat straw biochar) without or with sodium sulfate fertilization (30 mg sulfur kg^-1). This experiment aimed to evaluate the Cd mobility, microbial diversity, and Cd accumulation in rice tissues. All treatments resulted in reduced Cd accumulation in brown rice at the maturity stage by 3 % (WS), 26 % (CW), and 60 % (BW), whereas sulfate co-application enhanced this reduction to 30 % (WS+S), 38 % (CW+S), and 66 % (BW+S). This could be attributed to decrease in soil Cd availability (17 %, 37 %, and 41 % for WS+S, CW+S, and BW+S, respectively, at the maturity stage), inhibition of Cd uptake by root iron plaque (-0.01 to -0.05), and decrease of Cd translocation in rice tissues (particularly phloem transfer). Microbial analysis revealed that biochar and compost preferentially increased bacterial alpha diversity and shifted microbial composition irrespective of sulfate supply, especially at the tillering and booting stages. Specifically, biochar and compost reduced Firmicutes (positively related to exchangeable Cd, P ≤ 0.01), and increased Bacteroidota and Proteobacteria (negatively related to exchangeable Cd, P ≤ 0.01) at these stages. These findings demonstrate that co-application of wheat straw biochar and sulfate offers a solution for remediating Cd-contaminated paddy soils while considering soil ecosystem health.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"303 ","pages":"118916"},"PeriodicalIF":6.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of polyethylene microplastics on carbendazim degradation by Rhodococcus sp. XY-1: Molecular mechanisms and soil bioremediation effects.","authors":"Zi-Yue Ding, Zi-Wang Yuan, Zi-Wei Hua, Xiao-Yue Hu, Chun-Yue Chai, Lin Zhang, Zhi-Wen Xi, Tie-Jun Wang, Qiu-Hong Niu, Hao Zhang","doi":"10.1016/j.ecoenv.2025.118917","DOIUrl":"10.1016/j.ecoenv.2025.118917","url":null,"abstract":"<p><p>Carbendazim persists in soil, causing harm to the environment. Microbial degradation is a main way to remove carbendazim from soil, and polyethylene (PE), as a kind of microplastics, widely exists in soil. However, the mechanism by which PE influences carbendazim biodegradation is still unclear. This study isolated Rhodococcus sp. XY-1, a highly efficient carbendazim-degrading bacterium capable of completely degrading 50 mg·L^-1 carbendazim, as its sole carbon and nitrogen source within 4 d. Scanning electron microscopy revealed that PE (700 µm, 5 %) depressed XY-1 cell surfaces but induced protective intercellular substance adhesion. According to cellular reactive oxygen species (ROS) assays and Fourier transform infrared spectroscopy, PE exposure increased intracellular ROS levels in XY-1 and enhanced the absorption intensity of characteristic protein and nucleic acid peaks, indicating metabolic stimulation. Consequently, PE accelerated carbendazim degradation by strain XY-1, achieving a 79.0 % removal of 50 mg·L^-1 carbendazim within 60 h. Furthermore, PE itself underwent oxidation and hydrolysis, as evidenced by the simulated soil experiments at an increased carbon-to-oxygen ratio (from 0.016 to 0.072) and the emergence of -OH functional groups. PE also enhanced soil microbial activity, regulated organic carbon content, and influenced carbendazim adsorption, collectively promoting its degradation. Under PE (700 µm, 5 %) amendment, strain XY-1 achieved a degradation rate of 58.0 % for 5.0 mg·kg^-1 carbendazim within 7 d in soil. This study elucidates the mechanism by which microplastics influence the microbial degradation of soil organic pollutants.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"303 ","pages":"118917"},"PeriodicalIF":6.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}