Chinese Journal of Analytical Chemistry最新文献

{"title":"Integrative systems biology in insomnia: Bridging traditional Chinese medicine and modern science","authors":"Xu Zhang ,&nbsp;Shasha Zhang ,&nbsp;Shanzhong Tan ,&nbsp;Lizhong Guo","doi":"10.1016/j.cjac.2025.100564","DOIUrl":"10.1016/j.cjac.2025.100564","url":null,"abstract":"<div><div>This article is interested in the applications and technological innovations of systems biology to insomnia research within an integrative framework of traditional Chinese medicine (TCM) and modern science. Insomnia, a common sleep disorder, is a significant global health threat and has emerged as an increasing public health concern. Systems biology, based on multi-omics technologies like genomics, proteomics, and metabolomics, enables the explanation of the complex mechanisms of insomnia in an integrative manner. This review considers the possibility of integrating TCM theories with systems biology for identifying new biomarkers and therapeutic targets. The advances such as genome-wide association studies and neurobiological observations are illuminating the pathophysiology of insomnia, which can be integrated with TCM concepts. Network pharmacology and multi-layered regulatory network modeling are highlighted as beneficial in clarifying the pathophysiological mechanisms involved in insomnia. The study emphasizes the importance of personalized medicine and envisions the convergence of TCM and contemporary scientific approaches in the future for better treatment of insomnia.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 9","pages":"Article 100564"},"PeriodicalIF":1.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of Moringa stenopetala seed powder and aluminum sulfate for turbidity and E. coli removal from surface water: The case of bake pond, Borana zone, Ethiopia","authors":"Dereje Diriba CHEMEDA ,&nbsp;Daniel FITAMO","doi":"10.1016/j.cjac.2025.100539","DOIUrl":"10.1016/j.cjac.2025.100539","url":null,"abstract":"<div><div>In rural Borana, Ethiopia, limited access to potable water and the high cost of conventional water treatment methods have led many people to rely on turbid surface water for drinking, exposing them to waterborne diseases. A study was conducted to evaluate the effectiveness of <em>Moringa stenopetala</em> seed powder (MSP) as a natural coagulant for reducing turbidity and <em>Escherichia coli</em> (<em>E. coli</em>) levels in pond water, compared to the conventional coagulant, aluminum sulfate (alum). Water samples were treated with MSP or alum at dosages ranging from 0 to 130 mg/L. The study assessed the effects of initial pH (1.5 to 10.5), settling time (30 to 180 min), and initial turbidity (55 to 319 NTU) on coagulation efficiency for both coagulants. Turbidity, pH, and <em>E. coli</em> levels in the water samples were measured before and after treatment using a portable turbidity meter, portable pH meter, and membrane filtration, respectively. A one-way ANOVA was used to assess significant differences (<em>p</em> &lt; 0.05) between MSP and alum in their coagulation effectiveness. After 120 min of settling, alum (70 mg/L) reduced turbidity from 216 NTU to 1.8 NTU (99.16% removal), while MSP (80 mg/L) reduced turbidity to 4.2 NTU (98.05% removal). Both coagulants achieved turbidity levels below the World Health Organization (WHO) standard of 5 NTU, with MSP showing similar efficacy to alum. In terms of <em>E. coli</em> reduction, alum at 70 mg/L reduced <em>E. coli</em> by 29.78% (from 47 to 33 CFU/100 mL), while MSP at 80 mg/L achieved a 95.74% reduction (to 2 CFU/100 mL). Additionally, MSP did not significantly (<em>P</em> &lt; 0.05) alter the pH of treated water, unlike alum, which typically lowers the pH and requires post-treatment adjustment. These results suggest that MSP is a cost-effective and sustainable alternative to alum, particularly in rural areas like Borana, where access to clean water is limited.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 7","pages":"Article 100539"},"PeriodicalIF":1.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering spectrophotometric analysis of cilnidipine via coupling with amino reagents: application to pharmaceuticals and biological fluids","authors":"Basima A.A. Saleem ,&nbsp;Salim A. Mohammed ,&nbsp;Amer Th. Al-Taee","doi":"10.1016/j.cjac.2025.100563","DOIUrl":"10.1016/j.cjac.2025.100563","url":null,"abstract":"<div><div>Cilnidipine is an important antihypertensive medication within the dihydropyridine class of calcium channel blockers. The precise quantification of cilnidipine concentrations in pharmaceutical formulations and biological fluid samples is crucial for ensuring therapeutic efficacy and safety. Traditional analytical techniques for measuring cilnidipine amounts have included various methods, with oxidative coupling reactions proving particularly effective. This study presents a novel spectrophotometric method for cilnidipine quantification, employing oxidative coupling with 4-aminoAntipyrine and 4-amino diphenylamine. These reactions yield colored compounds that can be detected in the visible spectrum at wavelengths of 528 nm and 721 nm, significantly improving both sensitivity and accuracy. Methods A and B adhere to Beer's law across specified concentration ranges of 1–55 and 1–30 µg/mL, respectively, demonstrating high molar absorptivity of 1.098×10⁴ L/(mol⋅cm) for method A and 2.1179×10⁴ L/(mol⋅cm) for method B, which highlights their analytical robustness. The limit of detections (LOD) was estimation and found to be 0.1159 and 0.3865 µg/mL for methods A and B, correspondingly, while the limit of quantifications (LOQ) was 0.1976 (method A) and 0.5848 µg/mL (method B), showcasing their strong analytical performance. A thorough validation of linearity and precision was performed, with Sandell's sensitivity assessed at 0.04486 µg/cm² for method A and 0.02325 µg/cm² for method B. This innovative approach provides researchers and healthcare professionals with a reliable tool for accurate cilnidipine measurement, thereby enhancing treatment outcomes and ensuring high standards of pharmaceutical quality. The two suggested techniques effectively determined Cilnidipine; with a decent average recovery in pharmaceutical tablets 99.53%–100.2 % and in human urine and serum samples of 99.77%–100.58 %, no intrusions of co-existing additives present in commercial dosage forms were noted.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100563"},"PeriodicalIF":1.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Main active components of Sishen Wan may modulate T cells-related proinflammatory cytokines via alleviating mitochondrial damage caused by oxidative stress in dextran sulphate sodium-induced ulcerative colitis","authors":"Ke LI ,&nbsp;Weiguang LV ,&nbsp;Boning ZHANG ,&nbsp;Shengnan HAN ,&nbsp;Jing HAN ,&nbsp;Yu ZHANG ,&nbsp;Wei WANG ,&nbsp;Weiyu ZANG ,&nbsp;Anqi YANG ,&nbsp;Hongjia WANG ,&nbsp;Chenggang ZHANG","doi":"10.1016/j.cjac.2025.100562","DOIUrl":"10.1016/j.cjac.2025.100562","url":null,"abstract":"<div><h3>Introduction</h3><div>Sishen Wan (SSW), a classical traditional Chinese medicine decoction, is described to treat ulcerative colitis (UC) patients, but the molecular mechanisms of the main active ingredients of SSW on the interaction between mitochondria and T cells are still unclear. This study aimed to determine the main active ingredients of SSW, predict and explore the possible regulatory mechanism of main active ingredients of SSW in modulating mitochondrial function and ameliorating mitochondrial damage, followed by regulating T cell balance during UC development.</div></div><div><h3>Methods</h3><div>Colorimetric test and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the efficacy of SSW on inflammatory injuries of UC and preliminarily explore the mechanisms of SSW against oxidative stress. The main active components and their possible ligands were predicted by network pharmacology, molecular docking, dynamic simulation and three-dimensional-quantitative structure activity relationship (3D-QSAR). RNA-seq analysis and western blot (WB) was conduct to discover the impact of SSW on genetic profile changes, and discover and predict the potentials of anti-mitochondrial damage and proinflammatory T-cells of the selected bioactive compounds.</div></div><div><h3>Results</h3><div>SSW effectively ameliorated the colonic injuries and alleviated the oxidative stress in the dextran sulphate sodium (DSS)-induced UC. Angelicin, corylifolinin, psoralen and rutaecarpine, derived from SSW, were identified as the main components of SSW, and might interact with CYP2C9 and CYP1A1 due to the lowest binding energy. SSW alleviated UC via regulating genes related to mitochondrial function and T cell responses based on RNA-seq data. Cytc-related targets and T cell-associated proinflammatory cytokines were downregulated, while mtDNA repairing-related targets were upregulated with SSW intervention. Moreover, the caspase, inflammasome and Th1 and Th17 polarizing-related genes are positively correlated with cytochrome C oxidase (COX), caspase and inflammasome-associated genes, respectively.</div></div><div><h3>Conclusion</h3><div>Taken together, this study not only identifies the main bioactive ingredients of SSW and their possible ligands, but also provides angelicin, corylifolinin, psoralen and rutaecarpine may alleviate oxidative stress and mitochondrial damage, followed by modulating Th1 and Th17-related proinflammatory cytokines.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 9","pages":"Article 100562"},"PeriodicalIF":1.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly new kinetic spectrophotometric method for analysis of azithromycin in dosage forms and spiked human plasma","authors":"Safwan Ashour","doi":"10.1016/j.cjac.2025.100561","DOIUrl":"10.1016/j.cjac.2025.100561","url":null,"abstract":"<div><div>A new kinetic technique to quantify azithromycin in dosage forms and plasma was interesting through the reaction of azithromycin dihydrate (AZT) with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium. The formed stable product was followed by measuring the increased absorbance with time at 452 nm. Four kinetic procedures were applied for the determination of AZT, the initial rate and fixed time (at 20 min) methods are the most suitable for plotting the calibration graphs in the concentration ranges 1.50–30.00 and 1.50–33.00 μg/mL with detection limits of 0.023 and 0.018 μg/mL, respectively. The <em>E</em>_a, Δ<em>H</em>^‡, Δ<em>S</em>^‡, and Δ<em>G</em>^‡ are evaluated for the reaction and found to be 8.177 kJ/mol, 5.742 kJ/mol, –198.34 J/K mole, and 64.846 kJ/mol, respectively. The suggested kinetic methods were applied to determine AZT in marketed formulations and spiked human plasma, and were found to be more sustainable, eco-friendly, efficient, and practicable than the reported BP method, by applying green and white tools; AES, AGREE, AGREEprep, GAPI, and RGB, making it a safer alternative to be considered.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100561"},"PeriodicalIF":1.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Mechanism of Ligustri Lucidi Fructus in Treating Liver and Kidney Yin Deficiency Based on Network Pharmacology","authors":"Zhimin CHEN ,&nbsp;Yunxiu JIANG ,&nbsp;Mayijie CAO ,&nbsp;Ye ZHOU ,&nbsp;Xiaoli ZHU ,&nbsp;Zhuolin JIA ,&nbsp;Jie WU ,&nbsp;Lingying YU ,&nbsp;Changjiang HU","doi":"10.1016/j.cjac.2025.100554","DOIUrl":"10.1016/j.cjac.2025.100554","url":null,"abstract":"<div><h3>Background</h3><div>Traditional Chinese medicine (TCM) syndrome, also known as ZHENG, represents a fundamental concept in TCM. It summarizes the pathological changes of a disease at specific stages of its development. Liver and kidney Yin deficiency syndrome (LKYDS), one of a pathologic and diagnostic pattern caused by the imbalance of Yin and Yang. <em>Ligustri Lucidi Fructus</em> (LLF) is the dried mature fruit of <em>Ligustrum lucidum</em> Ait. It has the effects of nourishing the liver and kidneys. To screen the active ingredients of LLF for treating liver and kidney yin deficiency based on network pharmacology and to explore their potential targets and mechanisms.</div></div><div><h3>Methods</h3><div>We obtained disease-related targets through GeneCards and DisGeNET databases, and utilized Venny 2.1.0 to obtain targets at the intersection of components and diseases. Protein-protein interaction (PPI) analysis and GO function and KEGG pathway enrichment analysis were performed on the potential targets with the help of STRING and DAVID databases, respectively. To clarify the biological process and pathway information, which were further visualized by using the microbiology platform. 120 male SD rats were randomly divided into a blank group, a model group, a raw product group, a processed product group, and a positive group for <em>in vivo</em> experiments. A liver and kidney yin deficiency model induced by levothyroxine sodium was established and continuously administered for 14 days. After the last administration, measure the thymus index, as well as the levels of ALT, AST, ALP, TP, ALB, CREA, UREA, and UA.</div></div><div><h3>Results</h3><div>LLF contains to 63 active components, 511 component targets, 1467 liver-related disease targets, 164 potential targets, 3571 kidney-related disease targets, 292 potential targets. GO functional enrichment involves negative regulation of protein phosphorylation, response to xenobiotic stimuli, apoptotic process, etc. KEGG signaling pathway involves lipids and atherosclerosis, PI3K-Akt signaling pathway and so on. Compared with the blank group, the model group rats showed weight loss, significant thymus atrophy, and a significant decrease in thymus index. The levels of ALT, AST, ALP, CREA, UREA, UA, TP and cGMP were significantly increased, while the levels of cAMP, ALB and cAMP/cGMP were significantly decreased. Compared with the model group, both the positive group and each treatment group showed an increase in thymus index, which significantly reduced the levels of ALT, AST, ALP, CREA, UREA, UA, TP and cGMP in the serum of rats, and significantly increased the levels of cAMP, ALB and cAMP/cGMP. Moreover, there were certain differences between the processed and raw products of LLF. Compared with the raw group, processed LLF showed better effects in ALT, ALB, UREA, TP, cAMP, cAMP/cGMP and other aspects.</div></div><div><h3>Conclusions</h3><div>The main active ingredients in LLF may participate in","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100554"},"PeriodicalIF":1.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidate molecular mechanisms of 173 compounds for potential breast cancer therapeutics: Insights through integrating network pharmacology, molecular docking and molecular dynamics simulation","authors":"Sanjeevi PANDIYAN ,&nbsp;Tiantian RUAN ,&nbsp;Zhuheng ZHONG ,&nbsp;Min YAO ,&nbsp;Li WANG","doi":"10.1016/j.cjac.2025.100557","DOIUrl":"10.1016/j.cjac.2025.100557","url":null,"abstract":"<div><div>All over the world, breast cancer is one of the most common cancers in women and is identified as the prevalent cause of death. Hence, the urgency of developing novel anti-breast cancer drugs for combating this deadly disease with potential efficiency is associated with current therapeutics. To address this issue, in our present work we collected recently analyzed 173 compounds from the scientific literature as much information as possible during 2021–2024 for the first time to elucidate the underlying molecular mechanisms associated with breast cancer via comprehensive analysis that integrates network pharmacology, molecular docking, molecular dynamics, and Molecular Mechanics with Generalized Born and Surface Area solvation (MM/GBSA). Molecular properties and drug-likeness were screened for obtained compounds to probe into the mechanism of action. The compound-target network, protein-protein interaction (PPI) network, Gene Ontology (GO) functional enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed with the aim of analyzing molecular mechanisms associated with breast cancer. Afterward, 12 potentially active compounds were carefully identified along with 192 common targets, including 8 pertinent core targets such as PIK3R1, PIK3CB, PIK3CA, PIK3CD, AKT1, AKT2, AKT3, and PTPN11. Molecular docking simulations revealed a robust score between AKT1-Capivasertib, PTPN11-Olaparib, PIK3R1-(1S)-2-(4-phenylmethoxyphenyl)-<em>N-</em>(pyridin-2-ylmethyl)cyclopropan-1-amine, PIK3CA-(1S)-2-(4-phenylmethoxyphenyl)-<em>N-</em>(pyridin-2-ylmethyl)cyclopropan-1-amine, PIK3CB-Capivasertib, AKT2-Ibuprofen Sodium, PIK3CD-Capivasertib and AKT3-<em>N-</em>(2-Hydroxyphenyl)-2-propylpentanamide complexes with strong binding interactions of 9.2353, 9.2016, 8.7742, 7.8234, 7.7083, 7.6387, 7.3778 and 6.6705, respectively. The key findings of outcome are corroborated by molecular dynamics simulation at 300 K for 200 ns to reinforce intermolecular mechanism between pertinent core targets and potential active compounds. In addition, overall free binding energy is calculated for eight complexes employing MM/GBSA, and the results indicate that Capivasertib has energetically favourable binding towards PIK3CD with binding free energy of −41.14 kcal/mol. Finally, the light of these results provides new insights into understanding the mechanism of action, including compounds, targets, potent biological processes, cellular components, molecular functions, and pathways involved that may represent an essential part of current breast cancer therapeutics.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 9","pages":"Article 100557"},"PeriodicalIF":1.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raman imaging-chemometrics analysis of cell wall biopolymer dynamics in Pseudomonas syringae pv. actinidiae-infeicted kiwifruit stems","authors":"Wei TIAN ,&nbsp;Longhui LUO ,&nbsp;Bingce WANG ,&nbsp;Zhibo ZHAO ,&nbsp;Chao KANG ,&nbsp;Dongmei CHEN ,&nbsp;Xiufang YAN ,&nbsp;Youhua LONG","doi":"10.1016/j.cjac.2025.100556","DOIUrl":"10.1016/j.cjac.2025.100556","url":null,"abstract":"<div><div><em>Pseudomonas syringae</em> pv. <em>actinidiae</em> (<em>Psa</em>), the pathogen that causes bacterial canker disease in kiwifruit, has brought about substantial losses to the kiwifruit industry. Investigating the infection process at both the cellular and microscopic levels is of great significance for the formulation of effective control strategies against this disease. Thus, the establishment of appropriate chemical imaging analysis methods becomes essential. Confocal Raman microspectral imaging (CRMI), combined with chemometrics, provides an intuitive means to visualize and characterize the spatiotemporal changes of biopolymers in the cell walls of both healthy and infected kiwifruit stems. Raman spectra of different infected stems exhibit clustering effects in principal component analysis (PCA), and a classification model constructed using support vector machines (SVM) achieves an accuracy of 97 %. Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) is utilized to resolve spectral matrices and concentration profiles from raw Raman imaging signals. The reconstructed concentration data yields accurate molecular imaging maps of high-methylated pectin (HMP), low-methylated pectin (LMP), cellulose, hemicellulose, and lignin. The results indicate that, three days after <em>Psa</em> infection, the content of cellulose and HMP in the cell wall increases, while the changes in hemicellulose, lignin, and LMP are minimal. However, five days after infection, the contents of HMP, LMP, cellulose, hemicellulose, and lignin decrease significantly, resulting in the disruption of the cell-wall structure. The chemical imaging method proposed in this study shows great promise as an effective means for studying the bacterial infection process in kiwifruit stems at the cellular level.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100556"},"PeriodicalIF":1.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the mechanism of Huaier on psoriasis based on transcriptome sequencing analysis","authors":"Feng ZHANG,&nbsp;Dongqiang SU","doi":"10.1016/j.cjac.2025.100555","DOIUrl":"10.1016/j.cjac.2025.100555","url":null,"abstract":"<div><div>Psoriasis is an inflammatory immune skin disease with high incidence worldwide. As a traditional Chinese medicine (TCM), Huaier has a variety of pharmacological effects such as immune regulation and anti-inflammation. The aim of this study is to investigate the mechanism of action of Huaier on psoriasis and provide direction and basis for the treatment of psoriasis. Human keratinocyte (HaCaT) cells are frequently investigated as a target for psoriasis. Previous studies have found that Huaier can inhibit the proliferation and activity of HaCaT cells, and arrest the cell cycle of HaCaT cells in G1 phase, but the mechanism is still unclear. The aim of this study was to elucidate the role of Huaier in inhibiting the proliferation of HaCaT cells and to analyze its potential targets and molecular mechanisms. At different concentrations of Huaier, compared with the control group, Huaier significantly inhibited the proliferation and activity of HaCaT cells in a concentration-dependent manner (<em>p</em> &lt; 0.05). The results of transcriptome sequencing analysis after Huaier treatment at IC50 concentration showed that Huaier significantly changed the expression of 103 genes in HaCaT cells and regulated 15 signaling pathways (<em>p</em> &lt; 0.05). The results of correlation analysis showed that Huaier may regulate the IL-17 signaling pathway, which is important in the pathogenesis of psoriasis, by affecting the expression of key genes such as IL-6, ARRB1, CDH5, MYH11 and PPP3R1, and inhibit the activity of HaCaT cells, thus exerting a potential therapeutic effect on psoriasis.This will provide clues and directions for the treatment of psoriasis with TCM.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100555"},"PeriodicalIF":1.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profiles changes in Belamcanda chinensis: A metabolomic analysis of crude and processed samples","authors":"Yumiao HUANG ,&nbsp;Shuna JIN ,&nbsp;Rongzeng HUANG ,&nbsp;Zhaoxiang ZENG ,&nbsp;Zecai ZHAN ,&nbsp;Hao YUAN ,&nbsp;Dongru LI ,&nbsp;Meizhu DAI ,&nbsp;Wenjie WANG ,&nbsp;Cheng CHEN","doi":"10.1016/j.cjac.2025.100559","DOIUrl":"10.1016/j.cjac.2025.100559","url":null,"abstract":"<div><div><em>Belamcanda chinensis</em> (BC) is a traditional Chinese herbal medicine with a long history of use. However, there are few studies investigating the variations in chemical composition between unprocessed and processed BC. This study aimed to identify the differential compounds present after the processing of BC and to establish a scientific basis for optimizing its processing technology and quality control. In this study, three types of processed BC products were prepared based on relevant literature and historical texts: water extract of rice-processed (RB), leaves of <em>Phyllostachys bambusoides Sieb. et Zucc</em>-processed (PB), and a combination of water extract of rice with leaves of <em>Phyllostachys bambusoides Sieb. et Zucc</em>-processed (RPB). Ultra performance liquid chromatography (UPLC) fingerprint analysis was performed on an Agilent 1290 liquid chromatograph to evaluate the similarity of the 10 principal flavonoids among different BC samples. Additionally, an untargeted metabolomics approach using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was utilized to identify differential metabolites. Fingerprint analysis showed no significant differences in the 10 main flavonoids between unprocessed and processed samples. Untargeted metabolomics identified 85 differential metabolites, with 57, 60, and 63 metabolites detected in the RB, PB, and RPB processed groups, respectively. Compared with raw BC, the RB group showed increased levels of 16 metabolites and decreased levels of 41; the PB group exhibited 11 upregulated and 49 downregulated metabolites; while the RPB group demonstrated 15 elevated and 48 reduced metabolites. Among these processed groups, PB and RPB exhibited more similar metabolic profiles compared to RB. These findings represent the first systematic investigation into the chemical composition of processed BC, thereby addressing a critical gap in the existing research. Furthermore, they establish a scientific foundation for optimizing processing techniques and enhancing quality control, while also contributing to the modernization and reinterpretation of traditional processing theories.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100559"},"PeriodicalIF":1.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}