IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1017/S0967199422000338

Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro

{"title":"β-Mercaptoethanol in culture medium improves cryotolerance of in vitro-produced bovine embryos.","authors":"Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro","doi":"10.1017/S0967199422000338","DOIUrl":"https://doi.org/10.1017/S0967199422000338","url":null,"abstract":"The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine in vitro-produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME for 72 h. Embryos cultured in 100 μM βME attained higher hatching rates (66.7%) than those culture in 0 (47.7%) and 50 (52.4%) μM βME. In Experiment II, IVP embryos were in vitro-cultured (IVC) to the blastocyst stage in 0 (control) or 100 μM βME, followed by vitrification. After warming, embryos were cultured for 72 h (post-warming culture, PWC) in 0 (control) or 100 μM βME, in a 2 × 2 factorial design: (i) CTRL-CTRL, control IVC and control PWC; (ii) CTRL-βME, control IVC and βME-supplemented PWC; (iii) βME-CTRL, βME-supplemented IVC and control PWC; or (iv) βME-βME, βME-supplemented IVC and βME-supplemented PWC. βME during IVC reduced embryo development (28.0% vs. 43.8%) but, following vitrification, higher re-expansion rates were seen in βME-CTRL (84.0%) and βME-βME (87.5%) than in CTRL-CTRL (71.0%) and CTRL-βME (73.1%). Hatching rates were higher in CTRL-βME (58.1%) and βME-βME (63.8%) than in CTRL-CTRL (36.6%) and βME-CTRL (42.0%). Total cell number in hatched blastocysts was higher in βME-βME (181.2 ± 7.4 cells) than CTRL-CTRL (139.0 ± 9.9 cells). Adding βME to the IVC medium reduced development but increased cryotolerance, whereas adding βME to the PWC medium improved embryo survival, hatching rates, and total cell numbers.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33477713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

RA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos. 白藜芦醇类似物RA33对体外受精牛胚胎的发育有促进作用。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1017/S0967199422000430

Tais Aparecida Patrocinio, Carlos Antônio de Carvalho Fernandes, Gilson Costa Macedo, Adilson David da Silva, Nadia Rezende Raposo Barbosa, Carolina Capobiango Romano Quintao, Marcela Eduarda da Silva Ribeiro, Luiz Sergio Almeida Camargo

{"title":"RA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos.","authors":"Tais Aparecida Patrocinio, Carlos Antônio de Carvalho Fernandes, Gilson Costa Macedo, Adilson David da Silva, Nadia Rezende Raposo Barbosa, Carolina Capobiango Romano Quintao, Marcela Eduarda da Silva Ribeiro, Luiz Sergio Almeida Camargo","doi":"10.1017/S0967199422000430","DOIUrl":"https://doi.org/10.1017/S0967199422000430","url":null,"abstract":"Oxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro-fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher (P < 0.05) with 2.5 μM (69.0 ± 4.4%) than at 0, 0.1 or 0.5 μM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 μM RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher (P < 0.05) than for 0 μM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 μM RA33 was compared with 0.5 μM resveratrol, similar (P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher (P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower (P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro-fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33479737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boar semen cryopreserved with trehalose-containing liposomes: disaccharide determination and rheological behaviour. 用含海藻糖脂质体冷冻保存的猪精液:双糖测定和流变行为。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-15 DOI: 10.1017/S0967199422000442

Claudia Denisse Mendoza-Viveros, Oscar Gutiérrez-Pérez, María Josefa Bernad-Bernad, Luis Medina-Torres, Minerva Monroy-Barreto, Miquel Gimeno, María Elena Trujillo-Ortega

{"title":"Boar semen cryopreserved with trehalose-containing liposomes: disaccharide determination and rheological behaviour.","authors":"Claudia Denisse Mendoza-Viveros, Oscar Gutiérrez-Pérez, María Josefa Bernad-Bernad, Luis Medina-Torres, Minerva Monroy-Barreto, Miquel Gimeno, María Elena Trujillo-Ortega","doi":"10.1017/S0967199422000442","DOIUrl":"https://doi.org/10.1017/S0967199422000442","url":null,"abstract":"This study aimed to detect intracellular trehalose in boar sperm that were cryopreserved with liposomes and conduct an analysis of its effects on some characteristics of thawed sperm, including rheological properties. First, soybean lecithin cholesterol-based liposomes were produced and characterized in the presence of 300 mM trehalose. Next, semen samples were frozen in two freezing media: a control medium with 300 mM trehalose and an experimental medium supplemented with 300 mM trehalose and 10% liposomes, both of which were thawed and then studied to ascertain their integrity, motility, rheological response, and trehalose quantities by testing two methods of spermatic lysis via high-performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD). The results found spherical liposomes measuring 357 nm that were relatively stable in an aqueous medium and had an entrapment efficiency of 73%. An analysis of the cryopreserved ejaculates showed that their viability and motility did not significantly differ between groups (P > 0.05). The viscous response of the samples was influenced by the extracellular medium rather than by the freezing-thawing process, which resulted in a loss of interaction between the cells and cryoprotectants. Finally, intracellular trehalose levels were determined using HPLC-ELSD, with no differences observed (P > 0.05) when comparing both sperm lysis methods. The use of liposomes with trehalose appears to be a promising option for boar semen cryopreservation, with a marked effect on rheological properties. The proposed HPLC-ELSD method was effective for measuring trehalose in cryopreserved cell samples.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Supplementation of porcine in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality. 在猪体外成熟培养基中添加FGF2、LIF和IGF1，可促进青春期前后备母猪卵母细胞细胞质成熟，提高胚胎质量。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-01 DOI: 10.1017/S0967199422000284

María Serrano Albal, Giuseppe Silvestri, Lucas G Kiazim, Lucy M Vining, Louisa J Zak, Grant A Walling, Alexandra M Haigh, Simon C Harvey, Katie E Harvey, Darren K Griffin

{"title":"Supplementation of porcine in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality.","authors":"María Serrano Albal, Giuseppe Silvestri, Lucas G Kiazim, Lucy M Vining, Louisa J Zak, Grant A Walling, Alexandra M Haigh, Simon C Harvey, Katie E Harvey, Darren K Griffin","doi":"10.1017/S0967199422000284","DOIUrl":"https://doi.org/10.1017/S0967199422000284","url":null,"abstract":"In porcine in vitro production (IVP) systems, the use of oocytes derived from prepubertal gilts, whilst being commercially attractive, remains challenging due to their poor developmental competence following in vitro maturation (IVM). Follicular fluid contains important growth factors and plays a key role during oocyte maturation; therefore, it is a common supplementation for porcine IVM medium. However, follicular fluid contains many poorly characterized components, is batch variable, and its use raises biosecurity concerns. In an effort to design a defined IVM system, growth factors such as cytokines have been previously tested. These include leukaemia inhibitory factor (LIF), fibroblast growth factor 2 (FGF2), and insulin-like growth factor 1 (IGF1), the combination of which is termed 'FLI'. Here, using abattoir-derived oocytes in a well established porcine IVP system, we compared follicular fluid and FLI supplementation during both IVM and embryo culture to test the hypothesis that FLI can substitute for follicular fluid without compromising oocyte nuclear and cytoplasmic maturation. We demonstrate that in oocytes derived from prepubertal gilts, FLI supplementation enhances oocyte meiotic maturation and has a positive effect on the quality and developmental competence of embryos. Moreover, for the first time, we studied the effects of follicular fluid and FLI combined showing no synergistic effects.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40336493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dynamic and aberrant patterns of H3K4me3, H3K9me3, and H3K27me3 during early zygotic genome activation in cloned mouse embryos. 克隆小鼠胚胎早期合子基因组激活过程中H3K4me3、H3K9me3和H3K27me3的动态和异常模式

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-15 DOI: 10.1017/S0967199422000454

Zhihui Liu, Jing Cui, Weiguo Wang, Mingyang Li, Zhisong Wang, Giorgio Antonio Presicce, Xiuchun Cindy Tian, Liyou An, Fuliang Du

{"title":"Dynamic and aberrant patterns of H3K4me3, H3K9me3, and H3K27me3 during early zygotic genome activation in cloned mouse embryos.","authors":"Zhihui Liu, Jing Cui, Weiguo Wang, Mingyang Li, Zhisong Wang, Giorgio Antonio Presicce, Xiuchun Cindy Tian, Liyou An, Fuliang Du","doi":"10.1017/S0967199422000454","DOIUrl":"https://doi.org/10.1017/S0967199422000454","url":null,"abstract":"Somatic cell nuclear transfer (NT) is associated with aberrant changes in epigenetic reprogramming that impede the development of embryos, particularly during zygotic genome activation. Here, we characterized epigenetic patterns of H3K4me3, H3K9me3, and H3K27me3 in mouse NT embryos up to the second cell cycle (i.e. four-celled stage) during zygotic genome activation. In vivo fertilized and parthenogenetically activated (PA) embryos served as controls. In fertilized embryos, maternal and paternal pronuclei exhibited asymmetric H3K4me3, H3K9me3, and H3K27me3 modifications, with the paternal pronucleus showing delayed epigenetic modifications. Higher levels of H3K4me3 and H3K9me3 were observed in NT and PA embryos than in fertilized embryos. However, NT embryos exhibited a lower level of H3K27me3 than PA and fertilized embryos from pronuclear stage 3 to the four-celled stage. Our finding that NT embryos exhibited aberrant H3K4me3, H3K9me3, and H3K27me3 modifications in comparison with fertilized embryos during early zygotic genome activation help to unravel the epigenetic mechanisms of methylation changes in early NT reprogramming and provide an insight into the role of histone H3 in the regulation of cell plasticity during natural reproduction and somatic cell NT.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Strategy for embryo transfer to improve pregnancy outcomes in advanced maternal age. 胚胎移植改善高龄产妇妊娠结局的策略。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-07-21 DOI: 10.1017/S0967199422000259

Xue Wang, Yaling Xiao, Zhengyi Sun, Jingran Zhen, Qi Yu

{"title":"Strategy for embryo transfer to improve pregnancy outcomes in advanced maternal age.","authors":"Xue Wang, Yaling Xiao, Zhengyi Sun, Jingran Zhen, Qi Yu","doi":"10.1017/S0967199422000259","DOIUrl":"https://doi.org/10.1017/S0967199422000259","url":null,"abstract":"The purpose of this retrospective study was to optimize the transplantation strategy for women of advanced maternal age to achieve live births within the shortest time. Data were collected from patients older than 40 years who underwent assisted reproductive therapy at our centre from 1 January 2009 to 31 December 2019. In total, 1023 cases of fresh cleavage embryo transfer (CET) cycles, 280 cases of frozen-thawed blastocyst transfer (FBT) cycles, and 26 cases of frozen-thawed CET (FCET) cycles were included. The main outcome was the live birth rate (LBR). The secondary outcomes were the clinical pregnancy rate (CPR) and neonatal outcomes. Multivariable logistic regression was performed to adjust for confounding factors. The blastocyst formation rate of patients older than 40 years was 23.5%, the freezing cycle rate was 19.8%, and the fresh-embryo transfer rate was 83.0%. The implantation rate, CPR, and LBR were significantly different among the CET, FCET, and FBT groups. There were no significant differences in multiple pregnancies and abortion rates among the groups, and neonatal outcomes were similar. Multivariate logistic regression analysis showed that, compared with the CET group, LBR did not increase in the FCET group, whereas LBR increased in the FBT group. For patients older than 40 years when having approximately eight embryos after fertilization, blastocyst transfer can be considered after fully discussing the advantages and disadvantages of blastocyst culture. Alternatively, CET can be performed first, followed by FBT if the cleavage embryo transfer is unsuccessful.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40634285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid supplementation on maternal reproductive parameters. 补充顺式-9、反式-11和反式-10、顺式-12共轭亚油酸对母体生殖参数的影响

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1017/S0967199422000405

Danielle Storino de Freitas, Guilherme Antonio de Gouvêa Lopes, Barbara Rodrigues Nascimento, Breno Augusto Magalhães, Ana Paula Madureira, Paulo Henrique Almeida Campos-Junior

{"title":"Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid supplementation on maternal reproductive parameters.","authors":"Danielle Storino de Freitas, Guilherme Antonio de Gouvêa Lopes, Barbara Rodrigues Nascimento, Breno Augusto Magalhães, Ana Paula Madureira, Paulo Henrique Almeida Campos-Junior","doi":"10.1017/S0967199422000405","DOIUrl":"https://doi.org/10.1017/S0967199422000405","url":null,"abstract":"Conjugated linoleic acid (CLA) is a mixture of positional isomers of linoleic acid found in ruminant products and meat. The diet supplementing with CLA is an emerging area, requiring studies to elucidate its effects on animals and human reproduction, as well as its side effects. Therefore, the aim of this study was to evaluate the effects of CLA gastric administration, during the pregestational and gestational period in biometric and reproductive parameters, as well as in ovarian morphophysiology. Animals were distributed in three groups: (1) control (n = 10); (2) fish oil (n = 10); and (3) CLA (n = 10), that daily received, by gavage, phosphate-buffered saline, fish oil and CLA, respectively, carried out over 50 days (before mating, mating and pregnancy). There was an increment in the nasoanal distance and Lee index of the CLA and fish oil-treated groups during the first weeks (P > 0.05). CLA administration did not affect the ovarian follicle mobilization (P > 0.05), the number of follicles (P > 0.05) and the integrated density of lipid content of oocytes included in antral follicles (P > 0.05). There was no effect of CLA administration on the litter weight (P > 0.05; F2 and F3), however, an increment (P < 0.05) in the number of pups per litter (F2) was observed. Overall, this study demonstrated the absence of side effects of the CLA gastric administration on mice reproductive performance and suggests that this treatment would transgenerationally enhance fertility in this species.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33477675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microtubule-severing protein Fidgetin-like 1 promotes spindle organization during meiosis of mouse oocytes. 微管切断蛋白Fidgetin-like 1促进小鼠卵母细胞减数分裂过程中的纺锤体组织。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1017/S0967199422000417

Hua-Feng Shou, Zhen Jin, Yan Yu, Yu-Cheng Lai, Qing Wu, Lei-Lei Gao

引用次数: 2

Assessment of mitochondrial DNA viability ratio in day-4 biopsied embryos as an add-in to select euploid embryos for single embryo transfer. 评估第4天活检胚胎的线粒体DNA活力比，作为选择整倍体胚胎进行单胚胎移植的附加手段。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1017/S0967199422000260

Ahmad Metwalley, Ali Hellani, Azza A Abdelrazek, Ahmed El-Damen, Ahmed Al Dawood, Nabeel Brasha, Sandro C Esteves, Manal El Hamshary, Omaima Khamiss

{"title":"Assessment of mitochondrial DNA viability ratio in day-4 biopsied embryos as an add-in to select euploid embryos for single embryo transfer.","authors":"Ahmad Metwalley, Ali Hellani, Azza A Abdelrazek, Ahmed El-Damen, Ahmed Al Dawood, Nabeel Brasha, Sandro C Esteves, Manal El Hamshary, Omaima Khamiss","doi":"10.1017/S0967199422000260","DOIUrl":"https://doi.org/10.1017/S0967199422000260","url":null,"abstract":"The aim of this study was to assess mitochondrial DNA analysis as a predictor of the pregnancy potential of biopsied preimplantation embryos. The study included 78 blastomeres biopsied from day 4 cleavage stage euploid embryos. The embryo karyotype was confirmed by 24-chromosome preimplantation genetic testing for aneuploidies using the Illumina Next-Generation Sequencing (NGS) system. Mitochondria viability ratios (mtV) were determined from BAM files subjected to the web-based genome-analysis tool Galaxy. From this cohort of patients, 30.4% of patients (n = 34) failed to establish pregnancy. The mean mtV ratio [mean = 1.51 ± 1.25-1.77 (95% CI)] for this group was significantly (P < 0.01) lower compared with the embryo population that resulted in established pregnancies [mean = 2.5 ± 1.82-2.68 (95% CI)]. mtV multiple of mean (MoM) values were similarly significantly (P < 0.01) lower in blastocysts failing to establish pregnancy. At a 0.5 MoM cut-off, the sensitivity of mtV quantitation was 35.3% and specificity was 78.2%. The positive predictive value for an mtV value > 0.5 MoM was 41.4%. This study demonstrates the clinical utility of preimplantation quantification of viable mitochondrial DNA in biopsied blastomeres as a prognosticator of pregnancy potential.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33478266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival analysis for estimating lamb survival up to weaning in Harnali sheep. 哈纳利羊断奶前羔羊存活率分析。

IF 1.7 4区生物学

Zygote Pub Date : 2022-12-01 Epub Date: 2022-08-01 DOI: 10.1017/S0967199422000272

Parth Gaur, Z S Malik, Yogesh C Bangar, Ankit Magotra, Ashish Chauhan

{"title":"Survival analysis for estimating lamb survival up to weaning in Harnali sheep.","authors":"Parth Gaur, Z S Malik, Yogesh C Bangar, Ankit Magotra, Ashish Chauhan","doi":"10.1017/S0967199422000272","DOIUrl":"https://doi.org/10.1017/S0967199422000272","url":null,"abstract":"The present study was carried out to estimate lamb survival (in days) from birth to weaning under survival analysis using data records from 2057 Harnali lambs born to 134 sires and 623 dams between the period from 2001 to 2020. The weaning age in resourced population was 90 days from birth. The hazard ratio in terms of risk of death up to weaning was determined using Cox proportional hazards model by subjecting some fixed factors such as year of birth, sex of lamb, birth weight (kg), dam's weight at lambing (kg) and dam's age at lambing (years). The overall survivability up to weaning among lambs was 91.59% and Kaplan-Meier estimates of mean survival time up to weaning was 85.77 days. Cox proportional hazard modelling revealed that the hazards of death up to weaning was higher in male lambs [1.66, 95% confidence interval (CI): 1.22-2.26] compared with female lambs [hazard ratio (HR) = 1.00]. It was also observed that the hazards of death (HR = 0.91, 95% CI: 0.88-0.94) had decreasing trends over years. For birth weight (kg), hazard rate was 0.34 (95% CI: 0.25-0.46), which indicated that the risk of pre-weaning mortality was lower as birth weight increases. The weight and age of dams at lambing did not influence the survival time of studied population. The present findings indicated that survival time increased in studied lambs over the years and it could be increased more by giving more emphasis on better litter weight and general health aspects at farm level.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40574456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

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