Chinese Journal of Analytical Chemistry最新文献

{"title":"An ultrasensitive strip sensor for rapid detection of African swine fever virus","authors":"Mengjing ZHANG,&nbsp;Lingling GUO,&nbsp;Xinxin XU,&nbsp;Hua KUANG,&nbsp;Chuanlai XU,&nbsp;Liqiang LIU","doi":"10.1016/j.cjac.2024.100416","DOIUrl":"10.1016/j.cjac.2024.100416","url":null,"abstract":"<div><p>African swine fever (ASF) is a highly contagious disease caused by the African swine fever virus (ASFV) infecting pigs, which has caused huge economic losses in countries around the world. Currently, there is no effective vaccine, and the prevention and control of ASF is mainly through rapid detection, so it is particularly important to carry identify and develop rapid detection methods for ASFV. In this study, recombinant plasmid PET-28a(+)-p30 was constructed, and the recombinant protein was obtained by inducing expression and Ni⁺ resin affinity column purification. Mice were immunized with recombinant p30 protein, and after three immunizations, ten strains of hybridoma cells that stably secreted monoclonal antibodies (mAbs) against p30 protein were obtained by cell fusion and subcloning. A colloidal gold immunochromatography assay (GICA) based on double antibody sandwich technology was established to screen the paired antibodies, and the trapping and detecting antibodies were mAb-11F11 and mAb-7A8, respectively, with a detection limit of 1 ng/mL, which laid an important material foundation for the early detection of ASF in the future.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100416"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000616/pdfft?md5=8fed3b942e38d115d709b37bdc5d58a6&pid=1-s2.0-S1872204024000616-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State of the Art: Natural fibre-reinforced composites in advanced development and their physical/chemical/mechanical properties","authors":"Krishnasamy KARTHIK ,&nbsp;Ramesh kumar RAJAMANIKKAM ,&nbsp;Elumalai Perumal VENKATESAN ,&nbsp;Sunil BISHWAKARMA ,&nbsp;Ravi KRISHNAIAH ,&nbsp;C Ahamed SALEEL ,&nbsp;Manzoore Elahi M. SOUDAGAR ,&nbsp;M.A. KALAM ,&nbsp;Muhammad Mahmood ALI ,&nbsp;Muhammad Nasir BASHIR","doi":"10.1016/j.cjac.2024.100415","DOIUrl":"10.1016/j.cjac.2024.100415","url":null,"abstract":"<div><p>Nowadays, people are highly conscious of the environment, leading to rapid growth and progress in research and innovation in eco-friendly natural fiber composites (NFCs), which are also cost-effective. The sustainable development of biodegradable NFCs obtained from renewable sources is paving the way for the replacement of synthetic fiber composites. Furthermore, researchers are focusing on enhancing the mechanical performance of NFCs for various applications. Some renewable sources, such as rice, wheat, and corn, have been used to produce polylactic acid-based NFCs. Due to their low greenhouse gas emissions and minimal energy consumption during production, NFCs are also suitable for 3D printing applications. However, they do have some drawbacks, and to overcome them, they are often blended with other NFCs. The purpose of this review article is to provide data on the physical, chemical, and mechanical properties of natural fiber-reinforced composites to assist new researchers. The central idea is that natural, biodegradable, and eco-friendly materials can replace plastics, enhancing their properties in terms of mechanical strength, thermal resistance, antimicrobial properties, water absorption, and degradability. The use of NFCs is flourishing in various fields. Every year, a substantial amount of agricultural waste with potential fibers is discarded. Some examples of waste products include fruit, including areca and tamarind. The reinforcement obtained from these materials is utilized in polymer composites for lightweight structures, the automotive industry, and domestic applications.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100415"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000604/pdfft?md5=410b5bc0056a9146019c4cdcf6bfd8bc&pid=1-s2.0-S1872204024000604-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the effect of processing methods on ginseng quality and key technologies for improvement","authors":"Yong-Fei QIAO ,&nbsp;Rong-Can WANG ,&nbsp;Xin-Yi WANG ,&nbsp;Ya-Li LI","doi":"10.1016/j.cjac.2024.100413","DOIUrl":"https://doi.org/10.1016/j.cjac.2024.100413","url":null,"abstract":"<div><p>As an important medicinal plant, ginseng has various pharmacological activities such as antioxidant, anti-tumor, and anti-inflammatory. The magnitude of these pharmacological activities is closely related to ginseng quality, so to improve the pharmacological activities of ginseng, it is first necessary to improve its quality. In addition to ginseng variety, cultivation, origin, harvest time, and other factors, the impact of processing on ginseng quality is also crucial. This article reviews the different methods of ginseng processing and their impact on ginseng quality, and proposes some strategies to improve ginseng quality through processing methods, aiming to provide technological support for the high-quality development of the ginseng industry.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100413"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000586/pdfft?md5=5f905495bc7400adba238f648887f7eb&pid=1-s2.0-S1872204024000586-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of activated carbon/alginate for the concurrent removal efficiency of paracetamol and caffeine from wastewater in their binary solutions","authors":"Samar M. Mahgoub ,&nbsp;Amna A. Kotp ,&nbsp;Mahmoud A. Mohamed ,&nbsp;Haifa E. Alfassam ,&nbsp;Hassan A. Rudayni ,&nbsp;Ahmed A. Allam ,&nbsp;Rehab Mahmoud ,&nbsp;S.A. Abdel Moaty","doi":"10.1016/j.cjac.2024.100403","DOIUrl":"10.1016/j.cjac.2024.100403","url":null,"abstract":"<div><p>The increasing amount of pharmaceutical chemicals in wastewater is a concern for both public health and the environment. This research investigates the effectiveness of a new composite material made of alginate-activated carbon in removing caffeine and paracetamol from wastewater simultaneously. The composite material, produced using a simple technique, demonstrates excellent removal rates and exceptional adsorption properties for both medicinal chemicals. The structural stability and integrity of the composite are confirmed through comprehensive characterization methods including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction, energy dispersive X-ray (EDX), and BET surface area analysis. The study uses batch adsorption experiments to assess the impact of various factors, such as pH, initial concentration, and contact time, on the efficiency of caffeine and paracetamol removal. The highest values for the adsorption process were 89.63% for Paracetamol and 97.32% for Caffeine, achieved under optimal experimental conditions (pH 7, 0.1 g dose of adsorbent at elevated temperature). Additionally, Kinetic studies and thermodynamic parameters such as Δ<em>S</em>°, Δ<em>G</em>º, and Δ<em>H</em>° were calculated, indicating that the adsorption process was endothermic and spontaneous for paracetamol, and exothermic and non-spontaneous for caffeine. Seven non-linear equilibrium isotherm models were utilized to fit the experimental data for paracetamol and caffeine at pH 7 showing maximum adsorption capacities (<span><math><msub><mi>q</mi><mi>max</mi></msub></math></span>) of 606.80 and 725.05 mg/g for Paracetamol and Caffeine, respectively with a high regression coefficient (<em>R</em>²) of 0.99 using alginate-activated carbon as an adsorbent, The results demonstrate the potential of alginate-activated carbon as an effective adsorbent for the simultaneous removal of various pharmaceutical pollutants, highlighting the synergistic adsorption effects of the composite material. The study also examines the safety of the composite material, particularly in the context of potential medical applications. The study emphasizes the potential for sustainable and repeated use of the activated carbon/alginate composite, demonstrating that it maintains both its structural integrity and adsorption effectiveness after multiple cycles of use. This study evaluated the suitability of the suggested analytical method using BAGI, a metric with a unique formula. The BAGI is a complementary tool to GAPI, Complex GAPI, AGREE, AGREE prep, and ESA. A major focus is on the practical elements of white analytical chemistry centered around \"blue”.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100403"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000483/pdfft?md5=847b086ff884c88e3392d14d887dcfd0&pid=1-s2.0-S1872204024000483-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study on the mechanism of Guasha in treating Parkinson's disease based on molecular level and ultra-trace proteomics analysis","authors":"Qiuqin WANG ,&nbsp;Li ZHANG ,&nbsp;Heng WENG ,&nbsp;Qing WANG ,&nbsp;Penglu CHEN ,&nbsp;Hua CHEN ,&nbsp;Rongrong JIANG ,&nbsp;Guihua XU ,&nbsp;Yamei BAI ,&nbsp;Xi CHEN","doi":"10.1016/j.cjac.2024.100402","DOIUrl":"10.1016/j.cjac.2024.100402","url":null,"abstract":"<div><p>Guasha is a widely applied non-pharmacological therapy of traditional Chinese medicine (TCM), which has been proved to be effective in the treatment of Parkinson's disease (PD). The objective of this study was to explore the mechanisms of Guasha therapy in the pathological processes including neuroinflammation in PD. After 3 Guasha courses, a reduction in α-synuclein aggregation and alleviation of microglia activation were observed in the substantia nigra pars compacta (SNpc). Furthermore, levels of TNF-α, IL-1β and IFN-γ in the SNpc decreased, while plasma TGF-β, IL-4 and IL-10 increased. Moreover, both TH protein and mRNA levels in the SNpc, as well as dopamine levels in the striatum, exhibited an increase. The proteomics analysis results based on plasma-derived exosomes revealed a total of 943 differentially expressed proteins identified. Compared to the model group, the Guasha group screened for 82 differential proteins, with 30 upregulated and 52 downregulated. The improvement of pathological changes in the PD model mice treated with Guasha primarily involves biological processes such as oxidative stress, immune response and inflammation, and cellular structure regulation. It involves signaling pathways related to aldosterone synthesis and secretion, adrenergic signaling in myocardial cells, COVID-19-related inflammatory signaling, and neurotrophic signaling. Collectively, the mechanisms of Guasha for treating PD might be closely related to inhibiting microglial cell activation-mediated neuroinflammation, regulating oxidative stress, cellular structure, aldosterone synthesis and secretion-mediated electrolyte balance, as well as noradrenergic signaling-mediated neuroprotection. These findings provided new insight for Guasha in treating PD and would potentially enhance therapeutic interventions.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100402"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000471/pdfft?md5=7d50d14248abd8f3a4ed8fdc0808e940&pid=1-s2.0-S1872204024000471-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141136710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer and phosphorothioated DNA engineered liposomes as a targeted intracellular protein delivery system","authors":"Jiamei LI ,&nbsp;An YAN ,&nbsp;Mingquan WANG ,&nbsp;Di LI","doi":"10.1016/j.cjac.2024.100400","DOIUrl":"10.1016/j.cjac.2024.100400","url":null,"abstract":"<div><p>Targeted delivery of proteins into desired cell groups is crucial for disease therapy and cellular functionalization. However, current delivery methods face severe side effects and off-target problems, making it difficult to achieve cell-targeted protein delivery. Herein, we developed a user-friendly membrane fusion liposome for cancer cell-targeted protein delivery. Phosphorothioated DNA-mediated membrane fusion was employed as an efficient transmembrane delivery approach. The phosphorothioated DNA was capped with the AS1411 aptamer, which specifically recognizes nucleolar proteins on the cancer cell membrane, enabling a controllable delivery of proteins into targeted cancer cells. This delivery system exhibited commendable biocompatibility and targeted delivery ability, thereby realizing highly effective cancer cell inhibition in vitro. The in vivo results further suggested that the membrane protein-responsive membrane fusion delivery system offers a new avenue for highly biocompatible and targeted protein delivery.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100400"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000458/pdfft?md5=2126a0b8837b2a288978dfa8007829ad&pid=1-s2.0-S1872204024000458-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on H2 sensors based on FET","authors":"Guochao Yan,&nbsp;Siguo Xiao","doi":"10.1016/j.cjac.2024.100401","DOIUrl":"https://doi.org/10.1016/j.cjac.2024.100401","url":null,"abstract":"<div><p>Highly selective, sensitive, and fast hydrogen sensing technology is becoming increasingly important in the processes of production, transportation, and usage of hydrogen energy. Field-effect transistor (FET) is the basic element of modern IC. When serving as a gas sensor, FET poses advantages of small size, high sensitivity, and low power consumption. This article reviews the latest developments in FET hydrogen sensors based on channel materials from traditional silicon, III-V compound semiconductors to novel channel materials carbon nanotubes, graphene, and two-dimensional black phosphorus. Firstly, the structure of FET sensors was investigated. Then the sensitive materials severing as gate were reviewed and efforts to improve the performance was summarized. Then, we discuss the sensitive materials that are currently available, with a focus on the interaction mechanisms between hydrogen and the sensitive materials. Lastly, methods to enhance sensor performance by modifying the physical and chemical properties of the sensitive materials are presented. Finally, the article provides an outlook on the future development of FET type hydrogen gas sensing.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100401"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S187220402400046X/pdfft?md5=8d54383c5151d78b666045c35bb107c7&pid=1-s2.0-S187220402400046X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141324887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorescent aptasensor based on functional graphene oxide and FRET strategy simultaneously detects aflatoxins B1 and aflatoxins M1","authors":"Yuzheng Cai,&nbsp;Ge Guo,&nbsp;Yankun Fu,&nbsp;Xianqing Huang,&nbsp;Tianlin Wang,&nbsp;Tiange Li","doi":"10.1016/j.cjac.2024.100408","DOIUrl":"10.1016/j.cjac.2024.100408","url":null,"abstract":"<div><p>Simultaneous and rapid detection of various mycotoxins in food holds significant practical importance in the field of food processing and safety. In this study, a fluorescent aptasensor based on functionalized graphene oxide (FGO) is developed for simultaneous detection of aflatoxin B₁ (AFB₁) and aflatoxin M₁ (AFM₁). The two aptamers specific to AFB₁ and AFM₁ are labeled with Cy3 and Cy5 respectively. Both the aptamers can be adsorbed onto the surface of FGO through π-π stacking, resulting in fluorescence resonance energy transfer (FRET) between the fluorophore and FGO. The absence of target leads to quenching of fluorescence while presence of either aflatoxin causes interaction between corresponding aptamer and target, leading to release from FGO surface thereby turning on fluorescence signal. The limit of detection (LOD) for AFB₁ is determined as 8.7 pg/mL whereas for AFM₁ it is found to be 20.1 pg/mL, demonstrating fast and sensitive detection capability using this approach. Furthermore, the aptasensor exhibits good specificity and selectivity even under influence from other common interfering toxins. With its simplicity in operation and portability features, this sensor has potential applications for establishing sensitive and portable on-site detection methods for various mycotoxins.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100408"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000537/pdfft?md5=2d29d4f9039fc888c29e6a3966d51d64&pid=1-s2.0-S1872204024000537-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of small molecule compounds bound to proteins","authors":"Haoran XU,&nbsp;Hui WANG,&nbsp;Yuanjing ZHAO,&nbsp;Weiquan ZHAN,&nbsp;Simin CHEN,&nbsp;Ping WANG","doi":"10.1016/j.cjac.2024.100398","DOIUrl":"10.1016/j.cjac.2024.100398","url":null,"abstract":"<div><p>Protein-small molecule interactions play an important role in the life activities of organisms. Since protein/small molecule interactions are important for elucidating the diverse uses and their mechanisms in the fields of biological life multiplicity, disease cure, drug development, etc., methods to accurately detect protein/small molecule interactions have attracted much attention. This paper reviews the recent advances in interaction detection methods based on optical, thermodynamic, chromatographic and other principles. The structural types of small molecules for which the methods are mainly suitable and the principles of method selection are discussed, and the prospects for the combined application of different methods in the field of complex small molecule drugs are also envisaged.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100398"},"PeriodicalIF":1.2,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000434/pdfft?md5=f99a30b9ada1da8a4ae68ff3bbff9a88&pid=1-s2.0-S1872204024000434-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active substances and molecular mechanisms of Astragali radix and Angelicae Sinensis radix against idiopathic pulmonary fibrosis effects by network pharmacology and in vitro experiments","authors":"Haolin CHU ,&nbsp;Shanshan LIU ,&nbsp;Shujing ZHANG ,&nbsp;Shuyan WANG ,&nbsp;Hongsheng CHANG ,&nbsp;Lina LI","doi":"10.1016/j.cjac.2024.100397","DOIUrl":"10.1016/j.cjac.2024.100397","url":null,"abstract":"<div><p>Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive fibrotic disease with an unclear etiology and no effective treatment. This study aims to elucidate the pathogenic mechanism networks involving multiple targets and pathways in IPF. Extracts and metabolites of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), two well-known traditional Chinese medicines, have demonstrated therapeutic effects on IPF. However, the underlying mechanisms of AR and ASR remain unclear. Utilizing network pharmacology analysis, the disease targets associated with IPF were obtained from the GeneCards database and Online Mendelian Inheritance in Man (OMIM) database. Targets of AR and ASR were identified using the Traditional Chinese Medicine (TCM) Systems Pharmacology Database and Analysis Platform and Swiss Target Prediction. A proteinprotein interaction (PPI) network was subsequently constructed and analyzed using the STRING database and Cytoscape software. Gene ontology enrichment (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) analysis were conducted using DAVID. Additionally, a component-target-pathway network was employed to identify the main active components, and molecular docking was performed between these components and proteins encoded by key targets. Finally, in vitro studies were conducted based on network pharmacology. A total of 260 common targets between IPF and drug targets were identified and included in the PPI network, in which TNF, IL-6, STAT3, AKT1, VEGFA, SRC, EGFR, INS, JUN, and IL1B were predicted as key targets. These 260 targets were enriched in the PI3K-AKT signaling pathway, HIF-1 signaling pathway, TNF signaling pathway, MAPK signaling pathway, FOXO signaling pathway, and Pathways in cancer. Docking scores ranged from –4.1 to –9.5 kcal/mol, indicating a strong binding affinity between the main active compounds and key targets. In vitro studies have indeed shown that Quercetin and Magnolol can alleviate the expression of epithelial-mesenchymal transition in the A549 cells caused by IL-6. The treatment with AR and ASR resulted in a reduction of mRNA levels for key targets HIF-1α and α-SAM. Additionally, the protein expression levels of P-JAK2/ JAK2, P-STAT3/ STAT3, and α-SMA/ β-Actin were also reduced. These results support the therapeutic potential of AR and ASR in ameliorating pulmonary fibrosis and provide insight into the molecular mechanisms involved in their therapeutic effects.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 5","pages":"Article 100397"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000422/pdfft?md5=a17c40f539713e11b03685028ec25880&pid=1-s2.0-S1872204024000422-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141032014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}