ACS Measurement Science Au最新文献

{"title":"Quantifying Ultra-Low Pigment Solubilities by UV–Vis Spectroscopy: Implications for Toxicological Assessment","authors":"Lukas J. Patalag","doi":"10.1021/acsmeasuresciau.5c00214","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00214","url":null,"abstract":"<p>Accurate toxicological risk assessment of organic pigmentsis oftenhampered by their extremely low and poorly characterized solubilities,which obscure their true molecular bioavailability in biological systems.We present a comprehensive optical spectroscopic study of 18 pigmentsfrom 7 different structural classes, determining ultralow solubilitiesin DMSO and NMP through a novel iterative dilution procedure thatdeconvolutes particulate and molecular spectral signatures based onexcitonic coupling theory. Rigorous exclusion of particulate contributionsyielded true molecular extinction coefficients and optical band shapes.Results were consolidated by computational simulations of the molecularand crystalline pigment forms, respectively, at the TDDFT (PBE0/def2-SVP)level of theory. To translate these findings to biology, experimentallyderived solubilities were extended to water and cytosol by integratingAbraham-type solvation models with composition-based pp-LFER frameworks.This combined approach revealed subnanomolar to subpicomolar solubilitiesin aqueous media and upper-bound cytosolic concentrations typicallybelow 1 μM, providing a physicochemical basis to criticallyassess the relevance of toxicological end points, such as sensitizationand genotoxicity, for tattooing and patch testing. Our results suggestthat many negative toxicological findings may reflect insufficientbioavailability rather than a lack of intrinsic hazard.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"957–971"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Lipidomic Profiling of Kidney Functional Units in SIV-Infected and ART-Treated Rhesus Macaques","authors":"Kyle A. Vanderschoot,&nbsp;Cory J. White,&nbsp;Kelli A. Steineman,&nbsp;Christopher M. De Caro,&nbsp;Dionna W. Williams,&nbsp;Elizabeth K. Neumann","doi":"10.1021/acsmeasuresciau.6c00074","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00074","url":null,"abstract":"<p>Human immunodeficiencyvirus (HIV) disrupts lipid metabolism,contributingto renal dysfunction seen in acute kidney injury (AKI), chronic kidneydisease (CKD), and HIV-associated nephropathy (HIVAN). While antiretroviraltherapies (ARTs) slow viral replication, prolonged use causes lipiddysregulation, leading to nephrotoxicity. Lipid metabolism has beenhypothesized to be altered through viral manipulation, altering themetabolism, storage, and physical properties of lipids. These manipulationshave been observed to generate increased levels of polyunsaturatedfatty acids, cholesterol/sphingomyelin domains, and pro-inflammatoryphospholipid tails. Since the kidney is composed of a network of spatiallydistinct functional units, each with a unique physiological role anddifferential vulnerability to infection and drug toxicity, methodscapable of preserving native molecular context are critical for identifyingregion-specific perturbations. In this paper, we investigate the lipidomicchanges in simian immunodeficiency virus (SIV)-infected and ART-treatedrhesus macaque kidneys using matrix-assisted laser desorption/ionizationmass spectrometry imaging (MALDI MSI). By using this approach, wecan analyze discrete tissue functional units, such as glomeruli, proximaltubules, distal tubules, and collecting ducts, and gain insights intohow viral infection and ART reshape lipid metabolism. Through thiswork, we further elaborate on the lipidomic changes in glomeruli specifically,identifying the critical role that sphingomyelins play in the contextof viral fusion and budding, in addition to other phospholipids andtheir role in altered oxidative phosphorylation and inflammatory signaling.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1155–1166"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Focusing SIMS Enables Quantitative Dopant Analysis in Nanoscale Silicon Devices beyond Conventional Spatial Resolution Limits","authors":"Valentina Spampinato,&nbsp;Alexis Franquet,&nbsp;Paul van der Heide","doi":"10.1021/acsmeasuresciau.6c00086","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00086","url":null,"abstract":"<p>The continuous downscaling of semiconductor devices hascreateda pressing need for analytical methodologies capable of enabling processcontrol in confined volumes and sub-100 nm features. Conventionalsecondary ion mass spectrometry (SIMS), while highly sensitive, lacksthe spatial resolution required for such applications. Nevertheless,SIMS remains a powerful tool for the analysis of small features throughthe self-focusing (SF) SIMS concept, which exploits the formationof cluster ions that inherently localize chemical information withinthe region of interest. In this study, SF-SIMS is applied to the quantificationof boron in patterned samples composed of boron-doped silicon finswith widths ranging from 500 to 20 nm, embedded in boron-doped siliconoxide. By selecting cluster ions that originate exclusively from thesilicon fin region, the spatial resolution limitations of conventionalSIMS are overcome without compromising the sensitivity. Using thisapproach, a boron implant with a peak concentration of ∼9 ×10²⁰ at/cm³ was measured in the widest fins(500 nm), in good agreement with SRIM simulations and conventionalSIMS methods, while a concentration of ∼4.5 × 10²⁰ at/cm³ was obtained for the narrowest fins (20 nm). Thisstudy establishes SF-SIMS as a reliable, rapid, and preparation-freeapproach for dopant quantification in nanoscale semiconductor devicesdown to 20 nm.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1216–1222"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Background-Free, Label-Free, and Reproducible SERS via Plasma–Halide-Engineered Gold Nanoparticles","authors":"Yen Ling Chiu,&nbsp;Ya Chi Chen,&nbsp;Loid Elizabeth Urtecho Navas,&nbsp;Wei-Hung Chiang","doi":"10.1021/acsmeasuresciau.5c00196","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00196","url":null,"abstract":"<p>Surface-enhanced Raman spectroscopy (SERS) is often limitedbysubstrate-derived background from organic ligands (e.g., citrate),which degrades spectral fidelity, sensitivity, and quantitation. Wereport a measurement-oriented SERS platform based on size-tuned, microplasma-synthesizedgold nanoparticles (AuNPs, 14–100 nm) that are surface-purifiedwith bromide (Br^–). Bromide competitively displacesweakly bound citrate while remaining Raman-inactive, thereby suppressingbaseline features in the 1000–1800 cm^–1 fingerprintregion without introducing new peaks. An optimal Br^– window (∼1.5 mM, ∼3 h) achieves effective cleaningwhile avoiding surface passivation. The resulting substrates deliverstrong enhancement (EF &gt;10⁶), subnanomolar limits ofdetection(10^–9 M for rhodamine 6G; 10^–10 M for malachite green), and wide linear ranges (10^–9–10^–4 and 10^–10–10^–4 M, respectively). Spot-to-spot variability is low(RSD &lt;∼8%), and activity is retained over weeks (&gt;90%signalafter storage), underscoring practical reproducibility and stability.Spectroscopic and surface charge analyses corroborate ligand displacementand improved adsorption on clean Au surfaces while preserving plasmonicresponse. This simple, green, and scalable plasma–halide strategyprovides background-free, label-free, and reproducible SERS measurements.Driven by electrostatic complementarity, this platform offers a highlyeffective route for the quantitative trace analysis of cationic analytes,relevant to environmental monitoring and chemical safety.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"937–948"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-Induced Breakdown Spectroscopy and Chemometrics of Diffuser Alloys for Tritium Production and Byproduct Purification","authors":"John T. Kelly,&nbsp;William W. Holbrook,&nbsp;William E. Gilbraith,&nbsp;Christopher J. Koch,&nbsp;Jennifer K. Naglic,&nbsp;Daniel M. Clairmonte,&nbsp;Lucas M. Angelette","doi":"10.1021/acsmeasuresciau.6c00100","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00100","url":null,"abstract":"<p>This study evaluates the potential of laser-induced breakdownspectroscopyfor real-time monitoring of palladium and palladium-based alloys usedfor tritium recovery. Co-evolving gases include residual oxygen, nitrogen,methane, water, carbon monoxide, and carbon dioxide in the manufacturingof tritium at the Savannah River Site from its establishment. Palladiummetal and palladium alloys were analyzed with emphasis on the 320–350nm spectral region, where relative intensity ratios among key emissionlines provide compositional insight. Spectral deconvolution was performedusing the atomic emission database to resolve overlapping peaks tounveil spectral signatures of Ru (emission at 343.6681 nm) neighboringdominant palladium features. Given the complexity of the resultingspectral data sets, machine learning techniques were applied to enhanceinterpretability and predictive performance. Principal componentsanalysis and partial least-squares were employed for dimensionalityreduction and classification modeling for their ability to capturenonlinear relationships and improve classification accuracy. Theseapproaches were developed as preliminary models to provide an initialspectral library for the highest predictive accuracy for tritium impurityremoval and diffuser process upsets. Our advancements and progressaim at employing machine learning as a powerful noninvasive diagnosticfor monitoring gas–metal interactions and impurity removalin tritium production systems that integrate chemometrics and adaptivedecision-making strategies developed in this study.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1256–1262"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hyperbranched Rolling Circle Amplification Assay Using Aptamer–Hemin/G-Quadruplex Recognition for the Detection of Creatine Kinase-MB","authors":"Ensiyeh Mirsadoughi,&nbsp;Sayantan Tripathy,&nbsp;Fatemeh Zare,&nbsp;Gerard L. Cote,&nbsp;Samuel Mabbott","doi":"10.1021/acsmeasuresciau.6c00016","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00016","url":null,"abstract":"<p>Creatine kinase-myocardial band (CK-MB) remains an importantbiomarkerfor the early diagnosis and clinical management of myocardial infarction.Here, we report a sensitive colorimetric assay for CK-MB based ona conformation-locked aptamer guanine-quadruplex (G-quadruplex) systemintegrated with magnetic nanoparticle capture and hyperbranched rollingcircle amplification (HRCA). Target binding induces conformationalunlocking of the aptamer, initiating HRCA-mediated signal amplificationand generating a concentration-dependent colorimetric response inthe presence of a chromogenic substrate. A compact 3D-printed imagingmodule, combined with a Python-based processing pipeline, was usedto extract grayscale intensity values from assay images for quantitativeanalysis. Using only 25 μL of sample, the assay achieved limitsof detection (LOD) of 0.268 ng/mL in aqueous samples and 0.328 ng/mLin blood-spiked samples, and limits of quantification (LOQ) of 0.89ng/mL and 1.09 ng/mL, respectively, with a linear dynamic range of0.5–100 ng/mL. Grayscale intensity was inversely proportionalto CK-MB concentration, enabling quantitative detection across clinicallyrelevant levels. The HRCA amplification process and target-inducedactivation were further validated by TapeStation gel electrophoresis,confirming the formation of amplified DNA nanostructures. This platformprovides a low-volume, amplification-based strategy for CK-MB detectionthat is compatible with simple optical readout and has strong potentialfor point-of-care applications.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"972–982"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Two-Step Prioritization Framework for the Nontargeted LC-HRMS Analysis of Chemicals of Environmental Concern and Byproducts during Drinking Water Production","authors":"Lapo Renai,&nbsp;Giulia Bonaccorso,&nbsp;Federico Sinigaglia,&nbsp;Andrea Ravalli,&nbsp;Daniela Santianni,&nbsp;Massimo Del Bubba","doi":"10.1021/acsmeasuresciau.6c00061","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00061","url":null,"abstract":"<p>The growing occurrence of chemicals of environmentalconcern (CECs)in surface and drinking water requires advanced analytical approachescapable of covering the broad chemical space of known and unknowncontaminants potentially present in these matrices. Nontargeted analysis(NTA) based on liquid chromatography coupled with high-resolutionmass spectrometry (LC-HRMS) is gaining popularity as it is virtuallycomprehensive and may provide high data quality. However, NTA hassome limitations in terms of sensitivity, which may limit its effectiveness,and offline prioritization processes are usually adopted to bringout the desired features from the vast array of NTA signals. A novelLC-HRMS-based two-stage prioritization framework is proposed to evaluatethe combined chemical load by dissolved organic matter and CECs ina drinking water treatment plant (DWTP). For the first time, an experimentaldesign (DoE) approach is adopted to optimize LC–HRMS acquisitionparameters for the online prioritization of features responsible forthe combined chemical load in the investigated samples. DoE was performedon a training set of 42 regulated CECs, characterized by a wide chemicalcoverage (e.g., log<em> D</em> at pH = 7 between −2.49and 7.38), identifying optimal experimental conditions for maximizingtheir NTA detection. DoE-optimized online prioritization was combinedwith offline prioritization, performed using multivariate and univariateanalyses, capturing significant trends in dissolved organic matterfeatures, known CECs (e.g., perfluoroalkyl substances), and unknowncompounds, tentatively identified as potential pesticide byproducts.Targeted analysis, performed on the CEC training set, provided a 100%match with the NTA approach, thus validating the proposed two-stepprioritization framework. The two-step prioritization allows for (i)improving method sensitivity thanks to the online prioritization and(ii) revealing both persistent and potential transformation-derivedcontaminants, thus providing a comprehensive tool for assessing chemicalload and treatment performance in DWTP.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1105–1115"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FERMO: A Dashboard for Biochemometric Prioritization of Molecular Features from Mass Spectral Data","authors":"Mitja M. Zdouc,&nbsp;Hannah E. Augustijn,&nbsp;Nataliia V. Machushynets,&nbsp;Lina M. Bayona,&nbsp;Sylvia Soldatou,&nbsp;Niek F. de Jonge,&nbsp;Sarah Casu,&nbsp;Marcel Jaspars,&nbsp;Gilles P. van Wezel,&nbsp;Marnix H. Medema,&nbsp;Justin J. J van der Hooft","doi":"10.1021/acsmeasuresciau.6c00022","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00022","url":null,"abstract":"<p>Many natural products can selectively modulate biologicalprocesses,making them prime candidates for drug discovery. However, the complexityof biological samples makes clear attribution of activity to moleculeschallenging, thereby hampering hypothesis-driven prioritization, withliquid chromatography-tandem mass spectrometry routinely detectinghundreds of molecules per sample. Existing biochemometric tools typicallyfocus on facilitating data-driven exploration to support manual interpretation,rather than more objective, data-driven prioritization and hypothesisgeneration. Here, we introduce FERMO, a free online dashboard interfacefor biochemometrics-based prioritization of molecular features andsamples. FERMO accepts qualitative and quantitative bioactivity assaydata and further integrates group metadata and results from genomemining. FERMO performs automated data processing, organization, andannotation, supporting prioritization with the calculation of customscores. FERMO supports both exploratory and targeted analysis throughefficient interactive visualization, reproducible prioritization,and data filtering. We demonstrate FERMO’s utility in benchmarkingstudies prioritizing bioactive natural products from complex biologicalmatrices. FERMO is freely available at https://fermo.bioinformatics.nl/.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1011–1022"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Sensitive DNA Bowtie Sensor for Calibration-Free Sex Hormone Detection Using Analyte-DNA Conjugates and Multiple Redox Labels","authors":"Asanka Gurukandure,&nbsp;Mainul I. Mazumder,&nbsp;Kacey G. Ortiz,&nbsp;Amanda S. N. Kurian,&nbsp;Subramaniam Somasundaram,&nbsp;Rashad R. Karimov,&nbsp;Christopher J. Easley","doi":"10.1021/acsmeasuresciau.6c00042","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00042","url":null,"abstract":"<p>Using a modular and reconfigurable DNA-based bowtie sensorstructure,several advancements in electrochemical (EC) biosensing are reported.Raw current and sensitivity were enhanced up to 13-fold using a novelredox reporter design, where a DNA strand was decorated with fivemethylene blue molecules (MB₅) instead of the typical singlelabel (MB), and flexible spacers further improved signal. Leveragingthe bowtie sensor’s modularity, custom synthesized analyte-DNAconjugates (testosterone-DNA, estradiol-DNA, and progesterone-DNA)and a control strand (digoxigenin-DNA) were incorporated into thecomplete DNA structure on electrode surfaces by enzymatic ligation.Upon binding antibodies, each of these sensors showed enhanced currentwith the MB₅ label, and responses consistently followeda Langmuir curve. A previously inaccessible signal-on response─sustainedcurrent at longer times─was observed at low square wave voltammetryfrequencies using MB₅, allowing new differential signalcorrections. The limit of detection (LOD) for direct antibody sensingwas as low as 0.08 nM (80 pM) for the antidigoxigenin antibody (controlsystem). A mathematical model was devised based on uncoupled equilibriaamong the analytes, antibodies, and the electrode surface. After trainingthe model on binding curves, calibration-free measurements of smallmolecule hormones were possible, with observed LODs for testosterone,estradiol, and progesterone at 0.2, 0.8, and 1 nM, respectively, extendingwithin human clinical ranges. For example, the testosterone sensor’srange was 200 pM to 70 nM, covering the clinical range in both females(0.52 to 2.43 nM) and males (9.0 to 34.7 nM), and the sensor functionswith 90% recovery in 50% human serum. This work expands the usageof the EC bowtie sensor, employing custom synthetic organic methodsto introduce new analyte-DNA conjugates and further validate the sensor’sgeneralizability, alongside developing a unique model of the bindingmechanism. Beyond this, the newly introduced MB₅ labelshould be functional with a range of other DNA-based EC sensors oraptamer-based sensors.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1056–1068"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native Mass Spectrometry of Soluble Proteins with Sodium Chloride Enabled by a Dendritic Detergent","authors":"Francesco Fiorentino,&nbsp;Wolf Hiller,&nbsp;Leonhard H. Urner","doi":"10.1021/acsmeasuresciau.6c00038","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.6c00038","url":null,"abstract":"<p>Salts are essential for maintaining native protein structureandbiomolecular interactions. Native mass spectrometry often yields uninterpretablespectra due to salt adduction and signal suppression. Existing workaroundsrequire buffer exchange or extensive cleanup, which can disrupt weaknoncovalent interactions and reduce throughput. Addressing this shortcoming,we establish a first-generation, dendritic triglycerol detergent asa simple-to-apply additive that enables native mass spectrometry ofbiophysically relevant protein concentrations in the presence of sodiumchloride. Adding this detergent to sodium chloride–containingsamples prior to electrospray ionization improves spectral quality,enhances signal-to-noise ratios, and enables an acquisition of interpretablenative mass spectrometry data for soluble proteins, including complexeswith lipids and drugs, at different ionic strengths. These improvementsare achieved without compromising native-like charge-state distributionsacross proteins ranging from myoglobin and lipopolysaccharide transportprotein H to bovine serum albumin and high-mass tetramers, such asalcohol dehydrogenase and pyruvate kinase. Nuclear magnetic resonancedata indicate a concentration-dependent sodium chloride complexationby detergent in solution, consistent with desalted protein complexesobservable in the gas phase. By rendering sodium chloride compatiblewith native mass spectrometry, first-generation, dendritic triglyceroldetergent enables the routine analysis of protein complexes at otherwiseinaccessible ionic strengths and biophysically relevant analyte concentrations.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"6 4","pages":"1038–1048"},"PeriodicalIF":14.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148769799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}