Ecotoxicology and Environmental Safety最新文献

{"title":"Plot-scale observation on antibiotics migration in surface runoff and leachate from chicken-raising orchard of Entisol during rainstorms","authors":"Xin-Yu Liu ,&nbsp;Lanre Anthony Gbadegesin ,&nbsp;Yang He ,&nbsp;Jian-Qiang Zhang ,&nbsp;Chen Liu","doi":"10.1016/j.ecoenv.2025.118105","DOIUrl":"10.1016/j.ecoenv.2025.118105","url":null,"abstract":"<div><div>Field investigation on manure-sourced veterinary antibiotics migrating via runoff processes under natural rainfalls is quite limited due to hydrological complexity. The impact of manure application on antibiotics migration in soil is also scarcely discussed at the plotscale. This study examined the dynamic changes of concentration and mass flux of eight antibiotics migrating from chicken-raising orchards during rainstorms. Results showed instantaneous presence of antibiotics in the flow samples collected upon generation of surface runoff and leachate. Their concentrations responded strongly towards the flow rate, with concentration peaks mostly overlapping with flow peaks. Chicken-raising treatment resulted in significantly higher runoff generation than orchard without chickens. The total mass flux of antibiotics reached up to 5.46 and 9.41 μg/(m²·h) for surface runoff and leachate respectively, with sulfonamides (SAs) showing the highest migration (0.02–4.52 μg/(m²·h)) and tetracyclines (TCs) and quinolones (QLs) the lowest (10⁻⁴–10⁻¹ μg/(m²·h)). Leaching performed as an important pathway for contaminant migration in the studied soil, with mass fluxes 1.5–11 times higher than those of surface runoff, and manure-derived colloids accelerated antibiotics leaching. pH, colloid concentration and instantaneous rainfall intensity were the primary influencing factors, all displaying positive correlations with antibiotics migration (<em>p</em> &lt; 0.05). This study implied that raising chickens in orchard poses a risk of antibiotic contamination to surrounding ecosystem. It also highlighted the importance of exploring soil hydrological process in assessing their migration, particularly the previously underestimated contribution of subsurface flow via leaching.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118105"},"PeriodicalIF":6.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect and potential mechanisms of per- and polyfluoroalkyl substances (PFAS) exposure on kidney stone risk","authors":"Ying Cui ,&nbsp;Aitong Wu ,&nbsp;Hao Liu ,&nbsp;Yuanyuan Zhong ,&nbsp;Kefan Yi","doi":"10.1016/j.ecoenv.2025.118087","DOIUrl":"10.1016/j.ecoenv.2025.118087","url":null,"abstract":"<div><div>Exposure to per- and polyfluoroalkyl substances (PFAS) may be associated with an increased prevalence of some kidney diseases. Kidney stones are common and have a high prevalence of kidney diseases. However, there is no evidence for the effect and potential mechanisms of PFAS on kidney stone risk. In this study, we designed a cross-sectional study using the National Health and Nutrition Examination Surveys (NHANES) data from 2017 to 2020. Our results revealed that PFAS were positively associated with kidney stone risk, and PFDA was the main contributing compound among PFAS. The triglyceride-glucose (TyG) index and the systemic immune-inflammatory (SII) index had significant mediation effects. In addition, target proteins, such as IL-6, TNF, ALB, IL-1B, and AKT1, and signaling pathways, including TNF and IL-17 pathways, might be potential mechanisms of PFAS in promoting kidney stone risk. In conclusion, PFAS, especially PFDA, increases the risk of kidney stones by the mediation effects of the TyG index and SII index. TNF and IL-17 signaling pathways may be potential mechanisms. Our findings provide new evidence for the effects and potential mechanisms of PFAS exposure in increasing kidney stone risk. However, in the future, it is still imperative to further explore and validate the underlying mechanisms of PFAS-induced kidney stone formation through experimental studies.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118087"},"PeriodicalIF":6.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The selection of priority control perfluorinated alkyl substances in drinking water source of Guangzhou, China, a case study based on health risks ranking","authors":"Jingru Zhang ,&nbsp;Sijia Liu ,&nbsp;Xuan Yu ,&nbsp;Qianqian Yu ,&nbsp;Lingyun Yu ,&nbsp;Fanqi Jing ,&nbsp;Han Zhang ,&nbsp;Zhanlu Lv ,&nbsp;Ling-Chuan Guo ,&nbsp;Lulu Zhang","doi":"10.1016/j.ecoenv.2025.118092","DOIUrl":"10.1016/j.ecoenv.2025.118092","url":null,"abstract":"<div><div>The health risk of perfluorinated alkyl substances (PFASs) in drinking water always deserve attention, which involve the changing evaluation criteria and nonnegligible mixed exposure of multiple PFASs. Currently, the United States Environmental Protection Agency is reducing the maximum contaminant levels. At the same time, insufficient attention to mixed exposure may underestimate the overall risks of PFASs in drinking water. To face these challenges, a scheme, which identify a priority towards the most problematic compounds and accurate manage PFASs in drinking water are warranted. Liuxi River drinking water source was taken as a case study, and the levels of 13 PFASs were analyzed. A data-driven ranking model, which considered new maximum contaminant levels and mixed exposure, was used to choose priority control PFASs. PFASs were divided into four levels according to their health risks and drinking water intake rate; the compounds at Level I (perfluorononanoic acid) need priority control, while compounds at Levels II (perfluorododecanoic acid, perfluorooctane sulfonate, and perfluoroundecanoic acid) and Level III (perfluoroheptanoic acid, perfluorooctanoic acid, and perfluorobutane sulfonate) deserve more attention. This study provides a solid scientific basis for selecting priority control PFASs in drinking water sources, for the purpose of adequately ensuring the safety of drinking water under the lowest management cost. The results also call for more consideration on feasibility of current standards of PFASs in drinking water.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118092"},"PeriodicalIF":6.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triclosan exposure induces liver fibrosis in mice: The heterogeneous nuclear ribonucleoprotein A1/pyruvate kinase M2 axis drives hepatic stellate cell activation","authors":"Xiao Ni ,&nbsp;Ziyun Wei ,&nbsp;Yuxuan Peng ,&nbsp;Linlin Zheng ,&nbsp;Jianing Shang ,&nbsp;Fu Liu ,&nbsp;Yunwei Li ,&nbsp;Jieyu Liu","doi":"10.1016/j.ecoenv.2025.118113","DOIUrl":"10.1016/j.ecoenv.2025.118113","url":null,"abstract":"<div><div>Triclosan (TCS) is an effective broad-spectrum antibacterial agent. TCS possesses a stable structure, can easily accumulate in the environment, and may have numerous negative impacts on human health. One organ particularly susceptible to TCS damage is the liver; however, the molecular mechanisms underlying TCS-induced liver damage remain unclear. A long-term TCS exposure model was established in C57BL/6 mice through maternal administration from gestation to postnatal 8-week-old. The offspring were randomly assigned to three groups (0, 50, and 100 mg/kg TCS) with six animals per group, ensuring an equal gender distribution (3 males and 3 females). The results showed that TCS-exposed mice exhibited serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzyme activities increased by 1.5–2 times when compared with vehicle-treated mice, along with features of liver fibrosis. In the LX-2 cell line, used as an <em>in vitro</em> model, TCS promoted proliferation and migration and induced the activation of hepatic stellate cells (HSCs). The level of pyruvate kinase M2 (PKM2) dimer increased by 200 % in LX-2 cells treated with TCS. PKM2 dimer overexpression stimulated HSC activation, whereas treatment with TEPP-46 (a PKM2 dimer inhibitor) significantly decreased the activation process. The expression of heterogeneous ribonucleoprotein particle A1 (hnRNPA1) was upregulated in the TCS treatment group and promoted the PKM2 expression. Moreover, disruption of the hnRNPA1/PKM2 axis reduced HSC proliferation and migration activated by TCS. Overall, our findings highlighted that TCS could cause liver fibrosis by stimulating the proliferation and migration of HSCs activated via the hnRNPA1/PKM2 axis, providing promising treatment options for TCS-related liver damage.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118113"},"PeriodicalIF":6.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling heavy metals distribution in surface sediments from the perspective of coastal industrial structure and their impacts on bacterial communities","authors":"Lei Jin ,&nbsp;Lei Ding ,&nbsp;Yao Zhang ,&nbsp;Tiejun Li ,&nbsp;Qin Liu","doi":"10.1016/j.ecoenv.2025.118098","DOIUrl":"10.1016/j.ecoenv.2025.118098","url":null,"abstract":"<div><div>Heavy metal pollution of marine sediments along the coastal industrial parks have always received extensive attention due to their persistent hazard to local marine ecosystem. Despite this, our knowledge about the influence of geography and coastal industrial structures on heavy metal distributions remains little. In this study, surface sediment samples were collected from the coastal zone of the industrial park in Ningbo. The physicochemical properties, heavy metals with ecological risk levels and bacterial structures as well as their relationships in these sediments were comprehensively analyzed. We found that: heavy metal concentrations of surface sediment revealed wide variation between this study sea area and other coastal economic areas; increasing attention should be paid to the Cu, Hg, Cd and As pollution due to their high contamination degree and environment risk; the distribution of heavy metals is closely related to the geographic location and nearshore industrial structures; the physicochemical features (e.g., TN, PHCs and pH) of sediments could better explain the occurrence characteristics of heavy metals present; individual metals (Cu and Cr) significantly affected the bacterial α-diversity; Cr inhibits multiple functional pathways associated with energy metabolism and pollutant degradation; RDA analysis and co-occurrence network confirmed that several heavy metals (especially Zn, Cr, Cu and Cd) exhibited large effects on bacterial community structure; moreover, genera <em>Idiomarina Sulfurovum</em> and <em>Sulfurimonas</em> could be used as biological indicators for specific heavy metals contamination in our study. Our findings provide a novel insight to understand the heavy metal distribution and bacterial variation associated with industrial activities.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118098"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology, molecular docking, and animal studies to investigate the protective effect of astragalus polysaccharide on fluoride-induced renal injury in rats","authors":"Xiaoyue Zhang ,&nbsp;Qianda Yan ,&nbsp;Yuanyuan Xiao ,&nbsp;Xingyan Du ,&nbsp;Xuehua Zhang ,&nbsp;Didong Lou ,&nbsp;Fang Peng ,&nbsp;Daiyong Chen ,&nbsp;Wenchao Tang","doi":"10.1016/j.ecoenv.2025.118109","DOIUrl":"10.1016/j.ecoenv.2025.118109","url":null,"abstract":"<div><div>Fluoride is an essential trace element required for normal physiological functions and holds significant importance for human health. However, excessive fluoride intake can lead to renal damage, for which effective prevention and therapeutic strategies remain scarce. Astragalus polysaccharide (APS), a major bioactive component of the traditional Chinese herb <em>Astragalus membranaceus</em>, possesses pharmacological properties including anti-inflammatory, antiviral, and antioxidant activities. In this study, we investigated the protective effects of APS against fluoride-induced renal injury in vivo experiment. Additionally, network pharmacology and molecular docking techniques were employed to predict its potential mechanisms of action, while the protein expression levels of key target molecules were validated. The results demonstrated that APS intervention significantly alleviated renal injury and oxidative stress induced by sodium fluoride (NaF) in rats. Key targets involved in the amelioration of fluoride-induced renal damage by APS included STAT3, Caspase-3, JUN, MMP1, and PTGS2. Molecular docking analysis revealed high-affinity binding between APS and these core targets. Immunohistochemical and Western blot analysis further confirmed that APS suppressed the expression of pro-apoptotic proteins STAT3, Caspase-3, JUN, and MMP1 while enhancing the expression of the anti-apoptotic protein PTGS2. Overall, our findings suggest that APS alleviates fluoride-induced renal injury by modulating multiple targets, with the potential mechanism linked to the regulation of apoptotic processes. This study provides a theoretical basis for the prevention and treatment of fluoride toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118109"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow mesenchymal stem cells senescence induced by LCCP through activation of cGAS-STING-mediated inflammation","authors":"Xiao Xiao,&nbsp;Song Wang,&nbsp;Xin Zhang,&nbsp;Junyong Zheng,&nbsp;Dazhi Yang,&nbsp;Songlin Peng","doi":"10.1016/j.ecoenv.2025.118069","DOIUrl":"10.1016/j.ecoenv.2025.118069","url":null,"abstract":"<div><div>The pollution issue of chlorinated paraffins has garnered widespread attention, with short-chain chlorinated paraffins (SCCPs) being comprehensively banned by the Stockholm Convention due to environmental contamination concerns. Currently, long-chain chlorinated paraffins (LCCPs) are widely used, necessitating an assessment of their toxicological impact. Bone marrow mesenchymal stem cells (MSCs) are a type of multipotent stem cell with self-renewal, immunoregulatory, and tissue repair capabilities, exhibiting important biological functions. In this study, MSCs were used as a model to evaluate the toxicological effects of LCCP. Initially, cell proliferation experiments were conducted to assess the impact of LCCP on MSC proliferation. The results indicated a significant reduction in cell proliferation capacity. Further work revealed that LCCP treatment induced cell senescence, as evidenced by Sa-β-gal staining and the evaluation of senescence markers, including p16, p21, and p53. Furthermore, LCCP treatment led to inflammation and oxidative stress, as analyzed by corresponding marker molecules, and ultimately resulted in cell death. Based on these findings, we investigated the molecular mechanisms underlying LCCP-induced MSC senescence. In summary, this paper is the first study to systematically investigate the toxicological effects of LCCPs on stem cells. The current study demonstrates that LCCP induces MSC senescence, highlighting its potential toxicity. This research lays the foundation for further elucidating the toxicology of LCCP. This finding suggests that the use of LCCPs should be strictly controlled under rigorous regulation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118069"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum testosterone partially mediates the adverse effects of organophosphate pesticides exposure on growth indicators of children and adolescents","authors":"Zhenao Zhang ,&nbsp;Xiaoya Sun ,&nbsp;Jingyi Ren ,&nbsp;Yadong Zhang,&nbsp;Rui Wen,&nbsp;Huanting Pei,&nbsp;Siqi Zhu,&nbsp;Bowen Yin,&nbsp;Ziyi Wang,&nbsp;Yuxia Ma","doi":"10.1016/j.ecoenv.2025.118073","DOIUrl":"10.1016/j.ecoenv.2025.118073","url":null,"abstract":"<div><div>Environmental pollutants affect children’s and adolescents’ growth. However, the effect of organophosphate pesticides (OPPs) exposure on this population remains unclear. This study applied a comprehensive statistical framework to evaluate the effects of multiple OPP metabolites on growth indicators in individuals aged 6–19 using data from the National Health and Nutrition Examination Survey (NHANES). The framework integrated latent class analysis (LCA), survey-weighted generalized linear regression models (SWGLMs), weighted quantile sum (WQS) regression, and bayesian kernel machine regression (BKMR). The role of total testosterone (TT) as a mediator was assessed. Network pharmacology methods were used to identify key targets and therapeutic drugs. LCA identified three exposure patterns for OPP metabolites: High DMP-High DEP (N = 145), Low DMP-Low DEP (N = 689), and High DMP-Low DEP (N = 97). SWGLMs, WQS, and BKMR demonstrated a negative association between OPPs exposure and growth indicators, particularly in females and adolescents. TT mediated this association, with mediation proportions ranging from 8.7 % to 35.7 %. Network pharmacology analysis identified 120 key targets, and pterostilbene emerged as a potential therapeutic agent. These findings suggest that OPPs exposure adversely affects children’s and adolescents’ growth, partly mediated by TT. Adolescents and females may be particularly vulnerable. Pterostilbene supplementation represents a potential strategy to mitigate these adverse effects.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118073"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of glyphosate exposure with frailty and all-cause mortality in general adults: A population-based cohort study","authors":"Tongtong Yang ,&nbsp;Liuhua Zhou ,&nbsp;Peng Jing ,&nbsp;Yulin Bao,&nbsp;Lingfeng Gu,&nbsp;Yixi Chen,&nbsp;Xinying Shi,&nbsp;Hao Wang,&nbsp;Liansheng Wang ,&nbsp;Sibo Wang","doi":"10.1016/j.ecoenv.2025.118096","DOIUrl":"10.1016/j.ecoenv.2025.118096","url":null,"abstract":"<div><h3>Background</h3><div>Glyphosate is the most widely used herbicide globally. However, its association with frailty, an emerging public health concern, and mortality in the general population remains unclear.</div></div><div><h3>Methods</h3><div>This cohort utilized the National Health and Nutrition Examination Survey (NHANES) 2013–2018 data, with follow-up through December 31, 2019. Weighted logistic regression, Cox regression, and restricted cubic splines analyses were performed to investigate the association of urinary glyphosate with frailty, defined using a 49-item frailty index at baseline, and all-cause mortality, respectively. Causal mediation analysis and sensitivity analysis were also employed.</div></div><div><h3>Results</h3><div>4697 adults (mean age: 47.1 years; 48.9 % male) were included. Multivariable logistic regression showed that elevated urinary glyphosate correlated with increased frailty prevalence (log₂-transformed, adjusted OR = 1.14, 95 % CI: 1.04–1.25; quartiles, <em>P</em> for trend = 0.002), with a linear dose-response relationship (<em>P</em> value for nonlinearity = 0.568). During a median follow-up of 3.9 years, 238 total deaths occurred. Multivariable-adjusted Cox regression demonstrated that urinary glyphosate was positively related to all-cause mortality (log₂-transformed, adjusted HR = 1.19, 95 % CI: 1.07–1.31; quartiles, <em>P</em> for trend = 0.005). Additionally, frailty significantly mediated the association between glyphosate and all-cause mortality, with mediation proportion of 30.3 %.</div></div><div><h3>Conclusions</h3><div>Urinary glyphosate was positively associated with frailty and all-cause mortality in the general US adult population. Frailty also played a partial mediator role in this relationship. These findings provide new evidence of adverse health effects of glyphosate and imply that enhanced efforts should be made to mitigate exposure to glyphosate.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118096"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of antibiotic resistance gene rebound by degrees of microecological niche occupation by microbiota carried in additives during the later phases of swine manure composting","authors":"Xiaoxia Hao ,&nbsp;Weiping Sang ,&nbsp;Fengting Li ,&nbsp;Linyuan Shen ,&nbsp;Li Zhu ,&nbsp;Li Rong ,&nbsp;Dongmei Jiang ,&nbsp;Lin Bai","doi":"10.1016/j.ecoenv.2025.118112","DOIUrl":"10.1016/j.ecoenv.2025.118112","url":null,"abstract":"<div><div>The occupation of microecological niches (MNs) by bacteria carrying lower antibiotic resistance genes (ARGs) has been demonstrated an effective strategy for reducing ARGs in compost, thereby mitigating the associated land use risks. In this study, humus soil (HS), matured compost (MC), and their respective isolated microbial agents (HSM and MCM), which exhibit varying abundances of ARGs, were introduced as additives after the thermophilic phase to investigate their influence on ARG removal and the mechanisms underlying effective MN occupation. The addition of HS resulted in the most favorable outcomes, including the highest carbon degradation, minimized nitrogen loss, and an 83.16 % reduction in ARG abundance during the later composting stages. In comparison, ARG rebound levels were 61.77 %-285.33 % across other treatments and 729.23 % in the control. Distinct dominant bacterial genera and potential ARG-host bacterial communities were observed, which varied with different additives and contributed to MN occupation dynamics. The addition of the HS additive intensified competition among non-host bacteria, and diversified the interactions both between genes and between bacteria. These changes suppressed horizontal gene transfer (HGT) mediated by mobile genetic elements (MGEs) and altered the abundance and composition of both dominant and non-dominant potential host species. Furthermore, it shifted the relative importance of key physicochemical parameters, collectively enhancing ARG removal during composting. These findings elucidate the mechanisms by which MN adjustments contribute to ARG reduction, providing actionable insights for designing composting strategies that mitigate environmental ARG dissemination risks more effectively.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"294 ","pages":"Article 118112"},"PeriodicalIF":6.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}