Ecotoxicology and Environmental Safety最新文献

{"title":"Pesticide residues: Bridging the gap between environmental exposure and chronic disease through omics","authors":"Lingling Cao ,&nbsp;Qiyuan Kang ,&nbsp;Yuan Tian","doi":"10.1016/j.ecoenv.2024.117335","DOIUrl":"10.1016/j.ecoenv.2024.117335","url":null,"abstract":"<div><div>Pesticide residues, resulting from agricultural practices, pose significant health and environmental risks. This review synthesizes the current understanding of pesticide impacts on the immune system, highlighting their role in chronic diseases such as asthma, diabetes, Parkinson's disease (PD) and cancer. We emphasize the significant role of omics technologies in the study of pesticide toxicity mechanisms. The integration of genomics, proteomics, metabolomics, and epigenomics offers a multidimensional strategy for a comprehensive assessment of pesticide effects, facilitating personalized risk management and policy formulation. We advocate for stringent regulatory policies, public education, and global cooperation to enhance food safety and environmental sustainability. By adopting a unified approach, we aim to mitigate the risks of pesticide residues, ensuring human health and ecological balance are preserved.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117335"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PMMA nanoplastics induce gastric epithelial cellular senescence and cGAS-STING-mediated inflammation via ROS overproduction and NHEJ suppression","authors":"Xiao Li ,&nbsp;Yixing Huang ,&nbsp;Dan Zu ,&nbsp;Haidong Liu ,&nbsp;Hanyi He ,&nbsp;Qimei Bao ,&nbsp;Yanhua He ,&nbsp;Chen Liang ,&nbsp;Guoyan Luo ,&nbsp;Yaoshu Teng ,&nbsp;Yin Shi ,&nbsp;Zu Ye ,&nbsp;Xiangdong Cheng","doi":"10.1016/j.ecoenv.2024.117284","DOIUrl":"10.1016/j.ecoenv.2024.117284","url":null,"abstract":"<div><div>The increasing environmental presence of nanoplastics (NPs) has raised concerns about their potential impact on biological systems. We investigated the repercussions of polymethyl methacrylate (PMMA) NPs exposure on normal gastric epithelial cells and revealed a pronounced increase in senescence-associated β-galactosidase activity and G1 phase cell cycle arrest. Our study demonstrated a dose-dependent increase in reactive oxygen species (ROS) and DNA damage, underscoring the pivotal role of ROS in PMMA NPs-mediated effects, a novel contribution to the existing body of knowledge dominated by polystyrene particles. Furthermore, we explored the influence of PMMA NPs on DNA damage response mechanisms, highlighting the significant inhibition of nonhomologous end-joining (NHEJ). Our findings help to elucidate the consequent genomic instability, as evidenced by increased chromosomal aberrations and micronuclei formation. By connecting these cellular manifestations to organism-level effects, we hypothesize that PMMA NPs play a critical role in aging processes. Our work revealed an activated cGAS-STING signaling pathway after PMMA NPs exposure, which correlated with aging-related inflammation and behavioral changes in mice. Importantly, our study provides comprehensive evidence of PMMA NPs-induced premature aging in gastric epithelial cells, shedding light on the molecular intricacies underlying DNA damage, repair impairment, and inflammation. Our research prompts heightened caution regarding the risks of NPs exposure and calls for further investigation into the broader implications of these environmental pollutants on aging processes in higher organisms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117284"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juvenile hormone III improves honeybee resistance to imidacloprid by protecting the midgut","authors":"Nana Wang ,&nbsp;Kunrong Wang ,&nbsp;Li Lei ,&nbsp;Longtao Zhang ,&nbsp;Xingqi Guo ,&nbsp;Baohua Xu ,&nbsp;Ying Wang ,&nbsp;Chen Wang","doi":"10.1016/j.ecoenv.2024.117280","DOIUrl":"10.1016/j.ecoenv.2024.117280","url":null,"abstract":"<div><div>Imidacloprid is one of the most commonly used insecticides and seriously threatens the survival of honeybees. Juvenile hormone III (JHIII), an important hormone in honeybees, plays a central role in the life cycle of honeybees; however, the mechanism by which JHIII responds to imidacloprid stress has not been determined. In this study, the resistance of <em>Apis cerana cerana</em> (<em>Acc</em>) to imidacloprid was greater than that of <em>Apis mellifera</em> (<em>Am</em>), and the application of JHIII significantly increased the resistance of both species of honeybees. RT<img>qPCR revealed that two genes, <em>juvenile hormone acid methyltransferase</em> (<em>JHAMT</em>) and <em>methyl farnesoate epoxidase</em> (<em>MFE</em>), which are involved in JHIII biosynthesis, are key genes for improving resistance to imidacloprid via JHIII in honeybees. Silencing <em>JHAMT</em> and <em>MFE</em> with RNAi resulted in a lower content of JHIII in <em>Acc</em> and reduced resistance to imidacloprid. The exogenous application of JHIII compensated for the reduced resistance phenotype caused by gene silencing. Furthermore, histological examination of paraffin sections revealed that imidacloprid treatment caused loose intestinal cell arrangement, slight damage to columnar digestive cells and muscle cells, and chromatin condensation in basal layer cells. JHIII treatment reduced the degree of damage to the honeybee midgut cells and improved resistance to imidacloprid. The results of this study provide a new perspective and molecular basis for the study of the regulation of JHIII and the toxicity of imidacloprid in honeybees.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117280"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of growth performance and characterization of pathological lesions in long-term ammonia-exposed pigs","authors":"Byungkwan Oh ,&nbsp;Junsu Park ,&nbsp;Eunju Kim ,&nbsp;Siyoung Seo ,&nbsp;Bumseok Kim ,&nbsp;Sang-Ik Oh","doi":"10.1016/j.ecoenv.2024.117318","DOIUrl":"10.1016/j.ecoenv.2024.117318","url":null,"abstract":"<div><div>Ammonia (NH₃) is a major cause of odor emissions from swine farms, and exposure to high concentrations of NH₃ in short-term periods has been reported to cause respiratory and systemic disorders in pigs. However, the impact of long-term NH₃ exposure on pig health and productivity remain unknown. This study aimed to assess the impact of long-term NH₃ exposure on growth performance and pathological outcomes in pigs. Pigs were reared in the treatment room [34.8 mg/m³ NH₃ concentration (50 ppm); TRT group, <em>n</em> = 40] and the control room [5.6 mg/m³ (8 ppm); CON, <em>n</em> = 40]. The experimental period was 107 days (from weaning piglets to market age). Our findings revealed that long-term NH₃ exposure results in severe respiratory and systemic pathological lesions, including chronic tracheitis, epithelial metaplasia of the trachea, severe interstitial pneumonia, myocarditis, and interstitial nephritis. In addition, the TRT group exhibited reduced productivity, with two deaths, indicating that uncontrolled NH₃ concentrations on pig farms could be linked to a decline in growth performance and even death. Transcriptomic analysis revealed significant changes in immune and inflammatory pathways in lung from TRT pigs, including dysregulated cytokine signaling and incomplete inflammatory responses. The enrichment of DEGs in pathways, such as Cytokine-cytokine receptor interaction, JAK-STAT, and Toll-like receptor signaling, indicated chronic NH₃ exposure disrupted immune homeostasis, contributing to chronic inflammation and impaired tissue repair. In conclusion, this study provides valuable insights into the detrimental effects of NH₃ exposure on pig health and productivity under farm conditions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117318"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambient air pollution and hospital admission for interstitial lung diseases: A multicenter hospital-based case-crossover study","authors":"Yi Zheng ,&nbsp;Jun Liu ,&nbsp;Nongping Feng ,&nbsp;Jing Wei ,&nbsp;Xiaohong Jia ,&nbsp;Lu Luo ,&nbsp;Ruijun Xu ,&nbsp;Chunxiang Shi ,&nbsp;Rui Wang ,&nbsp;Hong Sun ,&nbsp;Yuewei Liu","doi":"10.1016/j.ecoenv.2024.117289","DOIUrl":"10.1016/j.ecoenv.2024.117289","url":null,"abstract":"<div><div>Interstitial lung diseases (ILDs) lead to increased morbidity and premature deaths, imposing a significant burden on public health worldwide. Recently, several studies have linked ambient air pollution with the acute exacerbation of certain ILDs, but the evidence remains limited and inconclusive. With a multicenter hospital-based case-crossover design, we investigated 9128 patients who resided in Jiangsu province, China, and were admitted for ILDs between 2019 and 2022. Residential exposure to particulate matter with an aerodynamic diameter ≤ 2.5 µm (PM_2.5), PM₁₀, sulfur dioxide (SO₂), nitrogen dioxide (NO₂), carbon monoxide (CO), and ozone (O₃) was assessed using our validated grid datasets. We fitted conditional logistic regression models to examine associations of exposure to air pollutants with ILD admission. A 10 µg/m³ increment of exposure to SO₂ and NO₂ was positively associated with a 16.18 % (95 % confidence interval [CI]: 3.79 %, 30.03 %) and 4.06 % (0.75 %, 7.49 %) increase in odds of ILD admission, respectively. All these associations appeared to be linear and the association of SO₂ exposure was significantly stronger among older adults. We estimated that over 10 % of ILD admissions could be attributable to exposure to SO₂ and NO₂. This study provides compelling evidence on the association of exposure to ambient air pollutants (including SO₂ and NO₂) with an increased odds of ILD hospitalizations. Our findings indicate that SO₂ and NO₂ exposures can lead to the exacerbation of ILDs, especially in elderly, and that the disease burden is considerable.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117289"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emamectin benzoate-induced stress significantly affects the gut microbiome of adult Zeugodacus cucurbitae","authors":"Ruwen Li ,&nbsp;Zhenya Tian ,&nbsp;Jingfang Yang ,&nbsp;Xuyuan Gao ,&nbsp;Hongsong Chen ,&nbsp;Yusha Wang ,&nbsp;Zhongshi Zhou","doi":"10.1016/j.ecoenv.2024.117312","DOIUrl":"10.1016/j.ecoenv.2024.117312","url":null,"abstract":"<div><div>The detoxification mechanisms in insects, which are triggered by insecticides, alter the diversity of their intestinal microorganisms. Emamectin benzoate is an insecticide used to control <em>Zeugodacus cucurbitae</em> (Coquillett), a globally significant pest. In this study, high-throughput sequencing, traditional isolation and culture methods, and single bacterial 16S rDNA sequencing were used to analyze the diversity and functional predictions of intestinal microbial communities in <em>Z. cucurbitae</em> adults exposed to emamectin benzoate. The results showed that the intestinal microorganisms of <em>Z. cucurbitae</em> on <em>Cucumis sativus</em> and <em>Benincasa hispida</em> var. chieh-qua were primarily composed of the phyla Proteobacteria and Bacteroidetes and genera <em>Providencia</em>, <em>Enterobacter</em>, <em>Citrobacter,</em> and <em>Klebsiella</em>. The relative abundances of <em>Citrobacter</em>, <em>Enterobacter</em>, <em>Klebsiella,</em> and <em>Raoultella</em> decreased with the induced stress, whereas those of <em>Providencia</em> and <em>Pectobacterium</em> increased. Diversity analysis revealed significant differences in the midgut flora of <em>Z. cucurbitae</em> before and after stress induction with emamectin benzoate.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117312"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal content in perch and rudd tissues and associated health risk assessment","authors":"Magdalena Stanek ,&nbsp;Bogusław Chachaj ,&nbsp;Szymon Ł. Różański","doi":"10.1016/j.ecoenv.2024.117303","DOIUrl":"10.1016/j.ecoenv.2024.117303","url":null,"abstract":"<div><div>Fish meat is recommended for consumption as a source of protein, essential unsaturated fatty acids, and fat-soluble vitamins, unfortunately it can also be a source of dangerous contaminants. The aim of the study was to assessment the level of Zn, Cu and Pb in water and bottom sediments collected in summer and autumn from the Koronowo Reservoir and the Wierzchucińskie Duże Lake and in the tissues of perch (<em>Perca fluviatilis</em> L.) and rudd (<em>Scardinius erythrophtalmus</em> L.) for health risk assessment of potential consumers. Metals accumulated in the tissues in the following order: meat, liver and gills: Zn &gt; Cu &gt; Pb. Of the four factors taken into account, such as: type of tissue, fish species, catching season and place of catching, the first one determined the degree of metal accumulation to the greatest extent. The high level of Pb in muscle tissue was determined by the content of this metal in tissue collected from rudd from Wierzchucińskie Duże Lake in the summer (0.383 mg kg⁻¹ ww). The bioconcentration factor (BCF) of Pb, Zn and Cu in all tissues of analysed fish was much higher from water than from sediments. The highest value of metal pollution index (MPI) in the meat (1.42), liver (12.97), and gills (8.49) was calculated for summer-caught fish. The research indicated that consumption of 100 g of fish meat could meet daily requirement for Zn up to 26.3 % and for Cu in 16.4 %. The potential risk of Pb consumption according to RDA is as much as 2.4 %.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117303"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal diesel particle exposure alters gut microbiota and induces lung injury in rat offspring","authors":"Chung-Ming Chen ,&nbsp;Yu-Chen S.H. Yang ,&nbsp;Hsiu-Chu Chou","doi":"10.1016/j.ecoenv.2024.117278","DOIUrl":"10.1016/j.ecoenv.2024.117278","url":null,"abstract":"<div><div>Maternal air pollutant exposure inhibits fetal lung development. Diesel exhaust particles (DEP) are one of the most substantial contributors to particulate matter pollution. The effects of maternal DEP exposure on gut microbiota in mothers and offspring and fetal lung development remain unclear. In this study, time-dated pregnant Sprague Dawley rats received intranasal administration of 100 μL phosphate-buffered saline (PBS) or DEP (250 μg) in 100 μL PBS from gestational days 16–21. The dams were permitted to deliver vaginally at term. On postnatal days 0 and 7, gut microbiota was sampled from the lower gastrointestinal tract. The right lung and terminal ileum were harvested for histological, cytokine, and 8-hydroxy-2′-deoxyguanosine (8-OHdG) analyses. On postnatal day 0, the dams exposed to DEP and rat offspring with maternal DEP exposure exhibited macrophages that phagocytized diesel particles and increased numbers of macrophages in the alveolar parenchyma. On postnatal days 0 and 7, the offspring of DEP-exposed dams exhibited significantly lower intestinal tight junction protein expression, higher lung 8-OHdG and cytokine levels, and substantial lung injury compared with the offspring of the control dams. No significant differences were observed in the microbiota composition and diversity between the control and DEP-exposed dams. Maternal DEP exposure altered the gut microbiota composition and diversity on postnatal days 0 and 7, with more significant effects observed in the offspring on postnatal day 7. Regarding the mechanism, lung injury in offspring may have been linked to altered gut microbiota communities and dysregulated metabolic pathways caused by maternal DEP exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117278"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to residential green space and semen quality among Chinese men","authors":"Tian Liang ,&nbsp;Yangchang Zhang ,&nbsp;Wangnan Cao ,&nbsp;Yufeng Li ,&nbsp;Qiang Zeng ,&nbsp;Shengzhi Sun","doi":"10.1016/j.ecoenv.2024.117309","DOIUrl":"10.1016/j.ecoenv.2024.117309","url":null,"abstract":"<div><h3>Background</h3><div>Residential greenness has been linked to various health benefits, but evidence on its association with sperm quality remains limited.</div></div><div><h3>Methods</h3><div>We enrolled 28,089 adult men from an infertility clinic in Hubei, China, from 2014 to 2020. Residential greenness was estimated using the Normalized Vegetation Index (NDVI) from satellite imagery, averaged within buffers of 250 m, 500 m and 1000 m around each participant’s residential address. We employed multivariate linear regression analysis to evaluate the association between NDVI exposure and semen quality, while controlling for individual characteristics and semen collection season. Additionally, we performed subgroup analyses to investigate potential variations in the association based on individual characteristics.</div></div><div><h3>Results</h3><div>An interquartile range increase of 0.243 in NDVI within the 1000 m buffer was associated with increases of 1.68 % (95 % CI: 0.31 %, 3.06 %) in sperm concentration, 0.43 % (95 % CI: 0.08 %, 0.79 %) in progressive motility, and 0.50 % (95 % CI: 0.14 %, 0.87 %) in total motility. These associations were consistent across different buffer sizes. The associations were more pronounced during the 70–90 lag days prior to semen collection (spermatogenesis stage) and among men aged ≥ 40 years or those with lower education levels.</div></div><div><h3>Conclusions</h3><div>Our study demonstrated that exposure to residential greenness may act as an innovative protective factor for semen quality.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117309"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phthalate exposure increases oxidative stress, early renal injury, and the risk of calcium urolithiasis: A case-control study","authors":"Shih-Ting Huang ,&nbsp;Tusty-Jiuan Hsieh ,&nbsp;Yung-Chin Lee ,&nbsp;Chia-Fang Wu ,&nbsp;Yi-Chun Tsai ,&nbsp;Chu-Chih Chen ,&nbsp;Sih-Syuan Li ,&nbsp;Jiun-Hung Geng ,&nbsp;Yu-Ming Hsu ,&nbsp;Che-Wei Chang ,&nbsp;Yau-Hsuan Tsau ,&nbsp;Shu-Pin Huang ,&nbsp;Yung-Shun Juan ,&nbsp;Wen-Jeng Wu ,&nbsp;Ming-Tsang Wu ,&nbsp;Chia-Chu Liu","doi":"10.1016/j.ecoenv.2024.117322","DOIUrl":"10.1016/j.ecoenv.2024.117322","url":null,"abstract":"<div><h3>Background</h3><div>Phthalates, ubiquitous in plastics and softening agents, are pervasive in our daily environment. Growing concerns have emerged regarding their potential impact on renal health, particularly due to their propensity to induce oxidative stress. However, the relationship between phthalate exposure and urolithiasis remains poorly understood. This research seeks to explore the connection between phthalate exposure, oxidative stress, and the risk of urolithiasis.</div></div><div><h3>Methods</h3><div>A case-control study involving 285 patients diagnosed with calcium urolithiasis and 594 healthy controls was conducted. Participants completed structured questionnaires and provided urine samples for measuring 10 phthalate metabolites, biomarkers of oxidative stress (malondialdehyde [MDA]) and early renal injury (N-acetyl-beta-D-glucosaminidase [NAG] and albumin/creatinine ratio [ACR]). For subsequent analyses, we utilized distinct categories: the sum of high-molecular-weight phthalate metabolites (∑HMWm), the sum of low-molecular-weight phthalate metabolites (∑LMWm), and the daily intake of di-2-ethylhexyl phthalate (DEHP)(DI_DEHP_5).</div></div><div><h3>Results</h3><div>Stone patients exhibited significantly elevated urinary biomarkers of oxidative stress (MDA) and early renal injury (NAG and ACR), along with higher levels of 9 out of 10 assessed phthalate metabolites compared to normal controls. Within the study population, significant positive associations were found between almost all individual phthalate metabolites and urinary biomarkers of oxidative stress (MDA) as well as early renal injury (NAG and ACR). Logistic regression further confirmed that elevated phthalate levels, including ∑HMWm, ∑LMWm, and DI_DEHP_5, were uniformly associated with an increased risk of oxidative stress, early renal injury, and urolithiasis after adjusting for confounding factors.</div></div><div><h3>Conclusions</h3><div>Our study uncovers a novel association between phthalate exposure and the risk of urolithiasis, underscoring the heightened risk of kidney injury posed by such exposure. Considering the widespread presence of phthalates, regulatory measures and public health interventions are crucial to mitigate phthalate-related nephrotoxicity, while further large-scale longitudinal research is imperative to validate our initial findings and elucidate the underlying mechanisms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117322"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}