IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-04 DOI: 10.1017/S0967199423000576

Paola Maria da Silva Rosa, Pedro Henrique Evagelista Guedes, Joaquim Mansano Garcia, Clara Slade Oliveira

{"title":"Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.","authors":"Paola Maria da Silva Rosa, Pedro Henrique Evagelista Guedes, Joaquim Mansano Garcia, Clara Slade Oliveira","doi":"10.1017/S0967199423000576","DOIUrl":"10.1017/S0967199423000576","url":null,"abstract":"Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus-oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"28-37"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138478669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration. 使用传统吸管和无抽吸卵母细胞吸管进行卵胞浆内精子注射的比较研究。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2024-01-04 DOI: 10.1017/S0967199423000618

Francisco Vergara, Javier Fernández, Concepción Pedrosa, María Muñoz, Elvira Jerez, Mireia Varón, Carmen Moyano, Alberto Yoldi, Jordi Ponce

{"title":"Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration.","authors":"Francisco Vergara, Javier Fernández, Concepción Pedrosa, María Muñoz, Elvira Jerez, Mireia Varón, Carmen Moyano, Alberto Yoldi, Jordi Ponce","doi":"10.1017/S0967199423000618","DOIUrl":"10.1017/S0967199423000618","url":null,"abstract":"Despite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical-biological disciplines. In view of the above caveats, the aim of the study was to compare the results of ICSI with the traditional oocyte-holding pipette and the oocyte-holding pipette without aspiration (PiWA). In total, 155 patients and 1037 oocytes were included in the study. In each ICSI cycle, half of the oocytes were microinjected using a traditional holding pipette and the other half using a PiWA. In result, the PiWA technique produced a significant increase in the fertilization rate: 88.12% (95%CI: 84.62-90.92%); holding pipette: 73.33% (95%CI: 68.72-77.49%). Also, it produced a significant decrease in the embryo degeneration rate compared with the traditional holding pipette [PiWA: 2.07% (95%CI: 1.11-3.8%); holding pipette: 4.51% (95%CI: 3.06-6.59%)]. Pregnancy rate depended on the holding technique used, both in single embryo transfers (n = 59; χ2 = 4.608; P-value = 0.032) and double embryo transfers (n = 156; χ2 = 4.344; P-value = 0.037); with PiWA presenting a significantly higher pregnancy rate than the traditional holding technique. Based on current evidence and the present results, improvements should focus on decreasing the invasiveness of the microinjection itself by minimizing or avoiding aspiration and cytoplasmic disorganization, as is successfully achieved with PiWA.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"96-101"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during in vitro culture. 体外培养过程中辅酶Q10对小鼠卵泡线粒体生物发生的影响。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-04 DOI: 10.1017/S0967199423000461

Roya Harsini, Saeed Zavareh, Meysam Nasiri, Sara Seyfi

{"title":"The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during in vitro culture.","authors":"Roya Harsini, Saeed Zavareh, Meysam Nasiri, Sara Seyfi","doi":"10.1017/S0967199423000461","DOIUrl":"10.1017/S0967199423000461","url":null,"abstract":"The aim of this research was to investigate the effect of Coenzyme Q10 (CoQ10) on the expression of the Transcription Factor A Mitochondrial (Tfam) gene and mtDNA copy number in preantral follicles (PFs) of mice during in vitro culture. To conduct this experimental study, PFs were isolated from 14-day-old National Medical Research Institute mice and cultured in the presence of 50 µm CoQ10 for 12 days. On the 12th day, human chorionic gonadotropin was added to stimulate ovulation. The fundamental parameters, including preantral follicle developmental rate and oocyte maturation, were evaluated. Additionally, the Tfam gene expression and mtDNA copy number of granulosa cells and oocytes were assessed using the real-time polymerase chain reaction. The results revealed that CoQ10 significantly increased the diameter of PFs, survival rate, antrum formation, and metaphase II (MII) oocytes (P < 0.05). Moreover, in the CoQ10-treated groups, the Tfam gene expression in granulosa cells and oocytes increased considerably compared with the control group. The mtDNA copy number of granulosa cells and oocytes cultured in the presence of CoQ10 was substantially higher compared with the control groups (P < 0.05). The addition of CoQ10 to the culture medium enhances the developmental competence of PFs during in vitro culture by upregulating Tfam gene expression and increasing mtDNA copy number in oocyte and granulosa cells.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"14-20"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138478670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Raman spectroscopy to the evaluation of F-actin changes in sea urchin eggs at fertilization. 应用拉曼光谱评价海胆卵受精时f -肌动蛋白的变化。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-05 DOI: 10.1017/S0967199423000552

Maria Mangini, Nunzia Limatola, Maria Antonietta Ferrara, Giuseppe Coppola, Jong Tai Chun, Anna Chiara De Luca, Luigia Santella

{"title":"Application of Raman spectroscopy to the evaluation of F-actin changes in sea urchin eggs at fertilization.","authors":"Maria Mangini, Nunzia Limatola, Maria Antonietta Ferrara, Giuseppe Coppola, Jong Tai Chun, Anna Chiara De Luca, Luigia Santella","doi":"10.1017/S0967199423000552","DOIUrl":"10.1017/S0967199423000552","url":null,"abstract":"The actin filaments on the surface of echinoderm oocytes and eggs readily undergo massive reorganization during meiotic maturation and fertilization. In sea urchin eggs, the actin cytoskeletal response to the fertilizing sperm is fast enough to accompany Ca2+ signals and to guide sperm's entry into the egg. Although recent work using live cell imaging technology confirmed changes in the actin polymerization status in fertilized eggs, as was previously shown using light and electron microscopy, it failed to provide experimental evidence of F-actin depolymerization a few seconds after insemination, which is concurrent with the sperm-induced Ca2+ release. In the present study, we applied Raman microspectroscopy to tackle this issue by examining the spectral profiles of the egg's subplasmalemmal regions before and after treating the eggs with actin drugs or fertilizing sperm. At both early (15 s) and late (15 min) time points after fertilization, specific peak shifts in the Raman spectra revealed change in the actin structure, and Raman imaging detected the cytoskeletal changes corresponding to the F-actin reorganization visualized with LifeAct-GFP in confocal microscopy. Our observation suggests that the application of Raman spectroscopy, which does not require microinjection of fluorescent probes and exogenous gene expression, may serve as an alternative or even advantageous method in disclosing rapid subtle changes in the subplasmalemmal actin cytoskeleton that are difficult to resolve.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"38-48"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138483127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors. Kisspeptin在体外通过同源受体刺激绵羊卵巢卵泡发育。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-07 DOI: 10.1017/S096719942300059X

B Divya Sri, S Harsha Lekha, K Narendra Gopal Reddy, Deepa Pathipati, B Rambabu Naik, P Jagapathy Ramayya, K Veera Bramhaiah, L S S Varaprasad Reddy, A V N Siva Kumar

{"title":"Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors.","authors":"B Divya Sri, S Harsha Lekha, K Narendra Gopal Reddy, Deepa Pathipati, B Rambabu Naik, P Jagapathy Ramayya, K Veera Bramhaiah, L S S Varaprasad Reddy, A V N Siva Kumar","doi":"10.1017/S096719942300059X","DOIUrl":"10.1017/S096719942300059X","url":null,"abstract":"The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the in vitro development of preantral follicles (PFs) and (2) evolution of KP receptor gene (KISS1R) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0-100 µg/ml) in the culture medium of PFs for 6 days. The cumulus-oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development in vitro. The expression of the kisspeptin receptor gene at each development stages of in vivo developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and in vitro cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The KISS1R gene was expressed in follicular cells and oocytes at all the development stages of both in vivo developed and in vitro cultured follicles. Higher KISS1R gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"49-57"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural study: in vitro and in vivo differentiation of mice spermatogonial stem cells. 超微结构研究：小鼠精原干细胞的体外和体内分化。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-27 DOI: 10.1017/S096719942300062X

Zahra Bashiri, Mansoureh Movahedin, Vahid Pirhajati, Hamidreza Asgari, Morteza Koruji

{"title":"Ultrastructural study: in vitro and in vivo differentiation of mice spermatogonial stem cells.","authors":"Zahra Bashiri, Mansoureh Movahedin, Vahid Pirhajati, Hamidreza Asgari, Morteza Koruji","doi":"10.1017/S096719942300062X","DOIUrl":"10.1017/S096719942300062X","url":null,"abstract":"Mouse testicular tissue is composed of seminiferous tubules and interstitial tissue. Mammalian spermatogenesis is divided into three stages: spermatocytogenesis (mitotic divisions) in which spermatogonial stem cells (SSCs) turn into spermatocytes, followed by two consecutive meiotic divisions in which spermatocytes form spermatids. Spermatids differentiate into spermatozoa during spermiogenesis. Various factors affect the process of spermatogenesis and the organization of cells in the testis. Any disorder in different stages of spermatogenesis will have negative effects on male fertility. The aim of the current study was to compare the in vitro and in vivo spermatogenesis processes before and after transplantation to azoospermic mice using ultrastructural techniques. In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitro culture are similar to those of in vivo spermatogenesis, indicating that spermatogenic cell nature is unaltered in vitro.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"87-95"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women. 结构和代谢积层细胞的改变会影响体重不足妇女的卵母细胞质量。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-12-22 DOI: 10.1017/S0967199423000588

Hong Ji, Qing Zhang, Lu Ding, Rongjuan Chen, Fu Liu, Ping Li

{"title":"Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women.","authors":"Hong Ji, Qing Zhang, Lu Ding, Rongjuan Chen, Fu Liu, Ping Li","doi":"10.1017/S0967199423000588","DOIUrl":"10.1017/S0967199423000588","url":null,"abstract":"This study aimed to investigate the structural and metabolic changes in cumulus cells of underweight women and their effects on oocyte maturation and fertilization. The cytoplasmic ultrastructure was analyzed by electron microscopy, mitochondrial membrane potential by immunofluorescence, and mitochondrial DNA copy number by relative quantitative polymerase chain reaction. The expression of various proteins including the oxidative stress-derived product 4-hydroxynonenal (4-HNE) and autophagy and apoptosis markers such as Vps34, Atg-5, Beclin 1, Lc3-I, II, Bax, and Bcl-2 was assessed and compared between groups. Oocyte maturation and fertilization rates were lower in underweight women (P < 0.05), who presented with cumulus cells showing abnormal mitochondrial morphology and increased cell autophagy. Compared with the mitochondrial DNA copies of the control group, those of the underweight group increased but not significantly. The mitochondrial membrane potential was similar between the groups (P = 0.8). Vps34, Atg-5, Lc3-II, Bax, and Bcl-2 expression and 4-HNE levels were higher in the underweight group compared with the control group (P < 0.01); however, the Bax/Bcl-2 ratio was lower in the underweight group compared with the control group (P = 0.031). Additionally, Beclin 1 protein levels were higher in the underweight group compared with the control group but without statistical significance. In conclusion, malnutrition and other conditions in underweight women may adversely affect ovulation, and the development, and fertilization of oocytes resulting from changes to the intracellular structure of cumulus cells and metabolic processes. These changes may lead to reduced fertility or unsatisfactory reproduction outcomes in women.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"77-86"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138831939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos. 父亲高脂肪饮食改变着床前胚胎H3K36me3模式。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-11-29 DOI: 10.1017/S0967199423000448

Bin Meng, Jiahui He, Wenbin Cao, Yanru Zhang, Jia Qi, Shiwei Luo, Chong Shen, Juan Zhao, Ying Xue, Pengxiang Qu, Enqi Liu

{"title":"Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos.","authors":"Bin Meng, Jiahui He, Wenbin Cao, Yanru Zhang, Jia Qi, Shiwei Luo, Chong Shen, Juan Zhao, Ying Xue, Pengxiang Qu, Enqi Liu","doi":"10.1017/S0967199423000448","DOIUrl":"10.1017/S0967199423000448","url":null,"abstract":"The global transition towards diets high in calories has contributed to 2.1 billion people becoming overweight, or obese, which damages male reproduction and harms offspring. Recently, more and more studies have shown that paternal exposure to stress closely affects the health of offspring in an intergenerational and transgenerational way. SET Domain Containing 2 (SETD2), a key epigenetic gene, is highly conserved among species, is a crucial methyltransferase for converting histone 3 lysine 36 dimethylation (H3K36me2) into histone 3 lysine 36 trimethylation (H3K36me3), and plays an important regulator in the response to stress. In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) male mice fed a HFD were traceable in SETD2/H3K36me3 in embryos, and that a paternal high-fat diet brings about adverse effects for offspring that might be related to SETD2/H3K36me3, which throws new light on the effect of paternal obesity on offspring from an epigenetic perspective.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138452700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment. 辅助生殖治疗中不同程度透明带异常卵母细胞的胚胎学特征及临床结局。

IF 1.7 4区生物学

Zygote Pub Date : 2024-02-01 Epub Date: 2023-11-29 DOI: 10.1017/S0967199423000515

Junshun Fang, Hua Sun, Linjun Chen, Jie Wang, Fei Lin, Zhipeng Xu, Lihua Zhu, Shanshan Wang

{"title":"Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment.","authors":"Junshun Fang, Hua Sun, Linjun Chen, Jie Wang, Fei Lin, Zhipeng Xu, Lihua Zhu, Shanshan Wang","doi":"10.1017/S0967199423000515","DOIUrl":"10.1017/S0967199423000515","url":null,"abstract":"Abnormalities in the zona pellucida (ZP) adversely affect oocyte maturation, embryo development and pregnancy outcomes. However, the assessment of severity is challenging. To evaluate the effects of different degrees of ZP abnormalities on embryo development and clinical outcomes, in total, 590 retrieval cycles were scored and divided into four categories (control, mild, moderate and severe) based on three parameters: perivitelline space, percentage of immature oocytes and percentage of oocytes with abnormal morphology. As the severity of abnormal ZP increased, both the number of retrieved oocytes and mature oocytes decreased. The fertilization rate did not differ significantly among groups. The rates of embryo cleavage and day-3 high-quality embryos in the mild group and the moderate group did not vary significantly between the two groups but were significantly higher than those in the severe group. The blastulation rates of the abnormal ZP groups were similar; however, they were lower than those of the control group. Moreover, the cycle cancellation rate of the severe abnormal ZP group was as high as 66.20%, which was significantly higher than that of the other three groups. Although the rates of cumulative clinical pregnancy and live births were lower than those in the control group, they were comparable among the abnormal ZP groups. There were no differences in the neonatal outcomes of the different groups. Together, ZP abnormalities show various degrees of severity, and in all patients regardless of the degree of ZP abnormalities who achieve available embryos, there will be an opportunity to eventually give birth.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"7-13"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138452699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsA promotes trophoblast invasion accompanied by changes in leukaemic inhibitory factor and fibroblast growth factor in peri-implantation blastocysts CsA 可促进滋养细胞侵袭，并伴随着植入前囊胚中白血病抑制因子和成纤维细胞生长因子的变化

IF 1.7 4区生物学

Zygote Pub Date : 2023-12-21 DOI: 10.1017/s0967199423000497

Dan Li, Qiuling Jie, Qi Li, Ping Long, Zhen Wang, Jiaxing Wang, Shengnan Tian, Menglan Wu, Yanlin Ma, Yuanhua Huang

引用次数: 0

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