ACS Measurement Science Au最新文献

{"title":"Sample Preparation Approaches for Determination of Quinolones in Aqueous Matrixes: Systematic Review","authors":"Tainara Aparecida Nunes Ribeiro,&nbsp;Daiane Dulcileia Moraes de Paula,&nbsp;Marcella Matos Cordeiro Borges,&nbsp;Leandro Augusto Calixto and Keyller Bastos Borges*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0005610.1021/acsmeasuresciau.4c00056","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00056https://doi.org/10.1021/acsmeasuresciau.4c00056","url":null,"abstract":"<p >Quinolones and fluoroquinolones are among the most used antibiotics worldwide. Antibiotic resistance genes can be acquired by human pathogens from ambient microorganisms, which can lead to a treatment failure for bacterial infections. Furthermore, determining the levels of quinolones and fluoroquinolones in aqueous matrixes is crucial for safeguarding both human health and the environment. Using sample preparation techniques is essential since these compounds are commonly present in aqueous matrixes at trace levels. Therefore, we aimed to investigate the main analytical methods for the determination of quinolones in aqueous samples based on a systematic literature review. We only considered studies that presented more robust analytical techniques that allowed for more accurate and precise measurement of quinolones/fluoroquinolones in aqueous matrixes. In total, 18 articles met the inclusion criteria and were used in our analysis. A total of 21 quinolone antibacterial agents were investigated in water samples from 13 countries, showing a potential risk around the world. Ciprofloxacin (72.2%), enrofloxacin (61.1%), norfloxacin (50%), marbofloxacin, and levofloxacin (33.3%) were the most frequently evaluated compounds. High-Performance Liquid Chromatography stands out as the predominant instrumental technique for the separation and identification of these compounds. Additionally, among the selected studies in this review, 44% employed liquid–liquid extraction techniques and its miniaturized versions, while 56% opted for solid-phase extraction and its miniaturized variations. Finally, it is important to note that the final method efficiency relies on the entire process, from selecting the instrumental technique with appropriate detection limits, going through the entire sample preparation, to achieving a good recovery through adjustments in the extraction parameters to archive the determination of trace levels.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 1","pages":"19–30 19–30"},"PeriodicalIF":4.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143435816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample Preparation Approaches for Determination of Quinolones in Aqueous Matrixes: Systematic Review.","authors":"Tainara Aparecida Nunes Ribeiro, Daiane Dulcileia Moraes de Paula, Marcella Matos Cordeiro Borges, Leandro Augusto Calixto, Keyller Bastos Borges","doi":"10.1021/acsmeasuresciau.4c00056","DOIUrl":"10.1021/acsmeasuresciau.4c00056","url":null,"abstract":"<p><p>Quinolones and fluoroquinolones are among the most used antibiotics worldwide. Antibiotic resistance genes can be acquired by human pathogens from ambient microorganisms, which can lead to a treatment failure for bacterial infections. Furthermore, determining the levels of quinolones and fluoroquinolones in aqueous matrixes is crucial for safeguarding both human health and the environment. Using sample preparation techniques is essential since these compounds are commonly present in aqueous matrixes at trace levels. Therefore, we aimed to investigate the main analytical methods for the determination of quinolones in aqueous samples based on a systematic literature review. We only considered studies that presented more robust analytical techniques that allowed for more accurate and precise measurement of quinolones/fluoroquinolones in aqueous matrixes. In total, 18 articles met the inclusion criteria and were used in our analysis. A total of 21 quinolone antibacterial agents were investigated in water samples from 13 countries, showing a potential risk around the world. Ciprofloxacin (72.2%), enrofloxacin (61.1%), norfloxacin (50%), marbofloxacin, and levofloxacin (33.3%) were the most frequently evaluated compounds. High-Performance Liquid Chromatography stands out as the predominant instrumental technique for the separation and identification of these compounds. Additionally, among the selected studies in this review, 44% employed liquid-liquid extraction techniques and its miniaturized versions, while 56% opted for solid-phase extraction and its miniaturized variations. Finally, it is important to note that the final method efficiency relies on the entire process, from selecting the instrumental technique with appropriate detection limits, going through the entire sample preparation, to achieving a good recovery through adjustments in the extraction parameters to archive the determination of trace levels.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 1","pages":"19-30"},"PeriodicalIF":4.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs.","authors":"Jihee Lee, Jueun Han, Yejin Song, Boram Gu, Eunjung Kim","doi":"10.1021/acsmeasuresciau.4c00063","DOIUrl":"10.1021/acsmeasuresciau.4c00063","url":null,"abstract":"<p><p>Thermal cycling-based quantitative polymerase chain reaction (qPCR) represents the gold standard method for accurate and sensitive nucleic acid quantification in laboratory settings. However, its reliance on costly thermal cyclers limits the implementation of this technique for rapid point-of-care (POC) diagnostics. To address this, isothermal amplification techniques such as rolling circle amplification (RCA) have been developed, offering a simpler alternative that can operate without the need for sophisticated instrumentation. This study focuses on the development and optimization of toehold-mediated RCA (TRCA), which employs a conformationally switchable dumbbell DNA template for the sensitive and selective detection of cancer-associated miRNAs, specifically miR-21. In addition, we developed variants of hyperbranched TRCA (HTRCA), nicking-assisted TRCA (NTRCA), and hyperbranched NTRCA (HNTRCA) to facilitate exponential amplification by enhancing TRCA through the sequential incorporation of reverse primer (Pr) and nicking endonuclease (nE). By conducting a systematic kinetic analysis of the initial rate and end point signals for varying concentrations of key reaction components, we could identify optimal conditions that markedly enhanced the sensitivity and specificity of the TRCA variants. In particular, HNTRCA, which exploits the synergistic effect of Pr and nE, demonstrated an approximately 3000-fold improvement in the detection limit (260 fM) and a wider dynamic range of more than 4 log orders of magnitude compared to TRCA, thereby evidencing its superior performance. Also, we established a mechanistic model for TRCA that includes the roles of Pr and nE in different amplification processes. Model parameters were fitted to the experimental data, and additional simulations were conducted to compare the four amplification methods. Further tests with real biological samples revealed that this technique showed a good correlation with qPCR in quantifying miR-21 expression in various cell lines (0.9510 of Pearson's <i>r</i>), confirming its potential as a robust and rapid tool for nucleic acid detection. Therefore, the simplicity, high sensitivity, and potential for integration with POC diagnostic platforms make the HNTRCA system suitable for field deployment in resource-limited environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"737-750"},"PeriodicalIF":4.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs","authors":"Jihee Lee,&nbsp;Jueun Han,&nbsp;Yejin Song,&nbsp;Boram Gu* and Eunjung Kim*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0006310.1021/acsmeasuresciau.4c00063","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00063https://doi.org/10.1021/acsmeasuresciau.4c00063","url":null,"abstract":"<p >Thermal cycling-based quantitative polymerase chain reaction (qPCR) represents the gold standard method for accurate and sensitive nucleic acid quantification in laboratory settings. However, its reliance on costly thermal cyclers limits the implementation of this technique for rapid point-of-care (POC) diagnostics. To address this, isothermal amplification techniques such as rolling circle amplification (RCA) have been developed, offering a simpler alternative that can operate without the need for sophisticated instrumentation. This study focuses on the development and optimization of toehold-mediated RCA (TRCA), which employs a conformationally switchable dumbbell DNA template for the sensitive and selective detection of cancer-associated miRNAs, specifically miR-21. In addition, we developed variants of hyperbranched TRCA (HTRCA), nicking-assisted TRCA (NTRCA), and hyperbranched NTRCA (HNTRCA) to facilitate exponential amplification by enhancing TRCA through the sequential incorporation of reverse primer (Pr) and nicking endonuclease (nE). By conducting a systematic kinetic analysis of the initial rate and end point signals for varying concentrations of key reaction components, we could identify optimal conditions that markedly enhanced the sensitivity and specificity of the TRCA variants. In particular, HNTRCA, which exploits the synergistic effect of Pr and nE, demonstrated an approximately 3000-fold improvement in the detection limit (260 fM) and a wider dynamic range of more than 4 log orders of magnitude compared to TRCA, thereby evidencing its superior performance. Also, we established a mechanistic model for TRCA that includes the roles of Pr and nE in different amplification processes. Model parameters were fitted to the experimental data, and additional simulations were conducted to compare the four amplification methods. Further tests with real biological samples revealed that this technique showed a good correlation with qPCR in quantifying miR-21 expression in various cell lines (0.9510 of Pearson’s <i>r</i>), confirming its potential as a robust and rapid tool for nucleic acid detection. Therefore, the simplicity, high sensitivity, and potential for integration with POC diagnostic platforms make the HNTRCA system suitable for field deployment in resource-limited environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"737–750 737–750"},"PeriodicalIF":4.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection.","authors":"Bianca Elizabeth David, Sasya Madhurantakam, Jayanth Babu Karnam, Sriram Muthukumar, Shalini Prasad","doi":"10.1021/acsmeasuresciau.4c00060","DOIUrl":"10.1021/acsmeasuresciau.4c00060","url":null,"abstract":"<p><p>This research aims to develop a portable biosensor device for quickly detecting vWFA2, a biomarker for inflammatory conditions. This sensor could dramatically change detection methods and lead us to improve the sensitivity of our tests to overcome the limitations of conventional detection methods. Our label-free biomolecular assay is constructed on an Au-ZnO electrode surface and uses electrochemical impedance spectroscopy (EIS) to measure the capacitive change in impedance, revealing the binding effects of the target vWFA2, to the capture probe. Our developed biosensor platform exhibits greater sensitivity and specificity, covering a wide dynamic range of 750-24,000 pg/mL and showing a strong correlation with inflammatory conditions. This sensor exhibited a greater accuracy ranging from 86-110% for the known spiked concentrations in nondiluted or modified plasma samples. This electrochemical sensor has the potential to advance point-of-care diagnostic methods due to its high sensitivity and rapid response time. The vision behind this research is to develop an electrochemical sensor that can rapidly and accurately detect disease states, thus creating a pivotal prognostic tool in inflammatory state treatment and ultimately mitigating severe mortality and morbidity.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"721-728"},"PeriodicalIF":4.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection","authors":"Bianca Elizabeth David,&nbsp;Sasya Madhurantakam,&nbsp;Jayanth Babu Karnam,&nbsp;Sriram Muthukumar and Shalini Prasad*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0006010.1021/acsmeasuresciau.4c00060","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00060https://doi.org/10.1021/acsmeasuresciau.4c00060","url":null,"abstract":"<p >This research aims to develop a portable biosensor device for quickly detecting vWFA2, a biomarker for inflammatory conditions. This sensor could dramatically change detection methods and lead us to improve the sensitivity of our tests to overcome the limitations of conventional detection methods. Our label-free biomolecular assay is constructed on an Au-ZnO electrode surface and uses electrochemical impedance spectroscopy (EIS) to measure the capacitive change in impedance, revealing the binding effects of the target vWFA2, to the capture probe. Our developed biosensor platform exhibits greater sensitivity and specificity, covering a wide dynamic range of 750–24,000 pg/mL and showing a strong correlation with inflammatory conditions. This sensor exhibited a greater accuracy ranging from 86–110% for the known spiked concentrations in nondiluted or modified plasma samples. This electrochemical sensor has the potential to advance point-of-care diagnostic methods due to its high sensitivity and rapid response time. The vision behind this research is to develop an electrochemical sensor that can rapidly and accurately detect disease states, thus creating a pivotal prognostic tool in inflammatory state treatment and ultimately mitigating severe mortality and morbidity.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"721–728 721–728"},"PeriodicalIF":4.6,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triazine-Containing Pyridinium Organic Polymer Based on 4-Amino-1,8-naphthalimide Tröger’s Base for Selective Fluorescent Sensing of Organoarsenic Feed Additives in Water","authors":"Ananthu Shanmughan,&nbsp;Karuppaiya Balamurugan,&nbsp;Giriraj Kalaiarasi,&nbsp;Karunya Chitrarasu and Sankarasekaran Shanmugaraju*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0005910.1021/acsmeasuresciau.4c00059","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00059https://doi.org/10.1021/acsmeasuresciau.4c00059","url":null,"abstract":"<p >A new triazine containing pyridinium organic polymer (<b>TB-Py-COP</b>) based on 4-amino-1,8-naphthalimide Tröger’s base is developed and employed as a fluorescent chemosensor for selective sensing of organoarsenic feed additives in water. <b>TB-Py-COP</b> was readily synthesized by reacting “V-shaped” bis-[<i>N</i>-(4-pyridyl)methyl)]-9,18-methano-1,8-naphthalimide-[<i>b,f</i>][1,5]diazocine with cyanuric chloride in DMF at 100 °C for 3 days. <b>TB-Py-COP</b> exhibited a strong fluorescence emission in water and displayed selective, reversible fluorescence sensing responses for the roxarsone feed additive at the nanomolar (19 nM) level of sensitivity. The Stern–Volmer quenching constant (<i>K</i>_SV) was determined to be 1.4 × 10⁴ M^–1. The selective sensing of roxarsone was further demonstrated in competitive environments, lake water, and buffer solutions.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 1","pages":"56–62 56–62"},"PeriodicalIF":4.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triazine-Containing Pyridinium Organic Polymer Based on 4-Amino-1,8-naphthalimide Tröger's Base for Selective Fluorescent Sensing of Organoarsenic Feed Additives in Water.","authors":"Ananthu Shanmughan, Karuppaiya Balamurugan, Giriraj Kalaiarasi, Karunya Chitrarasu, Sankarasekaran Shanmugaraju","doi":"10.1021/acsmeasuresciau.4c00059","DOIUrl":"10.1021/acsmeasuresciau.4c00059","url":null,"abstract":"<p><p>A new triazine containing pyridinium organic polymer (<b>TB-Py-COP</b>) based on 4-amino-1,8-naphthalimide Tröger's base is developed and employed as a fluorescent chemosensor for selective sensing of organoarsenic feed additives in water. <b>TB-Py-COP</b> was readily synthesized by reacting \"V-shaped\" bis-[<i>N</i>-(4-pyridyl)methyl)]-9,18-methano-1,8-naphthalimide-[<i>b,f</i>][1,5]diazocine with cyanuric chloride in DMF at 100 °C for 3 days. <b>TB-Py-COP</b> exhibited a strong fluorescence emission in water and displayed selective, reversible fluorescence sensing responses for the roxarsone feed additive at the nanomolar (19 nM) level of sensitivity. The Stern-Volmer quenching constant (<i>K</i> _SV) was determined to be 1.4 × 10⁴ M^-1. The selective sensing of roxarsone was further demonstrated in competitive environments, lake water, and buffer solutions.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 1","pages":"56-62"},"PeriodicalIF":4.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations for Measurements of Aggregate PFAS Exposure in Precision Environmental Health.","authors":"Katherine E Manz","doi":"10.1021/acsmeasuresciau.4c00052","DOIUrl":"10.1021/acsmeasuresciau.4c00052","url":null,"abstract":"<p><p>Per- and polyfluoroalkyl substances (PFAS) have become a major focus of research due to their widespread environmental presence and adverse health effects associated with human exposure. PFAS include legacy and emerging structures and are characterized by a range of functional groups and carbon-fluorine chains that vary in length (from fewer than 3 carbons to more than 7 carbons). Research has linked PFAS exposure to an array of health concerns, ranging from developmental and reproductive disorders to immune system impairments and an increased risk of certain cancers. In this new era of personalized health, measuring markers of PFAS exposure in human biospecimens is an important part of environmental public health surveillance. PFAS are typically measured in human blood and tissues using targeted approaches, which quantify individual PFAS structures using specific instrumentation. The diversity and complexity of PFAS, the limitations of the targeted approaches due to the sheer number of structures, and the absence of publicly available analytical standards pose significant challenges for measurement methodologies. This perspective aims to describe aggregate PFAS exposure measurements and their potential for use in precision medicine applications including a discussion of the limitations and potential benefits of these aggregate measurements. As public health organizations, healthcare professionals, and the public look for guidance regarding the safe use of and exposure to PFAS, in a pragmatic cost-effective manner, the dynamic field of measurement science is poised to respond with innovative technological solutions to an important public health need.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"620-628"},"PeriodicalIF":4.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations for Measurements of Aggregate PFAS Exposure in Precision Environmental Health","authors":"Katherine E. Manz*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0005210.1021/acsmeasuresciau.4c00052","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00052https://doi.org/10.1021/acsmeasuresciau.4c00052","url":null,"abstract":"<p >Per- and polyfluoroalkyl substances (PFAS) have become a major focus of research due to their widespread environmental presence and adverse health effects associated with human exposure. PFAS include legacy and emerging structures and are characterized by a range of functional groups and carbon–fluorine chains that vary in length (from fewer than 3 carbons to more than 7 carbons). Research has linked PFAS exposure to an array of health concerns, ranging from developmental and reproductive disorders to immune system impairments and an increased risk of certain cancers. In this new era of personalized health, measuring markers of PFAS exposure in human biospecimens is an important part of environmental public health surveillance. PFAS are typically measured in human blood and tissues using targeted approaches, which quantify individual PFAS structures using specific instrumentation. The diversity and complexity of PFAS, the limitations of the targeted approaches due to the sheer number of structures, and the absence of publicly available analytical standards pose significant challenges for measurement methodologies. This perspective aims to describe aggregate PFAS exposure measurements and their potential for use in precision medicine applications including a discussion of the limitations and potential benefits of these aggregate measurements. As public health organizations, healthcare professionals, and the public look for guidance regarding the safe use of and exposure to PFAS, in a pragmatic cost-effective manner, the dynamic field of measurement science is poised to respond with innovative technological solutions to an important public health need.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 6","pages":"620–628 620–628"},"PeriodicalIF":4.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}