IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-11-24 DOI: 10.1017/S0967199423000540

Yan Zhu, Zhen Zhang, Guang-Li Zhang, Man-Xi Jiang

{"title":"Effects of multi-gradient equilibration during vitrification on oocyte survival and embryo development in mice.","authors":"Yan Zhu, Zhen Zhang, Guang-Li Zhang, Man-Xi Jiang","doi":"10.1017/S0967199423000540","DOIUrl":"10.1017/S0967199423000540","url":null,"abstract":"Vitrification has been widely used for oocyte cryopreservation, but there is still a need for optimization to improve clinical outcomes. In this study, we compared the routine droplet merge protocol with modified multi-gradient equilibration vitrification for cryopreservation of mouse oocytes at metaphase II. Subsequently, the oocytes were thawed and subjected to intracytoplasmic sperm injection (ICSI). Oocyte survival and spindle status were evaluated by morphology and immunofluorescence staining. Moreover, the fertilization rates and blastocyst development were examined in vitro. The results showed that multi-gradient equilibration vitrification outperformed droplet merge vitrification in terms of oocyte survival, spindle morphology, blastocyst formation, and embryo quality. In contrast, droplet merge vitrification exhibited decreasing survival rates, a reduced proportion of oocytes with normal spindle morphology, and lower blastocyst rates as the number of loaded oocytes increased. Notably, when more than six oocytes were loaded, reduced oocyte survival rates, abnormal oocyte spindle morphology, and poor embryo quality were observed. These findings highlight that the vitrification of mouse metaphase II oocytes by the modified multi-gradient equilibration vitrification has the advantage of maintaining oocyte survival, spindle morphology, and subsequent embryonic development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138300162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive outcomes with delayed blastocyst development: the clinical value of day 7 euploid blastocysts in frozen embryo transfer cycles. 延迟囊胚发育的生殖结局:第7天整倍体囊胚在冷冻胚胎移植周期中的临床价值。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-11-13 DOI: 10.1017/S0967199423000485

Andrea Abdala, Ibrahim Elkhatib, Aşina Bayram, Ahmed El-Damen, Laura Melado, Daniela Nogueira, Barbara Lawrenz, Human M Fatemi

{"title":"Reproductive outcomes with delayed blastocyst development: the clinical value of day 7 euploid blastocysts in frozen embryo transfer cycles.","authors":"Andrea Abdala, Ibrahim Elkhatib, Aşina Bayram, Ahmed El-Damen, Laura Melado, Daniela Nogueira, Barbara Lawrenz, Human M Fatemi","doi":"10.1017/S0967199423000485","DOIUrl":"10.1017/S0967199423000485","url":null,"abstract":"Embryos of optimal development reach blastocyst stage 116 ± 2 h after insemination. Usable D7 blastocysts represent nearly 5% of embryos in IVF with acceptable pregnancy and live birth rates, however data are still limited. Therefore, this study aimed to analyze the ongoing pregnancy rate (OPR) of D7 blastocysts in single euploid frozen embryo transfer (FET) cycles. An observational study was performed including 1527 FET cycles with blastocysts biopsied on D5 (N = 855), D6 (N = 636) and D7 (N = 36). Blastocysts were classified as good (AA/AB/BA), fair (BB) or poor (AC/BC/CC/CA/CB) (Gardner scoring). FETs were performed in natural cycles (NC) or hormone replacement therapy (HRT) cycles. Patient's age differed significantly between D5, D6 and D7 blastocysts FET cycles (33.2 ± 5.6, 34.4 ± 5.3 and 35.9 ± 5.2, P < 0.001). OPRs were higher when D5 euploid blastocysts were transferred compared with D6 and D7 (56.0% vs. 45.3% and 11.1%, P < 0.001). Poor quality blastocysts were predominant in D7 blastocyst FET cycles (good quality: 35.4%, 27.2%, 5.6%; fair quality: 52.1%, 38.5%, 11.1%; poor quality: 12.5%, 34.3%, 83.3%, P < 0.001 for D5, D6 and D7 blastocysts; respectively). OPR was significantly reduced by D7 blastocyst FETs (OR = 0.23 [0.08;0.62], P = 0.004), patient's BMI (OR = 0.96 [0.94;0.98], P < 0.001), HRT cycles (OR = 0.70 [0.56;0.88], P = 0.002) and poor quality blastocysts (OR = 0.33 [0.24;0.45], P < 0.001). OPR is significantly reduced with D7 compared with D5/D6 euploid blastocysts in FET cycles. The older the patient, the more likely they are to have an FET cycle with blastocysts biopsied on D7, therefore culturing embryos until D7 can be a strategy to increase OPR outcomes in patients ≥38 years.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of klotho protein or klotho knockdown in porcine oocytes at different stages. klotho蛋白或klotho敲除对不同阶段猪卵母细胞的影响。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-10-31 DOI: 10.1017/S096719942300045X

Eun Pyo Kim, Do Yon Kim, Changhoon Park, Seung-Min Yoo, Myung-Shin Lee, Geon A Kim

{"title":"Effects of klotho protein or klotho knockdown in porcine oocytes at different stages.","authors":"Eun Pyo Kim, Do Yon Kim, Changhoon Park, Seung-Min Yoo, Myung-Shin Lee, Geon A Kim","doi":"10.1017/S096719942300045X","DOIUrl":"10.1017/S096719942300045X","url":null,"abstract":"Klotho is a protein that plays different functions in female fertility. We have previously reported that klotho protein supplementation during in vitro maturation improves porcine embryo development, while klotho knockout for somatic cell cloning completely blocks full-term pregnancy in vivo. However, the effects of the microinjection of klotho protein or klotho knockdown dual vector in porcine embryos at different time points and the specific molecular mechanisms remain largely unknown. In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted before in vitro maturation was significantly higher than that after klotho protein insertion into parthenogenetically activated embryos. These results indicated that klotho knockdown may impair embryo development into blastocyst irrespective of injection timing. In addition, klotho protein injection timing in pig embryos may be an important factor for regulating embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71414349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The optimal frozen embryo transfer strategy for the recurrent implantation failure patient without blastocyst freezing: thawing day 3 embryos and culturing to day 5 blastocysts. 反复着床失败患者无囊胚冷冻的最佳冷冻胚胎移植策略:解冻第3天胚胎，培养第5天囊胚。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-11-16 DOI: 10.1017/S0967199423000503

Xiang Li, Youman Zeng, Juan He, Bowen Luo, Xiongcai Lu, Lingling Zhu, Zengyu Yang, Fuman Cai, Sheng-Ao Chen, Yudi Luo

{"title":"The optimal frozen embryo transfer strategy for the recurrent implantation failure patient without blastocyst freezing: thawing day 3 embryos and culturing to day 5 blastocysts.","authors":"Xiang Li, Youman Zeng, Juan He, Bowen Luo, Xiongcai Lu, Lingling Zhu, Zengyu Yang, Fuman Cai, Sheng-Ao Chen, Yudi Luo","doi":"10.1017/S0967199423000503","DOIUrl":"10.1017/S0967199423000503","url":null,"abstract":"This study aimed to investigate the optimal frozen embryo transfer (FET) strategy for recurrent implantation failure (RIF) patients with three consecutive failed cleaved embryo implantations and no blastocyst preservation. This retrospective analysis was divided into three groups based on the FET strategy: thawed day 3 embryo transfer (D3 FET group); and extended culture of frozen-thawed day 3 embryos to day 5 blastocysts transfer (D3-D5 FET group); thawed blastocyst transfer (D5 FET group). Transplant cycle data were compared between the three groups. In total, 43.8% of vitrified-thawed cleavage embryos developed into blastocysts. Analysis of the three transplantation strategies showed that, compared with the D3 FET group, D3-D5 had a significantly better hCG-positivity rate and live-birth rate (P < 0.05). Pregnancy outcomes in the D3-D5 FET group and D5 FET group were similar regarding hCG-positivity rate, implantation rate, clinical pregnancy rate, and live-birth rate. Our findings propose two potentially valuable transfer strategies for patients experiencing repeated implantation failures. The D3-D5 FET approach presents a greater potential for selecting promising embryos in cases without blastocyst preservation; however, this strategy does entail the risk of cycle cancellation. Conversely, in instances where blastocyst preservation is an option, prioritizing consideration of the D5 FET strategy is recommended.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes. 体外成熟在合成输卵管液增加基因表达与质量和脂质代谢在牛卵母细胞。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-11-13 DOI: 10.1017/S0967199423000473

Adriano Felipe Mendes, Raquel Zaneti Puelker, Lilian Francisco Arantes de Souza, Andréa Renesto Coimbra Jacintho, Priscila Helena Dos Santos, Ines Cristina Giometti, Sheila Merlo Firetti, Anthony César de Souza Castilho, Marilice Zundt, Claudia Maria Bertan Membrive, Caliê Castilho

{"title":"In vitro maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes.","authors":"Adriano Felipe Mendes, Raquel Zaneti Puelker, Lilian Francisco Arantes de Souza, Andréa Renesto Coimbra Jacintho, Priscila Helena Dos Santos, Ines Cristina Giometti, Sheila Merlo Firetti, Anthony César de Souza Castilho, Marilice Zundt, Claudia Maria Bertan Membrive, Caliê Castilho","doi":"10.1017/S0967199423000473","DOIUrl":"10.1017/S0967199423000473","url":null,"abstract":"Traditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus-oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (SOF + CLA group). COCs matured with SOF showed a higher relative abundance of mRNA of quality indicators gremlin 1 (GREM1) and prostaglandin-endoperoxide synthase 2 (PTGS2) in oocytes, and GREM1 in cumulus cells compared with in the M199 group. SOF medium COCs had a higher relative abundance of fatty acid desaturase 2 (FADS2) compared with the M199 group, which is essential for lipid metabolism in oocytes. Furthermore, the abundance of stearoyl-coenzyme A desaturase 1 (SCD1) in oocytes matured with SOF was not influenced by the addition of CLA, whereas the relative abundance of SCD1 was reduced in M199 medium with CLA. We concluded that maturation in SOF medium results in a greater abundance of genes linked to quality and lipidic metabolism in oocytes, regardless of the addition of CLA.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of BAK, BAX and MAD2L1 gene expression in human aneuploid blastocysts. 人非整倍体囊胚中BAK、BAX和MAD2L1基因表达的研究。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-11-23 DOI: 10.1017/S0967199423000539

M Ahmed, H Aytacoglu, O Coban, P Tulay

{"title":"Investigation of BAK, BAX and MAD2L1 gene expression in human aneuploid blastocysts.","authors":"M Ahmed, H Aytacoglu, O Coban, P Tulay","doi":"10.1017/S0967199423000539","DOIUrl":"10.1017/S0967199423000539","url":null,"abstract":"Maintaining genomic stability is crucial for normal development. At earlier stages of preimplantation development, as the embryonic genome activation is not fully completed, the embryos may be more prone to abnormalities. Aneuploidies are one of the most common genetic causes of implantation failure or first-trimester miscarriages. Apoptosis is a crucial mechanism to eliminate damaged or abnormal cells from the organism to enable healthy growth. Therefore, this study aimed to determine the relationship between the expression levels of genes involved in apoptosis in human aneuploid and euploid blastocysts. In total, 32 human embryos obtained from 21 patients were used for this study. Trophectoderm biopsies were performed and next-generation screening was carried out for aneuploidy screening. Total RNA was extracted from each blastocyst separately and cDNA was synthesized. Gene expression levels were evaluated using RT-PCR. The statistical analysis was performed to evaluate the gene expression level variations in the euploid and aneuploid embryos, respectively. The expression level of the BAX gene was significantly different between the aneuploid and euploid samples. BAX expression levels were found to be 1.5-fold lower in aneuploid cells. However, the expression levels of BAK and MAD2L1 genes were similar in each group. This study aimed to investigate the possible role of genes involved in apoptosis and aneuploidy mechanisms. The findings of this investigation revealed that the BAX gene was expressed significantly differently between aneuploid and euploid embryos. Therefore, it is possible that the genes involved in the apoptotic pathway have a role in the aneuploidy mechanism.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138296161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluative effectiveness of follicular output rate, ovarian sensitivity index, and ovarian response prediction index for the ovarian reserve and response of low-prognosis patients according to the POSEIDON criteria: a retrospective study. 根据POSEIDON标准，卵泡产出率、卵巢敏感性指数和卵巢反应预测指数对低预后患者卵巢储备和反应的评估有效性：一项回顾性研究。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-09-22 DOI: 10.1017/S0967199423000382

Zhilan Chen, Wei Li, Shufang Ma, Yanmin Li, Liqun Lv, Kecheng Huang, Aidong Gong

{"title":"Evaluative effectiveness of follicular output rate, ovarian sensitivity index, and ovarian response prediction index for the ovarian reserve and response of low-prognosis patients according to the POSEIDON criteria: a retrospective study.","authors":"Zhilan Chen, Wei Li, Shufang Ma, Yanmin Li, Liqun Lv, Kecheng Huang, Aidong Gong","doi":"10.1017/S0967199423000382","DOIUrl":"10.1017/S0967199423000382","url":null,"abstract":"The aim was to explore the implications of follicular output rate (FORT), ovarian sensitivity index (OSI), ovarian response prediction index (ORPI), and follicle-to-oocyte index (FOI) in low-prognosis patients defined by POSEIDON criteria. In total, 4030 fresh in vitro fertilization (IVF) cycles from January 2013 to October 2021 were included in this retrospective cohort analysis and were categorized into four groups based on the POSEIDON criteria. The FORT between Groups 1 and 2 (0.61 ± 0.34 vs. 0.65 ± 0.35, P = 0.081) and Groups 3 and 4 (1.08 ± 0.82 vs. 1.09 ± 0.94, P = 0.899) were similar. The OSI in the order from the highest to the lowest were 3.01 ± 1.46 in Group 1, 2.28 ± 1.09 in Group 2, 1.54 ± 1.04 in Group 3, and 1.34 ± 0.96 in Group 4 (P < 0.001). The trend in the ORPI values was consistent with that in the OSI. FORT, OSI, ORPI, and FOI complemented each other and offered excellent effectiveness in reflecting ovarian reserve and response, but they were not good predictors of clinical pregnancy rate (CPR) from IVF.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum anti-Müllerian hormone concentrations are related to embryo development: lessons from time-lapse imaging. 血清抗米勒激素浓度与胚胎发育有关：延时成像的经验教训。

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-01 Epub Date: 2023-09-25 DOI: 10.1017/S0967199423000370

Amanda Souza Setti, Daniela Paes de Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Edson Borges

{"title":"Serum anti-Müllerian hormone concentrations are related to embryo development: lessons from time-lapse imaging.","authors":"Amanda Souza Setti, Daniela Paes de Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Edson Borges","doi":"10.1017/S0967199423000370","DOIUrl":"10.1017/S0967199423000370","url":null,"abstract":"Our objective was to study whether serum anti-Müllerian hormone (AMH) concentrations were associated with embryo morphokinetic events. This retrospective cohort study was performed in a private university-affiliated in vitro fertilization centre between March 2019 and December 2020 and included 902 oocytes cultured in a time-lapse imaging incubator, obtained from 114 intracytoplasmic sperm injection cycles performed. The relationship between AMH concentrations and morphokinetic events was investigated by considering the clustering of data (multiple embryos/patient). Evaluated kinetic markers were time to pronuclei appearance (tPNa) and fading (tPNf), time to two (t2), three (t3), four (t4), five (t5), six (t6), seven (t7), and eight cells (t8), (tSB) and time to the start of blastulation (tSB) and to blastulation (tB). Significant inverse relationships were observed between serum AMH concentrations and tPNf, t3, t4, t5, t6, t7, t8, and tB. The AMH was positively correlated with the KIDScore and implantation rate. Increased serum AMH concentrations correlated with faster embryo development. The clinical implications of this effect on embryo development warrant further investigation.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of trophectoderm markers in domestic cat blastocysts cultured without zona pellucida. 家猫无透明带囊胚营养外胚层标记物分析。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-08-31 DOI: 10.1017/S096719942200034X

Daniel Veraguas-Dávila, Darling Saéz-Ruíz, María Consuelo Álvarez, Fernando Saravia, Fidel Ovidio Castro, Lleretny Rodríguez-Alvarez

{"title":"Analysis of trophectoderm markers in domestic cat blastocysts cultured without zona pellucida.","authors":"Daniel Veraguas-Dávila, Darling Saéz-Ruíz, María Consuelo Álvarez, Fernando Saravia, Fidel Ovidio Castro, Lleretny Rodríguez-Alvarez","doi":"10.1017/S096719942200034X","DOIUrl":"https://doi.org/10.1017/S096719942200034X","url":null,"abstract":"Domestic cat embryos generated by in vitro fertilization (IVF) and cultured without the zona pellucida have a reduced implantation capacity after embryo transfer at the blastocyst stage. The objective of this study was to evaluate the expression of trophectoderm markers in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were selected: (1) domestic cat embryos generated by IVF and cultured in vitro normally (zona intact group, ZI); and (2) domestic cat embryos generated by IVF and cultured in vitro without a zona pellucida (zona-free group, ZF). In the ZF group, the zona pellucida of the presumptive zygote was removed and these were cultured using the well of the well (WOW) system. In vitro culture was carried out for 7 days. The cleavage, morula and blastocyst rates were estimated. Finally, the relative expression levels of the trophectoderm markers TEAD4, YAP1, CDX2 and EOMES, the cell adhesion marker E-cadherin and the apoptosis marker CASP3 were evaluated by RT-qPCR in the blastocysts. The Wilcoxon test was used to evaluate differences (P < 0.05). No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups. No differences were found in the expression of TEAD4, CDX2, E-cadherin and CASP3 between groups. The expression of YAP1 and EOMES was higher in ZF blastocysts than in ZI blastocysts. In conclusion, the in vitro culture without the zona pellucida generates an overexpression of YAP1 and EOMES in the domestic cat blastocysts. More studies are needed to confirm if this overexpression might affect in vivo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40333870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An additional medium renewal of D4 embryo culture improves the concordance of noninvasive chromosome screening with trophectoderm biopsy. D4胚胎培养的额外培养基更新提高了无创染色体筛查与滋养外胚层活检的一致性。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-22 DOI: 10.1017/S0967199422000363

Hong-Xing Li, Yan Pang, Xue-Hong Zhang, Di Cao, Xiao-Ling Ma

引用次数: 0

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