Safety evaluation of single-sperm cryopreservation technique applied in intracytoplasmic sperm injection

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-17 DOI:10.1017/s0967199424000078

Duanjun Zhang, Wenliang Yao, Mingliang Zhang, Lijuan Yang, Lin Li, Shujuan Liu, Xianglong Jiang, Yingli Sun, Shuonan Hu, Yufang Huang, Jie Xue, Xiaoting Zheng, Qi Xiong, Shenghui Chen, Haiqin Zhu

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引用次数: 0

Abstract

Intracytoplasmic sperm injection (ICSI) is a technique that directly injects a single sperm into the cytoplasm of mature oocytes. Here, we explored the safety of single-sperm cryopreservation applied in ICSI. This retrospective study enrolled 186 couples undergoing ICSI-assisted pregnancy. Subjects were allocated to the fresh sperm (group A)/single-sperm cryopreservation (group B) groups based on sperm type, with their clinical baseline/pathological data documented. We used ICSI-compliant sperm for subsequent in vitro fertilization and followed up on all subjects. The recovery rate/cryosurvival rate/sperm motility of both groups, the pregnancy/outcome of women receiving embryo transfer, and the delivery mode/neonatal-related information of women with successful deliveries were recorded. The clinical pregnancy rate, cumulative clinical pregnancy rate, abortion rate, ectopic pregnancy rate, premature delivery rate, live birth delivery rate, neonatal birth defect rate, and average birth weight were analyzed. The two groups showed no significant differences in age, body mass index, ovulation induction regimen, sex hormone [anti-Müllerian hormone (AMH)/follicle-stimulating hormone (FSH)/luteinizing hormone (LH)] levels, or oocyte retrieval cycles. The sperm recovery rate (51.72%-100.00%) and resuscitation rate (62.09% ± 16.67%) in group B were higher; the sperm motility in the two groups demonstrated no significant difference and met the ICSI requirements. Group B exhibited an increased fertilization rate, decreased abortion rate, and increased safety versus group A. Compared with fresh sperm, the application of single-sperm cryopreservation in ICSI sensibly improved the fertilization rate and reduced the abortion rate, showing higher safety.

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卵胞浆内单精子冷冻保存技术的安全性评估

卵胞浆内单精子显微注射（ICSI）是一种将单个精子直接注射到成熟卵母细胞胞浆内的技术。在此，我们探讨了应用于 ICSI 的单精子冷冻保存的安全性。这项回顾性研究共纳入了186对接受ICSI辅助妊娠的夫妇。受试者根据精子类型被分配到新鲜精子组（A 组）和单精子冷冻保存组（B 组），并记录了他们的临床基础/病理数据。我们使用符合 ICSI 标准的精子进行体外受精，并对所有受试者进行随访。我们记录了两组受试者的恢复率/冷冻存活率/精子活力、接受胚胎移植妇女的妊娠情况/结果，以及成功分娩妇女的分娩方式/新生儿相关信息。分析了临床妊娠率、累积临床妊娠率、流产率、异位妊娠率、早产率、活产率、新生儿出生缺陷率和平均出生体重。两组患者在年龄、体重指数、促排卵方案、性激素[抗缪勒氏管激素（AMH）/卵泡刺激素（FSH）/黄体生成素（LH）]水平或卵母细胞获取周期方面无明显差异。B 组的精子回收率（51.72%-100.00%）和复苏率（62.09% ± 16.67%）较高；两组的精子活力无显著差异，均符合 ICSI 要求。与新鲜精子相比，单精子冷冻技术在ICSI中的应用合理地提高了受精率，降低了流产率，安全性更高。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.