IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-11 DOI: 10.1017/S0967199424000406

Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek

{"title":"Investigation of the effect of serotonin-activated semen washing medium on sperm motility at the molecular level: a pilot study.","authors":"Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek","doi":"10.1017/S0967199424000406","DOIUrl":"10.1017/S0967199424000406","url":null,"abstract":"In Assisted Reproductive Technologies (ART), efficient sperm preparation is vital for successful fertilization, with washing media enhancing the process. This pilot study examines the molecular-level impact of a new serotonin-containing sperm-washing medium (Prototype) on sperm motility and ROS metabolism, comparing it with commercially available media (Origio and Irvine). Semen samples from thirty-one individuals underwent preparation using the swim-up method post-semen analysis. Each sample was separately washed with the Prototype, Origio and Irvine mediums. ROS formation was determined through flow cytometric, and AT2R and PRDX2 protein levels, associated with sperm motility, were assessed via Western blot. Statistical evaluation compared the findings among the three outlined media. Significant differences were found among three washing media in terms of total and progressive motility. The Prototype medium showed the highest increase in both total (66%) and progressive motility (59%), while the control group exhibited the lowest increases (41% and 27.7%, respectively). Regarding ROS levels, the prototype (11.5%) and Origio (10.7%) groups demonstrated a notable decrease, contrasting with Irvine (25.8%). Molecular assessment revealed a significant elevation in AT2R protein levels in the prototype medium (59%), compared to other media. Additionally, an increase in PRDX2 protein levels was observed in the prototype medium, although this didn't reach statistical significance. Serotonin-activated washing media for sperm preparation can be a suitable choice for selecting high-quality sperm in ART. A broader molecular analysis with a larger sample size is required to explore the mechanisms and effectiveness of using a serotonin-containing sperm-washing medium in routine ART.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"396-404"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of L-carnitine in the control of oxidative stress and lipid β-oxidation during in vitro follicle growth, oocyte maturation, embryonic development and cryopreservation: a review. 左旋肉碱在体外卵泡生长、卵母细胞成熟、胚胎发育和冷冻保存过程中控制氧化应激和脂质β氧化的作用：综述。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-07 DOI: 10.1017/S096719942400039X

Allana Maria Freire Leitão, Bianca Regia Silva, Efigênia C Barbalho, Lais R M Paulino, Francisco das Chagas Costa, Fabricio Sousa Martins, Jose Roberto V Silva

引用次数: 0

Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus). 通过经阴道取卵术从 FSH 刺激的塔帕卡尔供体母牛（Bos indicus）中获取卵母细胞，对其发育能力进行形态分子评估。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-29 DOI: 10.1017/S0967199424000376

Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan

{"title":"Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus).","authors":"Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan","doi":"10.1017/S0967199424000376","DOIUrl":"10.1017/S0967199424000376","url":null,"abstract":"The study was conducted on indigenous Tharparkar cow (Bos indicus) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB+VE as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in Bos indicus cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB+VE oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"376-385"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the proliferative activity of germline progenitor cells in the adult ovary of the bat Artibeus jamaicensis. 鉴定蝙蝠Artibeus jamaicensis成体卵巢中生殖祖细胞的增殖活性。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI: 10.1017/S0967199424000364

Tania J Porras-Gómez, Norma Moreno-Mendoza

{"title":"Identification of the proliferative activity of germline progenitor cells in the adult ovary of the bat Artibeus jamaicensis.","authors":"Tania J Porras-Gómez, Norma Moreno-Mendoza","doi":"10.1017/S0967199424000364","DOIUrl":"10.1017/S0967199424000364","url":null,"abstract":"Until a few years ago, it was assumed that oocyte renewal did not take place in the ovary of adult organisms; however, the existence of germline progenitor cells (GPCs), which renew the ovarian follicular reserve, has now been documented in mammals. Specifically, in the adult ovary of bats, the presence of cells located in the cortical region with characteristics similar to GPCs, called adult cortical germ cells (ACGC), has been observed. One of the requirements that a GPC must fulfil is to be able to proliferate mitotically, so the evaluation of cell proliferation in ACGC is of utmost importance in order to be able to relate them to a parental lineage. Currently, there are several methods to determine cell proliferation, including BrdU labelling or the use of endogenous proliferation markers. Thus, the aim of this work was to evaluate the proliferative activity of ACGC in the adult ovary of the bat Artibeus jamaicensis, using different proliferation markers and correlating these with the protein expression of the transcription factor Oct4 and the germ line marker Ddx4. We found that the expression pattern of the proliferation markers BrdU, PCNA, Ki-67 and pH3 occurs at different times of the cell cycle, so co-localization of two or more of these markers allows us to identify proliferating cells. This allowed us to identify ACGC with proliferative capacity in the adult ovary of A. jamaicensis, suggesting that GPCs renew the follicle reserve during the adult life of the organism.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"366-375"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes. 补充硼酸可通过相关的多能基因和抗氧化基因促进体外生产的小鼠胚胎的发育。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-21 DOI: 10.1017/S0967199424000261

Ali Cihan Taskin, Ahmet Kocabay, Seref Gul, Gizem Nur Sahin, Sercin Karahuseyinoglu, I Halil Kavakli, Ibrahim Sogut

{"title":"Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes.","authors":"Ali Cihan Taskin, Ahmet Kocabay, Seref Gul, Gizem Nur Sahin, Sercin Karahuseyinoglu, I Halil Kavakli, Ibrahim Sogut","doi":"10.1017/S0967199424000261","DOIUrl":"10.1017/S0967199424000261","url":null,"abstract":"Boric acid (BA) is an important mineral for plants, animals and humans that assists metabolic function and has both positive and negative effects on biological systems. The present study aimed to investigate the effects of different concentrations of BA added to the culture media, the quality and in vitro development potential of mouse embryos. Superovulated C57Bl6/6j female mice were sacrificed ∼18 hours after human chorionic gonadotropin (hCG) injection. Single-cell-stage embryos were collected from the oviduct, divided into experiment groups and cultured in embryo medium with supplemented BA+ in 5% CO2 at 37 °C until 96 hours at the blastocyst stage. The blastocyst development rates of 0, 1.62 × 10-1, 1.62 × 10-2, 1.62 × 10-3 and 1.62 × 10-4 µM BA were 51.52%, 73.47%, 77.36% and 81.13%, respectively. The in vitro development rates were significantly higher in the 1.62 × 10-3 (p < 0.05) and 1.62 × 10-4 µM BA groups than in the control group (p < 0.001). These results indicated that low BA doses influenced embryo development by positively affecting in vitro development rates, embryo cell numbers, biochemical parameters and development at the molecular level by pluripotent and antioxidant genes. Therefore, BA seems to play an important role on in vitro embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"348-353"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and transcriptomic evaluations of infertile or subfertile Arunachali yak sperm. 不育或亚不育阿鲁纳恰里牦牛精子的基因组和转录组评估。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-17 DOI: 10.1017/S0967199424000194

Pranab Jyoti Das, Aneet Kour, Jyotika Bhati, Dwijesh Chandra Mishra, Mihir Sarkar

{"title":"Genomic and transcriptomic evaluations of infertile or subfertile Arunachali yak sperm.","authors":"Pranab Jyoti Das, Aneet Kour, Jyotika Bhati, Dwijesh Chandra Mishra, Mihir Sarkar","doi":"10.1017/S0967199424000194","DOIUrl":"10.1017/S0967199424000194","url":null,"abstract":"Sperm infertility or subfertility is detrimental to the precious highland germplasm like yak whose population has been gradually declining in India. Understanding the 'omic' landscape of infertile or subfertile yak sperm can reveal some interesting insights. In an attempt to do the same, this study considered the semen of infertile or subfertile yak bulls for whole-genome and transcriptome evaluations. DNA sequencing revealed that the yak sperm genome contains the necessary genes to carry out all the important biological processes related to the growth, development, survival and multiplication of an organism. Interestingly, RNA Seq results highlighted that genes like VAMP7, MYLK, ARAP2 and MARCH6 showed increased expression, while biological processes related to immune response (GO:0043308, GO:0002447, GO:0002278, GO:0043307, GO:0043312, GO:0002283, GO:0043299 and GO:0002446) were significantly overrepresented. These findings hint at a possible role played by immune system in regulating infertility or subfertility in yaks. Further, in-depth studies can validate these findings and help in improving our biological understanding in this area.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"341-347"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of rapamycin treatment on mouse ovarian follicle development in dehydroepiandrosterone-induced polycystic ovary syndrome mouse model. 雷帕霉素治疗对脱氢表雄酮诱导的多囊卵巢综合征小鼠模型卵泡发育的影响

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-05 DOI: 10.1017/S0967199424000388

Ecem Yildirim, Tugce Onel, Sami Agus, Elif Gunalan, Bayram Yilmaz, Mehmet Serif Aydin, Aylin Yaba

{"title":"The effect of rapamycin treatment on mouse ovarian follicle development in dehydroepiandrosterone-induced polycystic ovary syndrome mouse model.","authors":"Ecem Yildirim, Tugce Onel, Sami Agus, Elif Gunalan, Bayram Yilmaz, Mehmet Serif Aydin, Aylin Yaba","doi":"10.1017/S0967199424000388","DOIUrl":"10.1017/S0967199424000388","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a complex reproductive and endocrine disorder affecting 5-10% of women of reproductive age, but the pathophysiology of PCOS still remains unknown. Here, the aim of our study was to analyze the effects of rapamycin treatment that may regulate impaired hormonal levels and folliculogenesis in dehydroepiandrosterone (DHEA)-treated PCOS mouse. We hypothesized that rapamycin may ameliorate the negative effects of PCOS in DHEA-induced PCOS mouse model. The target of rapamycin (TOR) gene product is a serine/threonine kinase that has been implicated in the control of cell growth, proliferation and autophagy, and rapamycin is a potent inhibitor of mTORC1 pathway. In this study, for the first time, mTORC1 and activation products are presented at protein and mRNA levels after rapamycin treatment in DHEA-induced PCOS mouse ovary. We showed that rapamycin treatment may regulate follicular development, hormonal levels and provide ovulation in DHEA-induced PCOS mouse. Additionally, we assessed decreased primordial follicle reserve, increased number of primary and secondary follicles, corpus luteum structure forms again after 10 days of rapamycin treatment. This study presented here suggests rapamycin treatment regulates hormonal phenotype and folliculogenesis in the ovary and also mTOR signalling pathway in granulosa cells of DHEA-induced PCOS mouse ovary which may have potential to attenuate understanding the mechanism of dominant follicle selection and anovulatory infertility.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"386-395"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different temperatures on the embryonic development of the Lebranche mullet Mugil liza 不同温度对鲻鱼胚胎发育的影响

IF 1.7 4区生物学

Zygote Pub Date : 2024-09-19 DOI: 10.1017/s0967199424000285

João Vitor de Azevedo Manhães, Douglas da Cruz Mattos, Rômulo Alves Strassburguer, Ulysses da Silva Palma, Fabio Carneiro Sterzelecki, Marco Shizuo Owatari, Caio Magnotti, Vinicius Ronzani Cerqueira

{"title":"Effects of different temperatures on the embryonic development of the Lebranche mullet Mugil liza","authors":"João Vitor de Azevedo Manhães, Douglas da Cruz Mattos, Rômulo Alves Strassburguer, Ulysses da Silva Palma, Fabio Carneiro Sterzelecki, Marco Shizuo Owatari, Caio Magnotti, Vinicius Ronzani Cerqueira","doi":"10.1017/s0967199424000285","DOIUrl":"https://doi.org/10.1017/s0967199424000285","url":null,"abstract":"We herein investigated the influence of temperature on the embryonic development (from fertilisation to hatching) of Mugil liza larvae. For this purpose, oocytes (>600 μm) and sperm were obtained from breeding stock at the laboratory of marine fish culture (LAPMAR). After fertilisation, 1200 eggs were distributed in 12 cylindrical experimental units of 400 mL under four different temperatures 18, 22, 26 and 30 ºC, all in triplicate. Every 15 min until hatching, about 10 eggs were randomly sampled in each treatment. The eggs were visualized and photographed, and the classification of embryonic stages was performed. Temperature influenced the main events of the embryonic development of M. liza. More accelerated development was observed according to the increase in temperature until the gastrula phase. At temperatures of 22 and 26 °C, embryonic development occurred from fertilisation to hatching of the larvae. In the 18 °C treatment, it was verified that most of the embryos ceased development during the final phase of cleavage and the beginning of blastula formation, while in the 30 °C treatment patterns of embryo malformation were also verified, with erratic divisions of the blastomeres, resulting in irregular cells. Unlike what was observed at a temperature of 18 °C, none of the embryos incubated at 30 °C reached the blastopore closure phase, stopping in the gastrula. The larvae hatched in the treatments at 22 and 26 °C were viable and exhibited intense swimming, with a large amount of reserve material (yolk) and an evident drop of oil.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"27 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol attenuates doxorubicin-induced toxicity during in vitro culture of mouse-isolated preantral follicles 白藜芦醇可减轻小鼠离体前卵泡体外培养过程中多柔比星诱发的毒性

IF 1.7 4区生物学

Zygote Pub Date : 2024-09-19 DOI: 10.1017/s0967199424000315

G.A.L. Silva, A.P.O Monte, A.T.V. França, I.M. Mota, J.L. Oliveira Junior, K.O. Andrade, L.M. Souza, R.L.S. Silva, V.S. Guimarães, R.S. Barberino, R.C. Palheta Junior, J.E.J. Smitz, M.H.T. Matos

{"title":"Resveratrol attenuates doxorubicin-induced toxicity during in vitro culture of mouse-isolated preantral follicles","authors":"G.A.L. Silva, A.P.O Monte, A.T.V. França, I.M. Mota, J.L. Oliveira Junior, K.O. Andrade, L.M. Souza, R.L.S. Silva, V.S. Guimarães, R.S. Barberino, R.C. Palheta Junior, J.E.J. Smitz, M.H.T. Matos","doi":"10.1017/s0967199424000315","DOIUrl":"https://doi.org/10.1017/s0967199424000315","url":null,"abstract":"The aims of this study were to evaluate the doxorubicin concentration that induces toxic effects on in vitro culture of isolated mouse secondary follicles and to investigate whether resveratrol can inhibit or reduce this toxicity. Secondary follicles were isolated and cultured for 12 days in control medium (α-MEM+) or in α-MEM+ supplemented with doxorubicin (0.1 µg/ml) or different concentrations of resveratrol (0.5, 2, or 5 µM) associated with doxorubicin (0.1 µg/ml) (experiment 1). For experiment 2, follicles were cultured in α-MEM+ alone or supplemented with doxorubicin (0.3 µg/ml) or different concentrations of resveratrol (5 or 10 µM) associated or not with doxorubicin (0.3 µg/ml) (experiment 2). The endpoints analyzed were morphology (survival), antrum formation, follicular diameter, mitochondrial activity, glutathione (GSH) levels and DNA fragmentation. In the first experiment, doxorubicin (0.1 µg/ml) maintained survival and antrum formation similar to the control, while 5 µM resveratrol showed increased parameters, maintained mitochondrial activity and increased GSH levels compared to the control. In the second experiment, doxorubicin (0.3 µg/ml) reduced survival, antrum formation and follicular diameter compared to the control. Resveratrol at a concentration of 10 µM attenuated the damage caused by doxorubicin by improving follicular survival and did not present DNA fragmentation. In conclusion, supplementation of the in vitro culture medium with 0.3 µg/ml doxorubicin reduced the survival and impaired the development of mouse-isolated preantral follicles. Resveratrol at 10 µM reduced doxorubicin-induced follicular atresia, without DNA fragmentation in the follicles.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"20 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of calcium-sensing receptor (CaSR) expression and function in normal and abnormal human sperm and spermatogenic cells 正常和异常人类精子及生精细胞中钙感应受体（CaSR）表达和功能的比较分析

IF 1.7 4区生物学

Zygote Pub Date : 2024-09-18 DOI: 10.1017/s0967199424000091

Zhengli Qian, Keyan Luo, Mingzhe Zhang, Guanping Yao

{"title":"Comparative analysis of calcium-sensing receptor (CaSR) expression and function in normal and abnormal human sperm and spermatogenic cells","authors":"Zhengli Qian, Keyan Luo, Mingzhe Zhang, Guanping Yao","doi":"10.1017/s0967199424000091","DOIUrl":"https://doi.org/10.1017/s0967199424000091","url":null,"abstract":"Summary The calcium-sensing receptor (CaSR) is a critical mediator of calcium homeostasis in various tissues. Its role in human reproduction, especially in sperm function and male fertility, remains not fully elucidated. This study investigates the expression patterns of CaSR in normal and abnormal sperm and spermatogenic cells and evaluates its potential effect on sperm motility and morphology. Using immunohistochemistry (IHC), quantitative PCR (qPCR), we assessed the expression levels of CaSR in normal sperm, spermatogonia, and cases of asthenozoospermia, oligozoospermia, and teratozoospermia. In vitro functional assays were performed to analyze the effects of CaSR modulation on sperm motility under varying conditions, including the presence of specific CaSR agonists and antagonists. Our study revealed distinct patterns of CaSR expression in normal sperm and spermatogonia compared with those in abnormal sperm samples, particularly in cases of asthenozoospermia, oligozoospermia, and teratozoospermia. A marked decrease in CaSR expression was evident in these abnormal samples, highlighting its significance in normal sperm functionality. Functional assays further elucidated the role of CaSR in sperm motility. Activation of CaSR through specific agonists enhanced sperm motility, while inhibition by antagonists led to reduced motility. Our findings suggest that CaSR plays a significant role in maintaining sperm functionality and that changes in its expression may be associated with male infertility. These insights into the molecular underpinnings of sperm physiology highlight CaSR as a potential therapeutic target for treating certain forms of male infertility.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"49 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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