Ecotoxicology and Environmental Safety最新文献

{"title":"Transcriptomics and stress indices reveal Tetraglyme-mediated adverse effects in the Mediterranean mussel Mytilus galloprovincialis","authors":"Nikolina Charalampous ,&nbsp;Stefanos Dailianis ,&nbsp;Georgios Kalamaras ,&nbsp;Maria Kamilari","doi":"10.1016/j.ecoenv.2025.119123","DOIUrl":"10.1016/j.ecoenv.2025.119123","url":null,"abstract":"<div><div>The present study provides the first integrated assessment of tetraethylene glycol dimethyl ether (Tetraglyme) on the Mediterranean mussel <em>Mytilus galloprovincialis</em>, by combining cellular, oxidative, and genotoxic stress indices along with transcriptomic profiling. Our findings represent the first molecular and physiological evidence of Tetraglyme-induced toxicity in a marine invertebrate. Mussels were exposed to environmentally relevant concentrations of Tetraglyme (1–1000 μg L⁻¹) for four days (Tetra-mussels) and showed increased hemocyte lysosomal membrane destabilization (in terms of lower neutral red retention time/NRRT values), higher frequencies of nuclear abnormalities (micronuclei and total nuclear abnormalities), and elevated oxidative-stress indices (NO₂^-, O₂^·- and MDA) at concentrations higher than 1 μg L⁻¹ . In parallel, transcriptome analysis of gill tissue revealed extensive differential expression of genes involved in cell homeostasis, metabolism, detoxification, cell signaling, apoptosis, and immune function. Notably, most high-confidence genes were downregulated, highlighting a pervasive molecular response and potential impairment of vital biological processes. Our results underscore the importance of multi-endpoint, omics-informed approaches for early detection of chemical threats to aquatic biodiversity and contribute novel mechanistic insight for the protection of aquatic ecosystems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119123"},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined phosphate-solubilizing bacteria and calcium-magnesium-phosphate fertilizer alleviate Cd toxicity and reduce Cd uptake in wheat: Insights into physiological responses and molecular mechanisms","authors":"Li Lu,&nbsp;Xihong Zhang,&nbsp;Endong Yang,&nbsp;Yeyu Zhu,&nbsp;Yuanyuan Cao,&nbsp;Leni Sun","doi":"10.1016/j.ecoenv.2025.119118","DOIUrl":"10.1016/j.ecoenv.2025.119118","url":null,"abstract":"<div><div>Cadmium (Cd) in the environment is toxic to plant growth and poses significant risks to human health through the food chain. However, research on the effects and mechanisms of combined application of phosphate-solubilizing bacteria (PSB) and calcium-magnesium-phosphate (CMP) fertilizer in alleviating Cd toxicity and reducing Cd uptake in wheat are lacking. In this study, we investigated the capacity of PSB <em>Burkholderia</em> sp. YM3 to dissolve CMP fertilizer, and evaluated the effects of its combined application with CMP on growth, physiological responses, Cd and phosphorus (P) uptake, and gene expression in wheat seedlings under Cd stress. Our results demonstrated that strain YM3 effectively solubilized CMP fertilizer, thereby releasing soluble calcium, magnesium, and P. Notably, the combined treatment (YM3 +CMP) presented the highest Cd removal efficiency in solution culture, reaching 88.79–90.55 %. This co-application also alleviated Cd-induced toxicity in wheat seedlings, as evidenced by significant reductions in the content of malondialdehyde (MDA:11.16–51.85 %), proline (44.04–49.33 %) and hydrogen peroxide (H₂O₂:44.94–61.93 %), as well as antioxidant enzyme activity. Furthermore, the combined treatment enhanced plant growth, root vigor (77.85–183.14 %), total chlorophyll content (40.79–78.75 %), and total P accumulation (9.23–86.76 %). Critically, YM3 +CMP also significantly reduced Cd uptake in both the aboveground tissues (51.39–54.80 %) and roots (49.73–50.34 %). At the molecular level, YM3 +CMP downregulated the expression of metal transporter genes <em>TaLCT1</em> and <em>TaNramp5</em>, while upregulating the expression of the zinc transporter gene <em>TaZIP3</em> and the P transporter genes <em>TaPHT1.2</em> and <em>TaPHT1.9</em> under Cd stress (<em>p</em> &lt; 0.05). These results indicate that the combined application of strain YM3 and CMP fertilizer has considerable potential for alleviating Cd-induced toxicity and reducing Cd uptake in wheat seedlings and will contribute to a deeper understanding of the molecular mechanisms underlying the response of wheat to the co-application of PSB and CMP under Cd stress.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119118"},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic evidence of Dicranopteris pedata’s specialized defense systems against REEs stress","authors":"Wenzhi Zhou ,&nbsp;Haiyan Wang ,&nbsp;Yonghe Han ,&nbsp;Yanglong Jiang ,&nbsp;Zhibiao Chen","doi":"10.1016/j.ecoenv.2025.119100","DOIUrl":"10.1016/j.ecoenv.2025.119100","url":null,"abstract":"<div><div><em>Dicranopteris pedata</em> is a typical REEs-hyperaccumulator that efficiently takes up REEs from the soil. However, the molecule mechanisms of REEs absorption and transportation by <em>D. pedata</em> is still unclear. In this study, <em>D. pedata</em> was selected as the research subject and exposed it to varying concentrations of yttrium (Y) and cerium (Ce) to investigate its stress effects. The results revealed, low exogenous REEs concentration can promote the expression of photosynthesis genes, and higher REEs stress can impede this process. Moreover, the significant down-regulation of PstS, ABCA3, ABCB1, ABCC1, and ABCD3 of the ABC transporter family may reduce the adverse effects of REEs. <em>D. pedata</em> can alleviate oxidative damage by increasing the activity of SOD and CAT, but this mechanism fails under high REEs stress. In addition, <em>D. pedata</em> up-regulates the gene expression of flavonoids, brassinosteroids, lysine, isoquinoline alkaloids, and streptomycin biosynthesis to alleviate stress. Besides, <em>D. pedata</em> also up-regulated the expression of naphthalene, caprolactam, toluene, geraniol, and fluorobenzoate degradation genes, which may be related to the detoxification mechanism of <em>D. pedata</em>. This study elucidates the physiological and transcriptional regulatory mechanisms of REEs stress response in <em>D. pedata</em>, deepening our understanding of the response of <em>D. pedata</em> to REEs stress.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119100"},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential trace element profile in follicular fluid and recurrent pregnancy loss: The role of amino acids—A case-control study","authors":"Xun Su ,&nbsp;Qian Li ,&nbsp;Yiqing Fan ,&nbsp;Weixuan Yang ,&nbsp;Meijie Luo ,&nbsp;Tao Yin ,&nbsp;Xinyu Yue ,&nbsp;Jiajing Tao ,&nbsp;Weiwei Zou ,&nbsp;Jie Sheng ,&nbsp;Chunmei Liang ,&nbsp;Dongmei Ji ,&nbsp;Yunxia Cao","doi":"10.1016/j.ecoenv.2025.119106","DOIUrl":"10.1016/j.ecoenv.2025.119106","url":null,"abstract":"<div><div>Recurrent pregnancy loss (RPL), defined as two or more consecutive pregnancy losses, imposes a significant burden on both individuals and healthcare systems. Imbalances in essential trace elements (ETEs) and dysregulated amino acid metabolism may contribute to RPL pathogenesis. However, the interplay between ETEs and amino acid metabolites in RPL remains largely unexplored. To address this gap, a case–control study including 69 women with a history of ≥ 2 unexplained RPL as cases and 148 healthy women as controls was performed. The levels of ETEs in follicular fluid (FF) were assessed using ICP-MS, and amino acids profiling was performed using an UHPLC system coupled with a Q-TOF mass spectrometer. Binary logistic regression was used to evaluate the association between each individual element with unexplained RPL risk, while qgcomp and BKMR models were used to assess the joint association between ETE mixtures and RPL risk. Mediation analysis was then performed to investigate the contribution of metabolites to the association between ETEs and unexplained RPL. The study finds that iron levels were positively related to RPL risk (medium vs. lowest tertile: adjusted OR (aOR) = 2.59, 95 % CI = 1.05, 6.39; highest vs. lowest tertile: aOR = 3.31, 95 % CI = 1.37, 8.03). Conversely, cobalt showed an opposite trend (highest vs. lowest tertile: aOR = 0.37, 95 % CI = 0.16, 0.86). In the mixture analysis, iron and cobalt emerged as the main drivers of RPL risk. Furthermore, mediation analysis revealed that L-alanine accounted for the most significant proportion (53.88 %) of the association between iron exposure and unexplained RPL risk. In conclusion, this study revealed that elevated iron levels are associated with altered amino acid metabolism and contribute to the risk of RPL, whereas cobalt exhibits an opposite association. Further research is required to elucidate this interplay.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119106"},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of greenness and air pollution with leukocyte telomere length in Chinese children and adolescents","authors":"Qing Liu ,&nbsp;Xiaoning Lei ,&nbsp;Wenning Fu ,&nbsp;Gaojie Fan ,&nbsp;Qing Fang ,&nbsp;Fei Luo ,&nbsp;Xiaofeng Huang ,&nbsp;Heng Li ,&nbsp;Wenwen Guo ,&nbsp;Binghai Liu ,&nbsp;Lianyan Yan ,&nbsp;Jing Wei ,&nbsp;Youjie Wang ,&nbsp;Lulu Song ,&nbsp;Liqin Hu","doi":"10.1016/j.ecoenv.2025.119122","DOIUrl":"10.1016/j.ecoenv.2025.119122","url":null,"abstract":"<div><div>Evidence on the associations of greenness and air pollution with telomere length (TL) in children and adolescents remains scarce, and the potential role of air pollution in the greenness-TL association is unclear. We aimed to evaluate the relationships of greenness and air pollution with leukocyte TL (LTL) in children and adolescents and further explore further explore the interaction and mediation of air pollution on the greenness-LTL association. In this cross-sectional study, 1151 children and adolescents aged 6–18 years were recruited from Liuzhou, China. Greenness (measured by the soil-adjusted vegetation index [SAVI], normalized difference vegetation index [NDVI], and enhanced vegetation index [EVI]) and air pollution (PM_2.5, PM₁₀, NO₂, SO₂, and CO) were estimated based on participants’ school and home addresses. Multiple linear regression models were employed to assess the associations of greenness and air pollution with LTL. An interquartile range (IQR) increment in SAVI_500 m was associated 3.32 % (95 % confidence interval [CI]: 1.17 %, 5.51 %) longer LTL, while an IQR increment in PM_2.5, PM₁₀, NO₂, SO₂, and CO were associated with 2.26 % (95 % CI: −4.32 %, −0.16 %), 5.20 % (95 % CI: −7.48 %, −2.85 %), 9.90 % (95 % CI: −13.33 %, −6.34 %), 7.64 % (95 % CI: −11.21 %, −3.91 %), and 6.61 % (95 % CI: −9.77 %, −3.35 %) shorter LTL, respectively. Interaction and mediation were identified for PM₁₀, SO₂, NO₂, and CO in the greenness-LTL association with mediation proportions from 37.90 % to 68.69 %. In conclusion, greenness exposure was positively associated with leukocyte telomere length in children and adolescents, and this association is primarily mediated by the attenuation of air pollution exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119122"},"PeriodicalIF":6.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-resolved exposure to fungi and bacteria, dust, and endotoxin during recycling of diverse waste types and systemic inflammatory response in workers","authors":"Anne Mette Madsen ,&nbsp;Hjördís Birna Árnadóttir ,&nbsp;Pieter Bertier ,&nbsp;Michael M. Tunney ,&nbsp;Harald Hannerz ,&nbsp;Steven Verpaele ,&nbsp;Margit Wagtberg ,&nbsp;Gísli G. Einarsson","doi":"10.1016/j.ecoenv.2025.119097","DOIUrl":"10.1016/j.ecoenv.2025.119097","url":null,"abstract":"<div><div>The waste recycling workforce is growing across Europe. This study investigates the airborne exposure of workers recycling plastic, paper/cardboard, and electronic (e) waste, and examines whether serum levels of inflammatory markers correlate with exposure. Exposure was measured repeatedly and analysed for inhalable and respirable dust, inhalable endotoxin, fungi, and bacteria. Microorganisms were identified using MALDI-TOF MS on cultured microorganisms and bacteria by 16S rRNA marker-gene sequencing. Blood samples collected at the end of each workday were analysed for three markers of inflammation. Waste types/tasks had an impact on exposure levels for all exposures and temperature on exposure to anaerobic bacteria. Exposure levels to dust, endotoxin, and anaerobic bacteria differed between workers. Exposures were highest for those handling paper/cardboard and plastic waste. The alpha diversity indices for most exposures did not differ between types of waste handled, but eWaste was associated with a lower species richness. Beta diversity did not differ between indoor temperatures or waste types except for mesophilic bacteria and bacteria (NGS-data). The species <em>Aspergillus niger, Penicillium brevicompactum</em>, <em>Bacillus cereus</em>, and <em>Staphylococcus equorum</em> were frequently detected. Serum levels of inflammatory markers increased with increasing exposure to dust, fungi or <em>Penicillium</em> spp, and anaerobic bacteria, but did not correlate with bacterial biodiversity indices. The study suggests further investigations of the impact of daily inhalation of bacteria able to grow anaerobically and fungi. Based on the high exposure levels, and the association between exposure and biomarkers of inflammation, it is advisable to explore risk management strategies aimed at minimizing worker exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119097"},"PeriodicalIF":6.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing cardiac insufficiency risks of aflatoxin B1 through network toxicology and molecular docking analyses","authors":"Qianyao Zhang ,&nbsp;Qing Zhang ,&nbsp;Piqiao Jiang ,&nbsp;Xiaotong Zhao ,&nbsp;Zhonghua Wen ,&nbsp;Yangyang Liu ,&nbsp;Qian Cao ,&nbsp;Siyao Wang","doi":"10.1016/j.ecoenv.2025.119095","DOIUrl":"10.1016/j.ecoenv.2025.119095","url":null,"abstract":"<div><div>This study aimed to elucidate the promoting effect of Aflatoxin B1 (AFB1) on cardiac insufficiency and its specific molecular mechanisms through methods including network toxicology, molecular docking, dynamics simulation, and in vivo experiments. A total of 44 overlapping targets between cardiac insufficiency and AFB1 toxicity were identified. Subsequently, a PPI network was constructed using STRING and Cytoscape software, and 12 core genes were identified, including AKT1, HIF1A, MMP9, PIK3CA, NFKB1, GSK3B, TLR4, KDR, JAK2, MAPK1, PIK3R1, and PTPN11. GO and KEGG enrichment analyses demonstrated that AFB1-induced cardiac insufficiency is primarily linked to the activation of the PI3K-AKT and MAPK signaling pathways. Molecular docking results showed that AFB1 exhibited strong binding affinity with all 12 core targets, among which its binding ability to JAK2, GSK3B, and MMP9 ranked as the top three. Molecular dynamics simulations focused on the complex formed between AFB1 and JAK2, confirming its favorable stability. Additional murine experiments confirmed that AFB1 exposure induces cardiac insufficiency and activates the PI3K-AKT and MAPK signaling pathways. These findings collectively demonstrate that AFB1 binding to JAK2 activates both the PI3K-AKT and MAPK pathways, consequently promoting cardiac insufficiency. By elucidating the underlying molecular mechanisms, this study establishes a theoretical framework for understanding how chronic AFB1 exposure aggravates cardiac insufficiency.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119095"},"PeriodicalIF":6.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Air pollutants, road traffic noise, and incidence of valvular heart disease: A UK Biobank-based prospective study","authors":"Yanjun Song ,&nbsp;Zhangyu Lin ,&nbsp;Zhen’ge Chang ,&nbsp;Jining He ,&nbsp;Zhihao Zheng ,&nbsp;Shanshan Shi ,&nbsp;Bowen Li ,&nbsp;Chen Zhu ,&nbsp;Rui Fu ,&nbsp;Kefei Dou","doi":"10.1016/j.ecoenv.2025.119104","DOIUrl":"10.1016/j.ecoenv.2025.119104","url":null,"abstract":"<div><div>Air and noise pollution are recognized public health concerns. However, their long-term impact on valvular heart disease (VHD) remains unclear. This study analyzed 453,413 participants from the UK Biobank. Air pollution was quantified using the air pollution score based on PM_2.5, PM_2.5–10, PM₁₀, NO_x, and NO₂ levels. Road traffic noise (L_den and L_nig_ht) exposure was estimated based on baseline residential addresses. Participants were categorized into quintiles (Q1-Q5) of exposure. VHD diagnoses were identified from hospitalization and cause-of-death records. Multivariable Cox proportional hazard models were used to assess the association between pollution exposures and VHD risk, presented as hazard ratios (HR) with 95 % confidence intervals (CI). During a median follow-up of 13.9 years, 18,506 cases of VHD were recorded. Participants exposed to the highest levels of pollution exposures had a significantly increased risk of developing VHD compared to those in the lowest exposure group (Q5 vs. Q1: air pollution score [HR 1.73, 95 % CI 1.61–1.87], PM_2.5 [HR 1.69, 95 % CI 1.57–1.82], PM_2.5–10 [HR 1.43, 95 % CI 1.34–1.53], PM₁₀ [HR 1.29, 95 % CI 1.22–1.36], NO₂ [HR 1.59, 95 % CI 1.47–1.71], NO_X [HR 1.64, 95 % CI 1.53–1.77], L_den [HR 1.66, 95 % CI 1.56–1.77], and L_night [HR 1.67, 95 % CI 1.57–1.78]). These associations persisted significantly regardless of the genetic risk to VHD and extended across all VHD subtypes, including non-rheumatic, rheumatic, aortic, mitral, tricuspid, and pulmonary valve diseases. This study demonstrates a significantly positive association between long-term exposure to air and noise pollution and increased VHD risk.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119104"},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disrupting manure-to-soil transmission of antibiotic resistance genes with gamma irradiation and hydrogen peroxide","authors":"Ruochen Li,&nbsp;Xin Pei,&nbsp;Chengyuan Tao,&nbsp;Xuhui Deng,&nbsp;Yanjie Wang,&nbsp;Ying Wang,&nbsp;Jiabao Wang,&nbsp;Zongzhuan Shen,&nbsp;Qirong Shen,&nbsp;Rong Li","doi":"10.1016/j.ecoenv.2025.119086","DOIUrl":"10.1016/j.ecoenv.2025.119086","url":null,"abstract":"<div><div>The application of untreated livestock manure poses significant risks for the dissemination of antibiotic resistance genes (ARGs) in agricultural ecosystems, yet conventional pretreatments such as composting or single chemical oxidation often fail to control both ARGs and their mobility. In this study, the synergistic effects of gamma irradiation and hydrogen peroxide were evaluated through integrated metagenomic analysis and soil microcosm experiments, showing that the combined treatment achieved the greatest reductions in both the abundance and diversity of ARGs and mobile genetic elements, outperforming individual treatments. Specifically, in the fertilizer treatment, the ARGs and mobile genetic elements in the combined treatment were reduced by 29.47 % and 24.25 %, respectively, compared with those in the control. The intervention selectively depleted ARG-harboring taxa, including <em>Cellvibrio</em>, <em>Alicyclobacillus</em>, and <em>Chitinophaga</em>, while concurrently suppressing microbial functions critical to ARG dissemination, particularly biofilm formation, membrane transport, and SOS stress response pathways. In soil microcosms, pretreated manure maintained ARG suppression via functional reconstruction, with microbial community shifts identified as the primary driver. These findings highlight a novel dual-action mechanism of gamma irradiation/H₂O₂ co-treatment that not only reduces ARG reservoirs but also disrupts ecological networks underpinning manure-to-soil ARG transmission, offering a mechanistically informed alternative to traditional manure management.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119086"},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between exposure to brominated flame retardants and gallstones risk in U.S. adults","authors":"Xiuquan Shi ,&nbsp;Yangyang Li ,&nbsp;Lei Jin ,&nbsp;Qiaoyu Xuan ,&nbsp;Qingsheng Yu","doi":"10.1016/j.ecoenv.2025.119113","DOIUrl":"10.1016/j.ecoenv.2025.119113","url":null,"abstract":"<div><h3>Background</h3><div>Gallstones, affecting 6–13.9 % of adults worldwide, have been associated with metabolic disturbances and environmental pollutants. Brominated flame retardants (BFRs), a class of persistent organic pollutants with hepatotoxic potential, have been hypothesized to contribute to gallstone pathogenesis; however, the extant epidemiological evidence remains inconclusive. This study aims to investigate the association between serum BFRs and gallstone risk, exploring nonlinear relationships, mixed exposures, and mediating mechanisms.</div></div><div><h3>Materials and methods</h3><div>From 15,560 participants in NHANES (2017–2020), 2026 individuals were included after applying exclusion criteria. Gallstone status was self-reported, and five serum BFRs with detection rates exceeding 75 % were analyzed. The analysis incorporated a range of covariates, including demographics, socioeconomic factors, and health-related variables. Statistical methods included weighted quantile sum (WQS) regression, quantitative g-computation (QGC), restricted cubic spline (RCS) models, and mediation analysis to assess nonlinearity, cumulative effects, and the role of the neutrophil-to-albumin ratio (NAR).</div></div><div><h3>Results</h3><div>Among the 2026 participants, 92 % reported alcohol consumption. Restricted cubic spline models revealed positive associations between gallstone risk and four BFRs: bis(1-chloro-2-propyl) phosphate, bis-2-chloroethyl phosphate, dibutyl phosphate, and diphenyl phosphate (P &lt; 0.05). WQS and QGC analyses indicated that mixed BFR exposure was positively associated with gallstones, with dibutyl phosphate contributing most strongly (weight = 0.55). Mediation analysis showed that NAR mediated 18.3 % of the effect of dibutyl phosphate on gallstones (P &lt; 0.001), but did not mediate associations for other BFRs.</div></div><div><h3>Conclusion</h3><div>Among the evaluated BFRs, dibutyl phosphate was most strongly associated with gallstone prevalence, potentially involving pathways related to neuromodulation and activation. Although these findings do not establish causality, they suggest that BFR exposure may increase the risk of gallstones.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"304 ","pages":"Article 119113"},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}