Ecotoxicology and Environmental Safety最新文献

{"title":"Exposure to mercury and polychlorinated biphenyls affects the thyroid function of an Australian seabird (Ardenna carneipes)","authors":"Dayanthi Nugegoda ,&nbsp;Stephen Raison ,&nbsp;Ana F. Miranda ,&nbsp;Jason Lu ,&nbsp;Chantel S. Foord ,&nbsp;Damien Nzabanita ,&nbsp;Jennifer L. Lavers","doi":"10.1016/j.ecoenv.2025.118501","DOIUrl":"10.1016/j.ecoenv.2025.118501","url":null,"abstract":"<div><div>As top predators in the marine environment, seabirds can be exposed to high levels of persistent pollutants that can bioaccumulate and biomagnify making these predators excellent indicators of ecosystem health. Commonly found in the marine environment, mercury (Hg) and polychlorinated biphenyls (PCBs) are known to interfere with the thyroid system in wildlife. This study quantified PCBs and Hg concentrations and investigated the relationship with thyroxine (T4) and triiodothyronine (T3) levels, in fledgling and adult sable shearwaters (<em>Ardenna carneipes</em>). Hg and PCBs were measured in feathers and red blood cells, respectively. The results indicate Hg and PCBs were more abundant in adult shearwaters than in fledglings. Negative associations were found between Hg/PCB body burdens and circulating thyroid hormone concentrations in both age categories. However, some of these correlations were not statistically significant. This study presents an empirical dataset of these contaminants and the thyroid function of adult and juvenile birds. This is a significant step towards better understanding the threat posed by Hg and PCBs to the health of seabirds.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118501"},"PeriodicalIF":6.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data mining-based screening of prevalent mixture systems in aquatic environments: A case study of antibiotics in the Yangtze River Basin","authors":"Ting-Ting Ding ,&nbsp;Shi-Lin Du ,&nbsp;Hong-Yi Liang ,&nbsp;Ya-Hui Zhang ,&nbsp;Yong Tao ,&nbsp;Ming-Xiao Li ,&nbsp;Jin Zhang ,&nbsp;Shu-Shen Liu","doi":"10.1016/j.ecoenv.2025.118568","DOIUrl":"10.1016/j.ecoenv.2025.118568","url":null,"abstract":"<div><div>Chemical pollution in real-world environment often involves exposure to combinations of thousands of chemicals. However, due to the vast number of possible combinations, it is nearly impossible to conduct comprehensive mixture toxicity tests and risk assessments for all of them. This study applied frequent itemset mining, a technique traditionally used in market basket analysis, to develop a prevalent mixture system screening (PMSS) method for identifying combinations that frequently co-occur in the environment. PMSS enables efficient data mining of chemical concentrations, allowing for the identification of a small number of prevalent mixture systems from numerous theoretical possibilities. In this study, 16 antibiotics were detected in the Linjiang River and the Xuebu River. Using the PMSS method, 48 prevalent antibiotic combinations (PACs), primarily ranging from binary to septenary combinations, were identified in the Xuebu River and the Linjiang River. The PACs in the surface water presented acceptable ecological risks, whereas the PACs in the sediments exhibited moderate to even high ecological risks. Therefore, targeted risk management measures should be developed for the sediments to reduce the potential harm to benthic organisms. Additionally, a case study demonstrates the application of identified PACs in mixture design. This study provides essential methodological and material support for advancing research on mixture toxicity evaluation and risk assessment.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118568"},"PeriodicalIF":6.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of molecular diagnostic tests for marine mammal DNA viruses, herpesvirus, poxvirus, and anellovirus, using a portable biomeme Franklin thermocycler","authors":"Jieun Park ,&nbsp;Seonghyeon Lee ,&nbsp;Shivani Rajoriya ,&nbsp;Daehyun Kim ,&nbsp;Bohyeon Kim ,&nbsp;Augustine Natasha ,&nbsp;Sara Puspareni Prayitno ,&nbsp;Haryo Seno Pangestu ,&nbsp;Minsung Lee ,&nbsp;Min-Jeong Kim ,&nbsp;Kyunglee Lee ,&nbsp;Seung Won Jung ,&nbsp;Taek-Kyun Lee ,&nbsp;Won-Keun Kim","doi":"10.1016/j.ecoenv.2025.118564","DOIUrl":"10.1016/j.ecoenv.2025.118564","url":null,"abstract":"<div><div>Infectious disease-related mass mortality events (ID-MMEs) in marine mammals have increased dramatically over the past 30 years, and viruses have been identified as the primary cause. This study validates the development and utility of point-of-care diagnostics using handheld devices. Using real-time quantitative polymerase chain reaction (real-time qPCR), we developed and validated a portable diagnostic method for detecting viruses infecting marine mammals in field applications. Specifically, the Franklin portable thermocycler was used to demonstrate the feasibility of field diagnostics for marine mammal viruses. Portable devices enable rapid point-of-care diagnostics and have high mobility to detect viral transmission and variants in resource-limited settings for early response. This device enables rapid and accurate diagnosis of marine mammal viruses in resource-limited environments, facilitating timely intervention to mitigate the spread of infection to marine ecosystems and other species, including humans. Despite these advances, several challenges remain, including the optimization of DNA extraction for point-of-care diagnostics and obtaining clinical samples. In conclusion, this study highlights the potential value of point-of-care diagnostic tests in response to viral diseases in marine mammals.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118564"},"PeriodicalIF":6.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single cell RNA sequencing reveals the reshaping effect of perfluorooctanoic acid on the intestinal microenvironment of mice","authors":"Ya Gao ,&nbsp;Jiayun Guan ,&nbsp;Rui Zhou ,&nbsp;Zewei Li ,&nbsp;Tianyang Chen ,&nbsp;Kaican Yang ,&nbsp;Rong Huang ,&nbsp;Yue Huang ,&nbsp;Siting Zheng ,&nbsp;Zhenhua Huang ,&nbsp;Xiaoxiang Rong","doi":"10.1016/j.ecoenv.2025.118540","DOIUrl":"10.1016/j.ecoenv.2025.118540","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA), a prevalent perfluorinated compound pollutants, has been implicated in the development of various gastrointestinal disorders. However, the specific PFOA-induced intestinal microenvironment alterations remain poorly characterized. In this study, we established a chronic PFOA toxicity model in C57BL/6 mice of both sexes through exposure to PFOA at doses of 0, 1, 5, and 10 mg/kg/day. Single-cell RNA sequencing (scRNA-seq) was performed on the small intestine tissues from female mice, and key findings were validated through flow cytometry and immunohistochemistry. Histopathological analysis revealed that PFOA induced intestinal damage in both sexes, with a more severe impact observed in female mice. scRNA-seq analysis demonstrated that PFOA treatment triggered abnormal proliferation of intestinal epithelial cells, particularly the expansion of Ada^high enterocyte cells, suggesting their potential role in injury repair process. Furthermore, PFOA exposure induced significant remodeling of the intestinal immune microenvironment, characterized by decreased overall lymphocyte infiltration, heightened activation of remaining cytotoxic CD8⁺ T cells, and a notable increase in proliferative CD8⁺ T cells. A significant reduction in FOXP3⁺ regulatory T cells further contributed to a pro-inflammatory shift within the T cell population. Cell-cell interaction analysis revealed enhanced macrophage-CD8⁺ T cell communication via the CCR1-CCL5 axis, suggesting a synergistic role in driving inflammatory responses. This study presents a comprehensive analysis of PFOA-induced intestinal microenvironment remodeling at single-cell resolution, providing novel insights into the pathophysiological mechanisms underlying PFOA-associated intestinal diseases.</div></div><div><h3>Synopsis</h3><div>Minimal research exists on PFOA-induced intestinal microenvironment changes. The study shows that abnormal hyperplasia and dysfunction of intestinal epithelial cell, along with immuno microenvironment dysregulation, create an inflammatory environment, potentially linked to PFOA-induced systemic toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118540"},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new method for internal urinary metabolite exposure and dietary exposure association assessment of 3-MCPD and glycidol and their esters based on machine learning","authors":"Yimei Tian ,&nbsp;Sunan Gao ,&nbsp;Fan Zhang ,&nbsp;Xuzhi Wan ,&nbsp;Wei Jia ,&nbsp;Jingjing Jiao ,&nbsp;Yilei Fan ,&nbsp;Yu Zhang","doi":"10.1016/j.ecoenv.2025.118550","DOIUrl":"10.1016/j.ecoenv.2025.118550","url":null,"abstract":"<div><div>3-Monochloropropane-1,2-diol (3-MCPD) and glycidol along with their esters are commonly found in chemical production, wastewater treatment, food processing, and exhibit toxicity. Accurate exposure assessment is essential for evaluating the environmental hazards and health risks posed by these contaminants. We collected demographic data from 1587 participants and developed seven models using machine-learning algorithms to investigate urinary metabolite exposure and dietary exposure associations of 3-MCPD and glycidol and their esters. Urinary dihydroxypropyl mercapturic acid concentrations, edible oils, and total energy were identified as key predictors of dietary exposure to these contaminants (<em>p</em> &lt; 0.001). The seven machine learning models demonstrated strong predictive capabilities for internal urinary metabolite exposure and dietary exposure associations (average <em>R</em> &gt; 0.6). Among these, generalized additive model and extreme gradient boosting exhibited the strongest correlation and highest accuracy in predicting the associations. We utilized machine learning techniques to link dietary exposure to 3-MCPD, glycidol, and their esters with internal urinary metabolite exposure, providing an innovative and accurate method for risk exposure assessment.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118550"},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small mammal activities structure fine-scale soil microbial communities and coordinate rodenticide effects in the typical steppes of China","authors":"Wei Liu ,&nbsp;Tingbei Bo ,&nbsp;Ning Zhao ,&nbsp;Bin Hu ,&nbsp;Gaojian Li ,&nbsp;Zhishu Xiao","doi":"10.1016/j.ecoenv.2025.118560","DOIUrl":"10.1016/j.ecoenv.2025.118560","url":null,"abstract":"<div><div>Soil microbial community is a diverse combination of microbial species that is influenced by soil moisture, physicochemical properties, biological activities, and other factors. Brandt's vole (<em>Lasiopodomys brandtii</em>) is a small rodent distributed in the typical steppes of China, Mongolia, and Russia, which often causes serious pest to the local areas when its population outbreaks. Yet the impacts of rodenticides on soil microbial diversity and ecosystem functions in Brandt’s vole habitats are not well-known. In this study, we compared the microbial community composition and functional genes between the central and peripheral areas of undisturbed cave communities, as well as grasslands with and without rodenticides. The results showed that the activity of field voles and rodenticides had a significant impact on soil microbial communities, antibiotic resistance genes, and soil element cycling. The use of rodenticides reduces the activity of rodents, reduces pathogenic bacteria in the soil. The use of rodenticides inhibits N cycling, while rodent activity promotes the N cycling. Relative abundance of dissimilatory nitrate reduction nitrate (narGHI or napA), denitrification nitrous oxide (nosZ), nitrogen fixation nitrogen (nifH), denitrification nitric oxide (norB) were significantly decreased in rodenticide soil. The activity of rodents increases the abundance of specific antibiotic resistance genes (ARGs), while rodenticides reduce the abundance of AGRs in the soil. Our study for the first time explores the interaction effects of rodent activity and rodenticide use on soil, providing a reference for understanding the multifunctionality of soil ecosystems and the response of microbial communities to external disturbances (rodents and humans) in the future.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118560"},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oppositely charged proteins lead to different effects on the bioaccumulation kinetics of polystyrene nanoplastics in zebrafish (Danio rerio)","authors":"Guoliang Zhang,&nbsp;Bowen Xiao,&nbsp;Tao Jiang,&nbsp;Tong Zhao,&nbsp;Jinhui Sun,&nbsp;Huamin Liu,&nbsp;Lixin Wang","doi":"10.1016/j.ecoenv.2025.118555","DOIUrl":"10.1016/j.ecoenv.2025.118555","url":null,"abstract":"<div><div>Nanoplastics (NPs) are ubiquitous in the natural aquatic environment, thus there is a major need to understand their potential health risk toward aquatic organisms. Proteins are widely spread in the water environment, they may interact with NPs and significantly impact the ecotoxicology of NPs <em>in vivo</em>. In this study, positively charged lysozyme (LYZ) and negatively charged ovalbumin (OVA) were selected as model proteins, and their effects on the bioaccumulation kinetics of polystyrene nanoplastics (PS-NPs) in zebrafish were investigated quantitatively using a biokinetic model. The adsorption of OVA decreased the uptake rates of PS-NPs, thereby reducing the bioconcentration factors (BCF) of PS-NPs in zebrafish. However, in the presence of LYZ, the uptake rates of PS-NPs increased while the depuration rates decreased significantly, as a consequence, the BCF of PS-NPs in zebrafish were elevated. Dynamic light scattering, transmission electron microscopy, and confocal laser scanning microscopy analysis demonstrated that the adsorption of OVA significantly increased the negative charge and hydrodynamic size of PS-NPs, inhibited the interaction between the nanoparticles and negatively charged cell membrane, thus leading to the alleviated bioaccumulation. In contrast, LYZ changed the surface charge of PS-NPs to positive, enhanced the electrostatic attraction and interaction between PS-NPs and cell membrane, thereby increasing the bioavailability of PS-NPs. Our results highlight the impacts of proteins on the biokinetics of NPs, providing novel insights into the ecological risk of nanoplastics.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118555"},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hollow glass microspheres on the benthic and benthopelagic organisms Capitella sp. and Mytilus edulis","authors":"Julia Farkas ,&nbsp;Stefania Piarulli ,&nbsp;Marianne Molid ,&nbsp;Bjørn Henrik Hansen ,&nbsp;Trond Nordtug ,&nbsp;Tørris Sandsæter ,&nbsp;Margot Ulfsdatter Nyeggen ,&nbsp;Mimmi Throne-Holst ,&nbsp;Andreas Hagemann ,&nbsp;Kaja Cecilie Hellstrøm ,&nbsp;Marianne Aas ,&nbsp;Arne Malzahn","doi":"10.1016/j.ecoenv.2025.118552","DOIUrl":"10.1016/j.ecoenv.2025.118552","url":null,"abstract":"<div><div>Hollow glass microspheres (HGM) are widely used as fillers in composite materials including paints and plastics. Further, due to their high reflectivity, an approach involving HGM application on Arctic sea ice surfaces has been considered as a measure to reduce ice loss and thus help mitigate climate change effects. In this study we investigated potential impacts of HGMs (average size 47 µm) on two marine species, the benthic polychaete <em>Capitella sp.</em> and the filter feeding blue mussel <em>Mytilus edulis</em>. <em>Capitella</em> were exposed to three concentrations of non-buoyant HGMs with and without sediment (1, 10 and 100 mg g⁻¹ sediment equalling 0.1, 1 and 10 g total HGMs). After a 42 day-exposure (juveniles to adult live stages), HGM uptake, survival and growth were determined. Adult <em>Mytilus edulis</em> were exposed for 10 days to three concentrations (1, 10 and 100 mg L⁻¹) of fresh and biofouled HGMs, and HGM uptake, survival, condition index, clearance rate, cytotoxic and genotoxic damage in haemocytes were analysed. Results show that, while both species ingest HGMs, there were no significant impacts on survival. Exposure to medium and high HGM concentrations in presence and absence of sediment significantly impacted growth and development of <em>Capitella</em>. In contrast, the shorter-term exposure to fresh or biofouled HGMs did not affect the nutritional status, condition index or filtering capacity of adult <em>M. edulis</em>. High concentrations of biofouled HGMs did cause a slight response on cellular level in <em>M. edulis</em>, as eosinophilic cell counts decreased. While responses in <em>M. edulis</em> were less clear, the HGM mode of action on <em>Capitella</em> may be related to alterations in energy budget. Further studies are needed to investigate modes of action and determine effect threshold concentrations in potential target species.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118552"},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of ambient air pollution with incident rheumatoid arthritis, subsequent respiratory diseases, and death: A multi-state analysis of a prospective cohort","authors":"Bin-Bin Feng ,&nbsp;Yu-Xuan Zhang ,&nbsp;Xing-Yue Dai ,&nbsp;Mei-Qin Nie ,&nbsp;Yong-Yong Liu ,&nbsp;Ruo-Wei Ma ,&nbsp;Lin Zhu ,&nbsp;Yu-Ye Zuo ,&nbsp;Hai-Feng Pan ,&nbsp;Guo-Cui Wu","doi":"10.1016/j.ecoenv.2025.118557","DOIUrl":"10.1016/j.ecoenv.2025.118557","url":null,"abstract":"<div><h3>Background</h3><div>Previous studies have mostly examined the separate effects of air pollution exposure on the onset of rheumatoid arthritis (RA) or respiratory diseases. However, no studies have further explored the impact of air pollution on the progression from a baseline healthy population to the onset of RA, followed by the further complication of respiratory diseases and, ultimately, mortality.</div></div><div><h3>Methods</h3><div>Based on data from the UK Biobank, we included 340,485 participants without RA or respiratory diseases. Air pollution exposure levels were estimated by land use regression modeling, and multi-state modeling was used to assess the impact of air pollution on RA disease progression trajectories.</div></div><div><h3>Results</h3><div>During a median follow-up of 12.66 years, 3650 participants developed RA, of whom 553 subsequently developed respiratory diseases; 24,016 participants died during this process. We observed that PM_2.5, NO₂, and NO_x exposures adversely affected the development of RA, with PM_2.5 and NO₂ exposures contributing to the subsequent development of respiratory diseases. Furthermore, all four air pollutants were associated with increased mortality risk. Notably, PM_2.5 and NO₂ demonstrated the strongest effects on the transition from RA to death, with hazard ratios (HRs) per interquartile range (IQR) increase of 1.21 (95 % CI: 1.06–1.37) and 1.20 (95 % CI: 1.05–1.37), respectively. However, no significant effect of air pollution was observed on the further progression from respiratory diseases complicating RA to death.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that air pollution abatement may help prevent the onset of RA as well as its further progression.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118557"},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and transcriptomic responses of pumpkin to lead and ozone stress","authors":"Wenjing Liu ,&nbsp;Jiawei Luo ,&nbsp;Donggang Guo ,&nbsp;Quanxi Zhang","doi":"10.1016/j.ecoenv.2025.118567","DOIUrl":"10.1016/j.ecoenv.2025.118567","url":null,"abstract":"<div><div>The co-pollution of ozone (O₃) and lead (Pb) poses significant threats to crop growth and human health, thus attracted serious concern. This study examined the individual and combined effects of Pb and O₃ treatment on the growth, photosynthesis, antioxidant enzyme activity, gene expression, and the ascorbate-glutathione (ASA-GSH) cycle in pumpkin seedlings. The results revealed that Pb and O₃ treatments, both individually and in combination, significantly inhibited the growth of pumpkin seedlings, as evidenced by reductions in leaf area, stem diameter, dry weight, and fresh weight. Remarkably, the combined Pb and O₃ treatment exhibited more pronounced negative effects on plant growth than the individual stressor. This phenomenon could be attributed to two key factors. First, the co-exposure of Pb and O₃ disrupted chlorophyll synthesis and reduced the activity of key photosynthetic enzymes (Rubisco and FBPase) by downregulating the expression of photosynthesis-related genes (RCA, RBCS, and FBPase), thereby impairing photosynthesis and hindering pumpkin growth. Second, the combined treatment reduced antioxidant enzyme (SOD, POD, CAT, and GR) activity, disrupted the ASA-GSH cycle, and triggered the malondialdehyde (MDA) accumulation, thus exacerbating oxidative damage. This study provides new insights into the broader impacts of atmospheric and soil pollution on crop health, resilience and safety.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118567"},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}