Ecotoxicology and Environmental Safety最新文献

{"title":"Metagenome-metabolome responses to linarin alleviate hepatic inflammatory response, oxidative damage, and apoptosis in an ETEC-challenged weaned piglet model","authors":"Chaofan Sun ,&nbsp;Xiaodan Liu ,&nbsp;Mingyang Wang ,&nbsp;Qianqian Zhang ,&nbsp;Hanwen Geng ,&nbsp;Xu Ji ,&nbsp;Hongyu Wang ,&nbsp;Shenghe Li ,&nbsp;Erhui Jin ,&nbsp;Feng Zhang","doi":"10.1016/j.ecoenv.2025.119145","DOIUrl":"10.1016/j.ecoenv.2025.119145","url":null,"abstract":"<div><div>Enterotoxigenic <em>Escherichia coli</em> (ETEC), present in contaminated food, water, and environments, can induce hepatic injury via the gut-liver axis, posing a serious threat to ecological systems and public health. Linarin, a flavonoid extracted from <em>Chrysanthemum indicum</em>, exhibits anti-inflammatory and antioxidant properties, but its protective effects against ETEC-induced hepatic injury remain unclear. In this study, 24 weaned piglets were randomly assigned to four groups: BD+NB (basal diet + nutrient broth), LN+NB (basal diet + 150 mg/kg linarin + nutrient broth), BD+ETEC (basal diet + ETEC challenge), and LN+ETEC (basal diet + 150 mg/kg linarin + ETEC challenge). Dietary linarin significantly increased ADFI and the genes related to oxidative damage and bile acid metabolism, while decreasing F:G ratio, liver index, serum liver function-related parameters, and the genes related to inflammatory response and apoptosis. It also significantly altered the relative abundances of gut microbiota, which were closely associated with key hepatic metabolic pathways, including nicotinate and nicotinamide metabolism and fatty acid biosynthesis. Our study suggests that linarin alleviated ETEC-induced hepatic inflammation and apoptosis, enhanced antioxidant capacity, and regulated bile acid metabolism. The potential mechanism involves linarin modulating gut microbiota-mediated key hepatic metabolic pathways to exert protective effects. In contrast to previous flavonoid-ETEC studies that primarily focused on the gut, this study, based on the gut-liver axis, investigates the potential mechanisms by which linarin is associated with the alleviation of ETEC-induced hepatic injury through integrated analysis of gut microbiome metagenomics and liver metabolomics.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119145"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PARP1 promoter hypermethylation promotes arsenic-induced skin damage by driving telomere dysfunction-mediated keratinocyte senescence","authors":"Luming Yang,&nbsp;Wenjuan Wang,&nbsp;Aihua Zhang","doi":"10.1016/j.ecoenv.2025.119108","DOIUrl":"10.1016/j.ecoenv.2025.119108","url":null,"abstract":"<div><div>Arsenic, a common environmental pollutant, causes skin damage with long-term exposure. Although its pathogenic mechanism remains unclear, skin cell senescence creates a microenvironment that promotes cancer development, with the mechanisms accelerating disease progression in arsenic-induced skin damage attracting significant attention. This study utilised previously collected skin samples to evaluate the associations between skin senescence and damage indicators. According to the presence or absence of arsenic exposure, participants were divided into a reference group and an arsenic exposure group. Additionally, the arsenic exposure group was further divided into a common pathological changes group, a hyperkeratosis group, and a skin cancer group, based on skin histopathological examination. Compared with the reference group, the arsenic exposure group exhibited increased expression of senescence-associated secretory phenotypes (IL-6 and IL-17) and shortened relative telomere length (RTL). With increasing severity of skin damage, IL-6 and IL-17 levels progressively increased, while RTL progressively decreased. Examination of representative indicators of arsenic-induced skin damage (epithelial-mesenchymal transition [EMT] indicators) revealed decreased E-cadherin expression and increased vimentin expression. With increasing severity of skin damage, E-cadherin expression progressively decreased, while vimentin expression progressively increased. Moreover, clear correlations were observed between senescence-related markers (IL-6, IL-17, and RTL) and arsenic-induced skin damage markers (E-cadherin, vimentin) in the human samples. <em>In vitro</em> experiments demonstrated that arsenic induced lower expression of the telomere-related gene PARP1, reducing its binding to TERF2 and weakening its recruitment of BLM, thereby causing telomere dysfunction, promoting the senescence of HaCaT cells, and resulting in EMT. Additionally, arsenic exposure induced high expression of DNMT3, which mediated PARP1 hypermethylation and low expression. Treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine restored PARP1 expression in arsenic-treated HaCaT cells, regulated telomere dysfunction, improved cellular senescence, and alleviated EMT. This study provides new insights into the mechanisms underlying arsenic-induced skin damage from an epigenetic and genetic perspective.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119108"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Florfenicol-induced gut microbiome dysbiosis triggers chronic disease pathways and intestinal damage in the benthic fish (Leiocassis longirostris)","authors":"Yang Feng,&nbsp;Senyue Liu,&nbsp;Chengyan Mou,&nbsp;Zhipeng Huang,&nbsp;Zhongmeng Zhao,&nbsp;Han Zhao,&nbsp;Jian Zhou,&nbsp;Qiang Li,&nbsp;Yongqiang Deng","doi":"10.1016/j.ecoenv.2025.119139","DOIUrl":"10.1016/j.ecoenv.2025.119139","url":null,"abstract":"<div><div>The sub-therapeutic use of antibiotic use in aquaculture disrupts host-microbiota balance and poses ecological risks, though its chronic effects on benthic species remain poorly understood. Here we demonstrate that exposing Chinese longsnout catfish (<em>L. longirostris</em>) to florfenicol at a standard aquaculture dose of 10 mg/kg per day for five days induced severe intestinal dysbiosis and functional impairment. We observed a significant 43.65 % reduction in microbial gene abundance and a 96.16 % decrease in host mRNA expression, accompanied by a marked 69.11 % increase in host DNA contamination, indicating the collapse of the mucosal barrier and loss of microbial ecological function. The core microbiota shifted from beneficial <em>Cetobacterium</em>, which produces antioxidant and neuroprotective metabolites, to opportunistic genera such as <em>Clostridium</em> and <em>Acinetobacter</em>. Metabolically, florfenicol exposure resulted in a significant 29.3 % suppression of functional pathways and a pronounced 5.8-fold upregulation of electron transport genes including <em>petB</em> and <em>fprB</em>, which is associated with the activation of chronic diseases pathways such as Parkinson’s, Huntington’s and Diabetic cardiomyopathy. These pathways all share common mechanisms with mitochondrial dysfunction and ROS metabolism and further trigger chronic pathological changes including intestinal mucosal injury, mitochondrial oxidative stress and cell apoptosis. Additionally, the loss of <em>Cetobacterium</em> impaired the synthesis of key metabolites such as ascorbic acid and lovastatin acid, exacerbating intestinal structural damage and apoptosis. The transfer risk of antibiotic resistance genes was also significantly elevated. These findings reveal persistent microbiome dysfunction and heightened chronic disease risks following conventional antibiotic exposure, underscoring the urgency of reevaluating antibiotic usage in sustainable aquaculture.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119139"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo analyses of embryotoxicity and teratogenicity of gold nanoparticles: Emphasis on the surface chemistry and toxicological responses","authors":"Tayaba Ismail ,&nbsp;Yong-Gyu Jeong ,&nbsp;Hyun-Kyung Lee ,&nbsp;Hongchan Lee ,&nbsp;Youni Kim ,&nbsp;Jun-Yeong Lee ,&nbsp;Sang-Hyun Kim ,&nbsp;Hong-Yeoul Ryu ,&nbsp;Taeg Kyu Kwon ,&nbsp;Tae Joo Park ,&nbsp;Taejoon Kwon ,&nbsp;Dongwoo Khang ,&nbsp;Hyun-Shik Lee","doi":"10.1016/j.ecoenv.2025.119111","DOIUrl":"10.1016/j.ecoenv.2025.119111","url":null,"abstract":"<div><div>The surface chemistry and physical characteristics of gold nanoparticles (AuNPs) influence their biological interactions and toxicological responses. However, the toxicological effects of surface charge on embryonic development remain poorly understood. In this study, we investigated the <em>in vivo</em> developmental toxicity and teratogenicity of differentially charged AuNPs during early embryogenesis of <em>Xenopus laevis</em> – a sensitive and ecologically relevant animal model for developmental toxicology. Our study indicated that cationic AuNPs induced significant embryotoxicity and teratogenicity including lethality, phenotypical abnormalities and disruption of gene expression associated with liver, digestive tract, neural, and eye development. In contrast, such effects, including lethality and malformations associated with changes in gene expression were not observed in embryos exposed to anionic AuNPs. In addition, cationic AuNPs affected ciliogenesis by reducing the number of multiciliated cells and disturbing cilia-driven fluid flow, a critical endpoint in nanoparticle-induced toxicity. Furthermore, gene expression profiles suggested that necroptosis might be the mechanism of cell death in embryos exposed to cationic AuNPs. Notably, the surface charge dependent AuNPs exposure leading to impaired ciliogenesis and activation of necroptosis during embryogenesis represents significant endpoints in nanotoxicology. Unlike previous studies focusing on zebrafish or rodents, this study provides the first systematic evaluation in <em>X. laevis</em> embryos with identical nanoparticle cores but distinct surface chemistries. Our study underscores the significance of nanoparticle surface functionalization in determining developmental toxicity and pinpoints the ecological risks imposed by cationic AuNPs during early embryonic development in aquatic systems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119111"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis and risk assessment of antibiotic contaminants and the antibiotic resistance of Escherichia coli from crested ibis","authors":"Jing Yang,&nbsp;Xueyan Wang,&nbsp;Yun Wang,&nbsp;Zekun Xu,&nbsp;Juanli Yun,&nbsp;Junkang Guo","doi":"10.1016/j.ecoenv.2025.119126","DOIUrl":"10.1016/j.ecoenv.2025.119126","url":null,"abstract":"<div><div>The environmental quality of habitat is a critical determinant of animal survival. In this study, we conducted a comprehensive analysis of ten antibiotic residues in habitats of the crested ibis (<em>Nipponia nippon</em>). Results indicated that tetracyclines (TCs) were the most predominant species, constituting over 89 % of the total antibiotics detected. A comprehensive risk assessment revealed that oxytetracycline (OTC) and sulfamethoxazole (SMZ) posed medium and high ecological risk, respectively, based on risk quotients (RQ) analysis. Higher antibiotic concentrations and ecological risks were observed in the Hanzhong region compared to other areas. Furthermore, antimicrobial susceptibility testing of <em>Escherichia coli</em> isolates from crested ibis showed complete resistance to TC and chlortetracycline (CTC), as well as high resistance rates to ciprofloxacin (CIP), norfloxacin (NOR) and enrofloxacin (ENR). The antibiotic resistance genes <em>tetA</em>, <em>gryB</em>, <em>bla</em>_<em>TEM</em> and <em>bla</em>_<em>SHV</em> were highly prevalent among the isolates. Notably, <em>E. coli</em> isolates from Hanzhong region exhibited higher resistance rate and a greater variety of antibiotic resistance genes. These findings could enhance our understanding of antibiotic contamination and the associated spread of antimicrobial resistance of <em>E. coli</em> within crested ibis habitats, providing a scientific basis for improved antibiotic risk management in these ecosystems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119126"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of dimethylformamide at different developmental stages of Biomphalaria glabrata: Lethal concentration determination and longitudinal assessment of physiological changes","authors":"Jiyuan Wang ,&nbsp;Ruiqi Weng ,&nbsp;Yunhuan Zhu ,&nbsp;Jialu Xu ,&nbsp;Xianwei Li ,&nbsp;Qingzhi Zhao ,&nbsp;Yijie Zhang ,&nbsp;Xinyi Fei ,&nbsp;Ruke Wang ,&nbsp;Xiaodong Yao ,&nbsp;Xiaofen Zhang ,&nbsp;Jiangyue Zhong ,&nbsp;Keda Chen ,&nbsp;Lijun Lin ,&nbsp;Hongyu Li","doi":"10.1016/j.ecoenv.2025.119131","DOIUrl":"10.1016/j.ecoenv.2025.119131","url":null,"abstract":"<div><div>Dimethylformamide (DMF) is a widely used polar organic solvent with potential hepatotoxicity and environmental release risks. DMF, as an important industrial solvent, poses a high risk of environmental leakage, particularly in aquatic environments, where it may have toxic effects on aquatic organisms, particularly gastropods. Gastropods are an important component of aquatic ecosystems, yet no studies have focused on the toxic effects of DMF on this group. In this study, the freshwater gastropod <em>Biomphalaria glabrata</em> was employed as a model organism to evaluate both the acute and chronic toxicity of DMF at embryonic, newly hatched, and adult stages. We hypothesized that DMF would exert stage-specific, concentration-dependent toxic effects on multiple biological functions, including survival, growth, reproduction, immune responses, and tissue structure. Static exposure experiments revealed LC₅₀ values of 0.46 % (216 h), 0.58 % (96 h), and 1.67 % (96 h) for embryos, newly hatched juveniles, and adults, respectively. Chronic exposure over 21 days showed that DMF significantly inhibited shell growth, body weight gain, and oviposition in a concentration-dependent manner. Hematological assessments demonstrated that hemolymph volume, cell density and viability declined progressively with increasing DMF concentrations, suggesting immunosuppressive effects. Histopathological analysis further revealed progressive structural damage in both the hepatopancreas and gonadal tissues. This study provides a comprehensive characterization of DMF toxicity across different developmental stages of <em>B. glabrata</em>, demonstrating its adverse effects on survival, growth, reproduction, immune function, and tissue structure. The results extend current understanding of polar organic solvent toxicity in aquatic invertebrates and offer a solid experimental basis for further research in environmental and immunotoxicology.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119131"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment and migration of pathogenic microorganisms and antibiotic resistance genes during a severe haze-snowfall pollution in northern China","authors":"Rongbao Duan,&nbsp;Xueting Yang,&nbsp;Qun He,&nbsp;Houfeng Liu,&nbsp;Pengju Xu,&nbsp;Min Wei","doi":"10.1016/j.ecoenv.2025.119130","DOIUrl":"10.1016/j.ecoenv.2025.119130","url":null,"abstract":"<div><div>Atmosphere serves as a significant reservoir and habitat for pathogenic bacteria and antibiotic resistance genes (ARGs). Wet deposition facilitates their entry into terrestrial ecosystems, posing potential health risks. This study investigated the harmful biological components during a winter haze-snowfall event in a northern Chinese city. The findings revealed an elevated PM_2.5 concentration of 309 µg/m³ during severe pollution, which was associated with high concentrations of water-soluble ions and microorganisms. The composition of microbial communities varied in response to the intensification of pollution leading up to snowfall. Prior to snowfall, the dominant phylum was Proteobacteria (83.2 %), with predominant genera including <em>Sphingomonas</em> (16.3 %), <em>Bradyrhizobium</em> (13.6 %), <em>Phyllobacterium</em> (10.6 %), <em>Caulobacter</em> (9.7 %) and <em>Afipia</em> (7.8 %). Following snowfall, the dominant phylum shifted to Bacteroidetes (38.9 %), with key genera being <em>Prevotella</em> (28.3 %), <em>Aliiarcobacter</em> (11.7 %), <em>Staphylococcus</em> (10.7 %), and <em>Pseudomonas</em> (6.9 %). Harmful bioaerosol components were enriched in snow samples, including pathogenic bacteria such as <em>Afipia broomeae</em>, <em>Tsukamurella tyrosinosolvens</em>, <em>Escherichia coli</em>, <em>Aliiarcobacter cryaerophilus</em>, <em>Staphylococcus aureus</em>, and <em>Parabacteroides distasonis</em> along with ARGs like <em>macB</em>, <em>tetA (58)</em>, <em>evgS</em>, <em>adeL</em>; these accounted for up to 75 % of pathogenic bacteria present in snow water. A notable enrichment of metabolic pathways related to human diseases following snowfall events observed within clean samples post-snowfall; this increase may be attributed to enrichments occurring during haze pollution that were subsequently transported from the upper atmosphere as analyzed through air mass backward trajectories. This research provides valuable insights into the distribution patterns of pathogenic microorganisms and ARGs within particulate matter and snow samples, meanwhile elucidating preliminary understandings regarding the enrichment and migration mechanisms of high-risk bioaerosol components facilitated by wet deposition during periods of severe air pollution.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119130"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory effects and underlying mechanisms of glomalin-related soil protein on the environmental behavior of heavy metals","authors":"Anqi Li ,&nbsp;Qingliang Cui ,&nbsp;Sheng Zhan ,&nbsp;Shaoyuan Zhang ,&nbsp;Kai Ning ,&nbsp;Qi Li ,&nbsp;Hansong Chen","doi":"10.1016/j.ecoenv.2025.119147","DOIUrl":"10.1016/j.ecoenv.2025.119147","url":null,"abstract":"<div><div>Glomalin-related soil protein (GRSP), a widely distributed glycoprotein complex in soil, has a high potential for heavy metal pollution remediation due to its unique physicochemical properties and synergistic effects with plant roots. However, lack of comprehensive studies on regulatory mechanisms of GRSP in heavy metal pollution restricts its application in soil pollution risk management. This review synthesizes current knowledge on GRSP biosynthetic pathways, structural characteristics, and of heavy metal transport regulation in the soil-plant system using a multidisciplinary analysis of molecular biology, ecology, and environmental science. As a key biogeochemical regulator at the soil-plant interface, GRSP influences heavy metal speciation through various mechanisms. Its surface functional groups (e.g., carboxyl and hydroxyl) form stable coordination complexes with heavy metal ions, enhance soil particle aggregation, and restrain heavy metal bioavailability by modifying the physicochemical and biochemical properties of the rhizosphere soil. Collectively, these functions mitigate heavy metal phytotoxicity and enhance plant metabolic resilience. This review establishes a theoretical framework for sustainable environmental pollution management and ecological restoration by systematically elucidating GRSP sequestration capacity, bioavailability control, and environmental responsiveness, providing new insights and theoretical support for the development and application of green and efficient heavy metal-contaminated soil remediation technologies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119147"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lambda-cyhalothrin exposure enhances the development of atherosclerosis in ApoE−/− mice","authors":"Lei Yu ,&nbsp;Haiyan Chen ,&nbsp;Lingcheng Li ,&nbsp;Haofeng Zhang ,&nbsp;Fudong He ,&nbsp;Zhenger Fang ,&nbsp;Guangjun Zheng ,&nbsp;Biying Zhou ,&nbsp;Xia Chen ,&nbsp;Mingliang Liu ,&nbsp;Xiaohua Liang ,&nbsp;Guolong Qi ,&nbsp;Zhoubin Zhang ,&nbsp;Guang Hao","doi":"10.1016/j.ecoenv.2025.119165","DOIUrl":"10.1016/j.ecoenv.2025.119165","url":null,"abstract":"<div><h3>Background</h3><div>Lambda-cyhalothrin (LCT), a widely used pyrethroid insecticide, has been implicated in the development of metabolic disorders. However, its specific role in atherosclerosis (AS) remains unclear.</div></div><div><h3>Objectives</h3><div>This study aimed to investigate the influence of LCT on AS development in ApoE^−/−mice and to explore the potential role of metabolomic alterations.</div></div><div><h3>Methods</h3><div>Eight-week-old male ApoE^−/− mice were fed a high-fat diet for 12 weeks to establish an AS model. Fifteen AS model mice were randomly allocated into three groups (n = 5 per group) for a 15-day intervention: (1) a normal control group (NC), (2) a 45 % high-fat diet (HFD), and (3) an HFD with lambda-cyhalothrin exposure group (HFD+LCT, 1 mg/kg/day). After the intervention, fecal, serum, and aortic samples were collected. Atherosclerotic lesions were evaluated using Oil Red O staining of aortic tissues, and serum lipid profiles were measured. Fecal metabolomic profiling was performed using liquid chromatography-mass spectrometry (LC-MS). Additionally, bioinformatics analysis of two independent gene expression datasets (GSE122165 and GSE124081) was conducted to elucidate the molecular pathways associated with pyrethroid exposure.</div></div><div><h3>Results</h3><div>Compared with the NC and HFD groups, the HFD+LCT group exhibited significantly increased aortic plaque formation and elevated serum triglyceride (TG) levels. Untargeted metabolomic analysis revealed substantial perturbations in the HFD+LCT group compared to the HFD group, with 159 significantly altered metabolites (108 increased and 51 decreased). Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis identified nine pathways potentially involved in LCT-aggravated AS, including lipid and AS, cholesterol metabolism, and glycerolipid metabolism. The triglyceride species TG (16:0/16:1/16:1) was identified as a potential mediator. Transcriptome analysis suggested that LCT may influence AS risk by regulating the expression of genes such as <em>Cyp3a13</em>, <em>Cyp2b10</em>, <em>Vnn1</em>, <em>Hsd3b5</em>, <em>Hsd3b2</em>, and <em>Gapdhs</em>, thereby affecting metabolic processes.</div></div><div><h3>Conclusion</h3><div>LCT exacerbates atherosclerotic lesions in ApoE^−/−mice, and fecal metabolomic alterations may contribute to this process.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119165"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The competitive adsorption of phenanthrene and benzo[a]pyrene by fractionated soil particles in individual and coexistence pollution","authors":"Mingyue Qi ,&nbsp;Guohui Ning ,&nbsp;Xiaomin Wang ,&nbsp;Meng Zhang ,&nbsp;Meng Liu ,&nbsp;Jiahui Li ,&nbsp;Jiayuan Gao ,&nbsp;Guangwei Zhang ,&nbsp;Rui Hao ,&nbsp;Xuefeng Zhao ,&nbsp;Zhixin Yang","doi":"10.1016/j.ecoenv.2025.119073","DOIUrl":"10.1016/j.ecoenv.2025.119073","url":null,"abstract":"<div><div>Two polycyclic aromatic hydrocarbons (PAHs), benzo[<em>a</em>]pyrene (BaP) and phenanthrene (Phe), were added alone and in combination to unfractionated and fractionated soil (fine sand, silt, clay) in batch equilibrium experiments to determine their adsorption. The maximum adsorption capacity increased with initial concentrations for both PAHs, with a stronger increase for BaP. The BaP adsorption capacity was promoted by coexistence of Phe, but Phe adsorption capacity was inhibited by coexistence of BaP. Their adsorption isotherms during coexistence best fitted the Freundlich equation, giving a lesser fit with a linear equation that improved by coexistence. The <em>n</em> of the Phe isotherm in coexistence with BaP wasclose to 1 for the fine sand and silt. The adsorption of Phe and BaP was strongest in clay, followed by fine sand and then silt. Single Phe adsorption in clay was 23.82∼61.33 % higher than in fine sand and silt, and for BaP it was 5.78∼8.35 % higher. In silt, the Phe adsorption efficiency was reduced by 39.73 % in coexistence with BaP, while the BaP adsorption efficiency improved with 19.69 % in coexistence. Coexistence triggered a mechanistic redistribution: it weakened Phe partitioning, while it strengthened BaP surface adsorption. The adsorption behavior of these PAHs in coexistence for different soil fractions provides insights for optimal remediation strategies of PAH-contaminated soils.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119073"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}