Xianfei Huang, Bin Lu, Huijuan Liu, Xianliang Wu, Yang Liu
{"title":"Ecotoxicological impacts of microplastics to gut microbiota: Response mechanism, challenges and environmental sustainability-A review.","authors":"Xianfei Huang, Bin Lu, Huijuan Liu, Xianliang Wu, Yang Liu","doi":"10.1016/j.ecoenv.2025.118748","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2025.118748","url":null,"abstract":"<p><p>Microplastics (MPs) entering the gastrointestinal tract of hosts through various pathways alter the community structure and metabolic activity of the gut microbiota, thereby affecting the normal physiological functions of the host. However, there is still huge knowledge gap in the effects of MPs on gut microbiota of organisms. This review mainly summarized comprehensively the response mechanism of gut microbiota of three typical organisms, including aquatic animal (fish), rodent (mouse), and soil animal (earthworm) after MPs entering their gastrointestinal tract. MPs pose substantial health risks to organisms, supported by increasing evidence from animal and human studies. MPs can impair the digestive systems of fish, mice, and earthworms, leading to reduce appetite, slow growth, and diminish reproductive capacity. Additionally, MPs can damage the intestinal mucosal barrier, further compromising health. MPs can increase the absorption of fat, and the lipase on the surface of lipid coated MPs can digest triglyceride, which maybe a potential mechanism leading to changes in fat metabolism. Meanwhile, this review also briefly elaborated a key step and important model for evaluating MPs using gut microbiota as the target organ. Ultimately, this review summarized the limitations in gut microbiota studies of MPs and their future development directions to better comprehend the effects of MPs on the animals and human gut microbiota.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118748"},"PeriodicalIF":6.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Senuri Wijekoon , Yeinner Tarazona , Mike Hightower , Huiyao Wang , Pei Xu , Yanyan Zhang
{"title":"Comprehensive cytotoxicity assessment of treated produced water from thermal distillation using human cell lines","authors":"Senuri Wijekoon , Yeinner Tarazona , Mike Hightower , Huiyao Wang , Pei Xu , Yanyan Zhang","doi":"10.1016/j.ecoenv.2025.118726","DOIUrl":"10.1016/j.ecoenv.2025.118726","url":null,"abstract":"<div><div>Produced water (PW) could be an alternative water resource after treatment for fit-for-purpose applications. However, comprehensive studies assessing the impact of treated PW exposure on human health are still lacking. Herein, we evaluated the effect of untreated and treated PW from the Permian Basin after two thermal distillation systems followed by granular activated carbon (GAC) and zeolite on human cell lines, including intestinal epithelial (Caco-2) cells, breast cancer cells (MCF-7), and embryonic kidney (HEK293) cells. Cells were exposed to untreated PW, thermal distillate, and post-treated PW after GAC and zeolite treatment. Various <em>in vitro</em> toxicity assays were conducted to assess cell viability, necrosis, apoptosis, oxidative stress, estrogenicity, and aryl hydrocarbon receptor (AhR) activation after PW exposure. Overall, untreated PW caused significant cytotoxicity, reduced cell viability, induced oxidative stress, and apoptosis, whereas distillate and GAC+zeolite-treated PW did not induce significant toxicity. As shown by E-screen assays, significant estrogenic activity was observed in feed PW and the distillate but not in the post-treated PW. CYP1A1 gene upregulation was observed in the distillate, suggesting activation of AhR by residual organic compounds. Post-treated PW did not induce AhR activation, highlighting the need for post-treatment following thermal distillation to mitigate residual xenobiotic organic compounds. Given that thermal distillation followed by GAC and zeolite filtration eliminated all adverse impacts on human cell lines, this integrated treatment process demonstrates strong potential for safe discharge and beneficial reuse of treated PW.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118726"},"PeriodicalIF":6.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yeonghoon Son , Ye Ji Jeong , Hyun-Yong Kim , Soo-Ho Lee , Yoonsoo Choi , Hyung-Do Choi , Hae-June Lee
{"title":"Behavioral and neurochemical changes in mice induced by low-level lead exposure: Implications for ADHD and conduct disorders","authors":"Yeonghoon Son , Ye Ji Jeong , Hyun-Yong Kim , Soo-Ho Lee , Yoonsoo Choi , Hyung-Do Choi , Hae-June Lee","doi":"10.1016/j.ecoenv.2025.118735","DOIUrl":"10.1016/j.ecoenv.2025.118735","url":null,"abstract":"<div><div>Lead (Pb) exposure in children and adolescents poses a significant public health risk due to its potential neurotoxic effects. While high-level Pb exposure is known to impair learning and cognition, the behavioral and molecular consequences of low-level Pb exposure during developmental periods remain poorly understood. This study examined behavioral and neurochemical changes in mice exposed to Pb acetate exposure via drinking water from 4 to 8 weeks of age, corresponding to the juvenile through early adult developmental stages in mice. Mice exposed to 30 mg/L resulting in blood lead levels (BLLs) of 1.26 ± 0.089 µg/dL, while the 300 mg/L group, included as a neurotoxic reference, displayed BLLs exceeding 10 μg/dL. Behaviorally, exposure to 30 mg/L Pb did not affect locomotor activity, however, mice exposed to 300 mg/L exhibited hyperactivity and impaired nesting. Notably, impulsive and compulsive behaviors were significantly altered even at 30 mg/L. Neurochemically, qPCR and Western blotting analysis revealed downregulation of DOPA decarboxylase (Ddc), an enzyme crucial for dopamine synthesis, alongside decreased dopamine levels in the striatum. Pb exposure also disrupted striatal dopaminergic signals, including tyrosine hydroxylase (TH) and dopamine D2 receptors (D2R). These findings suggest that even low-level Pb exposure can lead to behavioral dysfunctions by disrupting striatal dopaminergic signaling, highlighting a previously underexplored mechanism of Pb neurotoxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118735"},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sustainable solution for microplastic removal: Sequential biodegradation and detoxification of polyethylene terephthalate microplastics by two natural microbial consortia.","authors":"Minoo Giyahchi, Hamid Moghimi","doi":"10.1016/j.ecoenv.2025.118738","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2025.118738","url":null,"abstract":"<p><p>Polyethylene terephthalate (PET) is a widely used plastic polymer, and its microplastics pose significant threats to ecosystems. One promising approach to addressing this issue is biodegradation using microbial consortia. This study implemented a two-stage biodegradation strategy using microbial consortia to degrade PET microplastics and detoxify their by-products. In the first stage, a bacterial/fungal consortium dominated by Ralstonia, Bradyrhizobium, Exophiala, and Vanrija achieved a 28 ± 2 % degradation efficiency over 60 days, converting PET into medium-chain alkanes (as confirmed by GC-MS analysis), with a maximum CO<sub>2</sub> evolution rate of 722 ppm. Physical and chemical analyses, including Scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, and Fourier transform infrared (FTIR) spectroscopy, revealed structural destruction, mesopore formation, and ester bond breakage of the microplastics. Toxicity assessment of by-products showed a 40 % reduction in human endothelial cell viability, necessitating further detoxification. The second stage utilized a bacterial consortium dominated by Ochrobacterium and Achromobacter, which effectively reduced toxic by-products to 20 %. This study emphasizes the dual focus on efficient PET degradation and the safe decomposition of harmful by-products, showcasing the potential of sequential biodegradation strategies as sustainable solutions for microplastic pollution.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118738"},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edita Paulikaitė-Bivainė , Laurynas Orla , Justina Alčauskaitė , Evita Sėrikovaitė , Rokas Zalūba , Vaidotas Valskys , Valdas Šimčikas , Rokas Buišas , Rasa Aukštikalnienė , Violeta Žalgevičienė , Grita Skujienė
{"title":"Behavioural and cyto-genotoxic effects in adult rats and induced congenital anomalies to their embryos, exposed to environmentally relevant concentrations of phthalates","authors":"Edita Paulikaitė-Bivainė , Laurynas Orla , Justina Alčauskaitė , Evita Sėrikovaitė , Rokas Zalūba , Vaidotas Valskys , Valdas Šimčikas , Rokas Buišas , Rasa Aukštikalnienė , Violeta Žalgevičienė , Grita Skujienė","doi":"10.1016/j.ecoenv.2025.118736","DOIUrl":"10.1016/j.ecoenv.2025.118736","url":null,"abstract":"<div><div>Phthalates are endocrine-disrupting chemicals widely used in everyday plastics and are increasingly recognised as dangerous to humans and ecosystems. In Lithuania, ineffective wastewater treatment has led to rising contamination with phthalates, particularly di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP). As most prior studies used high short-term doses, this study focused on the effects of daily consumption of low environmentally relevant concentrations of DEHP and DBP on female rat physiology, reproduction, embryotoxicity and cyto-genotoxicity. Behavioural observations were included as an exploratory component to assess potential effects. Rats were given environmentally relevant phthalates with food for two months in three groups (200 µg/kg of DEHP, 100 µg/kg of DBP and a mix of both) and two groups for positive control (1000 µg/kg of DEHP and 500 µg/kg of DBP). The negative control group did not receive phthalates. Standard behavioural tests were conducted twice, general blood tests were carried out, and the rats were mated. On the 21st day of gestation, the pregnant rats were euthanised, bone marrow samples were taken for the micronucleus assay, and embryo viability and congenital anomalies were recorded. No significant behavioural or physiological changes were observed in adult rats. However, phthalates increased micronuclei in polychromatic erythrocytes and reduced the PCE/NCE ratio, indicating cyto-genotoxic effects. Long-term exposure to DEHP and DBP at environmentally relevant concentrations induced embryotoxic effects: embryo viability was compromised, with resorptions and morphological abnormalities. In summary, our results suggest that female exposure to phthalates, even in low environmental doses, has hidden cyto-genotoxic, reprotoxic and embryotoxic effects.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118736"},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Combination of solid-liquid hollow fiber liquid phase microextraction method with electromembrane extraction for extraction of Tolmetin and its determination followed by high performance liquid chromatography-ultraviolet detection.","authors":"Samaneh Niazipour, Jahan Bakhsh Raoof, Milad Ghani, Reza Ojani","doi":"10.1016/j.ecoenv.2025.118732","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2025.118732","url":null,"abstract":"<p><strong>Objective: </strong>Herein, a reliable and sensitive method for the determination of tolmetin in biological samples was developed by combining solid-liquid hollow fiber liquid phase microextraction (HF-LPME) method with electromembrane extraction (EME) followed by high-performance liquid chromatography with UV detection (HPLC-UV), to enable safe and effective therapeutic monitoring.</p><p><strong>Methods: </strong>The proposed method performance was significantly enhanced through incorporating of MOF-199, synthesized via the solvothermal method and characterized by FE-SEM, XRD, FT-IR and BET analyses. Optimization of key extraction parameters was initially performed using the one-at-a-time method, while other parameters were systematically optimized through experimental design to achieve high sensitivity and reproducibility.</p><p><strong>Results: </strong>Under the optimal condition (1-octanol as supported liquid membrane (SLM) solvent, 40 V as applied voltage, donor phase pH of 9.0, acceptor phase pH of 12.7, stirring rate of 400 rpm, extraction time of 5 min and MOF-199 loading of 4 mg mL<sup>-1</sup>) the method yielded linear dynamic ranges of 1-200 µg L<sup>-1</sup> (water and urine matrices) and 2-100 µg L<sup>-1</sup> (plasma matrix), with correlation coefficients (r²) above 0.9926. The limits of detection were 0.34, 0.38 and 0.41 µg L<sup>-1</sup> in water, urine, and plasma, respectively. The method showed relative recovery rates between 90 % and 100 % in real plasma and urine samples. Additionally, the method attained a Blue Applicability Grade Index (BAGI) score of 67.5, confirming its high applicability and resource-efficient design.</p><p><strong>Conclusion: </strong>This approach offers high sensitivity, short extraction time, and excellent selectivity and reproducibility, making it well suited for trace analysis of tolmetin in complex matrices.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118732"},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elisabeth Krieger , Florence Fischer , Julia Howanski , Marita Wagner , Federica Romanelli , Beate Fink , Mario Bauer , Anne Schumacher , Tobias Kretschmer , Ana C. Zenclussen
{"title":"Implications of a combined perinatal exposure to BPA and BP-3 for offspring folliculogenesis and ovarian function in mice","authors":"Elisabeth Krieger , Florence Fischer , Julia Howanski , Marita Wagner , Federica Romanelli , Beate Fink , Mario Bauer , Anne Schumacher , Tobias Kretschmer , Ana C. Zenclussen","doi":"10.1016/j.ecoenv.2025.118750","DOIUrl":"10.1016/j.ecoenv.2025.118750","url":null,"abstract":"<div><div>Endocrine disrupting chemicals (EDCs), like bisphenol A (BPA) and benzophenone-3 (BP-3), can interfere with hormone systems, posing risks to fertility and reproduction. Exposure to EDCs is unavoidable making it a relevant environmental health topic, however the impact of real-life EDC mixtures is largely unknown. This study explored the effects of a combined BPA and BP-3 exposure at tolerable intake levels for humans during pregnancy and early life on ovarian development and function in an established mouse model. Mice were daily exposed to concentrations of 4 µg/kg BPA orally, 50 mg/kg BP-3 dermally, and the combination of BPA+BP-3 through gestation and lactation, a susceptible developmental period. Female offspring of BPA and BP-3 exposed mice exhibited increased birth weight and elevated bodyweight by postnatal day 7. By day 30, after hormonal stimulation to induce ovulation, exposed offspring showed disrupted ovarian follicle maturation and altered ovarian response to stimulation with exogenous gonadotropins. Moreover, the number of NK cells rose in the ovaries, and genes linked to hormone signaling, hormone synthesis, and ovarian tissue remodeling were altered relative to unexposed controls. These findings suggest that early life exposure to BPA and BP-3 at environmentally relevant doses impairs ovarian development and function in mice indicating that immune cells and hormonal signaling in the ovaries are targets of endocrine disruptors at relevant concentrations. Such endocrine disruption may be compromising fertility and reproductive health in later life. Our research underscores the importance of investigating the impact of combined EDC exposure on the reproductive system.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118750"},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tolerance mechanisms of two species of Indocalamus under soil Pb stress and the effects of rhizosphere environmental factors on tolerance","authors":"Yedan Gao, Mingyan Jiang, Yixiong Yang, Sujia Wang, Chen Chen, Zhenghua Luo, Bingyang Lyu, Qibing Chen","doi":"10.1016/j.ecoenv.2025.118704","DOIUrl":"10.1016/j.ecoenv.2025.118704","url":null,"abstract":"<div><div>Currently, phytoremediation technology is widely used in the management of Pb-contaminated soil, and plants with high Pb tolerance can adapt to the environment through tolerance mechanisms, which can promote the sustainable development of soil health. In this study, we revealed the Pb tolerance of two species of <em>Indocalamus</em> in different levels of Pb-contaminated soil (0, 500 and 1500 mg/kg) and the effects of rhizosphere environmental factors on tolerance. The BCF and TF of Ped (<em>Indocalamus pedalis</em>) did not change with increasing concentration, whereas the TF of Kun (<em>Indocalamus kunmingensis</em>) decreased to reduce the upwards transport of Pb, and the GSH content of Kun clearly increased. Pb in plant cells was distributed mainly in the cell wall of Kun, as well as in the roots, whips, and stems of Ped, whereas Pb in the leaves of Ped was distributed mainly in the vesicles. In terms of Pb forms, the proportion of highly toxic Pb was greater in Ped than in Kun, which was attributed mainly to its transport through stems. In terms of the rhizosphere environment, the rhizobacteria of Ped contributed to only higher Aci-Pb ratios, and the rhizobacteria of Kun led to an increase in the overall amount of Pb available for uptake by the plant. Overall, Kun was more tolerant to Pb in Pb-contaminated soil than Ped was. Kun tolerated Pb stress in the following four main ways: 1) limiting Pb to underground parts, 2) increasing physiological tolerance, 3) blocking Pb outside the cell wall, and 4) decreasing Pb toxicity. In addition, rhizobacteria affected Pb uptake to different degrees by promoting Pb activation in the rhizosphere soil. Therefore, Kun can be used as a potential plant for the remediation of Pb-contaminated soil, and the results of this study further provide some guidance for improving Kun remediation efficiency.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118704"},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chengcai Kong , Rong Li , Xiaoying Wang , Lijuan Li , Nannan Kang , Xin Zhen , Yishan Dong , Guijun Yan
{"title":"Environmental aminomethylphosphonic acid (AMPA) exposure increased the risk of spontaneous abortion through lactate-induced JunB lactylation in trophoblast","authors":"Chengcai Kong , Rong Li , Xiaoying Wang , Lijuan Li , Nannan Kang , Xin Zhen , Yishan Dong , Guijun Yan","doi":"10.1016/j.ecoenv.2025.118743","DOIUrl":"10.1016/j.ecoenv.2025.118743","url":null,"abstract":"<div><div>The etiology of spontaneous abortion (SA) is complex, and the underlying mechanisms remain largely elusive. Environmental toxins have been implicated in the increased occurrence of SA. However, the association between aminomethylphosphonic acid (AMPA) exposure and SA has not yet been established. We found that AMPA, along with the lactate were significantly elevated in villous tissues from SA patients compared to normal controls, with a robust positive correlation between AMPA and lactate concentrations. More importantly, AMPA exposure induced SA in C57BL/6 mice probably through the dysfunctions of human trophoblast cell. Further studies indicated that the L-lactate production which can be induced by AMPA via PPARγ/ANGPTL4 pathway caused similar defects of human trophoblast cells. A global elevation of protein lactylation has been detected in the villous tissues from SA patients as well as AMPA or lactate treated human trophoblast cells. Pan-Kla antibody coimmunoprecipitation coupled with mass spectrometry of AMPA or lactate treated human trophoblast cells revealed JunB, which was reduced in the placenta villus from SA patients and AMPA/lactate treated human trophoblast cells, could be lactylated at lysine (K) 36. JunB K36R mutation abolished JunB lactylation and ameliorated AMPA induced JunB loss via ubiquitination in human trophoblast cells. In comparison to wild type JunB, JunB K36R mutation had better protective roles in AMPA induced trophoblast dysfunctions. In conclusion, our results demonstrate that AMPA exposure promotes lactate production via the PPARγ/ANGPTL4 pathway, which subsequently inhibits the proliferation, migration and invasion of trophoblasts through JunB K38 lactylation, ultimately leading to SA.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"Article 118743"},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unveiling BPS-induced colonic inflammatory injury in female mice: Integrated evidence from colon microbiome and metabolomic analyses.","authors":"Han Liu, Yutian Wang, Lisi Wei, Jing Xu, Ruirui Wang, Ling-Guo Zhao, Zhi Tang","doi":"10.1016/j.ecoenv.2025.118747","DOIUrl":"https://doi.org/10.1016/j.ecoenv.2025.118747","url":null,"abstract":"<p><p>Bisphenol S (BPS), a common alternative to bisphenol A (BPA), is extensively utilized in the production of food-contact materials. Concerns about its potential health impacts have grown. However, the effects of BPS exposure on colonic physiology and its underlying molecular mechanisms remain poorly characterized. This study aimed to elucidate the effects of BPS exposure on gut health in female mice, focusing on investigate the role of colon microbiome and metabolome involved in these effects. The effects of BPS exposure on female mice were evaluated via oral gavage administration at doses of 0.05 mg/kg/day and 5 mg/kg/day for four weeks. Fecal samples and colon tissue collected from BPS exposure and control group were subjected to 16S rRNA gene sequencing and GC-MS based metabolomic analysis. Our results show that BPS exposure caused typical colonic damage, including shortened colon length, inflammatory responses, and weakened gut barrier function. Significant alterations were observed in gut microbiota composition, showing imbalances between harmful and beneficial bacteria, with a significant decrease in genera such as Paraprevotella, Ruminococcaceae NK4A214 group, Ruminiclostridium 6, and an increase in Escherichia, Helicobacter, Parasutterella, Erysipelatoclostridium, and Achromobacter. Moreover, metabolic pathways associated with colonic inflammation, including tryptophan metabolism, glutamate metabolism, and fatty acid metabolism, were significantly altered. Our findings demonstrate that BPS exposure compromises colonic homeostasis, induces dysbiosis of the gut microbiota, and disrupts colonic metabolic activity. These findings may provide critical molecular insights regarding disruption of gut integrity as a potential new pathway for mitigating human health risks associated with bisphenol S exposure.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118747"},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}