Zygote最新文献

{"title":"The effect of rapamycin treatment on mouse ovarian follicle development in dehydroepiandrosterone-induced polycystic ovary syndrome mouse model.","authors":"Ecem Yildirim, Tugce Onel, Sami Agus, Elif Gunalan, Bayram Yilmaz, Mehmet Serif Aydin, Aylin Yaba","doi":"10.1017/S0967199424000388","DOIUrl":"10.1017/S0967199424000388","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is a complex reproductive and endocrine disorder affecting 5-10% of women of reproductive age, but the pathophysiology of PCOS still remains unknown. Here, the aim of our study was to analyze the effects of rapamycin treatment that may regulate impaired hormonal levels and folliculogenesis in dehydroepiandrosterone (DHEA)-treated PCOS mouse. We hypothesized that rapamycin may ameliorate the negative effects of PCOS in DHEA-induced PCOS mouse model. The target of rapamycin (TOR) gene product is a serine/threonine kinase that has been implicated in the control of cell growth, proliferation and autophagy, and rapamycin is a potent inhibitor of mTORC1 pathway. In this study, for the first time, mTORC1 and activation products are presented at protein and mRNA levels after rapamycin treatment in DHEA-induced PCOS mouse ovary. We showed that rapamycin treatment may regulate follicular development, hormonal levels and provide ovulation in DHEA-induced PCOS mouse. Additionally, we assessed decreased primordial follicle reserve, increased number of primary and secondary follicles, corpus luteum structure forms again after 10 days of rapamycin treatment. This study presented here suggests rapamycin treatment regulates hormonal phenotype and folliculogenesis in the ovary and also mTOR signalling pathway in granulosa cells of DHEA-induced PCOS mouse ovary which may have potential to attenuate understanding the mechanism of dominant follicle selection and anovulatory infertility.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"386-395"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different temperatures on the embryonic development of the Lebranche mullet Mugil liza","authors":"João Vitor de Azevedo Manhães, Douglas da Cruz Mattos, Rômulo Alves Strassburguer, Ulysses da Silva Palma, Fabio Carneiro Sterzelecki, Marco Shizuo Owatari, Caio Magnotti, Vinicius Ronzani Cerqueira","doi":"10.1017/s0967199424000285","DOIUrl":"https://doi.org/10.1017/s0967199424000285","url":null,"abstract":"<p>We herein investigated the influence of temperature on the embryonic development (from fertilisation to hatching) of <span>Mugil liza</span> larvae. For this purpose, oocytes (&gt;600 μm) and sperm were obtained from breeding stock at the laboratory of marine fish culture (LAPMAR). After fertilisation, 1200 eggs were distributed in 12 cylindrical experimental units of 400 mL under four different temperatures 18, 22, 26 and 30 ºC, all in triplicate. Every 15 min until hatching, about 10 eggs were randomly sampled in each treatment. The eggs were visualized and photographed, and the classification of embryonic stages was performed. Temperature influenced the main events of the embryonic development of <span>M. liza</span>. More accelerated development was observed according to the increase in temperature until the gastrula phase. At temperatures of 22 and 26 °C, embryonic development occurred from fertilisation to hatching of the larvae. In the 18 °C treatment, it was verified that most of the embryos ceased development during the final phase of cleavage and the beginning of blastula formation, while in the 30 °C treatment patterns of embryo malformation were also verified, with erratic divisions of the blastomeres, resulting in irregular cells. Unlike what was observed at a temperature of 18 °C, none of the embryos incubated at 30 °C reached the blastopore closure phase, stopping in the gastrula. The larvae hatched in the treatments at 22 and 26 °C were viable and exhibited intense swimming, with a large amount of reserve material (yolk) and an evident drop of oil.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"27 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol attenuates doxorubicin-induced toxicity during in vitro culture of mouse-isolated preantral follicles","authors":"G.A.L. Silva, A.P.O Monte, A.T.V. França, I.M. Mota, J.L. Oliveira Junior, K.O. Andrade, L.M. Souza, R.L.S. Silva, V.S. Guimarães, R.S. Barberino, R.C. Palheta Junior, J.E.J. Smitz, M.H.T. Matos","doi":"10.1017/s0967199424000315","DOIUrl":"https://doi.org/10.1017/s0967199424000315","url":null,"abstract":"<p>The aims of this study were to evaluate the doxorubicin concentration that induces toxic effects on <span>in vitro</span> culture of isolated mouse secondary follicles and to investigate whether resveratrol can inhibit or reduce this toxicity. Secondary follicles were isolated and cultured for 12 days in control medium (α-MEM<span>+</span>) or in α-MEM<span>+</span> supplemented with doxorubicin (0.1 µg/ml) or different concentrations of resveratrol (0.5, 2, or 5 µM) associated with doxorubicin (0.1 µg/ml) (experiment 1). For experiment 2, follicles were cultured in α-MEM<span>+</span> alone or supplemented with doxorubicin (0.3 µg/ml) or different concentrations of resveratrol (5 or 10 µM) associated or not with doxorubicin (0.3 µg/ml) (experiment 2). The endpoints analyzed were morphology (survival), antrum formation, follicular diameter, mitochondrial activity, glutathione (GSH) levels and DNA fragmentation. In the first experiment, doxorubicin (0.1 µg/ml) maintained survival and antrum formation similar to the control, while 5 µM resveratrol showed increased parameters, maintained mitochondrial activity and increased GSH levels compared to the control. In the second experiment, doxorubicin (0.3 µg/ml) reduced survival, antrum formation and follicular diameter compared to the control. Resveratrol at a concentration of 10 µM attenuated the damage caused by doxorubicin by improving follicular survival and did not present DNA fragmentation. In conclusion, supplementation of the <span>in vitro</span> culture medium with 0.3 µg/ml doxorubicin reduced the survival and impaired the development of mouse-isolated preantral follicles. Resveratrol at 10 µM reduced doxorubicin-induced follicular atresia, without DNA fragmentation in the follicles.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"20 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of calcium-sensing receptor (CaSR) expression and function in normal and abnormal human sperm and spermatogenic cells","authors":"Zhengli Qian, Keyan Luo, Mingzhe Zhang, Guanping Yao","doi":"10.1017/s0967199424000091","DOIUrl":"https://doi.org/10.1017/s0967199424000091","url":null,"abstract":"Summary The calcium-sensing receptor (CaSR) is a critical mediator of calcium homeostasis in various tissues. Its role in human reproduction, especially in sperm function and male fertility, remains not fully elucidated. This study investigates the expression patterns of CaSR in normal and abnormal sperm and spermatogenic cells and evaluates its potential effect on sperm motility and morphology. Using immunohistochemistry (IHC), quantitative PCR (qPCR), we assessed the expression levels of CaSR in normal sperm, spermatogonia, and cases of asthenozoospermia, oligozoospermia, and teratozoospermia. <jats:italic>In vitro</jats:italic> functional assays were performed to analyze the effects of CaSR modulation on sperm motility under varying conditions, including the presence of specific CaSR agonists and antagonists. Our study revealed distinct patterns of CaSR expression in normal sperm and spermatogonia compared with those in abnormal sperm samples, particularly in cases of asthenozoospermia, oligozoospermia, and teratozoospermia. A marked decrease in CaSR expression was evident in these abnormal samples, highlighting its significance in normal sperm functionality. Functional assays further elucidated the role of CaSR in sperm motility. Activation of CaSR through specific agonists enhanced sperm motility, while inhibition by antagonists led to reduced motility. Our findings suggest that CaSR plays a significant role in maintaining sperm functionality and that changes in its expression may be associated with male infertility. These insights into the molecular underpinnings of sperm physiology highlight CaSR as a potential therapeutic target for treating certain forms of male infertility.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"49 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The TLI system can help day-3 single cleavage embryo transfer to obtain comparative clinical outcomes to day-4 or day-5","authors":"Yue Ji, Linjun Chen, Fan Pan, Ruyu Jiang, Shanshan Wang","doi":"10.1017/s0967199424000212","DOIUrl":"https://doi.org/10.1017/s0967199424000212","url":null,"abstract":"Summary The aim was to explore whether the time-lapse imaging system can help day-3 single cleavage embryo transfer to obtain comparative clinical outcomes to day-4 or 5. The data of 1237 patients who underwent single embryo transfer from January 1, 2018, to September 30, 2020, in our reproductive medicine centre were retrospectively analysed. They were divided into the day-3 single cleavage-stage embryo transfer (SCT) group (<jats:italic>n</jats:italic> = 357), day-4 single morula transfer (SMT) group (<jats:italic>n</jats:italic> = 129) and day-5 single blastocyst transfer (SBT) group (<jats:italic>n</jats:italic> = 751) according to the different embryo transfer stage. The clinical and perinatal outcomes of the three groups were analysed and compared. The clinical pregnancy rates of the patients in the day-3 SCT group, day-4 SMT group and day-5 SBT group were 68.07, 70.54 and 72.04%, respectively. The live birth rates were 56.86, 61.24 and 60.99%, respectively. The monozygotic twin (MZT) rate in the day-3 SCT group was significantly lower than that in the day-5 SBT group (<jats:italic>P</jats:italic> = 0.049). Regarding perinatal outcomes, only the secondary sex ratio had a significant difference (<jats:italic>P</jats:italic> &lt; 0.05). After age stratification, no improvement was found in the pregnancy outcomes of patients &gt;35 years of age receiving blastocyst transfer. Our findings suggest that for patients with multiple high-quality embryos on day-3, prolonging the culture time can improve the pregnancy outcome to some extent, but it will bring risks. For centres that have established morphodynamic models, day-3 SCT can also achieve an ideal pregnancy outcome and reduce the rate of monozygotic twins and sex ratio.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"99 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microRNAs in the regulation of critical genes and signalling pathways that determine endometrial receptivity","authors":"Yumei Wang, Zhongxiang Ji, Ni Yao, Ximin Hu, Ran Zhou, Xingping Wang, Zhuoma Luoreng","doi":"10.1017/s0967199424000297","DOIUrl":"https://doi.org/10.1017/s0967199424000297","url":null,"abstract":"Summary Endometrial receptivity is the ability of the endometrium to accept embryos. Thus, endometrial receptivity dysfunction is an important factor leading to embryo implantation failure. A good endometrial receptivity provides a suitable environment for embryo implantation, improving the embryo implantation rate. The “implantation window” stage, or the receptive stage of the endometrium, is regulated by various hormones, genes, proteins and cytokines, among which microRNAs (miRNAs) and their target genes have a regulatory effect on endometrial receptivity. This review outlines the relationship between endometrial receptivity and pregnancy, the mRNAs and related signalling pathways that regulate endometrial receptivity, and the regulatory role of miRNA in endometrial receptivity, providing a deeper understanding of the regulatory mechanisms of miRNA on endometrial receptivity in humans and animals and reference for the endometrial receptivity-related research.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"21 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a PCR-based method to identify fetal sex during IVF cycles","authors":"Atieh Sadat Mousavi, Sadegh Amiri, Mehdi Mehdizadeh, Mehrdad Bakhtiari, Jamileh Sadat Mirsanei, Fatemeh Nikmard, Mahmood Barati, Fatemehsadat Amjadi","doi":"10.1017/s096719942400011x","DOIUrl":"https://doi.org/10.1017/s096719942400011x","url":null,"abstract":"One of the most recognizable cases of preimplantation genetic diagnosis (PGD) is X-linked diseases. Diagnosis of fetal sex is essential for couples who are known to be at risk of some X-linked disorders. The objective of this study was to discriminate between female (XX) and male (XY) embryos by detecting sex chromosomes-specific sequences in spent culture medium and comparing these results to PGD/CGH array results. It may open new window for the development of a non-invasive PGD method. 120 Embryo’s spent media from Day 3 and Day 5 embryos were collected. Modified phenol-chloroform solution was used for DNA extraction from spent media. Sex determination was performed using SRY, TSPY and AMELOGENIN evaluation through quantitative polymerase chain reaction (q-PCR) method. IBM SPSS and MedCalc were used for statistical analyses to compare sex determination of embryos by spent medium with PGD/CGH array results. Culture time was demonstrated to increase the DNA amount among day 5 embryos culture medium samples. Non-invasive PGD by means of spent culture medium gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% for sex determination. Results of sex determination using spent medium by q-PCR were consistent with the results of PGD/CGH array. Improvements in cell-free DNA extraction and PCR amplification procedures provide us an effective method to perform a PGD test without biopsy in the future, especially about X-linked diseases.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"7 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the impact of epigenetic mechanisms on offspring growth, production, reproduction and disease susceptibility","authors":"Pushpa Sindhu, Ankit Magotra, Vikas Sindhu, Pradeep Chaudhary","doi":"10.1017/s0967199424000224","DOIUrl":"https://doi.org/10.1017/s0967199424000224","url":null,"abstract":"Summary Epigenetic mechanisms, such as DNA methylation, histone modifications and non-coding RNA molecules, play a critical role in gene expression and regulation in livestock species, influencing development, reproduction and disease resistance. DNA methylation patterns silence gene expression by blocking transcription factor binding, while histone modifications alter chromatin structure and affect DNA accessibility. Livestock-specific histone modifications contribute to gene expression and genome stability. Non-coding RNAs, including miRNAs, piRNAs, siRNAs, snoRNAs, lncRNAs and circRNAs, regulate gene expression post-transcriptionally. Transgenerational epigenetic inheritance occurs in livestock, with environmental factors impacting epigenetic modifications and phenotypic traits across generations. Epigenetic regulation revealed significant effect on gene expression profiling that can be exploited for various targeted traits like muscle hypertrophy, puberty onset, growth, metabolism, disease resistance and milk production in livestock and poultry breeds. Epigenetic regulation of imprinted genes affects cattle growth and metabolism while epigenetic modifications play a role in disease resistance and mastitis in dairy cattle, as well as milk protein gene regulation during lactation. Nutri-epigenomics research also reveals the influence of maternal nutrition on offspring’s epigenetic regulation of metabolic homeostasis in cattle, sheep, goat and poultry. Integrating cyto-genomics approaches enhances understanding of epigenetic mechanisms in livestock breeding, providing insights into chromosomal structure, rearrangements and their impact on gene regulation and phenotypic traits. This review presents potential research areas to enhance production potential and deepen our understanding of epigenetic changes in livestock, offering opportunities for genetic improvement, reproductive management, disease control and milk production in diverse livestock species.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"459 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CellRox green fluorescence upon thawing on in vitro Bos taurus and Bos indicus embryos cryopreserved by slow freezing or vitrification","authors":"David Osornio, David Alejandro Contreras, Edgar Jimenez-Diaz, Tatiana Fiordelisio, Patricia López-Damian, José Francisco Martínez, Carlos Salvador Galina","doi":"10.1017/s0967199424000121","DOIUrl":"https://doi.org/10.1017/s0967199424000121","url":null,"abstract":"Summary The aim of this study was to compare the levels of reactive oxygen species (ROS) in <jats:italic>Bos taurus</jats:italic> and <jats:italic>Bos indicus in vitro</jats:italic> embryos cryopreserved using either slow freezing or vitrification. Embryos were divided into four groups based on subspecies and freezing method: <jats:italic>Bos indicus</jats:italic> slow freezing (BiSF; <jats:italic>n</jats:italic> = 8), <jats:italic>Bos indicus</jats:italic> vitrification (BiVT; <jats:italic>n</jats:italic> = 10), <jats:italic>Bos taurus</jats:italic> slow freezing (BtSF; <jats:italic>n</jats:italic> = 9), and <jats:italic>Bos taurus</jats:italic> vitrification (BtVT; <jats:italic>n</jats:italic> = 6). After thawing, the embryos were incubated with CellRox Green and images were obtained using a confocal microscope. The fluorescence intensity of each cell was measured and expressed as arbitrary units of fluorescence (auf) and compared using a multiple regression and unpaired <jats:italic>t</jats:italic>-test with α = 0.05. Results showed that subspecies and the freezing method significantly affected auf (<jats:italic>P</jats:italic> &lt; 0.001; <jats:italic>R</jats:italic><jats:sup>2</jats:sup> = 0.1213). <jats:italic>Bos indicus</jats:italic> embryos had higher auf than <jats:italic>Bos taurus</jats:italic> embryos, whether frozen by slow freezing (67.05 ± 23.18 vs 51.30 ± 16.84, <jats:italic>P</jats:italic> &lt; 0.001) or vitrification (64.44 ± 23.32 vs 47.86 ± 17.53, <jats:italic>P</jats:italic> &lt; 0.001). Slow freezing induced higher auf than vitrification in both <jats:italic>Bos taurus</jats:italic> (51.30 ± 16.84 vs 47.86 ± 17.53, <jats:italic>P</jats:italic> &lt; 0.001) and <jats:italic>Bos indicus</jats:italic> (67.05 ± 23.18 vs 64.44 ± 23.32, <jats:italic>P</jats:italic> &lt; 0.014). In conclusion, <jats:italic>Bos taurus</jats:italic> embryos had lower ROS levels when frozen using vitrification, while <jats:italic>Bos indicus</jats:italic> embryos had consistent ROS patterns regardless of the freezing method. However, <jats:italic>Bos indicus</jats:italic> embryos frozen by slow freezing tended to have a higher number of cells with elevated ROS levels.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"14 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature on the embryonic and larvae development of discus fish Symphysodon aequifasciatus and time of first feeding","authors":"Douglas da Cruz Mattos, Leonardo Demier Cardoso, Adriano Teixeira de Oliveira, Rafaela Screnci-Ribeiro, Bruno Olivetti de Mattos, Paulo Henrique Rocha Aride, Marcella Costa Radael, Jonas Henrique de Souza Motta, Manuel Vazquez Vidal","doi":"10.1017/s0967199424000236","DOIUrl":"https://doi.org/10.1017/s0967199424000236","url":null,"abstract":"Summary This study aimed to analyze the influence of different temperatures on the embryonic and larval development of discus fish <jats:italic>Symphysodon aequifasciatus</jats:italic> and determine the time required for the beginning of exogenous feeding. Eggs and larvae were obtained from natural spawns and distributed in five treatments: 24.0, 26.0, 28.0, 30.0, and 32.0 °C. To assess the developmental stages and embryonic structures, samples were taken at regular intervals and checked under an optical microscope. At the end of the experimental period, statistical analysis was performed, followed by Tukey’s test. As a result, it was possible to observe the significant effects of temperature on the variables. It was noted that the temperature accelerated the embryonic and larval development of the discus and also contributed to a reduction in the time between the incubation period and the feeding transition. It was also noted that the incubation of eggs and larvae at a temperature of 24.0 °C can cause damage to embryos, such as malformation of the body as well as anomalies in the circulatory system.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":"33 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}