Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry最新文献

{"title":"Enzymatic study of Waldeyer’s ring lymphoid tissue: Activity of alkaline and acid phosphatase in palatinal tonsils and adenoids in children with recurrent infection of the ring","authors":"S. Jesic, Ljuba Stojilkovic, V. Djordjević, Z. Petrović, Svetlana Stošić, V. Nesic, Veselinka Novovic, Jasminka Miloradović","doi":"10.2298/JMH0501027J","DOIUrl":"https://doi.org/10.2298/JMH0501027J","url":null,"abstract":"Summary: Activity and kinetic properties of tissue nonspecific alkaline phopsphatase and acid phosphatase were investigated in adenoids and tonsils of 62 children tonsiloadenoidectomised because of recurrent infection using p-nitrophenylphosphate as a substrate. Kinetic properties were calculated by the method of Levenberg-Marquardt. The mean value of tonsil TNAP activity was 3.525 U/mg of protein versus 7.280 U/ mg of protein in adenoids (t = 5.928, df = 60, p < 0.01). ACP activity was also significantly lower in tonsils (10.844 U/mg of protein) than in adenoids (13.059 U/mg of protein) (t = 11.318, df = 60, p < 0.01). There were no influence of age and sex to both enzyme activities. TNAP activity was significantly higher in hypertrophic (4.132 U/mg of protein) than in atrophic (2.531 U/mg of protein) (t = 2.361, df = 20, p < 0.05). Tonsillar TNAP was more effective than adenoid TNAP (t = 11.769, df = 60, p < 0.01). Results suggest the possibility that recurrent infection influences the tonsils more than adenoids and age. Hypertrophy could be an adaptive mechanism of palatinal tonsils during the infection.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133963092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathophysiology and clinical significance of atherogenic lipoprotein phenotype and small dense LDL particles","authors":"Mirjana Djeric","doi":"10.2298/JMH0302101D","DOIUrl":"https://doi.org/10.2298/JMH0302101D","url":null,"abstract":"","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124099405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Otosclerosis: Pathology of the local focus and perilymph","authors":"B. L. Janosevic, V. Djukic, B. Janosevic, V. Nesic, Z. Dudvarski","doi":"10.2298/JMH0304265J","DOIUrl":"https://doi.org/10.2298/JMH0304265J","url":null,"abstract":"Data from numerous pathohistological, histochemical, immunohistochemical and biochemical studies of the otosclerotic tissue in the otic capsule are reviewed and correlated systematically in order to get more complete insight of the local cellular and molecular events in otosclerosis and to derive the basic knowledge from them. The following parameters are focused: sites and distribution of the otosclerotic foci, classification of the local events in otosclerosis, active and inactive otosclerotic foci, osteoid cells, enzyme activity of the otosclerotic foci and perilymph, components of the bone inorganic constituents and matrix. Characteristic morphologic changes and microscopic appearance of the otosclerotic foci are defined as well as the metabolic processes and enzyme disbalances of the disorder with respect to the phase of the otosclerotic bone resorption and formation. The authors specially stress the necessity of constant monitoring and critical evaluation of both the research data and the general concepts in the multifactorial field of otosclerosis.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"284 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121372739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROTEIN MICROARRAYS FOR DIAGNOSIS AND RESEARCH OF ALLERGIC DISEASES","authors":"R. Hiller","doi":"10.2298/JMH0603221H","DOIUrl":"https://doi.org/10.2298/JMH0603221H","url":null,"abstract":"Summary: For both the systematic as well as for the system-wide analysis of protein function, interaction and abundance, protein microarrays represent one of the pillars underlying modern high-throughput Proteomics. The prospect of analysing samples in a highly parallel while miniaturized fashion has fuelled efforts to develop novel diagnostic applications in the cancer, autoimmune or allergy field. Here, I discuss recent advancements in the development of protein microarrays for the profiling of IgE antibodies in the diagnosis of Type 1-related allergic diseases.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128963912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical study of hematological diseases in childhood","authors":"G. Bjelaković, T. Jevtović-Stoimenov, I. Stojanovic, B. Bjelakovic, M. Ilić","doi":"10.2298/JMH0302141B","DOIUrl":"https://doi.org/10.2298/JMH0302141B","url":null,"abstract":"Summary: Hematological diseases in childhood occur frequently. Analysis of hematological status as well as standard biochemical analysis of blood, as a result of hematopoetic system function, are of great importance in detection and recognition of these diseases. All hematological diseases appear as disturbance of hematopoetic system function at the level of the production and viability in blood flow; this disturbance is reflected on the hemogram and biochemical analyses of blood plasma and serum. The basic hematological parameters of blood diseases (except disturbance of blood coagulation) are: Total blood count cells (erythrocytes, leukocytes with differential leukocytes formulae, trombocytes) hemoglobin concentration, amount of hematocrit (PCV, packed cell volume), erythrocytes sedimentation rate, erythrocytes osmotic fragility. Among the standard biochemical analysis the determination of total bilirubin in the calculation fraction of indirect bilirubin, the amount of serum iron and transferrine (TIBC), serum copper and ceruloplasmin are in use in these diseases. Hemoglobin electrophoresis is also use. The measurement of lactate dehydrogenase activity and other enzymological analyses are rarely performed.The basic idea of this report is the evaluation of another biochemical possibility with the aim to understand the nature of hematological disorders by the use a large spectrum of enzyme analyses, which are present in different blood cells and with direct reflection on the serum enzyme activities.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128732919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid disturbances at workers professionally exposed to industrial noise","authors":"J. Jovanović, M. Jovanović","doi":"10.2298/JMH0401069J","DOIUrl":"https://doi.org/10.2298/JMH0401069J","url":null,"abstract":"Industrial noise can damage the hearing organ, cardiovascular, endocrine, gastrointestinal, peripheral and central nervous system of exposed workers. The atherogenic risk at workers professionally exposed to noise was assessed by measuring total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol and calculating the ratio: T-C/HDL-C and LDL-C/HDL-C. The exposed group consisted of 150 workers professionally exposed to noise intensity of 70’110 dB. The results were compared to a control group of 80 workers working in relatively silent environment. Serum lipid parameters were examined by standard methods. All lipid parameters in exposed group were statistically different compared to the control group. All calculated atherogenic ratios were higher for workers in exposed group than the control group. Atherogenic risk raises by the length of exposition to the noise and by the intensity of noise. The most atherogenic risk is presented at workers exposed to noise over the 90 dB intensity.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115160227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary iodine excretion from the 125i-ria laboratory personnel in inep","authors":"R. Nedic, D. Stojanovic, B. S. Savin-Zegarac, S. Cvejic","doi":"10.2298/JMH0201027N","DOIUrl":"https://doi.org/10.2298/JMH0201027N","url":null,"abstract":"The aim of this study was to examine urinary iodine excretion from the 125I-RIA-INEP laboratory personnel. Radiodosimetric measurements have shown that people received ionization energy doses that were significantly bellow the permissible levels, i.e. 0.15 - 0.45 mSv. The amount of iodide in urine varied from 123 to 396 mg/L (1.0 - 3.2 mmol/L) and the mean value was 264 mg/L (2.1 mmol/L; n = 25, SD = 75.3 mg/L or 0.60 mmol/L, CV = 28.5 %). On the basis of the obtained data it can be concluded that professionaly exposed individuals in the examined laboratory consume sufficient amount of dietary iodine, thus minimizing a risk of internal accumulation of 125I.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116289576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategy of IFCC standardization and use of cardiac markers in acute coronary syndromes","authors":"S. Ignjatović","doi":"10.2298/JMH0304303I","DOIUrl":"https://doi.org/10.2298/JMH0304303I","url":null,"abstract":"Although the use of troponin to diagnose acute myocardial infarction (AMI) has been previously proposed, the Committee on Standardization of Markers of Cardiac Damage (C-SMCD) of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) made a recommendation in 1999 to expand on the enzyme diagnostic criteria for AMI to include cardiac-specific proteins. In September 2000, a joint committee of the European Society of Cardiology and the American College of Cardiology (ESC/ACC) published a new definition of AMI that for first time officially included troponin. According to these criteria, as the best biochemical indicator for detecting myocardial necrosis is \"a concentration of cardiac troponin exceeding the decision limit (defined as the 99th percentile of a reference control group) on at least one occasion during the first 24 hours after the onset of clinical event\". The use of creatine kinase MB (CK-MB), measured by mass assays, is still considered as an acceptable alternative only if cardiac troponin assays are not available. It is very important to standardize the clinical use of troponin in diagnosis and management of acute coronary syndromes and to clearly define decision thresholds. Two strategies have competed as the most appropriate for the use of new markers. The first relies on the use of a combination of two markers - a rapid rising marker such as myoglobin, and a marker that takes longer to rise but is more specific, such as cardiac troponin - to enable detection of AMI in patients who present early and late after symptom onset. In the second strategy, only measurement of cardiac troponin is suggested. One of the most important problems in the practical use of the cardiac-specific troponin is the right definition of decision limits. As diagnostic cut-off for clinical use, the IFCC C-SMCD recommends for troponin assays a total imprecision, expressed as coefficient of variation (CV), of <10% at the 99th percentile of a reference control group. For troponin assays that cannot presently meet the 10% CV at the 99th percentile value, a predetermined higher concentration that meets this imprecision goal should be used as cut-off for AMI until the goal of a 10% CV can be achieved at the 99th percentile. It is very important that clinically relevant biomarker, such as cardiac troponin, on which critical decisions will rest, can be measured with highly reliable and standardized methods. There are problems in assay standardization, imprecision interference, and of pre-analytical variability. Cardiac troponin is currently the most sensitive and specific biochemical marker of myocardial damage and is the best marker for diagnosis, risk stratification, and guidance of therapy in acute coronary syndromes.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127396131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting cirrhosis based on standard laboratory parameters in patients with chronic hepatitis C virus infection","authors":"N. Svirtlih, D. Delić, Jasmina Simonović-Babić, N. Maksić, E. Gvozdenović, L. Dokić, I. Boricic, S. Pavić, Dragica Draskovic, Z. Nešić","doi":"10.2298/JMB0603255S","DOIUrl":"https://doi.org/10.2298/JMB0603255S","url":null,"abstract":"Total of 153 patients with chronic Hepatitis C Virus infection was investigated. Patients were classified into three groups: with histologic confirmed liver cirrhosis, clinical advanced cirrhosis, and chronic hepatitis without fibrosis. Activity of aminotransferases (AST, ALT), value of INR and platelet counts were analyzed by SPSS for Windows (version 10.0). Probability of cirrhosis was calculated by HALT-C formula and expressed in percent (http://www.haltctrial.org). Thrombocytopenia was the most important predictive factor for progression/exclusion of cirrhosis. Probability of cirrhosis in patients with histologic cirrhosis, clinical cirrhosis and chronic hepatitis without fibrosis was from 23-93%, 41-98% and 4-65%, respectively. Probability equal/more than 50% had 60%, 90% and 14% of patients from the same groups. In conclusion, liver cirrhosis can be predicted based on standard laboratory parameters in more than half of patients with chronic hepatitis C virus infection. .","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127541943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERUM LIPIDS IN HYPOTHYROID CHILDREN: EFFECT OF DISEASE AND LEVOTHYROXINE REPLACEMENT THERAPY","authors":"V. Radonjic, Z. Jelić-Ivanović, S. Spasić, N. Bogavac-Stanojević, V. Spasojević-Kalimanovska, B. Bojovic","doi":"10.2298/JMH0204331R","DOIUrl":"https://doi.org/10.2298/JMH0204331R","url":null,"abstract":"Summary: Hypothyroidism is associated with dislipidaemia and an increased risk of atherosclerosis. The aim of this study was to examine the effect of disease and long-term levothyroxine replacement therapy on serum lipids in hypothyroid children. We measured concentrations of total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides and thyroid-stimulating hormone in serum samples of 58 children with hypothyroidism (before and after therapy) and in 100 healthy controls. LDL-C and TC values were most markedly affected by the hypothyroid state. The values in the patients were on the average of 100% (LDL-C) and 54% (TC) higher than in the healthy controls. This finding is accordance with the known fact that LDL clearance is slower in hypothyroidism. TG concentrations were slightly higher (p<0.05) and HDL-C lower (p<0.02), but only in some age groups of patients. The effect of long-term L-T4 replacement on LDL-C and TC was quantitatively most pronounced. The values obtained in patients after therapy were markedly lower than before therapy and did not differ significantly from the values found in the corresponding age groups of healthy children. Significantly lower concentrations of HDL-C and TG after L-T4 administration were found only in children aged from 10 to 15 years. When we compared the lipid-lowering effect of L-T4 replacement therapy in normometabolic versus hypometabolic patients, we obtained a significantly greater effect on TC and LDL-C values in the normometabolic than in hypometabolic group (p<0.01). TC and LDL-C concentrations obtained in the L-T4 treated patients correlated well with the corresponding TSH levels (r = 0.770 and 0.725, respectively).","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125385605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}