ACS Nanoscience Au最新文献_第6页

Assembly of Rolled-Up Collagen Constructs on Porous Alumina Textiles 多孔氧化铝纺织品上卷曲胶原蛋白结构的组装

ACS Nanoscience Au Pub Date : 2023-06-01 DOI: 10.1021/acsnanoscienceau.3c00008

Deepanjalee Dutta, Nina Graupner, Jörg Müssig and Dorothea Brüggemann*,

{"title":"Assembly of Rolled-Up Collagen Constructs on Porous Alumina Textiles","authors":"Deepanjalee Dutta, Nina Graupner, Jörg Müssig and Dorothea Brüggemann*, ","doi":"10.1021/acsnanoscienceau.3c00008","DOIUrl":"10.1021/acsnanoscienceau.3c00008","url":null,"abstract":"Developing new techniques to prepare free-standing tubular scaffolds has always been a challenge in the field of regenerative medicine. Here, we report a new and simple way to prepare free-standing collagen constructs with rolled-up architecture by self-assembling nanofibers on porous alumina (Al2O3) textiles modified with different silanes, carbon or gold. Following self-assembly and cross-linking with glutaraldehyde, collagen nanofibers spontaneously rolled up on the modified Al2O3 textiles and detached. The resulting collagen constructs had an inner diameter of approximately 2 to 4 mm in a rolled-up state and could be easily detached from the underlying textiles. Mechanical testing of wet collagen scaffolds following detachment yielded mean values of 3.5 ± 1.9 MPa for the tensile strength, 41.0 ± 20.8 MPa for the Young’s modulus and 8.1 ± 3.7% for the elongation at break. No roll-up was observed on Al2O3 textiles without any modification, where collagen did not assemble into fibers, either. Blends of collagen and chitosan were also found to roll into fibrous constructs on silanized Al2O3 textiles, while fibrinogen nanofibers or blends of collagen and elastin did not yield such structures. Based on these differences, we hypothesize that textile surface charge and protein charge, in combination with the porous architecture of protein nanofibers and differences in mechanical strain, are key factors in inducing a scaffold roll-up. Further studies are required to develop the observed roll-up effect into a reproducible biofabrication process that can enable the controlled production of free-standing collagen-based tubes for soft tissue engineering.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"286–294"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/8b/ng3c00008.PMC10436369.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10050619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Real-Time Time-Dependent Density Functional Theory Simulations of Plasmonic Nanostructures and Plasmonic Photocatalysis 等离子体纳米结构与等离子体光催化的实时密度泛函模拟研究进展

ACS Nanoscience Au Pub Date : 2023-05-19 DOI: 10.1021/acsnanoscienceau.2c00061

Connor J. Herring, and , Matthew M. Montemore*,

{"title":"Recent Advances in Real-Time Time-Dependent Density Functional Theory Simulations of Plasmonic Nanostructures and Plasmonic Photocatalysis","authors":"Connor J. Herring,  and , Matthew M. Montemore*, ","doi":"10.1021/acsnanoscienceau.2c00061","DOIUrl":"10.1021/acsnanoscienceau.2c00061","url":null,"abstract":"Plasmonic catalysis provides a possible means for driving chemical reactions under relatively mild conditions. Rational design of these systems is impeded by the difficulty in understanding the electron dynamics and their interplay with reactions. Real-time, time-dependent density functional theory (RT-TDDFT) can provide dynamic information on excited states in plasmonic systems, including those relevant to plasmonic catalysis, at time scales and length scales that are otherwise out of reach of many experimental techniques. Here, we discuss previous RT-TDDFT studies of plasmonic systems, focusing on recent work that gains insight into plasmonic catalysis. These studies provide insight into plasmon dynamics, including size effects and the role of specific electronic states. Further, these studies provide significant insight into mechanisms underlying plasmonic catalysis, showing the importance of charge transfer between metal and adsorbate states, as well as local field enhancement, in different systems.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"269–279"},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.2c00061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10050614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity in Cation Exchange Ag+ Doping of CdSe Nanocrystals 阳离子交换Ag+掺杂CdSe纳米晶的非均质性

ACS Nanoscience Au Pub Date : 2023-04-25 DOI: 10.1021/acsnanoscienceau.3c00010

Abigail Freyer, Trevor M. Tumiel, Michelle Smeaton, Benjamin H. Savitzky, Lena F. Kourkoutis and Todd D. Krauss*,

{"title":"Heterogeneity in Cation Exchange Ag+ Doping of CdSe Nanocrystals","authors":"Abigail Freyer, Trevor M. Tumiel, Michelle Smeaton, Benjamin H. Savitzky, Lena F. Kourkoutis and Todd D. Krauss*, ","doi":"10.1021/acsnanoscienceau.3c00010","DOIUrl":"https://doi.org/10.1021/acsnanoscienceau.3c00010","url":null,"abstract":"Cation exchange is becoming extensively used for nanocrystal (NC) doping in order to produce NCs with unique optical and electronic properties. However, despite its ever-increasing use, the relationships between the cation exchange process, its doped NC products, and the resulting NC photophysics are not well characterized. For example, similar doping procedures on NCs with the same chemical compositions have resulted in quite different photophysics. Through a detailed single molecule investigation of a postsynthesis Ag+ doping of CdSe NCs, a number of species were identified within a single doped NC sample, suggesting the differences in the optical properties of the various synthesis methods are due to the varied contributions of each species. Electrostatic force microscopy (EFM), electron energy loss spectroscopy (EELS) mapping, and single molecule photoluminescence (PL) studies were used to identify four possible species resulting from the Ag+-CdSe cation exchange doping process. The heterogeneity of these samples shows the difficulty in controlling a postsynthesis cation exchange method to produce homogeneous samples needed for use in any potential application. Additionally, the heterogeneity in the doped samples demonstrates that significant care must be taken in describing the ensemble or average characteristics of the sample.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"280–285"},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.3c00010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Immune Cell Heterogeneity and Protein Corona on the Cellular Association and Cytotoxicity of Gold Nanoparticles: A Single-Cell-Based, High-Dimensional Mass Cytometry Study 免疫细胞异质性和蛋白冕对金纳米颗粒细胞关联和细胞毒性的影响:一项基于单细胞的高维细胞计数研究

ACS Nanoscience Au Pub Date : 2023-04-24 DOI: 10.1021/acsnanoscienceau.3c00001

Sehee Park, My Kieu Ha, Yangsoon Lee, Jaewoo Song and Tae Hyun Yoon*,

{"title":"Effects of Immune Cell Heterogeneity and Protein Corona on the Cellular Association and Cytotoxicity of Gold Nanoparticles: A Single-Cell-Based, High-Dimensional Mass Cytometry Study","authors":"Sehee Park, My Kieu Ha, Yangsoon Lee, Jaewoo Song and Tae Hyun Yoon*, ","doi":"10.1021/acsnanoscienceau.3c00001","DOIUrl":"10.1021/acsnanoscienceau.3c00001","url":null,"abstract":"Understanding how nanoparticles (NPs) interact with biological systems is important in many biomedical research areas. However, the heterogeneous nature of biological systems, including the existence of numerous cell types and multitudes of key environmental factors, makes these interactions extremely challenging to investigate precisely. Here, using a single-cell-based, high-dimensional mass cytometry approach, we demonstrated that the presence of protein corona has significant influences on the cellular associations and cytotoxicity of gold NPs for human immune cells, and those effects vary significantly with the types of immune cells and their subsets. The altered surface functionality of protein corona reduced the cytotoxicity and cellular association of gold NPs in most cell types (e.g., monocytes, dendritic cells, B cells, natural killer (NK) cells, and T cells) and those immune cells selected different endocytosis pathways such as receptor-mediated endocytosis, phagocytosis, and micropinocytosis. However, even slight alterations in the major cell type (phagocytic cells and non-phagocytic cells) and T cell subsets (e.g., memory and naive T cells) resulted in significant protein corona-dependent variations in their cellular dose of gold NPs. Especially, naive T killer cells exhibited additional heterogeneity than memory T killer cells, with clusters exhibiting distinct cellular association patterns in single-cell contour plots. This multi-parametric analysis of mass cytometry data established a conceptual framework for a more holistic understanding of how the human immune system responds to external stimuli, paving the way for the application of precisely engineered NPs as promising tools of nanomedicine under various clinical settings, including targeted drug delivery and vaccine development.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"323–334"},"PeriodicalIF":0.0,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/88/3c/ng3c00001.PMC10436372.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10050616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-Mediated Electrochemical Synthesis of Manganese Oxide Enhances Its Stability for Water Oxidation 光介导的电化学合成提高氧化锰的水氧化稳定性

ACS Nanoscience Au Pub Date : 2023-04-23 DOI: 10.1021/acsnanoscienceau.3c00002

Chu Qin, Jiang Luo, Dongyan Zhang, Logan Brennan, Shijun Tian, Ashlynn Berry, Brandon M. Campbell and Bryce Sadtler*,

{"title":"Light-Mediated Electrochemical Synthesis of Manganese Oxide Enhances Its Stability for Water Oxidation","authors":"Chu Qin, Jiang Luo, Dongyan Zhang, Logan Brennan, Shijun Tian, Ashlynn Berry, Brandon M. Campbell and Bryce Sadtler*, ","doi":"10.1021/acsnanoscienceau.3c00002","DOIUrl":"10.1021/acsnanoscienceau.3c00002","url":null,"abstract":"New methods are needed to increase the activity and stability of earth-abundant catalysts for electrochemical water splitting to produce hydrogen fuel. Electrodeposition has been previously used to synthesize manganese oxide films with a high degree of disorder and a mixture of oxidation states for Mn, which has led to electrocatalysts with high activity but low stability for the oxygen evolution reaction (OER) at high current densities. In this study, we show that multipotential electrodeposition of manganese oxide under illumination produces nanostructured films with significantly higher stability for the OER compared to films grown under otherwise identical conditions in the dark. Manganese oxide films grown by multipotential deposition under illumination sustain a current density of 10 mA/cm2 at 2.2 V versus reversible hydrogen electrode for 18 h (pH 13). Illumination does not enhance the activity or stability of manganese oxide films grown using a constant potential, and films grown by multipotential deposition in the dark undergo a complete loss of activity within 1 h of electrolysis. Electrochemical and structural characterization indicate that photoexcitation of the films during growth reduces Mn ions and changes the content and structure of intercalated potassium ions and water molecules in between the disordered layers of birnessite-like sheets of MnOx, which stabilizes the nanostructured film during electrocatalysis. These results demonstrate that combining multiple external stimuli (i.e., light and an external potential) can induce structural changes not attainable by either stimulus alone to make earth-abundant catalysts more active and stable for important chemical transformations such as water oxidation.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"310–322"},"PeriodicalIF":0.0,"publicationDate":"2023-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/f4/ng3c00002.PMC10436374.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling the Structure and Morphology of Organic Nanofilaments Using External Stimuli 利用外界刺激控制有机纳米丝的结构和形态

ACS Nanoscience Au Pub Date : 2023-04-18 DOI: 10.1021/acsnanoscienceau.3c00005

Barış Sezgin, Jiao Liu, Diana P. N. Gonçalves, Chenhui Zhu, Tahir Tilki, Marianne E. Prévôt* and Torsten Hegmann*,

{"title":"Controlling the Structure and Morphology of Organic Nanofilaments Using External Stimuli","authors":"Barış Sezgin, Jiao Liu, Diana P. N. Gonçalves, Chenhui Zhu, Tahir Tilki, Marianne E. Prévôt* and Torsten Hegmann*, ","doi":"10.1021/acsnanoscienceau.3c00005","DOIUrl":"10.1021/acsnanoscienceau.3c00005","url":null,"abstract":"In our continuing pursuit to generate, understand, and control the morphology of organic nanofilaments formed by molecules with a bent molecular shape, we here report on two bent-core molecules specifically designed to permit a phase or morphology change upon exposure to an applied electric field or irradiation with UV light. To trigger a response to an applied electric field, conformationally rigid chiral (S,S)-2,3-difluorooctyloxy side chains were introduced, and to cause a response to UV light, an azobenzene core was incorporated into one of the arms of the rigid bent core. The phase behavior as well as structure and morphology of the formed phases and nanofilaments were analyzed using differential scanning calorimetry, cross-polarized optical microscopy, circular dichroism spectropolarimetry, scanning and transmission electron microscopy, UV–vis spectrophotometry, as well as X-ray diffraction experiments. Both bent-core molecules were characterized by the coexistence of two nanoscale morphologies, specifically helical nanofilaments (HNFs) and layered nanocylinders, prior to exposure to an external stimulus and independent of the cooling rate from the isotropic liquid. The application of an electric field triggers the disappearance of crystalline nanofilaments and instead leads to the formation of a tilted smectic liquid crystal phase for the material featuring chiral difluorinated side chains, whereas irradiation with UV light results in the disappearance of the nanocylinders and the sole formation of HNFs for the azobenzene-containing material. Combined results of this experimental study reveal that in addition to controlling the rate of cooling, applied electric fields and UV irradiation can be used to expand the toolkit for structural and morphological control of suitably designed bent-core molecule-based structures at the nanoscale.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 4","pages":"295–309"},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a6/d5/ng3c00005.PMC10436377.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10107023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Label-Free Digital Holotomography Reveals Ibuprofen-Induced Morphological Changes to Red Blood Cells 无标签数字全息断层扫描揭示布洛芬诱导红细胞形态学改变

ACS Nanoscience Au Pub Date : 2023-04-05 DOI: 10.1021/acsnanoscienceau.3c00004

Talia Bergaglio, Shayon Bhattacharya, Damien Thompson* and Peter Niraj Nirmalraj*,

{"title":"Label-Free Digital Holotomography Reveals Ibuprofen-Induced Morphological Changes to Red Blood Cells","authors":"Talia Bergaglio, Shayon Bhattacharya, Damien Thompson* and Peter Niraj Nirmalraj*, ","doi":"10.1021/acsnanoscienceau.3c00004","DOIUrl":"10.1021/acsnanoscienceau.3c00004","url":null,"abstract":"Understanding the dose-dependent effect of over-the-counter drugs on red blood cells (RBCs) is crucial for hematology and digital pathology. Yet, it is challenging to continuously record the real-time, drug-induced shape changes of RBCs in a label-free manner. Here, we demonstrate digital holotomography (DHTM)-enabled real-time, label-free concentration-dependent and time-dependent monitoring of ibuprofen on RBCs from a healthy donor. The RBCs are segmented based on three-dimensional (3D) and four-dimensional (4D) refractive index tomograms, and their morphological and chemical parameters are retrieved with their shapes classified using machine learning. We directly observed the formation and motion of spicules on the RBC membrane when aqueous solutions of ibuprofen were drop-cast on wet blood, creating rough-membraned echinocyte forms. At low concentrations of 0.25–0.50 mM, the ibuprofen-induced morphological change was transient, but at high concentrations (1–3 mM) the spiculated RBC remained over a period of up to 1.5 h. Molecular simulations confirmed that aggregates of ibuprofen molecules at high concentrations significantly disrupted the RBC membrane structural integrity and lipid order but produced negligible effect at low ibuprofen concentrations. Control experiments on the effect of urea, hydrogen peroxide, and aqueous solutions on RBCs showed zero spicule formation. Our work clarifies the dose-dependent chemical effects on RBCs using label-free microscopes that can be deployed for the rapid detection of overdosage of over-the-counter and prescribed drugs.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 3","pages":"241–255"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.3c00004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10073750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complex Dispersion of Detonation Nanodiamond Revealed by Machine Learning Assisted Cryo-TEM and Coarse-Grained Molecular Dynamics Simulations 用机器学习辅助低温透射电镜和粗粒分子动力学模拟揭示爆轰纳米金刚石的复杂弥散

ACS Nanoscience Au Pub Date : 2023-04-05 DOI: 10.1021/acsnanoscienceau.2c00055

Inga C. Kuschnerus, Haotian Wen, Juanfang Ruan, Xinrui Zeng, Chun-Jen Su, U-Ser Jeng, George Opletal, Amanda S. Barnard, Ming Liu, Masahiro Nishikawa and Shery L. Y. Chang*,

{"title":"Complex Dispersion of Detonation Nanodiamond Revealed by Machine Learning Assisted Cryo-TEM and Coarse-Grained Molecular Dynamics Simulations","authors":"Inga C. Kuschnerus, Haotian Wen, Juanfang Ruan, Xinrui Zeng, Chun-Jen Su, U-Ser Jeng, George Opletal, Amanda S. Barnard, Ming Liu, Masahiro Nishikawa and Shery L. Y. Chang*, ","doi":"10.1021/acsnanoscienceau.2c00055","DOIUrl":"10.1021/acsnanoscienceau.2c00055","url":null,"abstract":"Understanding the polydispersity of nanoparticles is crucial for establishing the efficacy and safety of their role as drug delivery carriers in biomedical applications. Detonation nanodiamonds (DNDs), 3–5 nm diamond nanoparticles synthesized through detonation process, have attracted great interest for drug delivery due to their colloidal stability in water and their biocompatibility. More recent studies have challenged the consensus that DNDs are monodispersed after their fabrication, with their aggregate formation poorly understood. Here, we present a novel characterization method of combining machine learning with direct cryo-transmission electron microscopy imaging to characterize the unique colloidal behavior of DNDs. Together with small-angle X-ray scattering and mesoscale simulations we show and explain the clear differences in the aggregation behavior between positively and negatively charged DNDs. Our new method can be applied to other complex particle systems, which builds essential knowledge for the safe implementation of nanoparticles in drug delivery.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 3","pages":"211–221"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/00/5d/ng2c00055.PMC10288606.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9769920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Drug-Eluting Sandwich Hydrogel Lenses Based on Microchamber Film Drug Encapsulation 基于微室膜药物包封的药物洗脱夹层水凝胶透镜

ACS Nanoscience Au Pub Date : 2023-04-05 DOI: 10.1021/acsnanoscienceau.2c00066

Valeriya Kudryavtseva, Mariana Otero, Jiaxin Zhang, Anton Bukatin, David Gould and Gleb B. Sukhorukov*,

引用次数: 2

Recent Advances in Site-Specific Lipid Nanoparticles for mRNA Delivery 用于mRNA递送的位点特异性脂质纳米颗粒的最新进展

ACS Nanoscience Au Pub Date : 2023-03-30 DOI: 10.1021/acsnanoscienceau.2c00062

Xiao Xu, and , Tian Xia*,

{"title":"Recent Advances in Site-Specific Lipid Nanoparticles for mRNA Delivery","authors":"Xiao Xu,  and , Tian Xia*, ","doi":"10.1021/acsnanoscienceau.2c00062","DOIUrl":"10.1021/acsnanoscienceau.2c00062","url":null,"abstract":"The success of mRNA vaccines during the COVID-19 pandemic has greatly accelerated the development of mRNA therapy. mRNA is a negatively charged nucleic acid that serves as a template for protein synthesis in the ribosome. Despite its utility, the instability of mRNA requires suitable carriers for in vivo delivery. Lipid nanoparticles (LNPs) are employed to protect mRNA from degradation and enhance its intracellular delivery. To further optimize the therapeutic efficacy of mRNA, site-specific LNPs have been developed. Through local or systemic administration, these site-specific LNPs can accumulate in specific organs, tissues, or cells, allowing for the intracellular delivery of mRNA to specific cells and enabling the exertion of local or systemic therapeutic effects. This not only improves the efficiency of mRNA therapy but also reduces off-target adverse effects. In this review, we summarize recent site-specific mRNA delivery strategies, including different organ- or tissue-specific LNP after local injection, and organ-specific or cell-specific LNP after intravenous injection. We also provide an outlook on the prospects of mRNA therapy.","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"3 3","pages":"192–203"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.2c00062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9713495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2