IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000291

Chun-Hui Jin, Ren-Ren Chen, Xiu-Yun Feng, Jun-Gui Zhao, Ming-Tao Xu, Min Zhang, Jun-Zuo Wang, Jing-He Tan

{"title":"Effects of calcium-free ageing on ethanol-induced activation and developmental potential of mouse oocytes.","authors":"Chun-Hui Jin, Ren-Ren Chen, Xiu-Yun Feng, Jun-Gui Zhao, Ming-Tao Xu, Min Zhang, Jun-Zuo Wang, Jing-He Tan","doi":"10.1017/S0967199423000291","DOIUrl":"https://doi.org/10.1017/S0967199423000291","url":null,"abstract":"Although ethanol treatment is widely used to activate oocytes, the underlying mechanisms are largely unclear. Roles of intracellular calcium stores and extracellular calcium in ethanol-induced activation (EIA) of oocytes remain to be verified, and whether calcium-sensing receptor (CaSR) is involved in EIA is unknown. This study showed that calcium-free ageing (CFA) in vitro significantly decreased intracellular stored calcium (sCa) and CaSR expression, and impaired EIA, spindle/chromosome morphology and developmental potential of mouse oocytes. Although EIA in oocytes with full sCa after ageing with calcium does not require calcium influx, calcium influx is essential for EIA of oocytes with reduced sCa after CFA. Furthermore, the extremely low EIA rate in oocytes with CFA-downregulated CaSR expression and the fact that inhibiting CaSR significantly decreased the EIA of oocytes with a full complement of CaSR suggest that CaSR played a significant role in the EIA of ageing oocytes. In conclusion, CFA impaired EIA and the developmental potential of mouse oocytes by decreasing sCa and downregulating CaSR expression. Because mouse oocytes routinely treated for activation (18 h post hCG) are equipped with a full sCa complement and CaSR, the present results suggest that, while calcium influx is not essential, CaSR is required for the EIA of oocytes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"393-401"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9793252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sibling oocytes cultured in a time-lapse versus benchtop incubator: how time-lapse incubators improve blastocyst development and euploid rate. 延时培养与台式培养的同胞卵母细胞:延时培养如何提高囊胚发育和整倍体率

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000242

N G Nobrega, A Abdala, A El-Damen, A Arnanz, A Bayram, I Elkhatib, B Lawrenz, H Fatemi, N De Munck

{"title":"Sibling oocytes cultured in a time-lapse versus benchtop incubator: how time-lapse incubators improve blastocyst development and euploid rate.","authors":"N G Nobrega, A Abdala, A El-Damen, A Arnanz, A Bayram, I Elkhatib, B Lawrenz, H Fatemi, N De Munck","doi":"10.1017/S0967199423000242","DOIUrl":"https://doi.org/10.1017/S0967199423000242","url":null,"abstract":"The aim was to study whether a limited exposure of embryos outside the incubator has an effect on embryo development, blastocyst quality and euploid outcomes. This retrospective study was performed at ART Fertility Clinics, Abu Dhabi, United Arab Emirates (UAE) between March 2018 and April 2020 and included 796 mature sibling oocytes that were split randomly between two incubators after intracytoplasmic sperm injection (ICSI): an EmbryoScope™ (ES) incubator and a benchtop incubator, G185 K-SYSTEMS (KS). The fertilization, cleavage, embryo/blastocyst qualities, useable blastocyst and euploid rates were assessed to evaluate the incubator performance. In total, 503 (63.2%) mature oocytes were cultured in the EmbryoScope and 293 (36.8%) in the K-SYSTEMS. No differences were observed in fertilization rate (79.3% vs 78.8%, P = 0.932), cleavage rate (98.5% vs 99.1%, P = 0.676) and embryo quality on Day 3 (P = 0.543) between both incubators, respectively. Embryos cultured in the EmbryoScope, had a significantly higher chance of being biopsied (64.8% vs 49.6%, P < 0.001). Moreover, a significantly higher blastocyst biopsy rate was observed on Day 5 in the EmbryoScope (67.8% vs 57.0%, P = 0.037), with a highly significant increased euploid rate (63.5% vs 37.4%, P = 0.001) and improved blastocyst quality (P = 0.008). We found that exposure of embryos outside the incubator may negatively affect the in vitro blastocyst development and euploid rate on Day 5.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"402-409"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9793801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pum3 is dispensable for mouse oocyte maturation and embryo development in vitro. Pum3是小鼠卵母细胞体外成熟和胚胎发育所必需的。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000205

TingTing Zhao, Wei Huang, Kaibo Lin

{"title":"Pum3 is dispensable for mouse oocyte maturation and embryo development in vitro.","authors":"TingTing Zhao, Wei Huang, Kaibo Lin","doi":"10.1017/S0967199423000205","DOIUrl":"https://doi.org/10.1017/S0967199423000205","url":null,"abstract":"Pumilio3 (Pum3), an evolutionarily distant homologue of the classical RNA-binding protein PUF (PUMILIO and FBF) family member, is also involved in the process of RNA metabolism through post-transcriptional regulation. However, the functions of Pum3 in mouse oocyte maturation and preimplantation embryonic development have not been elucidated. By comparing RNA levels in different tissues, we found that Pum3 was widely expressed in multiple tissues, but moderately predominant in the ovary. Histochemical staining suggested that the PUM3 protein exhibits positive signals in oocytes, granulosa cells and theca cells of different follicle stages. Oocyte immunofluorescence results showed a slightly higher level of PUM3 protein in metaphase II compared with the germinal vesicle (GV) stage. After knockdown of Pum3 in GV oocytes using siRNA injection (siPUM3), no obvious defect was observed in the processes of GV breakdown and polar body extrusion during in vitro maturation (IVM) for the siPum3 oocytes. Compared with the control group, the siPUM3 group displayed no significant abnormality in the cleavage and blastocyst formation rate of these fertilized oocytes. Therefore, we can conclude that depletion of Pum3 does not affect mouse oocyte maturation and early embryonic development in vitro.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"359-365"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10151780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of serum progesterone levels on the day of frozen embryo transfers according to type of endometrial preparation: a single centre, retrospective study. 根据子宫内膜制备类型，冷冻胚胎移植当天血清孕酮水平的比较:单中心回顾性研究。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000163

Olivier Pouget, Zakarya Zemmache, Sarah Kabani, Malak Alsawaf, Irma Zuna, Marine Bonneau, Marie Laure Tailland, Julie Nobre Meirinhos, Nathalie Rougier-Maillard, Julien Sigala, Sophie Poirey, Stéphanie Huberlant

{"title":"Comparison of serum progesterone levels on the day of frozen embryo transfers according to type of endometrial preparation: a single centre, retrospective study.","authors":"Olivier Pouget, Zakarya Zemmache, Sarah Kabani, Malak Alsawaf, Irma Zuna, Marine Bonneau, Marie Laure Tailland, Julie Nobre Meirinhos, Nathalie Rougier-Maillard, Julien Sigala, Sophie Poirey, Stéphanie Huberlant","doi":"10.1017/S0967199423000163","DOIUrl":"https://doi.org/10.1017/S0967199423000163","url":null,"abstract":"The live birth rate following embryo transfer is comparable between spontaneous, stimulated and artificial cycles. However, the pregnancy loss rate appears elevated with hormonal therapy, possibly due to luteal insufficiency. This study aimed to determine whether the serum progesterone level on transfer day differed according to the endometrial preparation method for frozen embryo transfer (FET). Twenty spontaneous cycles (SC), 27 ovarian stimulation cycles (OS) and 65 artificial cycles (AC) were retrospectively studied from May to December 2019 in a single French hospital. The primary endpoint was the level of serum progesterone on the day of FET between the three endometrial preparation methods. The mean serum progesterone level on transfer day was 29.47 ng/ml in the OS group versus 20.03 ng/ml in the SC group and 14.32 ng/ml AC group (P < 0.0001). Progesterone levels remained significantly different after logistic regression on age and anti-Müllerian hormone (AMH) level. There was no significant difference in demographic and hormone characteristics (age, body mass index, embryo stage of embryo, type of infertility, basal follicle stimulating hormone, luteinizing hormone, estradiol and AMH levels), endometrial thickness, number and type of embryos transferred, duration of infertility, pregnancy rate, live birth rate and pregnancy loss rate. No difference was found in serum progesterone levels between clinical pregnancy with fetal heartbeat and no clinical pregnancy (no pregnancy or pregnancy loss, 17.49 ng/ml vs 20.83 ng/ml, respectively, P = 0.07). The lower serum progesterone level found on FET day in the AC group should be further investigated to see whether this difference has a clinical effect on the live birth rate.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"373-379"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanisms that control the preantral to early antral follicle transition and the strategies to have efficient culture systems to promote their growth in vitro. 控制腔前卵泡向早期腔前卵泡过渡的机制以及建立有效的体外培养系统促进其生长的策略。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000254

D R Nascimento, E C Barbalho, L Gondim Barrozo, E I T de Assis, F C Costa, J R V Silva

引用次数: 0

Sex steroid receptors in the ovarian follicles of the lizard Sceloporus torquatus. 蜥蜴卵巢卵泡内的性类固醇受体。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S096719942300028X

Norma Berenice Cruz-Cano, Uriel Ángel Sánchez-Rivera, Carmen Álvarez-Rodríguez, Mario Cárdenas-León, Martín Martínez-Torres

引用次数: 1

Autophagic activation in porcine oocytes is independent of meiotic progression. 猪卵母细胞的自噬激活与减数分裂进程无关。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000102

SeungHoon Lee, Haeyun Jeong, Hayeon Wi, Jin-Gu No, Whi-Cheul Lee, Seokho Kim, Hyeon Yang, Sung June Byun, Sejin Park, Jong Gug Kim

{"title":"Autophagic activation in porcine oocytes is independent of meiotic progression.","authors":"SeungHoon Lee, Haeyun Jeong, Hayeon Wi, Jin-Gu No, Whi-Cheul Lee, Seokho Kim, Hyeon Yang, Sung June Byun, Sejin Park, Jong Gug Kim","doi":"10.1017/S0967199423000102","DOIUrl":"https://doi.org/10.1017/S0967199423000102","url":null,"abstract":"In this study, we built on our previous research that discovered that autophagy activated the metaphase I stage during porcine oocytes in vitro maturation. We investigated the relationship between autophagy and oocyte maturation. First, we confirmed whether autophagy was activated differently by different media (TCM199 and NCSU-23) during maturation. Then, we investigated whether oocyte maturation affected autophagic activation. In addition, we examined whether the inhibition of autophagy affected the nuclear maturation rate of porcine oocytes. As for the main experiment, we measured LC3-II levels using western blotting after inhibition of nuclear maturation via cAMP treatment in an in vitro culture to clarify whether nuclear maturation affected autophagy. After autophagy inhibition, we also counted matured oocytes by treating them with wortmannin or a E64d and pepstatin A mixture. Both groups, which had different treatment times of cAMP, showed the same levels of LC3-II, while the maturation rates were about four times higher after cAMP 22 h treatment than that of the 42 h treatment group. This indicated that neither cAMP nor nuclear status affected autophagy. Autophagy inhibition during in vitro oocyte maturation with wortmannin treatment reduced oocyte maturation rates by about half, while autophagy inhibition by the E64d and pepstatin A mixture treatment did not significantly affect the oocyte maturation. Therefore, wortmannin itself, or the autophagy induction step, but not the degradation step, is involved in the oocyte maturation of porcine oocytes. Overall, we propose that oocyte maturation does not stand upstream of autophagy activation, but autophagy may exist upstream of oocyte maturation.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"380-385"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9793253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association between anti-Müllerian hormone levels and reproductive parameters in Wagyu cattle raised in Brazil. 巴西饲养的和牛抗<s:1>勒氏杆菌激素水平与生殖参数的关系。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000229

Flávia de Marchi, Renan Lazzaretti, Janine de Camargo, Fernanda Luiza Facioli, Eraldo Lourenso Zanella, Pedro Nacib Jorge-Neto, Mariana Groke Marques, Kyle Cody Caires, Ricardo Zanella

{"title":"Association between anti-Müllerian hormone levels and reproductive parameters in Wagyu cattle raised in Brazil.","authors":"Flávia de Marchi, Renan Lazzaretti, Janine de Camargo, Fernanda Luiza Facioli, Eraldo Lourenso Zanella, Pedro Nacib Jorge-Neto, Mariana Groke Marques, Kyle Cody Caires, Ricardo Zanella","doi":"10.1017/S0967199423000229","DOIUrl":"https://doi.org/10.1017/S0967199423000229","url":null,"abstract":"The production of in vitro embryos has sped up the dissemination of superior genetic material. However, the variation among the cattle response to oocyte and embryo production is a challenging factor. This variation is even higher in the Wagyu cattle as the breed has a small effective population size. The identification of an effective marker related to reproductive efficiency would allow the selection of more responsive females to reproductive protocols. The objective of this study was to evaluate the blood levels of anti-Müllerian hormone and associate it with oocyte recovery and blastocyst rate of embryos produced in vitro in Wagyu cows, as well as observe the hormone circulating levels in males. Serum samples from 29 females with seven follicular aspirations and four bulls were used. AMH measurements were performed using the bovine AMH ELISA kit. A positive correlation was identified between oocyte production and blastocyst rate (r = 0.84, P = 9 × 10-9), and AMH levels with oocyte (r = 0.49, P = 0.006) and embryo (r = 0.39, P = 0.03) production. The mean levels of AMH were different between animals with low (11.06 ± 3.01) and high (20.75 ± 4.46) oocyte production (P = 0.01). Males showed high serological levels of AMH (3829 ± 2328 pg/ml) compared with other breeds. It is possible to use the serological measurement of AMH as a method to select Wagyu females with greater capacity for oocyte and embryo production. Further studies correlating AMH serological levels with Sertoli cell function in bulls are needed.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"366-372"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10151779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. 黄体生成素分泌、排卵能力和卵母细胞质量的研究。

IF 1.7 4区生物学

Zygote Pub Date : 2023-08-01 DOI: 10.1017/S0967199423000230

Clara Slade Oliveira, Paola Maria da Silva Rosa, Bruna Rios Coelho Alves, Clara Ana Santos Monteiro, Gabriela Ramos Leal, Pedro Henrique Evangelista Guedes, Agostinho Jorge Dos Reis Camargo, Naiara Zoccal Saraiva

{"title":"Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers.","authors":"Clara Slade Oliveira, Paola Maria da Silva Rosa, Bruna Rios Coelho Alves, Clara Ana Santos Monteiro, Gabriela Ramos Leal, Pedro Henrique Evangelista Guedes, Agostinho Jorge Dos Reis Camargo, Naiara Zoccal Saraiva","doi":"10.1017/S0967199423000230","DOIUrl":"https://doi.org/10.1017/S0967199423000230","url":null,"abstract":"Induction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 ± 0.34 vs OV = 2.83 ± 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV groups. In conclusion, our results demonstrate a lower LH secretion profile in peripubertal Gir heifers prone to ovulate after induction protocol, and that oocyte quality is not affected on a short-term basis by ovulation itself.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 4","pages":"342-349"},"PeriodicalIF":1.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10151216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the addition of antifreeze protein type I on the quality of post-thawed domestic cat epididymal sperm. 添加I型抗冻蛋白对解冻后家猫附睾精子质量的影响。

IF 1.7 4区生物学

Zygote Pub Date : 2023-06-01 DOI: 10.1017/S0967199422000521

L P Alcaráz, P V S Pereira, T A Oliveira, L F L Correia, E M Vasconcelos, F Z Brandão, J M G Souza-Fabjan

{"title":"Effect of the addition of antifreeze protein type I on the quality of post-thawed domestic cat epididymal sperm.","authors":"L P Alcaráz, P V S Pereira, T A Oliveira, L F L Correia, E M Vasconcelos, F Z Brandão, J M G Souza-Fabjan","doi":"10.1017/S0967199422000521","DOIUrl":"https://doi.org/10.1017/S0967199422000521","url":null,"abstract":"Cryopreservation of domestic cat semen is mainly performed as a model for the establishment of endangered wild feline protocols. The supplementation of antifreeze protein type I (AFP I) to cryopreservation medium has shown improvement in frozen-thawed sperm quality in other species, but its effect on cat semen has not yet been tested. This study aimed to assess the addition of AFP I to cryopreservation medium in domestic cats. Sperm was obtained from the cauda epididymis of orchiectomized cats; sperm was then pooled in Tris buffer and allocated into three treatments, according to AFP I final concentration: 0 (control), 0.1, and 0.5 µg/ml. Nine replicates were cryopreserved in a two-step protocol and subsequently thawed at 37°C for 30 s. There was no difference (P > 0.05) among the control, 0.1 and 0.5 µg/ml groups for parameters such as motility, vitality, functional membrane integrity, mature chromatin, normal morphology, and sperm binding to egg perivitelline membrane. In the 0.5 μg/ml group only, percentages of live sperm with intact acrosome and of sperm with most inactive mitochondria (DAB III) showed a significant reduction, along with a tendency (P = 0.053) to an increase in the percentage of sperm with most active mitochondria (DAB II). In conclusion, the supplementation of 0.1 and 0.5 µg/ml of AFP I did not promote consistent beneficial effects on the overall sperm cryotolerance in domestic cats.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"31 3","pages":"240-245"},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9487044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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