Ecotoxicology and Environmental Safety最新文献

{"title":"Integrated analysis of transcriptome and metabolome revealed clomazone biodegradation in maize seedlings","authors":"Guofeng Chen ,&nbsp;Jipeng Xu ,&nbsp;Jing Wang ,&nbsp;Wengong Huang ,&nbsp;Feng Liu ,&nbsp;Jiannan Dong ,&nbsp;Baohai Liu ,&nbsp;Dongmei Shi ,&nbsp;Aihua Cheng ,&nbsp;Hui Liao","doi":"10.1016/j.ecoenv.2025.118302","DOIUrl":"10.1016/j.ecoenv.2025.118302","url":null,"abstract":"<div><div>Clomazone (CMZ) is a pesticide widely used for weed control in soybean fields. However, its persistence in the environment, including soil, surface water, and groundwater, poses potential risks to subsequent crops and human health. To evaluate the ecotoxicological impacts of CMZ residues on maize growth, a comprehensive study was conducted using integrated transcriptomic and metabolomic analyses of maize seedlings. The results showed that maize seedlings absorb CMZ through the roots and translocate it to the shoots, which led to inhibited growth, reduced chlorophyll content, decreased dry weight, increased electrolyte leakage, and elevated antioxidant enzyme activities. Differentially expressed metabolites (DEMs) and genes (DEGs) were significantly altered in CMZ-stressed maize seedlings, with 1456 DEGs and 1461 DEMs in roots, and 2946 DEGs and 2999 DEMs in shoots. Metabolomic profiling revealed the accumulation of key metabolites involved in CMZ catabolism, including carbohydrates, amino acids, glutathione, and flavonoids. UPLC-Q-TOF/MS analysis identified twelve CMZ transformation products (TPs), which correlated with the activities of DEGs, DEMs, and antioxidant enzymes. These findings indicate that maize seedlings detoxify absorbed CMZ through specific pathways, including decarboxylation, and primarily via canonical phase I and phase II reactions. This study suggests that crops like maize can mitigate the toxicity and residues of CMZ, providing insights for strategies to manage and control CMZ ecotoxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118302"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crucial role of the Pht1;4 Gene in Sb(V) tolerance and uptake in Arabidopsis thaliana","authors":"Ziyi Dong ,&nbsp;Mengchang He ,&nbsp;Chunye Lin ,&nbsp;Wei Ouyang ,&nbsp;Xitao Liu","doi":"10.1016/j.ecoenv.2025.118308","DOIUrl":"10.1016/j.ecoenv.2025.118308","url":null,"abstract":"<div><div>There has been increasing awareness of the risks of antimony (Sb) in the environment, but the process of Sb(V) absorption by plants and its effects on plants remain unclear. This study focused on four independent T-DNA insertion mutant strains of <em>Arabidopsis thaliana</em> and wild-type (WT) plants to investigate their tolerance, uptake, and response to Sb(V). Compared with those of the WT, the Pht1;4 knockout mutant M-P4 presented greater tolerance to Sb(V) and lower absorption levels. The roots of the M-P4 were longer and the malondialdehyde (MDA) content in the roots of M-P4 was lower than WT (0.194 &lt; 1.333, μM/mg FW). The amount of Sb(V) absorbed by the roots of M-P4 under Sb(V) treatment was lower than that absorbed by WT plants (by 25 %-50 %), and the levels of Sb in the stems and leaves were also lower. Moreover, the transmembrane transport ratio of Sb(V) in M-P4 was lower than that in the WT (0.748 &lt; 0.937). The Pht1;1 knockout mutant exhibited a predominant transmembrane absorption mode for Sb(V), while gene expression data show that knocking out either Pht1;1 or Pht1;4 leads to the upregulation of the other gene. These results collectively demonstrate that the characteristics of M-P4 are due to the important role of Pht1;4 in Sb(V) transport. In summary, this study investigates the influence of several genes on plant tolerance and uptake to Sb(V) and elucidates the crucial role of the Pht1;4 gene, shedding light on the development of Sb phytoremediation strategies and Sb-resistant plants.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118308"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis","authors":"Huan Mao ,&nbsp;Yidan Wei ,&nbsp;Cheng Liu ,&nbsp;Jianmin Yang ,&nbsp;Liyu Su ,&nbsp;Yuyan Cen ,&nbsp;Feng Wang ,&nbsp;Deyu Zhu ,&nbsp;Yu Deng ,&nbsp;Yan Li","doi":"10.1016/j.ecoenv.2025.118266","DOIUrl":"10.1016/j.ecoenv.2025.118266","url":null,"abstract":"<div><div>Overexposure manganese can induce neurotoxicity and apoptosis, in addition, miRNAs are widely involved in the apoptotic process of neurons. Therefore, it is crucial to explore the mechanism of miRNAs in manganese-induced neuronal apoptosis and then to find potential targets. Our present study showed that miR-143 expression was increased in striatum after exposure high doses of manganese. Then, we upregulated miR-143 expression via stereotaxic, and overexpression of miR-143 promoted apoptosis in striatal neurons. Further studies showed a negative regulatory relationship between miR-143 and ERK5, miR-143 upregulation decreased ERK5 mRNA level, inhibiting ERK5 signaling pathway, increasing the expression of Bax and Caspase-3, and exacerbating the apoptosis of striatal neurons. In conclusion, these results suggest that overexpression of miR-143 inhibited the ERK5 signaling pathway and promoted manganese-induced apoptosis in striatal neurons.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118266"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics reveals the toxicological effects of penconazole (PEN) in mice oocyte","authors":"Dongyu Yang,&nbsp;Shan Lu,&nbsp;Jian Liu,&nbsp;Sinan Li,&nbsp;Xulong Chen,&nbsp;Yan Xie","doi":"10.1016/j.ecoenv.2025.118319","DOIUrl":"10.1016/j.ecoenv.2025.118319","url":null,"abstract":"<div><div>Penconazole (PEN) is a widely employed agent in the mitigation of fungal infestations, and recent research shows that water exposure and subsequent bioaccumulation within the food web elicits inflammatory responses in certain vital organs. Nonetheless, the fundamental pathways through which PEN impacts the quality of oocytes remain inadequately elucidated to date. We gavaged mouse with PEN (2.5 mg / kg) at the same time for 10 consecutive days and discovered a precipitous decline in the developmental proportion of oocytes collected from mice exposed to PEN. Metabolomic profiling revealed significant perturbations in key metabolic pathways, including oxidative phosphorylation and longevity regulation, with nicotinamide adenine dinucleotide (NAD) being markedly downregulated in PEN-exposed oocytes. Subsequent evaluations indicated a compromised state in both the cytoplasmic and nuclear maturation processes. This phenomenon stems from heightened levels of reactive oxygen species coupled with diminished mitochondrial functionality. Furthermore, Annexin-V signaling suggested that PEN triggered an apoptotic response in the oocytes. Our metabolomic findings underscore the central role of NAD depletion in mediating PEN-induced toxicity, linking mitochondrial dysfunction and oxidative stress to impaired oocyte quality. Overall, we recommend that females who are preparing for pregnancy in areas with higher risks of PEN exposure should supplement with Nicotinamide mononucleotide (NMN) to protect the quality of their oocytes.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118319"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-occupational exposure to cadmium and breast cancer: A comprehensive and critical review","authors":"Radomir Aničić ,&nbsp;Milica Zeković ,&nbsp;Milan Kocić ,&nbsp;Zoran Gluvić ,&nbsp;Dragan Manojlović ,&nbsp;Janez Ščančar ,&nbsp;Aleksandar Stojsavljević","doi":"10.1016/j.ecoenv.2025.118331","DOIUrl":"10.1016/j.ecoenv.2025.118331","url":null,"abstract":"<div><div>Breast cancer (BC) is a multifactorial disease with unresolved etiology. Environmental pollutants, primarily trace metals, play a pivotal role in the pathophysiological cascade of malignant tumors, including BC. In this up-to-date review, we comprehensively and critically examined the relationship between cadmium (Cd) and BC. For this purpose, peer-reviewed studies from relevant databases (PubMed, SCOPUS, and Cochrane Library) over the last 40 years were retrieved and analyzed. We found that <em>in vitro</em> and <em>in vivo</em> studies strongly support the view that Cd has harmful effects on breast health. According to the human studies, we found that Cd could be responsible for the development and progression of malignant breast tumors due to markedly higher levels in clinical matrices of cases (whole blood, urine, breast tissue, keratin materials) than in clinical matrices of controls. Cadmium does not appear to affect BC density. In contrast, Cd has been found to have a detrimental effect on sex hormones, disrupting the balance of estrogen and androgen. We found that studies looking at dietary Cd intake and BC risk generally (without measuring urine or blood Cd) do not support the association between dietary Cd intake and BC risk. In notable contrast, studies looking at dietary Cd intake and BC risk by measuring Cd in urine or blood generally support this association. The effect of airborne Cd on BC risk was weak, but in favor of specific histological forms, primarily ER-/PR- invasive tubular breast carcinomas. Regardless of the intake route of Cd into the body, it can be concluded that Cd has a harmful effect on breast health. However, well-designed longitudinal, mechanistic, meta-analytic, and other studies are urgently needed to confirm the exact role of environmental Cd in breast carcinogenesis.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118331"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thallium-induced neurocardiotoxicity in zebrafish: Protective role of adaptive UPR and DNA repair","authors":"Yung Chang ,&nbsp;Chia-Hsien Wu ,&nbsp;Jia-Huang Chen ,&nbsp;Tsuyoshi Inoue ,&nbsp;Chih-Kang Chiang","doi":"10.1016/j.ecoenv.2025.118321","DOIUrl":"10.1016/j.ecoenv.2025.118321","url":null,"abstract":"<div><div>Thallium (Tl) is a hazardous heavy metal widely used in industrial applications, leading to significant environmental contamination. Tl concentrations in surface waters can reach up to 1520 μg/L, exceeding safe limits and posing risks to aquatic ecosystems and human health. Monovalent thallium [Tl(I)] is highly stable and bioaccumulative, readily accumulating in aquatic organisms, plants, and the human food chain. Exposure to Tl has been associated with neurotoxicity, kidney dysfunction, and cardiovascular diseases, particularly affecting children and pregnant women, and may increase the risk of neurodegenerative diseases and cardiac arrhythmias. However, effective strategies to mitigate Tl toxicity remain lacking. This study establishes a zebrafish embryo model to investigate the toxicological mechanisms of Tl and evaluate the protective effects of IXA4, a selective XBP1 activator. Our results show that Tl exposure increases mortality, reduces hatching rates, impairs swim bladder development, and causes pericardial edema and brain abnormalities. Transcriptomic and qPCR analyses confirm that Tl induces endoplasmic reticulum (ER) stress and activates the unfolded protein response (UPR), key pathways involved in cellular toxicity. Co-treatment with IXA4 significantly improves survival rates and developmental outcomes by upregulating DNA repair genes, particularly in the nucleotide excision repair (NER) pathway, thereby reducing cardiac and neural damage. This study provides novel insights into the mechanisms of Tl toxicity, underscores the urgent need for stricter regulatory measures, and highlights IXA4 as a potential intervention for mitigating heavy metal toxicity in aquatic ecosystems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118321"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic exposure to polyethylene terephthalate microplastics induces gut microbiota dysbiosis and disordered hepatic lipid metabolism in mice","authors":"Tao Lu ,&nbsp;Huan Liu ,&nbsp;Xiaoqing Yuan ,&nbsp;Desheng Li ,&nbsp;Guanqing Zhang ,&nbsp;Yue Wang ,&nbsp;Qiqiang Xie ,&nbsp;Xinhao Wang ,&nbsp;Jingjian Chi ,&nbsp;Zhenzhen Wang ,&nbsp;Shaoping Wang ,&nbsp;Yanling Gao ,&nbsp;Ling Zhou ,&nbsp;Maolei Xu","doi":"10.1016/j.ecoenv.2025.118330","DOIUrl":"10.1016/j.ecoenv.2025.118330","url":null,"abstract":"<div><div>Despite the widespread presence of polyethylene terephthalate microplastics (PET MPs) in the environment, their biotoxicity, target organs, and underlying toxicological mechanism remain poorly understood. In this study, irregularly shaped PET MPs resembling those commonly found in natural environment were selected. Mice were orally administered different amounts of PET MPs (0, 5, 50, and 500 μg/day) for 17 weeks, after which relevant pathological and biochemical indicators were assessed. The results confirmed, for the first time, that PET MPs can induce oxidative stress, lipid accumulation, and apoptosis in liver cells, resulting in structural damage and functional abnormalities in the liver. Additionally, metabolomic analysis was combined with intestinal microbiota profiling to elucidate the potential toxicological mechanism. The data revealed that chronic exposure to high doses of PET MPs substantially altered the diversity of the intestinal flora. In particular, the relative abundances of <em>Parasutterella</em>, <em>Muribaculum</em>, and <em>Turicibacter</em> increased, accompanied by elevated levels of lipid metabolites such as linoleic acid, taurocholic acid, and sphingosine. These changes disrupted metabolic processes and accelerated lipid deposition in the mouse liver, thereby inducing hepatotoxicity. Moreover, a validation experiment confirmed that depletion of the gut microbiota in mice alleviated PET MPs-induced hepatotoxicity. These findings provide new insights into the toxicity of PET MPs in humans and other mammals.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118330"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water regime alters microbial mechanisms of N2O emission in metal-contaminated paddy soils","authors":"Yifan Guo ,&nbsp;Shulan Cheng ,&nbsp;Huajun Fang ,&nbsp;Jing Geng ,&nbsp;Fangying Shi ,&nbsp;Hui Wang ,&nbsp;Long Chen ,&nbsp;Haiguang Pu ,&nbsp;Bingqian Liu ,&nbsp;Yi Zhou","doi":"10.1016/j.ecoenv.2025.118304","DOIUrl":"10.1016/j.ecoenv.2025.118304","url":null,"abstract":"<div><div>Microorganisms are essential for soil nitrous oxide (N₂O) emissions through participating in key nitrogen (N)-related processes. However, the effect of water regimes on the interactions between N₂O emissions and microbial processes in metal-contaminated soils is unclear. Here, we conducted a soil microcosm experiment with two water management strategies (non-flooding and flooding) and six metal addition treatments including low (2 and 200 mg kg⁻¹) and high (10 and 1000 mg kg⁻¹) levels of individual and combined Cd and Cu. The effects of high levels of individual Cd and Cu contamination on soil N₂O emissions varied depending on water regimes, showing antagonistic effects under non-flooding conditions and synergistic effects under flooding conditions. High levels of co-contamination significantly inhibited nitrification under both water regimes, primarily due to reduced abundance of <em>Nitrosospira</em>. In contrast, this co-contamination decreased the abundance of <em>Ramlibacter</em>, thereby inhibiting denitrification and dissimilatory nitrate reduction to ammonium (DNRA) under flooding conditions. The inhibition of these key microorganisms and their mediated N-cycle processes reduced soil N₂O emissions under both water regimes. This reduction was greater under flooding conditions because more N-related processes were inhibited. Metagenomic binning further indicated that <em>Nitrosospira</em> carried nitrifying genes, while <em>Ramlibacter</em> contained genes involved in denitrification, assimilatory nitrate reduction to ammonium (ANRA), and DNRA. These findings implied that both microorganisms had potential to produce N₂O. Overall, water management strategies and metal contamination altered the microbial processes of N₂O emissions, highlighting the importance of appropriate water management in mitigating greenhouse gas emissions from metal-contaminated paddy soils in southern China.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118304"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy","authors":"Piuque M. Rodriguez ,&nbsp;Berta Vera ,&nbsp;Carolina Burgos ,&nbsp;Gustavo Gimenez ,&nbsp;Karina S.B. Miglioranza ,&nbsp;Cristina L. Ramirez ,&nbsp;Andrea Lavalle ,&nbsp;Paola M. Ondarza ,&nbsp;Natalia L. Guiñazú","doi":"10.1016/j.ecoenv.2025.118285","DOIUrl":"10.1016/j.ecoenv.2025.118285","url":null,"abstract":"<div><div>Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118285"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personal care products as a potential source of aquatic pollution: Effect of polyvinyl alcohol on physiological and antioxidant responses in Mytilus galloprovincialis","authors":"Cristiana Roberta Multisanti ,&nbsp;Giorgia Zicarelli ,&nbsp;Alessia Caferro ,&nbsp;Mariacristina Filice ,&nbsp;Jana Blahova ,&nbsp;Irene Vazzana ,&nbsp;Giuseppe Piccione ,&nbsp;Sandra Imbrogno ,&nbsp;Caterina Faggio ,&nbsp;Federica Impellitteri","doi":"10.1016/j.ecoenv.2025.118336","DOIUrl":"10.1016/j.ecoenv.2025.118336","url":null,"abstract":"<div><div>A high percentage of the broad spectrum of contaminants in aquatic environments is represented by personal care products (PCPs). Polyvinyl alcohol (PVA) is contained in the formulation of many of these products. The extensive use of PVA caused the annual release of over 65.000 tons into the environment, constituting 1.2 % of particles in the Mediterranean Sea. For these reasons, the study aimed to investigate, for the first time in bivalve species, the possible cytotoxicity implications on non-target species, involved in the human food chain, caused by exposure to two different concentrations of PVA (PVA1: 0.1 mg L⁻¹ and PVA2: 10 mg L⁻¹). Following a fortnight of exposure, cell viability, regulation of cell volume decrease, oxidative stress, and biochemical parameters were evaluated. Moreover, the capability of the animal to produce byssal plaques was observed, representing a novelty for assessing the health status of mussels. The results showed a modification in the regulatory capacity of cell volume, particularly in those exposed to PVA2. An alteration in the superoxide dismutase activity (in PVA2) and in the levels of oxidatively modified proteins (PVA1 and PVA2) was detected in gills. A modification in the byssal plaque production following PVA exposure was also observed. These findings enrich the knowledge about the interaction between PVA and organisms, underscoring further research's need to elucidate the environmental implications.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"298 ","pages":"Article 118336"},"PeriodicalIF":6.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}