Ecotoxicology and Environmental Safety最新文献

{"title":"Impact of tire particle leachates on microplankton communities in the Canary Islands","authors":"Marta Sampalo,&nbsp;May Gómez,&nbsp;Rodrigo Almeda","doi":"10.1016/j.ecoenv.2025.117787","DOIUrl":"10.1016/j.ecoenv.2025.117787","url":null,"abstract":"<div><div>Tire wear particles (TWP) are a major source of microplastics in the environment. Despite their prevalence, the effects of tire particle leachates on marine microplankton communities remains poorly understood. In this study, we assessed the acute impacts of tire particle leachates on the structure of coastal microplankton assemblages from the Canary Islands. Five laboratory experiments were conducted, exposing microplankton to a range of leachate dilutions over 72 h, with TWP leachates prepared from an initial concentration of 1 g L⁻¹ .Our results revealed that the abundances of diatoms, most dinoflagellates, and ciliates were significantly reduced following exposure to leachates, with median effective concentrations (EC₅₀) ranging from 30 to 660 mg L⁻¹ depending on the plankton community. Interestingly, <em>Ostreopsis cf. ovata,</em> a harmful algal bloom (HAB)-forming species, exhibited relatively high tolerance to tire particle leachates compared to other microplankton. Compared to other marine biota, ciliates appear to be most vulnerable plankton group to tire particle leachates (EC₅₀ = 30 and 146 mg L⁻¹). The higher tolerance of <em>O. cf. ovata</em> to pollution compared to other phytoplankton species (resource competitors), in combination with other factors, may contribute to the rise of HABs in polluted coastal areas. Although field data on TWP are limited, the observed negative effects on microplankton occurred at environmentally relevant concentrations. Our results indicate that TWP pollution can significantly impact marine planktonic communities, highlighting the urgent need to reduce TWP emissions and develop less toxic tire rubber additives.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117787"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications","authors":"Jiayi He ,&nbsp;Huiqian Zhang ,&nbsp;Hehua Quan ,&nbsp;Qingyuan Wang ,&nbsp;Congcong Wen ,&nbsp;Yiyan Wang ,&nbsp;Yang Zhu ,&nbsp;Ren-Shan Ge ,&nbsp;Xiaoheng Li","doi":"10.1016/j.ecoenv.2025.117847","DOIUrl":"10.1016/j.ecoenv.2025.117847","url":null,"abstract":"<div><div>As an alternative compound of bisphenol A (BPA), bisphenol B (BPB) was widely used in plastic materials. The potential actions of BPB on the function of Leydig cells through the regulation of H3K27me3 and H3K9me3 remains unclear. Our goal was to assess how BPB influences Leydig cell function via histone modifications mediated by H3K27me3 and H3K9me3. Male 56-day-old Sprague-Dawley rats were given with 0, 50, 100, and 200 mg/kg/day of BPB by the oral administration for 14 days to study the impact of BPB on the function of Leydig cells in rats. The findings indicated that BPB significantly reduced the serum testosterone levels at the dose of 100 mg/kg and 200 mg/kg and follicle-stimulating hormone levels at the doses of 50, 100, and 200 mg/kg, while increasing estradiol levels at the dose of 200 mg/kg. BPB did not alter the numbers of CYP11A1⁺ Leydig cells and SOX9⁺ Sertoli cells, but it downregulated the expression of key genes in testosterone synthesis pathway (<em>Lhcgr, Scarb1, Star, Cyp11a1, Cyp17a1, Hsd11b1, Hsd17b3,</em> and <em>Insl3</em>) and their corresponding protein levels. Notably, BPB significantly boosted the expressions of histone methylation markers like EEF1A1, SUZ12, EED, EZH2, H3K27me3, and H3K9me3 <em>in vivo</em>. H3K27me3 and H3K9me3 levels were enhanced at the proximal promoters of <em>Lhcgr</em>, <em>Cyp11a1</em>, and <em>Star</em> through ChIP and PCR analyses. Furthermore, adult Leydig cells were extracted and cultured with BPB (0, 10, 50, 100, and 200 μM) alone or in combination with H3K27me3 antagonist GSK-J4. The results demonstrated that BPB significantly decreased testosterone output, which was counteracted by GSK-J4 to reverse BPB-mediated testosterone suppression. Additionally, BPB significantly elevated the levels of EEF1A1, EEF1A2, EED, H3K27me3, and H3K9me3 <em>in vitro</em>. BPB could potentially hinder the growth and function of Leydig cells by modulating H3K27me3 and H3K9me3. The findings of the study indicate the involvement of histone methylation (H3K27me3) in BPB-induced steroidogenic dysfunction, emphasizing the correlation between histone modifications and male reproductive toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117847"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143279186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice","authors":"Xian’an Yu ,&nbsp;Qiuqin Zhou ,&nbsp;Xin-Yuan Huang ,&nbsp;Mingxue Chen ,&nbsp;Jiawen Zhou ,&nbsp;Tong Zhou ,&nbsp;Pengjie Hu ,&nbsp;Xia Pan ,&nbsp;Miaomiao Cheng ,&nbsp;Yongming Luo ,&nbsp;Peter Christie ,&nbsp;Longhua Wu","doi":"10.1016/j.ecoenv.2025.117863","DOIUrl":"10.1016/j.ecoenv.2025.117863","url":null,"abstract":"<div><div>Low-cadmium (Cd)-accumulating rice (<em>Oryza sativa</em> L.) cultivated by <em>OsNRAMP5</em> mutation is a promising approach for ensuring food safety and human health. However, the impacts of reduced manganese (Mn) uptake caused by <em>OsNRAMP5</em> mutation on rice growth and Cd accumulation under varying Mn levels remains unknown. Here, hydroponics and pot experiments were used to investigate the growth and Cd accumulation of an <em>OsNRAMP5</em> mutant rice, Zhong’an 7 (ZA7), and the wild type, Zhongzao 35 (ZZ35), under different Mn supply conditions, and the Mn thresholds for ZA7 were determined. There was lower biomass of ZA7 than ZZ35 under hydroponic conditions with 0–0.05 μM Mn supply, and this was alleviated at Mn concentrations ranging from 1 to 200 μM. In pot Experiment I the grain yields of ZA7 in five soils increased from 5.92 to 15.0 g pot⁻¹ as the inherent soil available Mn ranged from 3.17 to 400 mg kg⁻¹. In pot Experiment II the exogenous MnSO₄ significantly increased ZA7 grain yields by 135–173 % and reduced grain Cd concentrations by 50.3–72.9 % (<em>p</em> &lt; 0.05). Furthermore, both hydroponic and pot experiments show that Cd concentrations in ZA7 were significantly lower than those in ZZ35, and decreased further with increasing Mn supply. Finally, the relationship between soil available-Mn (diethylene-triamine-pentaacetic acid-extractable, DTPA-Mn), which is directly related to plant Mn uptake, and ZA7 grain yields fitted well. The optimum DTPA-Mn content for maximum yield of ZA7 occurred at 263 mg kg⁻¹ and the minimum DTPA-Mn threshold ensuring 90 % of the optimum grain yield was 165 mg kg⁻¹. Thus, the results demonstrated the greater Mn-deficiency sensitivity and higher Mn requirement of ZA7 compared to ZZ35, shedding new light on the wider application of <em>OsNRAMP5</em> mutant rice under different Mn conditions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117863"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling","authors":"He-Fei Sun,&nbsp;Xin-Nan Wang,&nbsp;Ya-Nan Li,&nbsp;Lan-Lan Wang,&nbsp;Yue-Ying Li,&nbsp;Lian-Ju Ma,&nbsp;Xue-Mei Li","doi":"10.1016/j.ecoenv.2025.117872","DOIUrl":"10.1016/j.ecoenv.2025.117872","url":null,"abstract":"<div><div>Endophyte can improve plant resistance to Pb stress, and long non-coding RNAs (lncRNAs) have been proved to play a vital role in response to environmental stress. However, there are few studies on the role of lncRNAs induced by endophyte in host plants under Pb stress. Therefore, we conducted high-throughput sequencing on root tissue of endophyte-infected and -uninfected rice seedlings under Pb stress, and analyzed the target genes of differentially expressed lncRNAs (DElncRNAs). The results showed that endophyte infection significantly increased plant height, above-ground fresh weight and dry weight, but significantly decreased root length and under-ground dry weight after 5 d of treatment. A total of 413 DElncRNAs (167 down-regulated and 246 up-regulated) were screened. Their target differentially expressed mRNAs (DEmRNAs) were significantly enriched in glutathione metabolism, plasma membrane and mineral elements transfer etc. DEmRNAs were most significantly enriched in glutathione metabolism, thereinto detected total glutathione, reduced and oxidized glutathione contents, glutathione S-transferase and glutathione reductase activities were significantly increased after 5 d of treatment. DElncRNAs and DEmRNAs were combined with miRNA database to construct ceRNA network. DEmRNAs in ceRNA network were mainly participated in carbohydrate metabolic process, peroxidase activity and phenylpropanoid biosynthesis. This study will help to understand the molecular mechanism elicited by endophytic infection within rice seedlings under Pb stress from the perspective of lncRNA.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117872"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological relationship between PM2.5 exposure and acute exacerbations of chronic obstructive pulmonary disease","authors":"Anfeng Cui ,&nbsp;Yingying Shao ,&nbsp;Yang Wang ,&nbsp;Haodong Wang ,&nbsp;Jiayi Song ,&nbsp;Caiji Liu ,&nbsp;Ningning Shen ,&nbsp;Zhiqing Yang ,&nbsp;Wangliang Zhang ,&nbsp;Tiane Luo ,&nbsp;Chen Wang","doi":"10.1016/j.ecoenv.2025.117846","DOIUrl":"10.1016/j.ecoenv.2025.117846","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates the association between fine particulate matter (PM2.5) exposure and acute exacerbations of chronic obstructive pulmonary disease (AECOPD).</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 1284 AECOPD hospitalizations at Shanxi Medical University's Second Hospital from 2018 to 2022. This data was paired with Shanxi's average monthly PM2.5 data from the Tracking Air Pollution in China (TAP) dataset. Correlations between PM2.5 levels and AECOPD clinicopathological features were assessed using ANOVA and Spearman’s correlation.</div></div><div><h3>Results</h3><div>Higher PM2.5 concentrations were observed during February-April and November-January, correlating with increased AECOPD admissions (<em>r</em> = 0.754), outpatient visits (<em>r</em> = 0.799), emergency visits (<em>r</em> = 0.447), and ICU transfers (<em>r</em> = 0.860). Among the patients, 76.5 % were smokers and 68.4 % were over 60 years old. Inflammatory markers (leukocyte and neutrophil counts, eosinophil percentages, C-reactive protein), D-dimer levels, PaCO2, and pulmonary fibrosis indicators showed positive correlations with PM2.5 levels, whereas Pulmonary Function measures (FEV1/ FVC, FEV1 %) and PaO2 showed inverse correlations.</div></div><div><h3>Conclusion</h3><div>Elevated PM2.5 exposure significantly increases the risk of AECOPD, linked to heightened inflammation, pulmonary fibrosis, and diminished pulmonary function, and the majority of these patients are over 60 years old or are smokers. This study provides valuable theoretical and practical insights for the proactive prevention and management of AECOPD in clinical settings.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117846"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficient prediction of inflammatory osteolysis caused by polylactic acid through network toxicology and molecular docking strategy","authors":"Zichen Cui ,&nbsp;Tian He ,&nbsp;Shuo Zhang","doi":"10.1016/j.ecoenv.2025.117876","DOIUrl":"10.1016/j.ecoenv.2025.117876","url":null,"abstract":"<div><div>Polylactic acid (PLA), as a bioplastic, is extensively utilized in bone tissue engineering for its biocompatibility, adaptability and affordability. However, the toxicological research of PLA is still limited. The hydrolysis products of PLA induced inflammatory response which caused inflammatory osteolysis mediated by oxidative damage through the recruitment of macrophages and the accumulation of foreign body multinucleated giant cells, ultimately leading to the failure of bone tissue regeneration. The lack of effective treatments highlights the importance of finding new therapies. This study systematically investigated the potential molecular mechanisms of PLA-induced inflammatory osteolysis by employing network toxicology and molecular docking techniques. We first conducted a network toxicology-based assessment according to the molecular structure of PLA. The result from integrating and screening targets from multiple databases identified 126 potential targets associated with PLA-induced inflammatory osteolysis, and then an interaction network diagram of the targets was constructed. Gene ontology (GO)/KEGG enrichment analysis clarified that PLA may cause inflammatory osteolysis via metabolic pathways and pathways in cancer, as well as lipid and atherosclerosis. Further analysis by STRING and Cytoscape software screened 25 core targets including HSP90AA1, AKT1, SRC, STAT1 and FYN. We found that the enriched highly correlated pathways covered 18 of the 25 core targets, supporting the scientific hypothesis that PLA induces inflammatory osteolysis. Moreover, the results of molecular docking confirmed that PLA displayed a strong binding ability with the core targets and formed stable binding. Taken together, this study not only revealed the potential biological mechanism of PLA-induced inflammatory osteolysis, but also provided new evidence for the future prevention and treatment of PLA-induced inflammation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117876"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment","authors":"Li Zhang ,&nbsp;Wenjing Shen ,&nbsp;Zhixiang Fang ,&nbsp;Laipan Liu ,&nbsp;Ruizong Jia ,&nbsp;Jingang Liang ,&nbsp;Biao Liu","doi":"10.1016/j.ecoenv.2025.117873","DOIUrl":"10.1016/j.ecoenv.2025.117873","url":null,"abstract":"<div><div>The cultivation of genetically modified (GM) maize has aroused notable public concern related to the potential risks to soil fauna caused by the release of foreign proteins. In this study, the potential effects of cultivating the GM maize variety DBN9936, which exhibits the expression of the Cry1Ab and EPSPS proteins, on earthworms (<em>Eisenia fetida</em>) were evaluated under laboratory and field conditions. No significant differences in the survival, body weight, cocoon production, or hatching of earthworms fed GM or non-GM DBN318 maize were detected after three consecutive generations in a laboratory test. The enzymatic activity assay results revealed no significant differences in superoxide dismutase (SOD) or catalase (CAT) enzyme activity between the GM and non-GM maize varieties. Furthermore, exogenous Cry1Ab and EPSPS proteins were undetectable in the gut tissues of earthworms raised with GM maize straw. GM maize cultivation imposed no adverse effects on the species composition or density of soil earthworms in the two consecutive years during the field test, and the soil earthworm species, total number of earthworms and density of each earthworm species did not significantly differ between the GM maize and non-GM maize lines. On the basis of our findings, we concluded that the cultivation of the GM maize variety DBN9936 does not pose a risk to earthworms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117873"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing phenotypic and genotypic antibiotic resistance in bacillus-related bacteria isolated from biogas digestates","authors":"He Sun ,&nbsp;Jolanta J. Levenfors ,&nbsp;Christian Brandt ,&nbsp;Anna Schnürer","doi":"10.1016/j.ecoenv.2025.117859","DOIUrl":"10.1016/j.ecoenv.2025.117859","url":null,"abstract":"<div><div>Antibiotic resistance poses a significant public health challenge, with biogas digestate, a byproduct of anaerobic digestion (AD), presenting potential risks when applied as a biofertilizer. Understanding the actual resistance levels in digestate is crucial for its safe application. While many studies have investigated antibiotic resistance in AD processes using culture-independent molecular methods, these approaches are limited by their reliance on reference databases and inability to account for gene expression, leading to potential inaccuracies in resistance assessment. This study addresses these limitations by combining culture-independent whole-genome sequencing (WGS) with culture-dependent phenotypic testing to provide a more accurate understanding of antibiotic resistance in digestate. We investigated the phenotypic and genotypic resistance profiles of 18 antibiotic-resistant bacteria (ARB) isolated from digestates produced from food waste and animal manure. Resistance was assessed using WGS and Estrip testing across 12 antibiotics from multiple classes. This is the first study to directly compare phenotypic and genotypic resistance in bacteria isolated from digestate, revealing significant discrepancies between the two methods. Approximately 30 % of resistance levels were misinterpreted when relying solely on culture-independent methods, with both over- and underestimation observed. These findings highlight the necessity of integrating both methods for reliable resistance assessments. Additionally, our WGS analysis indicated low potential for transferability of detected ARGs among the isolated ARB, suggesting a limited risk of environmental dissemination. This study provides new insights into antibiotic resistance in digestate and underscores the importance of integrating methodological approaches to achieve accurate evaluations of resistance risks.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117859"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of variations in airborne microbiota on pneumonia infection: An exploratory study","authors":"Li Zhou ,&nbsp;Chenchen Song ,&nbsp;Lianlian Zhao ,&nbsp;Zhi Guo ,&nbsp;Yuhan Lei ,&nbsp;Yunlin Han ,&nbsp;Kai Gao ,&nbsp;Yanfeng Xu ,&nbsp;Zhiguang Xiang ,&nbsp;Baicun Li ,&nbsp;Jianguo Guo","doi":"10.1016/j.ecoenv.2025.117795","DOIUrl":"10.1016/j.ecoenv.2025.117795","url":null,"abstract":"<div><h3>Background</h3><div>Previous studies showed airborne bacteria affect pneumonia incidence, but specific impacts of bacterial communities on <em>Klebsiella pneumoniae</em> infection were unknown.</div></div><div><h3>Methods</h3><div>Five different ratios of bacterial community structures were randomly generated. Mice were divided into control, artificial bacterial community exposure, and corresponding <em>Klebsiella pneumoniae</em> challenge groups. Changes in body weight, blood parameters, pulmonary pathology, inflammatory factors, metabolomics, and fecal microbiota were analyzed.</div></div><div><h3>Results</h3><div>Different bacterial community exposures had varying degrees of influence on body weight, complete blood count, inflammatory factors, alveolar lavage fluid and plasma metabolome, as well as intestinal microbiota at baseline and after infection. Metabolomic analysis showed that microbial exposure affected both bronchoalveolar lavage fluid and plasma metabolomes, suggesting systemic effects of microbial exposure on the organism. Differences in the structure of artificial microbiota had inconsistent effects on both the baseline state and the post-infection state, hinting at crosstalk between microbial exposure and <em>Klebsiella pneumoniae</em> infection. KEGG pathway analysis unveiled possible molecular mechanisms underlying the overall impact of microbial exposure on the lungs and the body as a whole. In the intestinal microbiota, differences were found in composition at the phylum and genus levels. Spearman correlation analysis established potential correlations between intestinal microbiota and differential metabolites, suggesting a potential link within the lung-gut axis.</div></div><div><h3>Conclusion</h3><div>This study demonstrated the significant and systemic impact of air microbiota structure differences on health. Future research should explore the underlying mechanisms to enhance our understanding of the air-environment-health relationship and identify interventions for improving public health strategies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117795"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between plasma perfluoroalkyl substances and high-grade serous ovarian cancer overall survival: A nested case-control study","authors":"Wei-Yi Xing ,&nbsp;Fang-Hua Liu ,&nbsp;Dong-Dong Wang ,&nbsp;Jia-Ming Liu ,&nbsp;Wen-Rui Zheng ,&nbsp;Jia-Xin Liu ,&nbsp;Lang Wu ,&nbsp;Yue-Yang Zhao ,&nbsp;He-Li Xu ,&nbsp;Yi-Zi Li ,&nbsp;Yi-Fan Wei ,&nbsp;Dong-Hui Huang ,&nbsp;Xiao-Ying Li ,&nbsp;Song Gao ,&nbsp;Qi-Peng Ma ,&nbsp;Ting-Ting Gong ,&nbsp;Qi-Jun Wu","doi":"10.1016/j.ecoenv.2025.117825","DOIUrl":"10.1016/j.ecoenv.2025.117825","url":null,"abstract":"<div><h3>Background</h3><div>Although evidence suggests that perfluoroalkyl and polyfluoroalkyl substances (PFASs) are positively correlated to several disease risks, no studies have proven if plasma PFASs are related to ovarian cancer survival.</div></div><div><h3>Objective</h3><div>To explore the association between plasma PFASs and high-grade serous ovarian cancer (HGSOC) overall survival (OS) in the population who did not smoke.</div></div><div><h3>Methods</h3><div>We conducted a nested case-control study within the Ovarian Cancer Follow-Up Study, matching 159 dead patients and 159 survival ones based on body mass index, sample date, and age at diagnosis. Nine plasma PFASs were extracted by solid phase extraction and measured using a liquid chromatography system coupled with tandem mass spectrometry. Baseline plasma concentrations of perfluorinated carboxylic acids (PFCAs) [perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), and perfluoroheptanoic acid (PFHpA)] and perfluorinated sulfonic acids (PFSAs) [perfluorooctane sulfonic acid (PFOS) and perfluorohexane sulfonic acid (PFHxS)] were calculated. Odds ratios (ORs) and corresponding 95 % confidence intervals (CIs) were calculated via conditional logistic regression models. To elucidate the combined effects, Bayesian kernel machine (BKMR), and regression quantile g-computation (QGC) models were utilized.</div></div><div><h3>Result</h3><div>In full-adjusted model, significant differences were observed between HGSOC survival and perfluorobutane sulfonic acid, PFHpA, PFHxS, PFOS, PFCA, and PFSA. ORs and 95 %CIs were 2.74 (1.41–5.31), 1.97 (1.03–3.76), 2.13 (1.15–3.95), 2.28 (1.16–4.47), 3.74 (1.78–7.85), and 2.56 (1.31–5.01), respectively for the highest tertile compared with the lowest tertile. The QGC and BKMR models indicated that elevated concentrations of PFAS mixtures were associated with poor OS in HGSOC.</div></div><div><h3>Conclusions</h3><div>Both individual and mixed plasma PFASs may relate to poor OS of HGSOC. Further research is necessary to establish causality, and it is recommended to reinforce environmental risk mitigation strategies to minimize PFAS exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"291 ","pages":"Article 117825"},"PeriodicalIF":6.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}