IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1017/S0967199424000303

Shayesteh Mehdinejadiani, Zahra Khosravizadeh, Akram Alizadeh, Nahid Azad

引用次数: 0

Oocyte competence develops: nuclear maturation synchronously with cytoplasm maturation. 卵母细胞能力的发展：核成熟与细胞质成熟同步进行。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-18 DOI: 10.1017/S0967199424000169

Hossein Torkashvand, Ronak Shabani, Tayebe Artimani, Iraj Amiri, Shamim Pilehvari, Leila Torkashvand, Rana Mehdizadeh, Mehdi Mehdizadeh

{"title":"Oocyte competence develops: nuclear maturation synchronously with cytoplasm maturation.","authors":"Hossein Torkashvand, Ronak Shabani, Tayebe Artimani, Iraj Amiri, Shamim Pilehvari, Leila Torkashvand, Rana Mehdizadeh, Mehdi Mehdizadeh","doi":"10.1017/S0967199424000169","DOIUrl":"10.1017/S0967199424000169","url":null,"abstract":"Human oocyte maturation is a lengthy process that takes place over the course of which oocytes gain the inherent ability to support the next developmental stages in a progressive manner. This process includes intricate and distinct events related to nuclear and cytoplasmic maturation. Nuclear maturation includes mostly chromosome segregation, whereas rearrangement of organelles, storage of mRNAs and transcription factors occur during cytoplasmic maturation.Human oocyte maturation, both in vivo and in vitro, occurs through a process that is not yet fully understood. However, it is believed that the second messenger, cyclic adenosine monophosphate (cAMP), plays a pivotal role in the upkeep of the meiotic blocking of the human oocyte. Relatively high levels of cAMP in the human oocyte are required to maintain meiosis blocked, whereas lower levels of cAMP in the oocyte enable meiosis to resume. Oocyte cAMP concentration is controlled by a balance between adenylate cyclase and phosphodiesterases, the enzymes responsible for cAMP generation and breakdown.In addition to nuclear maturation, the female gamete requires a number of complicated structural and biochemical modifications in the cytoplasmic compartment to be able to fertilize normally. According to ultrastructural studies, during the transition from the germinal vesicle stage to metaphase II (MII), several organelles reorganize their positions. The cytoskeletal microfilaments and microtubules found in the cytoplasm facilitate these movements and regulate chromosomal segregation.The aim of this review is to focus on the nuclear and cytoplasmic maturation by investigating the changes that take place in the process of oocytes being competent for development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"421-428"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control of sperm penetration using stereumamide A derived from Trichaptum fuscoviolaceum in the in vitro fertilization of pig oocytes. 在猪卵母细胞体外受精过程中使用从毛癣菌（Trichaptum fuscoviolaceum）中提取的立体酰胺 A 控制精子穿透。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-25 DOI: 10.1017/S0967199424000327

Young-Joo Yi, Yoon-Ju Lee, Adikari Arachchige Dilki Indrachapa Adikari, Bong-Sik Yun

{"title":"Control of sperm penetration using stereumamide A derived from Trichaptum fuscoviolaceum in the in vitro fertilization of pig oocytes.","authors":"Young-Joo Yi, Yoon-Ju Lee, Adikari Arachchige Dilki Indrachapa Adikari, Bong-Sik Yun","doi":"10.1017/S0967199424000327","DOIUrl":"10.1017/S0967199424000327","url":null,"abstract":"Fungal metabolites are known to have potent and diverse properties such as antiviral, antidiabetic, antitumour, antioxidant, free radical scavenging, and antibacterial effects which can be utilized to treat diseases. In this study, we investigated the functional activity of stereumamide A (StA) derived from a culture broth of Trichaptum fuscoviolaceum during the in vitro fertilization (IVF) of pig oocytes, to determine its effects on sperm penetration. Oocytes matured in vitro were fertilized in the absence or presence of varying concentrations of StA (0-50 μg/ml StA). When StA was directly added into the IVF medium, significantly lower fertilization rates were seen with the 20 or 50 μg/ml StA (2.0-17.5%) treatments compared with those of 10 μg/ml StA or the controls (60.9-62.3%), whereas StA had no influence on the survival of oocytes and spermatozoa throughout the IVF process. For evaluating the control of sperm entry, mature oocytes were pre-incubated in a medium containing 20 μg/ml StA for 1 h, and then IVF was subsequently performed. The incidence of polyspermy was significantly reduced when oocytes were pre-incubated with StA (15.0% vs. 50.4-57.5% in controls). In conclusion, sperm penetration was inhibited in the medium in the presence of StA during IVF, while StA did not affect sperm motility and fertility competence. Fertilization was controlled when mature oocytes were incubated with StA prior to IVF, suggesting the possible use of the fungal metabolite in assisted reproductive technology for humans and animals.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"354-359"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress. 用 GYY4137 处理人类精子可提高精子活力和抗氧化能力。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-30 DOI: 10.1017/S0967199424000340

Yan Huang, Runxin Gan, Min Zhang, Dewei Lin, Yi Cheng, Xinyu Guo

{"title":"Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress.","authors":"Yan Huang, Runxin Gan, Min Zhang, Dewei Lin, Yi Cheng, Xinyu Guo","doi":"10.1017/S0967199424000340","DOIUrl":"10.1017/S0967199424000340","url":null,"abstract":"Hydrogen sulfide (H2S) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing H2S compound, on sperm damage induced by H2O2. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by H2O2. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 μM H2O2, followed by incubation with varying concentrations of GYY4137 (0.625-2.5 μM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that H2O2 adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against H2O2-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"360-365"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the effect of serotonin-activated semen washing medium on sperm motility at the molecular level: a pilot study. 血清素激活精液洗涤介质对精子活力分子水平影响的调查：一项试验性研究。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-11 DOI: 10.1017/S0967199424000406

Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek

{"title":"Investigation of the effect of serotonin-activated semen washing medium on sperm motility at the molecular level: a pilot study.","authors":"Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek","doi":"10.1017/S0967199424000406","DOIUrl":"10.1017/S0967199424000406","url":null,"abstract":"In Assisted Reproductive Technologies (ART), efficient sperm preparation is vital for successful fertilization, with washing media enhancing the process. This pilot study examines the molecular-level impact of a new serotonin-containing sperm-washing medium (Prototype) on sperm motility and ROS metabolism, comparing it with commercially available media (Origio and Irvine). Semen samples from thirty-one individuals underwent preparation using the swim-up method post-semen analysis. Each sample was separately washed with the Prototype, Origio and Irvine mediums. ROS formation was determined through flow cytometric, and AT2R and PRDX2 protein levels, associated with sperm motility, were assessed via Western blot. Statistical evaluation compared the findings among the three outlined media. Significant differences were found among three washing media in terms of total and progressive motility. The Prototype medium showed the highest increase in both total (66%) and progressive motility (59%), while the control group exhibited the lowest increases (41% and 27.7%, respectively). Regarding ROS levels, the prototype (11.5%) and Origio (10.7%) groups demonstrated a notable decrease, contrasting with Irvine (25.8%). Molecular assessment revealed a significant elevation in AT2R protein levels in the prototype medium (59%), compared to other media. Additionally, an increase in PRDX2 protein levels was observed in the prototype medium, although this didn't reach statistical significance. Serotonin-activated washing media for sperm preparation can be a suitable choice for selecting high-quality sperm in ART. A broader molecular analysis with a larger sample size is required to explore the mechanisms and effectiveness of using a serotonin-containing sperm-washing medium in routine ART.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"396-404"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of L-carnitine in the control of oxidative stress and lipid β-oxidation during in vitro follicle growth, oocyte maturation, embryonic development and cryopreservation: a review. 左旋肉碱在体外卵泡生长、卵母细胞成熟、胚胎发育和冷冻保存过程中控制氧化应激和脂质β氧化的作用：综述。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-07 DOI: 10.1017/S096719942400039X

Allana Maria Freire Leitão, Bianca Regia Silva, Efigênia C Barbalho, Lais R M Paulino, Francisco das Chagas Costa, Fabricio Sousa Martins, Jose Roberto V Silva

引用次数: 0

Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus). 通过经阴道取卵术从 FSH 刺激的塔帕卡尔供体母牛（Bos indicus）中获取卵母细胞，对其发育能力进行形态分子评估。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-29 DOI: 10.1017/S0967199424000376

Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan

{"title":"Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus).","authors":"Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan","doi":"10.1017/S0967199424000376","DOIUrl":"10.1017/S0967199424000376","url":null,"abstract":"The study was conducted on indigenous Tharparkar cow (Bos indicus) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB+VE as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in Bos indicus cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB+VE oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"376-385"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the proliferative activity of germline progenitor cells in the adult ovary of the bat Artibeus jamaicensis. 鉴定蝙蝠Artibeus jamaicensis成体卵巢中生殖祖细胞的增殖活性。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI: 10.1017/S0967199424000364

Tania J Porras-Gómez, Norma Moreno-Mendoza

{"title":"Identification of the proliferative activity of germline progenitor cells in the adult ovary of the bat Artibeus jamaicensis.","authors":"Tania J Porras-Gómez, Norma Moreno-Mendoza","doi":"10.1017/S0967199424000364","DOIUrl":"10.1017/S0967199424000364","url":null,"abstract":"Until a few years ago, it was assumed that oocyte renewal did not take place in the ovary of adult organisms; however, the existence of germline progenitor cells (GPCs), which renew the ovarian follicular reserve, has now been documented in mammals. Specifically, in the adult ovary of bats, the presence of cells located in the cortical region with characteristics similar to GPCs, called adult cortical germ cells (ACGC), has been observed. One of the requirements that a GPC must fulfil is to be able to proliferate mitotically, so the evaluation of cell proliferation in ACGC is of utmost importance in order to be able to relate them to a parental lineage. Currently, there are several methods to determine cell proliferation, including BrdU labelling or the use of endogenous proliferation markers. Thus, the aim of this work was to evaluate the proliferative activity of ACGC in the adult ovary of the bat Artibeus jamaicensis, using different proliferation markers and correlating these with the protein expression of the transcription factor Oct4 and the germ line marker Ddx4. We found that the expression pattern of the proliferation markers BrdU, PCNA, Ki-67 and pH3 occurs at different times of the cell cycle, so co-localization of two or more of these markers allows us to identify proliferating cells. This allowed us to identify ACGC with proliferative capacity in the adult ovary of A. jamaicensis, suggesting that GPCs renew the follicle reserve during the adult life of the organism.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"366-375"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes. 补充硼酸可通过相关的多能基因和抗氧化基因促进体外生产的小鼠胚胎的发育。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-21 DOI: 10.1017/S0967199424000261

Ali Cihan Taskin, Ahmet Kocabay, Seref Gul, Gizem Nur Sahin, Sercin Karahuseyinoglu, I Halil Kavakli, Ibrahim Sogut

{"title":"Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes.","authors":"Ali Cihan Taskin, Ahmet Kocabay, Seref Gul, Gizem Nur Sahin, Sercin Karahuseyinoglu, I Halil Kavakli, Ibrahim Sogut","doi":"10.1017/S0967199424000261","DOIUrl":"10.1017/S0967199424000261","url":null,"abstract":"Boric acid (BA) is an important mineral for plants, animals and humans that assists metabolic function and has both positive and negative effects on biological systems. The present study aimed to investigate the effects of different concentrations of BA added to the culture media, the quality and in vitro development potential of mouse embryos. Superovulated C57Bl6/6j female mice were sacrificed ∼18 hours after human chorionic gonadotropin (hCG) injection. Single-cell-stage embryos were collected from the oviduct, divided into experiment groups and cultured in embryo medium with supplemented BA+ in 5% CO2 at 37 °C until 96 hours at the blastocyst stage. The blastocyst development rates of 0, 1.62 × 10-1, 1.62 × 10-2, 1.62 × 10-3 and 1.62 × 10-4 µM BA were 51.52%, 73.47%, 77.36% and 81.13%, respectively. The in vitro development rates were significantly higher in the 1.62 × 10-3 (p < 0.05) and 1.62 × 10-4 µM BA groups than in the control group (p < 0.001). These results indicated that low BA doses influenced embryo development by positively affecting in vitro development rates, embryo cell numbers, biochemical parameters and development at the molecular level by pluripotent and antioxidant genes. Therefore, BA seems to play an important role on in vitro embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"348-353"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and transcriptomic evaluations of infertile or subfertile Arunachali yak sperm. 不育或亚不育阿鲁纳恰里牦牛精子的基因组和转录组评估。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-17 DOI: 10.1017/S0967199424000194

Pranab Jyoti Das, Aneet Kour, Jyotika Bhati, Dwijesh Chandra Mishra, Mihir Sarkar

{"title":"Genomic and transcriptomic evaluations of infertile or subfertile Arunachali yak sperm.","authors":"Pranab Jyoti Das, Aneet Kour, Jyotika Bhati, Dwijesh Chandra Mishra, Mihir Sarkar","doi":"10.1017/S0967199424000194","DOIUrl":"10.1017/S0967199424000194","url":null,"abstract":"Sperm infertility or subfertility is detrimental to the precious highland germplasm like yak whose population has been gradually declining in India. Understanding the 'omic' landscape of infertile or subfertile yak sperm can reveal some interesting insights. In an attempt to do the same, this study considered the semen of infertile or subfertile yak bulls for whole-genome and transcriptome evaluations. DNA sequencing revealed that the yak sperm genome contains the necessary genes to carry out all the important biological processes related to the growth, development, survival and multiplication of an organism. Interestingly, RNA Seq results highlighted that genes like VAMP7, MYLK, ARAP2 and MARCH6 showed increased expression, while biological processes related to immune response (GO:0043308, GO:0002447, GO:0002278, GO:0043307, GO:0043312, GO:0002283, GO:0043299 and GO:0002446) were significantly overrepresented. These findings hint at a possible role played by immune system in regulating infertility or subfertility in yaks. Further, in-depth studies can validate these findings and help in improving our biological understanding in this area.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"341-347"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zygote最新文献