Ecotoxicology and Environmental Safety最新文献

{"title":"A novel method for real-time inhalation toxicity assessment in mice using respirometric system: A promising tool for respiratory toxicology","authors":"Yu-Yeong Choi ,&nbsp;Fida Hussain ,&nbsp;So-Yeon Kim ,&nbsp;Ho Jung Bae ,&nbsp;Ju-Yeon An ,&nbsp;Hyun-Jeong Kim ,&nbsp;Ye Eun Cho ,&nbsp;So-Young Cho ,&nbsp;Ji Woong Choi ,&nbsp;Sang-Eun Oh ,&nbsp;Se Jin Park","doi":"10.1016/j.ecoenv.2024.117333","DOIUrl":"10.1016/j.ecoenv.2024.117333","url":null,"abstract":"<div><div>Inhalation toxicity assessment is a crucial tool for the identification and classification of hazardous materials like volatile organic carbons, aerosols, and particulate matter. Unlike traditional acute inhalation toxicity studies that use mortality as an endpoint, the Fixed Concentration Procedure (FCP) emphasizes \"evident toxicity\" by monitoring behavior, weight, and food intake. This reduces reliance on mortality but doesn't directly address respiratory system impact. The present study introduced a respirometer-based inhalation toxicity and respiratory status assessment method. The toxicity evaluation system integrated a respirometric system with an animal exposure chamber, enabling real-time monitoring of oxygen consumption. The ICR mice were exposed to various concentrations of benzene (10, 20, 40, and 80 mg/L of air), toluene (7.5, 15, 30, and 60 mg/L of air), and xylene (7.5, 15, 30, and 60 mg/L of air). The respiration rate decreased by 70 % and 69 % for benzene (80 mg/L of air) and toluene (60 mg/L of air), respectively, with EC₅₀ values of 32.5 mg/l and 21.2 mg/L based on oxygen consumption. Xylene did not exhibit EC₅₀ values at the tested concentrations. However, the oxygen consumption rate significantly decreased (46 %) at high concentrations (60 mg/L of air), indicating sub-lethal toxicological effects. Furthermore, the present study was also validated in the bleomycin-induced idiopathic pulmonary fibrosis (IPF) model, demonstrating its reliability as a respiratory impairment marker. The results exhibited a strong correlation between weight loss and less oxygen consumption in the BLM group (bleomycin-induced) as compared to the SHAM group (control), which was confirmed by histological examination and protein marker analysis. The results suggest the potential use of oxygen consumption as an endpoint measurement in inhalation toxicity assessment tests without animal sacrifice, and the present study could be useful for providing valuable insights into disease progression and pharmacological interventions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117333"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to perfluorodecanoic acid impairs follicular development via inducing granulosa cell necroptosis","authors":"Zekun Liu ,&nbsp;Zhenyan Cui ,&nbsp;Chunming Li ,&nbsp;Kean Lu ,&nbsp;Kelie Chen ,&nbsp;Wei Cui ,&nbsp;Yihua Wu ,&nbsp;Dajing Xia","doi":"10.1016/j.ecoenv.2024.117268","DOIUrl":"10.1016/j.ecoenv.2024.117268","url":null,"abstract":"<div><div>Per- and polyfluoroalkyl substances (PFAS) have attracted significant attention due to their environmental toxicity. However, the detrimental impact of PFAS on the development of the female reproductive system remains controversial. In this study, we investigated the effects of three specific PFAS compounds perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA) on ovarian development. Among these compounds, PFDA demonstrated the most pronounced cytotoxic effect on ovarian granulosa cells. The results showed that a 200 μM concentration of PFDA induced cell apoptosis via the intrinsic pathway by elevating reactive oxygen species (ROS) levels and activating Caspase-9 and Caspase-3. Furthermore, 200 μM PFDA triggered necroptosis, a form of regulated cell death (RCD), through the receptor-interacting serine/threonine kinase 1 (RIPK1), receptor interacting protein kinase 3 (RIPK3), and mixed-lineage kinase domain-like protein (MLKL) axis, mediated by inhibition of the canonical apoptosis proteolytic enzyme Caspase-8. In vivo experiments confirmed that mice exposed to PFDA displayed a significantly reduced ovarian index compared to the control group, accompanied by evident follicular atresia. Ovarian tissues from the PFDA-exposed group showed upregulated necroptosis markers, which were effectively mitigated by inhibiting the phosphorylation of RIPK1 at Ser166. Importantly, this study provides the first evidence that PFDA disrupts ovarian development through a novel mechanism involving the RIPK1-mediated necroptosis pathway, alongside the detection of the intrinsic apoptosis pathway. This greatly expands our insight into the effects of PFDA on cell death. This finding highlights the potential public health hazards associated with PFDA exposure and emphasizes the need for further research to fully understand its broader implications.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117268"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupled bio-solar photocatalytic treatment for reclamation of water polluted with pharmaceutical and pesticide residues: Impact on tomato irrigation","authors":"Marina Aliste ,&nbsp;Virginia Hernández ,&nbsp;Abderrazak El Aatik ,&nbsp;Gabriel Pérez-Lucas ,&nbsp;José Fenoll ,&nbsp;Simón Navarro","doi":"10.1016/j.ecoenv.2024.117291","DOIUrl":"10.1016/j.ecoenv.2024.117291","url":null,"abstract":"<div><div>Reusing reclaimed water for crop irrigation can mitigate water scarcity in agriculture; however, contaminants such as pharmaceuticals and pesticides in wastewater pose risks. This study investigated the impact of a coupled bio-solar photocatalytic treatment on the reclamation of water polluted with seven pharmaceuticals and seven pesticides for irrigation of two tomato crop cycles. Pollutant residues were removed using natural sunlight and TiO₂/Na₂S₂O₈ in a pilot plant located in Murcia, Spain. Efficient removal (&gt; 96 %) of all target pollutants was achieved in the effluent after coupled treatment. Reclaimed water was then used to irrigate the tomato crops, and several yield and quality parameters were analysed to evaluate the effects on the harvested tomatoes. No significant differences were observed in the total yield, number and mean fruit weight, size, pericarp firmness, external colour, and nutritional data between the crops irrigated with reclaimed, control, and polluted water. However, differences in the degree of ripeness were observed. None of the investigated pollutants was detected above the limit of quantification in tomato samples irrigated with reclaimed water, except for venlafaxine (0.028 µg kg⁻¹) in the second crop cycle. When the crop was irrigated with polluted water, different pollutant residues were detected in soil (10) and tomato (4) samples. The results suggest that coupled bio-solar photocatalytic treatment is an effective method for reclaiming water polluted with pharmaceutical and pesticide residues, and the reclaimed water can be safely used for tomato irrigation without compromising crop yield and quality.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117291"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-204 regulates autophagy and cell viability by targeting BDNF and inhibiting the NTRK2-dependent PI3K/Akt/mTOR pathway in a human granulosa cell line exposed to bisphenol A","authors":"Chunming Li ,&nbsp;Zhenyan Cui ,&nbsp;Zekun Liu ,&nbsp;Huiyu Fan ,&nbsp;Yibing Lan ,&nbsp;Jie Luo ,&nbsp;Fei Ruan ,&nbsp;Yizhou Huang ,&nbsp;Ketan Chu ,&nbsp;Yihua Wu ,&nbsp;Dajing Xia ,&nbsp;Jianhong Zhou","doi":"10.1016/j.ecoenv.2024.117304","DOIUrl":"10.1016/j.ecoenv.2024.117304","url":null,"abstract":"<div><div>Bisphenol A (BPA) is a widespread endocrine disruptor that mimics estrogen. The accumulation of BPA within the human body has been shown to be detrimental to ovarian function. However, few studies have focused on the specific mechanisms by which it causes harm to granulosa cells (GCs), pivotal ovarian cells that are responsible for the growth and function of oocytes. In vitro research was conducted using human GC lines (KGN cells). The cells were exposed to various concentrations of BPA (0.1, 1, 10, or 100 µM) for either 24 or 48 hours. Here, our findings indicate that 100 μM BPA inhibits KGN cell proliferation and promotes cell autophagy through inhibiting the PI3K/Akt/mTOR pathway. Interestingly, these effects could be partly reversed by an NTRK2 activator (LM22b-10). NTRK2 is the receptor for BDNF. Moreover, via the use of bioinformatics tools, miR-204 was predicted to target BDNF. Additionally, our findings confirmed that miR-204 has the ability to directly target BDNF through a luciferase assay. Downregulation of miR-204 abrogated the BPA exposure-mediated effects on proliferation and autophagy. Furthermore, the inhibition of miR-204 significantly reversed the downregulation of PI3K/Akt/mTOR pathway-related molecules. Similarly, we validated miR-204 as a novel miRNA involved in BPA-mediated damage to GC proliferation and autophagy, and our data provide the first in vitro evidence that increasing miR-204 expression and inhibiting the BDNF/NTRK2-mediated PI3K/Akt/mTOR signaling pathway are involved in the BPA-induced toxic effects in KGN cells.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117304"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide residues: Bridging the gap between environmental exposure and chronic disease through omics","authors":"Lingling Cao ,&nbsp;Qiyuan Kang ,&nbsp;Yuan Tian","doi":"10.1016/j.ecoenv.2024.117335","DOIUrl":"10.1016/j.ecoenv.2024.117335","url":null,"abstract":"<div><div>Pesticide residues, resulting from agricultural practices, pose significant health and environmental risks. This review synthesizes the current understanding of pesticide impacts on the immune system, highlighting their role in chronic diseases such as asthma, diabetes, Parkinson's disease (PD) and cancer. We emphasize the significant role of omics technologies in the study of pesticide toxicity mechanisms. The integration of genomics, proteomics, metabolomics, and epigenomics offers a multidimensional strategy for a comprehensive assessment of pesticide effects, facilitating personalized risk management and policy formulation. We advocate for stringent regulatory policies, public education, and global cooperation to enhance food safety and environmental sustainability. By adopting a unified approach, we aim to mitigate the risks of pesticide residues, ensuring human health and ecological balance are preserved.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117335"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PMMA nanoplastics induce gastric epithelial cellular senescence and cGAS-STING-mediated inflammation via ROS overproduction and NHEJ suppression","authors":"Xiao Li ,&nbsp;Yixing Huang ,&nbsp;Dan Zu ,&nbsp;Haidong Liu ,&nbsp;Hanyi He ,&nbsp;Qimei Bao ,&nbsp;Yanhua He ,&nbsp;Chen Liang ,&nbsp;Guoyan Luo ,&nbsp;Yaoshu Teng ,&nbsp;Yin Shi ,&nbsp;Zu Ye ,&nbsp;Xiangdong Cheng","doi":"10.1016/j.ecoenv.2024.117284","DOIUrl":"10.1016/j.ecoenv.2024.117284","url":null,"abstract":"<div><div>The increasing environmental presence of nanoplastics (NPs) has raised concerns about their potential impact on biological systems. We investigated the repercussions of polymethyl methacrylate (PMMA) NPs exposure on normal gastric epithelial cells and revealed a pronounced increase in senescence-associated β-galactosidase activity and G1 phase cell cycle arrest. Our study demonstrated a dose-dependent increase in reactive oxygen species (ROS) and DNA damage, underscoring the pivotal role of ROS in PMMA NPs-mediated effects, a novel contribution to the existing body of knowledge dominated by polystyrene particles. Furthermore, we explored the influence of PMMA NPs on DNA damage response mechanisms, highlighting the significant inhibition of nonhomologous end-joining (NHEJ). Our findings help to elucidate the consequent genomic instability, as evidenced by increased chromosomal aberrations and micronuclei formation. By connecting these cellular manifestations to organism-level effects, we hypothesize that PMMA NPs play a critical role in aging processes. Our work revealed an activated cGAS-STING signaling pathway after PMMA NPs exposure, which correlated with aging-related inflammation and behavioral changes in mice. Importantly, our study provides comprehensive evidence of PMMA NPs-induced premature aging in gastric epithelial cells, shedding light on the molecular intricacies underlying DNA damage, repair impairment, and inflammation. Our research prompts heightened caution regarding the risks of NPs exposure and calls for further investigation into the broader implications of these environmental pollutants on aging processes in higher organisms.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117284"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juvenile hormone III improves honeybee resistance to imidacloprid by protecting the midgut","authors":"Nana Wang ,&nbsp;Kunrong Wang ,&nbsp;Li Lei ,&nbsp;Longtao Zhang ,&nbsp;Xingqi Guo ,&nbsp;Baohua Xu ,&nbsp;Ying Wang ,&nbsp;Chen Wang","doi":"10.1016/j.ecoenv.2024.117280","DOIUrl":"10.1016/j.ecoenv.2024.117280","url":null,"abstract":"<div><div>Imidacloprid is one of the most commonly used insecticides and seriously threatens the survival of honeybees. Juvenile hormone III (JHIII), an important hormone in honeybees, plays a central role in the life cycle of honeybees; however, the mechanism by which JHIII responds to imidacloprid stress has not been determined. In this study, the resistance of <em>Apis cerana cerana</em> (<em>Acc</em>) to imidacloprid was greater than that of <em>Apis mellifera</em> (<em>Am</em>), and the application of JHIII significantly increased the resistance of both species of honeybees. RT<img>qPCR revealed that two genes, <em>juvenile hormone acid methyltransferase</em> (<em>JHAMT</em>) and <em>methyl farnesoate epoxidase</em> (<em>MFE</em>), which are involved in JHIII biosynthesis, are key genes for improving resistance to imidacloprid via JHIII in honeybees. Silencing <em>JHAMT</em> and <em>MFE</em> with RNAi resulted in a lower content of JHIII in <em>Acc</em> and reduced resistance to imidacloprid. The exogenous application of JHIII compensated for the reduced resistance phenotype caused by gene silencing. Furthermore, histological examination of paraffin sections revealed that imidacloprid treatment caused loose intestinal cell arrangement, slight damage to columnar digestive cells and muscle cells, and chromatin condensation in basal layer cells. JHIII treatment reduced the degree of damage to the honeybee midgut cells and improved resistance to imidacloprid. The results of this study provide a new perspective and molecular basis for the study of the regulation of JHIII and the toxicity of imidacloprid in honeybees.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117280"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of growth performance and characterization of pathological lesions in long-term ammonia-exposed pigs","authors":"Byungkwan Oh ,&nbsp;Junsu Park ,&nbsp;Eunju Kim ,&nbsp;Siyoung Seo ,&nbsp;Bumseok Kim ,&nbsp;Sang-Ik Oh","doi":"10.1016/j.ecoenv.2024.117318","DOIUrl":"10.1016/j.ecoenv.2024.117318","url":null,"abstract":"<div><div>Ammonia (NH₃) is a major cause of odor emissions from swine farms, and exposure to high concentrations of NH₃ in short-term periods has been reported to cause respiratory and systemic disorders in pigs. However, the impact of long-term NH₃ exposure on pig health and productivity remain unknown. This study aimed to assess the impact of long-term NH₃ exposure on growth performance and pathological outcomes in pigs. Pigs were reared in the treatment room [34.8 mg/m³ NH₃ concentration (50 ppm); TRT group, <em>n</em> = 40] and the control room [5.6 mg/m³ (8 ppm); CON, <em>n</em> = 40]. The experimental period was 107 days (from weaning piglets to market age). Our findings revealed that long-term NH₃ exposure results in severe respiratory and systemic pathological lesions, including chronic tracheitis, epithelial metaplasia of the trachea, severe interstitial pneumonia, myocarditis, and interstitial nephritis. In addition, the TRT group exhibited reduced productivity, with two deaths, indicating that uncontrolled NH₃ concentrations on pig farms could be linked to a decline in growth performance and even death. Transcriptomic analysis revealed significant changes in immune and inflammatory pathways in lung from TRT pigs, including dysregulated cytokine signaling and incomplete inflammatory responses. The enrichment of DEGs in pathways, such as Cytokine-cytokine receptor interaction, JAK-STAT, and Toll-like receptor signaling, indicated chronic NH₃ exposure disrupted immune homeostasis, contributing to chronic inflammation and impaired tissue repair. In conclusion, this study provides valuable insights into the detrimental effects of NH₃ exposure on pig health and productivity under farm conditions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117318"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambient air pollution and hospital admission for interstitial lung diseases: A multicenter hospital-based case-crossover study","authors":"Yi Zheng ,&nbsp;Jun Liu ,&nbsp;Nongping Feng ,&nbsp;Jing Wei ,&nbsp;Xiaohong Jia ,&nbsp;Lu Luo ,&nbsp;Ruijun Xu ,&nbsp;Chunxiang Shi ,&nbsp;Rui Wang ,&nbsp;Hong Sun ,&nbsp;Yuewei Liu","doi":"10.1016/j.ecoenv.2024.117289","DOIUrl":"10.1016/j.ecoenv.2024.117289","url":null,"abstract":"<div><div>Interstitial lung diseases (ILDs) lead to increased morbidity and premature deaths, imposing a significant burden on public health worldwide. Recently, several studies have linked ambient air pollution with the acute exacerbation of certain ILDs, but the evidence remains limited and inconclusive. With a multicenter hospital-based case-crossover design, we investigated 9128 patients who resided in Jiangsu province, China, and were admitted for ILDs between 2019 and 2022. Residential exposure to particulate matter with an aerodynamic diameter ≤ 2.5 µm (PM_2.5), PM₁₀, sulfur dioxide (SO₂), nitrogen dioxide (NO₂), carbon monoxide (CO), and ozone (O₃) was assessed using our validated grid datasets. We fitted conditional logistic regression models to examine associations of exposure to air pollutants with ILD admission. A 10 µg/m³ increment of exposure to SO₂ and NO₂ was positively associated with a 16.18 % (95 % confidence interval [CI]: 3.79 %, 30.03 %) and 4.06 % (0.75 %, 7.49 %) increase in odds of ILD admission, respectively. All these associations appeared to be linear and the association of SO₂ exposure was significantly stronger among older adults. We estimated that over 10 % of ILD admissions could be attributable to exposure to SO₂ and NO₂. This study provides compelling evidence on the association of exposure to ambient air pollutants (including SO₂ and NO₂) with an increased odds of ILD hospitalizations. Our findings indicate that SO₂ and NO₂ exposures can lead to the exacerbation of ILDs, especially in elderly, and that the disease burden is considerable.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117289"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emamectin benzoate-induced stress significantly affects the gut microbiome of adult Zeugodacus cucurbitae","authors":"Ruwen Li ,&nbsp;Zhenya Tian ,&nbsp;Jingfang Yang ,&nbsp;Xuyuan Gao ,&nbsp;Hongsong Chen ,&nbsp;Yusha Wang ,&nbsp;Zhongshi Zhou","doi":"10.1016/j.ecoenv.2024.117312","DOIUrl":"10.1016/j.ecoenv.2024.117312","url":null,"abstract":"<div><div>The detoxification mechanisms in insects, which are triggered by insecticides, alter the diversity of their intestinal microorganisms. Emamectin benzoate is an insecticide used to control <em>Zeugodacus cucurbitae</em> (Coquillett), a globally significant pest. In this study, high-throughput sequencing, traditional isolation and culture methods, and single bacterial 16S rDNA sequencing were used to analyze the diversity and functional predictions of intestinal microbial communities in <em>Z. cucurbitae</em> adults exposed to emamectin benzoate. The results showed that the intestinal microorganisms of <em>Z. cucurbitae</em> on <em>Cucumis sativus</em> and <em>Benincasa hispida</em> var. chieh-qua were primarily composed of the phyla Proteobacteria and Bacteroidetes and genera <em>Providencia</em>, <em>Enterobacter</em>, <em>Citrobacter,</em> and <em>Klebsiella</em>. The relative abundances of <em>Citrobacter</em>, <em>Enterobacter</em>, <em>Klebsiella,</em> and <em>Raoultella</em> decreased with the induced stress, whereas those of <em>Providencia</em> and <em>Pectobacterium</em> increased. Diversity analysis revealed significant differences in the midgut flora of <em>Z. cucurbitae</em> before and after stress induction with emamectin benzoate.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117312"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}