Ecotoxicology and Environmental Safety最新文献

{"title":"Physiological and biochemical responses of pepper (Capsicum annuum) to cadmium stress: The mitigating effects of exogenous abscisic acid","authors":"Dehui Tu ,&nbsp;Xiao Tian ,&nbsp;Chuangjing Liang ,&nbsp;Peng Zhou ,&nbsp;Yongping Wang ,&nbsp;Dan Xing ,&nbsp;Yizhang Liu","doi":"10.1016/j.ecoenv.2025.118370","DOIUrl":"10.1016/j.ecoenv.2025.118370","url":null,"abstract":"<div><div>The production of pepper is increasingly challenged by cadmium (Cd) contamination. Abscisic acid (ABA) is supposed to alleviate Cd-induced toxicity and reduce the accumulation of Cd. However, the underlying physiological mechanisms by which ABA regulates Cd tolerance remain unclear. Therefore, this study investigates the mitigation of Cd toxicity in pepper exposed to Cd stress through the application of exogenous ABA under hydroponic conditions. The analysis focused on the subcellular distribution of Cd in the root, its localization in cell wall polysaccharide components, and the associated physiological indices. The results showed that ABA treatment significantly reduced Cd accumulation in pepper roots and shoots, which suggests that ABA effectively decreases Cd translocation from roots to aboveground tissues. This was accompanied by enhanced antioxidant activity, as evidenced by increased peroxidase and catalase activities. Furthermore, ABA regulated the subcellular distribution of Cd in root tissues, decreased Cd content in the cell wall and thereby mitigating Cd toxicity at the cellular level. The addition of ABA also reduced the content of hydrogen peroxide and malondialdehyde, indicating a reduction in lipid peroxidation and cell membrane damage. These findings highlight that ABA can enhance plant tolerance to Cd stress by modifying Cd distribution within the plant, boosting the antioxidant defense mechanism, and altering cell wall composition to limit Cd absorption. This study suggests that exogenous ABA could be a potential strategy for improving Cd resistance in pepper and reducing Cd contamination in crops.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118370"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereoisomeric developmental toxicity of orysastrobin and its isomeric metabolite in zebrafish (Danio rerio) embryos: Cardiac abnormal development by orysastrobin","authors":"Junhee Son ,&nbsp;Chaeeun Kim ,&nbsp;Sung-Eun Lee","doi":"10.1016/j.ecoenv.2025.118391","DOIUrl":"10.1016/j.ecoenv.2025.118391","url":null,"abstract":"<div><div>Orysastrobin (OSB), a strobilurin fungicide, inhibits the normal redox reactions of cytochrome bc1 at the Qo site of target phytopathogenic fungal species. Its frequent detection and high concentrations in natural wetlands and rivers have raised concerns regarding its acute toxicity and potential effects on fish. Therefore, this study examined the acute toxicities of OSB and its stereoisomeric metabolite F001 on zebrafish (<em>Danio rerio</em>) embryos, an animal model. OSB and F001 did not induce substantial mortality or inhibit hatching in the treated embryos. However, OSB exhibited acute toxicities, including pericardial and yolk sac edemas, spine curvature, and bleeding, at 5.0 mg/L concentration. In contrast, F001 did not induce such abnormalities in zebrafish embryos within the tested concentration ranges. Additionally, OSB induced abnormal cardiac development and significantly impaired heart function in embryos, likely due to the up-regulation of key heart development genes, such as <em>kcnn6a</em> and <em>amhc</em>. In OSB-treated embryos, a developmental delay in liver formation was observed. OSB-induced reactive oxygen species in zebrafish embryos. Overall, this study underscores the importance of gathering comprehensive toxicological data on OSB and F001 in fish to fully understand their environmental impacts, emphasizing the urgent need for repeated periodic monitoring to mitigate the environmental risks posed in agricultural waters, reservoirs, and other aquatic ecosystems.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118391"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing identifies cellular heterogeneity in endothelial and epithelial cells associated with nitrogen dioxide-induced acute lung injury","authors":"Weidong Li,&nbsp;Zhenghao Bao,&nbsp;Hongpeng Huang,&nbsp;Yingkai Ma,&nbsp;Yangyang Sun,&nbsp;Xueyang Lin,&nbsp;Weiqiang Sun,&nbsp;Shengran Wang,&nbsp;Ziqi Cui,&nbsp;Chen Yang,&nbsp;Yufeng Yang,&nbsp;Simin Lang,&nbsp;Zheming Yuan,&nbsp;Yongan Wang,&nbsp;Yuan Luo","doi":"10.1016/j.ecoenv.2025.118385","DOIUrl":"10.1016/j.ecoenv.2025.118385","url":null,"abstract":"<div><div>Inhalation of nitrogen dioxide (NO₂), a representative irritant gas, can trigger acute lung injury (ALI), typically characterized by increased permeability and dysfunction of the blood-air barrier. However, the exact mechanisms underlying NO₂ inhalation-induced ALI (NO₂-ALI) remain poorly understood. Using single-cell RNA sequencing (scRNA-seq), we identified significant alterations in endothelial and epithelial cells during NO₂-ALI. Notably, leucine-rich alpha-2-glycoprotein 1 (Lrg1) and uncoupling protein 2 (Ucp2), which have been implicated in ALI progression, were significantly upregulated in endothelial cells following NO₂ exposure (<em>P</em> &lt; 0.05 compared to control). General capillaries (GCs) potentially function as stem cells, facilitating endothelial cell repair and recruiting neutrophils to amplify inflammatory responses. Furthermore, a novel subpopulation of epithelial cells, identified as lymphocyte antigen 6 A⁺ (Ly6a) alveolar cells, showed a significant increase in abundance (<em>P</em> &lt; 0.05 compared to control) and played a pivotal role in alveolar epithelial cell differentiation after NO₂ inhalation. Overall, these findings shed insights into the pathogenic roles of endothelial and epithelial cells in NO₂-ALI.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118385"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing microglial heterogeneity in autophagy impairment of Paraquat-induced Parkinson’s disease-like neurodegeneration","authors":"Yu Zhang ,&nbsp;Yihua Jiang ,&nbsp;Zhen Yu ,&nbsp;Yinhan Li ,&nbsp;Zhiyu Zhang ,&nbsp;Fuli Zheng ,&nbsp;Hong Hu ,&nbsp;Guangxia Yu ,&nbsp;Zhenkun Guo ,&nbsp;Siying Wu ,&nbsp;Wenya Shao ,&nbsp;Huangyuan Li","doi":"10.1016/j.ecoenv.2025.118364","DOIUrl":"10.1016/j.ecoenv.2025.118364","url":null,"abstract":"<div><div>Parkinson's disease (PD) is a prevalent neurodegenerative condition influenced by environmental elements, notably Paraquat (PQ), which is one of the known risk factors. Impaired autophagy is a critical factor in the pathogenesis of PD, yet the cellular heterogeneity related to autophagy in PD has not been thoroughly investigated. Here, we established a PQ-induced PD-like neurodegeneration model and found that PQ impairs autophagy during experimental PD progression. Using single-cell RNA sequencing (scRNA-seq), we elucidated the autophagy-related transcriptomic landscapes in this model, identifying microglia as the central cell type associated with PQ-induced autophagy across all brain cell types. Additionally, microglial subtypes in the PQ-exposed model exhibited significant heterogeneity in gene expression characteristics, biological functions, and roles in autophagic regulation. PQ exposure induced potential genetic transformations between microglial subtypes, which may further disrupt their immune response and energy metabolism regulation functions. Subsequently, we validated the identity transformation of microglia revealed by scRNA-seq in both <em>in vivo</em> and <em>in vitro</em> PQ exposure models. Moreover, we identified a specific microglial subtype primarily responsible for the autophagy-related changes observed in the PQ-exposed model. The expression of the autophagic subtype marker gene Inpp5d may contribute to the regulation of PQ-induced autophagic impairment in BV2 cells. This study generates the first scRNA-seq atlas of autophagy in the context of PQ exposure, highlighting the heterogeneity of microglial subtypes and identifying an autophagy-specific microglial subtype as a central mechanism in the pathology of PQ-induced PD-like neurodegeneration.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118364"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cell wall functions in plant heavy metal response","authors":"Zhuowei Cheng,&nbsp;Chenyu Wang,&nbsp;Fan Tang,&nbsp;Yifeng Zhou,&nbsp;Cheng Zhu,&nbsp;Yanfei Ding","doi":"10.1016/j.ecoenv.2025.118326","DOIUrl":"10.1016/j.ecoenv.2025.118326","url":null,"abstract":"<div><div>Nonessential metals (e.g., cadmium (II), etc) and essential heavy metals (e.g., copper (II), etc) are both toxic for plants at high concentrations. The plant cell wall, which consists of pectin, cellulose, hemicellulose, lignin and proteins, acts as the first barrier for heavy metal ions to enter the cytoplasm. The binding of heavy metal ions within cell wall components is largely determined by the negative charges of functional groups. The cell wall interacts with heavy metal ions through three main mechanisms: ion exchange, chelation models and cell wall remodeling. Various signaling molecules such as nitric oxide and salicylic acid have been implicated in the regulation of cell wall components. An increasing number of reports indicate that microRNAs can target genes related with cell wall synthesis and modification, thereby mediating heavy metal fixation within the cell wall. In this review, we summarize recent advances in understanding the biosynthesis, modifications, and functions of cell wall components under heavy metal stress. We also discuss the interaction mechanisms and the signaling pathways involved in the cell wall-mediated fixation of heavy metals, offering valuable insights into plant heavy metal stress tolerance mechanisms and providing strategic avenues for mitigating heavy metal pollution.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118326"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of dialkylphosphate metabolites and 1- hydroxypyrene in parent-toddler pairs from agricultural communities and their impacts on toddler’s developmental performance","authors":"Boonsita Suwannakul ,&nbsp;Ajchamon Thammachai ,&nbsp;Noppharath Sangkarit ,&nbsp;Surat Hongsibsong ,&nbsp;Ratana Sapbamrer","doi":"10.1016/j.ecoenv.2025.118348","DOIUrl":"10.1016/j.ecoenv.2025.118348","url":null,"abstract":"<div><div>The widespread presence of pesticides and polycyclic aromatic hydrocarbons (PAHs) in agricultural settings raises significant concerns about early-life exposure and its potential impact on toddler health and development. This cross-sectional study evaluated exposure to organophosphate and PAH metabolites among 154 parent-toddler pairs in northern Thailand, focusing on developmental outcomes. Urinary levels of dialkylphosphate (DAP) metabolites and 1-hydroxypyrene (1-OHP) were measured. Results showed that toddlers had significantly higher geometric mean levels of total DAP metabolites with a geometric mean of 24.38 µg/g creatinine compared to 17.25 µg/g creatinine in parents. A significant positive correlation was observed between the urinary levels of total dimethylphosphate (total DMP) and 1-OHP in both parents and toddlers (<em>r</em> = 0.290 for total DMP, <em>r</em> = 0.485 for 1-OHP), indicating shared environmental exposures. Proximity to farmland was a significant factor, with shorter distances associated with higher urinary total DMP and total DAP levels in toddlers (Beta = −19.8, 95 %CI = −37.3, −2.3 for total DMP, and Beta = −27.5, 95 % CI = −47.7, −7.4 for total DAP). Toddlers’ behavior, such as playing on farmland, was linked to significantly elevated urinary 1-OHP levels (Beta = 121.5, 95 % CI = 8.5, 234.5). Notably, an inverse association was found between DMP levels and receptive communication scores (Beta = −0.009, 95 % CI = −0.018, −0.001), suggesting potential adverse effects on neurodevelopment. These findings underscore the vulnerability of toddlers in agricultural communities to environmental contaminants and emphasize the need for targeted interventions to reduce exposure risks and promote safer agricultural practices. The study provides crucial insights for future research and policy development in environmental health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118348"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoremediation of OTC-Cu/Zn contaminated sediment: Synergetic removal mechanism and microbial community response","authors":"Xueqi Zhang ,&nbsp;Shiying Qi ,&nbsp;Jianshi Huang ,&nbsp;Yebin Lu ,&nbsp;Jianfeng Li ,&nbsp;Jun Wei ,&nbsp;Shuiping Cheng","doi":"10.1016/j.ecoenv.2025.118376","DOIUrl":"10.1016/j.ecoenv.2025.118376","url":null,"abstract":"<div><div>Antibiotics and heavy metals (HMs) from aquiculture enter waters and eventually sink into sediments. However, sediments carrying excessive contaminants can become a source of contamination. Macrophytes are frequently employed to treat water contaminated with HMs due to their adaptability, rapid growth rate, and ability to extract contaminants. In this study, oxytetracycline (OTC), and copper (Cu)/zinc (Zn) were chosen to be the target contaminants of antibiotics and HMs in the sediment. A pot experiment was conducted to investigate the phytoremediation effect of two types of macrophytes, <em>Canna indica</em> and <em>Iris pseudacorus</em>. The removal percentages of OTC, Cu, and Zn range from 93.6 % to 97.6 %, 67.4–86.0 %, and 69.8–82.9 % from the sediment, respectively. Adding Cu/Zn facilitated the decomposition of OTC in the sediments, while the presence of OTC transformed Cu/Zn into a more stable form. Both macrophytes extracted HMs mainly through roots accumulation. Higher concentrations of bioavailable forms of Cu/Zn in sediments inhibited plant growth and development, while lower concentrations promoted that. The inhibitory of leaf chlorophyll a+b synthesis by various contaminant treatments showed that OTC&gt;OTC-HMs&gt;HMs and the stimulation of carotenoid synthesis indicated HMs≥OTC-HMs≥OTC. The dehydrogenase (DHA) activities, microbial diversity and abundance in sediment were severely inhibited by HMs, and the inhibition was alleviated by OTC-HMs with <em>C. indica</em>. Proteobacteria, Actinobacteria, and Firmicutes were the dominant bacterial phyla. This study expands the understanding of phytoremediation potential and outlines their physiological and microbial responses to combined antibiotics-HMs.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118376"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-dose polystyrene microplastics exposure increases susceptibility to obesity-induced MASLD via disrupting intestinal barrier integrity and gut microbiota homeostasis","authors":"Gang Wei ,&nbsp;Kai Zhang ,&nbsp;Feng-Jie Shen ,&nbsp;Rong-Rong Xie ,&nbsp;Feng-Wei Wang ,&nbsp;Hua-Qi Guo ,&nbsp;Lin Liu","doi":"10.1016/j.ecoenv.2025.118310","DOIUrl":"10.1016/j.ecoenv.2025.118310","url":null,"abstract":"<div><div>The global incidence of metabolic dysfunction-associated steatotic liver disease (MASLD) has surged in recent years, potentially impacted by both high-energy food intake (e.g., high-fat diet, HFD) and environmental pollutants like microplastics (MPs). However, the combined impacts of MPs exposure and HFD feeding, particularly under long-time exposure, low concentrations MPs conditions, on the MASLD progression remain to be fully elucidated. In this study, C57BL/6 J male mice were fed either a normal chow diet or HFD with or without low-dose MPs (polystyrene) exposure (25–30 μg/kg body weight /day) for 14 weeks. The adverse health effects associated with MASLD development were evaluated, including intestinal permeability, gut microbiota composition, hepatic lipid metabolism, and the mediating role of the gut-liver axis. Additionally, HFD with or without low-dose MPs exposure was withdrawn to further verify this process. Our data demonstrated that low-dose MPs exposure or HFD feeding significantly increased the gut permeability, oxidative stress, pro-inflammatory response and apoptosis, while concurrently contributing to gut dysbiosis (e.g., reduced levels of <em>Akkermansia</em>) and MASLD development. Furthermore, low-dose MPs exposure exacerbated these effects in combination with HFD feeding, exhibiting a ‘double hit’ effect. Notably, the impacts of low-dose MPs exposure combined with HFD feeding on MASLD were difficult to reverse after two weeks withdrawing, likely due to the limited recovery potential of intestinal barrier integrity and gut microbiota homeostasis. These finding underscore the importance of avoiding MPs exposure in the pathogenesis of MASLD, particularly under a metabolic disorder conditions, and provide valuable insights for the developing therapeutic strategies to combat MASLD caused by MPs exposure.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118310"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an interpretable machine learning model for predicting hyperuricemia risk: Based on environmental chemical exposure","authors":"Xiaochuan Lu ,&nbsp;Huawei Kou ,&nbsp;Cong Li ,&nbsp;Runqing Zhan ,&nbsp;Rongrong Guo ,&nbsp;Shengnan Liu ,&nbsp;Peixuan Shen ,&nbsp;Meiyue Shen ,&nbsp;Tingwei Du ,&nbsp;Jiaqi Lu ,&nbsp;Xiaoli Shen","doi":"10.1016/j.ecoenv.2025.118392","DOIUrl":"10.1016/j.ecoenv.2025.118392","url":null,"abstract":"<div><div>Hyperuricemia is a global health concern, with environmental chemicals as risk factors. This study used data of multiple environmental chemical exposures from the 2011–2012 cycle of the National Health and Nutrition Examination Survey (NHANES) to develop an interpretable machine learning model for hyperuricemia risk prediction. The least absolute shrinkage and selection operator (LASSO) regression method was employed to select relevant variables. The dataset was split into training (80 %) and test (20 %) sets and six machine learning models were constructed, including Random Forest (RF), Gaussian Naive Bayes (GNB), Light Gradient Boosting (LGB), Extreme Gradient Boosting (XGB), Adaptive Boosting Classifier (AB), and Support Vector Machine (SVM). Our study identified a hyperuricemia prevalence of 20.58 % in the 2011–2012 NHANES cycle, which was consistent with previous studies. The XGB model exhibited optimal performance, achieving the highest AUC (0.806, 95 % CI: 0.768–0.845), balanced accuracy (0.762; 95 % CI: 0.721–0.802), F1 value (0585; 95 % CI: 0.535–0.635), as well as the lowest Brier score (0.133; 95 % CI:0.122–0.144). Estimated glomerular filtration rate (eGFR), body mass index (BMI), cobalt (Co), mono-(2-ethyl)-hexyl phthalate (MEHP), mono-(3-carboxypropyl) phthalate (MCPP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), 2-hydroxynaphthalene (OHNa2) were identified as the key factors contributing to the predictive model. The results of Shapley additive explanations and partial dependence plots indicated that hyperuricemia was positively associated with MCPP, MEHHP, and OHNa2, while negatively associated with Co and MEHP. This study is the first to predict the risk of hyperuricemia based on multiple environmental chemical exposures using a machine learning model.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118392"},"PeriodicalIF":6.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics and metabolomics reveal the mechanism of cognitive impairment induced by long-term selenium deficiency in mice","authors":"Wanpeng Cheng ,&nbsp;Yani Wang ,&nbsp;Peiyue Sa ,&nbsp;Luyun Liang ,&nbsp;Lantian Zhang ,&nbsp;Guangyan Shen ,&nbsp;Jincheng Luo ,&nbsp;Mengdi Li","doi":"10.1016/j.ecoenv.2025.118367","DOIUrl":"10.1016/j.ecoenv.2025.118367","url":null,"abstract":"<div><div>As the aging population increases, cognitive impairment is emerging as a growing health issue worldwide. Low selenium status has been reported to correlate with cognitive decline in older adults. Nonetheless, the impact of prolonged selenium deficiency on cognitive function in adult mice and the underlying mechanisms remain poorly understood. In this research, male C57BL/6 J mice were given either a normal diet (0.2 mg/kg Se) or a selenium-deficient diet (0.02 mg/kg Se) for 24 weeks to evaluate the impact of long-term selenium insufficiency on their cognitive abilities. We performed hippocampus transcriptome sequencing, real-time PCR, Golgi-Cox staining, transmission electron microscopy, western blotting, and untargeted brain metabolomics to uncover the underlying regulatory mechanism. We found that chronic selenium deficiency impaired the capabilities of object recognition, spatial memory, and self-caring in mice, and disrupted the expression of key genes related to cognitive behavior, dendrite morphogenesis, and synaptic plasticity. Additionally, prolonged selenium deficiency compromised neurite integrity, decreased dendritic spine density, impaired synaptic ultrastructure, and reduced synaptic protein expression. Brain metabolomics revealed that differential metabolites (methylmalonic acid, N-acetyl-1-aspartylglutamic acid, and S-adenosylmethionine) may be involved in the process of cognitive impairment. These findings suggest that perturbation in cognition-related transcriptome profiles, lesions in neurites and synapses, and remodeling of the brain metabolic pattern are involved in the cognitive impairment induced by long-term selenium deficiency. Our study offers a new perspective on the pathogenesis of cognitive impairment, highlighting the critical role of selenium supplementation in maintaining healthy cognitive function.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"299 ","pages":"Article 118367"},"PeriodicalIF":6.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}