IF 1.5 4区生物学

Zygote Pub Date : 2025-07-08 DOI: 10.1017/S0967199425100063

Zahra Bashiri, Mansoureh Movahedin, Vahid Pirhajati, Hamidreza Asgari, Morteza Koruji

引用次数: 0

Non-invasive human preimplantation embryos sex determination using STR-based fluorescent multiplex PCR on days 3 and 5 post-fertilization. 基于str的荧光多重PCR在受精后第3天和第5天的无创人类着床前胚胎性别测定。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-07-10 DOI: 10.1017/S0967199422000351

Maryam Zare, Mehdi Totonchi, Hamid Gourabi, Mohammadreza Zamanian, Reza Mohammadi, Sirous Zeinali, Maryam Mohammadi, Poopak Eftekhari-Yazdi

{"title":"Non-invasive human preimplantation embryos sex determination using STR-based fluorescent multiplex PCR on days 3 and 5 post-fertilization.","authors":"Maryam Zare, Mehdi Totonchi, Hamid Gourabi, Mohammadreza Zamanian, Reza Mohammadi, Sirous Zeinali, Maryam Mohammadi, Poopak Eftekhari-Yazdi","doi":"10.1017/S0967199422000351","DOIUrl":"10.1017/S0967199422000351","url":null,"abstract":"This study aimed to investigate the efficacy of cell-free DNA (CF-DNA) in the spent cleavage and blastocyst medium versus blastomere biopsy for sex identification using short tandem repeat (STR) markers for the first time. In total, 39 samples of spent culture medium (SCM) from six couples were collected of which 28 samples were CF-DNA from blastocoel fluid + SCM (day 5) and 11 samples from SCM alone (day 3). The frequencies of allele dropout (ADO), fail rate and informativity markers were considered. The relationship between the morphology of embryos and ADO and the fail number of all markers was investigated. Sex identification rate between CF-DNA isolated from culture medium and fluorescence in situ hybridization (FISH) was then compared with measurement of Agreement Kappa (AK). The highest frequency of informative markers belonged to DXS6801 and HPRT. There was no relationship between the ADO number of all markers and embryo morphology. A significant difference was seen between embryo morphology and fail numbers. AK value between CF-DNA isolated from culture medium and FISH was 0.516, which is moderate. The ability of CF-DNA to detect the correct diagnosis of males and females showed that all values of specificity, sensitivity, positive predictive value, and negative predictive value were 100%. The presence of embryonic CF-DNA in the SCM on day 3 as well as blastocyst medium on day 5 using STR-based multiplex PCR is approximately consistent with FISH for sex identification. Advances in DNA extraction, amplification technique, and testing may allow for preimplantation genetic testing for aneuploidy (PGT-A) and monogenic/single-gene disorders (PGT-M) as a non-invasive approach without biopsy in the future either in sex determination or chromosomal abnormality.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"145-155"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovarian structure and oogenesis in King Tiger Pleco, L333, Hypancistrus seideli, (Siluriformes: Loricariidae) from the Amazon region. 亚马逊地区王虎Pleco， L333, Hypancistrus seideli, （siluriforma: Loricariidae）卵巢结构及卵发生。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-08-19 DOI: 10.1017/S0967199425100087

Ivana Kerly da Silva Viana, Iann Leonardo Pinheiro Monteiro, Ryuller Gama Abreu, Maria Auxiliadora Pantoja Ferreira, Leandro Melo de Sousa, Rossineide Martins da Rocha

{"title":"Ovarian structure and oogenesis in King Tiger Pleco, L333, Hypancistrus seideli, (Siluriformes: Loricariidae) from the Amazon region.","authors":"Ivana Kerly da Silva Viana, Iann Leonardo Pinheiro Monteiro, Ryuller Gama Abreu, Maria Auxiliadora Pantoja Ferreira, Leandro Melo de Sousa, Rossineide Martins da Rocha","doi":"10.1017/S0967199425100087","DOIUrl":"10.1017/S0967199425100087","url":null,"abstract":"In this study, we describe the ovarian structure and oogenesis up to the final maturation of oocytes of Hypancistrus seideli. A total of sixty females were used for gonadal analysis and subsequently submitted to light and scanning electron microscopy analyses. Four maturation stages were defined: immature, maturing, mature, and spawned. The oocytes were classified into four stages (I–IV), and the presence of atretic oocytes and post-ovulatory follicles was demonstrated. During oocyte development, changes were observed in color, size, and shape, as well as in the formation of the follicular complex. These results may support reproductive management in captivity, since the species has great commercial importance in the international ornamental fish market and lacks established reproductive protocols in aquaculture. To our knowledge, this is the first morphological characterization of oogenesis in this species, providing original and detailed data that may contribute to the development of captive breeding protocols and to reducing pressure on natural stocks.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"179-185"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues. 乙酰左旋肉碱增加培养牛卵巢组织的卵泡存活率和基质细胞密度。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-06-03 DOI: 10.1017/S0967199425000103

Allana M F Leitão, Antonia V N Azevedo, Bianca R Silva, Efigênia C Barbalho, Ernando I Teixeira de Assis, Francisco C Costa, Fabricio S Martins, José R V Silva

{"title":"Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues.","authors":"Allana M F Leitão, Antonia V N Azevedo, Bianca R Silva, Efigênia C Barbalho, Ernando I Teixeira de Assis, Francisco C Costa, Fabricio S Martins, José R V Silva","doi":"10.1017/S0967199425000103","DOIUrl":"10.1017/S0967199425000103","url":null,"abstract":"This study aimed to evaluate the effects of acetyl-L-carnitine on follicle survival and growth, stromal cell density and extracellular matrix, as well as on the expression of mRNA for nuclear factor erythroid 2-related factor (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and peroxiredoxin 6 (PRDX6) in cultured bovine ovarian cortical tissues. Ovarian fragments (3 × 3 × 1 mm) were cultured for 6 days in α-MEM+ alone or supplemented with 10, 50 or 100 μM acetyl-L-carnitine at 38.5°C with 5% CO2 in humidified air. Before (non-cultured tissues) and after culture, the ovarian fragments were fixed in 4% paraformaldehyde for 12 h for histological analysis or stored at -80ºC for mRNA expression analysis of NRF2, SOD, CAT, PRDX6 and GPX1. The results showed that 100 μM acetyl-L-carnitine increased the percentages of morphologically normal follicles and stromal cell density in cultured ovarian tissues. On the other hand, acetyl-L-carnitine did not influence the percentage of collagen in ovarian tissue nor the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1. In conclusion, 100 μM acetyl-L-carnitine increased follicle survival and stromal cell density in cultured bovine ovarian tissues but does not influence collagen fibre distribution or the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"125-131"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol conjugated with silica nanoparticles used for in vitro bovine oocytes maturation. 白藜芦醇结合二氧化硅纳米颗粒用于牛卵母细胞体外成熟。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-07-07 DOI: 10.1017/S096719942510004X

Soraia Rage Rezende, Mayara Mafra Soares, Graciele Freitas Cardoso, Deize de Cássia Antonino Rissato, Giovanna Faria de Moraes, Gustavo Pereira Cadima, Kele Amaral Alves, Benner Geraldo Alves, Tatiane Moraes Arantes, Rodrigo Otavio Decaria de Salles Rossi, Ricarda Maria Dos Santos

{"title":"Resveratrol conjugated with silica nanoparticles used for in vitro bovine oocytes maturation.","authors":"Soraia Rage Rezende, Mayara Mafra Soares, Graciele Freitas Cardoso, Deize de Cássia Antonino Rissato, Giovanna Faria de Moraes, Gustavo Pereira Cadima, Kele Amaral Alves, Benner Geraldo Alves, Tatiane Moraes Arantes, Rodrigo Otavio Decaria de Salles Rossi, Ricarda Maria Dos Santos","doi":"10.1017/S096719942510004X","DOIUrl":"10.1017/S096719942510004X","url":null,"abstract":"The objective was to evaluate the use of resveratrol conjugated with silica nanoparticles during the in vitro maturation of bovine oocytes. The oocytes were divided into the following treatment groups during the maturation process: control (n = 159), resveratrol 0.5 μM (n = 158), resveratrol 1 μM (n = 155), nanoparticles conjugated with 0.5 μM resveratrol (n = 159), and nanoparticles conjugated with 1 μM resveratrol (n = 158). Several parameters were assessed, including cumulus oophorus size, reactive oxygen species (ROS) production, oocyte nuclear maturation, cell apoptosis, cleavage rates, and blastocyst production rates. Statistical analysis was conducted using Sigma Plot software (version 11) and SAS Studio, with statistical significance defined as P ≤ 0.05 for the main effects and interactions. The results indicated that the cumulus oophorus size was smaller in the resveratrol 1 μM treatment group, and the oocyte size was reduced in the nanoparticle 1 μM treatment group. No significant differences were detected between the treatment groups in terms of ROS production, oocyte maturation, or cell apoptosis. However, the resveratrol 1 μM treatment group exhibited decreased rates of cleavage and blastocyst formation. In contrast, the nanoparticles 0.5 μM and 1.0 μM treatments showed improved cleavage and blastocyst rates compared with the resveratrol 1.0 μM treatment group. In summary, while resveratrol alone at 1 μM concentration had a negative impact on cleavage and blastocyst rates, the use of silica nanoparticles conjugated with resveratrol (both 0.5 μM and 1 μM) enhanced these outcomes, suggesting a potential advantage in using nanoparticle-conjugated resveratrol for the in vitro maturation of bovine oocytes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"139-144"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage). 使用第 3 天（卵裂期）和第 4 天（蜕膜期）的胚胎，在新鲜和冷冻周期中进行化学妊娠。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-03-21 DOI: 10.1017/S0967199425000048

Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast

{"title":"Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage).","authors":"Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast","doi":"10.1017/S0967199425000048","DOIUrl":"10.1017/S0967199425000048","url":null,"abstract":"With the advancement in the embryo culture media, which supports nutrient requirements of embryos up to 5 to 6 days, there's a chance to select more viable embryos, which are more likely to result in pregnancy, compared to earlier stages. Also, there is a controversy regarding the frozen embryo transfer compared to the fresh type. To compare the chemical pregnancy rates between fresh embryo transfer (ET), and frozen embryo transfer (FET), on day 3 (cleavage), and day 4 (morula) of development. In this retrospective study, data of 242 fresh and 758 frozen embryo transfer cycles were obtained in one infertility center in Isfahan, Iran. The study's groups were assigned based on the day of fresh or frozen embryo transfer (day 3, or day 4 embryos) and the embryo grading. Chemical pregnancy was the main outcome measurement (implantation rates). The chemical pregnancy rate was higher in the good quality frozen embryo day 3 and transfer on day 4 group (40.1%). This rate was near the results of transferring the good quality frozen embryo on day 4 (39.2 %). There was no significant difference in the chemical pregnancy rate related to the number of transferred embryos (p = 0.55). The higher PRs, when the embryos were transferred on day 4, provided further support for the morula stage embryo transfer, possibly because of better synchrony with the endometrium. It is concluded that morula/compact embryos are good candidates for embryo transfer, which simultaneously reduces the number of transferred embryos.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"117-124"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of time-lapse culture dishes with refrigerated medium, rather than pre-warmed, may result in better clinical outcomes. 用冷藏培养基而不是预热培养基制备延时培养皿可能会产生更好的临床结果。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-07-07 DOI: 10.1017/S0967199425100051

Yasong Geng, Fangfang Dai, Zhiwei Yang, Guozhen Li, Linlin Tao, Haoyang Dai, Shusong Wang, Bo Zheng

{"title":"Preparation of time-lapse culture dishes with refrigerated medium, rather than pre-warmed, may result in better clinical outcomes.","authors":"Yasong Geng, Fangfang Dai, Zhiwei Yang, Guozhen Li, Linlin Tao, Haoyang Dai, Shusong Wang, Bo Zheng","doi":"10.1017/S0967199425100051","DOIUrl":"10.1017/S0967199425100051","url":null,"abstract":"This study aimed to compare the clinical outcomes of using refrigerated versus pre-warmed media for preparing time-lapse dishes in in vitro fertilization (IVF). Patients undergoing their first IVF/ICSI cycle were divided into two groups. The control group used pre-warmed culture media, while the experimental group used refrigerated culture media. The osmotic pressure of the culture droplets in both groups was tested. No statistical differences were found between the two groups' basic data. The proportion of air microbubbles affecting imaging significantly decreased (4.55% vs. 37.97%, P < 0.001) when using pre-warmed media. However, the blastocyst formation rate (56.62% vs. 49.70%, P = 0.046) and total high-quality embryo rate (22.26% vs. 17.06%, P = 0.047) were significantly higher in the refrigerated media group compared to the pre-warmed media group. The higher rate of high-quality embryos in the refrigerated media group might result in a higher single embryo transfer rate (45.10% vs. 18.52%, P = 0.020) and implantation rate (58.23% vs. 34.69%, P = 0.010). From day -1 to day 1, osmolality increased, with the P-3.5 group showing a significant elevation compared to the other three groups. After 5 days of incubation, the osmotic pressure of group R-4.0 was significantly lower than that of groups P-3.5, P-4.0 and P-3.5. In conclusion, refrigerated culture media dishes helped stabilize the osmotic pressure of the culture microenvironment and reduce water evaporation. The refrigerated group showed a higher rate of high-quality embryos and live births, although pre-warmed culture media effectively reduced the occurrence of air microbubbles that affect embryo imaging in the next day's dishes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"132-138"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do serum AMH levels affect the incidence rate of multinucleated embryos in ICSI cycles? 血清AMH水平是否影响ICSI周期中多核胚胎的发生率？

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-08-13 DOI: 10.1017/S0967199425100105

Sümeyye Mermi, Nafiye Yılmaz, Banu Seven, Mehmet Caner Özer, Özlem Moraloglu Tekin

{"title":"Do serum AMH levels affect the incidence rate of multinucleated embryos in ICSI cycles?","authors":"Sümeyye Mermi, Nafiye Yılmaz, Banu Seven, Mehmet Caner Özer, Özlem Moraloglu Tekin","doi":"10.1017/S0967199425100105","DOIUrl":"10.1017/S0967199425100105","url":null,"abstract":"A blastomere containing more than one nucleus is defined as a multinucleated blastomere. In our study, we aimed to investigate the relationship between serum anti-Mullerian hormone (AMH) levels and multinucleated (MLN) embryos, one of the parameters indicating embryo quality, in intracytoplasmic sperm injection (ICSI) cycles. The results of 888 ICSI cycles of patients aged 19-45 years attending an ART (assisted reproductive technology) clinic were retrospectively analysed. Cycles with at least one MLN embryo were defined as the study group (n = 237) and cycles without MLN embryos as the control group (n = 651). Univariate and multivariate logistic regression analyses were used to determine the risk factors affecting the dependent qualitative variables. The effect of AMH levels on multinucleation was found to be a significant risk factor (p < 0.001). One unit increase in AMH levels increases the risk of the presence of MLN embryos by 1.12 times. The mean MLN embryos/total embryo ratio in the group with clinical pregnancy was 0.34 ± 0.18, while the mean MLN embryos/total embryo ratio in the group that did not achieve clinical pregnancy was 0.47 ± 0.3 (p = 0.010). The presence of an MLN embryo has been associated with poor embryo development and ART outcomes. Parameters that can predict the formation of MLN embryos before treatment are crucial for the determination of the pregnancy rate. According to our results, serum AMH levels can be used as a predictive marker for the formation of MLN embryos.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"156-162"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144838028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of oocyte and sperm parameters on the morphological quality and the aneuploidy status of the ICSI embryos: a mathematical modelling approach. 卵母细胞和精子参数对ICSI胚胎形态质量和非整倍体状态的影响：一种数学建模方法。

IF 1.4 4区生物学

Zygote Pub Date : 2025-06-01 Epub Date: 2025-08-19 DOI: 10.1017/S0967199425100099

Hakan Aytacoglu, David Amilo, Bilgen Kaymakamzade, Evren Hincal, Onder Coban, Pinar Tulay

{"title":"Influence of oocyte and sperm parameters on the morphological quality and the aneuploidy status of the ICSI embryos: a mathematical modelling approach.","authors":"Hakan Aytacoglu, David Amilo, Bilgen Kaymakamzade, Evren Hincal, Onder Coban, Pinar Tulay","doi":"10.1017/S0967199425100099","DOIUrl":"10.1017/S0967199425100099","url":null,"abstract":"Recently, mathematical and computational approaches have been incorporated into ICSI interventions as guiding tools. However, those tools carry no prognostic potential. Improving this capability may enhance ICSI attempts and assist clinicians working in infertility clinics. This study, thus aimed to investigate whether parental parameters could have predictive potential for the quality of resulting embryos with the ICSI approach using mathematical modelling techniques. Patient data including follicle number, MI and MII oocyte numbers, sperm number, sperm morphology and motility for 765 distinct couples attending British Cyprus IVF hospital was collected. Furthermore, morphological quality data as well as aneuploidy status for the 4123 resultant embryos were obtained. Regression analyses were conducted to observe the possible correlations between parental parameters and embryo quality and ploidy. Correlation analyses showed that follicle and oocyte numbers, as well as sperm parameters can be indicative of morphological quality of resulting embryos via ICSI (p values < 0.05). On the other hand, aneuploidy prediction remains too complicated to be predicted solely by these parameters (p values > 0.05). This study indicates a predictive potential of infertility measurements for male and female partners on ICSI success and is expected to act as a basis for the development of prognostic softwares to be used in IVF clinics.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"163-178"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies. 了解CD-117基因在小鼠睾丸生殖细胞中的表达：体外和体内研究。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-17 DOI: 10.1017/S0967199424000455

Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi

{"title":"Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies.","authors":"Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi","doi":"10.1017/S0967199424000455","DOIUrl":"10.1017/S0967199424000455","url":null,"abstract":"Background: The proto-oncogene tyrosine kinase receptor encoded by the W locus (CD-117) has been confirmed to be critical to the processes of germ cell proliferation, migration and survival in the rodent. The purpose of the present study was to examine the expression of germ cell-specific CD-117 marker in testis and germ line stem cells (GSCs). The aim of this study was analysis of CD-117 expression as germ cell marker in the seminiferous tubule of mice.Materials and methods: In this experimental study, we employed a comprehensive array of techniques to scrutinize the expression of CD-117. Our analysis encompassed the utilization of immunocytochemistry, immunohistochemistry, Fluidigm real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), and flow cytometry methodologies.Results: The Immuno-history-fluorescent analysis revealed the distribution of the germ cell marker CD-117 in the differentiated compartment of seminiferous tubules. High-magnification of confocal microscopy analysis showed surface expression of CD-117 in testis section. Whereas isolated GSCs colonies clearly express the germ-specific protein CD-117, TSCs (testicular stromal cells) were negative for this marker. Fluidigm real-time RT-PCR result demonstrated a significant expression (P < 0.001) of CD-117 in the neonate and adult GSCs compared to TSCs cells. Similarly, flow cytometry analysis confirmed expression of CD-117 in the GSCs colonies and testis cells.Conclusion: These results discriminate in spite of stage-specific ectopic, expression of CD-117 is a specific germ cell marker for proliferation and differentiation of GSCs into sperm, and can be beneficial for understanding of the signalling pathways related to differentiation of GSCs.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"80-84"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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